eChapters

Introduction

Statistics generally refers to information about an activity or a process that is expressed in quantitate as well as qualitative traits. The quantitative research process is well structured whereas the qualitative one is fairly unstructured. It depends upon how a piece of information have been collected and analyzed. A good researchers need to have both types of skill. This article highlights about the operational procedure, about methods, procedures and techniques that are used in analysis of climate related data.

Parameter vs Statistic

When a data is collected with survey of all the individuals (like in a census) and analysed for certain values, it is called a parameter. But when, we guess the same value with a random sample and interpret for whole population it is called a statistic. Environmental parameters are: mean, variance, correlation, regression, heritability, repeatability etc. Again, there are two types of variables: quantitative and qualitative. Quantitative variables can take any value in a specified range. So, they are of continuous type. But qualitative variables are very few types ( 2 ,3 or 4 types) and they are of discrete type. Body weight is an example of quantitative variable whereas, comb type is a qualitative variable.

Good livestock welfare practices demands thorough understanding of the basic nature of the animal. The science of understanding the details of animal behaviour is known as Ethology.  Knowledge about the behaviour of the species being reared proved as an important tool for implementing animal welfare in practical husbandry practices. Now a day’s global community is also advocating for the better livestock welfare standards. Exploiting animal’s natural behaviour could certainly prevent unnecessary sufferings related to handling and restraining during routine farm activities. On the one hand this ensures physical and mental welfare of livestock, while on the other hand improvement in the production is guaranteed. Without involving heavy investment behavioural knowledge based management interventions can bring paradigm shift in welfare status of farm. Therefore, behavioural studies are of great importance in increasing our understanding and appreciation of animals.

Biofertilizers are preparations containing micro-organisms, with capability of mobilizing nutritive elements from non usable form to usable form through biological processes. Bio-fertilizers have definite advantage over chemical fertilizers. Chemical fertilizers supply over nitrogen whereas bio-fertilizers provide in addition to nitrogen, certain growth promoting substances like hormones, vitamins, amino acids, etc, crops have to be provided with chemical fertilizers repeatedly to replenish the loss of nitrogen utilized for crop growth. Bio-fertilizers application in agriculture will have greater impact on organic agriculture and also on the control of environmental pollution, soil health improvement and reduction in input use. Farmers try to increase yields of vegetable crops by mean of heavy nutrition’s. The use of chemical nitrogen and phosphorus fertilizers at high levels had an adverse effect on the accumulation of NH4+, NO3-, NO2- and PO4- in vegetable product tissues. Therefore, clean agriculture recently depends upon using biofertilizers as well as organic in order to produce high yields with the best commodity quality without contamination and less accumulation with heavy metals.

The application of high input technologies such as chemical fertilizers, pesticides, herbicides have improved the production but there is growing concern over the adverse effects of the use of chemicals on soil productivity and environment quality. Thus, integrated nutrient management has become an accepted strategy to bring about improvement in soil fertility and protecting the environment. This strategy utilizes a judicious combination of fertilizers, organic manures and bio-fertilizers. Biofertilizer is a natural product carrying living microorganisms derived from the root or cultivated soil. So they don’t have any ill effect on soil health and environment. Besides their role in atmospheric nitrogen fixation and phosphorous solubilisation, these also help in stimulating the plant growth hormones providing better nutrient uptake and increased tolerance towards drought and moisture stress. A small dose of biofertilizer is sufficient to produce desirable results because each gram of carrier of bio-fertilizers contains at least 10 million viable cells of a specific strain. Biofertilizers means the product containing carrier base (solid or liquid)

Abstract

The study of palm genomics can aid to understand the genetic and molecular knowhow of all biological processes in palms that are vital tothe palm species. This knowledge will allow the path for the fruitful exploitation of palms as biological resources in the development of new cultivars of improved quality and with shortened economic and environmental costs. This knowledge is also vital for the development of new diagnostic tools. Traits considered of primary interest are resistance to pathogens and abiotic stress, fruit quality and yield. Genomics and bioinformatics approaches helped in unraveling the genomes of economicpalsm like coconut, oilpalm and datepalm. The genome characteristics and tools for genome analysis are provided in the chapter. Due to the economic importance of cocoa, many scientific research studies (genome, EST, transcriptome) have been carried out in the past years in this crop also.

