eChapters

Several attempts have been made to detect the biopotentials of microorganisms with reference to their ability to produce various enzymes with a view to use them in industrial processes and bioremediation applications. Enzymes are increasingly used as a new route to synthesize food ingredients such as flavours, emulsifiers, sweeteners, food additives, nutrients. With the advent of genetic engineering, a wide range of enzymes has become available on a large scale and this has increased the scope of enzyme technology.

MICROBIAL ENZYMES: Enzymes are proteinaceous complex organic catalysts of biological origin and vital constituents of cells. Microbes produce a variety of enzymes, intracellular and extracellular enzymes. Most of the microbial enzymes produced for commercial usage are extra cellular enzymes. Extracellular enzymes are secreted outside the cell and hydrolyze polymeric substances. Amylases and proteases are enzymes of economic importance. At present over 60 enzymes are in commercial use, of which three-quarters are hydrolytic enzymes. Commercial production of microbial enzymes utilizes a variety of fungi, yeasts and bacteria. The composition of the medium, genetic stability of the microbial strain and physical and chemical factors of fermentation influence the production of a particular enzyme.

Enzymes catalyze a variety of reactions during processing and preservation of food products and form a large group of processing aids. Food industry utilizes enzymes in the production of bakery items, dairy products, fruits and vegetables juices and products, tenderization of meat, modification of oils and fats, flavour production and preservation of food products. Enzymes have also found use in the manufacture of ingredients like glucose, high fructose syrup, citric acid, colour and flavour constituents.

Microbial contamination sources: Foods may be contaminated by microorganisms at any time during harvest, storage, processing, distribution, handling, or preparation. The primary sources of microbial contamination are soil, air, animal feed, animal hides and intestines, plant surfaces, sewage, and food processing machinery or utensils. Since most foods either carry or eventually acquire bacteria, molds, or yeasts, microorganisms are the major causes of food spoilage. The increased load of microorganisms leads to rapid spoilage.

Organisms: Bacteria and fungi (yeasts and molds) are the principal types of microorganisms that cause food spoilage and food- borne illnesses. The typical microorganisms that cause food spoilage are bacteria (e.g., Pseudomonas), yeasts (e.g., Candida), and molds (e.g., Rhizopus). The growth of microorganisms in the food will increase the load of microorganisms, and the heavier load will add to the difficulty of preventing spoilage and may influence the kind of spoilage to be anticipated.

Food spoilage may be defined as any change that renders food unfit for human consumption. These changes may be caused by various factors, including contamination by microorganisms, infestation by insects, or degradation by endogenous enzymes (those present naturally in the food). In addition, physical and chemical changes, such as the tearing of plant or animal tissues or the oxidation of certain constituents of food, may promote food spoilage. Foods obtained from plant or animal sources begin to spoil soon after harvest or slaughter. The enzymes contained in the cells of plant and animal tissues may be released as a result of any mechanical damage inflicted during postharvest handling. These enzymes begin to break down the cellular material. The chemical reactions catalyzed by the enzymes result in the degradation of food quality, such as the development of off- flavours, the deterioration of texture, and the loss of nutrients. The enzymatic changes in foods are desirable to some extent as in the case of ripening of fruits, but continued enzymatic activity beyond an optimum point becomes food deterioration due to rotting. The native enzymes may be inactivated by heat, radiation or by the use of specific chemicals.

10.1 CEREALS AND CEREAL PRODUCTS

CONTAMINATION: The sources of contamination in cereals and cereal products are soil, insects, air, etc. Most of the microorganisms are removed with the outer portions of the grain during milling. The bleaching agents used during milling also reduce numbers of microorganisms. The bacteria in wheat flour include Bacillus, Micrococcus, Flavobacterium. Mold spores are chiefly of Aspergillus spp and Penicillium spp and in addition Alternaria, Cladosporium and other genera are found. The numbers of microbes in cereals and cereal products vary widely from few hundred per gram to million. Corn meal contains several hundred to several thousand bacteria and mold per gram.

11.1. FRUITS-Maturation, Ripening and Storage

Fruits are natural foods containing substantial quantities of essential nutrients and the best sources of minerals like sodium, potassium and magnesium, vitamins (antioxidants), such as vitamin A, C and enzymes. They are rich in fructose and water. Fruits are living biological entities that perform a number of metabolic functions. Two functions of particular importance in fruit processing are respiration (the breaking down of carbohydrates, giving off carbon dioxide and heat) and transpiration (the giving off of moisture). Senescence is the period when chemical synthesizing pathways give way to degradative processes, leading to aging and death of tissue. Fruit ripening is thus the result of many complex changes, some interactive but many independent of one another. Once the fruit is harvested, respiration and transpiration continue, but only for as long as the fruit can draw on its own food reserves and moisture. It is this limited ability to continue vital metabolic functions that defines fruit as perishable.

CONTAMINATION

The healthy inner flesh of meat contains few or no microorganisms, although they have been found in lymph nodes, bone marrow and intestine. Staphylococcus, Streptococcus, Clostridium and Salmonella have been isolated from lymph nodes of red meat animals. During slaughtering the lymph nodes are removed from edible parts. The important contamination, however comes from external sources during bleeding, handling and processing. During bleeding, skinning and cutting the main sources of microorganisms are from the exterior of the animal- hide, hoof and hair and the intestinal tract. The exterior of the animal harbours large number and many kinds of microorganisms from soil, water, feed and manure as well as its natural surface flora.

Knives, cloths, air and hands and clothing of workers can serve as intermediate sources of contamination. During handling and processing the contamination can come from containers, air, other contaminated meat, equipments and personnel.

