eBook Chapters

Summary

Tropical forests are declining sharply and continuously growing demand of wood leads to removal of trees from their natural habitats. Clonal propagation of superior trees is must for further exploration and conservation of these genetic resource. Tissue culture technique offers rapid and large scale propagation of desired traits. However, micropropagation of adult trees is difficult; many problems are associated like- maturity, contamination, phenolic leaching, season, variants, long life cycle, etc. Shoot bud culture is the easiest and successful tissue culture technique for the toughest material to clone- mature trees. The chapter deals with the bud culture method of heartwoods and associated problems at different stages – explant establishment, shoot multiplication, rooting and acclimatization. Also the possible solutions of the problems are discussed on the basis of previous reports.

Abstract

Quercus species of family Fagaceae are very important from both economic and ecological perspectives. These exhibit irregular fruiting, and produce seeds recalcitrant to storage, thereby difficult to propagate through conventional procedures. Again because of their very difficult to root nature, extremely limited success has been observed in vegetative propagation. Application of new technological approaches like in vitro propagation may be of interest for accelerating tree improvement programs to these hardwood species. This review provides a summary of the advancement in the application of in vitro propagation to oak species of Kumaun West Himalaya India.

A. In vitro Rumen Fermentation Technique for Forage Quality Evaluation

The term in vitro means “in a glass” in contrast to in vivo which is defined as “within the living body”. In vitro implies a close relationship to an in vivo measurement an in vitro reaction is used as a substitute for a reaction which normally occurs and is observed within a living body. the terms  “artificial rumen” and “in vitro rumen fermentation” are often used interchangeably.

The main advantage of the in vitro techniques rests in the ability to use them to study activity of micro-organisms away form the control and influence imposed by the host animal.

ABSTRACT

The in vitro culture studies in crop plants show that there is a differential response of various explants to different concentrations of auxins and cytokinins. This indicate that hormonal requirement is specific to the genotype and developmental stage of the explant. Therefore standardisation of media and explants were done for in vitro callus culture and plant regeneration in L. esculentum and L. pimpinellifolium at the Department of Olericulture, College of Horticulture during 2000-2002.

The source materials selected for the study were L. esculentum var. Sakthi and L. pimpinellifolium. The explants were leaf segments (0.5 cm²) from fully opened leaves, nodal segments (0.5 cm) and internodal segments (1.0 cm). The media consisted of the basal MS and ½ MS media along with different auxins and cytokinins either singly or in combination. The auxins used were NAA (0, 1, 2 and 3 mg l^-1 and 2,4-D (0.5, 1, 1.5 and 2 mg l^-1) and the cytokinins used were BA and kinetin (1, 2, 3, 4 and 5 mg l^-1).

ABSTRACT

Advances in biotechnology, particularly methods for culturing plant cells and tissues, can provide new means for the commercial processing of even rare plants and the chemicals they produce. These new technologies will extend and enhance the usefulness of plants as renewable resources of valuable chemicals. Aristolochic acid is mainly found in the genus Aristolochia. Aristolochic acid has been shown to have anti-tumour activity and has been recommended for its activity against various effects of snakebite and its capacity to inhibit phospholipase A₂ or other enzymes. Considering the importance of medicinal use of A. bracteolata and A. indica, in the present study, they were selected for in vitro development and rapid multiplication of plant through tissue culture. The study was undertaken to enhance the in vitro production of Aristolochic acid in different tissue cultures. Cell suspension culture was established from friable calli of both the species in MS liquid medium. Bergmann plating technique was carried out for cell line selection for more production of Aristolochic acid. Primary transformation was carried out by Agrobacterium tumefaciens strain LBA4404 / pTOK233 with Gus and hpt genes to confirm the transgenic potential of A. bracteolata and A. indica. In order to enhance the production of aristolochic acid in in vitro culture condition, the physical, chemical and biological conditions were altered. Aristolochic acid was isolated, identified and purified from naturally growing plants as well as from tissue culture plants and all other culture staes

1. Introduction

Atalantia monophylla (L.) DC. is a small tree belonging to the family Rutaceae; resembles Citrus in general aspects and is considered a close wild relative of Citrus. The species is commonly called as wild lime, in Malayalam it is called as Kattunaragam and in Tamil Katuuelumichai. A. monophylla is used in Ayurveda, Folk and Siddha systems of medicine. It is used for the treatment of rheumatoid pain and glandular swelling (Sankaranarayan et al., 2010). The root is believed to be antispasmodic (Kiritikar and Basu, 1999), the leaves are often applied for itching and other skin complaints (Panda, 2004) and acridone alkaloids isolated from roots were reported to have anti-allergic activity (Chukaew et al., 2008). The chloroform extract of leaves has shown antiviral activity (Chansakaow et al., 1996). Besides its medicinal value A. monophylla leaves are used as flavoring agent, berries are used for making pickles, juice of berries are used for dyeing purpose and wood as timber. It is also useful as a rootstock for breeding new cultivars of Citrus Linn. (Guha Bakshi et al., 1999). This species has high medicinal value and the natural population is under threat due to habitat destruction, use of wood as firewood, collection of fruits for making pickles and harvesting of leaves, roots for medicinal purposes by the local communities. Hence there is a need for developing an appropriate conservation strategy for this species.

