eChapters

1. INTRODUCTION

Food additives have been used for centuries in food processing practices such as smoking and salting of meat. Prior to the advent of refrigeration, food grown in the summer had to be preserved for the winter. Salt, sugar, and vinegar were commonly used preservatives. Additives serve many roles and their common uses includes maintaining product consistency and palatability, providing leavening or controlling pH, enhancing flavour and imparting colour. A food additive can be defined in many ways. The Codex Alimentarius Commission, which develops international regulatory guidelines for food additives, provides the following definition of a food additive:

"Any substance not normally consumed as a food by itself, and not normally used as a typical ingredient of the food, whether or not it has nutritive value, the intentional addition of which to food for a technological (including organoleptic) purpose in the manufacture, processing, preparation, treatment, packing, packaging, transport or holding of such food results, or may reasonably be expected to result, (directly or indirectly), in it or its by-products becoming a component of or otherwise affecting the characteristics of such food. The term does not include contaminants or substances added to food for maintaining or improving nutritional qualities".

F.A.O and W.H.O in 1956 defines food additives as non-nutritive substances added intentionally to food, generally in small quantities, to improve its appearance, flavour, or storage properties. The definition did not include vitamins and minerals. Food Safety and Standard Authority of India (FASSI) in 2011 defines food additives “as any substances which is added to food for a technological (including organoleptic) purpose in the manufacture, processing, preparation or treatment of such food and affects the characteristics of such food, but does” not include contaminats or any substance(s) added to the food maintaining or improving the nutritional values. Food additives are those added intentionally to foods and are not naturally a part of a food but can be a part of food unintentionally.

Introduction

Oils and fats are utilized both for their food applications as well as for industrial purpose. As a food product, oils and fats are consumed in a variety of forms such as shortening, ghee, butter, margarine, cooking oil, salad oil, etc. Other uses include their usage in the form of lubricants, greases, soaps, cosmetics, paints as well as animal feed and biofuels. Among the Asian countries, Indonesia leads as the prime producer with more than fifty percent of the total palm oil production in the world. As a second-best producer of oils and fats, China was the major manufacturer of rapeseed as well as soybean oil in year of 2018. In general, Asian countries contributed to more than fifty percent of the total fat and oil manufacturing in the world in 2018 (Kushairi et al., 2018). Even though fats and oils are primarily utilized for food applications, there has been an increased interest in their industrial utility for the manufacture of biofuels. Fats manufactured from various animal sources like tallow or lard also contribute to a considerable quantity and compete with vegetable oils for the production of biofuels.

The resources for producing oils and fats are diverse depending on their end use, yet they are obtained from two major sources: animal sources and plant sources. Oils and fats are used as starting materials for the preparation of shortenings, margarines, and other tailored products that further act as functional ingredients in food products prepared by food processors, restaurants, and in home. They have been used for food and other variety of applications since the prehistoric times, due to their easy availability. For example, fatty tissues from animals liberate free-floating fats on being boiled, whereas oil can be pressed out from oil fruits and oilseeds.

The role of dietary oils and fats in human nutrition is also one of the important areas of interest. Over the time, oil and fat industry has provided significant contributions toward nutritional improvement of various products. With the growing concern linked between cardiovascular diseases and consumption of oil and fat products, low-calorie variants of different foods, free or low in cholesterol are also available now. Excessive fat consumption among the populations of developed countries and consequences of obesity has led to the development of reduced-fat and very-low-fat variants of foods.

Morphological characteristics

Abroma is a shrub or small tree that grows to a height of around 3-5 metres. The plant has horizontal and velvety branches that are 10-30 cm long, 6-18 cm wide, oblong or lanceolate, more or less cordate, delicately acuminate, membranous, whole, and three to five lobed with a petiole that is 1-12 cm long. The dorsal surface of the leaves is glabrous, while the ventral surface is hairy.

Floral characteristics

The plant has beautiful and intricate scarlet like flowers (5 cm in diameter) bearing purple colour. The inflorescence is a cyme. The sepals are persistent and lanceolate, measuring about 2.5 cm long. The petals are imbricate and will shortly fall off. Stamens are situated on a short staminal tube, and there are five staminodes. Each cell in the ovary is five lobed and pyramidal, with numerous ovules. The apex, the capsules are obpyramidal, membranous, finely pubescent, and truncate at the apex. Behind each carpel is a triangular wing. December and January are the fowering and fruiting months.

ABSTRACT

Diabetes is a chronic endocrinologic disorder characterized by high blood levels of glucose due to insufficient secretion of insulin by the pancreas or improper utilization of insulin by target cells. More than 120 million people from all over the globe are estimated to suffer from this malady. Many plants and their constituents have become chief ingredients of a number of pharmaceutical preparations used against diabetes. An intensive survey undertaken for over 4 years in the tribal belts of Orissa to identify the plants used in folk medicine and the methodologies employed by the herbal healers have revealed that 35 species are being employed to cure diabetes. Out of 313 patients (185 males and 128 females) interviewed, 211 (67.5%) use Gymnema sylvestre, Momordica charantia, Trigonella foenum-graecum, Tinospora cordifolia, Pterocarpus marsupium, Andrographis paniculata and Rauwolfia serpentina in one form or the other regularly. Multi-plant preparations gave 80% results whereas single plant administration gave less than 50% results. Polyherbal treatment is advocated as multi-pronged attack to achieve desired results.

