eChapters

Introduction Antimicrobial resistance (AMR) has emerged as one of the gravest threats to modern medicine, undermining decades of progress in infectious disease control. It is cited by the World Health Organization (WHO) as one of the top 10 threats to global health. In 2019 alone, AMR was associated with approximately 4.95 million deaths, including 1.27 million directly attributable to resistant bacterial infections. This makes AMR a leading cause of death worldwide, surpassing HIV/AIDS (0.86 million deaths) and malaria (0.64 million deaths) in the same year. Projections suggest that if the trend continues unchecked, AMR could cause 10 million deaths annually by 2050, representing a profound human-cost escalation. Livestock and Animal burden While the quantitative burden of AMR in animals is not yet globally mapped, its effects in livestock are already both profound and alarming. Over 70% of all antimicrobials globally are used in farm animalsfrequently for growth promotion and disease prevention—not just treatment. This widespread use contributes to emerging resistance in key pathogens—Salmonella, E. coli, Campylobacter in poultry, and severe swine dysentery in pigs. In some outbreaks, swine dysentery, resistant to conventional treatment, has caused up to 90% morbidity and 30% mortality in weaned pigs. Animal-to-Human Transmission and the One Health Paradigm Antimicrobial resistance in livestock doesn’t stay on the farm—it seeps into the environment and crosses over to humans via food chains, direct contact, and environmental pathways. For example, substandard veterinary medicines and antibiotic misuse has been traced in zoonotic infections and veterinary-tohuman AMR transmission.Surveillance gaps remain: the World Organisation for Animal Health (WOAH) acknowledges that while human-related AMR mortality is well-documented, the animal health burden is not yet quantified globally—though numerous projects are underway to address this gap. The failure of conventional antibiotics to treat resistant infections highlights the urgent need for alternative therapeutic strategies. Among the most promising options is bacteriophage therapy—the use of viruses that specifically target and kill bacteria. Bacteriophages Bacteriophages, often referred to simply as phages, are viruses that infect and replicate within the bacteria. They are considered as the most abundant biological entities on Earth, with an estimated amount 10³¹ phage particles present in the biosphere—outnumbering bacteria by approximately tenfold. Phages are naturally found in diverse ecosystems including soil, ocean, sewage, and even within the microbiomes of humans and animals.

Bacteriophage is the term applied to bacterial viruses. Bacteriophage having the ability to lyse certain sensitive living cells. Phages are small particles, which can only be seen in the electron microscope after the application of suitable staining techniques. All phages have a head and most have a tail. The head may be isometric or prelate with a hexagonal outline.

There are two types of phages virulent and temperate which enter a different relationship with the host, the lytic and lysogenic cycles respectively.

Bacteroides spp are non-spore forming gram-negative bacilli that are part of the human resident flora. Microbiologically, they are distinguished from other genera by growth in 20% bile. At present, the Bacteroides fragilis group consists of ten species: B. fragilis (the most frequent isolate), B. distasonis, B. thetaiotaomicron, B. vulgatus, B. ovatus, B. eggerrthii, B. merdae, B. stercoris, B. uniformis, and B. caccae. Since 1990, many organisms previously designated as Bacteroides have been reclassified (see chapter on Anaerobes other than Bacteroides). Bacteroides, the predominant genus in the human
intestine, are important in numerous metabolic activities and may provide some level of protection from invasive pathogens. All 10 species are usually isolated from the colon, although infections caused by or associated with them can include virtually any organ.
Characteristics
The gram-negative Bacteroides spp. or closely related genera are capsulated obligatory anaerobic bacilli that are non-spore forming, pale-staining, and some are motile by peritrichous flagella, while other taxa are non-motile. Bacteroides, Parabacteroides, Odoribacter are generally bile resistant, distinguished from genera which are bile sensitive. They are normally commensal, found in the
intestinal tract of humans (mouth, colon, urogenital tract) and other animals. Many cultures of Bacteroides strains display brown to black pigmentation on blood agar media caused by esculin hydrolysis.
Morphology
In general, all strains were stained poorly with safranin, but well with dilute carbolfuchsin. Motility was not observed in any strains. The morphology of Bacteroides fragilis in fresh cultures and in pus is not
especially characteristic. In this form, the bacillus measures 0.5 by 2.0 to 2.5 micra. Older cultures tend to develop larger forms (0.6 to 0.8 by 2.0 to 9.0 micra) but even in this state the organism cannot be distinguished from aerobic Gram-negative bacilli, by its morphology.

