eChapters

Amino acids are organic molecules made up of carbon (C), hydrogen (H), oxygen (O), and nitrogen (N). However, sulfur (S) and selenium (Se) are also found in some cases. Amino acids are essential organic compounds that come together to create proteins, thus making them the fundamental building blocks of proteins. These biomolecules play vital roles in various biological and chemical processes within the human body and are essential for the growth and development of an individual.

Amino acids consist of an acidic group (-COOH), a basic group (-NH2 ), and a side chain (R) or a hydrogen (H) atom, all bonded to the carbon atom. The features of amino acids are due to the side chain because they differ only in their side chain. All amino acids have the same basic structure except proline, which has a cyclic structure.

Various forms of amino acids like alpha (α), beta (β), gamma (γ) or delta (σ) form are found. The position of the amino group (-NH2 ) determines the form of the amino acid, i.e., if the NH2 group is attached to the α-carbon, it is called α-amino acid; if β-carbon, it is called β-amino acid, and so on. Mostly, α-Amino acids are found as the numbering of amino acids is done from the carboxylic group (-COOH).

Botany: Emblica officinalis belonging to the family Euphorbiaceae commonly known as Amla or Indian Gooseberry or Nelli is an important crop with high medicinal value. The fruits have the richest source of vitamin-C (700 mg per 100 g of fruits) and is considered to be good liver tonic. The various preparations using Amla include Chyavanprash, Triphala churna, Brahma Rasayana and Madumegha churna. The fruit is valued as an antiscorbutic, diuretic, laxative, antibiotic and anti-dysentric. Phyllemblin, obtainedfrom fruit pulp has been found to have mild depressent action on central nervous system. It has good demand from the industries for the preparation of various health care products also like hair oil, dye, shampoo, face creams and tooth powder.

Soil: Light as well as medium heavy soils except purely sandy soil is ideal for amla cultivation. The tree is well adopted to dry regions and can also be grown in moderate alkaline soils.

Climate: It is a tropical plant. Annual rainfall of 630-800 mm is ideal for its growth. The young plant upto the age of 3 years should be protected from hot wind during May-June and from frost during winter months. The mature plants can tolerate freezing temperature as well as a high temperature upto 46^OC.

Varieties: The varieties recommended for cultivation are Banarasi, Chakaiya, Francis, NA-4 (Krishna) NA 5 (Kanchan), NA-6, NA-7, NA-10 and BSR-1 (Bhavanisagar).

Fish excrete Ammonia, is a byproduct from protein metabolism and bacteria break down organic waste such as discarded food, faeces, dead planktons, sewage, and so on. When ammonia is unionized form (NH3) is extremely dangerous; yet when ammonia ionized form (NH4 +) it is harmless; both forms are known as total ammonia. Fish suffering from ammonia toxicity often”become lethargic and frequently emerge near the surface gasping for oxygen. common effects of ammonia in the range >0.1mg/l include Gill damage, breakdown of mucous-producing membranes, “sub-lethal” effects like reduced growth, poor (FC) feed conversion, and reduced disease resistance at concentrations lower than lethal concentrations, osmoregulatory imbalance, and kidney failure.
Principle
When Ammonia react with alkaline Nessler reagent (K2HgI4 or 2KI + HgI2) it produces a yellowish-brown coloured compound. Pretreatment with ZnSO4 and NaOH precipitates Ca, Mg, Fe and sulphide, which form turbidity and apparent colour. Addition of ethylene diamine tetraacetic acid, EDTA (before nessler’s reagent) or Rochelle salt solution prevents precipitation of residual Ca and Mg in presence of the alkaline Nesslers’s reagent.

DNA and RNA are the carriers of genetic information in all the living organisms. The study and analysis of the genetic code can provide information that can be used in clinical diagnostics, drug discovery, genetic engineering and forensic sciences. Hence there is need to understand the techniques in isolation of DNA and RNA followed by their amplification and sequencing.

Polymerase Chain Reaction (PCR) is a laboratory technique, developed by Kary Mullis in the 1980s, used in molecular biology to amplify and replicate a specific segment of the DNA (Mullis, 1990). This reaction in performed in a thermal cycler, involving three main steps: denaturation, annealing and extension.

