eChapters

Introduction

Nitrogen-fixing cyanobacteria are the group of most primitive prokaryotes that have been observed in proterozoic era. Many Proterozoic oil deposits are attributed to the activity of Cyanobacteria, such as Gloeocapsomorpha. Small concentrically layered structures called pisolites are also the result of fossilized bacteria.

Access to information and improved communication is a crucial requirement for sustainable agricultural development. Modern communication technologies when applied to conditions in rural areas can help improve communication, increase participation, disseminate information and share knowledge and skills. It is being said that “Cyber Extension” would be the major form of technology dissemination in the near future. However it is observed that the rural population still have difficulty in accessing crucial information in order to make timely decisions. It is essential that information availability is demand driven rather than supply driven. The challenge is not only to improve the accessibility of communication technology to the rural population but also to improve its relevance to local development.

The advent of INTERNET on communication scenario offers enormous potential for two-way on-line communication between distant parties via the telecommunication and computer network spread over the entire globe. The world is rapidly shrinking to a ‘global village’ which some courageously even call a ‘global family’. The merger of communication (audio and video) and computer technology has suddenly made this combination so powerful that no sector of human activity can afford to ignore it. Right from high security defense systems, space research, engineering and educational applications the INTERNET has found users in petty shopkeepers and small service agencies. In countries like Singapore, you can book a taxi via e-mail and the concerned taxi driver is able to confirm your booking, while he is still driving.

ABSTRACT

Recent advances in Information and Communication Technologies (ICTs) have changed the way we live, learn and interact.  They have transformed the way knowledge is produced, processed, retrieved and transferred between different stakeholders. The innovative ICT applications are dominating and driving the development scholarship and thinking in agriculture sector. The increasing availability and use of the Information and Communication Technologies offers the Extension System and all its stakeholders/partners an undeniable opportunity to redefine the way it provides information and interacts with farmers. ICTs are not just a new tool for information dissemination; it is empowering and changing the very culture of society’s information-seeking behavior. And Extension must change, too.

e-Extension

E-extension can be defined simply as the use of electronic technologies (especially ICT) to enhance face-to-face and paper-based transactions. These technologies can be as simple as teleconferences or as complex as wikis and blogs.

Cyber extension (also known as e-extension) may be defined as extension over the cyber space, the imaginary space created by the interconnected telecommunication and computer networks.

Cyber extension

Cyber extension means extension over cyberspace. In the applied context of agriculture cyber extension means using of power of online networks, communications channels and interactive multimedia to improve information access for farmers, extension workers, research scientists and extension managers.

Advantages of cyber extension

1. Cyber extension will save time, money, and effort
2. Cyber extension reduces the dependence of the farmers on extension functionaries
3. Easily accessible in remote areas
4. Can provide an early warning system before the natural disaster
5. Cyber extension will be available continuously.

Intoduction Cybertaxonomy is an abbreviation for "cyber-enabled taxonomy." It shares the traditional goals of taxonomy: to explore, discover, characterize, name, and classify species; to study their phylogenetic relationships; and to map their ecological associations and geographic distributions. To improve the speed with which we can help in providing taxonomic products and information, there is undoubtedly a requirement to move taxonomic endeavour into the digital era. Currently, the rate at which we record and maintain morphological information that enables species discovery is the primary constraint on taxonomic productivity (Lasalle, et al., 2009). Cybertaxonomy is one attempt to accomplish this complex goal. Cybertaxonomy employs a collection of electronic tools to assist and equip the science of taxonomy in accelerating species invention and the implementation of taxonomic expertise in biodiversity investigation.Cybertaxomy can yield results more quickly and efficiently than ever before thanks to adoption of digital technologies and cybertaxonomy.

Cycads refer a group of plants belonging to the families Cycadaceae, Stangeriaceae and Zamiaceae as per recent classification. They belong to a specialized group of plants having ancient linkage. These are the most primitive of living gymnosperms and most ancient of all plants surviving today. Present day Cycads are mere remnants of numerous massive sorts that flourished during the end of Triassic era and belong to the Jurassic period.

Cycads include 11 genera and nearly 300 species distributed in the tropical, subtropical and warm temperate regions of the world. Substantial number of species is found in South Africa, Australia, South America, Central America, Caribbean Islands and Asia. There are natural hybrids also as reported from South Africa and Australia. Horticultural Importance

Cycads are curious and interesting both botanically and horticulturally all over the world. Their evergreen plam-like appearance with arching leaves from the crown looks graceful and ornamental. They are often mistaken with palm and tree ferns due to their structural similiraity. The distinctive forms emerged out of non-conventional stems and evergreen foliage is the main attraction. Primitive shapes of the Cycads are appealing to the landscapers. They are extensively used as a pot plant for indoor decoration and outdoor gardening. As Bonsai, Cycads exhibit a new style and formation. In horticultural trade, Cycads are in great demand and having high commercial value. Nowadays, a grown up specimen in large sized pot, may fetch several thousand rupees as price, which is very high in comparison to the other indoor plants. This is because of rarity, slow growing nature and graceful appearance of the cycads. Even the cut-leaves are in huge demand for various floral arrangements and decoration purpose. Therefore, Cycads are horticulturally very important and an essential component of present day landscaping. Selection of Plants