Diabetes mellitus is a chronic metabolic disorder characterized by persistent hyperglycemia due to impaired insulin production, insulin resistance or both. It affects various animal species, including dogs, cats, horses and occasionally other animals like birds and ferrets. This condition requires lifelong management and, if left untreated, can lead to severe complications such as ketoacidosis, neuropathy and organ damage. Understanding diabetes mellitus in animals, its clinical manifestations and appropriate management strategies is essential for improving the quality of life for affected animals.
Types of diabetes mellitus in Animals
There are two primary types of diabetes in animals, analogous to human
diabetes:
1. Type 1 diabetes (Insulin-dependent):
Most common in dogs,
Characterized by the destruction of pancreatic beta cells, leading to insufficient insulin production,
Typically immune-mediated or idiopathic,

Animal models in diabetes research are very common when most of the existing models are developed as a conventional model either for Type 1 or for T2D. But very often a conventional model of diabetes cannot demonstrate the specific pathogenesis of diabetes related complications. Therefore, the necessity of the individual and specific model for diabetic complications has been raised in the recent years to achieve the authentic outcomes of specific research aims. A number of animal models of diabetic neuropathy have been developed in last few decades approaching from diverse point of views. However, most of them did not receive much popularity because of their considerable number of limitations and disadvantages. In a comprehensive review, Harati [4] reported that the major handicap in studying diabetic neuropathies is the lack of a suitable animal model that addresses acute and chronic events leading to diabetic neuropathy. Hence, in this review, the pathogenesis, advantages, disadvantages, and limitations of several genetic and nongenetic animal models of diabetic neuropathy have been discussed to substantiate their efficacy for human study and in order to guide diabetes research groups to more accurately select the most appropriate models to address their specific research questions.

Collection of faecal samples: To diagnose gastro-intestinal parasites of ruminants or other animals, the parasites or their eggs/larvae must be recovered from the digestive tract of animal or human or from the faecal/stool material.
 
The faecal samples for parasitological examination should be collected from the rectum of animals. If rectum samples cannot be obtained, fresh faecal samples may be collected from the pastures. Several samples should be collected and immediately dispatched to the diagnostic laboratory in suitable containers such as:

Veterinarian’s primary responsibility towards patients and their owners is to establish a correct diagnosis as it is a pre-requisite for rational, effective, eco-friendly and cheap treatment. Diagnosis of a disease is the crux of health problems. An accurate and prompt diagnosis helps in deciding effective treatment options, whereas incorrect and delayed diagnosis often leads to failure in disease outcome.

Diagnosis

The word “diagnosis” is derived from the Greek word “gnosis” that means knowledge. The diagnosis is not only the identification of the disease but also of disease process correlating with clinical manifestations with an understanding of disease etiology and its influence on the structure and function of the body. In fact making diagnosis is itself a process of ascertaining the nature of the disease or disorder from its signs and symptoms differentiating it from similar looking conditions.

1 Diagnosis of a disease includes

a) Determination of specific cause

b) Abnormality of structure and functions produced by causative agent

c) Clinical manifestation associated with the abnormality

d) All of the above

The Central Nervous System not only consists of the Brain but also involves the Spinal cord.

The spinal cord is responsible for conveying information from the Brain to the rest of the body via the peripheral nervous system. The spinal cord can be divided into 4 functional segments.

1. C1-C5 (Upper Cervical Segments)

2. C6-T2 (Brachial Plexus)

3. T3-L3 (Thoraco - Lumbar)

4. L4-S3 (Lumbo-Sacral Plexus )

It is important to note that the motor and sensory function of the pelvic limbs are not only controlled by the Lumbo-Sacral plexus but also involve the Thoraco Lumbar segments (T3-L3) of the spinal cord. It is important to understand not only the neuroanatomy, but also the functional anatomy before approaching a neurological patient.

Introduction

India is the second largest producer of fruits crops in the world but productivity of arid fruit crops especially in western Rajasthan is miserably low.The productivity as well as the quality of fruit crops is affected to a greater extent due to various physiological disorders. The extremes of environmental variables like changing environmental conditions such as temperature, moisture, unbalanced soil nutrients, inadequate or excess of certain soil minerals, extremes of soil pH, poor drainage and deficiency of micronutrients as well as macronutrient causes many more disorders (Kumar and Kumar, 2015). At present, the climatic scenario of arid region is also changing and the problems like frost, temperature fluctuation, moisture fluctuation, high evaporation and low precipitation as well as nutrient imbalances are becoming a challenge for cultivation of many arid fruit crops. Reasons of low productivity in hot arid ecosystem of western Rajasthan are primarily physiological or stress related disorders such as styler-end browning in ber, fruit cracking in bael and pomegranate, internal necrosis in aonla and black and white nose in date palm. The world wide approximately 70% of crop yield reduction is the direct effect by abiotic factors (Acquaah 2007). In this chapter, we discussed the problems associated with ber, pomegranate, aonla, date palm & bael especially physiological disorders which will certainly help the farmers in timely overcoming of these maladies for getting satisfactory remunerative prices from arid fruit crops under hot ecosystem of western Rajasthan.

Abstract

Bacterial diseases of solanaceous crops like tomato, brinjal, chilli, capsicum and potato are the most serious and destructive diseases affecting both under field and greenhouse conditions. Under moist field conditions they can cause epidemics to affect total crop loss, quality of the produce and reduce market value. These diseases can be distinguished from each other on the basis of their symptoms appearance of the various parts of the plant as well as by using advanced techniques of diagnosis. Diagnosis of the disease is important step to detect pathogen at early stage of disease and subsequently manage it effectively in efficient manner.

Keywords: Bacterial diseases, solanaceous crops, symptoms, diagnosis.

