eChapters

Normally raw milk contains different levels of inhibitory substances, which restrict the normal development of bacteria and some times may even kill them. The degree of inhibitory activity varies considerably in milks of different species, viz, cow, buffalo, human. Maximum activity present in human milk, variations occur in the milks of individual animals and some times even at different milking or from different quarters of the udder of the same animal.
There are two groups of antimicrobial substances present in milk:
    1.    Inherent or naturally occurring substances in milk. 
    2.    Non- inherent compounds secreted into milk from other sources.

Antinutrients

Safety is the most important aspect among all the quality attributes of food. Food can be considered safe, only if it does not contain any factor which is hazardous to human or even animal health. The various factors which affect the safety of the foods may be broadly classified into three categories. The first category includes the toxic factors occurring naturally in foods. The second category comprises a group of incidental contaminants which find their way at various stages of processing, manufacture, preservation and storage of foods and the third category includes harmful intentional additives or adulterants.

Various categories of anti-nutritional factors are present in the traditional and non-traditional oilseeds, oils and oilcakes. Some of them are heat-labile and do not pose any harmful effects in the body, while the heat-stable factors may cause deleterious effects in human beings and animals. They belong to different groups of compounds. It is necessary to know their behaviour, significance and toxicities. Their avoidance or elimination is important to protect human and animal health.

ABSTRACT

Free radical scavenging property and antimicrobial activity of Triphala, the herbal product made of equal proportion of Terminalia chebula, Terminalia bellerica and Emblica officinalis were evaluated. Ethanol extracts of the formulation were tested for its invitro antioxidant activity by using improved ABTS radical cation decolorizing assay and invitro antimicrobial activity against Streptococcus mutans (predominantly involved in bio-film formation) by both well-diffusion and turbidity method to determine its minimal inhibitory concentration. An effort was also made to correlate its antiplaque activity using an invitro assay (where conditions kept similar to oral cavity) with Triphala and two commercial toothpastes (Colgate and Pepsodent). The ethanol extract showed significant antimicrobial and antioxidant activity. Triphala showed effective antiplaque activity when compared with the other tooth pastes. The herbal extract effectively inhibited the bio-film formation and more antioxidant activity exhibited by the extract might protect the gum cells effectively from free radicals than the commercial toothpastes, having less antioxidant activity.

Introduction

Vegetables are essentially important functional foods capable of providing additional physiological benefits such as delaying onset of chronic diseases apart from meeting basic nutritional requirements. Vegetables constitute a significant source of carotenoids, phenolics, vitamin C, flavonoids and glucosinolates which boost the immune mechanism and have medicinal properties. The antioxidants in tomato, onions, carrots, garlic, brussel sprouts, peppers and green leafy vegetables and their potential health benefits are critically discussed.

Oxidative damage to biological molecules like DNA, protein and lipids induced by free radicals is considered to be linked with many degenerative diseases such as cardiovascular, cancer, ocular and neurological such as Alzheimer’s and Parkinson’s. Antioxidants from plant sources preserve an adequate function of immune cells against homeostatic disturbances and act as functional foods. Functional foods are foods which besides fulfilling the basic physiological function, have health promoting effect. There is a considerable epidemiological evidence indicating association between diets rich in vegetables and a decreased risk of cardiovascular disease and certain forms of cancer. Diets rich in fruits and vegetables contain high amounts of antioxidant compounds like vitamin C, vitamin E, carotenoids, flavonoids, tannins and other phenolic constituents which boost the immune mechanism.

Abstract

The cereal grains are grown around the world, the most common is wheat, which accounts for one third of the total grain crop worldwide. In addition to providing energy, whole grains contain several key nutrients, including fibers, lipids, B vitamins and minerals as well as other beneficial components such as phenolic acids, flavonoids, lignans and phytosterols. Several of these components function as antioxidants. While multiple studies have been conducted to analyze antioxidants in different grains. In wheat and other grains, naturally occurring phytonutrients with antioxidant activity include phenolic acids, flavonoids, coumarins, polyphenols, phytates, terpenes, carotenoids, tocopherols and tocotrienols. Potential roles of the naturally occurring antioxidants in grains include free radical scavenger, reducing agent, complexation of prooxidant metals, quenchers of singlet-oxygen molecules and antioxidant defense enzyme system activators for biological systems. Wheat grain contains several components that have potential antioxidant activity and thus may have a protective role in human health. Traditional wheat-based breakfast foods include hot and cold ready-to-eat (RTE) cereals, as well as a variety of yeast-leavened and chemically leavened bakery products. Increasing baking time and temperature increases antioxidant activity in the bread crust and lowers antioxidant activity in the bread crumb portion. Wheat and wheat based products, particularly partial and 100% whole grain products have been shown to provide a variety of beneficial components including fiber, vitamins, minerals and other phytonutrients with antioxidant activity. Scientific evidence indicates that consumption of three or more ounce equivalents of whole grain foods per day may reduce the risk of coronary heart disease, type 2 diabetes and certain cancers and help with weight management.