Cyclamen (Cyclamen persicum Mill.), belonging to family Primulaceae, is cultivated throughout the Temperate Zone. Many people have enjoyed the charming plants as potted and/or garden plants. The cyclamen is one of the most famous and important commercial ornamental plants in many countries. It is commonly grown for its flowers, both outdoors and indoors in pots. It is used on a terrace, in flower boxes, in hanging baskets or in beddings. Several species, particularly Cyclamen hederifolium, are hardy and can be grown outdoors in mild climates such as Northwest Europe and the Pacific Northwest of North America. The cyclamen commonly sold by florists is C. persicum, which is frost-tender. Selected cyclamen cultivars can have white, bright pink, red or purple flowers. The use as cut flower is not important part of cyclamen production (Grey-Wilson, 2002), but not rare in Europe, especially in Germany. Some cultivars for cut flower production were bred in Europe, but cut flower cyclamen production is not popular in USA and Japan. Its popularity as pot plant led to many studies on the group, including cytology (Bennett and Grimshaw, 1991), hybridization (Gielly et al., 2001 and Grey-Wilson, 2002) and phenology (Debussche et al., 2004).

Cyclamen (Cyclamen persicum Mill.), belonging to family Primulaceae, is cultivated throughout the Temperate Zone. Many people have enjoyed the charming plants as potted and/or garden plants. The cyclamen is one of the most famous and important commercial ornamental plants in many countries. It is commonly grown for its flowers, both outdoors and indoors in pots. It is used on a terrace, in flower boxes, in hanging baskets or in beddings. Several species, particularly Cyclamen hederifolium, are hardy and can be grown outdoors in mild climates such as Northwest Europe and the Pacific Northwest of North America. The cyclamen commonly sold by florists is C. persicum, which is frost-tender. Selected cyclamen cultivars can have white, bright pink, red or purple flowers. The use as cut flower is not important part of cyclamen production (Grey-Wilson, 2002), but not rare in Europe, especially in Germany. Some cultivars for cut flower production were bred in Europe, but cut flower cyclamen production is not popular in USA and Japan. Its popularity as pot plant led to many studies on the group, including cytology (Bennett and Grimshaw, 1991), hybridization (Gielly et al., 2001 and Grey-Wilson, 2002) and phenology (Debussche et al., 2004).

ORIGIN AND DISTRIBUTION

Cyclamen originated from extreme eastern Mediterranean region and naturally grows in countries such as Palestine, Syria, Israel, Greece, Turkey, Tunisia or even Mediterranean islands: Rhodes, Cyprus and Sicily (Grey-Wilson, 2002). Cyclamen was introduced in Europe at the end 16^th century, beginning of the 17^th century, passing first through France. Somewhat neglected in the following century, the cyclamen became fashionable again in France in the 1900s. But since 1860, the first variants of the botanical or “wild” cyclamen appeared in England, then in Germany, with much bigger flowers and with varying colour shades.

This chapter provides an introduction to cyclogenesis, nature of cyclone disaster and historic events. The mechanism that is in practice in cyclone mitigation, information dissemination and relief and rescue operations are detailed. As the state of Orissa is being battered more frequently by cyclones than any other state of India, special emphasis on cyclone disaster management system of Orissa is also discussed.

INTRODUCTION

Orissa state with immense natural resources is located between 17⁰27’N and 22⁰34’N latitudes and meridians of 81⁰27’E and 87⁰29’E longitudes. Orissa lies on the eastern coast of India with the waters of the Bay of Bengal swirling along its eastern and southeastern boundaries. It shares its boundaries with West Bengal and Jharkhand on the north, Chattisgarh on the west and Andhra Pradesh on the south. Out of the Indian mainland coastline of 7500 km, it constitutes a coastline of about 480 km. It extends for about 155,707 sq.km accounting about 4.74% of the total area of India. According to the 2001 census, it has 3.57 percent of the total population of India. Administratively, it has 30 districts, 3 Revenue Divisions, 58 Sub- Divisions, 51,061 villages and 127 Urban Centers.

Based on physiography, Orissa can be divided into three broad regions - the coastal plains, the middle mountainous region and the plateaus, and rolling up lands. The coastal plains extend from the River Subarnarekha in the north to the River Rushikulya in the south. The coastal zone covers nine districts namely Ganjam, Puri, Jagatsinghpur, Kendrapara, Jajpur, Khurda, Cuttack, Bhadrak and Balasore. It is narrow in the north, widest in the middle, narrowest in the Chilika coast and broad in the south. The coastal belt of Orissa lacks hills excepting few hillocks and mounds in the northern and southern parts. It has extensive low lands adjacent to the sea, sloping gently towards the shoreline of the Bay of Bengal. The coastline consists of the lakes, lagoons, marshes, bays and islands and natural harbors. The lakes in Orissa are natural and artificial. One of the blazing example of the natural lake is the Chilika lake.There is only one bay in Orissa which is the Hukitola bay. Eventually there are only two islands in Orissa - the Short’s Islands and the Wheeler Islands. Among the natural harbor, there is only one of its type in Orissa i.e. Paradeep which holds its recognition as the deepest natural port in the whole of India. The main drainage system of this area is represented by several rivers at their old stages flowing sluggishly towards the sea, which form topographic features like peneplains, natural levees, deltas, etc. (Fig.1). The major rivers flowing in the coastal belts are the Subarnarekha, the Budhabalanga, the Baitarani, the Brahmani, the Mahanadi and the Rushikulya. This region is the combination of several deltas of varied sizes and shapes formed by these rivers. The coastal belt has an area of 40,166 sq. km with a population of 68,79,379, which is 45 percent of the population of 9 coastal districts of Orissa.