Introduction

Bacterial diseases of solanaceous crops like tomato, brinjal, chilli, capsicum and potato are the most serious and destructive diseases affecting both under field and greenhouse conditions. Under moist field conditions they can cause epidemics to affect total crop loss, quality of the produce and reduce market value. These diseases can be distinguished from each other on the basis of their symptoms appearanceofthe various parts of the plant as well as by using advanced techniques of diagnosis. Diagnosis of the disease is important step to detect pathogen at early stage of disease and subsequently manage it effectively in efficient manner. Therefore, the early detection of the bacterium from asymptomatic plants, soil, and water and plant residues could facilitate elimination and certainly reduce the risk of crop loss. Powerful tools for the identification and detection of the bacterium in diverse substrates (plant, seed, water and soil) are available for diagnosis of the bacterial diseases. DNA amplification of pathogen offers many advantages over classical techniques; neither purification nor cultivation of the pathogen is required and the specificity, sensitivity and response time of tests are improved. Nevertheless, the PCR method has not yet become a routine diagnostic tool for many laboratories, mainly because of the inhibition of the amplification reaction by compounds contained in crude bacterial extracts, which give false negative results or low detection sensitivity.

Cancer has gained considerable relevance in companion animals nowadays owing to the increased awareness among the people towards animal sufferings and pain. A proper diagnosis augments a better treatment regime for the suffering animal. Of all species, dog develops cancer twice as frequently as humans. Mammary tumours rank second (behind skin tumors) as the most common neoplasm in dogs but constituting more than 50% tumours in bitches. All suspected growths therefore requires careful appraisal lest it be cancerous. Diagnosis of cancer represents one of the major challenges faced by the small animal veterinarian. In majority of instances, the differentiation of a benign from the malignant tumour can be made morphologically with considerable certainty and for the same, histopathological examination remains the gold standard. A proper histopathological diagnosis however depends a lot on the collection, fixation and processing of tissues. So clinician as well as histopathologist has their own role to play in the final diagnosis of cancer in animals.

A thorough history with complete physical examination including careful palpation of the mammary glands should be performed. As there are chances that dogs may be suffering from any other systemic ailments during middle to old age. In dogs with multiple tumors, all tumors should be excised and their location and size should be noted. Histopathological examination of the excised tumors should be carried out to classify mammary gland tumors. Mammary tumors are classified based on some of the parameters i.e., tumor size, lymph nodes, metastasis. Therefore, to stage a dog with mammary tumors information of these parameters needs to be collected and recorded. If lymph nodes are palpable and/or clinically enlarged, fine-needle aspiration should be performed for cytological evaluation. The technique is less invasive but with higher accuracy. Thoracic radiographs remain the mainstay for diagnosing the metastatic condition. The mammary tumors most commonly metastasize into lungs in dogs. The staging of CMTs becomes necessary as it is likely to confer some prognostic significance and provide guidance to veterinarians to make treatment decisions.

The speed with which changes in the eye can take place is challenging. Ophthalmic problems do not lend themselves to casual examination or ‘trial and error’ therapy. Therefore, to perform safe cataract surgery with good quality outcome, uniform minimum standard of diagnostic and clinical practice needed to be in place.

Although cataract can be diagnosed by routine gross examination ofthe affected dog even then it is mandatary to develop a routine ophthalmic examinations in a consistent and organized manner to make a confirmatory diagnosis and plan the surgical technique for a favourable prognosis. A sequential examination starting right from history up to electroretinography may be performed as under:

History » Gross Examination » Menace response test » Obstacle course test » dazzle or photic blink reflex » Light induced pupillary reflexes (direct/ consensual) » Corneal reflex test »Schirmer’s tear test

Objective: To identify drought tolerant genotypes by PEG method in laboratory.

Background information:

Among different abiotic stresses, drought is considered to be most detrimental for agricultural production. A drought is a period of below-average precipitation in a given climatic region that results in prolonged shortages in water availability, whether atmospheric, surface water or ground water. A drought can last for several months or years. Drought has substantial impact on ecosystem and agricultural productivity.

Some plant species are better able to tolerate drought stress than others. Genetic variability for drought tolerance also exists within many plant species (Blum, et al. 1980). The efficient exploitation of that variability for crop improvement would be facilitated by a fast and accurate screening method for identifying drought tolerant genotypes (Clark and Mc Craig, 1982). Several methods utilizing seedlings to screen for drought tolerance have been developed using an osmoticum such as polyethylene glycol (PEG) (Singh, et al. 1984). The other synonyms of PEG are Poly ethylene oxide (PEO) and Poly oxyethylene (POE); these other chemicals depend on the molecular weight. Chemical structure of PEG may be written as H”(O”CH₂”CH₂)_n”OH (Kahovec, et al. 2002). PEG is used in certain abiotic stress experiments to produce high degree of osmotic pressure, normally in the order of 'tens of atmosphere' PEG produces osmotic pressure due to its flexible nature and water soluble polymer. PEG also makes certain specific interactions with bio-molecules. Due to above properties PEG is used as a specific chemical to produce osmotic pressure in several biochemical and bio-membrane related experiments. For some genotypes, the seedling response apparently is a good indicator of the plant's reaction to drought stress later in its life cycle. However, early screening of seed plants via seedlings tests must still be considered as a method for identifying genotypes that will tolerate drought stress in the vegetative stage (Andersen, et al. 1987).

1. California mastitis test

The California mastitis test (CMT) is conducted during milking of lactating cows. The CMT paddle is used to collect about three to four ml of fore milk from each quarter of cows after discarding few streams to which an equal volume of CMT reagent (composition mentioned below) is mixed immediately by circular motion (Fig. 1). The reaction is graded by intensity of gel formation and color changes as follows:

   Q.1.    How milk sample is collected for diagnosis of mastitis?
    Ans.    At first the end of teat is cleaned with a cotton swab dipped with 70% alcohol, then the dirt and wax are removed from the external sphincter by pressure. After this milk sample is collected in screw cap bottle.

    Q.2.    When milk sampling should be done in suspected cases of tubercular mastitis?
    Ans.    Last few streams of milking is crucial in cases of tubercular mastitis.