The importance of air in human life is well known as air is required for running different biological processes in human body. Unfortunately, the same life giving air or oxygen can cause harmful side effects like generation of oxidants or free radicals in our body. Reactive oxygen species, by product of respirative cycle of oxidative phosphorylation, are well known to interfere with cytoplasmic function and bio-molecules and for inflicting damages on cellular bio-molecules, which pre-disposes cells to several anomalies like mutagenic aberration, cell ageing, diseases, cancerous tumour growth and development of various lethal diseases such as cardio-vascular diseases, cancer, atherosclerosis etc. (Gorinstein and others 2001; Apak and others 2004). Free radicals disrupt the proper membrane function, denature proteins and inhibit enzyme function. Antioxidants, naturally present in food, play a vital role in trapping the radicals and; therefore, effective in reducing the risk of heart diseases, immune system decline, different inflammatory reactions etc. (Silici and others 2010).

Free radicals are unstable because they are missing an electron and they try to become stable by attaching to cell membranes in the body, snatching away electrons from them. This, in turn, sets free radicle molecules on an electron snatching spree and starts a chain reaction that ultimately leads to cell death. On the other hand, antioxidants (such as carotenoids, anthocyanins, ascorbic acid, flavonols, flavonoids, polyphenols etc.), present in our foods can prevent or slow down the oxidative damage by donating one of their own electrons, ending the electron-stealing reaction. Epidemiological evidences suggests that intake of antioxidants helps in scavenging free radicals, cause of a broad spectrum damages to biological system (Vertuani and others 2002). This has led consumers to look for a diet, derived from natural sources as well as rich in antioxidants as an strategy to counteract the cellular damages wreak by oxidative stress.

1. Introduction
Aquaculture has emerged as a vital sector in global food production, with the increasing demand for fish and seafood as a primary source of protein for a rapidly growing population. According to the Food and Agriculture Organization (FAO), aquaculture contributes significantly to global fisheries production, accounting for nearly 50% of the total fish consumed worldwide. This remarkable growth trajectory is driven by the need to meet food security challenges while providing sustainable and nutritious food sources. Despite its importance, intensive aquaculture practices present numerous challenges that can adversely affect fish health and productivity. Fish raised in aquaculture systems are often subjected to a variety of environmental stressors, including poor water quality, high stocking densities, rapid growth rates, and fluctuations in temperature. These stressors lead to increased metabolic activity in fish, which can elevate the production of reactive oxygen species (ROS) — highly reactive molecules that can cause cellular damage. Under normal physiological conditions, the production of ROS is counterbalanced by the antioxidant defense mechanisms in fish. However, when the production of ROS exceeds the capacity of these defense systems, oxidative stress ensues.
Oxidative stress is a detrimental condition characterized by an imbalance between ROS generation and the antioxidant capacity of an organism. In fish, this condition can manifest in various forms, including cellular damage, lipid peroxidation, protein oxidation, and DNA damage. The consequences of oxidative stress are severe, resulting in impaired growth, compromised immune responses, increased susceptibility to diseases, and ultimately higher mortality rates. Understanding the underlying mechanisms of oxidative stress in aquaculture is critical for developing effective strategies to mitigate its impact and improve fish welfare.