These cyclones and anticyclones, the large-scale rotating weather systems, characterized by distinct patterns of atmospheric pressure and wind, exert a profound influence on ocean conditions that are directly relevant to fisheries. From the dramatic impacts of cyclones – with their powerful winds, heavy rainfall, and storm surges – to the more subtle but equally important effects of anticyclones on sea state and overall weather stability, understanding these systems is crucial for anyone involved in marine fisheries. For those of you aiming to work in aquaculture, fishing operations, coastal management, or marine conservation, a solid grasp of cyclones and anticyclones will equip you to anticipate and respond to significant environmental changes. This knowledge will be invaluable for ensuring the safety of fishing fleets, managing aquaculture facilities in coastal areas, and understanding the broader ecological impacts on fish habitats and populations.
In this chapter, we will explore
• The fundamental characteristics of cyclones and anticyclones: We will unravel their pressure systems, wind patterns, and overall structure.
• Their formation and life cycle: We’ll examine the atmospheric conditions that give rise to these powerful systems and how they evolve over time.
• The direct and indirect impacts on the marine environment and fisheries: This will include the effects of strong winds on sea state, the influence of rainfall and runoff on coastal salinity, and the devastating potential of storm surges on coastal ecosystems and infrastructure.1 We will also touch upon how anticyclones can lead to periods of calm weather that can also have implications for fishing activities.
Prepare to navigate the fascinating dynamics of these atmospheric giants and understand their significant role in shaping the marine world that underpins our fisheries. Let’s begin our exploration of the whirlwinds of change!

Cymbidium is a genus in the orchid family Orchidaceae. It was first described by Olof Swartz in 1799. The name is derived from the Greek word “kumbos”, meaning “hole”, “cavity”. It refers to the form of the base of the lip. The flowers are long-lasting, both on the plant and when cut large attractive and available in a wide range of colours. Smaller plants are now coming into vogue as decorative pot plants. These have smaller flowers but they are as desirable as the standard varieties and often more acceptable in modern homes. The popularity of miniature cymbidium is now spreading from Asia to the worldwide orchid community. These ancient flowers have been treasured in numerous societies for nearly two thousand years. Many orchid lovers are most charmed by its fragrance and form rather than its petite size.

Cymbidium is a genus in the orchid family Orchidaceae. It was first described by Olof Swartz in 1799. The name is derived from the Greek word “kumbos”, meaning “hole”, “cavity”. It refers to the form of the base of the lip. The flowers are long-lasting, both on the plant and when cut large attractive and available in a wide range of colours. Smaller plants are now coming into vogue as decorative pot plants. These have smaller flowers but they are as desirable as the standard varieties and often more acceptable in modern homes. The popularity of miniature cymbidium is now spreading from Asia to the worldwide orchid community. These ancient flowers have been treasured in numerous societies for nearly two thousand years. Many orchid lovers are most charmed by its fragrance and form rather than its petite size.

ORIGIN AND DISTRIBUTION

Cymbidiums figure in the earliest historical records of human association with orchids from China and Japan. Cymbidium native of the monsoonal forest foothills of the Himalayas with the warmer growing Asiatic species from the tropical lowland monsoonal forests. The species come from the Himalayan regions of India and Nepal running through China and Japan, Indo-China down to Malaysia to Australia. This genus is distributed in tropical and subtropical Asia such as northern India, China, Japan, Malaysia, the Philippines, and Borneo and northern Australia. The larger flowered species from which the large flowered hybrids are derived grow at high altitudes. They have been cultivated for thousands of years especially in China. Cymbidiums became popular in Europe during the Victorian era. One feature that makes the plant so popular is the fact that it can survive during cold temperatures as low as 7^oC. Originating in temperate and tropical parts of India, Nepal, China, Japan and down to Malaysia and Australia, cymbidiums are probably the most widely grown of all orchids because of their ease of cultivation and long-lasting flowers. From the 40-50 species, thousands of hybrids have been bred with flowers ranging in colour from white, yellow and orange to pink, red, brown and green, and varying in size from 5-10 cm across. There are several species existing in the Indian forest area. Several species are existing in the forest of Nagaland (Changkija et al., 1992), some of them are given in Table 1.

ABSTRACT

Cultivation of mat grass (Cyperus pangorei Rottb.) is valuable and expensive products can plays an important role in rural areas such as Sabong and Pingla in Paschim Medinipur of West Bengal in India. Mat grass plays an act to fill the empty employment field to the resource of poor farming community to support and secured their livelihoods and maintain the economical balance of their area. This grass usually cultivated by the poor and marginal farmers. The mat sticks are used to make various household utility items, such as floor and bed mats and other attractive goods like bags, purse, TV covers. Now a days the products prepared from this grass is exported to different countries. Mat sticks are attractive durable and is eco-friendly substances which easily gets decomposed by organisms, does not create any environmental hazards and gets more profit as compared to growing rice per annum. Even an old man or woman in rural areas can earn a net income of about Rs.120 to Rs150 per day. Major focus of the study is to establish the scientific principles behind processing, separation of clum strand, texture and strength properties of the mat fibers and increase mat production which leads to improve the economic condition of rural people.

