eChapters

Historical Background: Mendelian Genetics
 
Gregor Mendel (1822-1884), the first geneticist, proposed his principles (Mendel’s laws of inheritance) addressing the question of ‘what is inherited and how?’ based on his experimental results with sweet pea. New scientific discoveries supported Mendel’s principles. The chromosome theory of inheritance was proposed based on the cytological observations that transmission patterns of chromosomes are closely associated with Mendel’s principles (Sutton 1903). T.H. Morgan (1866-1945) provided the empirical evidences of this theory using fruit fly, Drosophilla. He demonstrated that genes located close together on the same chromosome co-segregated in his experimental crosses (i.e. linkage), and could be mapped into linear linkage groups which corresponded to chromosomes (Morgan 1934). Thanks to Morgan’s contributions, the failure of independent assortment in Mendelian inheritance could be explained by linkage. In 1908, the equation describing the expected genotype distribution in a random mating population at equilibrium was derived by Hardy and Weinberg (Crow, 1999).

Brief History

1953: discovery of the molecular structure of DNA

1961-1965: decoding of the genetic code

1977: first method developed for DNA sequencing

1980: the principle of genetic mapping by anonymous molecular markers

1987: first “automatic” DNA sequencing machine developed

1990: publication of the BLAST algorithm for the comparison of DNA sequences

pesticides on food safety, environmental well-being, and the sustainability of agriculture have driven the search for innovative eco-friendly options that can boost crop yields, while reducing ecological damage. One such approach is the use of plant growth promoting bio-inoculants that contain beneficial microorganisms. However, conventional bio-inoculants face limitations such as poor shelf life and sensitivity to environmental fluctuations. Nano-bioinoculants address these challenges by combining beneficial microorganisms with either nanotechnology based delivery systems or nanoparticles. The incorporation of nanomaterials addresses these limitations by enhancing the stability, bioavailability and targeted delivery of microbial inoculants. In addition, these nanoparticles can act as anti-microbial agents. The combined effects of bioinoculants and nanomaterials present exciting opportunities that offer dual advantages, consequently enhancing crop yield. The application of nano-bioinoculants has shown significant promise in enhancing nutrient use efficiency by facilitating the solubilization and uptake of essential minerals. In addition, nano-bioinoculants offer effective biocontrol against phytopathogens by producing antimicrobial metabolites, competing for space and nutrients, and inducing host defense mechanisms. They are also reported to be effective for insect pest management. These nano-formulations also play a crucial role in improving plant resilience under abiotic stresses like drought, salinity, and heavy metal toxicity. The slow and targeted release of active compounds ensures long term benefits with minimal environmental

17.1 Introduction

In industrialized nations the air is filled with numerous pollutants caused by human activity or industrial processes, such as carbon monoxide (CO), chlorofluorocarbons (CFC), heavy metals (arsenic, chromium, lead, cadmium, mercury, zinc), hydrocarbons, nitrogen oxides, organic chemicals (volatile organic compounds, known as VOCs, and dioxins), sulphur and other particulates. The presence of nitrogen and sulphur oxide in the air generates acid rain that infiltrates and contaminates the soil. The high levels of nitrogen and sulphur oxide in the atmosphere are mainly due to human activities, particularly burning of oil, coal and gas. Only a small portion comes from natural processes such as volcanic action and decay of soil bacteria. Water pollution is caused by numerous factors, including sewage, oil spills, leaking of fertilisers, herbicides and pesticides from land, by-products from manufacturing and extracted or burned fossil fuels.

INTRODUCTION

Plant diseases caused by microbial plant pathogens and sub microscopic entities including virus and viroids induce both quantitative and qualitative losses of high magnitude in yields. The extent of loss may range from slight to 100% depending on the susceptibility/resistant level of the cultivars and prevailing favourable environmental conditions (Narayanasamy, 2011). With the introduction of high yielding cultivars, adoption of modern production techniques such as high input agronomic practices, changes in cultural practices, free movement of planting materials and changes in weather conditions over the years have led to increase in pest and disease problems, several of which have become major constraints in their successful cultivation (Gupta and Thind, 2006). The challenge is how to feed the growing population by producing more on a stagnant or shrinking landscape; with lesser input cost and with lesser hazards to the eco-system. The detection and management of diseases prior to economic loss will help to minimize the yield loss of the crops. Physical and chemical techniques have been applied either alone or in combination with certain cultural practices to enhance the effectiveness of reducing pathogen inoculums and consequent reduction in disease incidence.

