eChapters

Any phenotype is the result of interaction of genotype and the environment. Any genotype cannot express itself unless proper environment for its expression is made available. Likewise, environment cannot create any phenotype unless a genotype is there to be acted upon by the environment. However, the effect of genotype and the environment is not fixed, its keeps on changing depending upon the relative role of the genotype. Heritability therefore specifies the proportion of the total phenotypic variability that is due to genetic causes. In other words, heritability is defined as the ratio of genetic variance to the total phenotypic variance. Heritability is the degree to which the characteristics of a plant are repeated and expressed in the progeny. This can be expressed quantitatively as follows:

Heritability (h²) = Genotypic variance/Phenotypic variance

Initially, exploitation of heteosis was confined to corn and a few other open- pollinated crops but presently heterosis is being exploited practically in all species cutting across mode of reproduction. More and more evidences are emerging that heterotic expression in a cross is a function of genetic backgrounds and genes. Heterotic grouping would remain a key strategy in management of diverse genetic backgrounds and genes for full exploitation of heterosis. The commonly used methods in developing hybrids in self- pollinated crops are listed and explained as follows:

Manual emasculation and Pollination Method

In this method, female parent flower buds are emasculated manually and the emasculated buds are pollinated by the pollen grain collected from protected flowers of the male parent. This method is effective and successful where the crossed fruits produce plenty of seeds and the seed requirement for planting the commercial hybrid seed is much less. Consequently, this method is being used in a routine manner on a commercial scale in vegetable crops mostly by private sector seed companies in India and south-east Asian countries. The crops covered under this method of hybrid seed production are as follows:

Tomato, hot-pepper, sweet pepper, eggplant, okra, bottle gourd, bitter gourd, ridge gourd, smooth gourd, cucumber, pumpkin, water melon and muskmelon. Cucurbits are included here as although they are cross-pollinated but behave like self-pollinated crops in terms of almost no inbreeding depression.

Chi-Square

The Chi-square test (c₂) proposed by Karl Pearson in 1899 is used to measure goodness of fit of observed and theoretical or expected data and is a continuous distribution.

Formula for Chi-Square

1. General Formula

The general formula for Chi-square is as follows

Translocations

Chromosomes occasionally undergo spontaneous rupture or can be induced to rupture in high frequency by ionizing radiation. The broken ends of such chromosomes behave as though they were ‘sticky’ and may rejoin into non- homologous combinations giving rise to translocations. The most common type of translocation is reciprocal translocation which involves the exchange of segments between two non-homologous chromosomes. Translocations change only the arrangement of genes in the chromosomes, not the quality or quantity of genes. For this reason, they are sometimes referred to as chromosomal rearrangements. Individuals carrying such rearrangements should be phenotypically entirely normal unless the relations of a gene or genes to adjacent genes affect the phenotypic expression (position effect). The first translocation was discovered by Bridges in 1923 in D. melanogaster.

The genetic techniques for detecting and studying translocations will be more easily understood when the cytological phenomena produced by translocations are known. Suppose two chromosomes having respectively the genes ABCDEF and GHIJKL exchange sections and give rise to translocation chromosomes ABCJKL and GHIDEF. The individual thus formed receives from one of the parents the normal and from the other parent the translocation chromosome. Such an individual is a translocation heterozygote (Fig. 36.1).

Watson and Crick based on their double helical DNA model, offered to explain DNA replication by a method where each single strand of DNA is template or mold for its complement and a new helix has one old strand and one newly synthesized strand. This type of DNA replication is called as semi-conservative as in this method one old strand is retained and one new complementary strand is synthesized. In contrast to semi-conservative mode of DNA replication, two more modes of DNA replication were proposed. One is conservative type where two new strands are synthesized in form of a double helix while the old double helix remains unchanged. The third type of DNA replication proposed has been dispersive mode of DNA replication which amounts to breaking down of double helical strands into small pieces along the length, their replication and random assemblage with newly synthesized pieces to form a patchwork single string of dispersed old and new pieces. These three modes of replication of DNA molecule are shown in Fig. 40.1.

In transcription, it has become clear as to how there is the transfer of genetic information stored in the sequence of nucleotides-pairs in DNA to the sequence of nucleotides in m-RNA molecules which carry that particular information from the nucleus to the sites of protein synthesis (ribosomes) in the cytoplasm. Further, there is the requirement of the process by which genetic information stored in the sequences of nucleotides in m-RNAs is used to specify the sequences of amino acids in the polypeptide gene products. This process known as translation takes place in the cytoplasm on complex workbenches called as ribosomes. With exception of water, proteins are by far the most prevalent component of living organisms in terms of total mass.

Proteins (= enzymes = genes) are composed of polypeptides and every polypeptide is encoded by a gene. Each polypeptide has a long sequence of amino acids linked together by covalent bonds. There are 20 different amino acids (glycine, alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan, cysteine, serine, threonine, tyrosine, asparagine, glutamine, aspartic acid, glutamic acid, lysine, arginine, and histidine) present in most proteins. All amino acids except proline contain a free amino group (NH₂) and a free carboxyl group (COOH) as illustrated below:

Abstract

Pigeonpea (Cajanus cajan) is an important pulse crop of India and its production is greatly affected by wilt disease. Pigeonpea wilt is a very destructive soil borne disease caused by Fusarium udum. In the experiments, ten treatments were applied viz.; Neem leaf extracts (T₁), Garlic extracts (T₂), Tulsi leaf extracts (T₃), bio-agents Trichoderma viride (T₄), Pseudomonas fluorescens (T₅), Polymer coating (polycot) @ 4 ml diluted in 5 ml of water per kg of seed (T₆), Polymer coaating + Trichoderma viride @ 6.0 g per kg seed (T₇), Polymer + Pseudomonas fluorescens @ 6.0 g per kg seed (T₈), Polymer coating (polycot) 4 ml diluted in 5 ml of water per kg seed + Vitavax power @ 3g per kg seed (T₉) and T₁₀ served as untreated. The observations were recorded six times at 10 days intervals and started from 40 days. These polymer alone @ 4.0ml per kg and in combination with bioagents and fungicides were applied to seed were tested against Fusarium udum. The highest per cent disease control and per cent wilt disease incidence were recorded 96.09 to 64.09 and 2.74 to 33.32, respectively in T₉ (Polymer + Vitavax power @ 3g per kg seed) at 10 days intervals followed by T₈ (Polymer + P. fluorescens) gave 94.46 to 53.06 per cent inhibition, T₇ (92.39 to 52.74) and T₆ (90.97 to 49.73) however, the lowest per cent disease control was noticed as 76.19 to 40.13 per cent in T₁ (Neem leaves extract) amongst all treatments except untreated.

