eChapters

Dry matter is generally defined as the constant  weight a sample  attains when heated at 100ºC (occasionally 150ºC). This definition , suitable for inert materials such as sand, has three main lacunae when applied to biological materials.

    1.    In these materials, water is present in various states ranging from extraneous moisture  as rain or dew, through water present in cell sap, water present in various physico-chemical and chemical combinations.

    2.    Biological materials contain active respiratory enzymic systems which continue to function during the early stages of drying; in fact, as the material heats up, activity will be enhanced until it is stopped either  by denaturation of the enzymes or by desiccation. Besides altering the chemical composition of the material, such activity will  lead to loss of dry matter.

    3.    Most biological materials contain organic  compounds which are volatile at 100ºC and which are, therefore, lost on drying. This leads to an under-estimation of dry matter content, for example, in the case of faeces, silage and molasses.

The term “laboratory” refers to the room in the building specifically equipped for the chemical manipulation of radioisotope. The word “shall” is used to indicate procedure which are mandatory.

    1.    No unnessary materials are to be brought into the laboratory. Eating drinking, smoking and the using cosmetic in the laboratory are specifically forbidden.

    2.    Pipetting or the performance of any similar operation by mouth suction is prohibited.

    3.    Laboratory protective clothing (gloves, smocks, etc.) shall be left in the laboratory; gloves shall be removed before using the counting equipment.

    4.    Before leaving the laboratory, the hands shall be washed first, then checked with a beta-gamma survey meter. Contamination remaining after thorough washing shall be reported to the instructor.

    5.    If, in the course of work, personal contamination is suspected, a survey with a suitable instrument shall be made immediately, to be followed by the required cleansing. Routine precautionary surveys should be made at intervals.

    6.    No person shall work with active materials while having abrasions in the skin on the hands without using rubber gloves.

The term “laboratory” refers to the room in the building specifically equipped for the chemical manipulation of radioisotope. The word “shall” is used to indicate procedure which are mandatory.

    1.    No unnessary materials are to be brought into the laboratory. Eating drinking, smoking and the using cosmetic in the laboratory are specifically forbidden.

    2.    Pipetting or the performance of any similar operation by mouth suction is prohibited.

    3.    Laboratory protective clothing (gloves, smocks, etc.) shall be left in the laboratory; gloves shall be removed before using the counting equipment.

    4.    Before leaving the laboratory, the hands shall be washed first, then checked with a beta-gamma survey meter. Contamination remaining after thorough washing shall be reported to the instructor.

    5.    If, in the course of work, personal contamination is suspected, a survey with a suitable instrument shall be made immediately, to be followed by the required cleansing. Routine precautionary surveys should be made at intervals.

    6.    No person shall work with active materials while having abrasions in the skin on the hands without using rubber gloves.

Answer the followings
    Q.1.    What is mastitis?
    Ans.    It may be defined as inflammation of mammary parenchyma and characterized by physiochemical changes in the milk and pathological changes of the glandular tissue.
    Q.2.    What are the cardinal changes of milk and udder in mastitis?
    Ans.    Changes of milk includes discoloration, presence of clot, and presence of huge leukocytes whereas, mammary gland becomes hot painful swelling and induration.
    Q.3.    What is sub clinical mastitis?
    Ans.    In subclinical mastitis, normally mammary gland is normal by physical examination; however, cellular constitution only indicates presence of mastitis (i.e. increased somatic cell count).
    Q.4.    What is the basis of diagnosis of sub clinical mastitis?
    Ans.    Diagnosis sub clinical mastitis depends largely on indirect tests which are related to leukocyte content of milk.

Answer the followings
    Q.1.    What is the relative incidence of  Streptococcus agalactiae, other Streptococci and Staphylococcus aureus
    Ans.    1:1: 2
    Q.2.    What are sources of contagious pathogen for mastitis?
    Ans.    Udder surfaces of infected quarters 
    Q.3.    What are sources of environmental pathogen for mastitis?
    Ans.    Bedding of cow’s environment
    Q.4.    When animal get infection from environmental pathogen?
    Ans.    Animal get infection at any time during the life such as milking time, between milking, during dry period and prior to first calving.
    Q.8.    What is the probable period of new infection from environmental pathogen?
    Ans.    Early part of dry period and first two months of lactation.
    Q.9.    What is the relation of growth of Corynebacterium bovis in milk and lactation?

Answer the followings
    Q.1.    What is the international organizations associated for making outline the mastitis control programme?
    Ans.    National Institute for Research on Dairying (NIRD) and National Mastitis Council (NMC).

    Q.2.    What is the important objective of dairy industry as regards to mastitis control?
    Ans.    The important objective is to achieve an excellent udder health for production of high quality of milk.

    Q.3.    What is the basic principles of mastitis control programme?
    Ans.    There three basic principles of mastitis control programme:
    a)    Elimination of existing infection
    b)    Prevention of new infection 
    c)    Monitoring of udder health status

Al-Ani, F.K. and Vestweber, J.G.E., 1986. Udder oedema: an update review. Vet. Bull., 56: 763-769.

Aitken, I.D. 2007. Diseases of sheep, 4^th edition, Blackwell publishing, UK.

Bennett, R. H. and Jasper, D. E. 1980. Bovine mycoplasmal mastitis from intramammary inoculations of small numbers of Mycoplasma bovis: local and systemic antibody response. Am. J. Vet. Res. 41(6):889-92.

Bennett, R.H. and Jasper, D.E. 1977. Bovine mycoplasma mastitis from intramammary inoculation of small numbers of Mycoplasma bovis. I. Microbiology and pathology. Vet. Microbiol., 2(4): 341–355.

Bergey’s Manual of determinative bacteriology. (edited by) John, G. Holt Fetd. 9th edn. (1994).

Bottom of Al-Ani, F.K. and Vestweber, J.G.E., 1986. Udder oedema: an update review. Vet. Bull., 56: 763-769.

Bertschinger, H. U., Pohlenz, J. and Hemlep, I. 1977. Studies on the mastitis-metritis-agalactia syndrome (milk fever) in sows. II. Bacteriological findings in spontaneous cases. Schweiz Arch Tierheilkd.119(6): 223-33.

Bostedt, H., Lehmann ,B. and Peip, D. 1988. The problems of mastitis in mares. Tierarztl. Prax.. 16(4):367-71.

Bradley, A. J. 2002. Bovine Mastitis: An Evolving Disease. Vet. J., 164(2): 116–128.

Buchanan, A. G. 1982. Clinical laboratory evaluation of a reverse CAMP test for presumptive identification of Clostridium perfringens. J. Clin. Microbiol. 14:761–762.

Bushnell, R. B. 1984. Mycoplasma mastitis. Vet. Clin. North Am. Large Anim. Pract., 6(2):301-12.

Chareonsirisuthigul, T., Kirpal, G., Amtsberg, G. 1979. Biochemistry, serology, pathogenicity and antibiotic resistance of an Escherichia coli strain from healthy sows and from animals with mastitismetritis-agalactia syndrome. Berl. Munch Tierarztl. Wochenschr. 92(21):409-16.