Introduction

Nanotechnology and nanoparticles are increasingly recognized for their potential applications in various aspects of human animal and animal welfare like development of various healthcare or cosmetic products, nano-electronics and techniques for environmental remediation, and many consumer products. Nanoparticles, by definition, are structures that have one dimension in the 1–100 nm range. Nanotechnology involves the application of materials at the nanoscale to new products or processes. Over the past few decades, inorganic nanoparticles, whose structures exhibit significantly novel and improved physical, chemical, and biological properties, phenomena and functionality due to their nanoscale size, have elicited much interest. Nanophasic and nanostructure materials are attracting a great deal of attention because of their potential for achieving specific processes and selectivity, especially in biological and pharmaceutical applications. Nanotechnology has made a new generation industrial revolution by developing product and formulations for medical, agriculture (nano-fertilizers, nano-herbicides, nano-pesticides, recalcitrant contaminants from water, nano-scale carriers, nanosensors), veterinary care, fisheries and aquaculture, detection of nutrient deficiencies, preservation, photocatalysis, nanobarcode, quantum dots etc. It is a rapidly growing industry currently worth billions of U.S. dollars, with many potential benefits to society. Nanotechnology has the environmental applications to protect climate change and environmental pollution as nanocatalyst, light weight nanocomposite materials, nanocoatings, improved renewables and nanosensors.

This fast growing technology is already having a significant commercial impact, which will certainly increase in the future. Because of their widespread application, the commercial nanotechnology industry is predicted to increase significantly to more $3 trillion in few years. While nanotechnologies offer many opportunities for innovation, the use of nanomaterials in food, agriculture and environment has also raised a number of safety, environmental, ethical, policy and regulatory issues.

Introduction

Aquaculture is the fastest growing food producing sector. According to FAO (2016), Aquaculture is the farming of aquatic organisms including fish, crustaceans, mollusks, and aquatic plants in all types of environments including ponds, lakes, oceans, and rivers. Aquatic resources have played an essential role in ensuring food security by supplying the enormous demand for animal protein. Around 17% of animal protein consumed by people worldwide comes from fish (Shah and Mraz, 2020). Fish provide the highest quality protein sources, essential amino acids, fatty, vitamins, and minerals which are lacking in others (Kwasek et al., 2020). The world population will exceed 9 billion by 2040. Therefore, to feed a rapidly increasing population, aquaculture production must increase globally

Manipulation of matter at nanoscale for creating a myriad of new materials having unique optoelectronic and physiochemical attributes is called nanotechnology (Mohanpuria et al., 2008). The term nanotechnology was first coined by Drexler in 1986, in the book “Engines of creation: the coming era of nanotechnology”. In this book, he writes about the building of machines on the scale of molecules, a few nanometers size (motors, robot arm and even computers). Broadly nanotechnology refers to a field of applied science and technology whose unifying theme is the control of matter on the atomic and molecular scale, normally 1 to 100 nanometers, and the fabrication of devices within that size range. Nanotechnology is multi-disciplinary in nature often involving chemistry, biology, physics and material science. Nanotechnology is one of the budding and emerging fields of 21^st century. Nanoparticles have received increased attention in the recent past due to their unique distinct unusual physical, chemical and biological properties, which are completely different from their bulk materials and individual molecules (Li et al., 2001).

Abstract
Quality management of fish and fishery encompass the several aspects such as ensuring of food safety, preserving of freshness, promotes consumer confidence, minimize the loss and enhance the traceability. While managing the whole food supply chain becomes the most difficult endeavour. Unfortunately, biosensor emerged to fulfil the requirement of quality management in seafood supply chain and these sensors consist of detection, discrimination and avoidance of risk. Novel sensors are being origin due to evolved in every phase of requirement. The sensor has primarily performed to tracking the level of freshness or spoilage of fish product and also detection of contaminant from time of processing of fishery product to point of consumer before they eat. Recently, various biosensor based on principles of nanosensor, graphene based biosensor and evanescent wave optical biosensor arising its role in fish quality management through monitoring,and tracking of significant compound.
Keyword: Fish quality, biosensor, nanosensor, graphene based sensor, freshness, biocompounds