Principle: PCR is based on the principle of selectively amplifying a specific region of DNA through a series of temperature-controlled cycles. The key components involved in PCR include the target DNA sequence, oligonucleotide primers, DNA polymerase enzyme (Taq DNA polymerase), deoxyribonucleotide triphosphate (dNTPs) (A, T, G, and C), Mg2+ and a thermal cycler apparatus. Initially, the double-stranded DNA is split into single strands under high temperature of 90-95 0C known as the denaturation step. It is followed by cooling the solution to a temperature that allows proper attachment of the oligonucleotide primers to its complementary sites in the strands, known as the annealing step.

Introduction

The AFLP amplified fragment polymorphism also known as selective restriction fragment amplification (SRFA) technique is used to visualize hundreds of amplified DNA restriction fragments simultaneously. The AFLP band patterns, or fingerprints, can be used for many purposes, such as monitoring the identity of an isolate or the degree of similarity among isolates. Polymorphisms in band patterns map to specific loci, allowing the individuals to be genotyped or differentiated based on the alleles they carry. produces highly complex DNA profiles by arbitrary amplification of restriction fragments ligated to double-stranded adaptors with hemi-specific primers harboring adaptor-complementary 5' termini.The technique has been widely used in the construction of genetic maps containing high densities of DNA marker loci.

The AFLP protocol amplifies restriction fragments obtained by endonuclease digestion of target DNA using “universal” AFLP primers complementary to the restriction site and adapter sequence. However, not all restriction fragments are amplified because AFLP primers also contain selective nucleotides at the 3' termini that extend into the amplified restriction fragments. These arbitrary terminal sequences result in the amplification of only a small subset of possible restriction fragments. The number of amplified fragments can therefore be “tailored” by extending the number of arbitrary nucleotides added to the primer termini. Alternatively, the use of endonuclease combinations that vary in their restriction frequency can also be used to tune the number of amplicons. Generally, the abundant restriction fragments produced from complex genomes require of AFLP primers with longer selective regions. Conversely, analysis of small genomes require of only few arbitrary nucleotides added at the primer 3' termini. The resulting AFLP fingerprints are usually a rich source of DNA polymorphisms that can be used in mapping and general fingerprinting endeavors.

Introduction

The AFLP amplified fragment polymorphism also known as selective restriction fragment amplification (SRFA) technique is used to visualize hundreds of amplified DNA restriction fragments simultaneously. The AFLP band patterns, or fingerprints, can be used for many purposes, such as monitoring the identity of an isolate or the degree of similarity among isolates. Polymorphisms in band patterns map to specific loci, allowing the individuals to be genotyped or differentiated based on the alleles they carry. produces highly complex DNA profiles by arbitrary amplification of restriction fragments ligated to double-stranded adaptors with hemi-specific primers harboring adaptor-complementary 5' termini.The technique has been widely used in the construction of genetic maps containing high densities of DNA marker loci.

The AFLP protocol amplifies restriction fragments obtained by endonuclease digestion of target DNA using “universal” AFLP primers complementary to the restriction site and adapter sequence. However, not all restriction fragments are amplified because AFLP primers also contain selective nucleotides at the 3' termini that extend into the amplified restriction fragments. These arbitrary terminal sequences result in the amplification of only a small subset of possible restriction fragments. The number of amplified fragments can therefore be “tailored” by extending the number of arbitrary nucleotides added to the primer termini. Alternatively, the use of endonuclease combinations that vary in their restriction frequency can also be used to tune the number of amplicons. Generally, the abundant restriction fragments produced from complex genomes require of AFLP primers with longer selective regions. Conversely, analysis of small genomes require of only few arbitrary nucleotides added at the primer 3' termini. The resulting AFLP fingerprints are usually a rich source of DNA polymorphisms that can be used in mapping and general fingerprinting endeavors.

Amputation of Claw in Cattle

Amyloid

• Amyloid (G. Amylon; Amyl(o) - STARCH) means starch-like.

• Amyloid is a pathologic glycoprotein deposited in the extracellular spaces and forms fibrils on polymerization.

Histological characteristics

• Amyloid is specially stained with Congo Red.

• Under polarized light, green birefringence is noticed because of alignment of fibrils.

• Amyloid fibrils are 7.5 to 10 nm in diameter, rigid, non-branching hollow-cored tubules of unknown length.

• β-pleated sheet configuration is seen in X-ray diffraction.