Molds that grow on the surface of the meat include the species of Cladosporium, Sporotrichum, Geotrichum, Mucor, Monilia, Penicillium and Alternaria. The important bacteria found in meat are Staphylococcus, Sarcina, Streptococcus, Clostridium, Leuconostoc, Lactobacillus, Proteus, Flavobacterium, Bacillus, Streptomyces, Escherichia and Salmonella.

13.1. CONTAMINATION

MICROFLORA: The flora of living fish depends upon the microbial contents of the waters in which they live. The slime in the outer surface of the fish contain bacteria of the genera: Pseudomonas, Alcaligenes, Micrococcus, Flavobacterium, Corynebacetrium, Bacillus, Sarcina, Serratia and Vibrio. The same species of bacteria are found in shrimps, crabs, lobsters and similar sea foods. Oysters and Shell fish that pass large amount of water through their bodies, pick-up soil and water microorganisms in this way including human pathogens. Common spoilage microorganisms of fish include species of Pseudomonas, Moraxella, and Acinetobacter, found mainly in marine fish, and Bacillus and Micrococcus, found in freshwater fish. Fish may also contain pathogenic (disease-causing) microorganisms such as Salmonella and Escherichia coli. Pathogenic contamination is of special concern with mollusks because they are often eaten raw and as whole animals.

SOURCES: Boats, boxes, bins, fish houses and fishermen are the sources of contamination of these bacteria. The bacterial population ranges from few hundred to few million per square centimeter. Because of their soft tissues and aquatic environment, fish are extremely susceptible to microbial contamination. At the time of harvest, fish carry a high microbial load on the surface of their skin, in their intestinal tract, and in their gills. The type and number of microorganisms that live in fish vary according to the season, the species, and the natural habitat. Additional contamination may occur during the harvesting, handling, or processing of the fish. Harvested fish must be immediately stored in a low-temperature environment such as ice or refrigerated seawater. This chilling process slows the growth of microorganisms that live in fish and inhibits the activity of enzymes. Because fish have a lower body temperature, softer texture, and less connective tissue than land animals, they are much more susceptible to microbial contamination and structural degradation. If immediate chilling is not possible, then the fish must generally be sold and eaten on the day of the harvest.

14.1. EGGS

India is the third largest producer of eggs. Fresh eggs are gathered on automatic collection belts at the farm and stored in a cooler at about 7°C. The eggs are then delivered to a central processing plant, where they are washed, sanitized, and graded. Grading involves the sorting of eggs into size and quality categories using automated machines. Flash candling (passing the eggs over a strong light source) detects any abnormalities such as cracked eggs and eggs containing bloodspots or other defects. Higher-grade eggs have a thick, upstanding white, an oval yolk, and a clean, smooth, unbroken shell.

14.1.1. Eggs Contamination

More than 90 percent of all eggs are free of contamination at the time they are laid; contamination with Salmonella bacteria and with certain spoilage organisms occurs essentially afterward. They become contaminated by faecal matter from the hen, by the lining of nest, by handling, by wash water and packing materials. The total number of microorganisms per shell of a hen’s egg was found to range from 100 to 10,000,000 with a mean of about 100,000. Proper washing and sanitizing of eggs eliminates most Salmonella and spoilage organisms deposited on the shell. The organism Salmonella enteritidis, a common cause of gastroenteritis (a form of food poisoning), has been found to be transferred through the hen ovary in fewer than 1 percent of all eggs produced. Ovarian- transferred S. enteritidis can be controlled by thorough cooking of eggs (i.e., until there are no runny whites or yolk).

15.1 CONTAMINATION

Milk is sterile at secretion in the udder, but contaminated by bacteria when it leaves the udder of a healthy cow. Milk is subject to contamination from the animal and adjacent areas. Bacteria found in manure, soil and water may enter milk from these sources. Certain diseases may be transmitted through milk, from a cow. It is essential that cows producing milk should be free from any contagious or infectious disease. Bovine tuberculosis comes from consumption of milk of tuberculous cows. Tuberculosis, brucellosis, mastitis and actinomycosis are the human disease transmitted through milk. The disease producing bacteria may find their way into milk and cause infectious diseases in children and adults.

COMMON BACTERIA IN MILK: The common bacteria found in milk are: Rickettsia, Coxiella burnetii, lactic acid bacteria, Lactobacillus bulgaricus, Streptococcus thermophilus, aerobic rods, Bacillus subtilis, Bacillus cereus, anaerobic bacteria, Clostridium butyricum .

Sources of Microbial contamination: Milk contains relatively few bacteria when it leaves the udder of a healthy cow. Milk is subject to contamination from the animal udder, skin and adjacent areas barn yard, milk utensils, milkers. Bacteria found in manure, soil and water may enter milk from these sources.

16.1. CANNED FOODS-PRESERVATION

Canning is a method of preserving food from spoilage by storing it in containers that are hermetically sealed and then sterilized by heat. The process was invented after prolonged research by Nicolas Appert of France in 1809, in response to a call by his government for a means of preserving food for army and navy use. Appert’s method consisted of tightly sealing food inside a bottle or jar, heating it to a certain temperature, and maintaining the heat for a certain period, after which the container was kept sealed until use. It was 50 years before Louis Pasteur was able to explain why the food so treated did not spoil: the heat killed the microorganisms in the food, and the sealing kept other microorganisms from entering the jar. Samuel C. Prescott and William Underwood of the United States set canning on a scientific basis by describing specific time-temperature heating requirements for sterilizing canned foods.

Most vegetables, fruits, meat and dairy products, and processed foods are stored in tin cans, but soft drinks and many other beverages are now commonly stored in aluminum cans, which are lighter and do not rust. Aluminum cans are made by impact extrusion; the body of the can is punched out in one piece from a single aluminum sheet by a stamping die. This seamless piece, which has a rounded bottom, is then capped with a second piece as its lid. The tabs used in pop- top cans are also made of aluminum. Bimetal cans are made of aluminum bodies and steel lids.

