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A family of about 175 genera and 2,500 species, Amaranthaceae are mostly distributed in tropical but also in temperate regions. About 18 genera and over 50 species have been reported from India. Some of the larger genera with the number of their approximately reported species and common names are Alternanthera (200, alligator weed), Gomphrena (100, globe amaranth), Iresine (80, gizzard plant), Amaranths (60, pigweed) and Celosia (60, cock’s comb).
Gomphrenas are 30-40 cm tall, bushy with erect stems, hairy, light green with oblong-ovate leaves tapering towards the base. Round button like or spherical flower head is a cylindrical spike 1-2 cm long and 1 cm in diameter and may be terminal and/or axillary, and are born on long, thin stems above the foliage. Each flower head has tiny, conspicuous, papery, colourful bracts which enclose the inconspicuous true flowers. Bracts ovate, three mm long, apex acuminate, bracteoles 4.5-5.5 mm long. Ovary is sub-globose with two stigmas. Seed is brown, lustrous, 1.5 mm long (Wagner et al., 1999). It has both C3 and C4 pathway.
Selected Indian Genera
Achyranthes, Alternanthera, Amaranths, Celosia, Digera, Gomphrena and Pupalia.

Food security of a country can be achieved through advanced agricultural research, followed by efficient and effective transfer of technologies. Unlike laboratory experiments, agronomic experiments are being conducted at open environment which leads to lot of variations in outcome/results due to influences of unpredictable weather condition and heterogenic soil characters even though proper adoption of local control. Results of an experiment can be expressed in different ways to make the clients to understand easily recordable output data viz., growth attributes, yield parameters and yield, laboratory analyses data like soil and crop nutrient status and quality parameters of the produce. Nutrient management studies should focus the efficiency of particular fertilizer nutrient over others, methods of fertilizer application, contribution of nutrients to crops from soil and fertilizer etc. Similarly, water management experiments would explain the water conveyance, application and storage efficiencies, suitability of method of irrigation, water saving techniques and water use efficiency of different crops as well as agronomic practices etc. In weed management experiments, the efficiency of particular herbicide in terms of controlling weeds, and increasing crop yields have to be expressed by various efficiency indices. Weed suppression ability of an intercrop can be explained by smothering efficiency.

Introduction

National Commodity & Derivatives Exchange Limited (NCDEX) is a professionally managed on-line multi commodity exchange offering commodity futures trading in a wide range of commodities viz, agricultural, base metals, precious metals, energy, ferrous metal, and others. The shareholders of NCDEX comprises of large national level institutions, large public sector bank and companies. Promoter shareholders of the Exchange include ICICI Bank Limited (ICICI), Life Insurance Corporation of India (LIC), National Bank for Agriculture and Rural Development (NABARD) and National Stock Exchange of India Limited (NSE). Other shareholders are: Canara Bank, Punjab National Bank (PNB), CRISIL Limited, Indian Farmers Fertiliser Cooperative Limited (IFFCO), Goldman Sachs, Intercontinental Exchange (ICE) and Shree Renuka Sugars Limited. NCDEX is the only commodity exchange in the country promoted by national level institutions. This unique parentage enables it to offer a bouquet of benefits, which are currently in short supply in the commodity markets. The institutional promoters and shareholders of NCDEX are prominent players in their respective fields and bring with them institutional building experience, trust, nationwide reach, technology and risk management skills. 

ILT is an acute viral disease of chickens, pheasants and peafowl characterized by marked dyspnea, coughing, gasping and expectoration of bloody exudate. The disease is worldwide.

Hosts

Chickens particularly susceptible; reported to occur in pheasants and peafowl.

Age Range

All ages susceptible but is most often found in adult (mature or nearly mature) commercial chicken layers.

Blood Sample collection

Blood samples (5 ml per goat) will be collected aseptically from the jugular vein in a vacutainer tube (B.D. Bioscience, Germany) containing EDTA as anticoagulant and transferred to the laboratory in an ice box.