Christie, N. E., Atkins, N. E. and Munch-Petersen, E. 1944. A note on a lytic phenomenon shown by group B Streptococcus. Aust. J. Exp. Biol. Med. Sci. 22:193–195.

Claxton, P.D. and Ryan, D. 1980. Bovine mastitis bacteriology. Australian Standard Diagnostic Techniques for Animal Diseases. Pp. 1-8

Daley, M. J., Oldham, E. R., Williams, T. J. and Coyle, P. A. 1991. Quantitative and qualitative properties of host polymorphonuclear cells during experimentally induced Staphylococcus aureus mastitis in cows. Am. J. Vet. Res., 52(3): 474-9.

Darling, J. F. 1975. Standardization and evaluation of CAMP reaction for the prompt, presumptive identification of Streptococcus agalactiae (Lancefield group B) in clinical material. J. Clin. Microbiol. 1:171–174.

De, U. K. and Mukherjee, R. 2005.Status of nitric oxide production by polymorphonuclear cells during clinical mastitis in lactating cows. Indian J. Comp. Microbiol. Immunol. Infec. Dis., 26(1): 61-62.

De, U. K. and Mukherjee, R. 2008. Anti-inflammatory and respiratory burst activity of Azadirachta indica during acute bovine mastitis Indian J. Anim. Sci., 79(2): 32-34.

De, U. K. and Mukherjee, R. 2009. Expression of cytokines and respiratory burst activity of milk cells in response to Azadirachta indica during bovine mastitis. Trop. Anim. Health Prod., 41(2): 189-197.

De, U. K. and Mukherjee, R. 2009. Inhibitory response of Azadirachta indica extract on nitric oxide production by milk leukocytes during clinical mastitis. Vet. Arhiv, 79(1), 41-50.

De, U. K. and Mukherjee, R. 2009.Prevalence of mastitis in crossbred cows. Indian Vet. J., 86:858-859.

De, U. K. and Mukherjee, R. 2011. Potential of Indigenous Enzymatic Activities, Nitric Oxide and Ceruloplasmin in Bovine Milk to Diagnose Subclinical Mastitis. J Veterinar Sci Technol 2:109. doi:10.4172/2157-7579.1000109

Introduction

The cereal cyst nematode, (Heterodera avenae Woll.) a causal organism of ‘molya’ disease in wheat and barley, since after its first report (by Wollenweber, 1924, Vasudeva, 1958 and Prasad et al., 1959), it has been timely reviewed about its spread and losses caused in the various parts of wheat and barley growing areas of the world. It has been reported to occur in Australia, Canada, Israel, South Africa, Japan, in most of the European countries, North America, India, Morocco, Tunisia, Pakistan, Libya, Turkey and Australia (Prasad et al., 1959, Smiley et al., 1994, Rumpen horst et al.,1996, Krall et al., 1999, Nicol, 2002 and Rao et al., 2002). Its economic importance has been assessed by mapping the yield losses caused in cereals i.e. ranged from 15-20% in Pakistan, 40-92% in Sauthi Arabia, 23-50% in Australia (Nicol, 2002) and 40-50% in India (Mathur 1969, Mathur et al., 1980, Handa et al., 1985, Mathur, et al., 1987).

The world’s largest collection comprising 1117 entries is maintained at Turkmenistan (CIS). They exhibit great variability for fruit size and quality, skin color, resistance to abiotic and biotic stresses but most of them are temperate in nature and do not grow well in the Indian tropics. There is scope to use this material for breeding superior types.

There are several varieties adopted to both tropical and subtemperate climate. They are either evergreen on semi deciduous or deciduous types. The flowering habit, fruiting and flower physiology are altered with the habitat. The tropical climate with mild winter of south India, growth and flowering are continuous processes, while in the subtropical climate of north India the trees remain dormant during cold winter and flowers in the following spring and in temperate climate flowering occurs in summer.

Irrigation/ Drip/ Fertigation

Pomegranate is considered to be drought hardy fruit crop. Supplemental irrigation, however, is necessary for realizing higher fruit yield and better quality. Irrigation is also used to regulate cropping in pomegranate and the total water applied therefore depends on the desired bahar. In Gujarat and Maharashtra, where Ambe bahar is taken, regular irrigation is required during March to July and irrigation is stopped after the fruit harvest until January. For Mrig bahar crop irrigation is not required from July to September. However, in case of prolonged break in monsoon one or two supplementary irrigation at an interval of 10-15 days during October to December are required. In hasta bahar (September flowering) prolonged rainy season helps in reducing requirement for irrigation 3-4 irrigation at monthly interval after cessation of rainfall are must to obtain good fruit size and higher yield.

High Density Orcharding

India is the second largest producer of the fruits (41.5 million tones) in the world. Still the productivity of almost all the fruits in India is very low (12 tones /ha) as compared to other fruit growing countries of the world. It is a major cause of concern to our scientists, extension personnel and orchardists. Many reasons may be attributed to the low productivity and to solve this problem our scientists have to develop high yielding varieties/hybrids, resistant to the biotic and abiotic stresses and develop better agro-techniques for realizing the maximum potential of the fruit cultivation in India. Though, we have achieved this distinction, but the per capita consumption of the fruits in our country is still 80 g/day due to our huge population, which is far below from the recommendation of 120g/capita/day.

Crop Regulation

Staggering in pomegranate is practiced with a view to regulate crop load on the plant which will help in availability of the fruit for the long period helping the farmer fetching remunerative prices in the market. Also it will help in avoiding the glut in the market leading to distress sale. The relationship between source and sink can be regulated which helps in better fruit development. Pomegranate flowers and fruits through out the year if irrigation is provided to the plant. Taking of particular bahar leads to the glut in the market. This results in distress sale by the farmers. To avoid this staggering of pomegranate crop was attempted to find out and fruiting flowering during various months of the year so that maximum production with best quality fruits can be obtained.

The term arrowroot is commonly applied to many starch producing plants belonging to different species whose economic part is the rhizome, eaten fresh or made into flour. The term applies both to flour and the plant. All the plants of the arrowroot group have underground rhizomes or tubers from which arise reed like erect stems. The word ‘arrowroot’ is derived from the pointed shape of the rhizome or its known ability to cure arrow injuries. The word itself is derived from the carib word araruta meaning mealy roots. The arawak, a people native to the Caribbean islands, considered arrowroot a dietary staple. They called it aru-aru meaning meal of meals.

Coleus, commonly known as Chinese potato, is a minor tuber crop of the tropical regions of India, Indonesia, Malaysia, Sri Lanka and Africa. It is considered as a native of India , where its cultivation is mostly confined to the southern states. In Kerala, it is commonly known as koorka or cheevakizhangu. In Malaysia it is called ubi kemili, kentang kechil, kembili and kumbili java. It is also known as hausa potato, Sudan-potato, country potato, fra-fra potato, fra-fra-salanga, ratala, pomme de terre du Soudan, hausakartoffel, innala, saluga, tumuku and fabirama. 

