eChapters

Abstract

A field experiment was carried out during rabi 2012-13 at Bihar Agricultural College farm, Sabour, Bhagalpur to assess the effect of different herbicides on microbial population dynamics of soil in winter maize (Zea mays L.). The experiment comprised of ten weed management treatments involving various herbicides (acetochlor @ 1.125, 2.25 and 4.5 kg a.i. ha^-1, atrazine @ 1.0 kg a.i. ha^-1, pendimethalin @ 0.5 kg a.i. ha^-1 + atrazine @ 0.5 kg a.i. ha^-1, alachlor @ 0.5 kg a.i. ha^-1 as pre-emergence and 2,4-Diethyl ester @ 0.6 kg a.i. ha^-1and topramezone @ 0.04 kg a.i. ha^-1+ atrazine @ 0.25 kg a.i. ha^-1 as post-emergence including weed free and weedy check laid in randomized block design with three replications. Results indicated that all the herbicides’ treatment showed significant adverse effect on microbial population in rhizospheric soil of winter maize up to 45 days after sowing. Further, there was reduction in soil microbial population occurred in all the herbicides treated plots up to said time period as compared with weed free and weedy check. Initially the soil microbial population in weed free plot was much higher than that of weedy check, however, it was found in reverse trends in rest of the time intervals as well as harvest. Thereafter, soil microbial population was gradually increasing at different time intervals and at harvest, their population increased considerably in herbicidal treated plots though the weed free and weedy check showed higher soil microbial population than other herbicide treated plots.

Introduction
The majority of the world’s population consumes plant-based meals, which frequently lack important micronutrients and fail to satisfy recommended daily allowances (RDAs). Micronutrient inadequacy, also known as ‘hidden hunger,’ affects one in every three individuals worldwide (FAO, 2013). The growing worldwide population, expected to reach 9.8 billion by 2050 (United Nations, 2017), has increased the demand for high-quality food. Unfortunately, the emphasis on food quantity has frequently outweighed issues of food quality. Undernourishment is a major issue, especially in emerging and undeveloped nations where diets are inadequate and largely reliant on staple commodities such as grains (Datta & Vitolins, 2016). Poor lifestyle and dietary choices contribute to food insecurity (Gakidou et al., 2017). To address endemic nutritional deficits, fortification or supplementing techniques have been used (Bouis et al., 2017). Biofortification, which involves breeding nutrient-rich staple crop types, is a low-cost and environmentally friendly way to give important micronutrients. While biofortified staple foods may not contain as many minerals and vitamins as supplements or industrially fortified items, they do help to improve daily micronutrient intake throughout the lifespan (Bouis et al., 2011). Success stories of biofortification include lysine and tryptophan rich quality protein maize won World food prize 2000, Vitamin A rich orange sweet potato won World food prize 2016. It is vital to highlight that biofortification alone cannot completely remove micronutrient deficiencies; however, it can supplement existing initiatives and help vulnerable groups.

Introduction
The ecosystem recycles nutrients. Approximately forty chemical elements are thought to be necessary for the existence of living things. The earth’s system contains finite materials, hence the only way to maintain it functioning continually is to regenerate the components.
Definition
The term “biogeochemical cycle” refers to the periodic transfer of elements between living organisms (the biotic phase) and their non-living ,(abiotic) environments, such as rocks, water, and air. These cycles, which primarily involve water, oxygen, carbon, nitrogen, and phosphorus, are essential to life.
While micronutrients like Cu, Fe, and Co are derived from soil-based edaphic cycles, macronutrients like C, H, O, P, K, N, S, Mg, and Ca are cyclized with the atmosphere.There are two types of bio-geochemical cycles: gaseous and sedimentary cycles. The soil serves as the primary reservoir for nutrients in sedimentary cycles, such as the phosphorus and sulfur cycles, while the atmosphere and oceans serve as the primary reservoirs for nutrients in gaseous cycles, such as the nitrogen, carbon, and oxygen cycles. The biotic and abiotic systems are involved in both cycles. Both are fueled by energy flow and connected to either the hydrologic or water cycles.
• The nitrogen cycle, which involves gaseous components, is the naturalprocess by which nitrogen and nitrogen-containing molecules are transformed.