Canneries are usually located close to the growing areas of the product to be packed, since it is desirable to can foods as quickly as possible after harvesting. The canning process itself consists of several stages: cleaning and further preparing the raw food material; blanching it; filling the containers, usually under a vacuum; closing and sealing the containers; sterilizing the canned products; and labeling and warehousing the finished goods. Cleaning usually involves passing the raw food through tanks of water or under high- pressure water sprays, after which vegetable or other products are cut, peeled, cored, sliced, graded, soaked, pureed, and so on. Almost all vegetables and some fruits require blanching by immersion in hot water or steam; this process softens the vegetable tissues and makes them pliable enough to be packed tightly, while also serving to inactivate enzymes that can cause undesirable changes in the food before canning. Blanching also serves as an additional or final cleansing operation.

Food poisoning is an acute illness resulting from the ingestion of injurious agents such as chemical, heavy metals, parasites, microbial toxin, wild species of mushroom in food. Food poisoning is commonly caused by certain bacteria or their toxins, which are the poisonous proteins that these bacteria produce. Food borne pathogens are potential global problem, more than 90 per cent of the cases of food poisoning are caused by bacteria, Staphylococcus aureus, Salmonella spp, Clostridium perfringens, Campylobacter jejuni, Listeria monocytogenes, Vibrio parahaemolyticus, Shigella dysenteriae, Bacillus cereus and enteropathogenic E. coli. These bacteria are commonly found in many raw foods. Generally a large number of food poisoning bacteria must be present to cause illness. In most of these food poisonings, the food serves as a vehicle of transmission. The role of food here is significant, since the product not only may permit the survival of the pathogens, but also may provide a suitable medium for the rapid multiplication of microbes and production of toxin. A more serious aspect of microbial contamination is the possibility of serious health hazards due to potential pathogenic organisms gaining entry into foods due to low standards of hygiene and sanitation.

Food poisoning may be due to food intoxication or food infection. Food intoxication and food infection differ in many characteristics (Table 17.1). Food intoxication refers to food borne illness caused by the ingestion of a microbial toxin preformed in the ingested food (Botulism). The most serious food poisoning caused by toxins is botulism, an often fatal disease that results from eating improperly canned foods containing the bacteria Clostridium botulinum. Classically, food intoxication is caused by the growth of Clostridium botulinum, Staphylococcus aureus, Bacillus cereus in food. Clostridium botulinum produces a neurotoxin, while Staphylococcus aureus and Bacillus cereus cultures produce enterotoxins in food.

Fungi cause diseases in plants and animals and in such diseases, the fungi actively grow and invade the body of their hosts. There is another means by which fungi can cause harm is growing on stored food material that we consume and produce toxic metabolites referred to as mycotoxins (means fungus poisons). Food is particularly susceptible to fungal infections at almost every stage of storage or production. Mycotoxin is toxic substance produced by a fungus. The term is restricted to toxins produced by fungi growing in human foods or animal feeds. The growth of the fungi in food grains leading to physical spoilage of grains and toxin production is a serious problem in developing countries. Their occurrence in food and feed has both economic and health implications, as the toxic effects in human beings, animals and other living systems were well established. The effects of poisoning by mycotoxin are referred to as mycotoxicoses.

GENETIC ENGINEERING

Genetic engineering is a means of improving the food supply even before harvest or slaughter by improving yields, increasing disease resistance, and enhancing the nutritional qualities of various foods. Broadly speaking, genetic engineering refers to any deliberate alteration of an organism’s DNA. Genetic engineering has been practiced for thousands of years, ever since humans began selectively breeding plants and animals to create more nutritious, better tasting foods. In the past two decades, genetic engineering has become increasingly powerful as scientific advances have enabled the direct alteration of genetic material. Genetic engineering is alternately called as ‘ recombinant DNA technology’ or ‘gene cloning’. Genes have been cut and pasted from one species to another, yielding, for example, disease- resistant squash and rice, frost-resistant potatoes and strawberries, and tomatoes that ripen-and therefore spoil-more slowly.

Genetically modified organism (GMO) is a microbe or more complex plant or animal in which DNA has been altered for a particular purpose such as manufacturing proteins, making improvement to the organism, research into the nature of genes and biological process, correcting genetic defects.

Ex.No.1. MICROBIAL SPOILAGE OF FRUITS

Fruits are living structures that continue to perform metabolic reactions and sustain physiological processes for a considerable length of time after harvest. The post-harvest infection of fruits by microorganisms may cause serious losses of fruits both in terms of quantity and quality. Initial infection which may be the direct result of mechanical or insect damage is followed by invasion of fruit tissues by secondary infection by common saprophytic organisms. . Many bacteria and fungi, for instance, are involved in decay after harvest. Typical fungi include Alternaria, Botrytis, Colletotrichum, Fusarium, Monilia, Penicillium, and Rhizopus. These fungi are generally weak pathogens, in that they usually infest only weak or damaged fruit. Molds are the most predominant spoilage organisms in fruits. Fungal rots in fruits like apple show brown or cream coloured areas.

Bacterial soft rot caused by Erwinia carotovora is wide spread in fruits that are not very acid. Fruits like straw berries, melons are in direct contact with moist soil and become infected from that source.

To understand IoT “Internet of Things” it is important to understand internet first. Internet is connecting a device to other device anywhere in the world. When two devices are connected with the Internet, they can send and receive all kinds of information such as text, graphics, voice, and video. The high-speed, fiber-optic cables are used, through which the bulk of the Internet data travels. The Internet has revolutionized the communication world. In today’s scenario the internet is an important part for people around the world. The Internet is a global network of billions of electronic devices. The World Wide Web (www) is most widely used part of the Internet.

