eChapters

Antimicrobial Resistance abbreviated as AMR is defined as the process by which microorganisms like bacteria, fungi, viruses, and parasites change over a period and become resistant to a drug to which they were earlier susceptible to. This makes the medicines ineffective for treatment overtime, thus making infections difficult to treat thereby increasing the risk of spread of diseases caused by them and severe illness which may lead to death. This in turn necessitates the need for development of newer antibiotics. However, there has been a sharp decline in the development and manufacture of new antibiotics in the recent years resulting in development of only few antibiotics in the past two decades. The increase in emergence of antibiotic resistance concurrent with decline in development of newer antibiotics poses a serious threat to health of both humans and animals. Every year about 700,000 deaths have been reported to occur due to resistant infections and this rate is likely to increase further with estimated infections due to resistant pathogens expected to reach over 50 million globally by 2050 (O’Neill, 2016). The worrisome trends of increasing antimicrobial resistance across the globe poses a serious threat to both animal and human health as AMR not only increases the morbidity and mortality rates but also increases the economic burden on health care (Zhu et al., 2022). This presents an extremely grave situation as the supply of new antibiotics is inadequate to cope with the increase of AMR pathogens. The unnecessary use of antibiotics globally especially in the livestock sector as growth promoter further helps in selective enrichment of AMR pathogens which further increases health risks. It is well known that resistant pathogenic and commensal bacteria can persist and spread within and between premises despite zero antimicrobial drug use. This may be explained due to maintenance, replication, and further dissemination of resistance genes by horizontal transfer of genes via natural ecosystem and can occur either by conjugation, transformation and transduction; and these mechanisms can rapidly spread AMR genes among microbial community in natural environment and human microbiome, where these resistant bacterial species may be selected due to presence of antimicrobial drugs (Zhu et al., 2022). The physical transfer of bacteria via movement of animals, workers, and equipment and ineffective cleaning and disinfection may also be responsible for increasing the AMR pathogens in the natural environments.

AMR has been prevalent in almost all European countries from past decades. A 2021-2022 report published by the European Food Safety Authority (EFSA) and the European Centre for Disease Prevention and Control (ECDC) on AMR in zoonotic and indicator bacteria from humans, animals and food origin reported vast differences in antimicrobials resistance levels among the European countries, with comparatively higher AMR percentages in the southern and eastern parts of Europe (ECDC, 2022, 2023). The resistance levels ranged from very high for tetracyclines, sulfonamides and ampicillin, moderate for ciprofloxacin to low levels for third-generation cephalosporins (cefotaxime and ceftazidime) in human cases infected with Salmonella spp. in 2022 (ECDC, 2023). The trends are dynamic and over a decade of 2013 2022, the human isolates from 12 and 15 countries reported a statistically significant reduction in tetracycline and ampicillin resistance, particularly for S. typhimurium (ECDC, 2023). The ciprofloxacin and cefotaxime combination, a highest priority critically important antimicrobials (CIA), had very low and rare resistance levels for Salmonella isolates of humans and animal and their derived meat.

Livestock species wise variation was also observed in the resistance pattern in imported meat, being higher in poultry meat isolates compared to pig or cattle meat for Escherichia coli (ECDC, 2023). High to extremely high levels of f luoroquinolone resistance from Campylobacter jejuni and C. coli of both human and animal origin isolates exist, due to which the fluoroquinolones can no more be used as a treatment option for human Campylobacter infections (ECDC 2023). The combined occurrence of erythromycin resistance and ciprofloxacin in Campylobacter spp is considered of high public health importance, and the report mentioned that the combined resistance for these antimicrobials was higher in C. coli compared to C. jejuni from food-producing animals and humans. However, while the ciprofloxacin resistance showed an increase in over 13 study countries, seven countries reported reduced erythromycin resistance for C. jejuni. Overall, resistance to conventional antimicrobials was frequent in Campylobacter and Salmonella from humans and animals. Increasing trend in resistance to one of two CIA for treatment in humans has been observed in poultry-originated Salmonella serovars and Campylobacter while combined resistance was is low in few Salmonella serovars and C. coli isolates from humans and animals in some countries (ECDC, 2023).

Introduction to Antibiotic Discovery

Antibiotics are the drugs that are used to treat illnesses caused by bacteria in humans and animals. These act by killing or inhibiting the growth and multiplication of bacteria. Antibiotics were considered as “magic bullets” that selectively kills microbes responsible for disease causation, but at the same time would not affect the host (Zaman et al., 2017).

Louis Pasteur in 1877, first discovered the properties of antibacterial compounds with the inhibition of anthrax by saprophytic bacteria. In 1928, Alexander Fleming observed that the growth of Staphylococcus aureus in petri dishes was inhibited by substances produced by the fungus Penicillium chrysogenum, which led to the discovery of the first antibiotic, penicillin (Aminov, 2017). Selman Waksman, discovered multiple antibiotics from filamentous actinomycetes living in the soil and defined an antibiotic as “a compound made by a microbe to destroy other microbes”. Following the discovery of Penicillin, various antimicrobial compounds such as sulphonamides, aminoglycosides, tetracyclines, lipopeptides, oxazolidinones, glycopeptides, streptogramins, and quinolones were discovered in the “Golden era” of antibiotics during the period from the 1940s to the 1960s and most of the antibiotics still in current use were discovered during that period (Salam et al., 2023). Millions of metric tons of newer classes of antibiotics have been produced in last 60 years since its inception.

Scheduled Caste Sub Plan (SCSP) and Tribal Sub Plan (TSP) were initiated by the government to guarantee funds to SC/STs both at State and Centre in pro portion to the size of their respective population. It was in 1974-75 in the Fifth Five Year Plan period that the Government of India introduced the policy of the Tribal Sub-Plan (TSP) and later in 1979-80 in the Sixth Five Year Plan period the Special Component plan (SCP) which was renamed as Scheduled Caste Sub Plan in April, 2006 and renamed as Allocation for the Welfare of Scheduled Caste (AWSC) in February, 2017.Specifically, the SCSP and TSP mandated that pub lic resources towards SC/ ST welfare must be earmarked in proportion to their share in total population.The Narendra Jadhav Committee was constituted by the Planning Commission in 2010 in this regard.Every year, the Union Budget makes allocations exclusively for the Scheduled Caste and Scheduled Tribe.This fund is spent through the Scheduled Caste Sub Plan (SCSP) and the Tribal Sub Plan (TSP).As per the recommendations of the committee the earmarking of funds for all Central Ministries/Departments taken together should be at least 16.2% and 8.2% of the total Plan outlay respectivel under the SCSP and the TSP. The aim of such dedicated allocation is to promote nutritional security, livelihood security and entrepreneurship among these communities.

Stride of ICAR-IIVR and Operational Are

Amid nutritional and livelihood security of tribal population ICAR- Indian Insti tute of Vegetable Research (ICAR-IIVR), Varanasi has adopted 2724 tribal house holds from 24 villages of six clusters namely Faripan, Manvasa, Bakuliya, Chet wa, Jugail, Dahakudandi of Sonbhadra district of Uttar Pradesh and one cluster from Ranchi, Jharkhand for implementation of TSP programme of the institute. Similarly 2566 Scheduled Caste (SC) households have been adopted from 33 vil lages of five different clusters of Varanasi, Mirzapur and Chandauli districts for implementing SCSP programme.

Introduction

India is known to possess a significant demographic dividend due to its large and growing population where the female population is estimated to be 586.5 million or above currently. This population is characterized by vast regional and cultural differences that create difference in terms of region and culture due to which any kind of generalization is not possible. Women play a crucial role in the growth of the economy and over the years, they had made a substantial impact and achieved status across different sectors both within the country and overseas. Women is a part of workforce but still there is a large population of women both in rural and urban areas that has dreams, but lack the opportunities to realize those dreams and take up their roles other than the household ones. In this regard, it is essential for to promote skill development and women empowerment. In order to upgrade employability and other skills among women, there is need to reduce the unemployment, demographic dividend, cultural and regional barriers and bridge the gap between talent and industry by various innovative methods.

Proactive involvement of society is essential to attain the sustainable development and bring the desired change in the society. Women in economically disadvantaged areas lack opportunities for employment and for self-employment due to various causes and sometimes women in need are on the look-out for affordable vocational courses that is not only of interest to them but also enables them to enhance their employability. Women from these areas can be equipped with the skills they need to increase their employability so that they can access meaningful and sustainable employment, and self employment through entrepreneurship. UNICEF is also assisting women to build stronger links with the private sectors so that they have sustainable market access for their artisanal goods including those who are running home based business.

Introduction

Silk also known as the “Golden Fibre” is admired all over the world for its lustre, strength and smoothness. Sericulture industry is an ecofriendly agro based activity of mulberry cultivation, silkworm rearing and production. It is a labour intensive, rural cottage industry providing subsidiary employment and supplementing the income of semi-literate and semi-skilled poor workers throughout the year [Chanotraet al., 2019]. The term ‘sericulture’ includes economic activities which receive poor workers in the value addition at each stage like (i) raising plants for feeding the silk-worms (in the form of the mulberry plantation); (ii) production of disease-free silkworm layings (dfls), eggs, or seeds; (iii) indoor rearing of silkworms till the cocoon stage which produces silk (about 30-day egg-to-cocoon cycle), and (iv) sale of silk cocoons produced.

Silk industry can be broadly classified into; (a) sericulture, (b) post-cocoon technology – reeling (yarn production), spinning and twisting of silk yarn from the cocoons; and (c) weaving, fabric production, printing and dyeing, finishing, garment designing, marketing etc. These activities also provide the auxiliary enterprises of by-product utilization and machine manufacture including spun silk yarn manufacture and pupae oil extraction, thus creating livelihood for the small and marginal farmers and labour force.