Abstract
Managing weeds in agricultural productivity poses a formidable challenge. An increasingly common weed control strategy in ecologically friendly farming systems is integrated weed management, which involves bioherbicides. Ensuring global food security and environmental safety heavily relies on the sustainability of agricultural production. Worldwide, sustainable agricultural approaches, including the maintenance of a permanent layer of soil cover, the minimization of soil disruption, the implementation of regular crop rotations, the practice of integrated weed control, and the adoption of conservation agriculture, are becoming increasingly popular. Bio-herbicides are commonly derived from plants containing phytotoxic allelochemicals or specialized bacteria that can induce diseases and reduce the population of weeds within a given area. However, bio-herbicides have demonstrated significant potential in inhibiting the germination and spread of weed seeds. Bio-herbicides offer a promising, environmentally friendly, promising solution for weed control, as they are derived from natural sources such as bacteria, plants, or biochemical compounds. This study investigates the effectiveness, environmental benefits, and challenges of using bio-herbicides to achieve sustainable weed control. Bio-herbicides possess the capacity to reduce dependency on artificial pesticides, eradicate unintended harm to non-target organisms, and foster a more harmonious ecosystem by employing biological agents to target particular weeds selectively.
Keywords: Bio-herbicide, allelochemicals, weeds, sustainable agriculture.

Introduction

In the middle of the 1900s, the world experienced a big increase in people, leading to a greater need for food. This demand for more food pushed for a farming revolution called the “green revolution.” This revolution used chemicals to help grow more crops and fight plant diseases. But using lots of these chemicals caused problems for the environment and people’s health. Even with all these chemicals, a large amount of food (up to 25% of what’s grown) is still lost to diseases each year. By 2050, we might need to double how much food we make. To do this without harming the environment or health, we’re looking for new ways. Some ideas include making plants that are genetically modified, creating plant varieties that resist pests naturally, and using helpful microorganisms like certain bacteria and fungi. These helpful organisms can be used in different ways, like making soil healthier or protecting plants.

The microorganisms colonizing plant roots generally include bacteria, algae, fungi, protozoa and actinomycetes. Enhancement of plant growth and development by application of these microbial populations is well evident. Among different microbial populations present in the rhizosphere, bacteria are the most abundant microorganisms. Various genera of bacteria, Pseudomonas, Enterobacter, Bacillus, Variovorax, Klebsiella, Burkholderia, Azospirillum, Serratia and Azotobacter, cause a pronounced effect on plant growth and are termed as plant growth promoting rhizobacteria (PGPR). PGPR play a significant role in enhancing plant growth and development both under non stress and stress conditions by a number of direct and indirect mechanisms. Microbes exert key functions in ecosystems being involved in nitrogen fixation, phosphorus solubilization, soil formation, and production of siderophores, plant growth regulators and organic acids as well as protection by enzymes like ACC-deaminase, chitinase and glucanase.

Bio-Inputs introduces the concept, scope, and functional diversity of biological inputs used in sustainable agriculture and natural farming. The chapter classifies bio-inputs by core components, including microorganisms, biological extracts, and biomolecules, and then by functions such as bio-fertilizers, bio-stimulants, biomejorators, and biological control agents. It also presents microbial and biological inputs within Integrated Pest Management, emphasizing biopesticides, biofungicides, and plant-defense elicitors. Examples such as Rhizobium, Trichoderma, Bacillus, Pseudomonas, seaweed extracts, vermiwash, neem extracts, and oligosaccharins illustrate how biological resources can improve nutrient availability, plant growth, crop protection, stress tolerance, and ecological sustainability. in contemporary agricultural practice. for sustainable crop production.

1. Microscope

The microscope is a precious instrument. There are many small objects or details of objects which cannot be seen by the unaided human eye.
 
A microscope (from Greek: mikrós, means “small” and skopeîn, means “to look”) is an instrument used to see objects that are too small for the naked eye.  In another word microscope is an optical instrument that uses a lens or a combination of lenses to produce magnified images of small objects, especially of objects too small to be seen by the unaided eye.