The “Internet of Things is a potentially integrated part of the ‘Future Internet’. IoT can be defined as a dynamic global network with self - configuring capabilities based on communication protocols where physical and virtual ‘Things’ interact with each other.

The Internet of Things (IoT) is inter-connecting the networks of smart devices. Smart devices may be embedded with vehicles, buildings, or other electronics object with software, sensors, actuators, and network connectivity. Network connectivity enables smart devices to collect and exchange data. The Internet of Things (IoT), also referred to as the Internet of Everything (IoE) which comprises of the web-enabled devices. IoT has its applications in smart city, smart surveillance, automated transportation, smarter energy management systems, water distribution, urban security and environmental monitoring. Agriculture is another new area of IoT application.

Due to advancement in technology the processing power and storage capacity has been increased, in the recent years. At the same time technology is making the devices pervasive, wearable and mobile. Smart devices are fitted with sensors, actuators which enable devices to sense, compute and act intelligently. They are able to exchange information with environment.

2.1 Introduction

Automation of agricultural field with IoT and microcontroller is new era of technology. Arduino based solution to agricultural field is open source platform. It can be used for developing data loggers and new sensors environment. The rate of solutions with Arduino and sensors is high as it is open source platform. The use of Arduino in agriculture can accelerate the growth of automation. Arduino can be interfaced with sensors like:

• Temperature
• Humidity
• Soil Moisture
• Water Pressure
• Water Flow and Water Metering
• Rain Gauge

3.1 Introduction

Internet of Things (IoT) allows computational devices and sensory support to connect with each other and access services on the Internet. The IoT idea was introduced to connect devices through the Internet and facilitate access to information for users. The potential application of IoT includes agriculture. The aim of this chapter is to explain the role of NodeMCU in the agriculture field. NodeMCU is IoT module which can be used to present the IoT concept as a basis for monitoring and control. The systems used in farm production processes. NodeMCU play a key role, with a focus on their realization by available microcontroller platforms and appropriate sensors. IoT based system provides opportunity to users to monitor and control the process remotely.

4.1 Introduction

The objective of this chapter is to design a mobile app for agricultural field automation with Blynk app. Mobile app is designed to control the water management in field as per requirement. The complete system comprises of two sections sensor node and mobile app. Sensor node comprises of Node MCU, Power supply, LCD, Relay board, soil sensor and Ultrasonic sensor. The system is designed to establish control and communication with specific agricultural field to take sensory data from soil and ultrasonic level sensor and control the PUMP IN motor and PUMP OUT motor with the help of mobile app.Fig.4.1 shows a block diagram for a system to control specific agricultural field with mobile app and IoT modem.

5.1 Introduction

The chapter describes the development of local web server on the laptop, to control the actuator from the server. Web server is used as remote control for the system. The system is designed to control water pump with respect to the soil sensor values. It comprises of NodeMCU, soil sensor, LCD, two relay boards for pump in and out the water from field.

6.1 Introduction

This chapter describes the development of data logger of agricultural field parameter monitoring system with LabVIEW. The system comprises of two sections- Fields device and data logger. Field device comprises of Arduino, smoke detector (for sensing hazardous gases), soil moisture sensor, temperature sensor, humidity sensor, water level sensor, battery, display unit, 2.4 GHz RF modem. Data logger comprises of Arduino, display unit, 2.4 GHz RF modem, power supply, PC with LabVIEW. The system is designed to monitor the environment and agriculture field parameters. The field device is designed to collect all physical parameters and communicate it to the receiver end with LabVIEW. For establishing communication between two sections 2.4GHz RF modem is used, which can be replaced with any other modem also.Fig.6.1 shows block diagram of field device and Fig.6.2shows block diagram of data logger.Table.6.1 & Table.6.2 shows the component list required to develop the field device and data logger.

7.1 Introduction

This chapter describes the development of data logger of agricultural field parameter monitoring system with MATLAB. The system comprises of Arduino, water level sensor, humidity sensor, light sensor, soil moisture sensor, temperature sensor, smoke detector, power supply, PC with MATLAB. The system is designed to monitor the environment and agriculture field parameters. The field device is designed to collect all physical parameters and same is communicated serially to MATLAB with I/O package. Fig.7.1 shows block diagram of the system. Table.7.1 shows the component list required to develop the system.

8.1 Introduction

The objective of this chapter is to develop a smart control for site-specific management of fixed irrigation system with Blynk app. Mobile app is designed to control the water management in field. The complete system comprises of two sections field device and mobile app. Field device comprises of Arduino, Node MCU, Power supply, LCD, Relay board, soil moisture sensor, temperature & humidity sensor, water level sensor, motor1, motor2. The system is designed to establish control and communication with specific agricultural field to take sensory data from the sensors and control the PUMP IN motor and PUMP OUT motor with the help of mobile app. Fig.8.1 shows a block diagram for a system.

9.1 Introduction

The objective of this chapter is to design an icloud and cayenne based perimeter monitoring system for agricultural field. Mobile app is designed to monitor the sensory data. The complete system comprises of two sections field device and mobile app. Field device comprises of NodeMCU, battery, motion sensors and laser sensors. The system is designed to monitor the agriculture field perimeter with motion and laser sensors, which are deployed at different locations of field. The sensory data is transmitted through NodeMCU and received at mobile app which can be accessed by authorized person. The same data can be accessed on webserver from anywhere in the world. Fig.9.1 shows a block diagram for a system. Table.9.1 shows the component list.

10.1 Introduction

The objective of this chapter is to develop a data logger and indicator for fire detection in agriculture field. The data logger is designed with the help of Scilab. The system comprises of Ardiuini Nano, light sensor, MQ6, MQ135, IR sensor, temperature sensor, power supply. The system is designed to take sensory data on Scilab with serial communication. Fig.10.1 shows block diagram for a system. Table.10.1 shows the component list required to develop the system.