Asia is the key producer of silk in the world producing over 98.5 per cent of the global output. There are more than 40 silk producing countries, but the majority of its production comes from China and India, followed by Brazil, Uzbekistan, Thailand, Vietnam, Korea and Japan. Unlike temperate countries, India has a distinct advantage of practising sericulture all around the year, as a result of its tropical climate, yielding a stream of about 4-6 crops (Savithri and Sujathamma, 2019).

Introduction

India has been advancing excessively in terms of workforce engagement as a lot of women have started seeking space in the business arena. The new age has given way to a new trend of entrepreneurshipwhich is flourishing without the quantification of gender. Needless to say, India has been dealing with a very bad history of patriarchal class arrogance and work division that cannot fully provide all the freedom to women who want to prosper in their professional lives.

The female entrepreneurs have to undergo a lot of obstacles right from the initiation of the idea till the plan operates. Sometimes, the existence as a women itself comes with a baggage of responsibilities. Indian women are bound to fulfill their responsibilities towards their family, community and work, balancing everything equally and in an efficient manner.

The conventionalism, ethnicity, social and cultural principles, parenthood, the tag of feeblest sex and insecurity curb the confidence of women in comparison to their male counterparts. Even though there have been considerable changes found in the women dwelling in urban areas, the rural women still have numerous hindrances blocking their path. The perceptive of the opponent sex is irrational in rural areas due to the lack of education and it becomes hard for the women to resist men in such cases.

The Indian customary draws a thin line between the capability and responsibility allotted to different gendersMohsen B., and Zouari S. (2014). It has even threatened the employment chances of females in the traditional and eccentric sphere of fiscal exercises. The present scenario has not broken the undeterred confidence of women who sought to make a career in business, since the last decade. With the alteration in technology, globalization and rivalry accentuation has made the operation of business more multifaceted and vibrant.

Introduction

India is bestowed with a lot of potentials to produce a variety of organic products due to its diversified agro-climatic regions and this is very true in case of Dehradun district of Uttarakhand. It offers a variety of geographic locations suitable for cultivating various food crops such as: fruits, vegetables, food grains etc. Globally, organic farming is cultivated in an area of 57.80 million ha. However, India accounts for just 2.59% of the area. The organic farming is followed in India in almost all the regions with three states Madhya Pradesh (34.34%), Maharashtra (13.19%) and Rajasthan (11.67%) accounting for about 59% of the total organic area of the country. The cultivated land under certification has substantially increased from 1.74 lakh ha in the year 2005-06 to 1.78 million ha in the year 2017-188 (https://www.researchgate. net/publication/338209816_Impact_of_farmer_producer_organization_on_ organic_chilli_production_in_Telangana_India) Agriculture and its allied sectors are the largest source of livelihoods in India. However, India still has many growing concerns. As the Indian economy has diversified and grown, agriculture’s contribution to GDP has steadily declined from 1951 to 2011. (Source: http://www.fao.org/india/fao-in-india/india-at-a-glance/). One of the main reasons is due to the lack of competence in Indian farmers for selling the crop in remunerative prices in the market. Some of the critical constraints and bottlenecks are lack of infrastructure for storage, transportation, quality control, packaging, price risk management, cool chains, market led extension, and framework for promotion of contract farming (Gohain, 2018). Realizing these issues Government of India under the Department of Agriculture and Cooperation identified Farmer Producer Organization (FPO) as the most appropriate institutional form to mobilize farmers and build their capacity to collectively leverage their production and marketing strength(Ministry of Agriculture, 2013).For analyzing the potential, importance and contribution of FPO in the agriculture sector, the present study aims to discuss the role of FPO in uplifting small and marginal farmers and its impact on the basis of the project undertaken by Silgur Bizat SwayattSahkarita FPO in Dehradun district of Uttarakhand.

Introduction

Novel Corona Virus 2019 (COVID 19) has impacted the human race very badly than any other disease in twenty first century. Almost every country on this planet was affected by this virus. The condition is more severe in developed countries.

So when it All Started?

There were reports that the medical professionals of Wuhan in China informed the government that there is some mysterious pneumonia like disease which is spreading very fast and affected the doctors and other paramedical staff who are attending the patients. When the situation became serious it was declared as outbreak of epidemic and first case of COVID 19 was declared in Wuhan China on 31st December 2019. Wuhan is one of the main business centres of china and there was regular movement of domestic and international visitors to that place. Till the problem was identified and communicated to other agencies, the spread of virus was already there in other parts of china and world specially Europe and USA.

World Health Organisation (WHO) declared COVID 19 as pandemic on 11 February 2020. Countries like USA criticized WHO for delay in identifying the disease and declaring it a pandemic.The damage was already done due to its spread in other countries. In India first reported case of COVID 19 was on 30th January at Kerala. However, considering the possible danger of this disease, Indian Government took primitive measure to contain the spread of Virus and announced Nationwide Lockdown 1.0 on 23rdMarch 2020. Similarly, Lockdown 2.0, 3.0, 4.0 were announced on 14 April, 3rd May and 17th may respectively.

Introduction

In view of the present Indian agriculture scenario, farmer producers organisations (FPOs) are emerged as a most appropriate institutional form to mobilize small and marginal farmers ,improved their access to technology, inputs and markets. As per the report of National Council on Farmers, these organisations are mainly promoted to combine the advantages of decentralized production and centralized services, post harvest management, value addition and marketing. (NCF,2004). In order to meet the dream of Atmanirbhar Bharat and to make farmers atmanirbhar, Farmer Producers’ Organizations (FPOs) is an appropriate form to mobilize them. Recently Government of India has announced a budgetary provision of 4496.00 crore for five years for promotion of 10,000 FPOs.(Cabinet Committee on Economic Affairs,CCEA2020)

Also to improve agricultural productivity and income of the farmers, it is necessary to develop an entrepreneurial culture among the member farmers of an organisation. To make this organisational approach more successful, member farmers need to develop planning ability, decision making abilitiy, managerial qualities ,scientific attitude, organisational skills, participatory skills etc. The member farmers as an agripreneur should possess knowledge about improved production techniques, markets, consumer preferences , schemes, credit availability, policy advocacy to enterprise effectively under FPO.

Introduction

Agriculture is one of the important pillars of the Indian economy. According to a report from FICCI, about 54 percent of Indian population depends directly on agriculture and it accounts for around 17.3 percent of GDP (FICCI, 2018). In the last two decades, powered by the Government policies and strong engagement of the industry and institutions, agriculture is rapidly evolving into agribusiness in terms of approach and structure. Transformation of Agriculture to Agri-business is one of the important strategies where enterprising farmers practice profitable agriculture.

India has become the third largest startup ecosystem hub. India is home to highest number of unicorn startups after US and China with 26 unicorns out of 250 plus total unicorns globally. At a time where with the increasing population and demand for better quality and higher quantity of food. India currently has more than 49,000 plus startups, 1500 plus investors, 250 plus incubators, and 26 unicorns (NASSCOM, Srivastav, 2018). The Indian startup ecosystem is ranked at number 37 and the global ranking of its top three cities are Bengaluru, New Delhi and Mumbai for the year 2018. In 2020, Bengaluru again continued its title “silicon valley of India” (Spews, 2021).

In a bid to double the farmer’s income by 2022, the Government of India is continuously looking for ways to boost agricultural production, food processing and marketing avenues through the integration of latest technologies and innovations; thus creating a huge scope for food and agritech startups in the country (Balaji, 2018). Many agritech startups in India are mainly in marketplace segment where e-commerce companies provide fresh and organic fruits and vegetables procured directly from farmers. Very recently many startups have come up providing innovative and sustainable solutions for farmer’s problems. Startups have provided solutions such as biogas plants, solar powered cold storage, fencing and water pumping, weather prediction, spraying machines, seed drills, vertical farming, etc (Sachitanand, 2018). EM3 Agriservices offers farming services and machinery rentals to farmers on a pay-for-use basis.

Introduction

Beekeeping is an environment friendly and agro-forestry based occupation that has established itself as an income generating activity and a commercial enterprise which not only promote agricultural and horticultural development but also contributes in upliftment of the rural economy of the country. Apiculture provides enormous potential for income generation, poverty alleviation and sustainable use of forest resources. Traditional beekeeping with Apisceranais widely practised by rural people in Uttarakhand who have inherited the tradition from their forefathers and maintain it till this day. The people in Uttarakhand have indigenous knowledge of beekeeping which is passed from one generation to another. In the hill area traditional hives are more suitable than modern hives. Modern beekeeping is infancy in the area, so people need to be encouraged to use modern bee hives for better management of colonies.

Uttarakhand is a land of variety of flora and fauna. Beekeeping forms an integral part of the small holder farming system and plays a significant role as a source of additional cash income in subsistence farming. There are around 2,50,000 beekeeping units in India out of which only 8,700 are in Uttarakhand accounting for about 2500 MT of honey production in 2016-17. Uttarakhand is extremely rich in Bee forage plants but the use of this rich resource is not being made properly (Tiwari, et al. 2010). Around 20.00 per cent of the Beekeepers in Uttarakhand do not use any medicine for the management of pest and diseases showing that the farmers are not much aware of the advance methods of beekeeping (Khan et al., 2007). Uttarakhand has huge potential for beekeeping due to plenty of flora and suitability of climate. Beekeeping is an ancient practice that is managed traditionally in Kumaon hills of Uttarakhand. Due to the traditional production system the productivity per hive is low in Uttarakhand. Therefore, it is Important to know about the constraintsfaced by the Beekeepers in practicing apiculture in Kumaon Hills of Uttarakhand.

1. Introduction

Milk is the principal source of protein and lactose in the diet. Dairy farming is viewed as a whole ecosystem of reciprocal relationships and reliance, as well as there production and safe guarding of societal values. Dairy farms are primarily made up of high-yielding dairy cows. Goats, sheep, and camels are some of the other animals employed in commercial dairy production. Milk has always been regarded as a healthy food. Milk includes more than9 other essential elements, including protein, carbs,vitamins, minerals, and lipids, in addition to calcium, which makes it critical for your bones.