Abstract

Pigeon pea is an important pulse crop of India and its production is greatly affected by wilt disease. Pigeon pea wilt is a very destructive soil borne disease caused by Fusarium udum. In present research work plant extracts (Garlic, Neem, Tulsi), bio-agents (Trichoderma viride and Pseudomonas fluorescens) and fungicides (Vitavax, Bavistin and Thiram + Carbendazim) were tested against Fusarium udum. Among them fungicides were found most effective in inhibiting mycelial growth of Fusarium udum recorded 100% inhibition followed by garlic bulb extract (52.12 to 56.33%). Among bioagents, Pseudomonas fluorescens inhibit the growth up to (46.00 to 51.79%) followed by T. viride (44.32 to 48.79%) amongst all treatments except untreated.

Keywords: Pigeon pea, Fusarium udum, bio-agent, fungicide

Pigeon pea (Cajanus cajan L. Millsp.) is one of the major pulse crops grown in the tropics and subtropics belonging to family Leguminaceae. It has wide adaptability and low input requirements mostly grown in kharif season.

Abstract

Lentil is an important pulse crop and play very important role in the supply of the protein to under nourished vegetarian population of the country. Fusarium wilt of lentil caused by Fusarium oxysporum f. sp. lentis is one of the wide spread and destructive diseases in India under rice-based cropping system. Use of synthetic fungicides has led to the emergence of several problems like environment pollution, residual effect in grains and killing of non-target organism(s). Hence, for minimizing the losses caused by Fusarium wilt, four plant extracts such as Garlic, Neem, Eucalyptus, Tulsi and three antagonistic bio-agents viz. Trichoderma viride, Trichoderma harzianum and Pseudomonas fluorescence were evaluated in vitro. The results clearly showed that all plant extracts were more or less inhibitory to mycelial growth of Fusarium oxysporum f. sp. lentis. The effectiveness of extracts increased with increase of their concentration and maximum growth inhibition was obtained in Eucalyptus followed by Garlic and Tulsi. While minimum per cent inhibition was recorded in Neem. Among bio-agents, T. viride showed maximum growth inhibition of Fusarium oxysporum f. sp. lentis followed by T. harzianum at both 4 and 7 days incubation period.

Abstract

Potato (Solanum tuberosum L.), an important cash crop of India, is prone to many diseases and amongest these, late blight [Phytophthora infestans (Mont) de-Bary] is one of the highly destructive diseases and a major constraint in the profitable cultivation of potato. After realizing the significance of controlling the devastating disease for the sustainability in potato production and thus ensuring more profitability, the present investigation has been conducted with an objective to select the most effective fungicides and botanicals for the management of the disease. The present study was carried out by KVK, Saharsa during rabi season for consecutive two years (2015-16 and 2016-17) as On-Farm Trial mode at farmers fields of an adopted village of Saharsa district in Bihar. In each trial, there were four treatments including one control i.e. farmers’practice. The study concludes that the best treatment option to combat late blight disease is Seed treatment with Trichoderma viride @ 5g per kg of seed and spray of Ridomil @ 2g per litre of water starting from 30 DAP alternating with Dimethomorph 1g + ridomil 2g per litre of water at 10 days intervals. The results of economic analysis reveal that the net return as well as benefit cost ratio was also highest in Seed treatment with Trichoderma viride @ 5g per kg of seed and spray of Ridomil @ 2g per litre of water starting from 30 DAP alternating with Dimethomorph 1g + ridomil 2g per litre of water at 10 days intervals.

Abstract

India ranks first among world’s mango producing countries accounting for about 50 % of the world’s total mango production. In Bihar it is the most important fruit crop as well as occupies a large area affecting socio economic conditions of the region. The crop suffers from over 140 diseases all over the world. From young seedlings to mature trees and fruits in their all stages from start to storage may suffer from a number of biotic and abiotic stresses. All parts of the plant namely, trunk, branch, twigs, leaf, petiole, flower and fruits are prone to attack by a number of pathogens The changes in climatic patterns and shifting towards commercial cultivation of this crop has seen the emergence of some diseases which can cause huge losses to the production of mango over the years. One such problem, decline in mango is reported to be a complex problem involving multitude of factors. Decline disorders are recognized in virtually all mango-producing regions of the world. In most cases, the disease has been characterized by leaf drooping and drying; bark splitting, gum secretion from branches and main trunk, bud necrosis, dieback, vascular discoloration and death of the whole tree. The bark beetle Hypocryphalus mangiferae is closely associated with trees affected by mango sudden decline disease. Report suggests the causal agent to be Botryodiplodia theobromae, Ceratocystis manginecans, Fusarium spp, Colletotrichum sp, Pestalosiopsis maniferae. In most of the cases Botryodiplodia theobromae, Ceratocystis spp are associated with it. Although, none of the pathogens isolated could singly incite the complete decline symptoms under artificial inoculation on mango seedlings indicating towards complex etiology of the disease. Since pathogens are endophytic in nature; mangement of the pathogen after infection is difficult and relies more upon prohytalctic measures. Over all plant health, its nutritional status and abiotic conditions of flood and drought are also the pre disposing factors to decline in Mango. Effective management of decline in mango needs holistic approaches using optimum nutrition, general sanitation, routine crop protection schedule, weed management and appropriate intercropping practices.

Proteins

Proteins are of central importance in the structure and function of all living organisms. Some proteins serve as structural components while others function in communication, defense, and cell regulation. Some proteins serve as enzymes that act as biological catalysts which control the pace and nature of essentially all biochemical events. Although DNA serves as the genetic blueprint of a cell, none of the life processes would be possible without the proteins.