Cystic ovarian disease (Cystic ovarian degenertaion or ovarian cysts) is a common ovarian dysfunction in cattle and causes huge economic loss to farmers due to its high incidence and negative impact on the reproductive performance of cow. Animals having cysts on ovary result in anoestrum as the most significant clinical sign. Althouugh the exact cause that leads to cystic ovarian disease is not yet fully clear but altered release of LH hormone is the most accepted hypothesis.

Cytauxzoonosis is an emerging tick-borne disease of domestic cats and wild felids caused by the protozoa of the genus Cytauxzoon. The common clinical findings in affected cats are similar to other hemoparasitic infections namely anorexia, depression, anemia, vomiting, icterus, and high fever. The first reporting of feline cytauxzoonosis with four fatal cases was done by Wagner in Missouri, USA in the year 1976.

Bobcats (Lynx rufus) are recognized as the natural wild reservoir of Cytauxzoon felis with prevalence reaching up to 100% in some enzootic areas (Zieman et al., 2017). This infection is highly fatal in domestic cats with 97% mortality without treatment (Meinkoth and Kocan, 2005). However, subclinical infection in some cats has also been evidenced i.e., they were infected with C? felis but did not display the acute clinical signs of the disease before diagnosis (Brown et al., 2010 and Rizzi et al., 2015).

Several other wild felids apart from bobcats and domestic cats have been found to be affected by fatal or nonfatal Cytauxzoon sp. infection. These include lions, jaguar, cougars, pumas, ocelots, Iberian lynx, meerkats, European wild cats and Asiatic wild cat, panthers, and tigers (Jakob and Wesemeier, 1996; Rotstein et al., 1999; Yabsley et al., 2006; Andre et al., 2009; Filoni et al., 2012 and Furtado et al., 2017).

Cytogenetics helps in solving intricate problems of evolution of economic plants and their relationship with relevant wild ancestors. Ornamental plants provide very interesting material for evolutionary studies. Such a study would reveal the nature of the evolutionary steps and clarify the changes that the breeding system and chromosomes underwent. The genetic and cytogenetic reasons for the diversity in ornamentals would also be unraveled. Such a study also helps to chalk out a meaningful breeding methodology for genetic improvement. A systematic programme of genetics and plant breeding of ornamental plants was started at CSIR-NBRI. The objectives of the programme were both basic or creative (experimental analysis of the causes of variation and evolution) and applied or productive (experimental synthesis of new and novel variation with better putative parents). Evaluation of colchiploids of ornamentals with different genetic systems was being carried out at CSIR-NBRI, with a view to explore the possibility of their introduction in floriculture trade. Superiority of polyploid cultivars, in general, has been the main factor, which has induced gardeners to raise artificial tetraploids. Most of the garden forms are of hybrid origin, raised over a number of years and named differently by different workers and nurserymen. More often than not, there is no authentic record of their ancestory and they show a great deal of morphological and cytological polymorphism (Khoshoo 1968, 1990, Datta and Banerji (1995). 

Introduction
• Cytogenetics is essentially a branch of genetics, but is also a part of cell biology/ cytology (a subdivision of human anatomy), that is concerned with how the chromosomes relate to cell behavior, particularly to their behaviour during mitosis and meiosis. Techniques used include karyotyping analysis of G-band chromosomes, as well as molecular cytogenetics such as fluorescent in situ hybridization (CGH). Cytogenetic approaches to studying chromosomes and their relationship to human disease have improved greatly over the past several decades.
As a mature enterprise, cytogenetics now inform genomics, disease and cancer genetics, chromosomes evolution and the relationship of nuclear structure to function. Cytogenetics involves testing samples of tissue, blood or bone marrow in a laboratory to look for changes in chromosomes including broken, missing, rearranged or extra chromosome. Cytogenetics may used to help diagnose a disease or condition, plan treatment or find out how well treatment is working.
Chromosomes
Chromosomes are the rod shaped; dark-stained bodies seen during metaphase stage of mitosis. The chromosomes are the nuclear components of special organization, individuality and function. They are capable of self-reproduction and play a vital role in heredity, mutation, variation and evolutionary development of the species. Chromosomes are thread-like structure located inside the nucleus. Each chromosome is made up of DNA tightly coiled many times around proteins called histones that support its structure. The number of chromosomes is constant for a particular species. Therefore, these are of great importance in the determination of the phylogeny and taxonomy of the species. The number of chromosomes varies from species to species but the number remains constant in a species. But sometime, due to certain accidents or irregularities at the time of cell division, crossing over or fertilization some alteration in morphology and number of chromosomes takes place. Humans have 23 pairs of chromosomes-22 pairs of numbered chromosomes, called autosomes, and one pair of sex chromosome, X and Y. Each parent contributes one chromosome to each pair so that offspring get half of their chromosomes from mother and half from their father.

Cytokines are the chemical messengers that are involved in interactions between the cells of innate and adaptive immune responses. They are secreted by leukocytes and many other cells in the body. They are low molecular weight proteins or glycoproteins which are produced in response to various kinds of stimuli. Cytokines have important role in immune and inflammatory responses.  Their functions are not limited to immune system only.They also stimulate the development of hematopoietic cells and also act as therapeutic agents.

A.    Answer the following.

Q.1.     What are the cytokines involved in innate immunity ?
Ans.     IL-1, IL-12, IL-6, IFN-a, IFN-b and TNF- a.

