eChapters

Toxic elements like Cadmium (Cd), Fluoride (F), heavy metals, radioactive metals are associated with mainly rock phosphates, other organic wastes like sewage sludge and pollutants. Their chemistry is similar to plant nutrients and replace them in their physiology leading to reduced uptake of nutrients, productivity and contaminated food chain.

In the realm of cell culture, the quality and composition of the growth media and reagents play a pivotal role in growth and sustaining cells in vitro. The preparation of these essential components requires precision, attention to detail, and adherence to the strict protocols to ensure optimal cellular growth, viability, and experimental reproducibility. This introduction serves as a foundational overview of the critical aspects involved in the preparation of media and reagents for cell culture experiments. Furthermore, the preparation of media and reagents for cell culture necessitates adherence to stringent quality control measures to ensure consistency and reproducibility across experiments. Validation of media batches, sterility testing, and periodic quality assessments are essential practic

 Objectives
• Understand the meaning of the communication process.
• Learn the various types of communication.
• Explore different models of communication.
• Analyze how communication flows in different contexts.
Meaning of Communication Process
The communication process refers to the systematic steps through which a message is conveyed from a sender to a receiver. It involves the transmission of information through a medium, with feedback confirming whether the message has been received and understood correctly.
Elements of the Communication Process
The communication process consists of the following elements:
• Sender: The person or entity initiating the message.
• Message: The content or information to be communicated.
• Encoding: The process of converting the message into symbols, language, or signals that can be understood by the receiver.
• Medium/Channel: The pathway through which the message is transmitted (e.g., face-to-face, email, phone).
• Receiver: The person or entity for whom the message is intended.
• Decoding: The process by which the receiver interprets and understands the message.
• Feedback: The response of the receiver that indicates whether the message was understood

In GADVASU, under the DBT sponsored project on “Development and evaluation of serodiagnostic assay for timely diagnosis and prognosis of mammary tumors”, we have generated and used hyper-immune sera raised against certain canine recombinant mammary tumor associated proteins for the standardization of sandwich and indirect ELISA for serodiagnosis of canine mammary tumor. The sensitivity and specificities of the assay’s ranges between 85-88% and 80-85%, respectively. However, an antibody against a single marker when used for different histotypes of CMT, its specificity reduced significantly. Multiplex assays are capable to detect many specific antigens in a single run whereas a traditional ELISA is used only to detect a single antigen. It has high-throughput potential, providing more results per sample, with lower sample volumes and with utmost sensitivity and specificity. Attempts are being made to increase the sensitivity and specificities of the assays by multiplexing various markers together (Fig.4).

Spirochetosis

Spirochetosis is caused by a spiral shaped organism Borrelia anserina and characterized by greenish diarrhoea, enlarged and mottled spleen, small necrotic patches on liver and linear haemorrhage in proventriculus. This disease is transmitted by a tick Argas persicus and thus also known as “tick fever” or “tick paralysis”.

Etiology

• Spirochete Borrelia anserina

• Spiral shape 8-24m long and 0.2 to 0.3 m wide microorganism having 8-11 spirals.

• Stained with aniline dyes

Principles
The quality of foods can be defined as the ‘composite’ of those ‘characteristics’ that differentiate individual unit of a product and have significance in determining the degree of acceptability of that unit by the consumer. The overall quality refers to technological, physiological, chemical, microbiological, nutritional; and sensory parameters to achieve the wholesome food. Sensory quality attributes such as taste, colour, texture, flavour and overall quality of any food products were evaluated by 9- point Hedonic rating test is used for the purpose of evaluation. This test measures the consumer’s acceptability.

Cell Lysis Procedure

Pellet the cells in a microfuge tube: add 1.5 ml culture, centrifuge for 15 seconds at maximum speed, discard the supernatant, and then repeat the procedure. This will result in the cells from 3 ml of culture in a single tube.

Resuspend the cells in 1 ml Lysis buffer.