11.1 Introduction

The application of greenhouse in agriculture is vast. Greenhouses are used for growing flowers, vegetables, fruits, transplants and varieties of certain crops in a controlled environment which is appropriate for the plants. The edible flowers are served in some good restaurants to grow them precise calculation is required. Sensor network can help to monitor and automate the activities in greenhouse. The objective of this chapter is to discuss the development of sensor nodes to monitor the growth of flower plants through XBee and internet of things. The system comprises of three sections- sensor nodes, local server and main server. Sensor nodes communicate to local server with XBee and then local server communicates the same data to main server through IoT.Fig.11.1 shows the generalized block diagram, sensor node is to be implemented on each flower pot and communicating to local and main server.

Presently the farming situation urges need to develop farming techniques, which are sustainable from environmental, production, and socio-economic points of view. Modern agricultural production throughout the world does not appear to be sustainable in the long run. Sustainable agricultural development “is the management and conservation of the natural resource base and the orientation of technological and institutional change in such a manner to assure the attainment and continued satisfaction of human needs for the present and future generations”. Such sustainable development in the agriculture, forestry and fishery sectors, conserves land, water, plant and animal genetic resources, is environmentally non-degrading, technically appropriate, economically viable and socially acceptable. Such concerns imparted a way to organic farming. It is the need of the day to understand the prospects and problems of organic farming to launch a successful and flawless organic production programme in the farm of environment.

Organic farming

It is the most widely recognized alternative farming system to chemical agriculture evolved during 1940’s largely in response to the publication of J.I Rodale in the U.S., Lady Eve Balfour in England and Sir Albert Howard in India. In 1980, USDA released a landmark report on organic farming. The report defined organic farming as follows;

“Organic farming is a production system, which avoids or largely excludes the use of synthetically compounded fertilizers, pesticides, growth regulators, and livestock feed additives. To the maximum extent feasible, organic farming systems rely upon crop rotations, crop residues, animal manures, legumes, green manures, off-farm organic wastes, mechanical cultivation, mineral bearing rocks, and aspects of biological pest control to maintain soil productivity and tilth to supply plant nutrients, and to control insects, weeds, and other pests”.

Manure is the organic material derived from animal, human and plant residues which contain plant nutrients in complex forms. Manures are used as source of plant nutrients. They release nutrient after their decomposition. They are applied in large quantities

Advantages of Organic Manures

• Organic manure provides all the nutrients that are required by plants but in limited quantities.
• It helps in maintaining C:N ratio in the soil and also increases the fertility and productivity of the soil.
• It improves the physical, chemical and biological properties of the soil.
• It improves both the structure and texture of the soils.
• It increases the water holding capacity of the soil.
• Due to increase in the biological activity, the nutrients that are in the lower depths are also made available to the plants through manures.
• It acts as mulch, thereby minimizing the evaporation losses of moisture from the soil.

Technical Knowledge (ITK) based special inputs / Techniques for plant growth

Growth of the plant has for long been believed to be due to the minerals absorbed from the soil and the food materials synthesized by the palnt. It is recognized that growth of the plant is very much regulated by certain chemical substances known as growth regulators. These substances are formed in one tissue or organ of the plant and are then transported to other sites where they produce specific effects on growth and development. These materials can be produced organically for improving plant growth.

3.1. Growth Promoting Substances

Are those organic solutions (like Panchagavya, Buttermilk & Coconut milk slurry, Amudhakaraisal) prepared using the plant and animal products, decayed fruit and other agricultural wastes. For example

Weeds can be considered a significant problem because they tend to decrease crop yields by increasing competition for water, sunlight and nutrients while serving as host plants for pests and diseases. Since the invention of herbicides, farmers have used these chemicals to eradicate weeds from their fields. Using herbicides not only increased crop yields but also reduced the labor required to remove weeds. Today, some farmers have a renewed interest in organic methods of managing weeds since the widespread use of agro-chemicals has resulted in purported environment and health problems. It has also been found that in some cases herbicides use can cause some weed species to dominate fields because the weeds develop resistance to herbicides. In addition, some herbicides are capable of destroying weeds that are harmless to crops, resulting in a potential decrease in biodiversity on farmers. It is important to understand that under an organic system of weed control, weeds could never be eliminated but only managed. In organic agriculture weeds are the resources as they are mostly native to the field/areas of existence (Children’s of the soil)

Insect pests cause huge losses ranging from 5 to 80% of even up to 100%. Acute food shortage following world war –II and Bengal famine (1943) due to failure of rice crop due to a paddy disease indicate the severity of the loss, caused by the pests and diseases. The insects in storage on an average consume and spoil an additional 4 million tones of grains every year. All this indicates the importance of plant protection by which we can save millions of tones of food grains which are otherwise eaten away by different pests. Losses due to insect pests in Indian agriculture are 23.3 per cent. One of the practical means of increasing crop production is to minimize the pest associated losses.

A pest is any organism which occurs in large numbers and conflict with man’s welfare, convenience and profit.

Pests are often controlled with man made chemicals which have many harmful effects, for example:

Indigenous Technical Knowledge (ITK) based inputs (Most of the ITK’s needs scientific validation before large scale adoption)

Is the systematic body of knowledge acquired by local people through the accumulation of experiences, informal experiments, and intimate understanding of the environment in a given culture (Rajasekaran, 1993). Learning from ITK can improve understanding of local condition and provide a productive context for activities designed to help the communities. In addition, the use of ITK’s assures that the end user of specific agricultural development projects are involved in developing technologies appropriate to their needs’. Yet, ITK is still an underutilized resource in the development activities. It needs to be intensively and extensively studied, and incorporated into formal research and extension practices to make agriculture and rural development strategies more sustainable.