Before applying fungicides in field at large areas, it is required to be thoroughly investigated under laboratory conditions for its efficacy on pathogen, subjected. Basic methods of testing these chemicals against subjected pathogen, under laboratory conditions are described here.

1. Poisoned Food Technique

Principle

The principle involved in this technique is to poison the medium with fungicides and then allow a teat fungus to grow on such medium. The test may be conducted both on solid and liquid medium.

Principle

Orchid seeds are produced in large numbers but are exceedingly small, hence are also called “dust seeds” may be as small as 0.01 by 0.05 mm in dimension and weigh as little as 10-30 ug. Seedling development is generally a slow process in orchids, with the seedlings passing through an initial stage, termed the protocorm stage, prior to the production of distinct vegetative organs (leaves, roots, etc). The protocorms of terrestrial orchids are subterranean and entirely heterotrophic, with all of the nutritional needs of the orchid being supplied by the mycorrhizae (the symbiotic fungus). The length of the protocorm stage is variable, however, in many cases, months, or even years, elapse before leaves and roots are produced.

The combination of a small seed size and fungal symbiont requirement largely precludes the propagation via conventional seed propagation protocols. Accordingly, most of the early research on the development of orchid seed germination protocols focused on in vitro germination with the seeds and fungal symbiont being sown on the same petri plate. Successful protocols were developed for several species; however, the process was complicated by the diversity of fungal symbionts required (across orchid taxa) and the difficulty in isolating and culturing these fungi.

In the 1920’s the process of orchid seed propagation was revolutionized by Lewis Knudson with the demonstration that nutrient media that could be used as a substitute for fungal infection. The isolation and culture of multiple fungal strains was no longer required, and improvements in germination frequency, germination speed, and seedling vigor were generally observed. To date, successful asymbiotic germination techniques, termed ‘flasking’, have been developed for many epiphytic orchids, with several species being saved from extinction via the application of these techniques.

Aplant collector and conservationist faces many problems. These include adverse weather, limited availability of personnel, insufficient material because of poor growing season, general scarcity of the plant in the target region, grazing of the plant by animals, damage by pests or diseases or immaturity of the material. These problems are common to both seeds and vegetative propagules. The vegetatively propagated plant material face another unique problem related to excessive weight and bulk. The cost and inconvenience of transporting large amounts of material can be a limitation on the scope of a plant exploration mission. There is additional need to maintain selected clones in a disease-free condition for distribution to national programmes.

The knowledge and principles from interconnected disciplines of bioengineering, material science and life sciences are combined in tissue engineering with the aim of developing a construct that can wholly or partly restore/maintain/augment the function of a damaged tissue or organ. Tissue engineering construct generally comprises cells on a suitable scaffold. The ultimate functionality of a tissue engineering construct is mainly dependent on the cell behavior on scaffolds. The proliferation and cell viability on scaffolds varies from one cell type to another and from one species to another. So it is important to investigate individual cell-scaffold combinations.

The similarity in structure and composition to bone mineral, osteoconductive properties, ability to integrate with the bony tissue and absence of immune response makes hydroxyapatite [Ca10 (PO4)6(OH)2] the choice of calcium phosphate biomaterial with only disadvantage of slow rate of resorption. On contrary, silica–calcium phosphate composite in comparison to calcium phosphate is rich biomaterials having a faster resorption rate. Studies have shown that coating of hydroxyapatite with a calcium silicate containing layer encourages cell proliferation and osteogenic differentiation of human bone marrow-derived stromal cells.

In this chapter a composite scaffold (calcium silicate, hydroxyapatite and tricalcium phosphate), namely HASi with elements in the following percentages: 66.36 % - calcium, 25.35 % - phosphorus and silicon - 8.29 % and porosity of 50 – 500 µm is undertaken to evaluate the rBMSC proliferation and differentiation properties in vitro with the aim of assessing cytocompatibility of HASi and rBMSC in terms of cell attachment, cell morphology, cell proliferation and osteogenic differentiation.

The estimation of digestibility by feeding trials with sheep or other ruminants is laborious and requires large quantities of feed. Therefore, the chemical composition of feedstuffs are used to predict digestibility by making use of established mathematical relationship between digestibility and composition. However, since the relationship between digestibility and chemical composition varies with the type of feedstuffs, for each group of feedstuffs, mathematical equations will have to be developed by relating chemical composition with in vivo digestibility. In spite of this, the errors associated with this approach of predicting digestibility is large. This has led to the development of techniques that would mimic digestive function of the ruminant in the laboratory.