12.1 Bactofugation
Using a specially constructed hermetic centrifuge, the Bactofugation® method separates bacteria—including especially the spores produced by particular bacterial strains—from milk. The RPM rises to 60000. Since their density is larger than that of milk, this technique has shown to be a quick approach of lowering the spore count in milk. Usually separating the milk into a portion more or less free from germs, bactofugation produces a concentrate (bactofugate), containing generally both spores and bacteria in general and amounts to up to 3% of the feed to the Bactofuge.
12.2 Dehydration
One can preserve the milk by dehydrating to different degrees. The “shelflife” of the dehydrated milk is longer than that of milk kept refrigerated and pasteurised. Concentrated or dry milk (milk powder) is milk from which some or all of the water has been removed. More than eighty-percent of milk is water. Eliminating water can help to preserve milks the best way. One can accomplish it freeze drying or by drum or spray drying.
12.2.1 Condensed Milk
Eliminating some of the water from whole or partially/fully skimmed milk, either with or without sugar, results in condensed milk. Generally speaking, “condensed milk” is “full cream sweetened condensed milk,” whereas “evaporated milk” is used for “full cream unsweetened condensed milk.” Variations in skimmed milk are labeled “sweetened condensed skim milk” and “unsweetened condensed skim milk.” For full cream products, milk solids have a concentration ratio of about 1:2.5; for sweetened condensed skim milk, that ratio is 1:3. Annexure-I has an exhaustive composition chart for condensed milk.

Bael is one of the oldest and useful trees of India. The bael fruit, (Aegle marmelos Correa) is also called Bengal quince (John and Stevenson, 1979), Indian quince, golden apple, holy fruit, stone apple, bel, belwa, sriphal and maredoo in India. Among the indigenous fruits of India, Bael occupies an important place. All the parts of bael tree have their own importance. It is a medicinally and economically important fruit crop with immense potential for extending its cultivation in semi-arid degraded land. Mention of bael cultivation in Indian literature dates back to 800 B.C.  Bael fruit is available in almost all states of India but most abundantly available in Uttar Pradesh, West Bengal, Bihar and Orissa.

The Bael fruit is one of the most nutritious fruits. It contains 61.5 g water, 1.8 g protein, 0.39 g fat, 1.7 g mineral, 31.8 g carbohydrate, 55 mg carotene, 0.13 mg thiamin, 1.19 mg riboflavin, 1.1 mg niacin and 8 mg vitamin C / 100 gm of edible portion (Gopalan et al., 1971). It contains the highest riboflavin content among fruits. Marmelosin is, most probably, the therapeutically active principle of bael fruit. It is isolated as a colorless crystaline compound (Dixit and Dutt., 1932).The astringent taste of bael fruit is due to phenolic compounds. The medicinal properties of bael have been dealt with in Charaka Samhita (Aiyar, 1956). Bael fruit has curative, pesticidal and nutritive properties. The leaves of bael are used for worship of Lord Shiva. Green bael fruits are used for preparing Murabba (preserve) generally taken for stomach ailments. Bael fruits have an immense potential for processing into products like squash, jam, toffee, candy and syrups for internal and foreign market.

Bael (Aegle marmelos Correa.) also known as Shri Phal, Baelpatra, Bengal quince, Beel, Bel is a nutritious and medicinal fruit plant, which is most suitable for water scarce areas of the country. It is indigenous tree to India and known since ancient time. Bael fruit is mentioned in ancient literature viz., Vedas, Ramayana, Upavan Vinod,  Brihat Samhita (Singh and Roy, 1984). Methods of ripening of the bael fruit are also given in Buddhist and Jain literature 800 BC – 325 BC.  During the Ramayana period, it has been mentioned that bael trees were found growing in the Chitrakut hills and Panchvati. The bael is grown throughout the country and main bael growing states are Uttar Pradesh, Bihar, Madhya Pradesh, West Bengal, Odisha and Rajasthan. It is found growing in neighboring countries like Nepal, Sri Lanka, Pakistan, Bangladesh, Myanmar, Thailand and most of the Southeast Asian countries. Besides systematic orchard, bael can also be grown in parks, temple gardens, roadsides etc. The bael plant is sacred to the Hindus and is of common occurrence in temples of ‘Lord Shiva’ because of its mythological significance.