Every year, about 600 million tonnes of milk are produced by over 264million dairy cows across the world. The global average for milk output per cow is around 2,200 litres. India is the world’s top milk producer followed by the United States, China,Pakistan, and Brazil. The economy of India is mostly dependent on agriculture and animal husbandry, with milk production playing a significant role. It is also the world’s largest producer of buffalo milk (FAO, 2019).

Co-operative dairy sector proved to be resilient as it continued to procuremilk from farmers and supply it to the consumers despite several logistical and other challenges. Milk production in India did not show any sign of slowdown. Milk productionis anticipated to be around 209.96 million tonnes in 2020-21, an increase of about 6.3 percent over the previous year. The estimated per capita availability of milk is expected to increase to about 425 gms /day which is more than the world average of around 315gms/day(NDDB,2020).The dairy co-operatives had a difficult year due to an influx of more milk production, a reduction in milk sales, and the building of inventories of preserved goods. Throughout the year, milk procurement by dairy co-operatives increased as it accepted diverted extra milk from farmers that would otherwise be sold to private and unorganized businesses. During the 2020-21 budget year, milk procurement by dairy co-operativesincreased by 7.9 per cent. Indian dairy co operatives have installed milk processing capacity of 66.3million litters per day,73.3million liters/day in private sector enterprises, and 2.5 million litres per day in producer companies.

Introduction

Animal husbandry and poultry rearing, in addition to offering economic participation, play an important role in securing household food security. The core notion of food security argues that everyone should have physical and economic access to the basic nutrition they require. ‘Food security is a scenario that exists when all people have physical, social, and economic access to sufficient, secure, and nutritious food that fits their dietary needs and food preferences for an active and healthy life’, according to the FAO (2002). Consequently, two requirements must be met in order to ensure food security: the first is the availability of food, and the second is the ability to purchase it. There is a propensity to define food security at the national level without examining its condition at the grass roots level with major emphasis at the household or individual level. This is because, while national food security may appear to be adequate, disparities in food security may exist among regions, groups, households, and people.

Increased crop output can increase people’s calorific intake, but protein is also essential for health, and pulses and coarse grains are essential. According to the Directorate of Economics and Statistics, DAC & FW, total pulse output in the nation was roughly 25.42 million tonnes in 2017-18, and over 23.40 million tonnes in the following fiscal year (2018-19). This demonstrates the country’s falling pulse production pattern, diminishing the availability of vegetable protein to the public. A similar trend was observed in states like Assam, where the area under pulse production declined from 1,54,706 hectares in 2017-18 to 1,50,229 hectares in 2018-19, and further fell to 1,43,962 hectares in 2019-20. The provisional data further forecasted the area to fall even further to 1,42,303 hectares in 2020-21. (Statistical Handbook of Assam, 2020 and 2021)

Introduction

NITI Policy Paper No.1/20017 reveals details of the concept and time frame as how to double the income of the farmers. The policy paper emphasizes in increase in agriculture productivity, improvement in total factor productivity, diversification towards high value crop, and increase in crop intensity, increasing terms of trade for farmers and shifting cultivators to non-farm and subsidiary activities. The well thought and planned program for doubling the income of the farmers reflecting dream or reality. We have farmers of different types like, small. Marginal and large farmers, resource rich and resource poor farmers, farmers of irrigated and non-irrigated zones, tribal and non-tribal farmers , trained and untrained farmers and progressive and non-progressive farmers. The farmers and their economic structure are quite different from place to place even within district. NSSO data on consumption expenditure survey (2011-12) reveal that more than one-fifth of households with self employment in agriculture and their principal occupation were having income less the poverty line.

Milk Production Scenario

Milk is primary source of protein and lactoseDairy farming is considered to be a whole environment of reciprocal relations and dependency reproduction and protection of social values. In worldwise dairy cows count up to 264 millions producing 600 million tones of milk every year. The global average for milk production is approximately 2200 litters/cow. Cattle population of world is 1009.69 millions heads (FAO, 2019) and highest milk producing countries are India, USA, China, Pakistan and Brazil. In case of consumption of milk India tops in the world followed by EU, USA and China. The per capita consumption of milk is highest in the country of Belarus followed by Ukraine and New Zealand. India is ranked 7th in the world in this respect. In India total cattle population is 306.70 millions. The state of Odisha constitutes 1.19% of the total cattle population of India. The level of milk production of India is reported to be 198.2 metric million tones. The per capita availability of milk in India is 427 gms/day (2021) and highest being in Punjab followed by Rajasthan. In case of Odisha the per capita milk availability is 143 gms and in milk production the district Cuttack top the list followed by Ganjam and Jagatsinghpur.

Introduction

India is the largest producer, consumer and exporter of turmeric in the world. Indian turmeric is considered to be the best in the world market because of its high curcumin content. India accounts for about 80 per cent of world turmeric production and 60 per cent of world exports. Major turmeric exporting countries are India, Thailand, Taiwan, and several other Southeast Asian, Central and Latin American countries. The major turmeric importing countries include Japan, Sri Lanka, Iran, UAE, US, UK and Ethiopia.

Turmeric is native of India and china. The word turmeric is derived from the French word ‘Terre-merite’ meaning merit of the earth. Its botanical name is Curcuma longa L. and belongs to family Zingeberaceae, it is also known as ‘Indian Saffron’ is an herbaceous perennial plant growing up to the height of 60 – 90 cm with short stem. The plant is propagated from rhizomes. The leaves are long, broad lanceolate and bright green. The flowers are pale yellow and born on dense spikes. The pseudo stem are shorter than leaves. The rhizomes were ready for harvesting about 7 to 9 months after planting.

India is popularly known as the “Spiced Bowl of the World” as a wide variety of spices with premium quality grown in a country since ancient times. Indian turmeric is considered to be the best in the world due to presence of high curcumine content. Turmeric contains up to 5.00 per cent essential oil and up to 5.00 per cent curcumine, a polyphenol.

Introduction

India is the second-largest producer of vegetables in the world after China. India accounts for 16 percent of the world’s production of vegetables. India home to 17.74% of the world’s population has only accounted for about 2.4% of the world’s geographical area and 4% of its water resources. Vegetables play an imperative role in nutritional security, economic viability and fit well into the predominant intensive cropping systems prevailing in different parts of our country. More than 60 kinds of vegetables are grown in India in tropical, subtropical and temperate agro-climates. India is the largest producer of okra and ranks 2nd in the production of potato, onion, cauliflower, and cabbage. During 2013-2014, India produced 162.19 million tonnes of vegetables and vegetable export from our country was worth Rs. 5462.93 crores. For a balanced diet, an adult who works moderately needs about 300 grams of vegetables per day, of which about 50 grams should comprise root vegetables, 50 grams of leafy vegetables and 200 grams of other kinds of vegetables as per Dietary Guidelines for Indians, NIN, 2011.

The vegetable is one of the important crops of Jharkhand, which is being cultivated on a commercial scale. About 3.2% of the gross cropped area of the State is devoted to vegetable crops. Vegetable development depends not only on production but also on the marketing system. Vegetable cultivation being labour intensive can substantially increase employment and livelihood avenues too. Vegetable growers face many challenges in the course of marketing their products and their marketing behaviour varies under different circumstances. Keeping this in view, the present investigation entitled “Marketing Behaviour of vegetable growers in East Singhbhum district of Jharkhand” was undertaken.

Introduction

Humans primarily get their nourishment from agriculture. According to the most recent UN estimates, the population of the globe could increase to over 9.7 billion in 2050, and to fulfill the demands of an expanding population and a rising need for food, the energy demand in the agricultural sector has dramatically expanded. However, a farm that produces food needs a steady supply of energy to run the machinery, irrigation pumps, and other devices that are often powered by fossil fuels. Because of the ongoing demand for energy, there is growing concern that these fossil fuels will also run out soon (Aroonsrimorakotet al., 2020). Additionally, other global issues like climate change, water shortage, and energy security are just a few variables affecting agricultural production that require more environmentally friendly and sustainable farming methods As a result, it is crucial to maximize land usage and increase agricultural production through the deployment of cutting-edge farming techniques and new technology for both economic effectiveness and the best possible use of the limited resources available in the form of land. There are many distinct uses for renewable energy in agricultural systems. Renewable energy sources can also be used to generate electricity for a range of agricultural tasks (Torshizi and Mighani, 2017). Renewable energy can come from a variety of sources, but solar energy is the ideal option because it can be installed practically anywhere on the field, is pollution-free, and is also the most economical. Unlike the usage of fossil fuels, using solar energy doesn’t release greenhouse gases. For remote locations with erratic access to electricity, it is the best renewable energy choice. Applications for space and water heating, greenhouse heating, solar photovoltaic systems, and water pumps for irrigation using various forms of solar energy technology (Mekhilefet al., 2013). There are numerous benefits of using solar energy technologies, such as solar panel water pumping systems, aside from being free of fuel costs, solar panels also have lower maintenance costs and require fewer replacement components than generators powered by diesel engines. In order to meet the growing demand for agricultural products from the expanding population, agriculture is a sector that aspires to accept the usage of solar energy and reaps enormous benefits from it. Therefore, solar energy technology has emerged as a solution in order to boost economic benefits to farmers and improve the environment by reducing CO2 emissions into the atmosphere, thereby addressing the issues caused by a growing population and a shrinking amount of available resources (Aroonsrimorakot and Laiphrakpam, 2019). The potential for farmers to move from using fossil fuels to using renewable alternative energy, which might lead to produce superior in both quantity and quality of food produced in various kinds of agricultural farms (Bardi et al., 2013). The issue will get worse unless the farmers change their perception about the adoption of solar energy technologies. Therefore, it is advised for farmers to employ energy-saving tools for producing crops and to maximize land by installing solar energy systems because they are practical, affordable, and useful for a variety of tasks (Duprazet al., 2011). Since many people still solely rely on fossil fuels for energy and lack access to modern energy sources, in light of this, the current study was done to assess the level of awareness and adoption of solar energy in agricultural operations.