Introduction
Open-source software has revolutionized the way individuals and industries approach digital content creation, offering high-quality, community-driven solutions without the cost of proprietary software. Among the most popular open-source tools for graphics and 3D modeling are GIMP (GNU Image Manipulation Program) and Blender. Both programs provide powerful capabilities for creating, editing, and enhancing visual content. This chapter delves into the application of GIMP and Blender, exploring their key features, workflows, and how they can be utilized across various fields of digital production. This chapter explores the capabilities and applications of two prominent open-source software tools: GIMP (GNU Image Manipulation Program) and Blender. These software tools are transformative in the fields of digital design, animation, and media production. The chapter provides an overview of their features, real-world applications, and advantages over proprietary alternatives. Through practical case studies, the chapter
demonstrates how GIMP and Blender empower professionals, educators, and hobbyists to create high-quality visual content without incurring significant financial costs.
What is Open-Source Software?
Open-source software is freely available for use, modification, and distribution. It fosters collaboration among developers and users, resulting in cost-effective, versatile, and frequently updated tools.

Introduction

Cultivation of crops in greenhouse or protected structure has been increased tremendously in recent times and also has been spread from temperate regions to the warmer regions of tropics and subtropics. Plentiful technologies have been developed for heating the inside environment of greenhouse for optimum plant growth (Sethi et al. 2013). However, in hot arid areas, cultivation in greenhouse is very difficult as the temperature reaches to a very high level to sustain plant growth (Pek and Hayles 2004, Saran et al. 2010). Under such situation requirement for cooling the greenhouse microclimate environment is the necessity, but is very energy and water intensive. Hence, in such regions, reduction of air temperature inside the greenhouse or the regulation of temperature closer to the ambient temperature during summer is necessary for successful crop production. A typical diurnal variation of temperature and global solar radiation during late winter months inside a shade net structure has been plotted in Fig. 18.1. It has been found that global solar radiation has been decreased to almost 1/3 of the radiation received outside by using 75% shade net cover. The temperature inside structure during morning time has also been found higher than outside temperature and in summer such increase is expected to be much higher.

Therefore, cooling is considered as the basic necessity for greenhouse crop production in tropical and subtropical regions to overcome the problems of high temperatures during summer months. Development of suitable cooling system that provides congenial microclimate for crop growth is a difficult task as the design is closely related to the local environmental conditions. Different cooling techniques for greenhouse crop cultivation may be found in Kumar et al. (2009). Fan pad cooling system has been widely used for greenhouse cultivation however, requires large amount of water during evaporation based cooling. Since water resources are becoming scarce in future such fan-pad cooling system may not be viable in future specifically in hot arid region.

Fracture in animals occur frequently and is a concern of economic importance to the owner. The practice of keeping them as pets in households rather than in sheds or in confinements have increased the chance of encountering accidents like fall from coat, terrace, limbs being trapped in drains, being hit by vehicles, dog bites etc. leading to fracture. Fracture management in small animals/small ruminants can be successfully achieved owing to their light weight; they tolerate external coaptation relatively well and can ambulate in three limbs well in order to spare the injured limb. Fracture can be repaired successfully with regional anaesthetic techniques which they can tolerate very well. The constraint of cost effectiveness can be balanced by carefully selecting the appropriate technique according to the weight and limb involved. Many economical skeletal fixation technique have been evolved which can well fit into the budget of the owner. Knowledge of simple techniques can enhance healing in cases of bone loss and management of infection.

Fracture site stabilization

Fracture repair is usually elective, hence initial support and care of the fracture is necessary to prevent further trauma and complications like communication of fracture site to the exterior through wound or sinus and chances of infection. Initial support to splint the bone not only limits the movements but also supports the soft tissues from untoward consequences. Limb splinting reduces edema and pain, preserves neurovascular supply to the limb till the day of operation and helps to transport the animal safely. Fracture to limbs caudal to mid radius ulna/ tibia can safely be stabilized by splints or full limb casts. Wood, pliable bamboo or split poly vinyl chloride pipes positioned on the caudal and lateral aspects of the limbs and supported firmly with tapes or bandages can serve the purpose. Fracture proximal to mid radius ulna or tibia should not be stabilized using splits since the fulcrum effect created due to this can augment soft tissue injury. Careful assessment of the viability of the structures caudal to fracture site should be assessed prior to a decision regarding surgery, devitalized tissue hamper healing and is a subject to be considered for amputation.