Introduction

Agriculture sector plays an important role in economy of India and contributes 18% to the GDP. It ensuring food security for more than1.26 billion Indian populations with diminishing cultivable land resource is a herculean task. Pesticides have been the most effective weapons and play vital role in crop protection against agricultural insect-pests. The green revolution technology has been characterized by use of hybrids/ improved varieties, chemical fertilizers, pesticides and irrigation water. After green revolution agricultural production has been very impressive but the excessive use of fertilizers and harmful pesticides has resulted in several undesirable effects on the environment and on the farming systems. However, indiscriminate use of synthetic chemical pesticides over the last four decades has adversely affected human health, non target organisms and environment and has also enhanced development of pesticide resistance among pest species While the former is exemplified by pesticide residues in soil, air, water, food etc., the latter includes phytotoxicity, physiological deform ities, diseases, mortality, population changes, genetic disorders, gene erosion, etc. in plant, mammal, avian, insect and other organisms. Entry of chemical pesticides into food chain and their bioaccumulation triggers several unforeseen consequences. However, in the conventional agriculture most of the weeds, pests, insects and diseases were controlled using natural and sustainable practices such as cultural, mechanical and physical control strategies. Environmental safety and agricultural sustainability are equally important for survival on the Earth. Therefore, it is the need of hour to identify the alternatives of suitable pesticides to control the insects-pests without sacrificing the productivity and profitability. Chemical pesticides has many side effects, eco-friendly management is essentially required for sustainable crop production in the present scenario.

The concept of insect pest management and the strategies employed have evolved over the time. With the increased emphasis on safe agricultural practices and elevated demand for safe and healthy food, the various solutions for pest management have shifted more towards use of biocontrol agents. Bio- rational pest management methods have now evolved as important pillar of the integrated pest management to replace the synthetic insecticides and also other associated advantages. They include the use of botanical pesticides, insect growth regulators, pheromones and allelochemicals. The major positive attributes associated with all of them are their low mammalian toxicity, safety to beneficial and non-target organisms, higher specificity, low risk of development of resistance and low environmental persistence. Though bio-rational insecticides are considered to be safe, in an organic ecosystem, it should be done with caution because over reliance on them for longer periods may facilitate the target insect pests to develop some kind of resistance against them. So, proper deliberations on the positive and negative attributes of bio rational pest management strategies should be done so as to facilitate their long term usage in organic farming.

The production of fruit and vegetables has recently increased significantly and even surpassed the total grain production in the country due to increased awareness of consumer and positive government initiatives (Schieber et al., 2001; Sagar et al., 2018; Malenica and Bhat, 2020). But due to limited production and area, the importance of excess production during a particular season and waste management arises at highest priority level. Moreover, fruits and vegetables waste are a serious problem for environment as most of themare being disposed of as landfills or directly disposed off to water bodies such as rivers (Wadhwa and Bakshi, 2013). Fruits and vegetables are perishable in nature and rich source of nutrients and vitamins, which results in microbiological contamination (Coman et al., 2019; Bas-Bellver et al., 2020). Foods wastes are usually organic residues generated by the processing of raw agricultural materials into food and are made up of liquid (wastewater) and solids (Bas-Bellver et al., 2020). The wastewater results from the cleaning processes or in the form of excessive or polluted process water. Its dry material content is typically less than 5% by mass. It possibly also contains organic or inorganic cleaning agents or disinfectants. Solid food wastes with an organic origin have remarkably high water content (mostly about 80% by mass). They are usually characterized by a constant quality and purity due to the forgone processes. The fact that these substances are removed from the production process as undesirable ingredients makes them, wastes.

1. Introduction

Malaria remains one of the most serious tropical diseases and kills an estimated one to two million people each year worldwide (Snow et al., 2005). Spread of drug resistance has played an important role in the occurrence and severity of epidemic diseases in the world (Majori, 2004). As a consequence of resistance to drugs such as quinine, chloroquine, primaquine and mefloquine, the vehemence of malaria has increased in many endemic regions of the world (Schlitzer, 2007; Basco et al., 1994). One of the biggest challenges to malaria control is the emergence and spread of drug resistant P. falciparum strains. Although human malaria transmitted by female Anopheles mosquitoes has four Plasmodium species as its aetiological agents P. falciparum, P. vivax, P. ovaleand P. malariae, the most widespread and severe disease is caused by P. falciparum, which transiently infects the liver before invading red blood cells of the mammalian host. Clinical manifestations occur at the erythrocytic stage and can include fever, chills, prostration and anaemia, as well as delirium, metabolic acidosis, cerebral malaria and multi-organ system failure, which may be followed by coma and death (Fidock et al., 2004; Deprez-Poulain et al., 2005, De Sa et al., 2009).The success of the natural product-based antimalarials such as quinine and artemisinin has showed the importance of plants as potential source of antimalarial agents. Consequently, numerous efforts to identify antiplasmodial activities from plants traditionally used as remedies for fever and malaria (Chen et al., 1997; Havsteen, 2002;