Electrophoresis of Proteins

Proteins can be separated and purified. Methods for separating proteins take advantage of properties such as charge, size, and solubility, which vary from one protein to the next. Because many proteins bind to other biomolecules, proteins can also be separated on the basis of their binding properties. The source of a protein is generally tissue or microbial cells. The cell must be broken open and the protein must be released into a solution called a crude extract. If necessary, differential centrifugation can be used to prepare subcellular fractions or to isolate organelles. Once the extract or organelle preparation is ready, a variety of methods are available for separation of proteins. Ion-exchange chromatography can be used to separate proteins with different charges (similar to the way amino acids are separated). Other chromatographic methods take advantage of differences in size, binding affinity, and solubility. Nonchromatographic methods include the selective precipitation of proteins with salt, acid, or high temperatures. In addition to chromatography, another important set of methods is available for the separation of proteins, based on the migration of charged proteins in an electric field, a process called (gel) electrophoresis. Gel electrophoresis is especially useful as an analytical method. Its advantage is that proteins can be visualized as well as separated, permitting a researcher to estimate quickly the number of proteins in a mixture or the degree of purity of a particular protein preparation. Also, gel electrophoresis allows determination of crucial properties of a protein such as its isoelectric point and approximate molecular weight.

There are many different methods of extracting DNA bands from an agarose gel. Which one you choose will probably depend on the consumables you have available in your lab. Another important consideration is the yield/ purity of the DNA after extraction. Agarose contains various impurities which may inhibit downstream reactions if not efficiently removed from the DNA. We used to give the generic term ‘hoonose’ to the unspecified carbohydrates that co-purified along with the DNA and inhibited enzyme reactions. Modern spin-column kits are very good at removing impurities, old-fashioned ‘kitchen sink’ methods are less good.

Cutting Out the DNA Band

Most of the methods require you to cut out the band. You must visualise the band, in an ethidium bromide stained gel, in a dark-room on a UV light- box (a trans-illuminator). UV is dangerous, wear gloves, long-sleeves and face protection. Never look at UV with unprotected eyes. If possible, set the trans-illuminator to long-wavelegth UV (or low-power) and minimise the amount of time the DNA is exposed. This is because the UV mutagenises the DNA at a measurable rate. Use a scalpel blade to cut around the band of interest. Switch off the transilluminator, switch on the white light and carefully remove the band from the gel and place it on the glass. It is good to trim off as much empty agarose as possible so go back to UV illumination briefly to do this. Place the excised band in a 1.5mL microfuge tube.

Restriction Enzymes

Restriction enzymes are enzymes isolated from bacteria that recognize specific sequences in DNA and then cut the DNA to produce fragments, called restriction fragments. Restriction enzymes play a very important role in the construction of recombinant DNA molecules, as is done in gene cloning experiments. Another application of restriction enzymes is to map the locations of restriction sites in DNA.

Restriction endonucleases, often called restriction enzymes, cut DNA within the molecule by hydrolyzing the phosphodiester bonds between the nucleotides. Restriction enzymes were discovered in bacteria and there are now more than 1200 known restriction enzyme types. These enzymes are named using a simple system. EcoRI, for example, was isolated from E. coli and was the first enzyme isolated from a particular strain, hence the designation of I. HaeIII was isolated from Haemophilus aegyptius. HindIII was isolated from Haemophilus influenzae, and was the third enzyme discovered in a particular strain.

Usually, restriction enzymes only cut the DNA at or near a very specific nucleotide sequence known as a recognition site. This “restrictive” nature of these enzymes allows molecular biologists to pin-point exactly where the DNA is to be cut. In many cases, the recognition site of a restriction enzyme consists of a palindromic sequence where the order of the bases is read the same on each side of the helix. The two sides of the helix are antiparallel and the recognition sequence reads from 5’ to 3’ on either side of the helix. For example the recognition site for EcoRI is as follows;

Introduction

The AFLP amplified fragment polymorphism also known as selective restriction fragment amplification (SRFA) technique is used to visualize hundreds of amplified DNA restriction fragments simultaneously. The AFLP band patterns, or fingerprints, can be used for many purposes, such as monitoring the identity of an isolate or the degree of similarity among isolates. Polymorphisms in band patterns map to specific loci, allowing the individuals to be genotyped or differentiated based on the alleles they carry. produces highly complex DNA profiles by arbitrary amplification of restriction fragments ligated to double-stranded adaptors with hemi-specific primers harboring adaptor-complementary 5' termini.The technique has been widely used in the construction of genetic maps containing high densities of DNA marker loci.

The AFLP protocol amplifies restriction fragments obtained by endonuclease digestion of target DNA using “universal” AFLP primers complementary to the restriction site and adapter sequence. However, not all restriction fragments are amplified because AFLP primers also contain selective nucleotides at the 3' termini that extend into the amplified restriction fragments. These arbitrary terminal sequences result in the amplification of only a small subset of possible restriction fragments. The number of amplified fragments can therefore be “tailored” by extending the number of arbitrary nucleotides added to the primer termini. Alternatively, the use of endonuclease combinations that vary in their restriction frequency can also be used to tune the number of amplicons. Generally, the abundant restriction fragments produced from complex genomes require of AFLP primers with longer selective regions. Conversely, analysis of small genomes require of only few arbitrary nucleotides added at the primer 3' termini. The resulting AFLP fingerprints are usually a rich source of DNA polymorphisms that can be used in mapping and general fingerprinting endeavors.

Introduction

Southern blotting was named after Edward M. Southern who developed this procedure at Edinburgh University in the 1970s. To oversimplify, DNA molecules are transferred from an agarose gel onto a membrane. Southern blotting is designed to locate a particular sequence of DNA within a complex mixture. For example, Southern Blotting could be used to locate a particular gene within an entire genome. The amount of DNA needed for this technique is dependent on the size and specific activity of the probe. Short probes tend to be more specific. Under optimal conditions, you can expect to detect 0.1 pg of the DNA for which you are probing.

The biological diversity of each country is a valuable and vulnerable natural resource. Sampling, identifying, and studying biological specimens are among the first steps toward protecting and benefiting from biodiversity. Knowledge about biodiversity and the ability to identify organisms that comes with it are global public goods. That is, this knowledge can create benefits for all countries - benefits that no one country can create by itself.

DNA Barcoding: Documenting Biodiversity with a Gene Sequence

“DNA barcoding” is an exciting new tool for taxonomic research. The DNA barcode is a very short, standardized DNA sequence in a well-known gene. It provides a way to identify the species to which a plant, animal or fungus belongs. The Consortium for the Barcode of Life (CBOL) is promoting international partnerships that will enable people in all countries to better understand and protect their biodiversity.