    Q.3.    What is the effect of freezing of milk sample on bacterial count?
    Ans.    On freezing of milk, Arcanobacter pyogenis and E. coli counts are decreased, coagulase negative bacteria counts increases whereas, Streptococcus and Staphylococcus spp. counts may be unaffected or increased.

    Q.4.    Which media is suitable for initial isolation of bacteria from milk samples?
    Ans.    Blood agar. However for isolation Streptococcus agalactiae selective media is required

DSM-5 and Diagnosis of Mental Health Issues
Mental health is a fundamental aspect of overall wellbeing and the accurate diagnosis of mental health issues is crucial for providing appropriate and effective treatment. The Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM-5) is the latest reference guide used by mental health professionals to diagnose mental health conditions (Kapadia et al., 2020). The current edition, DSM- 5, builds on the goal of its predecessors of providing guidelines for diagnoses that can inform treatment and management decisions. The DSM-5 provides a comprehensive and standardized framework for the classification and diagnosis of mental disorders.
The DSM-5 represents a significant advancement in the field of psychiatry, as it incorporates a cross-cultural assessment of mental health issues and an updated classification system. (Petito et al., 2020) This detailed diagnostic criteria helps clinicians accurately identify and differentiate mental health conditions, enabling them to develop tailored treatment plans.

Diagnosis of Poisoning    
Three types of diagnosis
 a) Tentative
 b) Presumptive
 c) Confirmative based on the following conditions
 1. History
 2. Physical examination and clinical evidences    
Hyperthermia- Organochlorine;    
Hypothermia- OP compunds    
Miosis: Opium 
Mydriasis- Belladona    
Dryness of mouth (Xerostomia)- belladona;    
Moist and Hypersalivation- OPC, Carbamates and synthetic pyrethroids 
Bitter Almond    smell-Cyanide
Garlic odor in expired air- Phosphorus, Arsenic    
Mice smell- Hemlock    
Rotten Garlic/Horse raddish smell- Selenium Poisoning

• General considerations

 • Tuberculosis

 • Johne’s disease

 • Brucellosis

 • Infectious bovine rhinotracheitis

 • Campylobacteriosis

 • Tichomonosis

Introduction

Diagnostic biochemistry, is a branch of laboratory medicine that focuses on the analysis of bodily fluids to diagnose and monitor diseases. This field plays a crucial role in understanding the biochemical mechanisms underlying various conditions, enabling accurate diagnosis, prognosis, and management. The biological samples for diagnosis include blood, urine, cerebrospinal fluid, pleural fluid and tissues.

Factors Affecting Serum Enzyme Activity or Protein Level

1. Organ Mass and Enzyme Tissue Concentration:

• Higher enzyme concentrations in organs can lead to greater increases in serum enzyme activity during disease. For instance, the liver’s large mass contributes to potential increases in serum ALT activity.

2. Cell Location

• The proximity of cellular enzymes to the blood, urine, or other fluids determines the fluid in which an enzyme’s activity will increase upon cell injury. For example, renal tubular gamma-glutamyltransferase (GGT) is released into urine, not blood, following injury.

Diagnostic biomarkers are measurable biological molecules that provide information about physiological or pathological conditions. This chapter examines the use of metabolomics for discovering, evaluating, and validating biomarkers for disease diagnosis, prognosis, therapeutic monitoring, and personalized medicine. It discusses different types of biomarkers, metabolomic biomarkers, biological samples, analytical platforms, biomarker-discovery workflows, and statistical analysis. Applications in cancer, diabetes, cardiovascular, liver, kidney, neurological, infectious, and metabolic diseases are considered. The chapter also explains the role of metabolomics in drug discovery, toxicology, nutrition, and clinical research. Challenges involving biological variability, analytical reproducibility, data complexity, and clinical validation are addressed alongside future opportunities involving AI and multi-omics.

Diagnostic techniques are routinely used for diagnosing different surgical conditions. The most frequently used imaging techniques in veterinary practice are radiography and ultrasonography. Radiography and ultrasonography are not suitable for each organ in large animals. In small animals most organs are suited to ultrasound images except bones and gascontaining structures such as lungs etc. Bone and gas reflect ultrasound and do not allow the sound waves to form image therefore radiography gives better diagnostic images for these organs. The liver, spleen, kidneys, prostate, gravid uterus and fluid containing organs like urinary bladder, gall bladder and major blood vessels can be better imaged with ultrasound. In buffaloes in surgical section important clinical conditions relate to urinary and digestive systems, mammary gland and perineal area.

1. Urinary System: In bovine, the cases of obstructive urolithiasis are commonly observed. These types of cases are more common in bullocks and in buffalo calves. In these cases radiography is not so useful. Sonography can be tried to take the image of ruptured urethra in bullock and can be compared with radiography. Due to ruptured urethra there will be accumulation of urine in the s/c tissue of prepuce. Due to fluid density the image will be radioopaque and will appear white and no detail of the organ can be seen. The ultrasonogram (USG) will show images of glans penis and its coverings. Different diameters of urethra and a point of rupture just behind to glans penis can be make out. Due to obstruction, The B.calves either will pass drop wise urine or there will be complete stoppage of the urine. These cases can also be easily diagnosed by ultrasonography.

The aquaculture sector in India is expanding rapidly, playing a critical role in enhancing rural livelihoods, ensuring food security, and boosting export revenue. However, disease outbreaks remain a persistent hurdle, often leading to substantial financial losses for fish farmers. To mitigate this, the deployment of user-friendly, efficient, and rapid diagnostic tools at the field level has become increasingly vital—especially in areas with limited access to laboratory facilities.
Fish disease diagnostic kits, based on immunological and molecular technologies, are designed to detect specific pathogens responsible for viral, bacterial, parasitic, and fungal infections. A major benefit of these tools is their ability to deliver results swiftly—typically within minutes or hours—enabling prompt action. In India, research bodies such as ICAR-CIFA, ICAR-CIBA, and ICAR-NBFGR have played a key role in developing and adapting these tools for local aquaculture conditions.
Efficient nucleic acid extraction is a cornerstone of field diagnostics. Rapid DNA/RNA extraction kits that function without centrifuges and under varied environmental conditions have made sample processing quicker and easier. Mobile diagnostic vans—equipped with portable lab equipment—have also been introduced in states like Andhra Pradesh and Tamil Nadu for on-site disease surveillance and farmer training.
For detecting parasites, wet mount kits containing preloaded reagents like iodine and saline are used for direct microscopic examination of fish tissues. These kits help identify parasites such as Trichodina, Ichthyophthirius, and monogeneans, aiding in immediate diagnosis and treatment advice.