Many of our indigenous practices in agriculture and allied fields have been replaced by the so-called modern technologies and they have become obsolete, especially among the younger generations. Now these indigenous practices are endangered ones and these are a possibility for them to become extinct particularly during this era of globalization, liberalization and commercialization. Now the need has come to re-examine and then gradually re-introduce the effective traditional technologies of crop production.

Therefore, there is a need to systematically document the ITK as they are the unwritten body of knowledge. There is no systematic record to describe what is, what it does, how it does, means of changing it, its operations, it boundaries and its applications. It is held in different brains, languages and skills in as many groups, cultures and environments. Tamil Nadu is also a treasure land of indigenous practices in agriculture. However, only a few attempts have been undertaken by social scientists to document the available indigenous agricultural practices but none has evaluated scientifically. The documented ITK practices are given below.

7.1. Rice

• Treatment of paddy seeds in diluted bio gas slurry for 12 hours increases resistance of seedlings to pests and diseases.
• During panicle formation in paddy, the flowers of Cycas circinalis are placed on sticks in paddy fields @ 4/ac. Its unpleasant odor repels ear head bugs.
• About 30 kg of tamarind seeds are applied for an acre of paddy field 1 day after transplanting to boost up the crop growth and yield.
• Soaking the paddy seeds in diluted cow’s urine before sowing, considerably reduces the incidence of leaf spot and rice blast
• Presoaking of paddy seeds in milk increases its resistance against ‘tungro’ virus and ‘stunt’ virus
• For control of red leaf spot disease in paddy, the seeds are soaked in ‘Pudina’ leaf extract (Mentha sativa) for 24 hours
• ‘T’ shaped bamboo stands are placed in many places in the paddy fields so that birds can sit on them and feed on the larvae and adults of rice pests.
• Sowing on eighteenth day (Aadipperukku) of Tamil month Aadi (Jul-Aug.) ensures good harvest.
• Daincha (Sesbania spp.) seeds are sown on paddy main fields when paddy nursery is raised and the grown up daincha is ploughed in-situ during field preparation.
• Plough the main field for four to six times for better yield.

8.1. Documented ITKs on Moisture Conservation

The ITKs were documented under following specific categories

• Agronomic Measures
• Tillage
• Bunding & Terracing (Mech. & Vegetative barrier)
• Land Configuration
• Soil Amendment / Mulching
• Erosion Control & Runoff Diversion Structures
• Water Harvesting, Seepage Control & Ground Water Recharge

9.1. ITKS for Seed Treatment and Sowing

• Yield of almost all the crops depends on seed quality.
• Even if it is a quality seed, it should be a dried one because well dried seeds will have higher longevity and keeping quality.
• It better to change the seeds at least once in two years.
• To maintain the seed viability and prevent it from outside damage, the outer shell is not separated.
• The seeds are generally stored along with the leaves of neem (Azadirachta indica), pungam (Pongamia pinnata), notchi (Vitex negundo) and thumbai (Leucas aspera).
• Six weeks nursery period is enough for six months duration of the crop.
• Shallow sowing is followed in dry lands.
• Severing the broadcasted seeds in dry lands with soil.
• Dragging, over the fields, the thorny branches with weight over them for covering seeds the sown on dry lands.
• It is better to start planting from ‘Sani moolai’ (north east) of the field to get higher yields.
• It is better to perform sowing and planting operations during evening hours.

Soil regeneration is about bulding or making topsoil. It is a particular form of ecological regeneration within the field of restoration ecology is the act/idea of replenishingthe top soil with beneficial nutrients casing natural methods. Time required from inorganic to organic would be about 60 to 200 days depending upon the situation. Once the inorganic fertilization is stopped, following actions facilitate quick conversion:

• Apply Farm yard/Poultry manure available in the farm as a basal dressing.
• Sow 25 kg of multi varietal seeds (MVS) in one acre.
• Allow it to grow up to 60 days.
• Inoculate the soil with the bio-agents like Azospirillum, Phosphobacterium, Pseudomonas flouresens, Trichoderma viride on 30 days after sowing (DAS).
• Incorporate in situ 60 DAS (30 t ha^-1 biomass is generated on 60 DAS) green manures.

Change in weather affects growth and development of plants. The extent of these effects depends upon the range or severity of change of weather. It is very much possible to change the weather in relatively small area to reap better harvest. Micro weather modification is defined as, “The manipulation of weather elements at increasing or decreasing the duration, intensity, quality etc., for desired effects at microlevel of crop under investigation”.

The climate near the ground can be managed partly by influencing the moisture status and temperature of the upper soil layers, e.g. by mulching and partly by action above the ground e.g. by shading or use of wind breaks.

12.1. General Practices for Storage

• Lime juice is mixed with grains and then sun dried before storage to prevent insect pests (Farmers in Nigeria).
• Seeds are safely stored in earthern pots after mixing with the leaves of neem and Vitex negundo (Farmers in Gujarat, India).
• Drying seeds and grains of all the crops on the new moon day before storing to avoid pest attack.
• Store seed materials in earthen pots which are kept on the ‘Paran’ directly above the furnace in kitchen. The smoke acts as a repellent.
• Grains, trees etc. harvested on full moon day are raqre prone to storage pest attack.
• Mixing the dried leaves of neem with seeds while storing them.

13 1. Rishi Krishi

Drawn from Vedas, the Rishi Krishi method of natural farming has been mastered by farmers of Maharashtra and Madhya Pradesh. In this method, all on-farm sources of nutrients including composts, cattle dung manure, green leaf manure and crop biomass for mulching are exploited to their best potential with continuous soil enrichment through the use of Rishi Krishi formulation known as “Amritpani” and virgin soil. 15 kg of virgin rhizosperic soil collected from beneath of Banyan tree (Ficus bengalensis) is spread over one acre and the soil is enriched with 200 lit Amritpani.