• The P-component which is a glycosa-amino-glycan (GAG) facilitates polymerization of amyloid.

An Abstract on Production Technology of SpicesAn Abstract on Production Technology of Spices and Plantation Crops

Veterinary anatomy is a backbone as well as basic discipline in the veterinary sciences at large. The new entrants of the first professional year of B.V.Sc. & A.H. get more exposure in gross anatomy, histo- architect models and developmental anatomy of their current curriculum in our country.
Laboratory demonstration plays a vital role module in virtual teaching and learning program of their better understanding of the subject. The visualization and memorization is the basic requirement for thorough understanding of the anatomy of the individual in medical sciences. The various method of teaching have been debated because it has own limitations. To improve the anatomical understanding the traditional way of using cadavers but with current scenario of restrictive laws which implemented by Veterinary Council of India (VCI) and other organizations viz, SPCA, PFA, CPCSEA/IAEC etc. The scarcity of specimens which are invited the other alternative way of keeping and preserving the specimens for laboratory teaching. Under the unusual circumstances, the normal conventional method of cadaver/specimen/organ preservation should be replaced by the advanced technologies for easy and interesting to learn. In the traditional curriculum method of anatomical specimens taught and learned by formalin fixed cadavers/specimens/tissues. Since, the conventional method creates health problem due to extensive use of formalin as preservative chemical for cadaver preservation. This method of preservation causes wet slippery tissues, respiratory irritation, topical allergic reactions, and students should wear gloves to protect themselves from the fixative like formaldehyde (act as a potential carcinogenic agent). However, the anatomists have been challenged for an alternative laboratory teaching aids in near future. In recent past, the alternative method has developed teaching materials by the innovative novel techniques like Plastination, corrosion cast, pneumatic air dried specimen etc.

Abstract
Coastal and marine ecosystems are important for climate change mitigations. The coastal ecosystems of mangroves, seagrass meadows and tidal marshes mitigate climate change by sequestering carbon dioxide from the atmosphere and oceans at significantly higher rates, per unit area, than terrestrial forests. Phytoplankton are vital autotrophic organisms in marine and freshwater ecosystems, playing a central role in carbon sequestration through the biological carbon pump. By converting atmospheric CO2 into organic carbon via photosynthesis, phytoplankton sustain aquatic food webs and facilitate carbon transport through the food web to the ocean floor as particulate organic carbon. This process, influenced by microbial activity and the sulfur cycle, results in the long-term storage of carbon in deep-sea
sediments, reducing atmospheric CO2 levels. Notably, phytoplankton exhibit high carbon fixation rates and lipid productivity, particularly among the chlorophyceae group, enhancing their effectiveness as a natural carbon sink. Biological carbon fixation is an attractive strategy for climate mitigation, offering a net-zero carbon emission approach to CO2 capture and storage. Assessing the efficiency of phytoplankton in carbon sequestration, through  factors like biomass, growth rates, and oceanic conditions, is essential for understanding and potentially amplifying the ocean’s role as a carbon sink, which is critical in addressing global climate change.
Keywords: Phytoplankton, Carbon sequestration, Climate change and Coastal waters.

Introduction

Value chain analysis has gained considerable popularity in recent years. Although many definitions are applied, value chains essentially represent enterprises in which different producers and marketing companies work within their respective businesses to pursue one or more end markets. Value chain participants sometimes cooperate to improve the overall competitiveness of the final product, but may also be completely unaware of the linkages between their operation and other upstream or downstream participants. Value chains therefore, encompass all of the factors of production including land, labor, capital, technology, and inputs as well as all economic activities including input supply, production, transformation, handling, transport, marketing, and distribution necessary to create, sell, and deliver a product to a certain destination.

ABSTRACT

A low molecular weight antifungal protein has been purified from the leaves of a medicinal herb, Mentha viridis, L. using a combination of cold acetone precipitation, carboxymethyl cellulose column, Q-sepharose column chromatography, and preparatory native PAGE techniques. The purified protein migrated as a single band on SDS-PAGE, with a molecular weight of ca 15.5 kDa. The purified leaf protein of M. viridis inhibited mycelial growth of phytopathogens such as Fusarium solani, Fusarium sp., and Pythium aphanidermatum. The purified protein from the leaves of M. viridis inhibited the growth of human pathogens such as Candida albicans and C. tropicalis (75 mg/mL). This inhibition is concentration dependent. The purified antifungal protein of leaves of M. viridis did not show protease, chitinase, and b-1, 3 glucanase activity.

