eChapters

Plant breeding is the purposeful manipulation of characters in plants to create new genotype with a set of desired characters. Hence, it may basically be referred to as designed manipulation of the heritable variation for the character concerned. Living organisms possess the remarkable capacity to convert the non-living materials into living staff, which results in movement, growth, metabolism and reproduction, and thus continuity of living organisms from one generation to the next is accomplished. The continuity of life through transmission of characters is the subject of the study of heredity, while the resemblances and differences between individuals of the same or different generations is the concern of the study of variation. These two universal processes of continuity and of change, which are referred to as “Heredity” and “Variation” are primary consequences of all organisms. Organized study of these two biological processes is the science of Genetics.
Plant breeding, which may be termed as evolution of plant type directed by the will of mankind, is basically the process of selection of character(s) from heritable variation. Desirable heritable variations can also be induced through some genetic consequences like, hybridization, segregation, mutation, changing the number of chromosomes, etc. So frame of plant breeding lay on three basics:

15.1 Introduction

The laws governing the flow of water in wells are referred to as the hydraulics of wells. Just like surface water flows from a higher elevation to a lower elevation by gravity, groundwater also flows from a region of higher water table to a region of lower water table. This difference of water table measured by any two piezometers divided by the longitudinal distance between them is called as hydraulic gradient. Hydraulic gradient plays a dominant role in deciding the flow of groundwater. This hydraulic gradient together with the hydraulic conductivity of the aquifer material forms the main law of well hydraulics called as Darcy’s law. An understanding of Darcy’s law together with some other laws as discussed subsequently in this chapter is essential in order to know the discharge of a well in a particular formation. Darcy’s law is valid for studying groundwater flow in alluvial formations. However, it is not valid for aquifers having hard rock areas.

Earth provides enough to satisfy every man’s need but not every man’s greed.
—Mahatma Gandhi
The term ‘environment’ is derived from French word ‘environner’, which literary means ‘to surround’ or ‘to encircle’ i.e. everything either living (plants, animals, microbes) or nonliving (soil, water, air, temperature, humidity etc.) surrounding us makes our environment. The universe is full of uncountable number of stars, planet and satellites, which has specific type of environment but earth is the only known planet in the universe that contains life supporting environment. The earth environment is a collective term for the nature or ecosystems, that we use and benefit from on a local, national and global scale. The earth ecosystems provide number of benefits called ecosystem services, free of cost.

Salinity is a measure of the total concentration of dissolved solids, which is usually expressed in parts per thousand (ppt). Chloride, sulphate, bicarbonate, and bromide are anions (- charged). Cations that are positively charged are sodium, magnesium, calcium, potassium, and strontium. The main solids are sodium and chloride.

1.     Preparation of Liquid and Solid Media
    
2.     Isolation of Pure Culture
    
3.     Analysis Bacterial Growth Curve
    
4.     Study of Effect of Carbon Source on Bacterial Growth
    
5.     Gram Staining
    
6.     Aseptic Cell Transfers
    
7.     Prokaryote Cell Number by Dilution Plating
    
8.     Cell Mass by Measurement of Turbidity
    
9.     Isolation of Bactriophage from Sewage Sample
    
10.     Standard Plate Count
    
11.     Imvic Test
    
12.     Antibiotic Sensitivity Test on Microbes
    
13.     Study of Transformation In Bacteria
    
14.     Study of Bacterial Conjugation
    
15.     Effect of pH and Temperature on Bacterial Growth
    
16.     Immobilization of Yeast Cells and Alcohol Production
    
17.     Citric Acid Production using Aspergillus Niger
    
18.     Alcohol Production from Yeast and Estimation of Alcohol

Introduction

Today, according to Global Assessment of Land Degradation and Improvement (GLADA), nearly one forth area of our world land has been degraded (Arora 2019). The United Nations Convention to Combat Desertification (UNCCD) is leading the issues of soil or land degradation to restore valuable natural resources for achieving a land degradation neutral world by 2030 (Cowie et al., 2018; IPBES, 2018; Bhattacharya, 2020). Land degradation neutrality (LDN) can be achieved primarily through adoption of sustainable land management practices. Soil organic carbon (SOC) plays a multifunctional role and is sensitive to land management practices and a key indicator of soil quality, soil health and LDN. Soil is a major reservoir of terrestrial C in the biosphere and plays an important role in global C cycle. Therefore, a little change in SOC pool can significantly influence the atmospheric CO2 concentration. In general, 1 Pg of SOC stock is equivalent to about 0.47 ppm of CO2 in the atmosphere, and vice versa (Lal, 2018). Besides this it maintains overall soil sustainability and enhances food security.