DNA barcoding is a taxonomic method that uses a short genetic marker in an organism’s mitochondrial DNA to identify it as belonging to a particular species. It is based on a relatively simple concept: most eukaryote cells contain mitochondria and mitochondrial DNA (mtDNA) has a relatively fast mutation rate, which results in significant variance in mtDNA sequences between species and, in principle, a comparatively small variance within species.

NTSYSpc 2.0 software is used for various types of analysis including calculation of similarity index values and construction of dendrogram. Construction of dendrogram using gel data in the form of bands. This software accepts zero-one data.

Botany

Several vernacular names of kewda are reported in different languages all over the world ; viz.

Keora, Ketgi, Keura, Gagandhul in Hindi; Keya, Ketaki, Kea, Ketkikea in Bengali; Kiya, Kia in Odia; Kewoda in Gujrati; Ato, Keto, Covasso in Konkani; Keoda, Keora in Marathi; Talemara in Kannada; Gedaji, Gedangimogali, Gojjangi, Ketaki, Mugali, Mogali in Telugu; Kaidai, Talhai, Thasai, Thazh, Kechiya, Kedagi, Kedagai, Muchali in Tamil; Kaita, Ketaki, Palliyambu in Malayalam; Gandhapuspa, Dalapushpa, Shivadvishta, Ketaki, Suchika pushpa in Sanskrit; Leram in Nicoberese; Mudukeyiya, wetakey in Sinhalese; Keora in Urdu; Gulkiri, Kader, Kadi in Persian; Satthapu, Tsatthapu in Burmese; Nalu in Nepalese; Togenashiadan in Japanese; Kadar, Kadhi, Kazi in Arabic Kaide, Kedige, Ketaki, Gedege, Mundige in Canarese; Kedgi in Deccan; Vaquois odorant in French and Schrauben palme in German. (Kirtikar and Basu, 1935; Randhawa and Mukhopadhyay, 1986; Kapoor, 1990).

Soil

Soil has a pronounceed effect on the plant growth and productivity in kewda. Well drained sandy-loam soil with adequate supply of moisture is best suited for its vigorous growth and flower production. Thickly distributed profitableKewda plantations with normal flowering are found in the lateritic belts with deposits of silty loam soil and adequate moisture supply. In heavy clay soil with inadequate moisture supply, the plants grow vegetatively with poor flowering. At places close to the Bay of Bengal like Rangiagarh, Kujang, Paradeep of Jagatsinghpur district, where a silt-loam soil deposit is found over the clay zone, the dense plantations of kewda with free flowering ability are noticed. But surprisingly, the female plants are more at few places close to the Paradeep port, which may be due to seed propagation by drifting of ripe fruits from the uplands; while in Ganjam district, the farmers are used to plant the selected male plants in their field resulting in high flower production.

The tentative age of the natural plantations is estimated from the rates of leaf production, stem and branch growth (5-50 cm / year) and development of branching pattern (primary and secondary) in conjuction with the interactions made with the local farmers of kewda (Fig. 9). The plants of different age groups (1 to 5 years) are observed for growth characters and flower production at Tulu of Ganjam and Konark of Puri districts under farmers’ field condition and it is observed that the initiation of flowers started at 5 years of age and the rate of flower production was 1.7 to 2.2 per plant (Table 9).

I. General information

1. Lalbagh garden is located at Bangalore
2. Founder of Lalbagh garden is Hyder Ali and his son Tipu Sultan
3. Total area of Lalbagh garden is 50 hectares and developed during 1760
4. Brindavan garden is located at Mysore
5. Government botanic garden, Ooty was established during 1848
6. Total area of Ooty botanic garden is 20 hectares

I. General information

1. Bonsai is defined as Japanese art of growing trees & shrubs by extreme dwarfing in a shallow container
2. Bonsai, the name is derived from two words, Bon & Sai
3. The meaning for the word ‘Bon’ is tray or shallow container
4. The meaning for the ‘Sai’ is to grow
5. Origin of Bonsai is china
6. In Mamie bonsai, plant height is 2-6 inches
7. In small bonsai, plant height is 6-12 inches
8. In medium bonsai, plant height is 12-24 inches

Importance of Rainfed Farming

In India, rain dependent areas are vast and they have a great contribution to make in agricultural production, with just 1/40th of the world land. Of the 141 M ha of net sown area in the country, 80 M ha is rainfed. Rainfed agriculture contributes 40% of food grain production and supports 2/3rds of the livestock population. Despite considerable progress in irrigation development over the five year plans, 85% of coarse cereals, 83% in pulses, 42% in rice, 70% in oilseeds and 65% in cotton are still cultivated as rainfed. A large portion of industrially important crops such as cotton, groundnut and castor are cultivated under dry land conditions and has a great contribution to make to the production of food, fibre, fuel and furniture timber etc. Though, impressive gains were noted in some of the rainfed crops in recent times, the gap between attainable and farmers’ yields still remain high which is a major cause of concern. Small and marginal farmers who are the backbone of rainfed farming are resource poor and risk averse. Moreover, both public and private investments in technology adoption and infrastructure have been quite low in rainfed agriculture resulting in a vicious circle of low yields-low net returns-low investments in improved technologies.

With several resource management problems emerging in irrigated regions, rainfed agriculture offers scope to contribute to the growing food needs of future particularly of pulses and edible oils. The high yield gaps between attainable yields realized by progressive farmers using improved technologies and those actually realized by the farmers at large in any given region offer scope for enhancing overall productivity of rainfed crops anywhere between 50 to 70% in the near term with the adoption of the available technologies, provided resource and institutional constraints in delivering the technology and inputs are addressed timely and adequately. With the promised break-through in yields through genetic manipulation and large scale adoption of water saving technologies through conservation agriculture, the yield gains can be further enhanced in the medium to long term.

The 80 million hectares of rainfed farming area is not uniform for resource base, opportunities and constraints. There are areas receiving high rainfall and fertile deep soils where crop yields can be enhanced significantly by double cropping combined with supplemental irrigation with water harvesting. For the relatively lower rainfall regions, the overall system productivity needs to be enhanced by integrating crops, trees and livestock. This agro-eco region based differential approach is the key for realizing the full potential of rainfed agriculture besides addressing the dimension of regional equity.