Bael (Aegle marmelos, L.) belongs to the family Rutaceae and is an important indigenous fruit of India known since 2000-820 BC. Bael is considered to be native to India and is grown all over the country. It is also seen in south east Asian countries like Sri Lanka, Pakistan, Bangladesh, Burma, Indonesia and Thailand. It is a hardy plant and can be grown even on marginal soils.

The leaves are trifoliate and aromatic. The leaves of the tree are used in religious offerings. The fruit is usually globose, grey or yellowish with small hard aromatic rind. The pulp is thick mucilaginous, orange yellow in colour with sweet scented aroma. The importance of bael fruit lies in its curative properties. It is used in Ayurveda for its medicinal properties.

There are no recognized or improved varieties of bael with a standard name. It usually goes with the name of locality or region it is grown in. Ayodhya, Etawah, Faizabad Local, Gonda Selection, Kagzi Bael, Kagzi Kalan, Mirzapuri, Darogaji, Ojha, Rampuri Azamati, Khamaria, Deoria Large, Gonda-1 and NB-5 are a few to name.

Uses

Bael fruits cut in half, or the soft types broken open, and the pulp, dressed with palm sugar, eaten for breakfast, as is a common practice in Indonesia. The pulp is often processed as nectar or “squash” (diluted nectar). A popular drink (called “sherbet” in India) is made by beating the seeded pulp together with milk and sugar. A beverage is also made by combining bael fruit pulp with that of tamarind. These drinks are consumed perhaps less as food or refreshment than for their medicinal effects.

Mature but still unripe fruits are made into jam, with the addition of citric acid. The pulp is also converted into marmalade or sirup, likewise for both food and therapeutic use, the marmalade being eaten at breakfast by those convalescing from diarrhea and dysentery. A firm jelly is made from the pulp alone, or, better still, combined with guava to modify the astringent flavor. The pulp is also pickled. The young leaves and shoots are eaten as a vegetable in Thailand and used to season food in Indonesia. They are said to reduce the appetite. An infusion of the flowers is a cooling drink. (Morton, 1987).

Introduction

Bael (Aegle marmelos Corr.) is an indigenous fruit of India belongs to family Rutaceae and it is commonly known as Bengal quince (John and Stevenson, 1979), Bilva, Indian quince, Golden apple, Holy fruit, Bel, Belwa, sriphal, bilipatra Stone apple and Maredo in India. The deciduous with trifoliate aromatic leaves is traditionally used as sacred offering to the ‘Lord Shiva’ and thus the tree is also known by the name Shivaduma (The tree of Shiva). It has tolerance to arid conditions as well as high rainfall. It is known from prehistoric time and mentioned its importance in different ancient and medicinal system viz., Rigveda, Yajurveda, Atharvaveda, Charak Samhita and Sushrut Samhita, and Ayurveda, Siddha, Unani medicines and religious Jain and Buddhist literature (Balunas and Kinghorn, 2005, Om Prakash,1961 and Kirtikar and Basu, 1935). It can be grown successfully in water scarce and rainfed areas of the country. Besides, occurring as natural wild plant of forest, it is commonly grown in the homestead gardens, backyards, and religious places particularly in the premises of the Lord Shiva temples and in the farmers’ fields. In India, bael is being grown throughout the country except temperate region, but there is no systematic plantation of bael except Uttar Pradesh, Rajasthan and Gujarat where few progressive farmers have initiated growing bael economically (Dhankhar et al., 2011). Its fruit is highly nutritive and rich in vitamins, bioactive compounds, minerals, carbohydrate, etc (Gopalan et al., 1964). Every part of the tree such as fruit root, bark, leaf and flower of any stage of maturity and ripening are important ingredients of several traditional formulations. Recently, advanced scientific techniques have reported that plant has various medicinal properties viz, anticancer, antibacterial, antifungal, antidiabetic, antioxidant, hepatoprotective, haemolytic, larvicidal and anti-inflammatory activities. The sherbet and marmalade prepared from fruit pulp is prescribed for diarrhea and dysentery. It is said that the ripe fruit is a tonic, an astringent and laxative, whereas unripe fruit is an astringent and digestive and good for heart and brain. The important medicinal properties of bael are antidiabetic, antimicrobial, anti-inflammatory, antipyretic, analgesic, cardioprotective, antispermogenic, anticancer and radio protective (Kumar et al., 2010 and Vadivelan et al., 2011) The fruit is efficient to avoid malnutrition and health insecurity in the underprivileged. The ripen and unripe fruits can be used for preparation of various value added products such as jam, slab, squash, powder, candy, nectar, toffee, pickle, wine, sherbet, ice cream etc.