12.1. Introduction

The protocols provides guidance on the structure and content of the International Plant Protection Convention (IPPC) diagnostic protocols for regulated pests. The protocols describe procedures and methods for the official diagnosis of regulated pests that are relevant for international trade. They provide at least the minimum requirements for reliable diagnosis of regulated pests.

This standard sets the framework for the content of diagnostic protocols, their purpose and use, their publication and their development. Diagnostic protocols for specific regulated pests are included as annexes to this standard. Information relevant for diagnosis is provided in the diagnostic protocol on the specified regulated pest, its taxonomic position, and the methods to detect and identify it. Diagnostic protocols contain the minimum requirements for reliable diagnosis of the specified regulated pests and provide flexibility to ensure that methods are appropriate for use in the full range of circumstances.

The methods included in diagnostic protocols are selected on the basis of their sensitivity, specificity and reproducibility, and information related to these factors is provided for each of these methods. Detailed information and guidance for the detection of pests is provided on, for example, signs and/or symptoms associated with the pest, illustrations (where appropriate), developmental stages of the pest, and methods for detecting the pest in a commodity, as well as methods for extracting, recovering and collecting the pests from plants.

Introduction

To maintain optimum reproductive status early diagnosis of reproductive problems is important. Various advance diagnostic techniques are available for diagnosis of reproductive problems. Some of them are still in early stages of development or some though available; are not widely used due to various constrains.The various diagnostic techniques used in animal reproduction are ultrasonography, Doppler ultrasonography, endoscopy, laparoscopy, hormonal assays, vaginal cytology, x ray etc.

Accurate history and Thorough examination of reproductive tract

Accurate history is a powerful tool of enormous clinical value. Behavioral problems due to pain or psychological factors can also be ruled out on the basis of adequate history of the patient. Tentative differential diagnosis can be made by taking history of previous breedings.Through digital and vaginiscopic exanimation of reproductive tract helps to rule out anatomical abnormalities, which may interfere with natural mating. It also helps in diagnosis of fertile phase of oestrus cycle. In cases of refusal to mate due to anatomical problems like septum, narrow passage or due to behavioral problems AI can be performed. Vaginoscopic examination aids vaginal cytology in deciding right time of natural service or insemination in proper breeding management or abnormal oestrus cycle.

Any abnormal condition, which affects the normal functioning of cells, tissues, organs and any other system in an individual, is called a disease. In all living organisms, including plants, animals, birds and humans, diseases can be caused due to infectious or non-infectious agents and various other factors. Laboratory tests may identify organisms directly (eg, visually, using a microscope, growing the organism in culture) or indirectly (eg, identifying antibodies to the organism). Some tests (eg, Gram stain, routine aerobic culture) can detect a large variety of pathogens and are commonly done for many suspected infectious illnesses. However, because some pathogens are missed on these tests, clinicians must be aware of the limitations of each test for each suspected pathogen. In such cases, clinicians should request tests specific for the suspected pathogen (eg, special stains or culture media or advise the laboratory of the suspected organism(s) so that it may select more specific tests. The main causative factors of diseases in humans include internal factors, genetic irregularities, allergies, poor immune system, external factors and a lot more.

Several diagnostic techniques are employed to asses the nutrient status of the crop. These are helpful in determining specific or multinutrient stresses and quantity of nutrients needed to eliminate the stress. Some of the techniques commonly used are:

Visual deficiency symptoms

Plant analysis

Rapid tissue testing

True /False

Q.1.    A diagnostic test enables the doctors to reach at a decision whether the patient is suffering from a particular disease or not.
Q.2.    The recent developments in the field by biotechnology have led to the development of many new medical tests.
Q.3.    The general principles in designing and conduct diagnostic test studies are not known well.

X-ray Machines
 
There are many factors to consider when choosing X-ray equipment. The need of an individual practice varies depending on the species majority and case work load.
Types of X-ray machines: 
X-ray machines can be grouped into 3 categories:

I. Portable X-ray Machine

    ·    Is one that can be carried to the animal.
    ·    Portable X-ray machines are widely used in veterinary practice.
    ·    The maximum output is 15 to 35 mAs and kV ranges from 70-110 kVp.
    ·    Cost is less and easy to move around and transport. 
    ·    Suitable for X-raying of thin and small parts (limbs below stifle and elbow of large animals and can be used taking 
X-rays of abdomen an skeletal systems of small animals).

Section-A : Write True / False

Q.1 A better and more beautiful way to present the data in a more visual form can be done by means of diagram.

Q.2 Description of data through diagrams and graphs is better for layman.

Q.3 Diagram does not register a meaningful impression almost before we start to think about it.

Q.4 Diagrams are never used by the business man and government agencies for advertisement and educating people.

Q.5 Diagrams do not give visual presentation of data.

Q.6 The diagrams put a rememberance for a longer time in mind of human being.

Q.7 Diagrams facilitate comparison and reveal pattern of data.

Q.8 Diagrams show approximate values of items in data.

Q.9 Diagrams are more attractive, appealing and impressive than tables.

Q.10 Diagrams can be more easily compared and interpreted than the tables.