Introduction

Women play a pivotal role in agricultural and rural economies in all developing countries. Rural India woman is facing Poverty and unemployment is the major problems. In India at the end of ninth five year span 26.1% of the population was living below poverty line. In the rural area 27.1% of the population was living under poverty. The overall unemployment rate is estimated to 7.32%. The female unemployment rate is 8.5%. The rate of growth of women unemployment in the rural area is 9.8%. A series of development programmes have been implemented for the development of economy in general ignoring women who constitute 50 percent of the total population. Role of women in development is an indispensable factor. Development programmes no longer can achieve their target without the participation and contribution of women. Women empowerment is multifaceted concept that extends to the psychological, economic, social, cultural, political and institutional spheres of women’s lives. The Government of India has launched and implemented a number of schemes towards poverty alleviation and women empowerment but it was observed that women in rural areas especially from the poor families could not be benefitted. Women empowerment is the most important instrument for the socio-economic development of a nation. In this context, SHGs have emerged as the tools that wield power to create a socio-economic revolution in the rural areas of our country. The empowerment of rural women is a key priority in the National Development Policy of India. In India, the organization of SHGs, especially those promoted by Non Governmental organizations (NGOs) is widely recognized as a development strategy for poverty reduction. NGOs also known as voluntary sector have become an irresistible global force today and it is growing in relation to its presence in developmental activities. Basically, an NGO or voluntary organisations are non-profit making agencies that are constituted with a vision by a group of likeminded people, committed for the uplift of the poor, marginalized, unprivileged, underprivileged, impoverished, downtrodden and the needy and they are closer and accessible to the target groups, flexible in administration, quicker in decision making, timely in action and facilitating the people towards self reliance ensuring their fullest participation in the whole process of development. SHGs are started by NGOs that generally have broad anti-poverty agendas. SHGs are seen as instruments for goals including empowering women, developing leadership abilities among poor people, increasing school enrollments and improving nutrition and the use of birth control. SHGs are small group of individual members who voluntarily come together and form an association for achieving a common objective. In most cases, SHGs are constituted by persons known to one another and coming from the same village community or neighbourhood. SHGs are small in size with membership ranging from 10 to 20 and are homogeneous. A strengthening development among women the nation over has been currently turned by quick advancement in SHG arrangement. The arrangement of SHGs isn’t conclusively a miniaturized scale credit venture however a strengthening procedure. The strengthening of women through SHGs would give advantage not exclusively to the individual women yet in addition for the family and network all in all through aggregate activity for improvement. Women constitute half of any country’s human endowment. All round development and harmonious growth of a nation would be possible only when women are considered as equal partners in progress with men.

Introduction

A large number of institutions in the field of agriculture and allied sectors are contributing to research in development of high yielding varieties of crops, technological innovations and other initiatives to boost production and human resource development. The technology available has to be permeated depending upon the necessities of the region – its soil, climate, culture and needs and means of the farmers, available human resources, feasibility and viability of different parts of the country. It has been observed that there are variations in knowledge and technological percolation. The National Commission of Farmers (founded in 2004) raised the issue of knowledge deficit, which directly impinges on agriculture productivity. There are significant gaps in backward and forward linkages between the agricultural laboratories and the farmers, in so far as transfer of technology is concerned. The 10th and 11th plans have emphasized the need for effective extension services. The 11th Plan Approach Paper also states that “in the longer run, growth in agriculture productivity can be sustained only through a continuous technological progress”. The 11th Plan shows a concern towards the problem of transfer of technology and knowledge at grass-root level and puts forth challenges before the extension agencies. The 11th Plan points out that extension services are to be treated as a service delivery mechanism. The 12th Plan also emphasizes the need for mechanisation of agriculture with robust extension services.

Introduction

Agriculture has been undoubtedly the major source of livelihood in the developing nations like India. Livelihood is defined as adequate stock and f low of food and cash with an individual or a family to meet its basic needs (Girish et al., 2020). It encompasses the capabilities, assets (stores, resources, claims and access) and activities required for a means of living (Chambers and Conway, 1992). Livelihoods are considered secure when households have secure ownership or access to resources and income earning activities, including reserves and assets, to offset risks, ease shocks and meet contingencies (Chambers, 1989). The livelihood security is a complex concept that is location specific, subjective and dynamic (Hogger and Ruedi, 2004). It comprises of many indicators like food, educational, financial, health, culturaland social securities. Constraints like practices of traditional methods of cultivation, lack of access to modern technology, low productivity of crops, in-adequate capital formation and low investment, widespread illiteracy among farmers, etc., have become a major threat to the educational, food, economic and social securities, thus ultimately affecting the livelihood status of farming households in the country (Hatai and Sen, 2008). Since, a large fraction of farmers in the country lives in the rural areas, they have no proper knowledge regarding the usage of modern farming techniques. Often their low level of education and lack of communication has resulted into a lack of general awareness regarding the modern research and inventions on agriculture (Makal et al., 2017). In such scenario, farmers’ trainings play important role which helps the farmers in understanding the application of latest agricultural technologies in daily farming activities. It improves the knowledge of the trainees about the improved farm practices (Srinivas and Sailaja, 2013). The major players providing these extension services in public sector are Krishi Vigyan Kendras (KVKs), State Agricultural Universities (SAUs) and ICT-led extension interventions by Department of Agriculture, Cooperation and Farmers Welfare (DAC&FW), Government of India. Many state universities through their KVKs, have been imparting need-based trainings to the farmers in their routine activities as well as sponsored by various departments/agencies. In last few decades, KVKs have increasingly become associated with grass root development (Saha, 2013).

Introduction

Horticulture crops cover large varieties of fruits, vegetables, flowers and plantation and spices crops. Among these, vegetable cultivation is the major attraction to farmers as it is comparatively more remunerative the field crops. Vegetables not only provide maximum output but also give more income per unit area of land. Vegetable cultivation among small land holders has always been source of supplementary source of income and provides gainful employment through intensive cultivation and thus vegetable growers are normally more prosperous than those who grow cereals, because of higher returns.

Brinjal (Solanummelongena L) is a commercial vegetable crop belongs to the family of solanaceae. It is a nannual crop. It is mainly used for culinary purpose. Brinjal has been considered as one of the important vegetable crop. The brinjal growers can increase production of brinjal through adoption of new varieties and with improved technologies. A first-hand knowledge regarding existing status of adoption of improved technology helpful to the extension workers for concentrating their efforts to create favorable condition for better adoption of the innovations of brinjal cultivation. Arvind kumar singh (2017) revealed that more than half of the respondents belong to middle age group, educated up to higher secondary, had medium level ofland holding, medium level of annual income, sources of information and low level of material possession. Further headded, that he possessed low level of farm power.

Introduction

The most significant commercially grown fruit crop in the country is the mango (Mangifera indica L.), which belongs to the Anacardiaceae family. It’s known as the “king of fruits.” Mango cultivars can be found in the greatest variety in India. Mango farming is thought to have begun in South East Asia. Since about 6,000 years ago, mango has been grown in south Asia.Mango is native to South Asia, from where it has been distributed worldwide to become one of the most cultivated fruits in the tropics.

The major mango-growing states are Andhra Pradesh, Uttar Pradesh, Karnataka, Bihar, Gujarat and Tamil Nadu. Andhra Pradesh rank 1st in area, whereas Uttar Pradesh rank 1st in production and productivity. Andhra Pradesh, Uttar Pradesh, Bihar, Karnataka, Maharashtra, West Bengal and Gujarat together contribute for about 82% of the total production in India.

India to become the second largest fruit producer in the world, after china, with a production of 102481 thousands metric tonnes of fruits from an area of 6930 thousands hectares in 2020-21.The fruit is grown in India, the total area under mango cultivation was 2317 thousand hectare with an annual production of 20386 thousand metric tonnes.(NHB 2020-21).

Mango is well adopted in tropical and sub-tropical climates but it can be grown up to 1,100 meters above sea-level. There should not be humidity, rain or frost during flowering. The temperature between 24 and 270C is ideal for its cultivation. Higher temperature during fruit development and maturity gives better quality fruits. The areas expressed frequent showers and high humidity is prone to many pests and diseases. Thus it can grow best in regions with a rainfall between 25cm and 250 cm. Regions having bright sunny days and moderate humidity during flowering is ideal for mango growing.The farmers 92.20 per cent had problems of pests in their mango trees. The most common pests were the mango seed weevil 48.10 per cent, fruit fly 36.40 per cent, trips 1.30 per cent, aphids 1.30 per cent and the most common diseases were the powdery mildew 46.80 per cent, anthracnose 18.20 per cent and scab 2.60 per cent (Gitonga,2010).

Introduction

Among the rice growing countries, India has the largest area (448 lakh ha) followed by china and Bangladesh. Total food grain production in India is estimated at 2813l.7 lakh tonnes. Rice production is estimated at 1156 lakh tonnes (Anonymous, 2010). In India, rice is grown in the state of Kerala, Andhra Pradesh, Orissa, Bihar, Uttar Pradesh, Madhya Pradesh, Punjab and West Bengal lead in the area, while West Bengal harvest highest rice production. The average yield per hectare is highest i.e., 5970 kg/ha in the Punjab during 2018-19.

