eChapters

Aim

To synthesize silver (Ag) nanoparticle.

Introduction

The Ag nanoparticles easily interact with other particles and increases their antibacterial efficiency. The Ag nanoparticles can be synthesized using various methods: biological, chemical electrochemical, γ-radiation, photochemical, laser ablation etc (Udapudi et al., 2012). The most popular preparation of Ag colloids is chemical reduction of Ag salts by sodium borohydride or sodium citrate (Udapudi et al., 2012).

Most of the bones of the skull are joined (articulated) with the adjacent bones by sutures. These are immovable joints (synarthroses). The uniting medium is a fibrous tissue and is called the sutural ligament. Most of these joints exist in the early life of the animals and as the age of the animal advances the adjacent bones fuse with each other.

1. Botanical Name : Securina leucopyrus syn. Flueggea leucopyrus

2. Family : Euphorbiaceae.

3. Common Name : Bushweed, Indian snow berry, White honey shrub

4. Name in Indian languages

Katupila, Shinar (Hindi), Pandharphali (Marathi), Mulluppulatti (Tamil), Cerimklaav (Malayalam), Pulugudu (Telugu), Bilchuli (Kannada), Parpo (Konkani), Humri (Gujarati), Shwetakambuja(Sanskrit), More pan (Kodwa).

5. Description

Bush weed is an erect shrub of family Euphorbiaceae. The plant grows up to 4 m tall with branches cylindrical or obtusely angular. Leaf is elliptic, obovate, or round papery to thinly leathery. Flower cymes arise in leaf axils or at leafless nodes. Flowers are tiny and yellowish. Fruit is a nearly spherical berry, about 4 mm in diameter, whitish when ripe.

Transformation

The uptake of DNA by competent cells is called transformation. Transformation involves:

Mix a small volume of DNA (usually 1-3 µl) with 100 µl of competent cells.

The mixture is incubated on ice for 30 minutes to allow the cells to take up the DNA.

The cells are then heat-shocked at 37 to 42°C for 1 minute, and cold shocked on ice for 2 minutes.

The heat shock/cold shock reverses the effect of the competent cell process, by healing the damage inflicted by the competent cell solution.

Add 0.5 ml of LB culture mediumz

Incubate the cells for 10 - 30 minutes at 37°C to aid the healing process.

This cell/medium mixture is then spread onto bacterial culture plates containing agar, LB, and a selection mechanism.

Failure in timely diagnosis of diseases and other pests has often been responsible for devastating losses. Reducing crop losses by keeping pests at bay is crucial to food security. Plant clinic is an innovative paradigm which plays a vital role in assuring food security and ushering prosperity by providing timely diagnosis and rendering necessary advice to the growers, gardeners and other stakeholders for managing pest problem in India. Plant clinics are all about plant health. Though the major role of plant clinic lies in diagnostics and advisory, the activities of plant clinic extend beyond plant clinic, with emphasis on extension, working more closely with farmers and organizations involved in promoting food production. The role of plant clinics beyond diagnostics and advisory. These are:

Radio script should be prepared in such a way that listener should feel that somebody is talking with him in a friendly manner and with informal style. For this purpose:
1. Collect the material from your experience.
2. Keep the purpose of the script and audience in mind.
3. Decide the method of presentation of information.
4. Considering the time available for the talk, the script should be prepared.
5. Generally we talk two words per seconds and 8 to 10 lines are required per minute having 5-6 words per sentences.
6. Time for talk is always limited hence select one idea at a time never include maximum technical information in the script.
7. Arrange the information in the sequences.
8. Include supporting and illustrated facts.
 