Nutritive status
The nutritive status of this crop compares favourably with many of the major tuber crops.  Coleus tuber, with its characteristic  flavour has a special preference among consumers. Compared to other tuber crops, it fetches premium price in the market, although it is known as poor man’s potato. The tubers are used for the preparation of various delicious side dishes. Reliable data on area and production of this crop are not available. Despite the high market price and consumer preference, it is not available throughout the year.

Exacum bicolor, Roxb. commonly known as country kreat is  endemic to Peninsular India. It is a herbaceous perennial with attractive flowers and posseses medicinal properties. Plant is endangered today due to large scale habitat destruction.  Non- lodging nature of plant, attractive bi-colored flower bunches with a long flowering period and good field life offer great future for this plant to be domesticated as an attractive garden flower. Its ornamental value and medicinal properties are yet to be fully exploited.

India is bestowed with a wide array of flowering plants. It represents two of the 18 hotspots of biodiversity in the world; one being the Western ghats and other the North Eastern Himalayas. Nature has also blessed our India with  uniqueness of endemism. Of the 17000 flowering herbs distributed in nine phyto geographical regions of the country, 5700 are endemic. Exacum  bicolor Roxb. is one of the endemic species to Peninsular India. Plant is popularly known as Bharachirata and Habshi chirata in Hindi, Kannamthali in Malayalam, Dodda  chirayuta in Kannada and ceti in Tamil.

Basella (Basella alba syn Basella rubra) is also known as Poi, Ceylon spinach, Malabar spinach, Indian spinach or vine spinach. It is a popular leaf vegetable grown in almost all parts of India and widely grown in North-East and South India. Besides India, it is widely cultivated in tropical Asia and Africa. It is a climbing perennial plant, but is sometimes cultivated as an annual vegetable. The plant is succulent with tender leaves. It is a fast growing, productive leaf vegetable for home gardens and market growers. 

Composition
Young shoots and leaves of Basella are a rich source of calcium, iron, and Vitamins A and C.

Uses
Fresh tender leaves and stems are consumed as leaf vegetable after cooking. It is preferable to cook the leaves in soups and stews as half of soluble solids may be lost by boiling in water. Red cultivars contain a colouring matter in leaves, stems and ripe fruits reported as a dye for colouring food. Because of its mucilaginous nature of leaves and stems, it is used as poultice. Juice of leaves is prescribed against constipation, especially for children and pregnant women (Burkill, 1935).

Syn.cordifolia Cogn.,C.indica Wight and Arn,Bryonia grandisL. Family.Cucurbitaceae) is an aggressively climbing vine that  spreads quickly over trees, shrubs, fences or other supports. It is also called  scarlet fruited gourd or little gourd. The name is derived from the Latin coccineus, meaning scarlet,in reference to fruit colour (Wagner et al.,1999). Vernacular names are kundru (hindi), kovakka, kova (malayalam), kovakkai, kovai(tamil), thondakayi (kannada), dondakaya (telugu). Coccinia shoot is a good source of calcium, iron, and fiber and has good texture (Anon., 2002). It is grown mostly in homestead farms for tender fruits in almost all regions of the country. It bears fruits almost through out the year where mild winter prevails. In regions where severe winter occurs, it bears fruit for 8-9 months. Although this is considered as a an under utilized vegetable, it is grown extensively in Chattisgarh, West Bengal, Bihar and Karnataka on a commercial scale and giving remunerative returns to  farmers. Availability of better cultivars and knowledge of  many other preparations and uses of this crop can propel its evolution from an under utilized species to an important horticultural crop in Indian Agriculture.

Spine gourd or teasle gourd (Momordica dioica : Roxb Syn.Momordica roxburghii G. Don, M. wallichi, M J Roem) is a rhizomatous, perennial and distinctly dioecious species of family Cucurbitaceae and  grows widely in many parts of India, mostly confined to  Eastern and South Eastern states. Vernacular names are kartoli,kakrol,meethakarela, padora, bhatt karela, palupagal, tumba, tumpai, golkandra, kankad, kankera, bara-karela, kankoda, kankodan, kantola and phalguni. In India it is mainly grown in Orissa, Bihar, and West Bengal .It is  found in nature in the Punjab, Uttar Pradesh, Rajasthan, Madhya Pradesh, Kerala and Maharashtra especially in areas with the least human interference. This is  a delicacy for its typical taste, attributed to high seed protein content. It also contains high amount of carotene (162 µg/100 g of edible portion) (Gopalan et al., 1982) and its tuberous roots have medicinal value too. It is a cheap source of vitamins and minerals (Bhuiya et al., 1977). This vegetable is gaining popularity  because of its high demand in  market. Certain limitations like lack of awareness among  farmers, non-availability of quality planting materials of standard varieties, unpredictable sex ratio in seedling progenies are arresting the expected spread of this nutritious vegetable (Ali et al., 1991).

Sweet gourd, (Momordica cochinchinensis) (Cucurbitaceae),  called as ‘spiny gourd’,  ‘crow cucumber’, ‘spike fruited crow cucumber’ or ‘elephant bitter gourd’ and ‘gac fruit’ is a wild vegetable, native to East and South East Asia comprising India, Myanmar, Philippines, Vietnam, Thailand, Japan, China, Indonesia and New Guinea (de Wilde and Duyfjes, 2002). It is by far the most extensively distributed and more variable species among the genus Momordica L. Even though, it is restricted to Andaman Islands and Assam in India, it occurs as a natural component of coastal scrub jungles and partially opened forests especially stream banks in South, Middle and North Andaman Islands (Joseph 2005). It is cultivated in Bihar, Jharkhand, Orissa, West Bengal and Assam by tribal people in their homegardens. It is grown as a vegetable in China and Japan (Jeffrey 2001). It is known by various vernacular names as ‘Adavi kakrol’ (Telugu), ‘Hathiya kankad’, ‘Gol kakra’ (Bengali) and ‘Jangli Kakrol’ (Andaman Islands).

Teasle gourd, Momordica subangulata Blume ssp. renigera (G. Don) de Wilde (Cucurbitaceae), is a semidomesticate or a wild vegetable native to Assam-Myanmar region. As in  other dioecious Momordica species, it’s taxonomy and nomenclature were confused with M. dioica Roxb. (spine gourd=kartoli) and M. cochinchinensis (Lour.) Spreng. (sweet gourd=kakrol) (Joseph et al.2006). It is known by many vernacular names like kaksa (Bihar), lamkarote (Manipur), meeta chotela (Sikkim), kakrol (Bengal, Tripura), kakrul (Mizoram), bhat karela (Assam) and gantola (Bangalore). Allied to M. dioica, M. cochinchinensis and M. sahyadrica Joseph and Antony, in general morphology it is distinct from these species in its branched tuberous roots, cordate dentate unlobed leaves, prominently networked leaf veins, large creamy flowers with bull’s eye mark in three inner petals (also in M. cochinchinensis), reniform male bracts, very long female flower stalks and medium sized softly echinate dome shaped fruits with remnant ridges (Fig.1). A few workers reported it as tetraploid form of M. dioica (Misra and Sahoo 1983). It is referred to as M. cochinchinensis by Patnaik and Patnaik (1976), Shadeque and Baruah (1984) and Ram et al. (2002) and M. dioica by Maurya (1976) and Ali et al. (1991), whereas a recent revision of the genus in Malaysia and SE Asia by De Wilde and Duyfjes (2002) proved its botanical identity as Momordica subangulata ssp. renigera  (G. Don) de Wilde.