Q.11 Tables contain precise figures while diagrams indicate approximate figures.

Diagrams and graphs are the effective and attractive ways of presenting data. Widely used diagrams for data presentation are pi chart and bar diagrams. Bar diagrams are also of various types; (i) Simple bar diagram (ii) Component or sub-divided bar diagram (iii) Multiple bar diagram (iv) Percentage bar diagram. The widely used graphical presentation are histograms, frequency polygon and frequency curve.

There are numerous libraries to create charts and graphs in R programming language.

Pie-chart- A pie-chart is a representation of values of variable as sectors of a circle with different colours/shadings. The colours/shadings used for different sectors are indexed at the side of the graph. pie() function is used to create pie chrt in R.

Let us create an exel file names as ‘exp_sports’ which contains the expenditure made (in Rs.) under various sports in a sports club. The name of the sports are cricket, hockey, tennis and football. The names of sports are placed under the column head ‘sports’. The corresponding expenditure (in Rs.) are 7200, 3600, 1800, 1800 and they are placed under the column head ‘expenditure’.

The basic syntax for creating a pie-chart using the R is.

Diagrammatic Presentation of Data

The quantitative data when classify are converted into averages and if the number of average is large then it is not possible to draw a conclusion from their large numbers of averages. Moreover quantitative data are dull, confusing, boring for many of us. Therefore, a statistical device to make such confusing data more attractive was developed i.e. if such complex data are presented by geometric f igures, pictures, graphs or curves then it will become more attractive and more impressive, is called as diagrammatic representation of data. These diagrams are nothing but the use of geometrical figures to improve the overall presentation and offer visual assistance for the reader.

Types of Diagrams

Generally, diagrams are made for a special series or a time series and they are divided into five different categories:

1) One dimensional diagrams

2) Two dimensional diagrams

3) Three dimensional diagrams

4) Pictograms

5) Cartograms

4.1 Diagrammatic Representation of Data

Diagrams are meant only to give a pictorial representation of the data with a view to make them readily intelligible. Several types of diagrams are used for the presentation of data in biological and agriculture sciences. The following are the important types of diagrams in common use :

1. One-dimensional diagrams
2. Two-dimensional diagrams
3. Three-dimensional diagrams

Introduction
Plant breeding, according to Sansern et al. (2010), is the process of genetically altering plants to meet human demands. Prehistoric humans in various parts of the world domesticated only a small number of the hundreds of thousands of species that were then available. They did this by genetically altering these species’ components, which entailed both conscious and unconscious selection as well as differential reproduction of variants (Allard, 1999). A relatively small number of plant species have evolved over many years of trial and error to become the backbone of agriculture and, consequently, the world’s food supply (Mackay, 2001). This method of domestication includes the identification of specific valuable wild species along with a system of selection that resulted in alterations in the quality and quantity of cultivated crops.The modifications brought about by domestication involved altered organ size and shape, loss of many survival traits, such as bitter or toxic substances, the disintegration of seeds in grains protective structures, such as spines and thorns, seed dormancy, and change in life span increased for crops grown for roots or tubers and decreased for crops grown for seed or fruit. The domestication process followed mostly by mass selection (selection by bulking preferred kinds) is a potent method for facilitating quick changes while preserving genetic variability in the population (Okporie and Obi 2002).
Populations can exist in constantly changing surroundings due to genetic variation. More variation increases the likelihood that certain individuals in a group will have allele variants that are appropriate for the environment. Those individuals have a higher chance of survival to have offspring which carry that allele. Because of their success, the population will endure for additional generations. Selecting the best parents to utilize in artificial crosses is one of the key concerns for plant breeders since it encourages the expression of the largest genetic variability and the production of superior recombinant genotypes. The objective of plant breeding is to simultaneously improve all the desired traits. This task is complicated by correlations between various traits, which may result from pleiotropic genes, physical chromosomal links between genes, or population genetic structure. Choosing one feature will alter associated qualities, sometimes in a positive or in a negative way. As a result, selection may result in unexpected modifications, provided they fall within the typical range of variations seen in the crop and are therefore thought to be useful to consumers or the environment. Breeding practices in the 20th century improved a variety of genetic techniques, including methods for identifying and preserving more genetic diversity, creating variable populations for selection, and testing how to differentiate between the effects of genes and the environment. Naturally self-pollinating plants like soybeans or tomatoes, naturally cross-pollinated plants like maize, and plants whose vegetative propagation (typically cross-pollinated) allowed the direct fixing of improved types, all required different precise steps in the production process (Ubi, 2013).

Dialysis
 
The main purpose of dialysis in biological research is the removal of small molecular weight (M.W.) constituents from biological fluids. This is achieved by dialysing the material in a cellulose tubing with a pore size of 40-80 A0 (4-8mm) diameter which allows the passage of compounds with molecular weight less than 10,000. The following is the procedure followed: cut the length of the dialysis tubing depending upon the volume to be processed. Open the sides of the tubing with a flow of distilled water and transfer it to a boiling water bath. Do not allow the tubing to dry as it will distort the pore size. Make a bag by tying a knot at one end of the tubing. Transfer the solution to be dialysed into the bag. Tie another knot at the top of the bag. The bag is then put in a beaker containing the solution against which dialysis is to be carried out. In general, one volume of the desired solution is dialysed against 50-100 volumes of buffer. The total period of dialysis could range from 8-48hr. The efficiency of dialysis could be increased by stirring and changing the dialysate solution at frequent intervals.

Key Points

• Neonatal diarrhea can be lethal without treatment.

• Administer aggressive fluid and electrolyte therapy to address newborn diarrhea.

• Fecal examination and/or culture findings can help control diarrhea.

• Ringer lactate is the preferred fluid.

• There is a high association between animal age and the presence of the causal factor for diarrhea.