Abstract

Dietary antioxidants can be found naturally in various fruits, vegetables and food products.Typically, antioxidant phytochemicals commonly found in food products are Vitamin C, Vitamin E, carotenoids, polyphenols, melanoidins and indoles. The main characteristic of an antioxidant is its ability to scavenge free radicals and form stable end product which inhibit the chain oxidative mechanisms that lead to degenerative diseases which are harmful to human health. Drying is widely used in food processing to minimize food wastage and to extend food shelf-life. Pretreatment, drying methods and storage conditions are some of the processing parameters affecting the quality of dried products. Selection of processing conditions and storage environment are therefore critical in maximizing the retention of food antioxidants. This chapter aims to compile and summarize studies carried out for antioxidants namely antioxidant contents in dried food products, effect of processing parameters and quantification methods in the analyses of antioxidants.

Antirrhinum is a genus commonly known as snapdragon belongs to family Scrophulariaceae. Snapdragon is one of the flowering plant present with vast genetic diversity in India. The term Antirrhinum is derived from the Greek word “anti” means like and “rhin” means a nose, referring to the snout like shape of the flower. The cultivated Antirrhinum majus is grown in tropical, subtropical and temperate countries. The common name snapdragon, originates from the flowers reaction to having their throats squeezed, which causes the mouth of the flower to snap open like a dragon’s mouth. Flowers are borne on terminal long spikes of many colours except blue and with numerous shades. It is one of the excellent cut flowers which have long lasting qualities. These are also used as garden plants, bedding plants, in rockeries or herbaceous borders and as potted plants. Plants can be grown for cut flowers in an open field or under protection depending on the climate. The leaves and flowers are antiphlogistic, bitter, resolvent and stimulant (Chiej, 1984).

Antirrhinum is a genus commonly known as snapdragon belongs to family Scrophulariaceae. Snapdragon is one of the flowering plant present with vast genetic diversity in India. The term Antirrhinum is derived from the Greek word “anti” means like and “rhin” means a nose, referring to the snout like shape of the flower. The cultivated Antirrhinum majus is grown in tropical, subtropical and temperate countries. The common name snapdragon, originates from the flowers reaction to having their throats squeezed, which causes the mouth of the flower to snap open like a dragon’s mouth. Flowers are borne on terminal long spikes of many colours except blue and with numerous shades. It is one of the excellent cut flowers which have long lasting qualities. These are also used as garden plants, bedding plants, in rockeries or herbaceous borders and as potted plants. Plants can be grown for cut flowers in an open field or under protection depending on the climate. The leaves and flowers are antiphlogistic, bitter, resolvent and stimulant (Chiej, 1984).

ORIGIN AND DISTRIBUTION

The garden snapdragon (Antirrhinum majus) has emerged as a model organism during early studies of inheritance and mutation (Darwin, 1868) because of its diploid inheritance, ease of cultivation and variation in morphology and flower colour. The genus Antirrhinum includes 15 New World species in the section Saerorhinum and 21 Old World species in the sections Antirrhinum and Orontium. A. majus (Snapdragon) is a species of Antirrhinum native to the Mediterranean region, from Morocco and Portugal north to southern France and East to Turkey and Syria. Most species are found around the Mediterranean Sea and in North America (Stubbe, 1966). The progenitor of the modern snapdragon is from the Mediterranean region and more specifically, it is native to southern France. It was believed that the snapdragon reached Britain with the Romans at an early date, where it has been naturalized on mountainous regions. Thereafter, it spread to different parts of the world from Britain.

The scientific name of the genus Antirrhinum was first defined by Carl von Linne in the year 1753. In the year 1914 Professor Erwin Baur the founder of the MaxPalnck-Institut für Züchtungsforschung in Cologne published a volume called ‘Einführung in die experimentelle Vererbungslehre’ (Introduction to Experimental Genetics) 2nd edition. This book contained several plates with reproductions of water colour paintings of snapdragons as examples. He crossed a white line with a red line resulting in a pink flower. This pink flower then was selfed and the offspring showed the typical distribution of 1 white, 2 pink and 1 red as a demonstration of Mendelian inheritance. Antirrhinum glandulous is native of California. In United States of America, winter flowering cultivars were introduced in 1926 by Frank Volz of Cheviot, Ohio. In 1959, snapdragon were included in the Agricultural Censuses of Horticulturally Special Crops and ranked seventh as a cut flower crop.