Q.2.     What are the cytokines involved in adaptive immunity?
Ans.    IL-2, IL-4, IL-5, IFN-g and transforming growth factor – b (TGF- b).

Q.3.     How does superantigen differ from conventional 
antigen?
Ans.     Superantigens bind to class II MHC molecules outside of the normal peptide binding cleft. Unlike normal antigens, they are not internalized and processed by antigen presenting cells but binds directly to class II molecules. A particular superantigen can activate 5-25% 

Cytokines are the short lived chemical messenger molecules, released by a group of cells which can modulate the functions of the same cell(by which they are produced) or other cells.

Cytological and wet mount techniques are essential diagnostic tools in Indian aquaculture, especially in regions with limited access to advanced laboratory infrastructure. These methods offer rapid, cost-effective, and straightforward approaches for identifying fish pathogens, making them highly suitable for both field and laboratory use.
Cytology involves the collection of cells from various fish tissues—such as gills, skin, fins, kidney, liver, and other internal organs—which are then smeared onto clean glass slides. The smears are air-dried, stained, and examined under a microscope to detect cellular abnormalities, inflammatory responses, or the presence of infectious agents.
• Common stains used in Indian labs include Giemsa, Leishman, and Wright’s, which effectively highlight the morphology of erythrocytes, leukocytes, and abnormal cells.
• Cytology can reveal conditions such as anemia, inflammation, and bacterial or fungal infections, particularly those caused by pathogens like Aeromonas or Edwardsiella.
• Swabs from liver or kidney are frequently used to observe inclusion bodies and aberrant cell structures that suggest systemic infections.
Wet mounts involve placing fresh samples—such as mucus, gill biopsies, skin scrapings, or fecal material—on a slide with a drop of saline or water, covered by a coverslip. These are examined under light microscopy to detect motile parasites and other abnormalities in real time.

Adrenal Tumours

• Adrenal cortical adenoma had normal secretory cells
• Most cells had naked nuclei in the abundant cytoplasm
• Basophilic cytoplasm with abundant clear lipid vacuoles
• Round uniform nuclei

Discrete Cell Tumours

(Round / discrete / distinctive / unique cell tumours)

General Features

• Exfoliate many cells
• Cells often quite small to medium sized
• Discrete cells
• Do not stick together
• If the smear is very cellular, cells are packed close together in sheets
• However, these cells are not adherent to one another
• There are sharp, crisp cytoplasmic margins between cells

Non-Neoplastic Conditions

Haematoma

Collection of blood in outside of the blood vessels It is more common in external ear

• Presence of erythrocytes, blood filled leococytes (non-degenerative neutrophils and macrophages) and platelets
  • Haemosiderin or haematoidin crystals will be seen

Epidermoid cyst/Epidermal cyst/Epidermal inclusion cysts/Follicular cyst

Cytologically cannot distinguish epidermoid cyst from dermoid cyst

• Constitutes ????V3 to V2 of non-neoplastic/non-inflammatory lesion
• Smear is thick
• Keratin bars, squames and other keratinocytes

Hepatocellular Carcinoma

• The presence of normal appearing hepatocytes of atypical hepatocytes with malignant characters
• Single to clusters of polyhedral or polygonal shaped or pleomorphic cells
• Large round, spherical or oval nuclei
• Higher nuclear cytoplasmic ratio
• Large nucleoli
• Dark blue cytoplasm

Points to Ponder

Collect cytological samples (FNAB) only from enlarged lymph nodes

Do not make attempt to collect samples from normal lymph nodes unless to study normal picture (Academic interest).

Care to be taken to hold the lymph node while collecting FNAB

Otherwise needle may go into perinodal fat yielding fat tissue so also in obesity

Submandibular salivary gland enlargement mimic submandibular lymph node enlargement

Salivary tissue will show epithelial cells, acinar formation and no lymphocytes

Cytological diagnosis of lymphadenopathies can be comfortably and confidently made.

Peripheral Lymph Nodes

Mesenchymal Tumours

General Features

• Do not exfoliate many cells - hypocellular; Low cellularity
• Embedded in extracellular matrix-osteoid, chondroid or collagen
• Single, discrete cells and a few are also seen in dense clumps i.e. Individual or unorganized cluster of spindle cells
• Cells-Elongated, fusiform/spindle shaped
• Cytoplasmic extensions/tails seen on both ends of nucleus; borders ill-defined
• Nucleus elongated/fusiform to oval
• As the malignancy potential increases cells become plumpy with a larger, rounder nucleus and shorter cytoplasmic tails
• Nuclei may be fusiform or oval.
• These tumors cannot be differentiated cytologically.
• Tentative diagnosis made.
• Well-differentiated malignant mesenchymal tumors may resemble actively proliferating granulation and requires histologic confirmation.