Strobilanthes (of Acanthaceae) is a morphologically diverse and species rich genus extensively distributed in South and Southeast Asia (Carine,1997 and Moylan et al 2004). Approximately 74 species are found along the Western Ghats of Southern India - Sri Lanka biodiversity hotspot (Carine, 1997). The mass flowering of Strobilanthes with blue coloured flower makes them promising plants for gardens.  The genus is subjected to several systematic revisions recently (Bennett and Scotland 2003, Carine et al. 2000, 2004 Carine and Scotland 1998, 2000, 2002 and Venu 2007). The most distinguishing character of the genera (Strobilanthes) is its monocarpic nature, i.e., flowers after a long period of vegetative growth ranging 1-15 years and in a few cases flowers after 35 years. Bremekamp (1944) used the term ‘plietesials’ for such monocarpic plants that take several years to reach maturity before flowering.

White yams are food plants belonging to genus Dioscorea and family Dioscoreaceae with about 600 species. White yams are among the 10 species of edible yam and belong to species rotundata (D. rotundata Poir). It originated in West Africa.

White yams are herbaceous annual climbers. Its vine arises from large underground stem that is technically called tuber rather than root (Armstrong, 1996). The underground stem thickens to produce one or more tubers which vary in size and shape. The plant bears green flowers that turn brown on maturity and when dry. The aerial stem may be smooth or thorny which could be round/cylindrical (O’ Hair, 1984). Vines can grow to sizeable lengths if provided with a rigid support. Vine length varies within and among species. The leaves could be alternate or opposite, usually heart shaped and may be smooth or hairy. In some varieties, bulbils are formed but this is real in white yam.

Introduction

Biological-based crop protection against plant viruses aims to develop sustainable, non-chemical, crop management technologies that utilize natural-occurrants and phytotoxins to control economically important diseases. In this area the efforts were made since the realization that viruses can not be controlled directly unlike other pathogen.

At present in the absence of any direct control of the viruses, strategies so far have been adopted to control them indirectly; like controlling the vectors of viruses (insects, nematodes, fungi etc.), using barrier crops, and use of resistant cultivars. However, success had been very limited. As the biological sciences advanced, many biological systems were utilized to control the diseases in plants caused by viruses, promising among them is using apical-meristem culture techniques to produce virus free plantlets as starting material.

‘To a people famishing and idle, the only acceptable form in which God can dare appear is work and promise of food as wages’ — these were the words of Mahatma Gandhi when he was healing the wounds arising from the Hindu-Muslim divide at Naokhali in 1946. He thus stressed the symbiotic bonds among work, income and food security.
 
Eradication of hunger and poverty is also the first among the UN Millennium Development Goals, which in my view represent a Global Common Minimum Programme for human security and well-being.
 
Achieving this goal is fundamental for achieving the other goals relating to education, gender equality, child mortality, control of HIV/ AIDS, malaria and other diseases and, above all, environmental sustainability. Unfortunately, progress in the reduction of hunger and poverty is poor in most developing countries excepting a few like China. FAO estimates that as a result of the rise in the prices of basic staples, about 75 million more people have been added to the list of those going to bed hungry during 2007. As a single nation, we in India have the largest number of malnourished persons in the world.

Mahatma Gandhi mentioned to those who wanted to serve rural India that their most useful contribution would be in linking brain with brawn.  Only such a combination of intellect and labour can lead to sustainable advances in the productivity and profitability of agricultural holdings.  West Bengal’s Bidhan Chandra Krishi Vishwavidyalaya (BCKV), almost four decades old, is a fitting example of such work.  It has helped the development of two more universities in the State, namely, West Bengal University of Animal & Fishery Sciences and the Uttar Banga Krishi Viswavidyalaya.  It is important that these three universities function like a consortium of educational institutions mandated to help in achieving agricultural advance, agrarian prosperity and rural transformation in the State.

Livestock and livelihoods are intimately related in our country.  The ownership of livestock is more egalitarian than that of land.  Similarly, West Bengal is full of fish ponds.  It is important that cultivation of crops like rice is done by adopting integrated pest management procedures, so that the water in which fish is cultivated does not get polluted with pesticides.

Genus : Mangifera 
Species : indica L.
Family : Anacardiaceae
Chromosome No. : 2n = 40

In the early Sanskrit literature  Amra, mango was known to Indian at very early times. Mango occupies an important place in the Hindu mythology as well as in various religious observances. Various archaeological discoveries also mention the presence of Mango in India. Alexander the Great in 327 BC during his invasion found mango cultivation in the Indus Valley. The Buddhist and Chinese pilgrims in their travel notes have also indicated mango cultivation. Amir Khusru a famous Turkoman poet has quoted ‘Mango as the pride of garden, the choicest fruit of Hindustan’. During the Mogul Emperors, mango occupied an important place in fruit culture. The Ain-e-Akbari an encyclopaedia work written during the rule of Akbar, gives detail account of mango, varietal characteristics, quality etc. In praise of mango, British authors lavished huge praise and designated it as King of Fruits and that the Goa mango is perhaps the largest and most delicious, wholesome and best tasted of any fruit

Genus : Ananas 
Species : comosus L. Merr
Family : Bromeliaceae
Chromosome No. : 2n = 50, 75, 100

Pineapple (Ananas comosus L) ‘Golden Queen’ belongs to order Farinosae and family Bromeliaceae. The name is derived from genus Bromelia in honour of Olaus Bromel, a Swedish botanist. Herbs or sub-shrubs  mostly epiphytic, has the distinction to be one of the major food plants of the world, which was selected, domesticated and developed by the early pre-historic people, who passed this valuable knowledge gathered for hundreds of years through earlier civilization. Pineapple developed to its present form in its natural habitat – New World, and was not know to the Old world before Columbus discovered America. Red Indians the local inhabitants of South America at that early time had already developed and named number of varieties which were partially selected by them or their ancestors based upon various desirable horticultural traits like fruit size and quality, earliness, long storage, and absence of seeds etc.

Genus : Phoenix 
Species : dactylifera L.
Family : Palmae (Sub family-Phoenicoideae)
Chromosome No. : 2n=36

Date (Phoenix dactylifera) Finger bearing is derived from Greek word Phoenix. This ancient plant has been named phoenix after the mythological bird which was native of Egypt and also very likely after ‘Phoenicia’, an old country on the coastal regions of Syria where palms were found growing in plenty. The date palms are usually planted as ornamental for their luxurious crown which is spreading at the top. The trunk and crown is covered with stiff pinnate leaves, suckers or offshoots at the base, markedly transforms the site or scene and provide an exotic charm.