Achieving carbon neutrality in Indian soils is a major challenge. Because, productive land goes out of cultivation and marginal or degraded land comes under cultivation due to urbanization, industrialization and various development activities. Also, it is very difficult to increase SOC because climatic condition prevails with a high rate of decomposition. According to an UN report, India will be the most populated country in the world and expected to add 273 million people between 2019 and 2050 which will exert huge pressure on natural resources, particularly soil and water to fulfil the increasing food demands of such ever-growing population. The extensive use of natural resources along with intensive cultivation will enhance greenhouse gases (GHGs) emission, slow down agricultural productivity, enhance land degradation, and decline factor productivity of major production systems. Due to change in distribution of rainfall and concurrent rise in temperature owing to climate change, the fertile top soil becomes more prone to degradation.

SYNOPSIS

Food security is a complex issue, linked to availability and access to food, malnutrition in the population, economic development, environment, and local and global trade. Malnutrition hinders healthy growth and proper development of the human immune system affecting neurological and cognitive development especially in children.

Fabrication: The act of building or putting together. Forming into a whole by uniting or assembling of parts.

Face lathe: A short-bed, deep gap lathe designed for the machining of large, flat surfaces.

Face milling: Milling flat surfaces perpendicular to the rotational axis of the cutting tool.

Faceplate or face chuck: A circular plate for attachment to the spindle in the headstock of a lathe. Work may be clamped or bolted to it. The slots engage the tail of the lathe dog.

Factor of safety: The number, which results by dividing the ultimate strength of a material by the actual stress on a sectional area.

Factor of stress intensity: The ratio of the maximum stress to which a structural member can be subjected to the maximum stress of safety.

Post-harvest deterioration primarily means the loss of commodity after harvest. It is one of the serious problems for producers, distributors and consumers. Post-harvest deterioration of perishables is the spoilage of fruits and vegetables after harvesting. Perishables are the feed commodities with moisture content between 50 and 90%. Fruits with the size of 5 g to 5 kg or more, high to very high rate of respiration, high generation of heat, soft texture, easily damageable nature and shorter shelf life are more prone to the microbial damage. The post-harvest losses are caused either by pathological, physiological and mechanical means or combination of all these. These perishables because of having minerals, vitamins, and photochemicals (essentially required for the human health) become victim to the several biotic and abiotic factors easily. Once these fruits are detached from the plant are deprived of essential ingredients as hormones, nutrients, water and other requisites. These biotic and abiotic factors apart from rendering the produce unfit for human consumption reduce the aesthetic value, substantial decrease in food value and also organoleptic quality of the vegetables. The fleshy produce are prone to the exogenous agencies such as fungi, bacteria, etc. as well as other endogenous factors such as respiration, senescence, etc. which may lead to poor quality of the produce and also affect market quality. These perishables continue to respire and may carry out various biochemical activities, leading to ripening subsequently to senescence and making them more susceptible to microbial damage. In addition, tropical conditions prevailing in India, which are characterized by high temperature conditions, are optimal for fungal and bacterial infections. In India, the post-harvest losses of fresh fruits and vegetables are estimated to be about 30%. The post-harvest losses have been recorded are 5-20% at farmer’s field due to pre-harvest infection and sorting, 15-20% due to faulty packaging, 30-40% during transportation and 30-40% during marketing.

Abstract

Potato in India was introduced as a long day temperate crop which found suitable climate in the higher hills of the country. Since then it is grown widely in the Indo-Gangetic Plains, the Plateau and the Himalayan regions in north and the Nilgiris hills in south of India. The Central Potato Research Institute (CPRI), Shimla has developed 49 potato varieties by the conventional breeding for suitable cultivation of potato in different potato zones of India. A number of technologies for ware- and quality seed- potato production, storage and processing have been developed/standardized for adoption by the end users. These technologies have contributed immensely towards multiple increase in area, production and productivity of potato during last sixty years. Apart from conventional tools, modern biotechnological tools like whole genome sequencing technology, transgenics technology, molecular markers, RNAi and microarray technology have are also being employed in potato improvement.

