eChapters

1665 Robert Hooke had successfully invented the microscope. Because of this discovery, Robert Hooke was the first one to have a close look of a cell appears to be. His description of these cells was published in Micrographia. However, the cell walls observed by Hooke gave no indication of the nucleus and other organelles found in most living cells.

1674 Anton van Leeuwenhoek witnessed a live cell (plant) under a microscope.

1775 Antoine Lavoisier first proposed a mechanism for photosynthesis, a process wherein plants plants take in carbon dioxide and release oxygen. Lavoisier was also the first to investigate cell respiration in animals.

1777-83 Chemistry became dominated by the phlogiston theory, or the hypothetical principle of fire wherein all the combustible material was part composed. In this principle, burning (oxidation) was caused by liberating phlogiston, with ash as the dephlogistigated substance.

1836 The proponent of the cell theory in Biology, Theodore Schwann, proposed that the process of fermentation is solely limited to living yeast cells in 1836. Liebig did not agree to this and instead, he proposed another alternative theory of fermentation.

1856 Louis Pasteur opposed Liebig’s chemical theory. In his experiment, he showed that fermentation depends highly on the physiological functions that occur in bacteria and in living yeast cells. This work of Pasteur in 1856 received general recognition.

1860s The view on the chemistry of life highly different from the chemistry of nonliving things. During this period, the view is that the gelatinous and homogenous form of matter in organisms or more commonly known as the protoplasm carries out all the intracellular processes. These include respiration, biosynthesis of molecules, and the breakdown of matter.

Embry Transfer Technology (ETT)

Definition: The process of removing one or more embryos from the reproductive tract of a donor female and transferring them to one or more recipient females.

A Hospital Management System not only provides an opportunity to the hospital to enhance their patient care but can also increase the profitability of the organization. These kinds of applications enable hospitals and doctors to serve their patients better. Computers are being extensively used for routine administrative purposes by health care professionals.

In veterinary medicine, hospital management software is designed for all types of practices, ranging from small, single-vet ones to large ones with multiple locations and vets, and covers a wide range of hospital administration and management processes. It is an integrated end–to-end management system that provides relevant information across the hospital to support effective decision making for patient care, administration & critical financial accounting in a seamless flow. Specific areas of application include scheduling of appointments, registration of cases including client and patient data, case history and electronic medical records, health care reports, reminders, inventory controls, reports and accounting.

Foot-and-Mouth Disease

Loeffler and Frosch in 1897. Apthovirus of Picornaviridae. Strain O, A, C, Asia-1, SAT-1, 2, 3.

Cattle as Indicator host, Pig as amplifier, Sheep as maintenance host.

Musculature of heart in calves will have striped appearance termed as TIGER HEART.

Panting is common feature or sequel following FMD due to disturb endocrine function.

Preferred cell line for virus isolation in BHK-21.

Our general environment is loaded with tiny invisible living things that influence us consistently. Microbiology is a specialized area of science that deals with living things typicallytoo little to be seen without magnification. Suchmicroscopic life forms are collectively referred to as microorganisms. Microbial kingdom incorporates the study of bacteria, rickettsiae, viruses, fungi, algae and protozo a. Virology the study of viruses, mycology study of fungi and protozoology deals with protozoa.Credit for prologue to microbial world goes to ‘Antony van Leeuwenhoek’ and to vistas of microbial activities to ‘Louis Pasteur’ and ‘Robert Koch’. This period is reffered as the “Golden Era” of microbiology on account of the significant discoveries viz: microbial fermentations, discovery of various disease-causing agents, microbes’ cultivations, differences in metabolic activities, development of attenuated strains and their use as immunoprophylactic agents. The techniques used to study bacteria and different microorganisms embody direct microscopic examination, cultivation, biochemical tests, animal inoculation, serological reactivity and recent molecular biological techniques.Definitive evidence for the cause of a given infection is established by Koch that are still used to this day to definitively prove that a microorganism causes a particular disease. Koch’s four postulates are:

Veterinary Nuclear Medicine (VNM)