Abstract

Rice is the most produced and consumed source of stable food in J andK, and is excellent in genetic diversity. Rice production is the principal activity and a major source of income for the State. Traditional rice varieties have an enhancing potential in wide range of nutraceutical and functional foods. Besides possessing medicinal and nutritional properties, the by-products generated from these rice varieties in various post harvest operations are equally valuable. Traditional healers and local farmers have been using these traditional varieties in ayurveda and in curing of various kinds of ailments, such as cooling the body in ayurvedic treatments, improving vocal clarity, curing of boils, swellings and skin blemishes. Extracts of brown rice are also used as energy drink in individuals, patients and in treatment of chronic gastric problems, jaundice, dysenteric complaints and to increase lactation and nutrition to Childs. Sticky glutinous rice has been reported in treatment of stomach upsets, nausea, heart-burn and indigestion. Brown rice extracts have been reported in treatments of breast and stomach cancer and warts.

Introduction

Antioxidants are substances, which retard or prevent deterioration, damage or destruction caused by oxidation. Fortunately, the body has an army of antioxidants for damage limitation. Antioxidant form an integral part of the nutraceutical market. During the last few years’ research has confirmed that many have the common disease and ailments of 21^st century (Coronary Vascular Disease, diabetes, cataracts, high blood pressure, infertilities, respiratory infection, and rheumatoid arthritis) are associated with tissue deficiency and/or low dietary levels of compounds called antioxidants. The oxygen is consumed in the body during metabolism by process called oxidation. During oxidation free radicals are generated. These free radicals at a molecular level burn everything they touch. Antioxidants are power full electron donors and react with free radicals which damage the biomolecules. The formed antioxidant radical is stable and non-reactive. Antioxidants are quite large in number and diverse in nature which oppose the process of oxidation largely by neutralizing free radicals at relatively small concentrations and also have the potential to inhibit the oxidant chain reactions.

Abstract
Marine sponges are a rich source of bioactive compounds with diverse therapeutic properties, including antimicrobial, anticancer, anti-inflam-matory and antidiabetic effects. These organisms produce a wide range of secondary metabolites that help them survive in harsh marine environments and offer significant potential for drug development. The bioactive molecules derived from sponges, such as anticancer agents like Ara-C and novel antibiotics, are increasingly studied for their applications in pharmaceuticals and nutraceuticals. This review highlights the importance of marine sponges as valuable sources of functional ingredients with promising health benefits and therapeutic applications.
Keywords: Marine Sponges, Aquatic Environment, Drug Development, Oceanic Bioactive Compound

Introduction

Khejri (Prosopis cineraria) grows in dry and arid regions of Arabia and in regions of India mainly Rajasthan, Haryana, Punjab, Gujarat, Western Uttar Pradesh and drier parts of Deccan. The tree is locally known as Jandi or Khejri (India), Jand (Pakistan) and Ghaf (Arabic). It is the State tree of Rajasthan, India. Prosopis cineraria are small to medium size evergreen thorny tree. It is also known as “wonder tree” and “king of desert” as all the parts of tree are useful. The importance of the medicinal value of this tree has been highlighted in our ancient literature. The bark of the tree has abortifacient and laxative properties and is also used as a remedy for rheumatism in the central provinces. The leaves are of high nutritive value and locally called ‘’Loong’’. Leaf paste of P. cineraria is applied on boils and blisters, including mouth ulcers in livestock. The smoke of the leaves is considered good for eye troubles. Leaf extracts of P. cineraria have been reported to show antibacterial, antihyperglycemic, antihyperlipidemic and antioxidative activities . Pods are brown to chocolate in colour on ripening, each containing several seeds embedded in sweet dry yellow pulp. P. cineraria pods are locally called ‘’sangar’’ or ‘’sangri’’. The pod is considered astringent in Punjab. Sangri pods are known to prevent protein and mineral deficiency. Cooked pods of Khejri are used as a functional food in Rajasthan, for the amelioration of numerous illnesses. Pods of khejri have been studied for various phytochemicals like alkaloids, saponins, tannins, flavanoids, flavanols and total phenolics. As this plant is found in water stress (or deficient area) so antioxidant potential of pods has also been investigated. Acetone and methanol extracts of Sangri pods are the most potent bioactive extracts. Nutritional analysis shows it as a good source of proteins and minerals like calcium, sodium and potassium. This study demonstrates that P. cineraria pods may be employed as nutraceutical food with rich nutrition, disease prevention and health promoting effects.