In vitro methods of feed evaluation using rumen fluid and/or digestive enzymes are the most widely used techniques in ruminant feed evaluation.Although,the technique adopted in different laboratories differ, the principle of the technique is essentially a partially simulated rumen environment in vitro by incubating rumen fluid anaerobically in buffered medium at 39°C for variable period of time. Currently the most commonly used rumen in vitro techniques are,

The Indian centre of origin and diversity of cultivated plants represent an outstandingly rich reservoir of nature’s wealth of plant species which include a large number of economically important species including Vegetable Crops. Common vegetables like okra and brinjal are native to this region. Out of the recorded 110 genera and 640 species of cucurbits, 100 species representing 36 genera occur in India. Twenty of these species are endemic. Adoption of high yielding crop varieties and hybrids along with changing land use patterns have led to rapid replacement of native cultivars in most cases. Conserving and sustainable utilization of locally adapted types and primitive landraces are, hence, now major concerns at the national and global levels.

The National Bureau of Plant Genetic Resources (NBPGR) at New Delhi is the nodal organization in India with the national mandate to plan, conduct, promote and coordinate all activities concerning plant exploration and collection, germ plasm import and exchange, plant quarantine, germplasm multiplication and distribution, evaluation and characterization, documentation and conservation of both indigenous and introduced genetic variability in cultivated plants and their wild relatives.

The challenging job of collecting, genetic evaluation and utilization of plant genetic resources cannot be satisfactorily accomplished by one organization. The enormous task of safeguarding and promoting greater utilization of these valuable resources can be best achieved through a networking approach. Professional development is the key to success of this endeavour. NBPGR organizes regularly short-term training courses on various aspects of PGR to impart latest knowledge and techniques to scientists and technicians engaged in crop improvement programmes and handling germpalsm collections.

Introduction

Vegetable crops include a large number of species, mainly used as an essential complement to the daily diet, providing vitamins, minerals, fiber, specific amino-acids and other active metabolites. Largely emphasizing the need to accumulate and conserve in gene banks the genetic diversity that is most useful to breeders. One of the primary reasons to sustain conservation of plant genetic resources in gene banks is to prevent the loss of genetic diversity. The great diversity of types in cultivation is also considered a genetic resource itself, to be maintained or increased, since it adds to the diversity of vegetables being grown and consumed and could serve to replace more established but similar crops in case of need (Crisp and Astley, 1985). The risk of genetic erosion due to the introduction of single new cultivars was considered especially high by Crisp and Astley (1985) for those vegetables that are built on a narrow genetic base, such as garlic, broccoli, tomato, cucumber, etc. Genetic erosion is difficult to document with solid data available in vegetable crops. On a global level, efforts of the International Board for Plant Genetic Resources (IBPGR, now IPGRI) to conserve vegetable crop germplasm began in 1980, with the definition of a number of priority crops for conservation, according to their importance for rural development and to their economic value for farmers in the tropics (Abelmoschus esculentus and related species, Allium spp., Amaranthus spp., Brassica spp., Capsicum spp., Cucurbita spp., Lycopersicon esculentum, Momordica charantia and related species, and Solanum melongena). During last six–seven decades, germplasm of promising and profitable varieties have been built up and developed by different agencies, initially assembling local types and selecting from them and by introducing promising types from other parts of the world. The introduction and acclimatization of vegetable germplasm from within the country and all over the world is important for exploitation and incorporation of useful characters such as early maturity, high yielding capacity, resistance to diseases and pests and tolerance to different climatic hazards, viz., drought, heat, cold and flood situations.

ABSTRACT

Dendrophthoe falcata (L.f.) Ettingsh, a unique hemiparasitic taxa belongs to the family Loranthaceae, spreads over the entire forest in the South West Bengal. It is generally stem hemiparasitic taxa, as it is found mostly on aerial part of the host plant due to lack of root system. It depend on the host plant for mainly water and to some extend carbon. It synthesizes its own food. This plant has haustoria instead of root, they penetrate their haustoria into the vascular bundle mainly in the xylem tissue of the host plants. This species has numerous medicinal values with a long history of its use in Chinese traditional medicines. The present work deals with the anti-diabetic, phytochemicals and silver nanoparticles properties of D. falcata. The responsible enzyme i.e. Alpha-amylase breaks the large starch molecules which produces free glucose and simultaneously increases the blood sugar level and as a result hyperglycemia occurs. This study also reveals that the plant’s leaves extracts have inhibitory activities on this key alpha amylase enzyme and apart from that aqueous extract of this plant contains some secondary metabolites which is very crucial data for drug preparation and any other research purposes. The maximum amount of silver nanoparticles is present in this plant which is valuable as a silver line in near future implications for different drugs preparations