Rice is grown throughout the state except Kachchh and Saurashtra. Some part of the state is growing rice as a drilled, while transplanted rice is taken in south and middle Gujarat. In Gujarat rice occupies about 10.61 per cent of the gross cropped area of the state and accounts for around 25.5 per cent of the food grain production. It is grown on an average about 7.5 to 8.5 lakh hector of land comprising nearly 70 to 80 per cent low land (transplanted) and 15 to 20 per cent of upland (drilled) rice (Anonymous, 2020). During the 2018-19, the yield of kharif rice in Gujarat was around 2247.75 kg/ha. Yield of summer rice was 3130.96 kg/ha and total production of rice in the year of 2018-19 was 1911 tonnes (Anonymous, 2020). During the 2019-20, the yield of kharif rice was 2117.81 kg/ha and yield of summer paddy was 2996.36 kg/ha and total production of rice in the year of 2019-20 was 1929.98 tonnes (Anonymous, 2020).

In Gujarat some districts like Chhotaudepur, Tapi, Navsari have highest rice productivity which covered 1.279 lakh hector area with production and productivity respectively of 3.338 lakh tonne and 2582 kg/ha and some district like Mehsana, Narmada, Panchmahal, North Gujarat etc. have medium to low productivity of paddy. But in some part of North Gujarat like Gandhinagar and Sabarkantha have low productivity of rice.

Introduction

Agriculture is the back bone of Indian economy. About 18.8 percent (2021-22) of the national income originates from the agriculture sector. Approximately 60 percent of India’s population is directly or indirectly dependent on agriculture for livelihood. A large number of important industries like jute, textiles, edible oils, tobacco, sugar etc. receive the raw materials produced by agriculture sector. India occupies second position in the production of fruits and vegetables in the world after China (National Horticulture Database 2019 2020) It is grown almost in all the states of the country.

Cauliflower (Brassica oleracea L. var. botrytis) like all other Brassicaceae family crops has descended through introgression, mutation, human selection and adaptation from a single wild predecessor Brassica oleracea L. var. sylvestris which is still found growing in western and southern Europe and North Africa. The name cauliflower consists of two Latin words namely ‘caulis’ means cabbage and ‘floris’ means flower. It is grown throughout the country for its highly suppressed ‘prefloral fleshy apical meristem’ called “Curd” which is used as vegetable, soup and for pickling (Choudhury, 2006). Cauliflower is the pride of cole crops because of its good taste, flavour and nutritional value. It contains good amount of vitamins like vitamin A, vitamin C and fair amount of proteins and fibers. The cauliflower is also a good source of minerals like Ca, Mg, P, Fe, Na, and S.

Introduction

Women make essential contributions to the agricultural and rural economies in all developing countries. Their roles vary considerably between and within regions and are changing rapidly in many parts of the world, where economic and social forces are transforming the agricultural sector. Agriculture needs manpower, if the manpower split into gender wise, amazing fact is that the women contribution is greater or equal to men (Chauhan, 2011).That is reason that right from the day the country become independent, it has been trying hard for rural development.

According to the Penguin Atlas of Women in the World, women make up 40 per cent of the agricultural labour force in most parts of the world, while in developing countries they make up 67 per cent of the agricultural workforce (Singh, 2008). Therefore, strengthening and expanding women’s roles and opportunities in agriculture promotes sustainable economic development. Gender inequalities are a measurable and significant limit on agricultural productivity and efficiency, undermining rural economic development. India has paved the way to development by increasing its trade and growth in Agriculture sector. To review the agriculture scenario of our country, it depicted that there is need to pay sincere attention towards the human resource development (men and women together) because modernizing agriculture needs skill and efficient work forced. Farm business has become a family enterprise in India, where both men and women take part evenly. It is well known fact that the success of rural development process largely depends on the participation of people at large, irrespective of sex. The problems of involving women’s participation in the development process are now catching the attention of planners and policy maker because of increasing imbalances generated out of development process. Now days, rural women play a vital role in domestic and socio-economic life of the society and therefore, national development is not possible without developing this important and substantial section of our society (Okafor, 2008).

Introduction

A woman is the nucleus of a family, particularly in rural India. For the rural women, the day starts early in the morning with the responsibilities of fetching water, fodder and fuel, cooking food and looking after the livestock and children. Promila et al. (2003) revealed that farm women spent 15:64 hrs/day and 16:58 hrs/ day for productive work during the slack and peak period, respectively. The time saved by farm women from farm activities during the slack period was diverted towards care of children, family members and household works. Still, there is no recognition for their hard work; just because their work is not evaluated in terms of money.

The contribution of women in agriculture is estimated to be about 50 to 60 per cent. In many places, the entire livestock management is looked after by women only. They help in farm operation, take their animals on distant lands for grazing, washing, milking, fodder, breeding management, sale of milk, if necessary by taking it to the market and so on in addition, perform the functions related to house management. Even though, women are playing a very important role in innovation decision process, they are not allowed to try and see whether they are capable of managing farm and family affairs independently or with the help of family members.Adequate attention is also not being paid for the social and economic development of farm women and to encourage them to avail training facilities on farm and dairy management practices for supplementing their family income through development of occupational skills and proficiency.

Introduction

India pre-dominantly an agriculture-based country, requires constant endeavors to seewhether rural and agricultural credit facilities are enhanced with time. As a part of theseefforts, agricultural credit cards and cash credit facility were introduced in lending system. The Kisan Credit Card is a credit card programme launched by Indian banks in August 1998. The National Bank for Agriculture and Rural Development (NABARD) developed this model scheme to supply term loans for agricultural needs based on the suggestions of the R.V. Gupta Committee. Its goal is to provide financial assistance to farmers in order to address the agricultural sector’s comprehensive credit needs. All commercial banks, Regional Rural Banks, and state cooperative banks are among the participants. Short-term credit limitations for crops are in place, as are term loans. Personal accident insurance covers KCC credit card members up to Rs. 50,000 for death and permanent disability and up to Rs. 25,000 for other risks. The premium is split evenly between the bank and the borrower. The validity period is five years, with the option of an additional three year extension, Kisan Credit Card provides cash and term credit to farmers for a variety of linkedoperations such as pump sets, land expansion, planting, and drip irrigation. Soil Wellbeing Card programming has been institutionalized and online programming built for eight statesunderthee-Government Program to provide coordinated supplement administration suggestions based on soil test edit reaction approach. Ranchers receive data through avariety of channels, including Common Service Centres, Internet Kiosks, and SMS, as partofthe Nationale-Governance Plan for Agriculture. Farmers are spared from the exorbitant interest rates charged by traditional banks under the KCC plan. The interest rate on KCC loans ranges from 2% to 4%. This low interest rate aids farmers in repaying loans more quickly, taking into account the crop’s harvesting season and the date the loan was provide.

Introduction

Owing to several biotic and abiotic stresses, the problem of agrarian distress in India has accentuated in recent years. The Indian government set a target of doubling farmers’ income by 2022–2023 in its annual budget for 2016–17 and started a number of initiatives to achieve this goal, including better irrigation efficiency, crop insurance, market infrastructure improvements, organic farming promotion, soil health restoration, and others. The pathway for doubling of farmers’ income encompasses several dimensions, from production to post-harvest management. These include: bridging yield gap, crop diversification, improvements in total factor productivity and proper management of irrigation (GoI, 2007, Evenson et al. 1999) alongwith the provisions of market and institutional support for efficient post-harvest management. With respect to Himachal Pradesh, in order to improve the productivity levels of existing crop enterprises and promote the diversification of cropping systems, various steps have been taken by the line departments which include the creation of water harvesting and irrigation infra-structure, skill up gradation of farmers, subsidy on critical inputs like seed, fertilizers, plant protection materials, market access through information technology and village road network, crop insurance schemes, etc. The impact of these interventions is reflected through the continuous increase in the productivity levels and inclination of farming community towards the adoption of new crop enterprises vegetables and fruit crops.

Introduction

Agriculture is the main occupation in India which engages nearly 119 million of farmers and 144 million landless labourers (Census 2011). According to the Agriculture Census (2012), about 80 per cent of Indian farmers fall under the small and marginal category. The average size of land holding in India is 1.15 ha which will tend to decrease in coming years due to land fragmentation, industrialization and urbanization which are forcing farmers to quit farming and look for more viable livelihood options (Bhattacharyya et al. 2018). Specially small and marginal farmers are exposed to high risk of farming due to climate vagaries, uncertainties in production and market demand, lack of access to support services and markets, limited scope of farm mechanization and the consistent failure of half-hearted government efforts which have been unable to make farming an exciting profession to the old as well as new generations. In Indian context, agriculture has never been a profession for the literate section of the society due to lower production and profitability. NSSO report (2012-13), in alignment with the current scenario, says 40 per cent farmers want to quit agriculture if they get alternatives.

Owing to several biotic and abiotic stresses, the problem of agrarian distress in India has accentuated in recent years. Government of India has taken initiative in its annual budget of 2016-17 and initiate a number of steps to alleviate agrarian distress and improve the situation before the fight for food security faces disastrous challenge from mass migration of Indian farmers to other occupations. The steps taken by the Government of India are improving irrigation efficiency, providing crop insurance, improving market infrastructure and its efficiency, promoting organic farming, restoring soil health and so on.

Introduction

The agricultural sector in India has focussed more on agricultural output and food security. From the year 1965 to 2016, India’s food production was multiplied by 3.7 times while the population was multiplied by 2.55 times. More than one f ifth of rural households with self-employment in agriculture as their principal occupation were having income less than the poverty line (NSSO, 2011-12). In the early 1980s, the farm income per cultivator was 34 per cent of the income of non agricultural workers. In the year 1993-1994, the relative income of farmers was one-fourth of the income of the non-agricultural workers. According to SAS 2012 2013, the average annual income of farm households from the farm as well as non farm sources was Rs. 77,112 which is 60 per cent from farm activities and 40 per cent from non-farm activities. In April 2016, the Government constituted an Inter Ministerial Committee to examine issues relating to DFI and recommend strategies to achieve the same. The target was to change the farm to non-farm income ratio from the existing 60:40 in 2015-16 to 70:30 by 2022-23. A seven-point strategy was proposed and several initiatives were taken to increase the income of farmers. The last available estimates on the income of agricultural households are based on the Situation Assessment Survey of Agricultural Households conducted by the National Sample Survey Office (NSSO) during 77th round (January – December 2019). As per the survey results, the average monthly income per agricultural household from all sources was estimated to be. Rs. 10218. Thus, the present study was taken to know the awareness, knowledge and perception of farmers towards doubling farmers income. Understanding the farmers awareness, knowledge and perception would help to develop specific strategies for increasing the farmers income.