1. Introduction 
Among the innumerable gifts of nature, plants, animals and minerals have been the basis of treatment of human diseases. Generally it is either of prebiotic origin or originating from microbes, plants or animal sources. More likely they are the natural expression of the increase in complexity of organisms. Beside animals, plants are one of the most important sources of natural products. From the very beginning of their existence, man has acquainted himself with plants and used them in a variety of ways throughout the ages. In search of food and to cope successfully with human sufferings, ancient man began to distinguish those plants suitable for nutritional purposes from others with definitive therapeutic action. Some plants are widely used as food while others showed beneficial effects against various human sufferings such as injuries and diseases. This relationship has grown between plants and man, and many plants have come to be used as drugs. The use of plant as a drug can be divided broadly into five periods. The early period covers the Indian, Chinese, Sumerian, Egyptian and Assyrian civilizations followed by the Greco-Roman, Arabian, Medieval and modern periods (Buchnman, 1980). Among ancient civilisations, India has been known to be rich repository of medicinal plants as the forest in India is the principal repository of large number of medicinal and aromatic plants, which are largely collected as raw materials for manufacture of drugs and perfumery products. About 8,000 herbal remedies have been codified in Ayurveda. The Rigveda (5000 BC) has recorded 67 medicinal plants (Chopra et al., 1956), Yajurveda 81 species and Atharvaveda (4500-2500 BC) 290 species. Charak Samhita (700 BC) and Sushrut Samhita (200 BC) had described properties and uses of 1100 and 1270 species, respectively (Kirtikar & Basu, 1918; Baquar, 1989) in compounding of drugs and these are still used in the classical formulations, in the Ayurvedic system of medicine. Before the availability of synthetic drugs, medicinal plants were used as the most primary source of medicine and continue to provide the mankind with new remedies for many diseases. These were used in crude forms like expressed juice, powder, decoction or infusion. As the plant have specialized biochemical capabilities they are able to synthesize and accumulate a vast array of primary and secondary chemical constituents or phytochemicals (Ghazanfar, 1994). Phytochemicals are responsible for metabolic activities and used for defense purpose. They are produced by specific biochemical pathways, which occur inside the plant cells. Due to their physical characteristic and chemical properties these chemical constituents are highly beneficial to the mankind and most importantly in primary health care. The first chemical substance isolated from plants was benzoic acid (Figure 1) discovered in 1560. The potent pain-killer, morphine (Figure 1) was separated from the dried latex of Papaver somniferum L. (opium) by F. W Serturner (1783-1814). The bioactive molecule, ephedrine (Figure 1) is one of the most potent plant based medicine and was isolated from Ephedra sinica (Ma huang). It was used in the form of various salts to combat bronchial asthma (Tang & Eisenbrand, 1992). Reserpine (Figure 1) an indole alkaloid, obtained from the roots of Indian plant, Rauwolfia serpentina, was used to treat hypertension. Taxol (Figure 1) is a diterpenoid first isolated from the stem bark of pacific Yew, Taxus brevifolia and used against ovarian for the treatment of ovarian cancer.

Summary

The present era is witness of understanding the science of human diseases at molecular level. The advances of science in terms of Genomics, Proteomics, Metaboliomics, Lipidomics and bioinformatics have drawn an excellent pathway to discover more promising drugs. It’s still a challenging task to reduce the time line to discover new drug, which is approximately 10-15 years. A drug discovery program requires billions of dollars but without the promise of success. After screening of thousands of compounds only one becomes successful in clinical trials and reaches to the market. Although there are lot many ways to approach drug discovery, nature has gifted many interesting compounds to fight the diseases. On the other hand isolation of pure compounds from natural products like bacteria found in soil or different parts of plants is itself a challenging area. The modern fashion of natural product isolation is guided by bioactivity. Hence a feasible assay to access the bioactivity has got a great importance.