26.1 Introduction

We have already discussed the basics of tissue culture techniques in Chapter 2. Plant tissue culture techniques in combination with recombinant-DNA technology are essential requirements for development of transgenic plants (will be discussed in next chapter). However, tissue culture techniques like anther, pollen, ovule and meristem culture can themselves be used for improvement of different traits or as an aid to conventional breeding methods. In recent years, isolated microspore culture has been preferred as a breeding tool and an experiment system for various genetic manipulations. Besides, using protoplast fusion and hybrid embryo rescue, distant hybridization has been successfully achieved in many crops. Protoplast fusion technique can be used for the transfer of cytoplasmic male sterility from one species to another in very short duration. Somaclonal variation generated during tissue culture can also be utilized for recovery of variants showing disease resistance or other desirable characters.

India over several millenniums has been the treasure land of biological wealth, intellectual knowledge and spiritual wisdom. During both pre and post Vedic periods, arts, science and technology had flourished well in various fields. Indian agriculture to some extent is much traditional and dates back to about 10,000 years. India with its traditional crop varieties and crop production technologies was able to feed its population and also exporting some selected produces to other continents. All the Indian villages had been the self sufficient, self sustained and self reliant units. The development of modern agricultural production was achieved by creating large scale specialized farm production units, increased mechanization and use of chemical inputs.

Family: Convolvulaceae

Flowering: Most part of the year

Fruiting: Most part of the year

Botanical description: The plant is a semi-aquatic tropical herbaceous plant. The stem of the plant is thick, fistular or spongy, rooting at the nodes trailing on moist soil or mud, or floating on water. Leaves are variable in shape and size,such as ovate-oblong, ovate- triangular, lanceolate, elliptic-oblong.The flowers are trumpet-shaped, 3-5 cm diameter, usually white in colour with a purple center.Capsule ovoid, glabrous and 2-4 seeded.

Common name    :    English- Scarlet morning glory         
        Hindi- Lalpungli
        Odia- Panikod

Family    :    Convolvulaceae
 
Habit & Distribution
The plant is (Fig. 53 a) a herbaceous climber, common in open forests and open places over hedges, bushes, fairly naturalized. It is naturalized throughout India.

General Characters
It is a twining, smooth to hairy annual plant. Leaves are ovate or broadly ovate, heart shaped having bases with pointed tips. The flowers are red in colour with a longer stalk than the leaves. Each flower stalk may bear a single flower or may have several flowers. The fruit is a round capsule up to 8 mm in diameter containing few seeds. Flowering and fruiting occurs during September-January.

Common name    :    English- Yellow-Throated Morning Glory     
        Hindi- Neelkalme
        Odia- Kaladana

Family    :    Convolvulaceae

Habit & Distribution
The plant is an ornamental climber (Fig. 54a), common in waste places and road sides. It is widely distributed throughout the tropical countries.

General Characters
It is a climber. Stems trail for several metres and climb over low bushes, reddish, muriculate, hairless or velvety. The Leaves are ovate, 5-15 × 5-15 cm, blunt or long-pointed at the tip and heart-shaped. Flowers are funnel-shaped, blue with purplish tinge, 3.5-4.5 cm long, with a yellow center and borne in several-flowered cymes. The cyme is carried on a stalk 5-25 cm long. Capsule is ovoid to globose, hairless. Seeds are trigonous, brownish, hairless or very shortly velvet-hairy.

Common name    :    English- Royal jasmin         
        Hindi- Chameli
        Odia- Niali/Banamali

Family    :    Oleaceae

Habit & Distribution 
It is a shrub (Fig. 55 a), commonly found in forests and distributed in Central and Southern India.

General Characters
The plant is nearly erect, not twining or climbing. Branchlets are hairy. Leaves are simple, opposite, variable in size up to 18 cm long and 10 cm broad with long pointed end. Flowers are white, fragrant, three forked clusters with flowers stalk of 5 mm long. Berry is mostly simple, 1-1.5 cm long, ellipsoid and black when ripe. The plant flowers during March-May and bears fruits during June-July.

Common name    :    English- Bellyache bush         
        Hindi- Ratanjoti 
        Odia- Baigoba/Baigewa

Family    :    Euphorbiaceae

Habit & Distribution
It is a shrub (Fig. 56 a), very commonly found in waste ground, village hedges, roadsides and scrub forests. The plant is cultivated and naturalized in several parts of India.

General Characters
The plant is a small dark colored glandular hairy shrub, 0.8-1.8 m in height. The leaves are 6-12 cm in diameter, palmatly 3-5 lobed, entire with 3-5 cm long petiole. Flowers are red with yellow centre. The petioles and stipules have glandular hairs. It flowers and bears fruits during July-October.

Common name    :    English- Peregrina/Spicy jatropha
     
Family    :    Euphorbiaceae

Habit & Distribution
The plant is a shrub, usually planted in gardens.