Introduction

Prebiotics are non-digestible food ingredient which beneficially affects the host by selectively stimulating the growth and/or activity of one or a limited number of health-promoting bacteria in the intestinal tract, thus improving the host‘s microbial balance (Gibson and Roberfroid, 1995). Normally, prebiotics contain some type of a non-digestible carbohydrate fraction (Rehman et al., 2009). Almost 90% of oligosaccharides escape digestion in the small intestine and reach the colon where they selectively stimulate the growth and/or activity of beneficial bacteria like Bifidobacteria, Lactobacillus and Streptococcus, providing prebiotic properties (Stanton et al., 2003). These bacteria populate the gut filling any niches were pathogenic bacteria may attempt to take hold. This concept is better known as competitive exclusion. Prebiotics function by lowering the gut pH through lactic acid production, inhibiting/preventing colonization of pathogens, modifying metabolic activity of normal intestinal flora, and stimulation of the immune system (Doyle and Erickson, 2006). Studies have demonstrated that these bacteria reduce pathogenic bacteria like Salmonella spp., Clostridium diffile and Campylobacter spp. (Hopkins and Macfarlane, 2003; Stern et al. 2000), by diverse mechanisms including competitive exclusion of nutrients or adhesion sites to intestinal mucosa (Stern et al. 2000) and fatty acid of short chain production, like propionic and butyric acids, which modifies the acidic conditions and inhibits the growth of microorganisms that cause infections (Collins and Gibson, 1999).

Radionuclides are used in two major ways: for their chemical properties and as sources of radiation.

Radionuclides of familiar elements such as carbon can serve as tracers because they are chemically very similar to the non-radioactive nuclides, so most chemical, biological, and ecological processes treat them in a near identical way. One can then examine the result with a radiation detector, such as a Geiger counter, to determine where the provided atoms ended up. For example, one might culture plants in an environment in which the carbon dioxide contained radioactive carbon; then the parts of the plant that had laid down atmospheric carbon would be radioactive.

In medicine, radioisotopes are used for diagnosis, treatment, and research. Radioactive chemical tracers emitting gamma rays that can provide diagnostic information about a person’s internal anatomy and the functioning of specific organs. This is used in some forms of tomography single emission computed tomography and positron emission tomography scanning. Radioisotopes are also a promising method of treatment in hemopoietic forms of tumors, while the success for treatment of solid tumors has been limited so far. More powerful gamma sources are used to sterilize syringes and other medical equipment. About one in two people in Western countries are likely to experience the benefits of nuclear medicine in their lifetime.

In biochemistry and genetics, radionuclides are used to label molecules and allow tracing chemical and physiological processes occurring in living organisms, such as DNA replication or amino acid transport.

Introduction

After the Second World War, isotopes have become available in sufficient quantities for use in different fields of scientific research. There has been tremendous increase in the number and scope of applications of these new tools and agricultural research is no exception. During the last quarter of a century, the use of isotopes and radiations has become one of the important tools in the solution of practical problems in the area of soil-plant nutritional studies throughout the world. In India, these studies were initiated as early as 1955 with the establishment of the Radiotracer Laboratory at the Indian Agricultural Research Institute, New Delhi. The use of these nuclear tools got further fillip in our country with the establishment of the Nuclear Research Laboratory at the Indian Agricultural Research Institute, New Delhi in 1968 as a UNDP Project on Nuclear Research in Agriculture, operated by the Joint FAO/IAEA division of the International Atomic Energy Agency. Also, the agriculture division of Bhabha Atomic Research Centre, Mumbai and the special radioisotope laboratories established by several agricultural universities had made significant contributions to the use of isotopes and radiations in agricultural research.

Introduction

Genetic markers have assisted in genomic characterization of various crops species (Rafalski and Tingey, 1993). Most of the genetic marker systems rely upon DNA sequence polymorphisms (Staub et al., 1996). Each system has unique advantages and disadvantages based on cost, experimental objectives and type of information desired. Regardless of its inherent methodology, the effective use of any marker system for genetic analysis requires reliability and consistency.