Take 300 µl aliquot for Bradford protein assay.

Add 50 µl lysozyme, and mix gently.

After 10-20 minutes at room temperature, microfuge for 10 minutes to pellet cell debris.

Use supernatant for LDH assay.

Mammary tumors are the most common type of neoplasia found in female dogs with high percentage of malignancy and rate of mortality. The rate of incidence of these mammary neoplasia and dysplasia is continuously advancing nationally and internationally over the last 45 years. There are different risk factors such as age, diet, hormonal exposure and breeds, which are required to be monitored in order to control the incidence of CMTs. In addition to conventional therapies such as surgical excision, radiotherapy and chemotherapy, additional precision therapeutic agents targeting TKIs, p53 tumor suppressor gene, cyclooxygenases, epithelial-mesenchymal transitions and various hormones/ hormonal receptors could be beneficial for the treatment of CMTs. Scientific efforts are also being made to develop different biomarker panels and tests for prognosis/diagnosis of the diseases as well as to develop vaccines against CMTs.

1. The selection of more number of relevance variables could have added better understanding and comprehensive of the research.

2. Mathematical optimization and simulations could have been much effective in integrating as well as co-integrates the apparently different aspect of yield, income, livelihood and ecological resilience in the form of a common valley of management of a land.

3. Application of ARIMA (Autoregressive Integrated Moving average) and SEM (structural equation modeling) as instruments of interpretation and analysis can be more effective.

4. Ecological resilience metritis more active and dummy variable including outliers can be future research towards modelling with hard evidences

5. A pull of chronological data distribution over 30 years can undergo simulation and decision ecology for promulgating micro sociological policy and application.

6. Social resilience has been the predicament for perceiving ecology resilience and organic diode can help to make the entire thing strongly place on the foundation of ecological research at least.

Principle
Jam: Jam is a product made by boiling the fruit pulp with a sufficient quantity of sugar to reasonable consistency, firm enough to hold fruit tissues in position. Apple, pear, sapota, apricot, carrot etc. are used for preparation of fruit jam. Jam contains 0.5-0.6 % acid and invert sugar should not be more than 40 %.
Total soluble solids (TSS)
The TSS value is defined as the amount of sugar and soluble minerals present in fruits and vegetables. A refractometer is based on the principle of total refraction. This is done by placing a few drops of distilled water on the prism in the specimen chamber of the back the cover. The distilled water reading should be zero. If it is not so, it should be set to zero with the scale correction knob. The specimen chamber is now cleaned with muslin cloth or tissue paper. For determining the TSS, a drop of sample (jam, juice and syrup, etc.) is placed on the prism and the percentage of dry substance in it read directly on scale in refractometer.

In a large random-mating population both gene frequencies and genotype frequencies remain constant from generation to generation in absence of migration, mutation and selection and the genotype frequencies are determined by the gene frequencies. These properties of a random mating population were first explained by G. H. Hardy and W. Weinberg independently in the year 1908 and are generally known as Hardy-Weinberg law. The operational understanding of this law involves three steps as follows:

1. From parents to the gametes they produce.
2. From union of the gametes to the genotypes in zygotes produced.
3. From genotypes of the zygotes to the gene frequencies in the progeny generation.

The gene and genotype frequencies in the original parent generation are assumed as follows:

Plants have been used by man from prehistoric times to relieve from suffering and to cure various ailments. Primitive people, when injured in battle or when they had a fall or cut, they instinctively resorted to materials available at hand for staunching the flow of blood or for the relieving of pain and, by trial and error, they learnt that certain plants were more effective than others when used for these purposes. Man has also gained such knowledge from his observation of birds and animals that have been using plants for curing their ailments. Even today, we find that the domestic dog and cat, when they suffer from indigestion or other ailments, run to the field, chew some grasses or herbs and get cured. The folk medicines of almost all the countries of the world abound in medicinal plants and tribal people wherever they exist, rely chiefly on herbal medicine, even today.