Mesothelioma

• Arising from the mesothelial lining of serous cavities
• It is very difficult to differentiate from carcinoma, reactive mesothelial hyperplasia
• Round to polygonal cells
• Arranged mostly in clusters
• Centrally located hyperchromatic nuclei

Testicular Tumours

Seminoma

• Cells were round exhibiting anisocytosis
• Round to oval nuclei
• Single distinct eosinophilic to basophilic nucleoli
• Cytoplasm scanty

Sertoli Cell Tumours

• Cellularity - High, anisocytosis
• Nucleus round to oval, megakaryosis, 1-3 basophilic nucleoli
• Chromatin coarse and granular
• Cytoplasm vacuolated

Canine Thyroid Tumours

• Occurs as carcinoma
• Blood contamination also seen
• Cluster of cells
• Free nuclei seen in the pale-blue cytoplasm
• Dark blue to black pigment present in the cytoplasm
• Amorphous pink materials represents colloid
• Colloid and pigmented granuls indicates the thyroid origin
• Round to oval nuclei

Location is important

Since, one should recognize whether cells belong to that area and / or not

Basic aim

• To establish an aetiological and/or morphological diagnosis in order to obtain a more accurate prognosis

Salient Points in Cytological Examination

• Cytology is a joint effort between the clinician and cytologist
• Adequate history, clinical signs and methods of collection should be intimated
• Interpretation suffers if done in a vacuum of information
• Cytology is an rapid, inexpensive technique in tumour diagnosis

Check the Following

Different types of Romanowsky type of stains are used for staining the cytological smears. It will give excellent cytoplasmic and nuclear details and it also stains bacteria. The following stains are commonly used. e.g., Wrights, Giemsa, Leishman, Wright-Giemsa, May-Grunwald-Giemsa, Leishman-Giemsa, Haematoxylin and Eosin, Diffquik stains, Trichrome stains, Papanicolaou or Sano modification.

Staining Methods

Leishman-Giemsa

• Cover the smear with LG stain for a minute
• Dilute with tap water/Distilled water/ phosphate buffered saline pH 6.87.2
• Allow to stain for about 20 min
• Wash in water

May-Grunwald-Giemsa Stain (MGG)
Fix slide in absolute methanol
Protocol
1) Fix the slides with May-Grunwald reagent (15 drops) for three minutes.
2) Without removing the reagent, the same amount of distilled water is poured on the slide and allowed to stand for three more minutes.
3) The dilute May-Grunwald reagent is poured off and the slide is immersed for 8-12 minutes in a watch glass containing Giemsa reagent (15 drops of concentrated reagent are used for each 10 ml. distilled water). The slides should be immersed upside down in the solution to prevent the precipitate formed in the staining mixture from adhering to the preparation.
4) The slides are washed with tap water and dried in air or with filter paper. The distilled water must be neutral or otherwise buffered. With bone marrow or lymph node aspirates a longer staining time is recommended on account of the greater cell concentration. We recommend controlling the staining time with the microscope.

Measuring cell size, diameter, length, distance between cells, area, etc, are important indicators of the identification of cell types and the physiological or pathological state of cells and tissues. ImageJ is a useful program that allows researchers and scientists to work with microscopy images for cytomorphological detailing.
The steps to measure cell size are:
A. Open Your Image in ImageJ: First, open the image containing the cells you want to measure.
B. Set the Scale (Optional): If your image has a known scale (e.g., if you used a microscope with a calibrated scale bar), you can set it inImageJ. This step ensures accurate measurements.
Go to Analyze > Set Scale. Enter the known distance (in pixels) and the corresponding physical distance (e.g., micrometers). Click OK.
C. Choose the Measurement Tool: ImageJ provides several measurement tools. The most common ones for cell size are: Area Measurement: Use the Polygon Selection Tool to outline the cell, and then go to Analyze > Measure.
D. Length Measurement: Use the Straight-Line Selection Tool to measure the length of cell projections (e.g., neurites or filopodia).
E. Record Measurements: After selecting the appropriate tool, click and drag to measure the cell area or length. Press’ t’ to record the measurement. Repeat for multiple cells if needed.
F. View Results: The measured values will appear in the Results window. You can access the results by going to Analyze > Measure or by opening the Results window directly.

• General considerations

 • Scope

 • Exfoliative cytopathology

 • Interventional cytopathology (Biopsy)

 • FNAC

 • Imprint cytopathology

 • Crush smear cytopathology

Introduction
Cytoplasmic male sterility (CMS), a condition under which a plant is unable to produce functional pollen, is widespread among higher plants. CMS systems represent a valuable tool in the production of hybrid seed in self-pollinating crop species, including maize, rice, cotton, and a number of vegetable crops. Hybrids often exhibit heterosis, more commonly known as hybrid vigor, whereby hybrid progeny exhibit superior growth characteristics relative to either of the parental lines. CMS systems can be of considerable value in facilitating efficient hybrid seed production.
Male-sterility-inducing cytoplasms were recognized in the 1900s. Bateson and Gairdner reported male sterility in flax in 1921, was inherited from the female parent, although genes passed from both the male and female parents affected its expression. Chittenden and Pellow (1927) recognized that male sterility in flax was due to an interaction between the cytoplasm and nucleus, with the male sterility conditioned by the homozygous recessive nuclear genotype in combination with the sterility-inducing cytoplasm.

Dark reactions: It is light independent reactions occurs during daytime. This includes C₃, C₄ and CAM pathway involving the utilization of NADPH and ATP to fix CO₂ into carbohydrates. It is also called as stromal reactions.

Day neutral plants: The plants that flowers regardless of the length of the period of the light that is exposed to. They are called as day neutral plants.

DCMU (Dichlorophenyl dimethyl urea or diuron): An herbicide that inhibits non-cyclic photophosophorylation specifically blocks the electron flow at the quinone acceptors of PSII, by competing for the binding site of plastoquinone that is normally occupied by PQB.