General Characters
It is a small erect and branched shrub (Fig. 57 a). The leaves are entire, elliptic-oval, may be fiddle shaped with 7 inches long. The flowers are red and pink and about 2.5 cm across and borne in many flowered clusters at branch end. Fruit is rounded-trigonous. The plant bears flowers and fruits in most parts of the year.

Family: Cucurbitaceae

Flowering: July-October

Fruiting: November-January

Botanical description: The plant is a vigorous, annual, climbing herb. It grows fast and may begin to flower only two months after seeding. The vine is branched and climbs by means of tendrils along the stem. The foliage is covered with soft hairs and has a foul musky odor when crushed. The leaves of the plant are up to 15 inches wide, circular in overall shape, with smooth margins, a few broad lobes, or with undulate margins. Leaves have a velvety texture because of the fine hairs, especially on the undersurface. The flowers are white in colour and borne singly on the axils of the leaves, the males on long peduncles and the females on short peduncles. Fruits are of various shapes like bottle or dumb bell-shaped, rounded or elongated.

Common name    :    Odia- Laoo
        English-Calbash     
        Hindi- Lauki

Family    :    Cucurbitaceae

Habit & Distribution
The plant is a climber (Fig. 58 a), commonly cultivated and distributed throughout India.

General Characters
It is an annual vigorous herb, running or climbing vine with large leaves and with lush appearance. Leaves are simple, 17-20 cm diameter, short and softly hairy with kidney or heart shaped outlines. Flowers are white, up to 4 inches in diameter with spreading petals. Fruits are of various shapes, in different varieties, like bottole or dumbbell shaped, rounded or elongated. The plant flowers during July-January and bears fruits during cold season.

Common name    :    English- Wild sage 
        Hindi- Raimuniya 
        Odia- Naguari

Family    :    Verbenaceae
 
Habit & Distribution 
It is a shrub (Fig. 59 a), commonly found in roadsides, wasteland, damp site etc. It is distributed throughout India.

General Characters
The plant is an evergreen shrub grows up to 6 ft height and may spread 8 ft in width. The leaves are ovate-oblong, about 2-5 inches long with rounded tooth edges and a textured surface. Stem and leaves surfaces are covered with rough hairs and emit an unpleasant aroma when crushed. Flowers usually white-pink or orange-yellow in colour and are held in clusters that are typically 1-2 inches across. Fruits are small, globose, fleshy, purplish black berries after ripe. The plant bears flowers and fruits in all round the year.

Family: Brassicaceae

Flowering: June-August

Fruiting: August-October

Botanical description: The plant is an annual herb that reaches at a height upto 2 ft, with many branches on the upper part. The leaves are entire, the upper ones are stalkless whereas the lower ones are stalked. Flowers are white to pinkish in color, small, 2 mm across, borne in long racemes. The fruits are small pods, obovate, with two seeds per pod. The seeds are brownish red in colour and become slimy when soaked in water. Garden cress is native to mediterranean region, introduced and naturalised, cultivated throughout the world. Within India, it is cultivated as a salad plant and found as an escape.

1. Botanical Name : Leptadenia pyrotechnica Forssk. Decne

2. Family : Apocynaceae

3. Common Name : Kheep, Broom bush

4. Name in Indian languages

Kheep (Hindi), Khip(Punjabi), Jivanti (Sanskrit).

5. Description

It is a much branched often leafless erect shrub. The edible part is tender fruit. It can grow up to 3 metres height. A desert plant, it is often utilized by local people within its native range, particularly as a fibre plant and for fuel. It is also planted in soil stabilization in arid areas.

6. Origin and distribution

It is originated from North Africa to desert areas to Western India. It is distributed throughout the arid zone of Rajasthan particularly in Jodhpur, Bikaner, Jaiselmer districts of Rajasthan. Kheep is also found gorwing in Pakistan, Iran, Arabia, Egypt, Sudan, Somalia, Chad, Libya and Algeria.

Antony Van Leeuwenhoek (1681) first observed the spirochetes under the microscope and described them. In 1833, Ehrenberg used the name Spirochaeta for free living spiral shaped organisms. Obermeir (1873) first described a spirochete of pathogenic significance isolated from human patients with relapsing fever. Inada (1916) first isolated Leptospira icterohaemorrhagiae from human patients of Weil’s disease. In 1980, Nogochi proposed the name as Leptospira.

In India, Taylor and Goyale (1931) first reported Leptospira from human patients suffering with jaundice in Andaman Islands. Ayyar (1932) first reported Leptospira from dogs in Chennai city.

Family: Lamiaceae

Flowering: July-October

Fruiting: October-January

Botanical description: The plant is an erect and diffusely branched annual herb, growing up to 20-60 cm in height. Leaves are linear or oblong, 2.5 to 7.5 cm long with blunt tips and scalloped margins. Whorls are large, terminal and axillary, about 2.5 cm in diameter and crowded with white bell shaped flowers. Calyx is variable, with an upper lip and short, triangular teeth. Nutlets are 2.5mm in size, smooth and brown.