The random amplified polymorphic DNA i.e. RAPD (Williams et al., 1990) or arbitrarily primed polymerase chain reaction (Welsh and McClelland, 1990) marker identifies DNA sequence polymorphisms. In this technique, segments of genomic DNA are amplified in a polymerase chain reaction i.e. PCR (Mullis et al., 1986) with random primers followed by agarose gel electrophoresis. This is simple, relatively inexpensive and is amenable to high throughput investigation. RAPD markers are usually dominant and polymorphisms are observed as the presence or the absence of DNA bands of a particular size. RAPDs are common molecular approaches employed in plant breeding, genotypic differentiation, gene linkage analysis, assigning evolutionary and taxonomic affinities and relatedness and DNA fingerprinting analysis (Lin et al., 1996).

ABSTRACT

The discovery of polymerase chain reaction method of amplification of DNA is an important milestone in molecular genetics and used for multiple purpose such as construction of linkage maps, marker saturation of specific genome regions, analysis of genetic diversity, molecular phylogeny and cultivar identifications (Williams et al; 1990, Demeke, Adams and Chibbar; 1992, Welsh and Maclelland; 1990;, Obara-Okeyo and Kako; 1998, Sawhoon Lin et al; 1998 and Grunger et al; 1998.). 15 species of three morphologically distinct groups of Orchids belonging to the subfamily Epidendroideae and tribe Dendroebieae and Cymbideae were studied using random amplified polymorphic DNA (RAPD) technique. Genetic variability and relation between these 3 genera and 15 species were estimated based on band sharing and cluster analysis. A total of 227 distinct major RAPD bands of which 97% were polymorphic were generated from 15 arbitrary primers. The number of bands generated per primer ranged from 3 (Primer A-10) to 22 (Primer K-10 and N-17) with a mean of 14.8 bands per primer. Out of 227 bands generated 222 bands (97.8%) where polymorphic while only 5 bands were monomorphic. The primer resolving power was found to range between 2.00 (prime A-10) and 13.38 (primer k-10) when all the species were studied were taken into account. The molecular analysis grouped all the species into 5 groups. The polymorphic patterns generated by RAPD profiles showed different degrees of genetic relationship among the species studied and the RAPD marker were found to be an useful tool for detecting genetic variation within the species of three important genera of Orchids.

Ali Demirci et al., (2014) studied the reactors with biofilms which are used to produce many value added products. These include organic acids, alcohols, enzymes, antibiotics, bacteriocins and many more. Many bioreactor designs favor the biofilm formation in a reactor. The biofilm reactors are utilized for fermentation and bioconversion of large amount of biomass. Recently, the biofilm reactors have been applied for production of many value-added products with novel designs. According to Ercan and Demirci (2015) the biofilms can cause both detrimental and beneficial effects in the environment. Biofilms in bioreactors has many advantages due to the membrane fouling which reduces the cost. Higher biomass density can be utilized and the operation is more stable.Biofilm reactors are commercially being utilized for waste water treatment, bench and pilot-scale production of value-added products. In order to run the biofilm reactor at optimum conditions it is important to understand the fundamentals of biofilm formation, the different properties

1. Introduction

Aquaculture is described as an organized production of crops in an aquatic medium (FAO, 1987). Global aquaculture production has continued to expand at approximately 9% per year since the 1970s, with Asia dominating production levels, particularly for finfish (FAO, 2014). FAO data indicate that global fish production stands at 167 million tons (MT), of which 44% (73.8 MT) is contributed by the aquaculture sector (FAO, 2016). Twelve percent of the world's population depend on the fishing industry for their livelihood as and it provides 17 percent of animal protein. India is second only to China in terms of the world's output of culturable fisheries. According to reports, the aquaculture industry in India has suffered significant losses from the frequent incidence of infectious diseases of bacterial and viral origins, which has hindered sustainable development. To overcome the challenges of developing appropriate technologies for managing water quality in culture systems, developing quick diagnostic tools and ways to contain disease outbreaks, and providing disease-free or highly healthy broodstock and seeds, aquaculture requires creative biotechnological interventions “is the need of the hour” (Subasinghe et al., 2003).

The application of (VCRS) Vapour compressor refrigeration system is in the air conditioners (window, split, central or precision), car air conditioners, domestic refrigerator etc. The different parts of a domestic refrigerator are: in the hermetical sealed compressor, air cooled condenser, capillary tube, thermo expansion device, filter, rotameter, solenoid valve, temperature control sensor.