The genus Antirrhinum offers an inexhaustible resource for genetics-based studies in developmental biology, biochemistry and evolution (Stubbe, 1966). Wide diversity of Antirrhinum species, combined with classical and molecular genetics, the two traditional strengths of Antirrhinum provides an opportunity for developmental, evolutionary and ecological approaches (Schwarz-Sommer et al., 2003). Transposons responsible for flower variegation were identified in the 1980s. The Max-Planck-Institute in Cologne, Germany, allowing genes involved in flower and leaf development and in pigmentation to be isolated by transposon tagging. Population genetic studies that have been applied to Antirrhinum species show different population sizes, geographic distributions and breeding systems, from self-fertility to obligate out-crossing (Jimenez et al., 2002 and Mateu-Andres and de Paco, 2006). It was introduced to India with the advent of British. Among them, only A. majus has been domesticated as an ornamental.

Introduction
Using short, single-stranded oligonucleotide strands complementary to the targeted mRNA, as RNA technology can regulate gene expression, leading to changes in phenotype, disease resistance, and other traits associated with protein expression profiles. Since gene function depends on expression, this technology can be used to silence genes and inhibit their expression. Because it can manipulate and regulate protein expression during its translation, it can knock out or down the expression of a gene of interest. As such, functional genomics can benefit from this technology as it allows for the regulation of protein expression.
Gene expression involves two main steps: transcription, in which the DNA sequence serves as a template to create an mRNA molecule, and translation, in which the mRNA is used as a template to synthesize a protein. The genes in DNA provide the instructions for making proteins, which are essential for the functions of the cell.
Polymer chain that transforms into an effective protein. The triplet code, which forms the basis of the genetic code, designates an amino acid for each codon made up of three nucleotides. Several enzymes, including together, enzymes such as polymerase, exonuclease, ligase, etc., unravel the DNA double helix and match RNA base pairs with DNA bases to create an RNA copy of the original DNA.

Introduction

Casein has been widely used as a substrate for measuring the trypsin inhibitor activity of natural trypsin inhibitors such as those which occur in Soybeans and other legume. The most common method employing casein is the one originally described by Kunitz (1947) which involves the spectrophotometric determination of the breakdown products produced by a given concentration of trypsin in the presence and absence of the inhibitor. On the other hand, the tryptic hydrolysis of a synthetic substrate such as benzoyl-DL-arginine-p-nitroanilide (BAPA), first introduced by Erlanger et al. (1961), does follow a zero order reaction, and one obtains, within broad limits, a linear relationship between the quantity of p-nitroaniline released and the concentration of the active enzyme. Although BAPA has been used for assaying the antitryptic activity of soybean fractions (Sambeth et al., 1967).

Antiviral Factor (AVF)

When an incompatible virus enters the host, resistance to viral diseases is seen. A number of substances, including polyacrylic acid, aspirin, salicylic acid, benzoic acid, and other growth regulators, including Indole acetic acid (IAA), 2, 4-D, Benzyl amino-in (3P), and ethylene, cause resistance to viral diseases. For each of these situations, certain plant compounds have also been identified to cause the development of novel proteins in plant tissues. They are referred to as “antiviral factors.” Healthy plants lack these antiviral components. They are only created once the host has been infected by the virus. These post-infectional substances resemble “phytoalexins.”

Indian gooseberry or aonla (Emblica officinalis Gaertn.), is an important fruit crop of 21st century and it has been regarded as ‘Amritphal’ in ancient literature, and has been identified as an ideal plant for various kinds of wastelands viz, moisture stress, eroded, ravines, upland, riverbed and the areas with undulated topography (Korwar et al., 2006) as it possesses some specific characters like intensive and deeper root system, summer dormancy of zygote, reduced leaf area, synchronization of fruit growth and development with moisture availability period and selective absorption of cations and anions which enable it to grow in fragile agro-climatic conditions. India is principal aonla growing country and Uttar Pradesh is the leading state and Pratap garh district, U.P. is well known for aonla cultivation throughout the country. Aonla is a deciduous tree, small to medium in size and distributed in tropical and sub-tropical forests in India. Looking to its high demand and value added products, now, it is being commercially cultivated in different parts of country. It can tolerate alkalinity and salinity and can be grown in wide range of soils.