DCPIP (Dichlorophenol indophenols): A dye often used as artificial hydrogen acceptor to demonstrate in Hills reaction in isolated chloroplast in laboratory. The oxidized form is blue in alkali and red in acid, while reduced form is colourless.

Dactylis glomerata L (syn. Bromus glomeratus (L.) Scop. (Poaceae): Eng.- Orchard Grass; Kash.- Dramun [W]. Perennial bunch grass,usually in dense shaded forest areas. A very important pasture and hay grass of temperate grasslands. Culms glabrous, erect. Inflorescences are erect panicles bearing spikelets in dense one sided clusters.

Data Analysis: In the process of data analysis, connections or differences supporting or clashing with unique or new hypotheses should be subjected to statistical tests of significance to decide to what validity data can be said to show any conclusions.

Dahi: It is the product obtained from pasteurized or boiled milk by souring, natural or by harmless lactic acid or other bacterial culture. It is also called Curd (Plate 209).

Dairy: It is a room, building, or establishment where milk is kept and butter / cheese is made or it is the department of farming / farm that is concerned with the production of milk, butter, and cheese or an establishment for the sale / and distribution of milk and milk products.

Dairy husbandry: It deals with care, breeding, feeding, milking of the diary cattle and the production and sale of the milk. It is the economical management of a dairy, including looking after the dairy cattle breeding and proper disposal or sale of milk and milk products.

Dairy products: These are the milk preparation, including butter, cream, cheese, ghee, yoghurt, pasteurized milk, tonned milk, butter milk etc processed and prepared in dairy. Ice cream and kulfi or sweets are not included in this.

Damage: Damage is a physical spoilage, often a partial deterioration, are subjectively judge and very difficult to measure and usually reported as per cent damage.

Daminozide: It is a growth regulator which is used for red colour development in apple.

Dahi: Fermented milk product popular in India. Dahi made from buffalo milk is generally preferred to that made from cow milk. A sweet variety of dahi, misti dahi, is prepared by adding cane sugar to milk during heating, giving a caramelized flavour and brown colour.
Daidzein: One of the two isoflavones of particular importance in soybeans, the other being genistein. Both compounds are structurally similar to oestrogenic steroids and possess both oestrogenic and antioestrogenic activities, the principal functions responsible for the health benefits associated with consumption of soybeans and soy products. Dairies/Dairy Factories: Premises in which dairy products are manufactured. It is called creameries or dairy factories.
Dairy beverages: Drinks based on milk or other dairy products, e.g. whey.
Dairy desserts: Ready to eat desserts based on dairy products, such as cream, milk or yoghurt. It is available as chilled, frozen and shelf-stable products. It includes mousses, custards, milk puddings and ice cream products.
Dairy products: Products manufactured from milk. It includes as major product groups, cheese, yoghurt, butter, cream, fermented milk, ice cream and whey products. It is also called milk products.

8.1 General Description

Based upon ethnic values and culture, both private and common property land tenure regime is found in existence in the shifting cultivation system. In Odisha state of India, shifting cultivation locally called as podu, there exists both private and common property regime (Dash 2006). In case of Meghalaya state, while the private land ownership pattern does exist among the Khasi and Karbi tribes, the Garo tribes, on the other hand, resort to community land ownership pattern (Deb et al., 2013). Furthermore, an ethno-agrarian economy based on shifting cultivations is self-sufficient, and such shifting cultivators typically rely on outside sources for alleviation of their economic demands very infrequently, if at all (Gupta 1994). The use of human labour is the key input in the shifting cultivation system and the practice of mutual exchange of labour amongst these shifting cultivator communities is also not uncommon. In practices like harvesting, the groups of families join hands together to harvest the crop of each plot one by one. This practice of community labouring reduces drudgery and increases work productivity. During this harvesting time, it is said that farmers exchange their innovations and look at each other’s innovations as they work. Further, it is customary to arrange food and drinks by the family of the plot which is being harvested. The community recognizes these harvest periods as festive events which makes their work enjoyable. The case of village named Nawalparasi, Nepal, shows that shifting cultivators work in an innovative way in their community in which the part of the village land is set aside and is cultivated by the members of whole community jointly. The benefit they harvest from this area is used to fund for teachers’ salaries in community school (kerkhoff and Sharma 2006). Further, the close knit and kinship ties are not only necessary for labour sharing but also important for technology diffusion among the members (NAEB 2008). The division of labour exists between men and women. It varies from place to place. The Manipur shifting cultivation characteristics portray that the women predominate in seed selection and planting, weeding and other operations, the activities like cutting of the trees in jungle, clearing, burning are generally done by men Both men and women participate in harvesting of produce and so harvested produces are carried to the villages by head loading.

Abstract

Dactylorhiza hatagirea (D.Don) Soo, a plant species native to Indian Himalayas (Badola and Aitken, 2003; Ved et al., 2003), is one of 110 orchids known for their medicinal properties (Jain 1991; Lal et al., 2004). Known earlier as Orchis latifolia auct. non Linn., D. hatagirea has traditionally been used in indigenous system of medicine in India as ‘Hatajari’ or ‘Salam Panja’. The plant has therapeutic, ornamental and ecological significance (Gupta et al., 1998; Vij et al., 1992; Kala, 2000a). Due to over extraction from nature for medicinal use, the species has become threatened and categorized as critically endangered species (Ved and Tandon, 1998; Kala, 2000b; Ved et al., 2003; Bhattarai et al., 2014). This contribution provides insight into the botanical background, distribution, medicinal importance, threat status, cultivation and commercial aspects, and strategies for its conservation and sustainable utilization.