Today, chemical and pharmaceutical investigations have added a great deal of status to the use of medicinal plants by revealing the presence of the active principles and their actions on human and animal systems. Investigations in the field of Pharmacognosy and Pharmacology have supplied valuable information on medicinal plants with regard to their availability, botanical properties, and method of cultivation, collection, storage, commerce and therapeutic uses. All these have contributed towards their acceptance in modern medicine and their inclusion in the pharmacopeias of civilized nations.

Principle
Jelly: Jelly is a semi-solid product prepared by boiling a clear, strained solution of pectin containing fruit extract, free from pulp, after the addition of sugar and acid. A perfect jelly should be transparent, well set, but not too stiff, and should have the original flavour of the fruit. It should be of attractive colour and keep its shape when removed from the mould. Pectin (0.5-1.0%), acid (0.5-0.75%), sugar (65%), and water are the four essential ingredients. Pectin test and determination of end-point of jelly formation are very important for the quality of the jelly. Some recipes for jelly making are given below:

Media Preparation

Before mixing medium, determine the quantity of each medium and the amount of plant growth regulator needed for each experiment. Make slightly more medium than is required. A media check off list is helpful in making medium to keep track of ingredients.

Remove media stock solutions and plant growth regulator stock solutions to be used from the refrigerator. Make up fresh stock solutions if adequate quantities are not available or if solutions have precipitated.

Plant growth regulators stock solutions are prepared biweekly and stored at 4º C in the dark. Cytokinins (thidiazuron and BA) are dissolved in approximately KOH prior the addition of deionized water. Gibberellins and auxins (2,4-D, IBA and NAA) are dissolved in 100% ethanol prior the addition of water.

Fill the volumetric flasks upto ½ to ¾ with deionized water. Add proper a of stock solution and plant growth regulators and mark each item on the check off list. Add 30 g/l sucrose, stir until dissolved. Make up the volume of the flask with deionized water and later por into a larger beaker.

3.1. Concept of farm mechanization

To apply the principles of engineering and technology to do the agricultural operations in a better way to increase crop yield.

Development, application and management of all mechanical aids for field operation, water control, material handling, storage and processing.

Mechanical aids include hand tools, animal drawn implements, power tillers, tractors, engines, electric motors, grain processing and hauling equipments.

A

Adenocarcinomas 3, 7

Adenomas 7

ALDH1 17, 18

Anaplastic carcinomas 7

B

Babesiosis 1

Bcl 17, 21, 23

Benign tumors 3, 5

Biomarkers 17, , 29, 30, 31

BRCA 31

C

Canine 1, 2, 5, 7, 8, 9, 10, 11, 12, 13, 17, 19,

19, 22, 23, 24, 27, 29, 30, 32, 33, 34

Canine distemper 1

Canine Mammary Tumors 1, , 5, 7, 8, 13, 17,

19, 29, 32, 33

Canine parvoviral diarrhoea 1

Carcinogenesis 10, 25

CHEK2 15

Classification and Grading 7

Companion animals 1, 9

COX inhibitors 21, 26

Cyclooxygenases 23, 26, 35

D

Deracoxib 21, 26

Diagnosis 19, 20

Doxorubicin 21

E

EMT 17, 25

Epidemiology 11

Estrogen receptors 20

FGFR2 15

Objective: Development of RTS beverage from seasonal fruits.
Material required
Fruit, juicer-mixer-grinder, preservatives, sugar, refractometer, pan and knife etc.
Principle
Ready To Serve(RTS): This a type of fruit beverage which contain at least 10% fruit juice and 10% total soluble solid besides about 0.3% acid. This is not diluted before serving.
Before undertaking the preparation of beverage, it is necessary to know techniques of extraction of pulp/juice from various fruits used for RTS.

Visit to a seed processing unit and attending the following questionnaire to know firsthand information on operations of shop floor