Family: Plantaginaceae

Flowering: September-November

Fruiting: December-February

Botanical description: The plant is a perennial, small marsh herb. Stems are much branched, hairless. The aerial stems are up to 15 cm height, simple or branched, hairless, with stalked or stalkless glands. The submerged leaves are 1.5-2.5 cm in size, pinnately cut into segments which are flattened to capillary. The aerial leaves are usually whorled, pinnately lobed, 0.4-2 cm, sometimes few opposite and toothed. Flowers arise singly in the axils of aerial leaves. Flower stalk is slender, and 2-10 mm in length. Petals are botched with dark purple or reddish spots. The capsule is dark brown, compressed with ellipsoid to round in shape.

Family : Rutaceae

Flowering: February-April

Fruiting: November-January

Botanical Description: The plant is an erect, slow-growing tree with a few upward-reaching branches. The bark is ridged, fissured and scaly with sharp spines. The leaves are alternate, 3 to 5 in long, dark-green in colour, leathery, often minutely toothed, blunt or notched at the apex, are dotted with oil glands and slightly lemon-scented when crushed. Flowers are greenish or reddish in colour, 1/2in across, are borne in small, loose, terminal or lateral panicles. The tree is mostly known for its hard woody fruit, size of a tennis ball, round to oval in shape. The pulp is brown, odorous, resinous, acid or sweetish, with numerous small, white seeds scattered through it.

Murray (1926) and Pirie (1927) first described Listeria independently. In India, animal listeriosis in sheep was first reported by Mahajan (1936) in Andhra Pradesh.

Robert Koch first isolated Erysipelothrix in 1876 from a mouse he inoculated with putrefying blood. Koch initially named the bacterium “Bacillus of mouse septicemia”. Pasteur and Dumas (1882) first isolated Erysipelothrix from the infected pigs. Friedrich Loffler (1886) first experimentally produced the infection in pigs. Rosenbach (1909) first described human erysipeloid. The nomenclature of the bacterium is changed several times and Erysipelothrix rhusiopathiae was finally accepted by the scientific community in 1966. The name is originated from Greek words such as ‘erysipelas’ (red skin), ‘trix’ (hair), ‘rhusius’ (reddening) and ‘pathus’ (disease).

Common name    :    English- Angled luffa
        Hindi- Karvitori
        Odia- Pita taradi

Family    :    Cucurbitaceae

Habit & Distribution 
The plant is a climber (Fig. 60 a), widely cultivated and also found in wild. It is cultivated throughout India.

General Characters
It is an annual climber with smooth and angled stem. The leaves are 5-7 lobed or angled, 7.5-15 cm across and covered with short hairs. Flowers are yellow in colour with male and female in the same axil. The fruits are ribbed and cylindricaly shaped and 1-2 feet in length. The plant bears flowers and fruits during August-November.

1. Botanical name :Machilus edulis King ex Hook.

2. Family : Lauraceae

3. Common Name: Pumsi, wild avocado

4. Name in Indian languages : Pumsi, Phumkung (Lepcha), Lachyphal, Kawala (Nepali).

5. Description

Pumsi is an evergreen species grow up to a height of about 15-20 m, with spreading branches. It grows from Nepal to Sikkim, Bhutan, Arunachal Pradesh and the whole north-eastern region. Pumsi is considered as one of the forms of Avocado (Percea americana) in its native region.

6. Origin and distribution

It is native of India,Nepal, Bhutan, Sikkim, Arunachal Pradesh and the whole north-eastern region. The plants growwell incool humid regions in hills.The species is found in low density in forests at 1200 - 2400 metres height.The species is hermaphrodite, suitable for light sandy to loamy and clay soils. It can grow in semi-shade. It prefers moist soil.

Family : Sapotaceae

Flowering: February-April

Fruiting: May-July

Botanical Description: The plant is a large or moderate-sized tree, smooth and brown bark with pubescent or tomentose branchlets. The leaves are clustered near the ends of the branches, elliptic, oblong or ovate and acuminate. The flowers are cream coloured, fleshy, on rusty tomentose pedicel and clustered at the end of the usually leafless branches. The fruits are fleshy, green berries, quite large and containing from one to four shiny, brown seeds.

1. Botanical Name : Madhuca longifolia var. latifolia (Rox.) A. Chev.

2. Family : Sapotaceae

3. Common Name : Indian Butter Tree

4. Name in Indian languages

Mahua, Mohwa (Hindi), Maul (Bengali), Kat-Illipi (Marathi), Illupa Malayalam), Ippa (Telugu), Mahudo (Gujarati), Hippe Mara (Kannada).