Bael is commonly known as Bengal quince, Indian quince, golden apple, holy fruit, stone apple and bel. It is more prized for its medicinal virtues than its edible quality.  The deciduous tree with trifoliate aromatic leaves is traditionally used as sacred offering to ‘Lord Shiva’. Fruit is a hard-shelled berry and very well-known for its medicinal properties due to marmelosin content. The ripe fruit is tonic, restorative, laxative and good for heart and brain. The pulp is often processed as nectar or squash. A popular drink called “Sherbet” in India is made by beating the seeded pulp together with milk and sugar. A beverage is also made by combining bael fruit pulp with that of tamarind. These drinks are consumed perhaps less as food or refreshment than for their medicinal effects. For medicinal use, the young fruits, while still tender, are commonly sliced horizontally and sun-dried and sold in local markets. The fruit, roots and leaves have antibiotic activity. The root, leaves and bark are used in treating snakebites.

Genus : Aegle 
Species : marmelos (L) Correa
Family : Rutaceae
Chromosome No. : 2n = 18(36)

Bael (Aegle marmelos Correa) besides being indigenous to N. India it is also one of the most useful medicinal plant of India, as it has number of curative properties. In Ayurveda the Indian medicine system use of unripe fruit is advised for acute dysentery and diarrhoea whereas ripe fruit acts as an effective laxative. Amongst the fruits native of India, bael is said to be the most nutritious fruit, is rich in protein minerals, carbohydrates, vitamin C as well as in riboflavin content. Despite these quality statistics the fruit failed to become popular (as dessert) primarily it being very seedy with thick hard shell and mucilaginous flesh but the processing qualities needs to be exploited judiciously.

• No other fruit has such a high content of Riboflavin.
• The Bael is a native of the Indo-Malayan region and has been known in India since prehistoric times.
• Narandra Bael 5– Size – 1.25 kg/fruit, very thin rind 2-5 mm, TSS-38%, Slight acidity-25 mg/100g, eight years old plant- 56 kg.
• Narendra Bael 9 - TSS-40%, Ascorbic acid – 28mg/100g.
• Pant Bael 12 – Varanasi, Gorakhpur, Mirzapur, Etawa, Kagzi Sewan Large, Deoria Large, NB1, NB5 and NB9.
• Bael is very hardy subtropical, deciduous tree and can thrive well even in swampy alkaline soils having pH range 5 to 10.
• Major bael growing states: U.P., Bihar, West Bengal and Orissa.
• Seeds, which are sown in June, usually propagate bael; Seedlings are transplanted a year later.
• Patch budding is the month of June or July gives the best results.
• Bael can be classified as a climacteric fruit.
• Bacterial shot-hale canker of Bael is caused by (Xanthomonas blue).

Bael [Aegle marmelos (L) Correa.], a native fruit of religious importance, is well known to the Indian people for its nutritional and medicinal values. Leaves are used as sacred offering to ‘Lord Shiva’. All parts of plant viz., leaves, roots, bark, fruits, seeds etc. are used in preparation of various Ayurvedic medicines. Bael fruit is rich in protein, fat, minerals (Ca and Fe) and vitamins (riboflavin, β carotene and Vitamin C). Fruits are used to improve the digestive system and to cure stomach diseases. Fruits can be processed into various beverages and preserves (Jauhari and Singh, 1971). In spite of its Indian origin and high medicinal and nutritional values, well organized orcharding of bael is not commonly seen in the country. It is mainly found in wild and semi-wild condition throughout the India. Besides, it is also grown in the homestead gardens, backyards, religious places and farmer’s fields (Misra and Uniyal, 2013).