Introduction

Value chain analysis has gained considerable popularity in recent years. Although many definitions are applied, value chains essentially represent enterprises in which different producers and marketing companies work within their respective businesses to pursue one or more end markets. Value chain participants sometimes cooperate to improve the overall competitiveness of the final product, but may also be completely unaware of the linkages between their operation and other upstream or downstream participants. Value chains therefore, encompass all of the factors of production including land, labor, capital, technology, and inputs as well as all economic activities including input supply, production, transformation, handling, transport, marketing, and distribution necessary to create, sell, and deliver a product to a certain destination.

Root and Tuber crops are the most important food crops of man after cereals. Tuber crops are efficient producers of carbohydrates especially under suboptimal conditions of rainfall, fertility and low input conditions encountered in tropics. The group contributes about 6 % of the world’s dietary calories. In fact, one out of 6 kg of human food comes from roots and tubers. Tuber crops find an important place in the dietary habits of small and marginal farmers especially contributing to food security of tribal populations. Tuber crops grown in India include Cassava, Sweet potato, Dioscorea, Colocasia, Amorphophallus and minor tuber crops like Chinese potato, Arrowroot, Canna, Yam bean etc. The biotechnology can contribute to the solution of the major problems and realize greater benefits for tuber crops.

Forage harvesting is more complex as compared to grain crops. Forage crop is of great bulk and mass, containing 70-80% water when first harvested. For storage it must be dried, either naturally or artificially, to a safe moisture content of about 20 to 25%. Due to low product value it limits the economic feasibility of mechanization of harvesting operation. The mowers are used to cut the crop and windrow in the field, which is manually collected for further chopping. The forage harvester consists of a combination of plant-cutting unit and a chopping unit and is called field forage harvester. The field forage harvester performs the functions of both row binder and silage cutter, as it severs the standing stalks from the ground and chops them into silage in one continuous operation in the field. It can be grouped into two according to the mechanism used to cut the crop viz. field choppers and flail harvesters.

Cities or urban conglomerations are fast developing and where the rate of land availability has infinitesimally small; vertical expansion and taller buildings have emerged and are defining the city skyline. Though this may speak well of the economic development, but the increase in hard paved areas and roads may spell trouble for the environment as urban heat island (UHI) effect. The causes of Urban Heat Island are primarily due to the absorption of solar radiation (Short wave) by building/urban materials that is subsequently re-radiated (long wave) to the surroundings. In addition, heat generated from the use of air- conditioning coupled with the greenhouse effect of pollutants also contribute to the increase in temperature. Such increase in temperature with the presence of air pollutants can result in the accumulation of smog, damages the natural environment and jeopardizes human health. It also costs consumers more money because it takes more energy to cool buildings. Who can forget the regular news of Beijing repeatedly topping the list of most polluted cities and fresh air is available in cans!

Fresh air cans are in no means a firm solution. Plants are atmosphere cleansers, can serve to filter carbon dioxide and other toxins in environment, cool the air by transpiration and also provide cooling shade. Research work shows the benefits of using light colour roofs and roof gardens to minimize the heat gain inside the buildings. This would in turn reduce the energy consumption of air conditioning in buildings. Rooftops, balconies and terraces have often been referred to as the “last urban frontier” of cities, in the sense that they represent the last significant remaining space to be exploited to create a green oasis and an area of relative peace and privacy away from the urban bustle. Given the social, economic and environmental contributions of urban greenery to the quality of life in cities and the realization that cities more often than not lack sufficient greenery, greening of rooftops, balconies and terraces is therefore a logical, effective and high impact way to re-inject the much needed greenery in urban settings thus reinventing the concept of hanging gardens.

In order to provide for the establishment of an effective system for protection of plant varieties, plant breeders’ rights and the farmers’ rights and to encourage the development of new varieties of plants it has been considered necessary to put in place a legal protection mechanism the Protection of Plant Varieties and Farmers’ Rights Act, 2001 has been enacted in India (www.plantauthority. gov.in). For the purposes of this Act, Protection of Plant Varieties and Farmers’ Rights Authority has been established and is located at NASC Complex, DPS Marg, Opp - Todapur, New Delhi-110 012.