1. Introduction
All glassware used for bacteriological work must be meticulously cleaned and sterilized. Used and soiled glassware should be thoroughly washed to remove dirt and solid residues before drying and undergoing sterilization.
2. Cleaning of New Glassware
Borosilicate glass (e.g., Pyrex) and factory-washed soda glass require only standard washing before use. However, new, unwashed soda glass needs additional treatment to neutralize alkali content.
• Place the items (e.g., test tubes, Petri dishes, and flasks) in a suitable container, cover with a 1% tri-sodium phosphate solution, and bring to a boil.
• Remove the items, rinse with tap water, and immerse them in a 1% hydrochloric acid solution to neutralize alkalinity.
• Rinse thoroughly with tap water, followed by distilled water. Allow the glassware to dry before sterilization.
3. Cleaning of Used Glassware and Other Apparatus
• Autoclave all glassware contaminated by microorganisms (121°C for 15–20 minutes). This liquefies solid media, facilitating removal.
• Rinse the empty glassware with tap water, then warm water (50–55°C).
• Soak in a detergent solution (e.g., 1% washing soda or tri-sodium phosphate solution) and scrub with a brush.
• Rinse thoroughly with tap water to remove detergent traces, followed by distilled water.

    1.    Molar tooth cutter :  It is used to cut the extra sharp molar tooth in large animals (Fig. 7.1).

Introduction

Hydrogels are loosely cross-linked, highly hydrophilic, organic polymers. They can absorb and retain aqueous fluids up to 500 times of their own weight (Singh et al., 2011). In the field application, such absorbents exhibited a limited swelling capacity within the soil matrix.

1. Botanical Name : Tamilnadia uliginosa (Retz.) Tirveng. & Sastre

2. Family : Rubiaceae

3. Common Name : Divine Jasmine, Tamilnadia

4. Name in Indian languages

Kalaphendra (Marathi), Wagatta, Kalikarai (Tamil), Pindichakka (Malayalam), Kuka-elka (Telugu), Pidaar(Nepali).

5. Description

Divine jasmine is a very rigid, ramous, small tree, armed with numerous strong thorns. This found in bogs, swamps, banks of rivers and other moist places. It flowers in the beginning of the hot season. The flowers of this species, are known for beauty. It is good shrub for flower garden. It is an important medicinal plant because each and every part of it can be used for medicinal purposes. Sometimes leaves of the plant are used to make chutney.

6. Origin and distribution

It is native of India. It is distributed in India, Bangladesh, Srilanka, Thailand, Vietnam. In India, this species is found in moist deciduous forests.

TV Talk
The talk delivered through TV i.e. through an electronic audio-visual medium which provides pictures with synchronized sound. The medium is cosmopolite in nature and can be used to develop instant mass awareness.

Important points while writing a programme for broadcasting
• Writing should be catchy, readable, conversational sound, understood and absorbed by the audience.
• Writing should be in simple, known language avoiding too much technicalities
• Interest of the listener should be sustained continuously
• Application of figures should be done judiciously
 

Catheters are hollow tubes made up of rubber, gum elastic, polyethylene or teflon or metal and are indicated.
 
    1.    To evacuate fluid from the body cavity.
 
    2.    To collect fluid sample from the hollow organ.
 
    3.    To administer medication inside a hollow organ.

(Method of Saryre, 1963 and IAEA, 1969)
Introduction

This technique helps in detection of very small amount of stable elements in fairly large quantitites of extraneous matter. Trace elements like zinc, copper, molybdenum and arsenic which exist in minute amounts in certain organs or tissues. For example, to detect the amount of copper present in one gram of tissue. using 1 mg of thin copper foil as control. Normal copper contains 69 percent ⁶³Cu and 31 percent ⁶⁵Cu. The (n,r) reactions on these two stable isotope produce ⁶⁴Cu and ⁶⁶Cu respectively. The half life of ⁶⁴Cu is 12.8 h and that of ⁶⁶Cu is five minutes. Therefore the latter can be ignored, if more than that an hour elapses between the end of the irradiation and the measurement. The specific activity of the unknown separate is compared with that of the known standard separate, and the content of Cu in the original tissue sample is thereby calculated.

Principle

The principle of Electrophoresis involves the motion of a particle in a fluid medium, where the gravitational attraction (on a flowing liquid) is the sedimenting force, and the frictional force is the opposing force and is proportional to the velocity of the particle. These forces balance each other acting in opposite directions and hence balance each other. This will lead to the uniform motion of the particle in the mobile liquid medium (i.e. the particle moves at constant velocity). In case the external field is an electric field instead of a gravitational field, a macromolecule will respond to the external field in two ways. In case the molecule is charged, it will migrate in an electric field to the electrode of opposite charge. Under this principle the technique of electrophoresis functions.