The bael (Aegle marmelos (L.) Corr.) is indigenous to India and it is also called as Baelpatra, Bengal quince (John and Stevenson, 1979), Indian quince, golden apple, holy fruit, bel, bilwa, bela, maredoo and other dialectal names in India. Hindus used the leaves to worship Lord Shiva (Bilwa Patra) and keep them on Shivling and they consider it is very auspicious and to get the blessings of Shiva. The fruits form a vital ingredient of holy offerings to the God and Goddess through holy pyre (haven). In history, the mention of bael tree is traced to Vedic times (C 2000 BC. to C 800 B..) within the Yajur Veda.

1. Downy Mildew (Green Ear)
Sclerospora graminicola
Diagnostic Symptoms
• Characteristic symptoms of the disease are pale, chlorotic, broad streaks extending from base to tip of leaves.
• At the advancement of disease, the leaf streaks turn brown and the leaves become shredded longitudinally.
• In severe infection, the downy fungal growth can be seen on the upper as well as lower surface of the leaves.
• Infected plants fail to form ear but if formed, they are malformed to green leafy structures.
• Complete ear can be transformed into leafy structure.
• Pathogen transformed all floral parts such as glumes, palea, stamens and pistils into green linear leafy structures of variable lengths and no grains are produced.
• Partial infection of ear head is observed in which some grains are produced and rest of the ear head is converted in to a green ear(Fig-1).

1. Introduction

Dairy industry size and demand precede all other segments of food industry. Reporting milk production of 146.3 million tonnes in 2014-15, India is currently occupying first position in world scenario (NDDB, 2015). In spite of limited shelf-life, milk offers various opportunities of product diversification, processing optimisation and convenient packaging solutions. All categories of dairy products are unique and vary in market value. The operating margins in value-added dairy products are almost twice of liquid milk business due to reduced need of refrigeration and added shelf-life. The appeal and satiety it offers makes them ambrosia for consumers. Baked and fried dairy products (BFDP) are those class of dairy products in which main processing operation is either baking or frying. In addition to preservation of milk solids for longer time at room temperature, manufacture of baked and fried dairy products add value to milk and also provide considerable employment opportunity (Pal and Raju, 2007;Patil, 2011). The characteristic flavour and colour development of the product rely on this unit operation or cooking method and it cannot be replaced. The unique difference in baked and fried dairy products is that in baking dough is prepared and for frying batter is prepared which vary significantly in characteristics (Table 1). The quality of the finished product greatly depends on how the dough or batter is handled.

Various types of equipment are needed and used to facilitate the process of baking. Depending upon the use, equipment may be as light equipment and heavy equipment.

Baking is no different from any other area of cooking, and as in other sectors only the best and the freshest raw material can guarantee good results. So selection of right kind of ingredients is of utmost importance. Another basic need of a professional baker and confectioner is to purchase the equipment required. The design and size depends upon the volume of sale expected. So in this chapter we are going to learn about the ingredients required for running a bakery.

Formulation & method of bread making Baking is an exact science that requires precise measuring and accuracy. Accuracy is crucial in baking because most baked products are made from the same basic ingredients: flour, liquid, fat, sugar, salt etc. the difference between baked products are often the proportion of each ingredients in the formula. If the proportions are off, you will end up with a different product or an unacceptable product.

1.    Major difference between unleavened and leavened bakery products is

(a) Unleavened do not form cracks on surface

(b) Unleavened do not rise significantly during baking

(c) Leavened bakery products are not attractive

(d) Unleavened products are much more stable

2.    Unleavened bakery products contain small holes throughout the finished product because of

(a) Ingredients contain fats that may swell

(b) Air incorporation between cells

(c) Incomplete coalescence of mixed ingredients

(d) High temperature baking