Origin, domestication and adaptation in India
Tomato was classified under Lycopersicon for several years although, initially Linnaeus (1753) classified it in the genus Solanum. Recently, molecular studies provided new data on the relationships of tomato-potato and based on these results, tomato has been again assigned to the genus Solanum. Solanum section Lycopersicon includes the cultivated tomato (Solanum lycopersicum) and its wild relatives, Solanum lycopersicum being the only domesticated species. The wild cherry tomato, Solanum lycopersicum var cerasiforme, the ancestor of the modern cultivated tomato has been originated in Peru-Ecuador-Bolivia region of the Andes. The Veracruz-Puebla region of Mexico is the centre of domestication of the cultivated tomato. From Mexico, tomato was taken to Italy, Spain, Portugal and other European countries, Africa and Middle East by the explorers in the 16th century. It was taken to Philippines after its discovery by Magellan in 1521, and from there it was taken to other Asian countries. Tomatoes did not have an easy passage even in Europe. The fruit got tagged as the “poison apple” in Britain, France, Spain and other European countries. Tomato moved to the USA from northern Europe around 1781 where it was first grown by Thomas Jefferson in Virginia.

Mass contact method in extension education involves reaching a large audience or community through various communication channels to disseminate information, raise awareness, and promote behavior change. Unlike individual or group contact methods that involve direct interaction with specific individuals or small groups, mass contact methods are employed to reach a broader audience efficiently. These methods leverage mass media, such as radio, television, newspapers, social media, and public events, to deliver educational messages and engage with the target population.

Objectives

1. Dissemination of Information: To provide accurate and relevant information on agricultural practices, technologies, health, education, and other relevant topics to a wide audience.

2. Awareness Building: To raise awareness about important issues, challenges, and opportunities within the community, encouraging informed decision-making and action.

3. Behavior Change Promotion: To promote positive behavioral changes, such as adopting improved agricultural practices, adopting healthy behaviors, and participating in community development initiatives.

4. Community Mobilization: To mobilize community members to participate in extension programs, community projects, and collective action for social and economic development.

5. Knowledge Sharing: To facilitate the sharing of knowledge, experiences, and best practices among community members, extension agents, and stakeholders.

The 15 CGIAR (Consultative Group on International Agricultural Research) Research Centers are independent, non-profit research organizations, conducting innovative research. Home to more than 8,000 scientists, researchers, technicians, and staff, CGIAR research works to create a better future for the world’s poor. Each Center has its own charter, board of trustees, director general, and staff. CGIAR Research Centers are responsible for hands-on research programmes and operations guided by policies and research directions set by the System Management Board.

The Cladocera, commonly known as ‘waterfleas’ form a primitive group of microcrustaceans. They play an important role in the aquatic food chain and also contribute significantly to the zooplankton dynamics and secondary productivity in freshwater ecosystem. Though most cladocera is filter feeders, they also feed on detritus, algae, small rotifers and copepod nauplii. They keep a check on eutrophication for which this group is also widely used as good indicators of ecosystem health and sentinels of environmental change (Eggermont and Martens, 2011; Padhye and Dahanukar 2015). At the same time, cladocera is the favorite food prey of many invertebrates and many vertebrates. Their characteristic jerky movements make them easily visible to the predators and that is why most of fish larvae were found to prefer cladocera over the prey (Mayer and Wahl,1971).

Cladocerans have a distinct head, a single compound eye, and a large mandible for grinding food particles. Cladocerans feed on algae, small rotifers, and copepod nauplii. Cladocerans reproduce mostly asexually via parthenogenesis. But they can also reproduce sexually based on the environmental conditions.

The distribution of cladocerans is affected by different factors including temperature, rainfall, water quality, nutrients, macrophytes, flood pulse etc. (Ghidini et al.,2009; Kiss et al., 2014). Their metabolic rate is variable with water temperature.

The order cladocera belongs to the subclass Branchipoda and includes minute crustaceans generally in the size range of 0.2 to 5.0 mm.