Introduction

Diarrhea is one of the symptoms of a variety of medical problems. Diarrhea is characterized by an increased frequency of defecation and stools of varied consistency, which may have an increased concentration of water or a decrease in dry matter content. Increased peristaltic movement causes an increase in caudal flow of ingesta/GIT content, resulting in a decrease in intestinal transit time and thus diarrhea. Diarrhea is lethal in children; therefore, preventing f luid and electrolyte losses prior to diagnosis is critical to saving lives.

Many non-spore-forming, anaerobic, Gram-negative bacteria cause opportunistic mixed infections, often in association with facultative anaerobes. Synergistic interactions between the organisms in these mixed infections are common. Fusobacterium species and bacteria formerly referred to as Bacteroides species account for more than 50%of the anaerobic organisms isolated from these infections.
Usual Habitat
Nan-spore-forming, Gram-negative anaerobes are often found on muwus membranes, particularly in the digestive tract, of animals and man. They are excreted in the faeces and they can survive for short periods in the environment. Dichelobacter nodosus, a primary pathogen of the epidermal tissues of the hoof region of ruminants, survives for less than 4 days in mud. Diagnostic Procedures
In order to ensure that isolates of anaerobes are aetiologically significant, specimens for isolation procedures should be obtained by direct sampling from discharges or lesions and by supra pubic puncture in urinary infections. Specimens should be processed promptly after collection. Commercial kits and transport media are available for specimens from suspected anaerobic infections. In the core of a tissue specimen over 2-3 cm, an anaerobic microenvironment is usually maintained. Samples of fluid in a syringe remain suitable for anaerobic culture if air is expelled from the syringe and the needle
is plugged.
Anaerobic jars with an atmosphere of hydrogen and 10%CO2 are used for incubating cultures at 37°C for up to 7 days.

8.1 Introduction to Dictionary in Python

A dictionary is a collection which is unordered changeable and index. In python dictionaries are written within curly brackets, and they have keys and values. Means the dictionary contains two things first is the key and the second is the value.

8.1.1 Create and Print a Dictionary

Example

dict1={‘brand’:‘Suzuki’,‘model’:‘dzire’,‘year’:2020}

print(dict1)

Accessing items: you can access the items of a dictionary by referring to its key name, inside square brackets.

Example

Accessing items: there is also a method called get() for the same task.

dict1={‘brand’:‘Suzuki’,‘model’:‘dzire’,‘year’:2020}

print(dict1)

y=dict1.get(?model”)

print(y)

Abstract

The electromagnetic processing instruments such as microwave is very commonly used at house hold level, hence understanding the behavior of food as well as factors affecting its dielectric properties becomes important. Dielectric properties (DEP) play an important in electromagnetic processing of food products and mainly depend upon the moisture content, physical properties, composition, temperature and power applied. Different methods have been used to determine the DEP of food material including parallel plate, coaxial probe, resonance cavity, transmission line and free space method. The selection of these methods is again based on several factors such as type of materials, accuracy of measurement, the scope of the research, frequency range, availability of equipments and resources. Present study thus summarizes the importance of DEP of food, influencing factors and the different measurement techniques.

Diet plays an important role in the management of diseases. It is very much true in the management of dogs with heart failure also. Managing the diet of a dog with heart failure is depending on the severity and stage of heart failure and presence / absence of concurrent diseases as well deficiencies.

Introduction

Fiber is that part of plant food which is not digested when eaten. Since it is not absorbed into the body, dietary fiber is not a nutrient. Nevertheless, fiber is a very important component of a healthy diet. Fiber, also called “roughage” helps prevent constipation, hemorrhoids and diverticular disease. Some types of fiber have a cholesterol-lowering effect, which could lead to reduced risk of heart disease.

Dietary fiber has been defined as plant cell wall material resistant to the intestinal secretions of the host. This definition gives the appearance of precision and allows gravimetric and chemical measurements. However, a proportion of other non-fibre dietary constituents (e.g, retrograded starch, protein, and fat) pass along the gut, without absorption or metabolism, to the caecum. Chemical analysis or quantitative measurements of the fibre content of specific diets do not allow prediction of their biological action. The physiological effects of dietary fibre depend predominantly on physical properties unrelated in any simple or direct way to chemical composition. Quantitative measurement of dietary fibre does not allow for diverse effects on nutrient absorption, sterol metabolism, fermentation in the colon, and stool weight. We still do not know whether to restrict the definition of “dietary fibre” to non-starch polysaccharides or to include related materials such as resistant starch, lignin, and the complex assemblies typical of plant tissues, where polysaccharides occur in close association with other molecules. An alternative to defining fibre in terms of chemical composition would be to develop measurements of physical properties relevant to physiological action in the intestine. This approach could be extended to other polymeric constituents, in particular proteins (ingested and secreted) and mucous glycoproteins.

Polysaccharides are the main constituents of dietary fibre and show a wide spectrum of physical, properties that reflect the nature and extent of intermolecular association. Dietary fibre can be likened to a water-laden hydrated mass passing along the gut. Physical properties that influence function are a combination of the rheological and colligative properties of the water-soluble fibre components, the surface characteristics of the water-insoluble components, and the network properties of the swollen hydrated components. Such features include viscosity, water holding, cation exchange, organic acid adsorption, gel filtration, and particle size distribution.

Introduction

In India, per capita cereal consumption has been declining since the early seventies, despite a significant increase in per capita cereal production (Radhakrishna and Ravi, 1992 and Rao, 2000). Cereal consumption per capita per month fell from 15.35 kgs in 1970-71 to 12.70 kgs in 1999-2000 in rural area and from 11.40 kgs to 10.40 kgs in urban area during same period. It might be due to shifting of consumers’ choice from food to non-food items; from cereals to non-cereals within the food group and from course to fine cereals within the cereal group. The decline in cereal consumption has been higher in the rural area due to development of rural infrastructure and availability of job opportunities which ultimately helped in improving their level of income (Rao, 2000). Despite spectacular growth in food production during last 30 years the problem of malnutrition still persists across different regions in India because increase in food grain supply alone cannot conquer hunger and malnutrition.