Isolation of Genomic DNA

The Genomic DNA will be isolated from the blood samples by ‘Phenol: chloroform isolation’ method as described by Sambrook and Russel (2001). The steps for isolation of genomic DNA are as follows:

Molecular markers, including DNA based genetic markers have found their applications in almost all areas of biology, more so in evolutionary biology, genetics and breeding in order to identify a particular sequence of DNA in a pool of unknown DNA. For many applications, DNA markers are preferred over other molecular markers since DNA is stable within an organism over time and among stages of development whereas other molecules are dynamic. A molecular marker is a DNA sequence that is readily detected and whose inheritance can be easily monitored. The uses of molecular markers are based on the naturally occurring DNA polymorphism. Most commonly used molecular markers in biological science include:

1. Restriction Fragment Length Polymorphism (RFLP)

RFLP is the first molecular marker technique which was invented by Botstein et al. (1980). The technique is based on restriction fragment length polymorphism; a molecular marker based on the differential hybridization of cloned DNA to DNA fragments in a sample of restriction enzyme digested DNAs; the marker is specific to a single clone/restriction enzyme combination. It consists of the following steps:

Quantitative traits are governed by many genes or polygenes genes with small individual effects. They are often described by their gene action rather than by the number of genes by which they are governed. There are four types of gene action: additive, dominance, epistasis, and overdominance. Because gene effects do not always fall into clear-cut categories, and it should be pointed out that gene action is conceptually the same for major genes as well as minor genes, the essential difference being that the action of a minor gene is small and significantly influenced by the environment. A general way of distinguishing between these types of gene action based on interaction among alleles is as follows:

4.1 Introduction

The egg is made up of three primary components: the egg white, the egg yolk, and the egg shell. The calcite crystals of the shell are enmeshed in a protein and carbohydrate complex matrix. About 58% of the weight of an egg is made up of the viscous, colourless liquid termed egg white inside the shell. About 75% of the weight of an egg’s edible component is made up of water, although proteins and lipids make up the majority of its nutritional worth (Table 4.1). There are also trace quantities of minerals, often known as ash, and carbohydrates, which take the form of simple sugars including glucose, sucrose, fructose, lactose, maltose, and galactose.

? Cyanogenetic plants contain hydrocyanic acid or cyanogenic glycosides.

? Two forms of cyanide in plants- HCN( free form) and cyanogenic glycoside(bound form).HCN released from glycosides by physical disruption (mastication, trampling) , stress (drought, frost) or the action of an Hydrolyzing enzyme present in ruminal microflora beta glycosides and hydroxynitrile lyase.

? One of the most common plants poisoning among the grazing livestock.

? Cattle and buffaloes are most susceptible. Sheep and goats are less susceptible.

? Monogastric animals (pig, Horse) are relatively resistant due to destruction of the glycosides by the gastric activity.

? Poisoning occurs when HCN content in plants is high and ingestion of large amount of the plants in short period of time.

? Starved or underfed animal also ingest large amount of plants. Poisoning may also occur due to accidental ingestion of potassium cyanide.

Lighting is a fundamental aspect of filmmaking and photography, crucial for setting the mood and tone of a scene. Different lighting techniques can evoke various emotions and atmospheres, helping to tell the story visually. Here are some common lighting techniques and the moods they create:

Types of Lighting Techniques

1. Three-Point Lighting

• Key Light: The main source of light, providing the primary illumination.

• Fill Light: Reduces shadows created by the key light.

• Back Light: Adds depth by separating the subject from the background.

Term ‘Scientific research’ is too broad to be explained by using one or two definitions. One should go through the different expert views about scientific research in order to internalize the concept of scientific research. In essence a scientific research is characterized by its rigor, testability, replicability, precision, confidence, objectivity, generalizability and parsimony.

Research: Uma Sekaran (2006) defined research as an organized, systematic, data-based, critical, objective, scientific inquiry or investigation into a specific problem, undertaken with the purpose of finding answers or solutions to it.

Excretory system of an organism maintains its homeostasis i.e., a constant internal environment. Here in insects, the blood is haemolymph therefore it determines the excretion of an insect’s body. Excretion also eliminates the nitrogenous wastes and maintains the salt- water balance and also regulates the uniformily of haemolymph. The characteristic feature of insects is the presence of malpighian tubules to eliminate the metabolic wastes, whereas rectum walls carry out the function of reabsorption of salt and water. A minimum amount of water is excreted with nitrogen waste in order to conserve the water in body.

1.1. Introduction

The green revolution has led our nation from the state of deficiency to the state of self-sufficiency in food production. The grain production has increased substantially from around 50 million tonnes to about 220 million tonnes per annum. As per the latest estimates, annual fruit production is 44 million tonnes from an area of 3.72million hectares.