Introduction

Plantation crops constitute a large group of crops. The major plantation crops include coconut, arecanut, oil palm, cashew, tea, coffee and rubber; and the minor plantation crops include cocoa (Prapulla and Indira, 2014). India is the third largest producer of coconut and goes in front of the ninety coconut-producing countries of the world (Richard 2013). India is the largest producer and consumer of cashew nuts (Mohod et al., 2011.). India also occupies number one position in arecanut production (Dwivedi et al.,2008) besides being the largest tea producers in the world. While Coffee is the second largest traded commodity in the world, it has, in recent times become an extremely important foreign exchange (Prapulla and Indira, 2014).

Abstract

Biosurfactants (BS) are amphiphilic compounds produced by microorganisms mainly bacteria and are known to contain hydrophobic and hydrophilic moieties that play a major role in reducing surface tension (ST) and interfacial tensions between individual molecules. Chemically, BS is a complex molecules consisting of lipopeptides, glycolipids, polysaccharide protein complex, fatty acids and phospholipids. They are known for their biodegradability, low toxicity, and renewable nature.

Introduction

Under the present scenario of growing human population and shrinking land and water resources for agriculture, expanding abiotic and biotic stresses, increasing genetic erosion; relentless efforts are needed to enhance the plant productivity as well as quality of produce. In fact, improvements in agricultural production as well as food and nutrition conditions depend on land, water and energy resources, which are, undoubtedly, limited and depleting at a rapid pace.

Biotechnological innovations in conjunction with conventional technologies have opened new frontiers in agriculture thereby offering exciting opportunities for increased sustainability and profitability in agriculture. Thus, biotechnological tools complement (rather than replace) the traditional methods used to enhance the agricultural productivity to cater to the needs of third world agriculture (Swarup and Srivastava1990, Rao et al. 1996).

Plant diseases are a threat to world agriculture. Significant yield losses due to the attack of pathogen occur in most of the agricultural and horticultural crop species. More than 70% of all major crop diseases are caused by fungi . Plant diseases are usually handled with applications of chemicals. For some diseases, chemical control is very effective; but it is often non-specific in its effects, killing beneficial organisms as well as pathogens. Chemical control may have undesirable effects on health, safety and cause environmental risks. A promising method for protecting plants against diseases is constructing and employing pathogen-resistant cultivars. Although a number of resistant cultivars have been developed through breeding programs, these cultivars become obsolete in a short time due to the rapid evolution of the phytopathogens and the emergence of virulent forms capable to overcome the plant resistance. Breeders are often confronted with the issue of using a limited number of plants in their breeding programs, undesirable traits transferred together with the valuable resistance genes, and, in recent years, also with the depletion of potential gene sources. Control of diseases is a subject of great interest for biotechnologists. The most significant development in the area of varietal development for disease resistance is the use of the techniques of gene isolation and genetic transformation to develop transgenic resistance to fungal, bacterial and viral diseases. Improvements in genetic transformation technology have allowed the genetic modification of almost all important food crops like rice, wheat, maize, mustard, pulses and fruits. Genetic engineering technology has proved to be beneficial in managing viral and bacterial diseases in plants. The advances in gene engineering technologies and the understanding of the molecular nature of plant protection mechanisms have provided means for developing principally new strategies of plant disease control, in addition to the traditional approaches based on employing chemicals or classical breeding schemes. Biotechnology will enhance our understanding of the mechanisms that control plant’s ability to recognize and defend itself against disease caused by fungi. The integration of biotechnology with traditional agricultural practices will be the backbone for sustainable agriculture.

Mushrooming population, subsiding agricultural resources; land and water, people’s concern towards quality of vegetable s/food and changing climate throws several challenges for vegetable production. Conventional management and crop improvement strategies alone can no longer sustain the nation’s as well as global demand. So there is an urgent need to adopt modern biotechnological methods which are multidisciplinary where a large number of tools and elements of conventional breeding technique, genetic engineering, molecular biology, molecular genetics, bioinformatics and biochemistry are utilized and integrated. Biotechnological tools provide three major aspects of genetic improvement viz, application of tissue culture, molecular breeding or marker assisted breeding and genetic engineering.

Breeds are the result of the hundreds of years of natural selection and evolution. They are adapted to tropical climate and diseases and perform reasonably well even with low quality of feed and fodder. India has agrarian economy, with majority of population dependent on agriculture. India is a mega diversity hub of different livestock breeds. There are 10 buffalo breeds, 30 cattle breeds, 42 sheep breeds, 20 goat breeds, and 18 poultry breeds contributing significantly to GDP of the country. Despite our vast population of different species, there is a need for conservation of breeds. Conservation is the management of human use of the biosphere, so that it may yield the maximum sustainable benefits to the present generation, as well as maintaining its potential to meet the needs and aspirations of future generation.