Introduction

Pulses are the most important part of our diet, richest and cheap source of proteins, vitamins, minerals and amino acids besides high nutritional value. Hence, the economic value of these crops is very high. Pulses can be produced with a minimum use of resources and are mostly cultivated under rainfed conditions and do not require intensive irrigation (Sood et al. 2018). Keeping in view large benefits of pulses for human health; the United Nations has proclaimed 2016 as the International Year of Pulses. Thus, due attention is required to enhance the production of pulses not only to meet the dietary requirement of proteins but also to raise the awareness about pulses for achieving nutritional, food security and environmental sustainability. The Government of India through Indian Council of Agricultural Research (ICAR) has established a wide network of Krishi Vigyan Kendras (KVKs) in all the rural districts of the country. These KVKs under the aegis of the National Agricultural Research and Education System are the real carriers of frontline technologies and impart knowledge and critical input support for the famers. The frontline demonstration programme (FLDs) in pulses is a unique programme by Ministry of Agriculture, Govt. of India, conducted under close supervision of farm scientists. Main objective of FLDs in pulses is to demonstrate and popularize the improved agro-technology on farmers fields under varied farming situations for effective transfer of generated technology and f ill the gap between improved technology and adopted/indigenous technology to enhance pulse productivity and farms gains for sustaining the production systems especially under rainfed farming (Choudhary et al. 2009). The field demonstrations conducted under the close supervision of scientists of the National Agriculture Research System is called front-line demonstrations. The main objective of Frontline Demonstrations is to demonstrate newly released crop production and protection technologies and its management practices in the farmers’ field under different agro-climatic regions and farming situations.

Introduction

Onion (Allium cepa L.) is one of the most important commercial vegetable crop cultivated extensively in India and it belongs to the family Alliaceae. It is used both in green and mature stage for salad and spice in a variety of flavoured dishes and soups Onion is liked for its flavour and pungency which is due to the presence of a volatile oil ‘allyl propyl disulphide’- an organic compound rich in sulphur. Onion bulb is a rich source of minerals like phosphorus, calcium and carbohydrates. It also contains protein and vitamin C. It is being used in several ways as a fresh, frozen, dehydrated bulbs and green bunching types. Dehydrated onion is in great demand which reduces transport cost and storage losses. Onion has got good medicinal value. It contains several anti-cancer agents which have shown to prevent cancer in animals. The beneficial compound called ‘quercetin’ present in onion has shown to be powerful antioxidant. Extracts of onion are being used in the prevention of ‘atherosclerosis’ and ‘coronary heart disease’. onion extensive culinary, dietary, therapeutic, trading, income and employment generation value. Onion is commodity of mass consumption and is grown almost all over the country mainly by small and marginal farmers (Shukla and Singh-2018).

A global review of onion area and production revealed that onion is grown in the world in an area of 43.64 lakh hectares with a total production of 873.44 lakh tons and productivity of 21.79 tons/ha. China and India are leading onion growing countries with a total production of 247.00 and 159.40 lakh tons, respectively during the year 2017. India produces around 174 lakh tons of onion annually ranking second in the world. Currently in India the production of onion 31129 million tons with the cultivated of 1914 lakh hectares in 2021-22 observed respectively. Among the different onion growing states Maharashtra tops in area and production. (https://www.districts of india.com).

Introduction

Rice (Oryza sativa L.) is one of the most important cereal grains in the world today and serves as a staple food source for more than half of the world’s particularly in India, China and a number of other countries in Africa and Asia. It occupies an area of about 161 million hectares at global level wi`th production of 503.8 million tones milled basis. More than 90% of the rice is produced and consumed in Asian countries. (FAO United Nation 2017).

Rice provides 21 percent of global human per capita energy and 15% of per capita protein. Although rice protein ranks high in nutritional quality among cereals, protein content is modest. Rice also provides minerals, vitamins, and fiber, although all constituents except carbohydrates are reduced by milling.

Rice commodity recognized as a supreme commodity to mankind, because rice is truly life, culture and tradition. It has its own history and religious importance in human life. India is one of the world’s largest producers of white rice. For India, like many other developing countries, the issue of feeding ever increasing population is of prime importance, this problem can be solved by maximizing agricultural production through use of high yielding varieties and adopting improved package of practices of rice crop. India is facing the challenges of food and fodder production to meet the demand of rising human and cattle population. One of the major causes of this problem is low level of adoption of improved agricultural practices by the farmers. Population www.thecropsite.com

There are three seasons for growing rice in India viz. autumn, winter and summer. The main rice growing season in the country is kharif. It is known as winter rice as per the harvesting time. The sowing time of kharif rice is June – July and it is harvested in September - October. India is an important centre of rice cultivation. The rice is cultivated on the largest areas in India. Historians believe that while the indica variety of rice was first domesticated in the area covering the foothills of the Eastern Himalayas (i.e. North-Eastern India), stretching through Burma, Thailand, Laos, Vietnam and Southern China, while the japonica variety was domesticated from wild rice in Southern China which was introduced to India. Perennial wild rice still grows in Assam and Nepal.

Fish are Sentient Animals

There is strong increasing evidence that finfish, like other vertebrates are sentient animals. This means that fish are self-aware, they can feel pain and distress, they have long term and short-term memory and to some extent, they can experience emotions.

Fish are intelligent, sensitive creatures and like many other animals, they explore, travel, socialise, hunt and play. Some species care for their young and use tools as humans do. Fish are sentient animals capable of suffering and feeling pain. Most fish have highly developed senses with excellent taste, smell, hearing and colour vision. Until fairly recently, many people didn’t realise that fish were sentient or feel pain, and the mental abilities of fish were given limited attention by the scientific community. Now, recent discoveries open up a new world of understanding. Far more complex than we ever realised, f ish live rich social lives: communicating; hunting cooperatively; and, in some cases, developing cultural traits. Consensus arrived in the mid-2000s around science demonstrating high-level pain perception in fish, and examinations of fish behaviour are beginning to reveal a picture of fish as complex, social and emotional beings. Appreciation of these facts are not widespread in civil society, but identified in time for the principle of fish welfare to be recognised by legislation by the European Union.

Any technique that uses living organisms or substances from these organisms to make or modify a product or to improve plant or animal or to develop microorganism for specific uses, can be termed biotechnology. Biotechnology involves microbes or cells derived from plants and animals for efficient production of useful products for mankind. The use of microbes for production of alcohol and penicillin, to name a few, is well known. However, in the last 25 years, several novel organisms, yielding useful products have been created by genetic engineering. Today, there is a wide application of biotechnology in human medicine, agriculture and animal husbandry in the area of diagnosis prophylaxis and therapy.

Aquaculture industry has undergone a sea change world over in the last three decades. The important needs of the industry are development of fast growing disease resistant fish and shellfish varieties, development of cheap and effective vaccines, disease diagnostics, cell lines sand probiotics. Efforts on these lines have already begun since 3-4 decades and results are highly promising. Some of the biotechnologies employed in aquaculture health management (Plate 15) are fish cell lines, monoclonal antibodies and nucleic acids for diagnostics sub unit and nucleic acid vaccines for prophylaxis, transgenes for disease resistance, gene editing in fishes and their pathogens and probiotics for bioremedial measures.

A. Cell Lines for Health Management

Cell lines are transformed cells that are adapted well to grow and multiply outside the host in in vitro culture systems. Cells lines can be developed from fast growing tissues of young fish. There are two methods of developing cell lines (1) Explant method, (2) Enzymatic method. Cell lines are grown in sterile plastic or glass containers containing appropriate medium having nutrients salts, antibiotics, buffers, serum etc. Minimum essential medium (MEM) is the common medium employed for fish cell line. Cell lines are developed, characterised and deposited in national and international repositories, which can be procured for use in research and industry.

Introduction

Aquaculture is given major thrust in many countries including India not only as a source of good quality food of animal origin but also because of economic gains it brings to aqua-farmers, creation of employment and export trade. It has emerged as one of the fastest growing food producing sector sowing to intensification and diversification of culture systems. However, the success of the intensive aquaculture system depends on several factors including species cultured, type of feed, water quality, management practices and strategies adopted to prevent/ control disease problems. Further, the intensive aquaculture operation results in accumulation of wastes leading to water quality deterioration, oxygen depletion, build-up of toxic metabolites such as hydrogen sulphide, methane, ammonia and nitrites that cause stress to culture animals leading to susceptibility to diseases. Among the several challenges that seriously affect the growth and sustainability of aquaculture, the incidence of microbial diseases are considered as major threat. Thus, keeping cultured aquatic animals in good health becomes major requirement for the success of aquaculture.

Though application of chemicals and drugs are often considered necessary to maintain water quality and animal health, use of these, in the long run are known to cause harmful effects both to the animal and the environment. Further, accumulation of drugs and chemicals in tissues, development of resistance to drugs by pathogens, pathogen build up, destruction of useful microorganisms, deterioration of pond environment and toxicity issues have limited their use in aquaculture system. Under these circumstances several alternative strategies have been proposed as viable option for the success of aquaculture in the form of prophylactic measuressuch as use of probiotics, prebiotics, synbiotics, immunostimulants and bacterial vaccines. The term prophylaxis refers to all preventive steps taken including better aquaculture management practices that are adopted during a hatchery and farming operation to improve growth, minimize the load of pathogens and prevent occurrence of diseases.