Millets are an essential food in the world’s semi-arid and arid regions. These are effective energy providers. They provide dietary fibre, polyphenols, fatty acids, minerals, vitamins and protein. The production of tropical crops is highly risky due to the pest outbreaks and increased abiotic pressures triggered by the climate change. Despite the fact that conventional breeding methods have greatly enhanced crop yield and output, new strategies are needed to further enhance crop productivity in order to fulfil the world’s expanding need for the food. The use of site-specific nucleases like zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN) and clustered regularly interspaced short palindromic repeat associated proteins 9 (CRISPR-Cas9) are the genome editing technologies developing very fast. The advancement of plant genome research and its use in plant breeding are significantly aided by such achievements. CRISPR/Cas9 and related genome editing methods are emerging as appropriate methods to reduce the negative consequences of climate change and assure the long-term food security of tropical countries’ growing populations.

Summary

Human being is dependent on nature and natural resources to combat diseases from time immemorial. Ayurveda describes the use of traditional herbs in medicinal preparations in ancient India. Such types of evidences are there in classical Chinese and Greek records also. Nowadays, modern tools are used to identify and purify the active principle from the herbal composition having the therapeutic effect. Bioinformatics tools are often used to screen the drug efficacy of natural products. Using computational and Bioinformatics tools, the efficacy of lead molecules can be screened. Moreover, drug targets can be identified using Bioinformatics and proteomics approach. QSAR and molecular docking tools are used to study the probable activity and mode of action of drug molecule. This approach has minimized the cost and time involved in the drug discovery process.

Abstract

In semiarid climates, a significant component of recharge to basin aquifers occurs along the mountain front. In this scenario Mountain Front Recharge study has been carried along the foothills of Courtallam ,Tamil Nadu. A total of 53 groundwater samples were collected along the foothills and measured for major cations and anions using standard procedure. Saturation index (SI) of carbonate minerals like Aragonite,Magnesite, Calcite and Dolomite,LogpCO₂ and Ionic strength obtained from WATEQ4F output. pH plays a significant role in the saturation index of the minerals. Most of the Carbonate minerals shows undersaturation  with the pH range 7.5. Positive relationship between SI and HCO₃ is noticed. A sharp increase of pCO₂ values towards the foothills region suggests that the groundwater has been recharged by, acquiring longer flow path during its infiltration into deeper level near foot hill region. The SI of these carbonate minerals was spatially distributed to identify the areas of higher SI and also it is compared with the water level fluctuation to understand the recharge process along the foothill region as well as riparian zone.

reach out their fingers it is gone” - Chinese Philosopher Lao Tzu Tao Te Ching. True to his verses, geohazards are phenomena regulated by the law of natureand it is beyond the human intellect to understand thoroughly and capability to conquer. This is the lesson learnt by us from so many incidences that geohazards cause greatest havoc wherein natural processes are going on and are inhabited by humans. Greed for natural resources and population pressures on the earth’s resources are the causes of many a catastrophic event. Examples in this regard are galore including, damning river courses and location of urban centers along rivers and coasts – flooding, cyclonic storms, tsunami hazards; alteration and deforestation hill slopes for agricultural and construction activities – landslides; unscrupulous utilization of ground water resources – drought, etc. This chapter is an attempt to draw the attention of human communityasa whole toturn towards learning to live with the nature without alterning the natural processes to get rid of impacts of geohazards as a best ever mitigation measure. In other words, this chapter attempts to emphasize the need to change our attitude to escape from natural hazards instead of attempting to tinker around natural processes only to get battered at the end.

INTRODUCTION

Oxford English dictionary states that the word ‘disaster’ is derived from the 16^th century French word ‘disastre’, which is a combination of two terms namely, ‘Des’ meaning bad or evil and ‘astre’ means star. Thus “desastre” signifies a ‘bad star’ or ‘evil star’. Disaster, therefore, implies loss or damage occurring due to some unfavorable star. However, in terms of modern knowledge, the word ‘disaster’ denotes any odd event, whether natural or man made, which can bring about sudden and great miseries to humanity in the form of loss of life or/ and property.