5. Description

Indian Butter tree (mahua) is one of the most important trees of Indian forest. It produces delicious and nutritive flowers, fruits, seeds and valuable timber. It is a deciduous tree with a large, spreading, rounded crown that can grow 16 metres or more tall. The short main trunk can be up to 80 cm in diameter. Mahua is an indigenous tree of high economic value. A characteristic tree of the dry region, it is found in north and central India. The tree is very well known to rural folk since ages in India. Its flowers, fruits, and oil obtained from seeds are eaten in various ways. Mahua flowers are a rich source of sugar containing appreciable amount of vitamins and minerals. Fruit is eaten as raw or cooked. Pulp is a good source of sugar, whereas dry husk makes a good source of absolute alcohol. Seed is a good source of oil.

Common name    :    English- Mango
        Hindi- Aam
        Odia- Amba 
            
Family    :    Anacardiaceae

Habit & Distribution
It is a tree (Fig. 61 a) grows wild in valleys and widely cultivated throughout tropical India.

General Characters
The plant is a large, erect and ever green tree gowing up to 30-80 ft in height with a broad and rounded canopy. The leaves are variable in size and shape, usually lanceolate, entire, acute to acuminate with 10-20 cm in size. The flowers are greenish-yellow, small and polygamous with 5 lobed calyx which are ovate-oblong or elliptic in shape and remain spreading. There is a great variation in the size, colour and flavour of the fruits. They may be nearly round, oval, ovoid-oblong or somewhat kidney shaped and usually yellow in colour when ripe. The plant bears flowers during January-March and fruits during April-May.

Family : Sapotaceae

Flowering: November-January

Fruiting: March-June

Botanical Description: The plant is a tree growing up to 3-12 m tall, with gray bark and smooth branchlets. The leaves are alternately arranged often closely clustered towards the end of branchlets, obovate to obovate-elliptic leaf blade with 0.8-2 cm leaf-stalks. Flowers are white or light yellow in colour, about 4 mm in size and arise in fascicles in leaf axils. Berry is oblong to ellipsoid, 1- 2 seeded and yellow when ripe.

Family: Marsileaceae (Pteridophyta)

Flowering: November-January

Fruiting: January-March

Botanical description: The plant is a branched herbaceous plant with creeping rhizome and usually 3-15 cm or more in length. Leaves are composed of 4 obdeltoid leaflets. Leaflets are cruciform, obovate, variable in size depending on the ecological conditions, usually glabrous with rounded outer margin. Pedicels are 2-6, basal and slightly connate or free. Sporocarps are seen crowded or in groups of 2–3, arising at the base of the petiole.

Common name    :    English- Tiger’s claw/Devil’s claw
        Hindi- Ulat kanta
        Odia- Bagha nakhi

Family    :    Martyniaceae

Habit & Distribution
The plant is a herb (Fig. 62 a), commonly occur in roadsides, waste lands, and also naturalized. It is widely distributed in India.

General Characters
It is an erect, often branched herb that grows up to 2 m in height. Leaves are kidney shaped to circular, mostly 6-15 cm wide, both surfaces are equally hairy, margins with shallow lobes and leaf stalk is 9-14 cm long. Flowers are bell shaped purplish white with dark purple markings. Fruit is capsule, more or less ovoid, 2 valved, with the style splitting into 2 curved, lignified and spiny processes with curved claw of about 1 cm. The plant bears flowers and fruits during August-January.

Family: Lamiaceae

Flowering: August-September

Fruiting: September-October

Botanical dcescription: The plant is a glabrous perennial herb. Stems are erect, about 1 ft tall, usually less, purplish, hairless. Leaves are stalkless, sessile, lanceolate or ovate, acute with glabrous above and glandular below. Flowers are lilac, loose cylindrical, slender with interrupted spikes.

1. Botanical Name : Meyna laxiflora Robyns

2. Family : Rubiaceae

3. Common Name : Moina, Aliv

4. Name in Indian languages

Kutkura, Moin (Assamese), Mainphal, Muduna, Muyna, Muyuna (Bengal), Alu, Atu (Gujrati), Moina, Muduna, Muyuna (Hindi), Mullakare, Gundkare, gobergally (Kannada), Alu, Huloo, Halawni (Marathi), Pindi, Pinditaka, Pindituka, Pindu (Sanskirit), Manakkarai (Tamil), Segagadda, Veliki, Visikilamu (Telgu), Monono, Montaphoo (Odiya).

5. Description

It is a large common shrub or a small tree and commonly found in evergreen forests. Meyna is a genus of shrub and small trees distributed in tropical and subtropical region. The fruits are used for preparation of pickle.