Aonla is said to be indigenous to tropical south-eastern Asia, particularly in central and southern India (Firminger, 1947). The genus comprises of about 350 species. It is also called Indian gooseberry, amla, nelli, myrobalan, amlaki, amali, ambala, amlika, sirikai, amalakamu in different regional languages in India. Aonla is one of the richest sources of natural ascorbic acid which is protected by high polyphenols content during storage and processing. Aonla is indigenous to central and southern India. It is found it Sri Lanka, Malaysia, China and Malacca. In India, it is widely cultivated in Uttar Pradesh, Tamil Nadu. Madhya Pradesh, Rajasthan, Gujarat, Chhatisgarh and Jharkhand.

Cultivars/Varieties: Some of the well-known improved varieties are Banarasi, Chakaiya, Krishna (NA-5), Kanchan (NA-4), Hathi Jhul, Neelam (NA-7), Amrit (NA-6), Balwant, Anand-1, 2 & 3.

Uses

The fruit is widely used in ayurvedic preparation due to its pharmacological properties. The fruits are made into preserve (murabba), sauce, candy, dried chips, etc., Fruits are pickled and made into, jelly, toffee, etc., bark and leaves are used for tanning and wood is used for preparing hookah.

Aonla or Indian gooseberry (Emblica officinalis Gaertn.) is one of the most important traditional but underutilized fruits of Indian origin, having immense potentiality of cultivation on marginal and waste lands. It belongs to  family Euphorbiaceae and sub-family Phyllanthoidae. Aonla tree thrives well throughout the tropical and sub-tropical parts of India either found growing wild or cultivated. Aonla is a quite hardy, prolific bearer and highly remunerative even without much care. It can be grown easily on calcareous and slightly saline as well as alkaline soils where common fruit crops do not thrive.
 
Aonla is regarded as sacred by Hindus and has great mythological significance. According to Hindu mythology, one day meal is arranged beneath an aonla tree during Kartik (October) when trees are laden with fruits. Hindu religion also prescribes that ripe fruits of aonla be eaten for forty days after fast  to restore health and vitality known as Kaya Kalp (Benthal, 1946).

ABSTRACT

A long term study was conducted to evaluate different aonla based silvipastoral systems for compatibility, productivity and conservation effects. Three grass species viz. dholu, hybrid napier and perennial arhar were evaluated for 14 years for degraded lands of Shivaliks. Hybrid napier because of its vigour growth adversely affected the growth of aonla but effect on yield turned non-significant. Arhar could survive only during pre-bearing state. Studies on soil losses and runoff for decades showed that Embilica officinalis + Chrysopogon fulvus was the best silvipastoral system for soil conservation. This system saved rainwater and soil in a range of 4.88 to 30.66 cm and 862-2818 kg ha^-1 respectively over sole plantation. The combination of Embilica officinalis + Chrysopogon fulvus proved to be the most effective silvipastoral system for reclamation of degraded lands and improving economic status of people in Shivalik foot hills.

Aonla or Indian Gooseberry (Emhlica officinalis Gaertn. syn. Phyllanthus embfica L.). belonging to family Euphorbiaceae. is an indigenous fruittree to Indian sub continent. It is one of the most important commercial crops suitable for marginal soils and various kinds of waste lands particularly salt affected lands. A lot of genetic variability is available in India with respect to growth and fruit quality characteristics of aonla. Almost all the budded varieties of aonla were selected from naturally occurring superior chance seedlings having in view their earliness, lateness, hardiness to adverse conditions, yield and superior fruit quality.

1-or successful aonla orchards, it is utmost necessary to have basic knowledge of various cultivars suitable for a particular set of environmental and soil conditions.

This crop is gaining importance as a main ingredient in Indian diet due to its medicinal properties. Large areas are under its cultivation and the larger fruited varieties have become very popular. It is one of the prime constituents in ayurvedic preparations
Bark-eating caterpillars - Indarbela quadrinotata, I. tertraonis: Metarbelidae
The larvae of this species make zigzag galleries and feed on the bark under silken ribbon- shaped webs. They are stout and dirty brown in colour.
Fruit- borer : Deudorix Isocrates : Lycaenidae: The larvae of this beautiful butterfly cause considerable damage (up to 42%) to fruits.  They start feeding while the fruit is tender, and growing. During severe infestations, chemical control may be warranted. This can be done by spraying contact insecticides like malathion, or dichlorvos or  fenvalerate or other synthetic pyrethroids.