Genus : Annona 
Species : cherimola Mill.
Chromosome No. : 2n = 14, 16

Other species
A. squamosa 2n =14, 16 L.
A. reticulata 2n =14, 16 L.
A. muricata 2n =14, 16 L.

Collectively all the Annona fruits technically are syncarpous is composed of more or less numerous carpels coherent with the fleshy receptacle. Fruit has been described as ‘Deliciousness itself’ compared to pineapple, mangostean and cherimoya the finest fruits of the world. Its native place is the mountains of Ecuador and Peru as terra-cotta models of cherimoya fruits are frequently obtained from prehistoric graves in Peru. The word cherimoya is from the Peruvian name ‘cherimuya’ meaning cold seed. Although originated close to the equator the fruit attains best quality or performs better where climate is cold and relatively

Genus : Ficus 
Species : carica L.
Family : Moraceae
Chromosome No. : 2n = 26

Fig along with fruits like date palm, grape (vinifera) and olive was regarded as an important food crop of the ancient civilization that developed in the Mediterranean region. The fig description occurred prominently in number of songs as well as in the mythological and historical events that took place at that time. Fig is thought to be native of southern parts of Arabian peninsula, Italy, Balkan and USSR region. Fig was in cultivation as early as 300 BC and from Arabian peninsula it spread to Turkey, Syria, Iraq and later to many subtropical parts of the western hemisphere.

Genus : Artocarpus 
Species : heterophyllus L. (Lamk.)
Family : Moraceae
Chromosome No. : 2n = 56

Afamous traveller John de Marignolli has described jackfruit as ‘there is again another wonderful tree, which produced fruit on the trunk, and was very pleasing to see and the fruit was as big as a lamb‘. The rind is hard and has to be cut open by a sharp knife, the pulp inside is of pleasant flavour sweetness of which matches the honey and usually contains 500 or more chestnut like seeds which are eaten after roasting. In its place of origin (India) where it has been grown since ancient times it is held in great esteem. Theopharatus and Pliny who wrote about jackfruit also expressed similar opinion about the fruit. In India several vernacular names are given like Kanthal, Kathal, Panasa and Kantaka.

Genus : Zizyphus /Rhamus
Species : mauritiana Lam. jujube Lam.
Family : Rhamnaceae
Chromosome No. : 2n = 24, 48, 96

According to famous writer David Fairchild it is the jujube, the large fruited Chinese type which is at its best compared to the inferior type which are in abundance in Arabia, northern India and southern Europe. The resultant inferior fruits are either from a different species compared to those of China or from varieties which have not been much influenced and improved by selection and cultivation.

Genus : Fragaria
Species : ananassa Duch.
Family : Rosaceae 
Chromosome No. : 2n=14, 25, 42, 56

Strawberry is relatively small genus comprising of usually low perennial creeping herbs, wild forms found wide spread in temperate and subtropical parts of the world. Cultivated strawberry is highly polymorphic and wide range of diversity present in the wild germplasm has resulted in the development of present day strawberry. Today it is grown throughout the world and is rated high in the list of liked foods. The breeders have contributed enormously towards the development of cultivars with wide adaptability and through the incorporation of desired traits helped to improve its quality dramatically over its wild forms. Today strawberry is known for its distinct delicate flavour, attractive fruit shape and colour and has made a special niche in the soft fruits culture. The cultivated strawberry Fragaria x ananassa Duch has resulted from the crossing of two local America species F. chiloensis (L) Duch. and F.virgianana, Duch.

Genus   : Rubus
Species : idaelus L. (European Red
                Raspberry) strigorus Michx. 
                (Red Raspberry occidentalis L. 
                (Black Raspberry)
Family : Rosaceae
Chromosome No. : 2n = 14 
                            (variable 28, 42, 56, 84)

The blackberries, raspberries, dewberries, boysenberry are collectively referred to as bramble fruits.  Mostly found in the north hemisphere. They belong to the family Rosaceae and genus Rubus. The family has nearly 250 species besides also has large number of apomictic segregates. Usually shrub, the woody species has biennial canes i.e. in the first year remains vegetative and in the second year it fruits and dies. Leaves variable in form from simple leaf to pinnately or palmately compound.  Flowers usually occur in clusters and are variable in colour, white pink to red.  Fruit is an aggregate of number of small drupes.

Genus : Prunus / Amygdalus
Species :  dulcis L. communis, Fritsch.
Family : Rosaceae
Chromosome No. : 2n = 28

Almond tree amongst the deciduous temperate fruits is the first crop to bloom in spring as it responds readily to warm temperature and also for its relatively low chilling requirements. The almond is thought to be one of the oldest nut crop used by man since very long.  However, its specific climatic requirements have greatly restricted its commercial production to limited regions of the world.  The almond blossoms are large attractive pink coloured very sensitive to spring frost, but at the same time the nut require high heat quantum at maturity, cool rainy weather during flowering restricts cross pollination and induces fungal infections. In the mild winter regions almond trees are limited by the fact that distinct chilling requirement for growth and flowering are not met with.

Genus : Malus  
Species : domestica (Borkh.)
Family : Rosaceae 
Chromosome No. : 2n = 34, 51, 68

Like many other fruit crops, apple too is a very old crop in cultivation. Theophratus as early as third century B.C. has indicated that the Greeks and Romans knew apple culture and among the temperate fruit crops, apple is the most ubiquitous of all fruits and is extensively grown all over the world. Huge genetic variability present has permitted the better adapted types to be selected for plantation under different agro climatic conditions.  Through continuous selection both from wild population and also from hybrid seedlings, varied types or varieties have been selected, some are suitable for cultivation in warm temperate regions and others for extreme cold conditions.  This has resulted/added to the gene pool of desired types of apples enormously. At present cultivars are available destined for specific purposes like dessert, cooking, juice, cider and for large number of other uses.

Genus : Pyrus 
Species : communis L.
Family : Rosaceae 
Chromosome No. : 2n= 34

Pear like apple is also a prehistoric crop and unlike apple its cultivation in Europe started much before that of apple at an early time. According to Homer (1000 B.C.) the ancient Greek said that pears were one of the “gifts of god’s”. Romans also enormously added to the knowledge of pear cultivation. Pliny in Rome had described 35 cultivars (23-79 AD). The variation in fruit characters described earlier match to the range of fruit characters which are found in presently grown commercial cultivars. In Europe for its buttery soft texture and distinct aroma many consider European pear as the most detectable of all temperate fruits, while the Asian pears for their firm crisp texture and juiciness are preferred more in China and Japan – their native place. Pears are readily consumed as fresh, dried, cooked or as preserves or to make perry and pear wine.

1. Introduction

Medicinal plants are rich source of novel drugs that forms the ingredients in traditional systems of medicine, modern medicines, nutraceuticals, food supplements, folk medicines, pharmaceutical intermediates, bioactive principles and lead compounds in synthetic drugs (Ncube, 2008).  In recent years, antibiotic resistance has become a global concern. This drives the need to screen medicinal plants for novel bioactive compounds as they are biodegradable, safe and have fewer side effects (Prusti et al., 2008). Higher plants, as a source for new potential drugs is still largely unexplored and screening of active agents helps to design the lead molecule later in drug development.