Management of watershed in India

It involves management of water, land, energy and greenery, integrating all the relevant scientific approaches appropriate to socio-economic background for a pragmatic development of a watershed. In practice, it means allowing load free rainfall run off from the watershed. Clear outflow imply management of effective soil and water conservation and generating a lush green carpet in whole of the area. In other worlds, it is greening of a watershed through proper management of land, water and energy sources. The comprehensive development of watershed seems to make productive use of all its natural resources and also protect them is termed as watershed management.

The Banave terraces in Indonesia and those in Nepal, Mediterranean are examples of integration of human elements into a system of land management, which is an early achievement in watershed management. Watershed management is not merely anti-erosional and anti-run off approach but also a comprehensive integrated approach of land and water resources management. The approach is preventive, progressive, corrective as well as curative. Here wasted land resources could be fruit fully harvested into a productive face for rural development.

Integrated watershed management programme (IWMP)

The main objectives of the IWMP are to restore the ecological balance by harnessing, conserving and developing degraded natural resources such as soil, vegetative cover and water. The outcomes are prevention of soil run-off, regeneration of natural vegetation, rain water harvesting and recharging of the ground water table. This enables multi-cropping and the introduction of diverse agro-based activities, which help to provide sustainable livelihoods to the people residing in the watershed area. In addition, there is a Scheme of Technology Development, Extension and Training (TDET) is also being implemented to promote development of cost effective and proven technologies to support watershed management.

Till 1.4.2008, Department implemented 3 watershed programmes viz. Integrated Wastelands Development Programme, Drought Prone Areas Programme, Desert Development Programme. Since then, they have been brought under a comprehensive programme named Integrated Watershed Management Programme (IWMP) to be implemented under Common Guidelines on Watershed Development, 2008.

Introduction

We are blessed with solar energy in abundance at no cost. The solar radiation incident on the surface of the earth can be conveniently utilized for the benefit of human society. Solar energy can be used as thermal energy for water heating, cooking, drying, distillation, space heating, cooling and power generation or it can be converted to electricity through photovoltaic cells, commonly known as solar cells. One of the popular devices that harness the solar energy is solar hot water system (SHWS). Flat plate collectors are used for low temperature applications (below 100^oC) while concentrators are preferred for higher temperatures. The most efficient application of flat plate collector is for getting hot water. Hot water in winter is an essential requirement for domestic uses such as bathing, cleaning of utensils and washing of clothes. In rural areas hot water is also required for softening of animal feed. Generally it is obtained by using firewood and cow dung cake in rural areas or using kerosene, liquid petroleum gas, coal or electricity in urban areas. Therefore, the use of solar water heaters will conserve lot of commercial and non-commercial fuels which are being wasted in merely getting hot water. A solar water heating system (SWHS) is made of several important elements: one or more solar collectors, a pump, a heat exchanger, a storage tank (or multiple tanks) and a back-up storage tank. The Solar heating system can be classified as passive or active. For water heating purposes, the general practice is to use flat plate solar energy collectors (FPC). The evacuated tube collectors (ETC) and evacuated tube heat pipe collectors (ETHP) are more efficient, though, the initial cost is comparatively higher. There are three type of water heaters extensively studied by various workers viz. natural circulation, collector-cum-storage and forced circulation in all over the world.

Introduction

A solar collector, the special energy exchanger, converts solar irradiation energy either to the thermal energy of the working fluid in solar thermal applications, or to the electric energy directly in Photovoltaic (PV) applications. For solar thermal applications, solar irradiation is absorbed by a solar collector as heat which is then transferred to its working fluid (air, water or oil). The heat carried by the working fluid can be used to either provide domestic hot water/heating, or to charge a thermal energy storage tank from which the heat can be drawn for use later (at night or cloudy days). Solar collectors are usually classified into two categories according to concentration ratios: non-concentrating collectors and concentrating collectors. A non-concentrating collector has the same intercepting area as its absorbing area, whilst a sun-tracking concentrating solar collector usually has concave reflecting surfaces to intercept and focus the solar irradiation to a much smaller receiving area, resulting in an increased heat flux so that the thermodynamic cycle can achieve higher Carnot efficiency when working under higher temperatures.

Introduction

Water is the primary source of life for mankind and one of the most basic necessities for crop production. The demand for water to irrigate the crops is increasing. For sustainable production from agricultural farms, irrigating the crops at right stages is highly important. Even in rainfed situation, lifesaving irrigation during long dry spell has also been found beneficial for crop survival and to obtain the targeted yield. Considering the depletion of groundwater below the critical zone in most part of the country, energy intensive pumping for irrigation is not a viable option. Therefore utilization of available runoff water through surface storage systems followed by pumping may be a potential solution to achieve the set goal of ‘crop per drop’ mission. In this connection, micro-irrigation system including drippers and sprinklers is of great importance. However, ensured power supply is essential to operate the micro-irrigation system even in remote areas. Reliable solar photovoltaic (PV) pumps are now emerging in the market and are rapidly becoming more attractive than the traditional power sources. These technologies, powered by renewable energy sources, are especially useful in remote locations where a steady fuel supply or electricity supply is problematic. About 16 million electric pumps and 7 million diesel pumps are in operations in the country for irrigation purpose; however they are highly energy intensive. Moreover, diesel operated and electrified pumps directly or indirectly emit large amount of CO₂ gas in atmosphere and hence are not environment friendly. To meet the energy demand for irrigation, solar PV pumps have been introduced under the off-grid power generation category of National Solar Mission (NSM) with a target of 1000 MW by the end of phase II (2013-2017). The target has been revised in 2015 to a total grid connected solar power generation of 1,00,000 MW comprising 40,000 MW roof top generation and 60,000 MW grid connected solar power plants (Resolution of MNRE, Govt of India, No. 30/80/2014-15/ NSM dated 1^st July 2015).

PV clad structures and hybrid devices for rural and agricultural applications have been developed after considering different design aspects, climatic parameters, solar radiation availability on different planes, heat transfer coefficients, performance of PV in arid region and inter phasing of different components while keeping in view, practicality, application and ease of operation. Performance of the developed systems was studied, mathematical model developed and energy savings worked out for techno-economic considerations. The chronology and details of the developed PV structures and hybrid devices are as follows:

Green structure for environmental control

First of all a Quonset type environment control structure having a length of 5.3 m and width 4 m was fabricated with angle iron and iron rods and it was covered by agro-net (75%) having surface area of about 55 m². The structure having a volume of 33 m³ was fixed with long side along east west direction and had provisions for incorporation of misting unit on west side and a door on east.