Abstract

Milk is the almost complete and highly nutritious food for mammals, as it supplies all the energy and nutrients needed for the proper growth and development of the neonate. Milk proteins are considered the most key source of bioactive peptides and an increasing number of bioactive peptides have been identified in fermented dairy products and milk protein hydrolysates,. These bioactive peptides are sequences between 2 and 20 amino acids that improve the physiological functions and inhibit chronic diseases. The bioactive peptides are inactive within the sequence of the origin protein and can be released by addition of various types of proteolytic enzymes, coagulation enzymes and starter culture during milk processing. Once bioactive peptides are release in the body, these peptides may act as regulatory compounds with hormone-like activity. Furthermore, bioactive peptides from milk proteins have many biological activities such as antimicrobial, antihypertensive, antithrombotic, antioxidant, mineral binding, and anti-diabetic. Due to their physiological and physico-chemical variability, milk bioactive peptides are considered as greatly important components for health stimulating foods or pharmaceutical applications. Over the past few decades, major advances and developments have been achieved on the novel bioactive peptide and commercial applications of bioactive components which are present naturally in the milk. The aim of this chapter is to focus on the bioactive peptide present in milk, extraction of bioactive peptide, functional capability of bioactive peptides in milk and their importance in human health including bio active peptide based commercial dairy products.

1. Introduction
Aquaculture, the farming of aquatic organisms such as fish, crustaceans, mollusks, and aquatic plants, plays an essential role in global food security and economic development. As the demand for sustainable and efficient fish farming practices grows, the need to optimize fish health, growth, and feed efficiency becomes increasingly crucial. One emerging area of interest in aquaculture nutrition is the use of bioactive peptides, which offer a natural and effective alternative to synthetic feed additives.
Bioactive peptides are short chains of amino acids, typically ranging from 2 to 20 residues, which are derived from protein sources. These peptides are typically inactive within the parent protein but become bioactive once they are released through specific processes, including enzymatic hydrolysis, microbial fermentation, or gastrointestinal digestion. The peptides exhibit a wide range of biological activities that can directly benefit fish and shellfish health. Their functional properties include antimicrobial, antioxidant, immunomodulatory, antihypertensive, and growth-promoting activities, making them valuable components of aquafeeds.
The integration of bioactive peptides into aquaculture systems offers an innovative and environmentally sustainable approach to enhancing the health and performance of aquatic species. By replacing or complementing conventional feed additives, such as antibiotics and synthetic growth promoters, bioactive peptides can improve feed efficiency, disease resistance, and immune function, thereby promoting better overall productivity.

Introduction

For the past few decades, a number of chemical feed additives such as antibiotics, ionophores, methane inhibitors and defaunating agents have been introduced into ruminant nutrition in order to improve rumen fermentation, and increase growth and milk production by improving intake and feed conversion efficiency. However, most of these supplements are not used routinely because of toxicity problems to the host animals and microbial adaptation to these additives. Most importantly, growing awareness from public health aspects has centered on the safety of tissue residues of these chemicals and also bacterial resistance to antibiotics as a result of increased use in the food chain. These supplements have been criticized by the consumers’ organizations on the ground of product safety and quality. Antibiotic feed additives are banned in European Union. The consumers’ demands have drived the search for natural alternatives to chemical feed additives for eco-friendly animal production. Plants are part of herbivore diets, and these plants contain bioactive compounds with antimicrobial properties can be of interest in animal nutrition. Therefore, recent research has been greatly focused to exploit bioactive plant secondary compounds as natural feed additives to improve rumen fermentation such as enhancing protein metabolism, decreasing methane production, reducing nutritional stress like bloat, and improving animal health and productivity (Wallace et al., 2002; Patra and Saxena, 2009a, b; 2010; 2011), which may be useful in eco-friendly livestock farming (Patra, 2007). This review discusses the effects of different plant metabolites on rumen fermentation, rumen microbial ecosystems and ruminant performance.