Farming of fish and shellfish has gained significant grounds in several parts of the world. However, the major bottleneck to successful farming is disease in farmed aquatic animals. In recent years, lot of attention is being given to health management using various forms of immunoprophylactic measures such as vaccination and immunostimulation. To rationalise immunoprophylactic applications, it is vital to have insight into the specific and non-specific defense mechanisms of farmed aquatic animals. Through disease process studies it is very well known that a pathogen can cause disease only if it can overcome the non-specific and specific defence barriers of the host and successfully establish and proliferate. Non-specific and specific immune systems are the two main branches of the defense system. The immunity which an organism derives against a pathogen is the result of a delicate interaction and cooperation between the two systems.

Defense System of Fish

Surface Barriers as Local Immune System

The immune systems present in the surface structures of fish have their potential importance in local immune responses. Important surface barriers are skin, gills and gastrointestinal tract.

Introduction

Scope and importance

Aquaculture is breeding, rearing and husbandry of aquatic animals such as f ishes, crustaceans and molluscs for food, ornament and sports. It is a husbandry involving special animals, the cold blooded vertebrates and invertebrates, whose physiology, growth, metabolic activities and overall performance depend heavily on ambient aquatic temperature and environment. Therefore, husbandry and the health management of aquatic animals is quite different from that of the other land based warm blooded animals.

Aquaculture is one of the fastest growing food production sectors in the world including India. Three decades ago, few species were cultured in India mainly in inland waters and then aquaculture was contributing less than 10 % of total f ish production in the country. In the last 25 years, there is a rapid growth in aquaculture with annual rate of 7-8% with significant contribution (above 60%)to total fish production today. The number of species cultured today in India including ornamentals is close to 40. Besides, a good number of plant and animal fish food organisms are cultured to support the hatcheries and nurseries in aquaculture. The area under aquaculture is increasing steadily in inland and coastal brackish water while marine water is also being added up for aquaculture slowly and steadily. Very interestingly, non-traditional environment such as manmade saline soils are also explored successfully for aquaculture. Intensification of culture activity with high stocking density artificial feed and fertilizers is another dimension of aquaculture noteworthy. Large scale movement of aquatic animals and their raw or processed products across national and international borders is important from aquaculture production and health point of view. In general, aquaculture package of practices alter the very water in terms of its chemistry and microbial load and has significant negative impact on the health of fish in culture system.

Introduction

Nucleic acid based diagnosis is highly specific, sensitive and rapid method widely used in health management. The detection is mainly based on structure and sequence of bases of nucleic acid which are unique to an organism. In recent years, nucleic acid based diagnosis is becoming common and popular in fish and shell fish health management. Commonly employed nucleic acid based diagnosis are DNA hybridisation and Polymerase chain reaction (PCR).

Nucleic acids are made up of nitrogen bases. The primary sequence of bases in DNA or RNA have regions which are unique for a particular organism or gene. The sequence of DNA is composed of a series of four phosphorylated bases Adenine (A), thymidine (T), guanidine (G) and cytosine (C). Pairs of bases (A=T, or G=C) can form hydrogen bonds, which cause opposing strands of DNA to form a semi-stable bonding (hybridization or annealing) to one another. If the opposing strands of DNA match exactly, then the familiar double helix form of DNA results. In the following example, the opposing strands in (1) will form a double stranded, tightly bonded structure, and the DNA strands are described as complementary. In (2) the strands are non-complementary (opposing A=T and G=C base pairs do not line up) and the two strands will not bond.

DNA Hybridisation

The basic principle in DNA hybridization is that, if a strand of double helix of DNA is separated from its complement (by heat) it will bind (reanneal or hybridize) only to its complementary strand (or to a duplicate of its complementary strand). Two complementary RNA structure will hybridise in the same manner. Inaccurate and mismatched bindings will be unstable and fall apart.

The binding of one DNA strand to another is in some respect is analogous to binding of antigen and antibody. However, in the case of nucleic acid bindings, the precise molecular nature of binding (base pairing) is known and can be easily manipulated by choosing DNA strand of appropriate length and by incubating the strands in appropriate salt concentration, temperature. The stringency (faithfulness of the match) of hybridisation can be precisely defined and as a result coincidental cross reaction is virtually eliminated.

Introduction

Development and standardisation of diagnostics for aquaculture health management have undergone great improvements over the last three decades. Diagnosis in aquaculture health management involves several stages starting from farm-level observations to conventional laboratory identification and histopathology to sophisticated modern molecular tools. Accordingly, diagnosis can be broadly categorised under three levels depending on the level of scientific development.

1. level-I detection system, farm site observation and record-keeping;

2. level-II system, conventional laboratory identification employing microbiology, parasitology, and histopathology and

3. level-III system, advanced and specialized immunological and biomolecular techniques.

The interaction of an antibody with an antigen forms the basis of all immunochemical techniques. Antibody binds specifically to an antigen at epitope or antigenic determinant level and this property has been used for development of immunodiagnosis. Furthermore, as antibodies react with antigen at ambient conditions and do not require sophisticated equipment ideal field test kits can be developed. Biomolecular tools using nucleic acid probes although very sensitive are costly and time-consuming requiring sophisticated equipment and trained personnel. Against this background, there is always a need for developing simple, low-cost, rapid yet sensitive farm- level diagnostics. Such field-level tests facilitate screening a large number of samples by a large number of poor and medium-sized farmers who constitute a majority in developing countries. Large-scale screening besides empowering farmers is considered to be ideal for effective health management. Antibody probes provide an ideal format for developing simple field-level diagnostics as antigens ideally react with antibodies at ambient conditions without requiring sophisticated equipment. Available technology for raising large quantities of monoclonal antibodies (MAbs) against pathogens has increased the scope for developing field detection kits in human, animal and agricultural health management. Several farmer-friendly detection systems also referred to as point-of-care (POC) detection method based on antibodies have been innovated in the recent past. POCT is defined as ‘medical diagnostic testing at the time and place of patient care.

Disease diagnosis refers to the various procedures and techniques used to identify the nature of disease and to precisely pinpoint the primary and secondary pathogens involved. Disease diagnosis is an integral part of health management. Proper diagnosis helps to adopt accurate therapy and avoids indiscriminate use of chemotherapeutics. Information on case history and clinical signs should be carefully used while examining samples for diagnosis. By following rational and scientific sampling protocols, one can hope to arrive at accurate diagnosis. Sampling methodology is key to the success of proper disease diagnosis. Dead fish/shrimp should never be used for any diagnostic purpose. Autolytic post-mortem changes, saprophytic invaders, spoilage organisms, etc. mask the actual etiological agents and can lead to false diagnosis.

The three commonly used conventional diagnostic approaches are fresh examination using microscope, microbiological methods and histopathology. With little facilities and infrastructure, fresh microscopical diagnostic approach can be done at the farm/hatchery site itself. Gross examinations along with simple skin and gill preparations examined under microscope can many a times give very good information about the disease and the etiology. All fresh preparations should be made from live or moribund fish and the preparations should be wet and moist. The fish meant for examination should be kept wet and not allowed to dry. Many of the commonly occurring external disease problems can be easily diagnosed by this method.

Systemic bacterial diseases need to be positively diagnosed by adopting the microbiological approach. Samples for bacterial isolations should be taken from kidney, blood, body cavity, etc. from live or moribund fish following strict aseptic conditions. In shrimp, it is ideal to use hemolymph for bacterial isolations. Dominant bacterial isolates are identified following standard procedures and their antibacterial sensitivity tested before recommending any antibacterial therapy. This method requires a minimum of 4-5 days.

Introduction

Aquaculture and Zoonotic Diseases

Diseases that can transfer from wild or domestic animals to humans are referred to zoonosis (Fig 1). Zoonosis also includes biotoxin producing agents from fish to human beings which is well documented. There are a number of pathogens which spread from fish and shellfish to human and other domestic animals causing diseases.There are also many other infectious organisms of fish origin that have not been reported but have potential to infect and harm man. Zoonotic diseases originated from fish and shellfish have been documented in the past mostly from wild caught fish and shellfish. However, in the last 30 years as the capture fisheries is declining and aquaculture is growing steadily worldwide, zoonotic incidences from the latter are increasing.

Aquaculture growing at 6-7% annually world over including India is contributing to 50-60% of total fish production today. Additionally, aquarium industry is booming worldwide. Therefore,incidences and scale of zoonosis due to aquaculture are quite different from that from capture or wild fisheries. Disease outbreaks in cultured fish are often by pathogens indigenous to aquatic environment. In aquaculture, disease outbreaks are associated with quality and quantity of nutrients and high stocking density which increase pathogen load leading to easy transmission to human. Apparently healthy fishes may also harbor bacterial pathogens, especially in their kidneys and intestines. World Health Organisation (WHO) considers many diseases in aquatic animals as emerging type. Emerging diseases appear in a population for the first time or it may have been existed before, but is now increasing rapidly. Usually information on zoonotic potential of emerging diseases is limited which is essential to disseminate to human health managers. Potential biological contamination of aquaculture products can occur from bacteria, fungi, viruses, parasites and biotoxins. The location of the farm, the species being farmed, water temperature, husbandry systems, postharvest processing, and habits in food preparation and consumption are among the main factors influencing the zoonotic risk associated with aquatic animals and their products.

Epidemiology is the study of diseases in a population in its natural setting. In epidemiology, the population is the patient and hence epidemiology is population medicine. All epidemiological studies are field based. In aquatic epidemiology, aquaculture ponds serve as the laboratory. This can be a great advantage over laboratory studies, which cannot be easily extrapolated to field conditions.

Diseases are among the greatest deterrents to the sustained production in aquaculture. Diseases such as Epizootic Ulcerative Syndrome (EUS) in fishes and White Spot Syndrome (WSS) in shrimps have devastated aquaculture with bad impact on productivity and profitability. Various diagnostic and health management tools are being used to devise disease control strategies. It is becoming increasingly difficult to manage pathogens that have become endemic. Epidemiological approaches which have been very successful in human and veterinary medicine are now being increasingly recognised as important weapons that can be used in aquaculture to formulate disease control programmes.