Introduction

For optimum use of harvested rain water in surface reservoir like farm ponds or tankas, small sized solar PV pumping systems with 1 hp AC and DC motor were experimentally tested at research farms of ICAR-Central Arid Zone Research Institute, Jodhpur (Fig. 16.1). Total suction head in both pumps was about 5 m. Among two installed pumps, the system with AC pump consisted of 1400 Wp (200 Wp × 7) whereas the DC pump consisted of PV array of 920 Wp (230Wp × 4). Each PV panel of AC solar pump was connected in series, which was further connected with an inverter to generate AC output of about 220-240 volt and 4-4.2 ampere. In case of DC solar pump, panels are connected in parallel to generate DC output of 40-50 volt and 15-20 ampere.

Introduction

Cultivation of crops in greenhouse or protected structure has been increased tremendously in recent times and also has been spread from temperate regions to the warmer regions of tropics and subtropics. Plentiful technologies have been developed for heating the inside environment of greenhouse for optimum plant growth (Sethi et al. 2013). However, in hot arid areas, cultivation in greenhouse is very difficult as the temperature reaches to a very high level to sustain plant growth (Pek and Hayles 2004, Saran et al. 2010). Under such situation requirement for cooling the greenhouse microclimate environment is the necessity, but is very energy and water intensive. Hence, in such regions, reduction of air temperature inside the greenhouse or the regulation of temperature closer to the ambient temperature during summer is necessary for successful crop production. A typical diurnal variation of temperature and global solar radiation during late winter months inside a shade net structure has been plotted in Fig. 18.1. It has been found that global solar radiation has been decreased to almost 1/3 of the radiation received outside by using 75% shade net cover. The temperature inside structure during morning time has also been found higher than outside temperature and in summer such increase is expected to be much higher.

Therefore, cooling is considered as the basic necessity for greenhouse crop production in tropical and subtropical regions to overcome the problems of high temperatures during summer months. Development of suitable cooling system that provides congenial microclimate for crop growth is a difficult task as the design is closely related to the local environmental conditions. Different cooling techniques for greenhouse crop cultivation may be found in Kumar et al. (2009). Fan pad cooling system has been widely used for greenhouse cultivation however, requires large amount of water during evaporation based cooling. Since water resources are becoming scarce in future such fan-pad cooling system may not be viable in future specifically in hot arid region.

Terrestrial or epiphytic members of the family Bromeliaceae that are grown in gardens for their unusually coloured and patterned leaves or showy inflorescences and fruits are referred to as ornamental ‘Bromeliads’. Being low growing, they are ideally displayed in containers or as epiphytes. They are sun loving, suckering in habit and have xerophytic adaptations. The leaves are tough and fibrous often with spiny margins and sheathing leaf bases that hold water. A grayish-white waxy or powdery coating often covers the lamina. Certain bromeliads have a water collecting depression at the center. Inflorescences are sunken, erect or reclining, with condensed or long and much branched stalks carrying colourful bracts and usually, less showy flowers. In many, the terminal leaves become brightly coloured at flowering. These and the floral bracts remain showy even after the flowers are shed.

Ornamental bromeliads popularly grown in gardens include the following:

Aechmea blanchetiana

Copper leaf bromeliad

Plants have strap like leaves that develop streaks of coppery red colour on the upper surface in bright sunlight.

Aechmea burle-marxii

Burle-Marx bromeliad

In soil culture, also known as in-ground culture, crops are raised on level ground as well as in raised beds. Soil culture is more often used in growing vegetables (e.g., tomatoes, lettuce, cucumbers, and peppers) rather than ornamental crops such as herbs, roses, freesia, and foliage plants as well as popular with smaller commercial and organic growers. The soil should be well drained, fertile, have a medium to light texture and be high in organic matter. The soil in a greenhouse should be worked as deeply as possible to break any existing hardpans or those that may have developed because of repeated traffic over the area. Fumigation or pasteurization of the soil is a must before each crop or at least once a year. This will help to destroy disease, nematodes and weeds. If soil is steamed, hold it at 180 degrees F (82°C) for 4 hours. Production techniques used in soil culture are similar to intensive crop production methods used in the field. Although soil is readily available at most places where a greenhouse is located most commercial growers, no longer rely on soil culture in the greenhouse as they did ealier. Some of the reasons include insect and disease pressures and costly fumigation.

There are different types of greenhouses, the low cost and high cost or low technology or high technology greenhouses. The cost of greenhouse and expenditure on crop production under these greenhouses would vary according to technology to be used and the crop is to be produced. The cost would vary from place to place with location of greenhouse. For each type of greenhouse its cost would vary according to geo-climate, the production practices and input expenditure/availability. The cost of construction of greenhouse in 2019 hence varies from Rupees 500 to 6000 per square meter.

4.1 INTRODUCTION

Ever since the man appeared on the earth, he has been harnessing the natural resources to meet his basic requirements. Reference to soil, water and air as basic resources, their management and means to keep them pure are mentioned in the Vedas, Upanishads and in ancient Hindu literature. Agriculture began sometime during 10000 BC and during 1930 AD use of chemical came into vogue (Sarswat et al., 2003). The phenomenal increase in population of both man and animal in the last century and fast growing industrialization and urbanization in last few decades have overstrained the natural resource base, which are getting degraded much faster than ever before. Thus, the attention of whole world is focused on how to increase production to feed the burgeoning population and the question uppermost in every ones mind is “how can we produce enough food without damage to the natural resource base, in a sustainable manner?” Precision farming is a promise in this regard. It appears to reduce risk of crop failure (De Boer, 1997).