All electrophoresis operations are governed by the principle:

The essential plant nutrients, or essential elements, are listed in the Table 41. It enumerates 16 chemical elements of which the essentiality for plant growth and reproduction has been established according to the criteria formulated by D.I. Arnon and P.R. Stout, 1939. This listing has existed since 1954 with chlorine as the latest addition (cited by Hopkins 1999).

These plant nutrients are classified into various groups:

1.    Major elements and trace elements : Of the 16 listed, 9 are major elements or macronutrients and 7 are trace elements or micronutrients. Major elements are those which are needed by plants for their growth and reproduction in relatively large amounts while trace elements are needed in smaller amounts compared to the former. The major elements are further sub classified into structural elements (carbon, oxygen and hydrogen), primary macronutrients (nitrogen, phosphorus and potassium), and secondary macronutrients (calcium, sulfur, and magnesium). Micronutrients are Zn, Cu, Mn, Fe, Mo4 and boron (Table 41).

1. Botanical Name : Vitis himalayana Br.

2. Family : Vitaceae

3. Common Name : Wild grape, Himalayan Woodbine

4. Name in Indian languages: Bhambti, phlankur (Himachali), Chappar tang, Bara churcheri (Kumaoni).

5. Description

This climbing shrub is commonly found in the forests. One of the important characteristics of this wild grape is that they ripen very late i.e., in October. The plant has tuberous roots.

6. Origin and distribution

Himalayan Woodbine is found in the Himalayas, from Pakistan to Sikkim, South West China and Myanmar at altitudes of 1800-3300 m. In India, it is found in Himachal and Uttarakhand.

7. Morphology

Himalayan Woodbine is a very large climber, growing to 18 m long. It has trifoliate leaves with 3 ovate long-pointed, sharply toothed, stalked leaflets. Leaves are shining green above and pale on the underside, about 10 cm long. The lateral leaflets have asymmetrical bases. Flowers are tiny, yellow-green, in spreading flat-topped clusters. Flowers have petals about 5 mm long, with 4-5 stamens. Fruit is a black berry.

Recommendations

Based on the empirical research, following recommendations can be made for this specific area of research:

(i) Land size based planning for business communication can help the growth and success of farm enterprise.

(ii) Rice based farming system, supported by irrigation is offering a unique opportunity for capacity building of the farmers of West Bengal.

(iii) Training, focusing on business communication, handling ICT tools and mobile telephony, can go a long way for making communication behavior more adaptive to present social ecology of agriculture and allied sectors of West Bengal.

(iv) Social capitals like, Schools, Panchayat, Credit organizations, Market linked institutions, are to be integrated for shaping up a new form of Entrepreneurial communication behavior.

Limitations: The most important limitations which have been observed in this research are as follows:

Present work was undertaken to study the effect of doxycycline and doramectin inmale rats. Following exposure to doxycycline hyclate orally for 28 days at dose rate of 10,16.50 and 33 mg/kg, rats of any doxycycline treated groups did not show any clinicalsigns, mortality or abnormal behaviour. Percent body weight gain was significantlyreduced in rats of group treated with doxycycline (16.50 and 33 mg/kg). A significantincrease in relative weight of left and right testes was found in rats exposed to doxycycline(33 mg/kg). Doxycycline did not produce any change in haematological parameters in ratsof doxycycline treated groups. Blood glucose level, total cholesterol, LDL cholesterol andBUN levels was significantly increased in rats of group exposed to doxycycline (33mg/kg). Rats of group treated with doxycycline (16.50 mg/ kg) exhibited a significantdecrease in blood HDL cholesterol level. Hepatic SOD level was significantly increased inrats of group treated with doxycycline (33 mg/kg).

Apricot is commonly known as Armenian plum. Its scientific name Prunus armeniaca derives from the assumption that apricot was known in Armenia during ancient times and has been cultivated there for so long that it is often thought to be native of that place. The cultivated apricot has its origin in North-Eastern China, whereas wild apricot, popularly known as ‘Zardalu’, appears to be indigenous to India. Generally, there are two types of apricot, namely, sweet kernel type and bitter kernel type.