Introduction

Cell and tissue culture techniques are becoming increasingly popular as alternative means of plant vegetative propagation. Plant tissue culture involves asexual methods of propagation and its primary goal is crop improvement. The success of many in vitro selection and genetic manipulation techniques in higher plants depends on the success of in vitro plant regeneration. Crop breeding and rDNA technology require the widespread use of reliable true to type propagation and better regeneration methods. Plant regeneration can be grouped in to different categories of enhanced release of axillary bud proliferation, organogenesis and embryogenesis. The oldest practical application of tissue culture techniques is the propagation of disease free plants from meristem tip culture. Meristem tip consisting of dome and one or two subjacent leaf primordial with a size up to 0.25 mm are aseptically cultured in appropriate nutrition media. The resulting plants are disease free because the pathogen is not generally present in the meristematic cells.

The aim of germplasm conservation is to ensure the availability of useful germplasm at any time. Breeding programs rely heavily on locally adapted ancient plant varieties and their wild relatives as sources of germplasm. Further, the continuing search for high yielding varieties of crop plants with resistance to pathogens and pests warrants the availability and maintenance of large collections of germplasm. In species which are propagated through seeds, it is economical to preserve the seeds. These seeds are dried to water content of 5 - 8% and then stored at a temperature of -18° C or lower and low humidity. In case of vegetatively propagated species, preservation of germplasm heavily taxes manpower and land resources. As in vitro techniques are becoming more important in crop improvement through the use of somatic cell genetics, the material produced in vitro will have to be conserved in vitro. The in vitro system is extremely suitable for storage of plant material, since in principle it can be stored on a small scale, disease free and under conditions that limit growth. Germplasm storage in vitro is crucial for the future development and safety of agriculture.

Application of computers in Hospital Pharmacy Practice
Computers have significantly transformed hospital pharmacy practices, enhancing various aspects of patient care, medication safety, and operational efficiency. Below are the key applications of computers in this field
1. Electronic Health Records (EHRs): EHRs are comprehensive digital records that replace traditional paper charts, encompassing a patient’s entire medical history. These records include data on diagnoses, lab results, medications, immunization records, treatment plans, and demographic information. EHRs offer real-time, patient-centered records accessible to authorized healthcare providers, enabling seamless sharing of information across different departments and facilities. This enhances collaboration, leading to more accurate diagnoses, coordinated care, and effective treatment plans. Additionally, EHRs support clinical decision-making through integrated alerts and reminders, ensuring critical data is communicated promptly for better patient outcomes.
2. Clinical Decision Support Systems (CDSS): Clinical Decision Support Systems (CDSS) are advanced tools integrated within healthcare IT systems to aid healthcare professionals in decision-making processes. CDSSs analyze patient-specific data and match it with extensive medical knowledge bases to provide tailored recommendations for diagnosis and treatment options. By flagging potential issues such as drug interactions, allergies, and contraindications, these systems reduce the risk of errors and enhance patient safety. CDSS can also offer reminders for preventive measures, suggest evidence-based protocols, and guide adherence to clinical guidelines, thus streamlining the clinical workflow and improving quality of care.

Purpose-To now the technique of application of thick layer sterile absorbent protective cotton padding over fractured site and its retention with thread ligature in order to protect the bone from further damage

Site-Generally area joint above and joint below of fractured site of bone needs to be covered with a thick layer for perfect immobilization of fractured bone.

Appropriate selection of fungicides and application at appropriate doses and times are of great importance in the management of crop diseases. A basic requirement of the application method is to get the fungicide to a location where the active ingredient prevents fungal damage to the plant. The fungicidal application varies according to the nature of the host part diseased and nature of survival and spread of the pathogen. The method which are commonly adopted in the application of the fungicides are discussed.

1. Foliar or vegetative application

2. Soil application

3. Seed treatment

Pre-harvest factors are responsible for the majority of post-harvest losses. Fruits which have already been infected with insects and pathogens, have been poorly irrigated and fertilized, or are of low quality before harvesting will never benefit with post-harvest cures. As a result, the process of accomplishing production quality, as well as harvesting, processing, and selling, must be taken into account.

Embryo Transfer (ET) refers to the technique by which embryos are collected from the reproductive tract of female (donor) and transferred to that of another female (recipient) which carries it to term. The donor and recipient female are in same stage of estrous cycle. The main objective of ET is the improvement of animal population through maximum utilization of superior females and males. It allows faster progeny testing of both male and females. By obtaining and transferring embryos from pre-pubertal females shorten the generation interval from birth to reproductive age. The superior buffaloes will be sent for slaughter after getting lactation yield in metropolitan cities. Utilizing oocytes for In-vitro maturation (IVM) and In-vitro fertilization (IVF) from such slaughtered superior buffaloes can prevent wastage of genetic material. ET allows preservation of rare and extinct species and breeds of animal. ET can be used as a basic tool for twinning, cloning, sexing and to test chromosomal aberrations.