8.1 INTRODUCTION

In developing countries like India, sustainable development of horticulture is a biggest challenge. There is need to formulate strategies for their management through “state of art” technologies for harnessing the naturally available resources in a sustainable manner to meet the ever growing demands of fruits. Canopy architecture has its role in influencing the productivity of fruits. It has been well highlighted by Halle and Olderman, (1970) and Halle et al. (1978). The emerging concerns in the area of canopy architecture and its management are the existence of large number of old senile orchards among the fruits crops and the development of a rejuvenation technology to restructure their canopy and enhance productive and remunerative plant life. Actually, canopy management “designing the plant as per need, using inherent plant characteristics in accordance with given set of conditions and resources to make the plant to perform maximum. As a result of canopy management, the trees acquire their natural shape which very often is not ideal for quality production. A majority of the trees attain tall, upright and curved growth structure and the canopy is marked with criss-cross branches leading to a highly dense vegetative mass with very poor penetration of active solar radiation. Such conditions not only affect the photosynthetic rate but also facilitate proliferation of pests as they prefer shady conditions. Consequently with pressure of pest abundance and poor photosynthetic efficiency over the years in want of due care of tree canopy architecture, the trees turn senile and unproductive and uneconomical.

Existence of such unproductive senile orchards has a telling impact on the socioeconomic as well as livelihood status of the farmers and consumers are equally affected with low poor production and poor quality of produce is the biggest problem with old orchard. Uprooting such orchards and undertaking new plantation may not be a wise option considering the long gestation period of orchard establishment and resulting impact on livelihood of farm families, environment health as well as the investment cost. Mass scale uprooting of trees for replacement of the orchard will also have very adverse impact on production scenario at the regional and national level.

17.1 INTRODUCTION

Flower consumption is growing at an alarming rate of 20 per cent per annum at global level and India is, today, one of the world’s largest consumer base and fastest growing retail destinations. Commercial floriculture is today a potential money-spinner and an economically viable agri-business.

Cut flower trade in terms of export oriented units dealing with rose and gerbera is flourishing. Other cut flowers like gladiolus, tuberose, lilum, chrysanthemum, etc. are also being cultivated along with traditional loose flowers like jasmine, rose, crossendra, marigold on a higher scale. However, growing awareness of Indian consumers and popularity for cut flowers is creating demand for diversified flower crops. Further, trade of some flowers in the market is responsible for gluts. Thus, there is an urgent need to diversify the floriculture industry by introducing and adoption of anthurium as a cut flower with unique shape and high longevity.

Precision farming is an advanced approach for the flower growers to enhance their income. It mainly includes information and technology of farm management system to identify, analyze and manage variability within fields for optimum profitability, sustainability and protection of the land resource. Precision farming is applied for increasing efficiencies that can be realized by understanding and dealing with the natural variability found within a field.

Anthurium is taking a good place in market with high value. Farmers are undertaking anthurium cultivation and in this direction, precise farming technology will help the farmers to grow the crop with good quality and higher production per unit area.

19.1 INTRODUCTION

Indiscriminate use of agro-chemicals over the last five decades has adversely affected soil fertility, crop productivity, produce quality and particularly the environment. These degenerative effects of intensive agriculture have compelled to think for alternative and sustainable system of agriculture. As a result, number of alternative systems viz Biodynamic, Rishi Krishi, Panchagavya Krishi, Natueco Organic Farming, Homa Organic Farming, Natural Farming and Jaivik Krishi have emerged in different parts of the country. In organic farming production is achieved without use of chemical input (Singh, 2011 and Malik, 2000). In these systems, maximum reliance is placed on self-regulatory agro systems and locally available or farm derived renewable resources. Since the soil, water and environment have been contaminated, hence even organic production techniques once effective are now are not capable of mitigating these issues. This has created number of myths in the mind of policy makers and even large number of farmers.

26.1 INTRODUCTION

India is the second largest producer of fruits and vegetables and ranks next to Brazil and China, respectively. With the commensurate total production of 63.5 and 87.2 million tonnes of fruits and vegetables, the country shares about 11 per cent of total production under fruits and 13 per cent of total production under vegetables, respectively (Anon., 2008) in global scenario. Horticultural produce undergoes spoilage at the time of harvesting, handling, storage, marketing and processing resulting in huge wastage. Efficient management of this waste can help preserving essential nutrients of our food and feeds and bringing down the production cost of processed product, besides minimizing pollution hazards and purifying the environmental condition. Recycling of horticultural waste is one of the most important aspects of utilizing it in a number of new ways to yield new products and meeting the requirements of essential products required to mankind.

In advance countries like USA, Australia, Brazil and Israel, more than 50 per cent of fruits and vegetables productions are processed into different products, whereas, in India it is less than 2.0 per cent of total production. Generally, post harvest losses in fruits and vegetables range between 25-30 per cent of total production, valued at more than 57,000 crores annually due to lack of availability of appropriate post harvest infrastructure (Guleria and Verma, 2008). Horticultural crops are grown all over the country incurring wastes everywhere. There are different stages of waste from farm to fork.

28.1 INTRODUCTION

Precision Farming has assumed great significance in the country for making the use of available resources more judiciously and efficiently. It is farm management strategy which utilizes precise information to increase profit and to reduce enviornmental impact. It takes into account in field variability in soil fertility and crop conditions. In nutshell, it may be defined as management of input variables such as application rates, cultivars selections, tillage practices, irrigation scheduling, optimization of soil and plant nutrient through nutrient management practices and management of pest and diseases by reducing the quantities of pesticides. Development of new technologies has become integral part of precision farming.

Precision farming was widely accepted firstly as a management practice in corn belt of mid west United States of America which helped in increasing the yield and significant reduction in production costs. Besides, crops like wheat and soybean have also been included in its purview. In south east parts of U.S.A., research is currently on in the crops of citrus, cotton, groundnut, vegetables and even livestock. Precision farming management practices have also integrated controlling and monitoring of field information using geographical information system (GIS), Global Positioning System (GPS), sensors, Variable Rate Technology (VRT) and yield monitoring. These components are highly sophisticated. GIS software stores data pertaining to soil type, nutrient levels etc in layers and assign that information to the particular field location. It can also be used to analyze characteristics between layers to develop application maps or other management options. Global Positioning System is a referencing device capable of identifying sites within a field. Global positioning system (GPS) helps in storing information regarding field location in terms of latitude and longitude. Maps showing variability of nutrient levels soil types, topography, pest incidence and yield can be created by virtue of it. It is a set of 24 satellites in high altitude orbit above the earth for pin pointing objects on the surface of the earth. These satellites continuously transmit radio signals which are picked up and deciphered by special receivers. A GPS receiver requires at least four satellites to determine its position on earth. Receivers are mounted on tractors/top of cab or on some high point to get a clear view of the spy. Crop stress, soil properties, pest incidence etc. are determined by the help of sensors through traveling over the field by tractors or combine. These can be used for measurement of soil conductivity, fertility status, weeds etc. Another type of sensor viz Remote sensors (aerial/ satellite) can provide instead maps of field conditions. Computer control device and associated hardware are part of Variable Rate Technology (VRT) for application of fertilizers, pesticides etc on the basis of field characteristics of the specific location. In precision farming, information is needed about mean characteristics of small, relatively homogenous management zones. It is data acquisition of the farms to find the soil, vegetation and other parameters which are amenable to remote sensing. Remote sensing techniques are helpful in providing continuous acquired data of agricultural crops. Remote sensors image vegetation growing on different soil types with different water availability. The following applications can be made by relief sensors:

4.1 Selection of Isotopes for Tracer Studies

In radioisotopes we have all the essentials of a powerful research tool, a non-destructive means by which atoms could be followed and detected in the organism. Application mainly depends on the fact that chemical properties of the isotope of an element are essentially identical.

The basic requirement of a tracer is that it be

1.Chemically and physically, virtually equivalent to the substance it represents or displaces and that it, in no appreciable way, affect the system differing from its normal counterpart.

2.Radioactivity could be detected directly and

3.Unaffected by the physical or chemical treatments that are given. Hence, this has been used widely in the investigations of the various problems in biology, chemistry, physics and other fields like agriculture.

Although a number of radioactive isotopes are found in nature, most of the isotopes used as radioactive tracers are produced artificially. Methods for producing radioactive isotopes include nuclear fission and the bombardment of selected materials by neutrons or positively charged particles.

6.1 Production of Radioactive Nuclides

Nuclear Reactors

In a nuclear reactor, a fissionable nuclear fuel such as ²³⁵U or ²³⁹Pu is placed in a defined geometry within the reactor core. Fast moving neutrons released as the ²³⁵U or ²³⁹Pu nuclei fission are slowed to thermal energy (0.025 eV) by a low-Z moderator such as water, heavy water, beryllium, or graphite surrounding or mixed intimately with the nuclear fuel. The slowly moving neutrons are absorbed by addition of ²³⁵U or ²³⁹Pu nuclei, and a chain reaction is maintained. The rate of absorption of slow neutrons by fissionable nuclei determines the fission rate for the reactor and is controlled by placing inert neutron absorbers in the reactor core. Typical neutron absorbers, usually termed control roads include boron and calcium. Heat generated in the reactor is removed by a coolant such as water or one of a variety of gases or liquid materials.

7.1 Measurement of Radiation Dose

Radiation dosimetry involves the measurement of the energy actually absorbed in a substrate exposed to ionizing radiation. The radiation may be either electromagnetic or corpuscular in nature. The passage of electromagnetic radiation through a substrate causes ionization by virtue of the secondary electrons released. Particle radiations on the other hand, ionize directly because they are charged, or indirectly through the charged particle resulting from a collision with a substrate. Alpha, beta particles, and protons yield primary ionization. Gamma rays and neutrons produce ionizing radiation by their interaction with the substrate in passage through it.

Mesenchymal stem cells are multipotential cells capable of proliferation and differentiation into osteogenic, chondrogenic and adipogenic lineages both in vitro and in vivo. Minimal criteria for defining MSC according to International Society of Cell Therapy (ISCT) are (a) they must be plastic-adherent when maintained in a standard culture conditions, (b) MSC must express some surface antigen such as CD105, CD73, CD90, CD44 and must not express CD34, CD45, CD 14, CD19, and HLA-DR, and (c) MSC must differentiate in vitro into osteoblast, adipocytes and chondrocytes under specific differentiating condition.

Although traditionally MSC isolated from bone marrow, more recent reports have detailed the isolation of cells with MSC characteristics from a variety of tissues including umbilical cord blood, chorionic villi of the placenta, Wharton’s jelly, peripheral blood, fetal liver and lung, adipose tissue, skeletal muscle, periosteum, deciduous teeth, amniotic fluid, dental pulp, testis, synovium and the circulatory system. Bone marrow and periosteum sources are richest in young animals with their numbers diminishing, but still present in old age. It is estimated that MSCs represent approximately only 0.01 to 0.001% of the total nucleated cells within isolated bone marrow aspirate. The yield of MSCs from bone marrow varies from species to species. e.g., Canine =1 in 2.5 × 10⁴; Feline =1 in 3.8 × 10⁵; Murine =1 in 10.8 ×10³ to 1 in 3.45×10⁴ and Horses =1 in 4.2 × 10³. Despite this low number, they are remains a great interest, as they can be easily isolated from a small aspirate and culture-expanded through as many as 40 population doublings to significant numbers in about 8 to 10 weeks.

Mesenchymal stem cells are multipotential cells capable of proliferation and differentiation into chondrogenic, osteogenic and adipogenic lineages. Minimal criteria for defining MSC according to International Society of Cell Therapy (ISCT) are (a) they must be plastic-adherent when maintained in standard culture conditions, (b) MSC must express some surface antigens such asCD105, CD73, CD90, CD44 and must not express CD34, CD45, CD 14, CD19, and HLA-DR, and (c) MSC must differentiate in vitro into osteoblast, adipocytes and chondrocytes under specific differentiating condition. 
Although MSC is traditionally isolated from bone marrow, more recent reports have detailed the isolation of cells with MSC characteristics from a variety of tissues including umbilical cord blood, chorionic villi of the placenta, Wharton’s jelly, peripheral blood, fetal liver and lung, adipose tissue, skeletal muscle, periosteum, deciduous teeth, amniotic fluid and synovium. Bone marrow and periosteum sources are richest in young animals with their numbers diminishing, but still present in old age. One of the attractive advantage of bone marrow derived mesenchymal stem cells (BM-MSCs) as a source of cell transplantation is their low immunogenecity. Recently, several studies have reported that BM-MSCs may be immune-privileged cells that do not elicit immune response due to an absence of their immunologically relevant cell surface markers. BM-MSCs also are known to inhibit proliferation of T lymphocytes, B lymphocytes, dendritic cells and natural killer cells.