Depletion of nutrients, from the soil is steadily increasing due to the adoption of modem agricultural technology through intensive and extensive cultivation of high yielding varieties with chemically pure, micronutrients free high analysis fertilizers. So in recent years, the potentiality of many crops could not be exploited owing to the widespread nutrient deficiency observed in many parts of our country. With nutrient removals by crops exceeding the additions, there exists a perpetual negative balance. Consequently, nutrient deficiencies have multiplied in Indian soils and crops.

Soil is shaken with 0.15 % CaCl2 solution. Chloride ions displace adsorbed sulphate during extraction. Calcium ions suppress the extraction of soil organic matter and hence eliminate the contamination caused by extractable organic S.

Illuminative evaluation: The primary concern of illuminative or holistic evaluation is a description and interpretation rather than measurement and prediction of the totality of a phenomenon. It fits with the social–anthropological paradigm. The aim is to study a programme in all its aspects: how it operates, how it is influenced by various contexts, how it is applied, how those directly involved view its strengths and weaknesses, and what the experiences are of those who are affected by it.

While dealing with polygenic traits and the continuous variation in plant breeding programs, it is important to know as to what extent the phenotype is decided by the genotype and to what extent by the environment. It is well known fact that what we observe i.e. phenotype is the result of genotype, environment and interaction between the genotype and the environment. The total variance is phenotypic variance or the variance of phenotypic values and various components of variance are as shown in Table 6.1.

Terminal deoxynucleotidyl transferase (dUTP) nick end labeling (TUNEL) is a method for detecting DNA fragmentation by labeling the terminal end of nucleic acids. TUNEL is a common method for detecting DNA fragmentation that results from apoptotic signaling cascades. The assay relies on the presence of nicks in DNA which can be identified by terminal deoxynucleotidyl transferase or TdT, an enzyme that will catalyze the addition of dUTPs that are secondarily labeled with a marker. Here we perform the apoptotic assay test by TUNEL assay kit to identify the apoptotic property of any agent or chemical after treatment of primary cells culture at different concentration as per protocol provided with TUNEL Assay Kit and apoptotic cells are identified by using fluorescent microscope. Detailed procedure of TUNEL assay is as follows:

1. Preparation of primary cells culture as described in earlier methods.

2. Induction of apoptosis in primary cells culture is done by treatment of primary cells culture with different concentration of xenobiotics and incubation in CO2 incubator for 12 h as mentioned in micronuclei assay method.

3. The positive and negative control cells provided with the kit are already f ixed. However, negative control cells are prepared from control sample with absence of inducing agent.

4. Cell Fixation: Cell fixation is a required step in the TUNEL assay and it is done according to the procedure mentioned in the supplied kit by using paraformaldehyde solution. Suspend 1–2 × 106 cells (treated) in 0.5 ml of phosphate-buffered saline (PBS) and the cell suspension into 5 ml of 1% (w/v) paraformaldehyde in PBS and place on ice for 15 minutes.

5. Centrifuge the cells for 5 minutes at 300 × g and discard the supernatant.

INTRODUCTION

? Nitrate poisoning is generally caused when animals eat too much forage that is high in nitrates not changed to protein in the plant.

? Poisoning can also happen when animals eat too much urea or nitrogen fertilizer spilled in the field or left where the animals can find it.

? Nitrate fertilizer is palatable, especially to cattle.

? Nitrates are relatively non-toxic.

? Reduction of nitrates depends on the supply of fermentable energy as carbohydrates supply hydrogen ions for reduction of nitrates and production of nitrite in rumen.

1. Catla prefers zooplankton.

2. Silver carp prefers phytoplankton.

3. The blooming of diatoms causes golden brown colour in the aquaculture system.

4. Light or bright green colour of pond is due Chlorella (Green algae).

5. Dark green or blackish green colour is due to BG algae (Oscillatoria, Phormidium, Microcoleus).

6. Dark brown colour or sauce like colour is due to eutrophication.

7. Yellowish colour of pond water is due to growth in Chrysophyta.

8. Foggy white colour of pond is denoted by the mixture of zooplankton, clay particles and detrirus.

9. Japan is the biggest producer of marine finfish fry of 200 million fry per year (Pagrus major, Paralichthys olivaceus makes up 70% of total production).

10. Europe is a second biggest producer of marine fish fry for about 100 million fry per year.