Veterinary Nuclear Medicine (VNM) is a specialized field that uses radioisotopes to detect, diagnose, and treat diseases. Gamma scintigraphy involves injecting radioactive tracer pharmaceuticals into the animal’s body and imaging them as they move through the body using a gamma radiation-sensitive camera. This technique provides a 2D or 3D image of the whole body or a specific organ, allowing for the estimation of the quantitative distribution within the region of interest (ROI). Advancements in gamma scintigraphy have allowed for the acquisition of information about both static and dynamic high-resolution images, such as heart wall motion, blood flow, and myocardial perfusion. Unlike other diagnostic modalities viz X-rays, ultrasonography, and CT; gamma scintigraphy offers functional information of specific organs. The most commonly used gamma radiation-emitting isotope is 99m-Technetium (99mTc), which emits a gamma energy of 98% 140-keV photon with only 10% internal conversion. Other radionuclides used in veterinary nuclear medicine facilities include iodine (123I and 131I), Gallium (67Ga), and Thallium (201Ti).

This chapter explores the essential techniques used in veterinary parasitology to collect, identify, and diagnose parasitic infections. These methods are crucial for understanding general animal health, the complex relationships between parasites and their hosts and for tracking disease outbreaks, that will ultimately allow the development of effective control strategies. Whether in a research lab or a clinical setting, the techniques discussed here are widely used to detect and study parasites that impact both domestic and wild animals.
2.1 Collection of Specimens for Parasitological Examinations
Different species of parasites affects almost all parts of the host. These organisms can inhabit virtually every organ system of the host animal, from the brain and lungs to the digestive tract and skin. Therefore, a thorough examination of both living animals and post-mortem cases is essential for collecting parasites and determining cause of death (when applicable). When collecting from live animals, samples should be collected after through exanimation of the animals and following the standard procedure. The collection of correct samples, use of appropriate transport media and proper handling of specimens are crucial factors that directly influence diagnostic accuracy.
The type of specimen collected depends on the methods of identification and diagnosis. For example, diagnosis of blood parasites requires specific types of samples: peripheral blood smears for microscopic examination of hemoprotozoans, serum or plasma for ELISA and other serological tests, and unclotted whole blood for molecular diagnostic methods such as PCR and qPCR.

Parasite- The word parasite is derived from the Greek. Para- beside and sitos- food. Parasites are those living organism which reside on or inside the body of some other species of living organism.

Host- Those organisms which provide food shelter and conducive environment to parasite for their growth, development and reproduction. e.g. Tape worm in small intestine of man and animals.

Disease = dis + aise (see + ease) old French word

Transmission- Movement of biological agents (Virus, Bacteria, Rickettsial, Fungi & Parasites) from one place to another place via vector or vehicle/ tools

Vector- Latin word- bearer

Vector is a living organism (usually an arthropod) which transfers infective forms of a parasite from one host to the other.

Pathology is derived from Greek word (Pathos=disease + logos=study). It is study of structure and function of body in disease.

Veterinary Pathology is the study of anatomical, chemical and physiological alterations in comparison to normal due to disease in animals. Pathology has responsibility to forms a vital bridge between preclinical sciences (Anatomy, Physiology, Biochemistry) and clinical branches of medicine and surgery.

General Pathology deals with the basic alterations of tissues as a result of disease. e.g. fatty changes, thrombosis, amyloidosis, embolism, necrosis etc.

Systemic Pathology is related to the alterations in tissues/organs of a particular system. e.g. respiratory system, genital system etc.

Special Pathology is the application of the basic reactions of gene general pathology to various specific diseases.

Gross or Macroscopic Pathology is the examination of tissue without the help of a microscope.

Chemotherapy

Chemotherapy is branch of pharmacology that deals with study of chemical substance intended to kills or inhibits the growth of invading microorganism or foreign cell without harm to host cells. Paul Ehrlich coined the term chemotherapy and is known as father of chemotherapy. Gerhard Johannes Paul Domagk discovered sulphonamide (sulfonamidochrysoidine). First commercially available sulphonamide marketed in the form of Prontosil dye, for which he received the 1939 Nobel Prize in Physiology or Medicine. SelmanAbraham Waksman discover streptomycin and several other antibiotics from soil actinomycetes and actinomycetes is called treasurer house of antibiotics.