Aegle marmelos(L.) Corr., belongs to the family Rutaceae, and is popularly known as Vilvam tree (Tamil) (Gamble, 1935; Mathew, 1983). It is an indigenous tree found in Iran, India, Myanmar, Pakistan, Bangladesh and most of Southeast Asian countries (Singh, 2009).  A. marmelos has been used from time immemorial in traditional systems of medicine for relieving diarrhoea, dysentery, stomachic, digestive, astringent, peptic ulcer and respiratory infection (Nadkarni, 2000). The fruit, leaves, bark and root are edible and possess medicinal values. The unripe dried fruit used to be astringent, digestive and stomachic, the ripe fruit is aromatic, cooling and is used as laxative.

1. Introduction

India has a rich tradition of plant-based knowledge on healthcare. It is reported that traditional healers use 2500 plant species and 100 species of plants serve as regular sources of medicine (Pei, 2001). Traditional medical knowledge of medicinal plants and their use by indigenous cultures are not only useful for conservation of cultural traditions and biodiversity but also for community healthcare and drug development in the present and future. Medicinal plants have curative properties due to the presence of various complex chemical substances of different composition which are formed as secondary plant metabolites in various parts of the plants such as roots, shrubs, leaves, flowers etc. (Gurib-Fakim, 2006).

A large number of plants are equally used by tribals and folklore traditions in India for treatment of cuts, wounds and burns (Biswas and Mukherjee, 2003). A wound is a type of injury in which skin is torn, cut or punctured, that disturbance in the normal skin anatomy and function. Wound healing consists of orderly progression of series of events that establish the integrity of damaged tissue and thereby the affected parts repair itself after injury. The wound healing herbs encourage blood clotting and induce the healing of wounds (Sudhakar et al., 2003; Nagori and Solanki, 2011; Binu Thomas et al., 2012).

1. Introduction

Phytochemicals are biologically active compounds found in plants, which act as a resistance system for host plants and protect them from biotic and abiotic stresses. Phytochemicals act as antioxidants, which react with the free radicals present in the cell. Free radicals are formed in the cell as a result of aerobic metabolism and these radicals are biologically active that damages the macromolecules like DNA, proteins and lipids resulting in oxidizing chain reactions (Moussa et al., 2011). Oxidative stress damage leads to various disorders like autoimmune disorders, cancer, rheumatoid arthritis, cataract, aging, cardiovascular diseases and other neurodegenerative disorders (Uttara et al., 2009). The major actions of antioxidant in the cells are to protect them against the damage caused by reactive oxygen species (ROS).

Medicinal plants are good sources of antioxidants such as vitamin E, ascorbic acid and carotenoids. There are huge numbers of medicinal plants that are veiled in the environment. In ancient times, different plant species have been used for the treatment of human ailments from fever to cancer (Devendrakumar et al., 2009).

COMMUNITY DEVELOPMENT (CD)

Meaning of Community Development

Community development is an exclusive term. It is used frequently to encompash every efforts towards the advance of community interest.

Community

A community is a group of people who live in a particular geographical area and have an interest on each other and have a purpose of making a living.

Development

It means growth or maturation. It is gradual and sequencial phases of changes. Example: Life from child to death

Definition of Community Development

1. It is bringing forth the potential abilities and qualities of a group of people who live together in a common territory or region and who have an interdependent relationship with each other.

2. Community Development is a continuous process of social action by which the people of community do the following things.

(a)   Define their common and group needs.

(b)   Organize themselves formally and informally for democratic planning and action.

(c)   Make group and individual plans to meet their need and solve their problems.

(d)   Execute their plans with maximum reliance upon their own resources.

(e)   Supplement their resources from outside community when necessary with services and materials.

3. Carl Taylor’s Definition

Community Development is a method by which people in villages are involved in helping to improve their own economy through their own effort. Thereby, they are effective working groups in the programme or national development.

Materials Required

Fruits/ vegetables, washing tanks/ containers, size grader, fruit waxes, packaging material, neem based fungicides etc. 

Procedure

    1.    Take available fruits or vegetables 
    2.    Wash them in plain water or chemical solution as told by the teacher
    3.    Shade dry the fruits / vegetables
    4.    Prepare suitable wax solution
    5.    Apply wax on to the fruits as told
    6.    Record the fruit weight before packing
    7.    Pack the fruits properly in the trays / boxes depending upon the type and purpose
    8.    Do curing if required 
    9.    Do pre-packaging, if desired
    10.    Label the package for desired information
    11.    Keep the fruits for storage
    12.    Record the observations pertaining to weight loss and rotting periodically depending upon the commodity and its shelf life.

Materials Required

Tomato fruits, pulper, steel pans, weighing balance, refractometer, sugar, salt, spices, acetic acid, citric acid, muslin cloth etc.

Procedure
I. Puree

    1.    Extract fruit pulp by Hot process as told by the teacher.
    2.    Record the TSS of the pulp.
    3.    Concentrate the pulp by heating to 12º Brix (heavy puree)
    4.    Hot fill the boiling puree into pre-sterilized glass bottles (200 ml cap.)
    5.    Seal the bottles by crown corking
    6.    Heat process in boiling water in a big aluminium pan for 15-20 min. 
    7.    Cool the bottles to room temperature as quickly as possible.
    8.    Label and store at a dry place.

Chikungunya has been emerged as an important public health problem in the Indian Ocean Islands and India recently. Epidemiological, geographical and demographic data suggested that 1.38 million people were affected by the disease in India in 2006. The actual incidence of the disease may have been higher but underreporting did not reveal the actual picture. A large proportion of those who became ill consulted private health care professional or sought alternative medical care such as Ayurveda or homeopathy not included in the data collected by Govt. officials.

During the epidemic, rural hospitals, private and public clinics flooded with victims of Chikungunya. The virus did not kill its victims but inflicted considerable pain and misery besides local, regional and national financial burden towards health care. Only National Institute of Virology (NIV) and National Institute of Communicable Disease (NICD) provided accurate diagnosis of the disease. The Chikungunya assumed epidemic proportions because viral agent mutated, the vectors discovered the new ways to spread and the host lacked immunity to fight the disease. Modern transport systems - cars, buses, trains and aircraft particularly the movement of humans played a key role in spreading the disease that helped to maintain man-mosquito man cycle.

Etiology: First isolated in Tanzania in 1953, Chikungunya is under genus Alphavirus and family Togaviridae. It contains a single stranded positive sense RNA with a length of 12 Kb coding 4 non-structural (nspl - nsp 4) and 3 structural proteins (E1-E3). Among the structural protein, El protein correlates with serological response in human hosts and also modulates penetration of the virus in the mosquito species. Genetic analyses and historical accounts suggest that the Chikungunya virus originated in tropical Africa and subsequently evolved into 3 distinct genotypes - the East African, the West African and the Asian genotypes. The Asian genotypes have a high degree of nucleic acid sequence homology among themselves but the African strains exhibit wider sequence diversity and have been shown to undergo genetic micro-evolutions even during the course of an epidemic. Prior to the 2006 epidemic in India, the 3 genotypes were restricted to the geographical areas only. Previous outbreaks in India (1963 and 1973) were caused by the Asian genotypes, but the 2005 epidemic in the Indian Ocean Islands and the 2006 epidemic in India have been attributed to the East African genotype which arrived in India 5 years ago.