1. Introduction

Milk and milk products constitute essential dietary components for a vast majority of the population. India with annual production of more than 135.6 million tonnes has emerged as leading milk producing nation. The per capita per day availability of milk in India is more than 290 g. Buffalo milk accounts for 57% of the total milk produced in India. Ethnic dairy products account for 90% of all dairy products consumed (Aneja et al., 2002). Many health benefits have been attributed to fermented dairy products and probiotic microorganisms (Salminen et al., 1998). The market for functional foods, nutraceuticals, wellness foods and beverages is fast growing. The harmful effects of synthetic drinks have propelled health conscious consumers to go for natural and traditional drinks. In search of better returns, the Indian dairy industry is widening its focus to include traditional milk products, and these are emerging as new profit centres for the organised sector.

Traditional dairy products have several advantages. They require low capital investment and give high profit margin. They are more appealing to the Indian palate. Dahi or curd is the most popular indigenous fermented dairy product. There are numerous references to dahi in vedic literature. The Prevention of Food Adulteration Act (Anon, 1976) describes dahi as a product obtained from pasteurized or boiled milk by souring (natural or otherwise), using harmless lactic acid or other bacterial cultures. Indian dahi and its western counterpart yoghurt differ considerably in terms of cultures used and their bouquet and flavour profiles (Aneja et al., 2002). The major flavor compound in dahi is diacetyl. Dahi made from buffalo milk has a firm body and white glossy appearance, with a cream layer on top. Mixed mesophilic cultures of Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp diacetylactis along with Leuconostoc species are grown together for production of dahi.

Dahlia (Dahlia spp.) syn. Georgina belongs to family Compositae (Asteraceae). Dahlia was named after “Professor Andreas Dahl”, a Swedish pupil of Linnaeus and author of Observations Botanicae. In India, dahlia is grown as potted plant and landscape uses but it is a poor crop as commercial flower due to shorter commercial life and its potentialities have not been exploited so far. Therefore, there is need to originate more and more new quality cultivars. Dahlias are half-hardy perennials with tuberous roots. Stems are mostly erect, branched, glabrous or scabrous. The leaves 1-3 pinnate, with slightly serrated margin are produced opposite at each node on the stem. They bear flowers on a long, stiff stems well above the foliage. Ray florets are neutral or pinnate and disc florets perfect.

Dahlia (Dahlia spp.) syn. Georgina belongs to family Compositae (Asteraceae). Dahlia was named after “Professor Andreas Dahl”, a Swedish pupil of Linnaeus and author of Observations Botanicae. In India, dahlia is grown as potted plant and landscape uses but it is a poor crop as commercial flower due to shorter commercial life and its potentialities have not been exploited so far. Therefore, there is need to originate more and more new quality cultivars. Dahlias are half-hardy perennials with tuberous roots. Stems are mostly erect, branched, glabrous or scabrous. The leaves 1-3 pinnate, with slightly serrated margin are produced opposite at each node on the stem. They bear flowers on a long, stiff stems well above the foliage. Ray florets are neutral or pinnate and disc florets perfect.

ORIGIN AND DISTRIBUTION

The wild species of Dahlia are natives of Mexico and Central America, where some hybridization and selection had probably already occurred prior to their introduction into Europe at the end of the 18^th century (Crane et al., 1947 and Sorensen, 1969). The dahlia has been in Europe for over 200 years. It came from Mexico to the Botanical Gardens in Madrid towards the end of the 18^th century and was named by Abbe Cavanille in honour of Andreas Dahl, Swedish scientist-cum-environmentalist. The dahlia was introduced into England in 1803, but from a few varieties it soon had more than 300 varieties within the first 15 years. The initial named species imported into Europe were D. pinnata, D. rosea and D. coccinea. The first dahlias grown outside of Madrid were single (open-centred) and multi-ray open centre flowered, but it was not long before the horticultural growers of the day discovered the dahlia was a natural hybrid and when grown from seed, it readily changed its form and colour, Thus it has a range of different types. Further introduction, accompanied by hybridization and selection, have resulted in the production of a wide range of flower, forms and colours. Most of the garden types have been developed from D. variabilis. The first double flowered varieties were called Show and Fancy types. The show were self-coloured, ball like flowers, while the Fancy ones were multi-coloured.

During the mid 1800’s, these Show and Fancy flowers attained cult status with gardeners and several thousand different varieties were recorded. Other forms followed, in 1829 the first Anemone flowered dahlia appeared and then in 1850 the first Pompon was raised in Germany and was named after the bobble on a French sailor’s hat. The origin of the cactus and Decorative type belongs to the arrival of ‘Juarrezii’. According to the written records it was important as a piece of tuber from Mexico in 1872 to Holland. M. Van de Berg of Uttrecht received this so called species from Mexico and released stock of the variety in 1874. Subsequently, ‘Juarrezii’, named after a President of Mexico, was introduced into the UK by W. Cullingford a Vice President of the NDS and distributed by H. Cannell after 1880. Collerettes are the last form of dahlia to have been raised, that has their origin in France and is due to sporting of dahlias at Jardin Botanique de Lyon at the end of the 19^th century.