The consumption pattern and nutritional status have also experienced a significant change during last two decades in Bihar. Cereal consumption per capita per month has declined from 476 gms in 1983 to 455 gms in 19992000. The situation is more alarming in poor households who constitute about half of rural population in north Bihar. There is lack of research on consumption pattern, nutrient intake and nutritional status based on household data, particularly in context of Bihar. In view of the above observations the present study is undertaken to analyze the consumption pattern and nutritional status of poor households in north Bihar.

Abstract

The concept of functional foods has received significant media attention and, as a result, interest has focused on the need for research into the non- nutrient constituents of food plants. This has enabled market penetration of functional foods containing increased amounts of bioactive food compounds or physiologically active nutrients and non-nutrients within food that impart proven health benefits. Functional foods can take many forms. Some may be conventional foods with bioactive components that can now be identified and linked to positive health outcomes. Some may be fortified or enhanced foods, specifically created to reduce disease risk for a certain group of people. Numerous observational studies have shown that omega-3 fatty acids enriched diets are associated with a reduction of cardiovascular mortality, heart attack and sudden death. In several studies, a higher fish intake has been associated with decreased incidence of coronary artery disease (CAD) and cardiovascular mortality. A trial revealed that fish oil supplementation of 130 mg/kg of body weight each day decreased the number of stiff joints, duration of morning stiffness, pain and global arthritis activity. Many supplements contain active ingredients that have strong biological effects in the body. The bioavailability of nutrients within a given food is governed by the composition and microstructure of the food, commonly referred to as the food matrix. More specifically, numerous studies investigating the development of functional foods have shown that the interactions between nutrients and non-nutrients within the food matrix can be synergistic, additive, or neutralizing in nature. The preventive and curative effects of the functional foods on health concludes it to be integral components of established public health programs to reduce the risk of specific diseases.

7.1 Introduction

In this chapter, process, outcome and impact evaluation of the vegetable integrated pest management programme (IPM) implemented in the subtropical area of the Jammu region of Jammu and Kashmir (J&K) state during 2008-09 and 2009-10 was studied. There are many research designs employed for impact evaluation. These include one group before and after comparison design, one group time-series design, non-equivalent control group design and ex-post facto research design. Social scientists most often used before and after IPM (longitudinal) comparisons, with and without IPM, and control group (latitudinal) comparisons, and a combination of the two (longitudinal and latitudinal). A robust measure of the impact of a programme, however, is the difference-in-differences (Ashenfelter and Card, 1985) quasi-experimental design, a statistical technique used in econometrics and quantitative sociology to determine the difference between the treatment (with) and control group (without) over time. The difference-in-differences quasi-experimental design, also called double difference (DD), used to compare the treatment (with IPM) and control group (without IPM) over time. The objective of our quasi-experimental empirical study was to determine the impact of the vegetable IPM programme on the adoption of non-chemical pest management practices, pesticide use, and field use environmental impact in the sub-tropical region of the J&K state. Difference-in-difference quasi-experimental control group design (with/without, before/after) was used to compare the changes before and after the IPM programme between the IPM trained farmers and the non-IPM farmers. The DD model was employed to identify the impact of the IPM programme on the adoption of non-pesticide IPM practices, pesticide use by weight (a.i.), pesticide use frequency, farmers’ knowledge regarding safe use and handling of the pesticides, and field use environmental impact to address the bias introduced by a non-random sampling procedure as the farmers from FFS were selected intentionally (Table 1). The DD model also eliminated the in-built, systematic or seasonal effects other than the training effect.

Pharmacy and Therapeutics Committee
The Pharmacy and Therapeutics (P&T) Committee is a vital component of healthcare institutions, particularly in hospitals. It serves as a formal body that oversees medication management processes, ensuring that drug therapy is safe, effective, and cost-efficient.
Objectives of the Pharmacy and Therapeutics Committee
1. Formulary Management

• Definition: The formulary is a continually updated list of medications approved for use within a healthcare institution. It serves as a guide for prescribers regarding which medications are available and recommended for various conditions.
• Objective: The primary objective of the P&T Committee in formulary management is to ensure that the formulary reflects the most effective and safest therapeutic options based on clinical evidence. This involves evaluating new drugs for inclusion and removing outdated or less effective medications.
2. Drug Selection
• Rationale: The committee aims to specify preferred drugs and alternatives based on clinical guidelines and evidence-based practices. This helps minimize therapeutic redundancies and ensures that healthcare providers have access to optimal treatment options.
• Process: The selection process involves reviewing clinical studies, safety data, cost-effectiveness analyses, and recommendations from professional organizations to make informed decisions about drug inclusion.
3. Quality Assurance
• Importance: Quality assurance is critical for patient safety. The P&T Committee monitors medication use within the hospital to identify trends in prescribing patterns and potential safety issues.
• Monitoring Activities: This includes reviewing adverse drug reactions (ADRs), medication errors, and compliance with formulary guidelines. The committee implements corrective actions when necessary to improve safety outcomes.

Composting means decomposing organic wastes. It is a safe, non-polluting and microbiological method for disposal and recycle of organic wastes by converting those to balanced plant food materials, commonly known as compost, the organic fertilizer. In other words, the mineralization and partial humifi cation of organic materials by the action of microorganisms under suitable condition is called composting (Figure 6.1). The organic wastes undergo different physical and chemical changes with the help of microbes like bacteria, fungi, actinomycetes, etc. During composting the population of pathogenic microorganism reduces while that of benefi cial microorganism increases.