This is an acute viral encephalitis having public health importance. It is under the genus Flavivirus and family Flaviviridae. The genus Flavivirus comprises of group of arbovirus though a few members of the genus have no known vector. The genus contains over 70 viruses of which 40 are mosquito borne, 16 are tick borne and 18 have no known vector. The type species of the genus is yellow fever virus. Although all flaviviruses are serologically relate, they can also be grouped serologically into distinct groups: dengue serological group, the JE serological group and a less serologically cohesive yellow fever virus group. The disease has been recognized in Japan since 1871 and was named Japanese B encephalitis to distinguish it from 'encephalitis A' (encephalitis lethargica, Von economo's disease) which was then prevalent. The virus was first isolated in Japan during an epidemic in 1935.

Etiology: JE virus is under the genus Flavivirus and family Flaviviridae. The genome is about 11 kb which encodes 3 structural proteins (C, capsid protein, PrM the membrane precursor protein that is cleaved proteolytically by a cellular protease to form the M protein in mature virions, and E, envelope protein) and 7 non-structural (NS) protein (NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5). The Eglycoprotein is the most immunologically important protein. The Japanese encephalitis serological group of flaviviruses comprises eight virus species and two subtype viruses with a geographic range encompassing all continents except Antarctica. The major virus species and their geographic range are as follows: the Japanese encephalitis virus ( JEV) in eastern, southern and southeastern Asia, Papua New Guinea and the Torres Strait of northern Australia; the West Nile virus (WNV) in Africa, southern and central Europe, India, the Middle East and North America and as Kunjin virus ( a subtype of WNV) in Australia and Papua New Guinea; the Murray Valley encephalitis virus (MVEV) in Australia, Papua New Guinea and the western Indonesian archipelago and the St. Louis encephalitis virus (SLEV) in North and South America. The other minor members of the group are Usutu (USUV), Koutango and Yaounde viruses in Africa; Cacipacore virus in South America and Alfuy, a subtype of MVEV, in Australia.

Marburg virus (MV) disease was named after the West German town where the largest outbreaks of human infection was reported. It is also known as Vervet monkey or green monkey disease as monkey species introduced the disease in Europe. Marburg disease virus was reported in 1967 in Frankfurt-on-Main and Marburg, West Germany and Belgrade, Serbia in vaccine factory workers who came in contact with blood, organs or cell cultures of African green monkeys. Ebola and Marburg hemorrhagic fevers are severe zoonotic diseases seen not only in humans but also in non-human primates. Most species of Ebolavirus and the only known species of Marburg virus occur in Africa. Primates are infected sporadically from an unknown source probably bats. Humans seem to become infected directly from bats in caves, as well as when they, come in contact with tissues from infected apes and other species. Once the virus has entered the population, it can spread from person to person. Some epidemics affect hundreds of people. Although the mortality rate varies, 90% case fatalities have been reported in the most pathogenic viruses. No vaccine is available and the only treatment is supportive. Epizootics in gorillas and chimpanzees are equally serious, and appear to threaten the survival of these species in the wild. Other wild mammals including duikers also seem to be killed during outbreaks. One species of Ebolavirus, Reston Ebolavirus, occurs in the Philippines. Until 2008, this virus was known only as an infection of nonhuman primates. It does not appear to be a human pathogen; although some people in contact with this virus have seroconverted, they remained asymptomatic. In December 2008, Reston ebolavirus was discovered in pigs during an unusually severe outbreak of porcine reproductive and respiratory syndrome (PRRS) in the Philippines.

Etiology: Ebola and Marburg hemorrhagic fever are caused by members of the genera Ebolavirus and Marburgvirus, respectively. These viruses are the only members of the family Filoviridae. The genus Ebolavirus contains four recognized species: Zaire ebolavirus (formerly Zaire Ebola virus), Sudan ebolavirus (formerly Sudan Ebola virus), Ivory Coast ebolavirus (formerly Cote d'Ivoire Ebola virus) and Reston ebolavirus (formerly Reston Ebola virus). At least 13 strains of these viruses have been identified. A fifth species, tentatively named Bundibugyo ebolavirus, was isolated from a recent outbreak in Uganda. Marburg virus contains a single species, Lake Victoria Marburgvirus (formerly Marburg virus). Six strains of Lake Victoria Marburgvirus had been recognized as of 1990; at least nine genetically distinct strains were identified from a more recent outbreak in the Democratic Republic of the Congo. Marburg virus contains ssRNA as genome. The virion is highly pleomorphic and measures 80 nm in diameter and 700-1000 nm in length. Filoviruses can be destroyed by autoclaving. Treatment with sodium hypochlorite (1:100 dilution of household bleach) or standard hospital disinfectants can also be used. Ebolaviruses are reported to be susceptible to 2% sodium hypochlorite, 2% glutaraldehyde, 5% peracetic acid and 1% formalin. These viruses can also be inactivated by ultraviolet light, gamma irradiation, 0.3% betapropiolactone for 30 minutes at 37°C (98.6°F), or heating to 60°C (140°F) for 1 hour. Marburgvirus is susceptible to 1% sodium hypochlorite, 2% glutaraldehyde or formaldehyde, ultraviolet light and heat.

Synonyms: Splenic fever, wool sorter's disease, Tarka

It is an infectious disease of livestock and worldwide in distribution. This is a zoonotic disease. It is also known as splenic fever because of enlargement of the spleen (splenomegaly).

Etiology: It is caused by a rod shaped bacteria of 4-8 µm X 1-1.2 µm known as Bacillus anthracis. It is non-motile, capsulated, aerobic, spore bearing and Gm+ in nature. The organisms remain in chains. It was the first pathogenic bacterium to be observed under the microscope, the first communicable disease to be transmitted by inoculation of infected blood, the first bacillus to be isolated in pure culture and shown to possess spores and the first bacterium used for preparation of an attenuated vaccine.

The capsule is polypeptide in nature being composed of a polymer of d(-) glutamic acid. Capsules are not formed under ordinary conditions of culture but only if the media contain added bicarbonate or are incubated under 10-25% CO₂. Capsules are formed in the body of an infected animal.

Spores are formed in culture or in the soil, but never in the animal body during life. Spores are formed only in the presence of O₂. Spores are central, elliptical or oval in shape. The spores are very resistant to physical and chemical agents. They have been isolated from naturally infected soil for as long as 60 years. They resist dry heat at 140°C for 1-3 h and boiling for 10 min.