It is prepared by mixing 250 g ghee into 10 kg of cow dung followed by 500 g honey and diluted with 200 lit of water. This formulation is utilized for seed treatment (beej sanskar), enrichment of soil (bhumi sanskar) and foliar spray on plants (padap sanskar). For soil treatment it needs to be applied through irrigation water as fertigation. The system has been demonstrated on a wide range of crops i.e. fruits, vegetables, cereals, pulses, oilseeds, sugarcane and cotton.

14.1. Water Source Identification

• In places where Ficus religiosa tree is found, underground water will be more.
• Underground water will be more in places where the neem tree with more knots is found.
• In places where termite hills are found, underground water will be more.
• Farmers are practicing traditional method of using neem stick, metals etc. for finding the water resource in underground for digging wells. While they are walking with this stick on hand it will be rottate automatically at the place where underground water is surplus.
• Some farmers are using the magnetic stones for identifying water sources. Magnet is tied with a thread and held like a pendulum while walking over the fields. The magnet will rotate automatically at the place where underground water is more and this is taken as the indication for digging wells.

15.1. IFS Components Tested for Wetlands

Area Allocation;

In 1 ha of land

75 % - Cropping

10 % - Fodder cultivation

3 % - Goat shed

12 % - Fish pond

Possible Integrations:

Paddy is cultivated about 45 m ha in India, less than 1% is under organic cultivation.

16.1. Varieties

Good quality fine varieties like Ponni, Super ponni, insect and disease resistant varities must be selected for getting higher yield and returns.

16.2. Seeds

Organically produced seeds only must be used.

16.3. Seed Treatment

For 1 kg of seeds 20 g Azospirillum, Pseudomonas and 10 g Phospobacteria must be used with rice gruel.

Panchagavya spray @ 1% for treating the seeds.

Realizing that nematodes cannot be eliminated, and that we must live with them, the overall goal is to keep the population density as low as possible. The economic threshold is the density of nematodes where the losses incurred exceed the cost of nematode management. It is not advisable to depend on a single method to control nematodes. Efficient management requires judicious and careful integration of several which will result in reduction of nematode populations. Important components of non chemical methods of nematode management program are considered in this chapter.

17.1. Organic Amendments

Manure and fertilizer applications are essential part of growing any crop. Besides supplying required essential nutrients theyhelp in many ways directly or indirectly by suppressing harmful organisms including phytonematodes. The use of organic amendments against nematodes was demonstrated by Linford et al., in as early as 1938. FYM and cattle urine have found to reduce nematode population. Biogas plant waste slurry was found to be effective for nematode management. Compost of water hyacinth, oilcakes, cellulose amendments like sawdust, wheat straw, rice husk, rice hull ash, green manuring with legumes, green leaf incorporation are useful to keep nematode populations low. Non - edible oilcakes like those of neem, karanj, mahua, caster, cotton as well as edible oilcakes groundnut, mustard sesame, coconut have been recommended at 2 - 2.5 t/ha for reduction of root knot and other nematode population in soil. Miscellaneous substances like decaffeinated tea waste, rice hull ash, chicken manure, tannery and food and fruit processing waste, market and domestic organic refuse, dead vegetation and crop stalks, wood shaving, etc. have been recommended for root knot nematode management. Castor leaves (40g/kg) along with NPK at 75:25:37.5 kg/ha achieved reduction of M. javanica population on tomato.

In India, there are two accreditation systems for authorizing Certification and Inspection agencies for organic certification. National Programme on organic Production (NPOP) promoted by Ministry of Commerce is the core programme which governs and defines the standards and implementing procedures. National Accreditation Body (NAB) is the apex decision making body. Certification and Inspection agencies accredited by NAB are authorized to undertake certification process. The NPOP notified under FTDR act and controlled by Agricultural Processed Foods Export Development Authority (APEDA) looks after the requirement of export while NPOP notified under APGMC act and controlled by Agriculture Marketing Advisor, Directorate of Marketing and Inspection looks after domestic certification. Currently 20 certification agencies have been authorized to undertake certification process.

National Programme on Organic Production

National Program on Organic Production (NPOP) was launched during 2001 under the Foreign Trade & Development Act (FTDR Act). The document provides information on standards for organic production, systems criteria, and procedures for accreditation of Inspection and Certification bodies, the national organic logo and the regulations governing its use.

In food production remarkable progress has been made in the last fifty years in agricultural production and self – sufficiency (Food security) has been reached. Yet nutritional security seems to be a distant goal. With depleting natural resources and increasing threat to the environment, sustainable crop production is attracting the attention of the scientists, farmers and administrators. Due to over exploitation of natural resources and indiscriminate use of agro chemicals, green revolution also brought in quite a lot of miseries which focus the urgent need to have EVERGREEN REVOLUTION that is sustainable, leading to food and nutritional security to the rural poor. At this juncture it will be more appropriate to practicing organic agriculrure through the integration of traditional wisdom and modern scientific knowledge in synergy with nature.

19.1. Eco-friendly Technologies for Rice Cultivation

A sound package of eco-friendly technologies to grow rice is being successfully adopted by a few progressive farmers in Puliangudi village in Tirunelveli district of Tamil Nadu. “The technologies work well with indigenous rice varieties such as Kitchili Samba. The cost of cultivation is substantially reduced and the organic rice fetches a premium price in the market,” says Mr. P. Gomathinayagam, a pioneer in organic farming in Puliangudi.