α-lactam antibiotics- includes penicillins, cephalosporins, carbapenems, monobectum and beta-lactamase inhibitors. It inhibits bacterial cell wall synthesis due to inhibition of transpeptidation reaction thus inhibits peptidoglycan synthesis. Penicillins contain 6-animopenicillanic acid (((lactam ring + thiazolidine ring) eg. natural penicillins, beta-lactamase-resistant agents, aminopenicillins, carboxypenicillins, and ureidopenicillins. Discovery of penicillin was done by Alexender fleming from penicillium mold in 1928 from Penicillium notatum. Now a day’s Penicillium chrysogenum mold is a major source of penicillin production. Benzathine penicillin G is a repository salt of penicillin. Probenicid competitively inhibit renal tubular secretion of penicillin and increase the half- life of penicillin. Procaine penicillin G should not administer by i.v. route due to adversely affect the cardiac conducting system. Beta-lactamses inhibitors are calvulenic acid (Streptomyces clavuligerus), salbactum, tazobactam. They are called suicidal /progressive inhibitors. Main adverse effect associate with penicillin is immediate type of hypersensitivity reaction due to Antigen+ IgE antibody reaction.

Veterinary physiology deals with the study of normal functions of animal systems. It includes the study of how animals work and the physical and chemical processes that occur within animals. The examples of various biological processes in animals include gas exchange, blood and circulation, osmoregulation, digestion, nervous and muscle systems, respiration, growth, reproduction, endocrine function and how animals behave and function in different environmental conditions. This also includes the possible manipulation of the reproductive processes by means of hormone manipulation techniques for more efficient livestock and poultry production.

Zoonoses

Zoonoses are those diseases and infections which are transmitted between vertebrate animals and humans under natural conditions. The emergence of a pathogen into a new host species is called disease invasion or “disease emergence”.

Anthrax is a notifiable disease and primarily occurs in herbivores but it is a type of bio-terrorism agent and called as malignant pustule, malignant edema, woolsorters disease, ragpickers disease, maladi charbon, splenic fever etc. Causative agent is Bacillus anthracis contains vegetative spore and lethal dose is 2,500 to 55,000 spores.

Brucellosis is one of the world’s most widespread zoonoses caused by the genus Brucella. In human it is called Malta fever, Undulant fever, Mediterranean fever, Rock Fever of Gibraltar and Gastric Fever while in animals it is called Bang’s disease, Enzootic abortion, Epizootic abortion, Slinking of calves, Ram epididymitis and Contagious abortion etc. it is also called occupational disease.

Leptospirosis is a disease of animal sand humanbeings caused by genus Leptospira and called as Weil’s syndrome, Canicola fever, Canefield fever, 7-day fever, Rat Catcher’s Yellows, Autumn fever, black jaundice). Fletcher’s EMJH media is used to culture the organism.

Veterinary surgery: The surgery which is practiced on animals. It deals with the treatment of deformities, injuries, or disease conditions by manipulation with the hands. The term “Surgery” is derived from a Greek word – Chirurgia (Cheir – Hand, Ergon – work).

The papyri of Egypt contained information on treatment of fractures and superficial wounds.

In Vedic era, Salihotra and Palakapya were the greatest ancient Indian veterinarians. Salihotra treated horses and Palakaya elephants.

“Susruta Samhita’ is the earliest known work dealing with the medical science especially surgery.

Hobday wrote first comprehensive text on surgical diseases of the dog and cat.

The theory and practice of asepsis was introduced by Joseph Lister.

W.S. Halsted introduced the principles of surgery and use of rubber gloves.

Q1. Which of the following is semisynthetic product:

1. Chlortetracycline
2. Chloramphenicol
3. Tetracycline
4. Oxytetracycline

Q2. Natural source of amphotericin B is:

1. Streptomyces aureofaciencs
2. Streptomyces nodosus
3. Streptomyces erythreus
4. Streptomyces venezuelse

Q3. Following is /are mitotic inhibitor

1. Paclitaxel
2. Griseofulvin
3. Vinblastine
4. All of the above