Common name    :    English- Touch me not/sensitive plant
        Hindi- Lajwanti
        Odia- Lajakuli 
            
Family    :    Mimosaceae

Habit & Distribution
The plant is a common weed mostly found in roadsides, waste places, damp sites, agricultural lands etc. It is widely distributed throughout the better parts of India. (Fig. 63 a)

General Characters
It is a small prostrate or ascending herb with prickly stems and grows up to a height of 50 cm with a spread of 30 cm. The leaves are bipinnate, fern like and pale green, closing when disturbed. The flowers are pale pink or purple in colour, round to ovoid heads (8-10 mm in diameter) and the flower heads arise from the leaf axils. The plant bears flowers and fruits during August-May.

1. Botanical Name : Mimosops elengi L.

2. Family : Sapotaceae

3. Common Name : Spanish cherry, Molshri, Bullet wood tree

4. Name in Indian languages

Maulsri (Hindi), Magizhamboo (Tamil), Ilanni (Malayalam), Bakul (Bengali), Bakuli (Marathi), Ranjal (Kannada), Barsoli (Gujarati), Maulsari (Nepali).

5. Description

Maulshri is a evergreen small tree of the Indian subcontinent. It provides a dense shade. The main stem is often short and divided into several large main branches. The tree provides food, medicine and a range of commodities for the local people. It is often cultivated in the tropics and subtropics, as an ornamental avenue tree and in gardens.

Common name    :    English- Spanish cherry
        Hindi- Maulsari
        Odia- Baula
        
Family    :    Sapotaceae 

Habit & Distribution
The plant is a small tree (Fig. 64 a), frequently planted elsewhere also in gardens. It is distributed throughout India.

General Characters
It is a small tree with small shiny thick narrow, pointed leaves, straight trunk and spreading branches. The leaves are elliptic or oblong, 6.2-10 cm long. Flowers are small star shaped, white, yellowish white in color with a crown rising from the centre. The fragrance of flowers is very graciously scented. The fruit turns yellow-orange when ripens and are eaten fresh. The plant flowers during April-May and bears fruits during August-September.

Family: Rubiaceae

Flowering: September-December

Fruiting: December-March

Botanical description: The plant is erect or diffused, pubescent herb, growing up to 40 cm in height. The leaves are sessile, ovate-lanceolate, acute, pubescent and rounded at the base. Flowers are small, white in colour and verticillate in axillary fascicles with small calyx. Capsule is globose, less than 1 mm across with 2 minute seeds and having furrowed on one side.

Family: Cucurbitaceae

Flowering: June-October

Fruiting: October-February

Botanical description: Bitter gourd is a herbaceous, tendril-bearing, slender climber, growing up to 5 m. It bears simple, alternate leaves 4-12 cm across, with 3-7 deeply separated lobes. Each plant bears separate yellow male and female flowers, about 2-3 cm in diameter. Male flowers are more numerous, having a yellow center and conical base, while female flowers have a green center and a small bump at the base. The fruits are oblong shaped and tapering at both ends, 5-20 cm long and turns yellow when ripe. It is hollow in cross-section, with a relatively thin layer of flesh surrounding a central seed cavity filled with large flat seeds and pith. Seeds and pith appear white in unripe fruits.

Family : Cucurbitaceae

Flowering: August-September

Fruiting: September-November

Botanical Description: The plant is a large perennial climber which grows up to 3-5 m. The leaves are simple, alternately arranged, ovate and 3-5 lobed. The flowers are yellow, 4-6 cm across, solitary on axillary stalks. Fruit is an ellipsoid, 3-6 cm long, densely covered with soft spines.

1. Botanical Name : Morinda pubescens

2. Family : Rubiaceae

3. Common Name : Indian Mulberry, Morinda tree

4. Name in Indian languages

Aal (Hindi), Dhaula, Aseti (Marathi), Mannanunai, Mannanatti, Nonu, Nuna (Tamil), Mannappavitta (Malayalam), Maddi (Telugu), Haladipavette, Maddi (Kannada), Achu, Pindra (Oriya) , Paphanah (Sanskrit).

5. Description

Aall or Indian mulberry (Morinda pubescens) is a species of flowering plant in the family Rubiaceae, It is an evergreen shrub or small tree growing . The fruits are small and edible. A scarlet colouring matter is procured from the roots and bark, and used for dying hand kerchiefs, turbans, etc. It is also used to assist more expensive dyes in giving a red colour to yarnand cloth. The red threrads used in carpet-making used entirely to be dyed with it. The tree formerly used to be much more commonly cultivated than it is now. Since the introduction of the cheap aniline dyes’s its cultivation decreased.

Family: Moraginaceae

Flowering: January-March

Fruiting: April-June

Botanical description: The plant is a small deciduous tree, having corky gray bark, branching and fernlike leaves and growing up to 10 metre in height. The leaves are pinnately compound, 25-50 cm long with thickened and articulated at the base. The flowers of the plant are scented and white in colour with lanceolate petals. Capsule is pendulous, long bean like seed pods and usually 22-45 cm long.