The transition from conventional to organic farming requires numerous changes. One of the biggest changes is in the mindset of the farmer. Conventional approaches often involve the use of quick-fix remedies that, unfortunately, rarely address the cause of the problem. Transitioning farmers generally spend too much time worrying about replacing synthetic input with allowable organic product instead of considering management practices based on preventative strategies. Here are a few steps new entrants should follow when making the transition to organic farming:

20.1. Understand the Basics of Organic Agriculture and the Organic Farming Standards

Since organic production systems are knowledge based, new entrants and transitional producers must become familiar with sound and sustainable agricultural practices. Transitional producers should be prepared to read appropriate information, conduct their own trials and participate in formal and informal training events. As mentioned, switching from conventional to organic farming is more than substituting synthetic materials to organic allowed materials. Organic farming is a holistic system that relies on sound practices focused on preventative strategies. Since there are often few organic remedies available to organic producers for certain problems, prevention is the key element in organic production.

1.1. WEATHER AND TILLAGE

Weather : A state or condition of the atmosphere at a given place and at a given instant of time'

Climate : The generalized weather or summation of weather conditions over a given region during comparatively longer period

Meteorology : Greek word “Meteoro” means 'above the earth's surface' (atmosphere) and “logy” means “indicating science”. Branch of science dealing with that of atmosphere is known as meteorology

Agricultural meterology : Branch of applied meteorology or a branch of agriculture as it deals with agriculture

Coordinates of India : 8^o N and 37°N latitude; 68°E and 98°E longitude

Coordinates of Tamilnadu: 08°05' N and 13°35' N latitude; 76°15' E and 80°20' E longitude

Hydrosphere - water portion of the earth

Lithosphere - solid portion of the earth

Atmosphere - gaseous portion of the earth

Atmosphere : It is the colourless, odourless and tasteless physical mixture of gasses, which surrounds earth on all

Soil

Soil is a natural body of mineral and organic constituents differentiated into horizons usually unconsolidated, of variable depth which differs among themselves as well as from the underlying parent material in morphology, physical makeup, chemical properties and composition and biological characteristics (Whitney, 1982; Hilgard, 1892; Dokuchaiev, 1900 and Joffe, 1936).

According to Soil Science Society of America (SSSA, 1970) soil is the unconsolidated mineral matter on the surface of the earth that has been subjected to and influenced by genetic and environmental factors of parent material, climate (including moisture and temperature effects), macro and microorganisms and topography, all affecting over a period of time and producing a product, that is “SOIL” that differs from the material from which it is derived in physical, chemical, biological and morphological properties and characteristics. The unconsolidated mineral material on the immediate surface of the earth, that serves as a natural medium for the growth of plants.

Approaches of Soil Study

Pedological Approach : The origin of the soil, its classification and description are examined in Pedology. (From Greek word pedon means soil or earth). It is the study of soil as a natural body and does not focus on the soil’s immediate practical use.

Edaphological Approach: Edaphology (from Greek word edaphos means soil or ground) is the study of soil from the stand point of higher plants. Edaphologists consider the various properties of soil in relation to plant production.

Soil Science : The science dealing with soil as a natural resource on the surface of the earth, including pedology (soil genesis, classification and mapping) and the physical, chemical and biological and fertility properties of soil and these properties in relation to their management for crop production.

3.1. INTRODUCTION TO PLANT BREEDING

What is Plant Breeding?: Plant breeding is an art and science which deals with changing genotypes of the plants, to adapt them to the service of human being. (or) The application of genetic and cytogenetics principles in crop improvement are called plant breeding.

Specific objectives of plant breeding: The main objectives of the plant breeding is to improve the characteristics of crop plants which are agronomically and economically desirable

4.1. PESTS OF FIELD CROPS

Insect pests are the major constraints limiting agricultural productivity growth. It is estimated that herbivorous insects eat about 26 percent of the potential food production. Emerging problems of insecticide and disease resistance, secondary pest outbreak and resurgence further add to the cost of plant protection. Annual crop losses due to insect pests and diseases in India are estimated to be 18 percent of the agricultural output. Main causes of emerging pest and disease are favourable prevailing weather condition, absence of natural enemies, abundant food supply, use of pesticides, use of fertilizers and irrigation.

PESTS OF RICE

Borers and Defoliators

5.1. SEED AND SEED PRODUCTION

Origin of Plentiful Yield is the Seed - Parashara 400BC

Good Seed in Good Soil Yield Abundantly - Manu Smriti 200BC

Botanically seed is a matured ovule containing an embryo in arrested state of development with a food reserve (endosperm) and protective coat (seed coat). In albuminous seeds the endosperm is present eg. rice, wheat, castor (cereals). Whereas, exalbuminous seeds the endosperm is absent eg. onion and pea (pulses).

Types of fruit/ seed

6.1. PLANT WATER AND MINERAL NUTRITION

Plant water

Water form the major constituent of living (cells) things and the cells originated in a highly aqueous medium and all the vital processes of the life are carried out in it. Besides, water predominately acts as a source of hydrogen to plants and is released by the photolys is of water during photosynthesis.

In living tissue, water is the medium for many biochemical reactions and extraction process. Inorganic nutrients, photosynthesis, bases and hormones are all transported in aqueous solution. Soil nutrients are available to plant roots only when it get dissolved in water. It is essential for life and plays a unique role in virtually all biological process.

Water absorption

Plants absorbed water through root hairs which are in contact with soil water and form a root hair zone a little behind the root tips.Root hairs are tubular hair like prolongations of the cells of the epidermal layer of the roots. When roots elongate, the older root hairs die and new root hairs are developed so that they are in contact with fresh supplies of water in the soil.

Mechanism of water absorption

1. Active absorption of water : In this process the root cells play active role in the absorption of water and metabolic energy released through respiration is consumed active absorption may be of two kinds.