Abstract

A majority of the plants studied in natural ecosystems are symbiotic with microorganisms that either reside entirely (endophytes) or partially within plants. These microorganisms express different associations ranging from mutualism to parasitism. These symbiotic relationships appear to impart tolerance to various types of abiotic stresses such as heat, drought, salinity, heavy metals, etc. and sometimes may be responsible for the survival of both plant hosts and microbial symbionts in high stress habitats. The amelioration of the abiotic stresses by the endophytes assumes increasing significance in the light of rapidly changing global climate, which is likely to face frequent incidences of extreme weather conditions like high temperature, droughts, etc.

Introduction
The conceptual foundation of environmental DNA (eDNA) can be traced back to French criminologist Edmond Locard’s principle that “every contact leaves a trace.” eDNA, often referred to as the “blueprint of life,” represents the genetic material shed by organisms into their environment. This innovative approach allows scientists to obtain biological information without directly interacting with or disturbing the organisms. eDNA technology is particularly advantageous in studying megadiverse ecosystems, as it enables the isolation and analysis of genetic material from various environmental samples, such as soil, water, and air. The approach of environmental DNA (eDNA) has its roots in metagenomics, which has long been used to study microbial communities
in soil, water, and sediment. The concept of eDNA was first applied in microbiological research on marine environments (Orgam et al., 1987; Handelsman, 2004). Early breakthroughs in biodiversity monitoring included the detection of invasive species, such as Rana catesbeiana (American bullfrog), in French swamps, demonstrating the potential of eDNA in ecological studies
(Díaz-Ferguson and Moyer, 2014). The persistence of eDNA also depends on the species and habitat: in freshwater, it may last between 15 and 30 days for fish and amphibians, while in marine environments, it typically persists for shorter durations, ranging from 0.9 to 7 days for marine mammals. These variations underscore the importance of understanding environmental and biological factors when applying eDNA methods for monitoring biodiversity and ecosystem health. The transport of eDNA in rivers is particularly rapid, with genetic material potentially traveling tens of kilometers from its source. This dynamic propagation must be accounted for when designing experiments and interpreting results.

1. INTRODUCTION

‘Fertigation’ is the technique of supplying dissolved fertilizer to crops through an irrigation system (Haynes, 1985). When efficient irrigation system combined with both nutrients and water, it can lead to obtain higher yield and preferable quality desired marketable produce.

Often, side-dressings of straight solid fertilizers are found to be the management constraints. Most of the growers have the experience of how much the applied fertilizer is either washed from the soil in run-off or leached below the root zone.

Continuous small applications of soluble nutrients have the benefits like labour, due to fertilizer being placed uniformly around the plant roots and allow savings/economy rapid uptake of nutrients by the plant.

Aim: To know the technique of application of first layer padded cloth and its thread

Key words : Integrated Watershed Management (IWM), GIS, DTM, Watershed Delination, Water Quality Modeling, RS, DSS, Ground Water Information System (GWIS).

Introduction

The availability of land and water resources has a major effect on the economic and ecological aspects of life everywhere around the world. As a result, the need to develop better tools for efficient land and water resources management has become very vital. For quicker, reliable and precise results, it is necessary to use the geospatial tools and techniques such as Global Positioning Systems (GPS), Geographic Information Systems (GIS) and Remote Sensing (RS) image interpretation and analysis techniques to manage the natural resources. The application of these tools and techniques not only facilitates timely and repetitive analysis of all the topological features, but also assists in development of Decision Support Systems (DSS) to generate alternative scenarios for optimal use of the natural resources in the long term. The optimal use of these resources through integrated management of land water and vegetation leads to enhanced production and productivity of food, fodder, fiber, fuel, fish, flower and forest resources. Moreover, the holistic management of the resources to produce these outputs is plausible and manageable within the watershed systems, which are hydro geological units from which the runoff resulting from precipitation flows past through a single outlet to downstream locations. The systematic management of land, water, vegetation and livestock resources within the watershed systems is termed as Integrated Watershed Management (IWM). Broadly, IWM can be defined as a multidisciplinary, holistic way of protecting and managing a watershed’s natural resources to enhance biomass production in an eco-friendly manner. The watershed is viewed as a hydro-geological complex and dynamic ecosystem in which natural and anthropogenic processes occur and interact resulting in generation of runoff at the watershed outlet.