Anthrax bacilli is Gm + and non-acid fast. When blood films containing anthrax bacilli are stained with polychrome methylene blue for a few seconds and examined under the microscope, an amorphous purplish material (capsule) is noticed around the bacilli called M'Fadyean's reaction. On agar places, irregularly round colonies are formed 2-3 mm in diameter raised, dull opaque, grayish white with a frosted glass appearance. Under the low power microscope, the edge of the colony is composed of long, interlacing chain of bacilli, resembling locks of matted hair. This is called "Medusa head appearance." Virulent capsulated strains form rough colonies while avirulent or attenuated strains form smooth colonies. On gelatin stab culture, a characteristic 'inverted fir tree' appearance is seen with slow liquefaction commencing from the top.

Salmonellosis is one of the important zoonoses, acute or chronic in nature, affected by all species of animals, birds and humans and characterized by gastroenteritis.

Etiology: The genus Salmonella belongs to the family Enterobacteriaceae. Currently there are 2463 serotypes (serovars) of Salmonella. According to the current system used by the CDC the genus Salmonella contains two species each of which contains multiple serotypes. The two species are S. enterica, the type species and S. bongori. The S. enterica subsp. enterica is divided into 6 subspecies. S. enterica subsp. enterica I is divided into serotypes, for example serotypes enteriditis, typhimurium, typhi and choleraesuis. The majority of 2463 Salmonella serotypes belong to S. enterica subsp. I and associated mainly with warm blooded vertebrates and are responsible for most Salmonella infections in humans and domesticated animals. Other serovars are restricted in their host range causing severe systemic disease in only one host. For example, S. typhi is restricted to infection in humans, S. abortusovis to infection in sheep, S. dublin in cattle, S. abortusequi in horses and S. choleraesuis in pigs. The serotypes (serovars) that most commonly cause Salmonellosis in farm animal species are as follows:

Cattle: S. typhimurium, S. dublin, S. newport

Sheep and goat: S. abortusovis, S. typhimurium, S. dublin, S. anaturn

Pig: S. typhimurium, S. choleraesuis

Horses: S. typhimurium, S. abortusequi, S. anatum, S. newport, S. enteritidis, S. arizona, S. angona, S. heidelbers

The organisms are Gm- and rod shaped of 1-31μX0.5-0.8μ in size. They are motile with peritrichous flagella except one type S. gallinarum pullorum which are always non-motile. They do not form capsules or spores but may possess fimbriae. Salmonellae are aerobic and facultatively anaerobic growing readily on simple media over a range of pH 6-8 and temperature 15-41°C (optimum 37°C). Colonies are large, 2-3 mm in diameter, circular and smooth. On MacConkey and deoxycholate citrate media colonies are colorless due to absence of lactose fermentation. On Wilson and Blair bismuth sulphite medium, jet black colonies with a metallic sheen are formed due to production of H2S. S. paratyphi A and other species do not form H2S, produce green colonies. Selenite F and tetrathionate broth are commonly employed as enrichment media.

It is a non-contagious, nonfebrile infectious disease of mammals caused by toxin of Cl. tetani and characterized by sposmodic contraction of skeletal muscles.

Etiology: It is caused by Cl. tetani. It is a Gm⁺, slender bacillus, 4-8μ X0.5μ in size. It has a straight axis, parallel sides and rounded ends. It occurs singly and occasionally in chains. The spores are spherical, terminal and bulging, giving the bacillus the characteristic 'drum stick' appearance. It is non-capsulated and motile by peritrichate flagella. Young cultures are strongly Gm+ but older ones may be Gm-. Cl. tetani is widely distributed in soil and in the intestine of man and animals. Soil may have good concentration O organisms if it is fertilized with animal manure. Some strains have been identified but they have same antigenic type of toxin and can be neutralized by single toxin. There are two types of antigen H (flagella) and O (somatic). The H antigen is thermolabile and O antigen is thermostable and responsible for spasms.

It is an obligatory anaerobe that grows only in the absence of O₂ It grows on ordinary media. Growth is improved by blood and serum but not by glucose. The spores are very much resistant and can persist in the soils for even years. The spores can be destroyed by boiling at 115°C for 30-60 min.

The organisms produce 3 types of toxins. The toxins are tetanospasmin (neurotoxin), tetanolysin (haemolysin) and fibrinolysin. Tetanolysin is a heat labile, O₂ labile haemolysin antigenically related to the O₂ labile haemolysin produced by Cl. welchii. This lysin is active against RBCs of several animal species. Tetanospasmin is responsible for clinical manifestation of tetanus. It is O₂ stable but relatively heat labile. It gets toxoided spontaneously or in presence of low concentration of HCHO. It is a good antigen and is specifically neutralized by the antitoxin. It exists in two forms. A monomer of MW 68,000 which is toxic and a dimer which is non-toxic but antigenic. The purified toxin is active in extremely small amounts and has an MLD for mice of about 50-75X10^-6 mg. Horse is the most susceptible animal. Guinea pig, mice, goats and rabbits are susceptible in that descending order. Birds and reptiles are highly resistant. Frogs which are insusceptible, may be rendered susceptible by elevating their body temperature.

Q fever (query fever) is an important global occupational zoonotic disease caused by a bacteria Coxiella burnetti. It is an OIE notifiable disease and showing an emerging and reemerging trend in different parts of the world. It is having great public health importance as the pathogen is transmitted through almost all routes but predominantly by aerosols from reproductive discharges of animals of farm and pet animals which serve as main reservoir of infection. The organism is shed in milk and foods of animal origin for very long period. Q fever is associated with a wide clinical spectrum and most of its acute infections are either asymptomatic, mild or resemble to influenza. Presently, the infection is endemic in many areas and its prevalence has been confirmed in at least 51 countries including India.

Etiology: C. burnetti was classified as a member of Rickettsiaceae family however, recent scientific investigations on 16S RNA based sequence analysis have shown that it is genetically more comparable to bacterial families covering genus Francisella and Legionella therefore it has been reclassified in the family Coxiellaceae or order Legionellales. It is a Gm-, pleomorphic rods (0.2-0.41μX0.4-1.0μ) or sphere (0.3μ-0.4μ in diameter in size) and an obligate I/C organism. It is relatively resistant to physical and chemical agents. The bacterium occurs in two antigenic forms - the pathogenic form (phase I) found in infected animals or humans and the avirulent form (phase II) obtained by repeated passagein embryonated eggs or in cell culture. Phase II cells are autoagglutinable and are readily phagocytosed in the absence of specific antibodies. Phase I activity is attributed to surface carbohydrate antigen which is soluble in trichloracetic acid and is destroyed by periodate. Phase I is a more powerful immunogen than phase II and elicits antibodies in high titer to both antigens. Phase II antigen gives better results in CFT with human and animal sera. Q fever sera do not react with other rickettsial antigens or with Proteus. In nature, bacterium exists mainly in 3 forms, namely - the small cell variant (SCV), the small dense cells (SDC) which are spore like forms that remain very stable in the environment as extracellular infections particles and the large cell variant (LCV) which is the growing and metabolically active form inside the host. In view of the potential for transmission of this infection from animal to people, the disease has been classified as a notifiable OIE disease.