Concept of Cause for a Disease

Cause from an epidemiological perspective is interpreted in quite a wide sense. This is somewhat different to the more traditional view of the role of cause being restricted to etiological agents. An epidemiological definition of a cause of a disease is “an event, condition or characteristic that plays an essential role in producing an occurrence of the disease”. Epidemiologists avoid defining the word cause for any disease outbreak, but prefer to use words such as determinants, exposures and risk factors. Alternatively, they will categorise causes as direct or indirect; necessary or sufficient; and single or multiple rather than defining primary etiology.

Introduction

Fish is the cleanest animal, gets bath 24 hrs a day provided water is clean without any toxic material. Perhaps this was the scenario world over 5 decades ago. However, in the last few decades due to industrialisation, modernisation of agriculture and animal husbandry, human health care industry and city developments all have contributed to toxic material to water endangering the fish and the consumer, leave alone clean environment. In recent years, even intensive aquaculture also add several chemicals, pesticides, antibiotics contributing to pollution. Natural water bodies such as rivers, lakes, reservoirs, scoastal/marine waters are ultimate receivers of pollutants which also serve as the source of water for aquaculture. Although pollution control by treatment measures and awareness have improved over the years still there is a long way to achieve the clean water. Effect of toxins in aquaculture has multiple angles, differing from that of toxins on land animal. Fish depends heavily on water for respiration, physiology, reproduction, food and feeding and its very sustenance- as a supporting medium.

Aquaculture is expanding world over growing at 6-8% gradually involving more areas and species contributing a major share to total fish production with gradual decline in capture fisheries from the natural water bodies. Invariably, most of the natural water bodies are polluted with one pollutant or the other in the country. In addition, several pollutants are added to the system by various aquacultural practices. Although large quantity of information is available on aquatic toxicology, toxicology in aquaculture from health point view of fish and the consumer is meager and not focused. Against this background, it is very important to study, understand various toxicants, their mode of action, accumulation and effect on fish and the consumer. Toxicology in aquaculture is incomplete unless it includes or addresses safety of the consumers. Further, poisoning of fish in farms and wild deliberately or otherwise needs forensic studies/approach to address the issues.

In the past several Non infectious diseases (NIDs) of fish and shellfish have been observed in natural waters. However, with expansion in fresh, brackish and marine aquaculture including aquarium keeping world wide there is a phenomenonal increase in incidences of NIDs and economic loss. Common Non infectious diseases (NIDs) in fish are 1.Gas bubble disease(GBD), 2. Body deformities(BD), 3.Nutritional diseases(ND) and 4. Disease due to harmful algal bloom(DHAB)

1. Gas Bubble Disease (GBD)

Gas bubble disease (GBD), is caused by supersaturation of gases in water. The gas involved is nitrogen in most cases and rarelycarbon dioxide and oxygen can cause GBD as these two gases are used by fish tissues and readily processed.Oxygen can cause gas-bubble disease at about 350 per cent air saturation, but nitrogen can cause the disease even below 118 per cent. GBD becomes prominent whenever there is a change in temperature, pressure and turbulance in aquatic environment, leading to disturbance in metabolism of f ishes. Bubbles of gas may form in the eyes, skin, gills, fins and within the internal organs where small bubbles may coalesce into larger bubbles.Fish absorb the excess gas, which forms bubbles in the small blood vessels, due to pressure changes inside their body. Gas bubble disease (GBD), is seen both in captive and wild fish. When bubbles affect internal organs, some fish may diesometimes without obvious clear clinical signswhile other fish may be chronically affected, and have a reduced appetite, activity and buoyancy. GBD can also cause secondary bacterial, viral or protozoan infections.All fish and shelfishspecies as well as amphibians and aquatic invertebrates are susceptible to GBD.Sensitivity of fish to GBD in terms of pathology and mortality varies with species and age.

Introduction

Oomycete and fungal diseases are considered as the second most economically impactful diseases in aquaculture, after bacterial diseases. However, among both the diseases, oomycetes are more common. The oomycetes are members of the Kingdom Chromista, which also includes diatoms, kelps, and brown algae. These group of pathogens form free-swimming zoospores and their cell walls are composed of cellulose and glycans rather than chitin. Fish-pathogenic oomycetes (Saprolegnia, Achlya, Aphanomyces and Branchiomyces), are commonly known as water moulds, are ubiquitously distributed in a variety of aquatic environments and ecological niches, and belong to the Order Saprolegniales. These pathogens have low host specificity and infect a diverse range of fishes. On the other hand, most of the fungi (Fusarium sp., Aspergillus sp., Candida sp., Paecilomyces sp., Penicillium sp. and Cladosporium sp.) responsible for infection in fish, form non-motile spores and their cell walls are made up of chitin. In addition, Ichthyophonus sp. an important pathogen which, superficially appears like fungus and produces spores and hyphae, belongs to a distinct clade of protistan parasites. Among the oomycete diseases, infection with A. invadans is considered as one of the most serious fish disease. Therefore, the disease is discussed elaborately.

Infection with Aphanomyces invadans: Infection with Aphanomyces invadans is popularly known as, Epizootic Ulcerative Syndrome (EUS). It has a complex infectious aetiology and is clinically characterised by the presence of invasive Aphanomyces infection and necrotising ulcerative lesions, typically leading to a granulomatous response (Plates 9 and 10). In an expert consultation held during the sidelines of the Fifth Symposium on Diseases in Asian Aquaculture (DAAV) at Gold Coast, Australia in November 2002, it was proposed to rename the disease as epizootic granulomatous aphanomycosis; however, the term EUS is still used popularly.

A. Viral Diseases of Shrimp

Shrimp farming which has grown to an industry in the last three decades is young compared to fish farming in the world. Yet the shrimp farming is facing serious problems with several types of viral diseases. Intensive culture in hatcheries and grow outs along with movement of stock across national and international borders of these animals with poor immune system has encouraged emergence of large number of viruses. During a short span of 30 years, 15 viruses of economic concern have been reported in shrimp culture of the Asia Pacific region. Shrimp viruses (Plate 8) can be broadly categorised into two groups:

1. Viruses infecting ectodermal (epidermis, hypodermal epithelium of fore and hindgut, nerve cord and nerve ganglia) and mesodermal (hematopoitic organs, antennal gland, gonads, lymphoid organ, connective tissue and striated muscle) origin tissues and replicating within the cytoplasm (YHV, TSV) and within the nucleus (IHHNV, WSSV), 2. viruses like MBV, HPV, BMNV, and BP infecting endodermal origin tissue and replicating within the nucleus of hepatopancreatic and mid gut epithelial cells. Lack of established shrimp cell line is the major drawback in study of shrimp viruses. Following are some of the common viruses causing diseases in shrimp hatcheries and grow out ponds in the Asia Pacific region.

Viruses are submicroscopic particles made up of nucleic acid covered with a protein coat. The nucleic acid is either RNA or DNA and not both. Protein coat is often covered with an envelope and depending on the presence or absence of an envelope, virus can be an enveloped or non enveloped virus. Size of the virus vary from 20-200 nm. Shape of the virus varies from a simple icosahedra to bullet shape to complex brick shape.

Virus are classified based on their nucleic acid (DNAor RNA) and protein profile. However, additional details on type of disease, host range, and geographic distribution of the virus are also used in classification. The classification of the virus is decided by the International committee on taxonomy of virus (ICTV).

Viruses are obligatory intracellular parasites requiring a living cell to replicate. As the virion is lacking its own metabolic machinery, it depends fully on the living host cell for machinery and raw materials for its metabolism and replication. This complex biology of the virus has made it difficult to clearly identify a viral process to design drugs for selective killing without damaging the host cell. Any attempt to kill the intracellular virus affects the host cell.

Replication of Virus in a Cell

There are two kinds of entry into and exit of virus from a cell: a) receptor mediated endocytosis (RME), b) direct entry by fusion. Non-enveloped virus enter the cell by RME (Fig. 1). RME of virus is similar to regular entry of other macromolecules into the cell. With this receptor mediated specified process, virus enter a cell in a vesicle which later fuses with lysosome. Inside the lysosome, viral particles undergo partial changes in protein coat due to digestion by lysosomal enzymes and move to cytoplasm. Finally, this uncoated nucleic acid replicates followed by synthesis of proteins and assembly of viral capsid proteins and genome to full blown virus particles. The site of assembly of the virus may be in the cytoplasm or nucleus of a cell which is referred to as inclusion body. Inclusion bodies which varies in size, shape, location and staining reaction can be used as a diagnostic feature for some viruses. Non- enveloped virus exit from a cell by damaging plasma membrane and hence clearly show cytopathic effect(CPE) in cell culture.

A. Bacterial Diseases in Shrimp

Bacterial diseases in hatcheries and farms are being increasingly recognised as major hurdles to successful shrimp farming (Plate 6). Among the several bacterial diseases, diseases due to Vibrio sp. (vibriosis) are common. As majority of the Vibrio are secondary opportunistic pathogens, problems related to vibriosis can be traced to stress, poor water quality and bad management. It is widely accepted that the ubiquitous opportunistic vibrios are always responsible for causing mortalities in shrimp which are already stressed by poor water quality, external fouling and primary pathogens like viruses.

Vibrios are gram negative, oxidase positive, motile rods. Most frequently reported species in hatchery situations include Vibrio harveyi, V. vulnificus, V. parahaemolyticus and V. alginolyticus. All species of cultured shrimp are susceptible to vibriosis under stressful condition.

The common clinical signs associated with vibriosis are: 1) high mortalities, particularly in PL and young juveniles shrimp, 2) Moribund shrimp appearing hypoxic and often coming to pond surface and edges, 3) Reddening of shrimp, 4) Shell and appendage necrosis with blackening, 5) Presence of luminiscence in affected shrimp in tanks and ponds.