eChapters

Introduction

Biofortification is the development of nutrient dense staple food crops using the best conventional breeding practices and modern biotechnology, without sacrificing agronomic performance and important consumer preferred trait (Nestel et al., 2006). It provides a comparatively cost-effective, sustainable, and long- term means of delivering more micronutrients (Saltzman et al. 2014). This approach not only will lower the number of severely malnourished people who require treatment by complementary interventions but also will help them maintain improved nutritional status. Moreover, biofortification provides a feasible means of reaching malnourished rural populations who may have limited access to commercially marketed fortified foods and supplements. Micronutrient malnutrition (MNM) is the major constrain to provide nutritional security to world population of underdeveloped and developing countries. Among the 20 major risk factors of the global burden of disease estimates, Fe deficiency ranks 9th and Zn deficiency ranks 11th, while in the high mortality countries, including India, they rank 6th and 5th, respectively (WHO 2002).

Due to inadequate intakes of vitamins and minerals, half of the world's population suffers from micronutrient malnutrition. An adequate amount of wholesome as well as diverse foods are required for the proper growth and development. In addition to calories requirements, these foods should provide various nutrients. In India, ~70% of children do not get the required calories either due to lack of enough food or diverse food. This indicates that these children are also incapable of fulfilling their protein, minerals and vitamins requirements. Diets that lack diversity can be deficient in certain nutrients. Due to the micronutrient composition of the soil of a region, or because of the intrinsic insufficiency of the normal diet the staple foods of some region can lack particular nutrients. The individual plant foods are often deficient in certain nutrient components due to the varying level and composition of food nutrients in different food crops. Some crops are rich in one component but low in other. For example; legumes are usually high in protein, but deficient in essential amino acids like methionine whereas, wheat and rice are rich in carbohydrates but contain little essential amino acid such as lysine, iron, and lack provitamin A. Relying on a single food crop as major staple source of nutrients thus will not attain a nutritionally complete diet and result in malnutrition and deficiency diseases, which often occur in populations of developing countries, mainly due to poverty. Globally, over one billion people are undernourished and food security is a major issue with the world's population forecast reaching to nine billion by 2050.

1. Introduction According to the United Nations Food and Agriculture Organization, 780 million of the estimated 792.5 million malnourished people globally are in countries that are developing. Despite expanded agricultural production, “hidden hunger,” which is often caused by a shortage of vital micronutrients in daily meals, affects around 2 billion people worldwide. Majority of the population is relying on plant-based diets which are critical for sustaining nutritional security and are the primary source of nutrients required for optimal growth and development. Most of these diets are found deficit in major nutrients such as proteins, iron, calcium and Vitamin B12.However, a considerable number of Asian and African populations suffer from nutritional inadequacies as a result of consuming plant-based diets (Tripathi et al., 2019). Earlier, the main concern of farming systems was crop productivity and yield. However, there has been a shift in emphasis toward human health and well-being.

At this moment, the majority of energy is obtained from fossil fuels and requires switching over to alternative energy sources is the need of the hour. Fossil fuels are nonrenewable energy source and also have seriously negative impacts on the environment. The use of fossil fuels cause excessive global climate change because emissions of greenhouse pollutants and the formation of compounds CO_x, NO_x, SO_x, C_xH_y, ash and other organic compounds that are released into the atmosphere. The increase in greenhouse gas emission will result in global warming, climate change, environmental degradation and health problems.

Biofuel, any fuel that is derived from biomass - that is, plant or algae material or animal waste. Since such feedstock material can be replenished readily, biofuel is considered to be a source of renewable energy, unlike fossil fuels such as petroleum, coal, and natural gas.

Biogas typically comprises 60-65% methane (CH4), 35-40% carbon dioxide (CO2), and 0.5-1.0% hydrogen sulfide (H2S), along with traces of water vapor. It is approximately 20% lighter than air and has an ignition temperature of 650-750°C. The calorific value of raw biogas is around 20 MJ/m³ (4,713 kcal/m³).
Unlike Liquefied Petroleum Gas (LPG), biogas cannot be converted into a liquid state under normal temperature and pressure. The critical temperature for methane liquefaction is -82.1°C at a pressure of 4.71 MPa, making liquefaction complex and energy-intensive. However, biogas can be upgraded by removing impurities like CO2 and H2S and compressing it into cylinders for use in three-wheelers, cars, pickup vans, or stationary engines at remote locations.
Since Compressed Natural Gas (CNG) technology is already wellestablished, upgraded biogas (also called biomethane) which has similar composition and energy content can serve as a direct substitute for CNG in all its applications. The calorific value of upgraded biogas (with CO2 removed) increases to around 34 MJ/m³, compared to 20 MJ/m³ for raw biogas.
Techniques for Biogas Purification
To enrich biogas and increase its methane content, various purification methods are employed:
1. Water Scrubbing
This physical absorption process involves pressurizing biogas and feeding it into the bottom of a packed column, while water is introduced at the top. The CO2 and H2S dissolve into the water as it flows downward, while methane rises and is collected. The water used can

3.1 Introduction

Nature has a provision for destroying and disposing off wastes and dead plants and animals. This decay or decomposition is carried out by tiny micro-organisms called bacteria. Making of farm-yard manure (FYM) and compost is also through decomposition of organic matter (OM). When a heap of vegetable or animal waste and weeds etc., die or decompose at the bottom of backwater or shallow lagoons, bubbles can be noticed rising to the surface of water. Sometimes these bubbles burn with dancing flame at dusk. This phenomenon has puzzled man for a long time. It was only during the past hundred years that Scientists unlocked this secret as the decomposition process. The gas thus produced was and is still called “Marsh Gas.” The technology of harnessing this gas under artificially created conditions is known as Biogas Technology.

12.1 Introduction

Nature has a provision for destroying and disposing off wastes and dead plants and animals. This decay or decomposition is carried out by tiny micro-organisms called bacteria. Making of farm-yard manure (FYM) and compost is also through decomposition of organic matter (OM). When a heap of vegetable or animal waste and weeds etc., die or decompose at the bottom of backwater or shallow lagoons, bubbles can be noticed rising to the surface of water. Sometimes these bubbles burn with dancing flame at dusk. This phenomenon has puzzled man for a long time. It was only during the past hundred years that Scientists unlocked this secret as the decomposition process. The gas thus produced was and is still called “Marsh Gas.” The technology of harnessing this gas under artificially created conditions is known as Biogas Technology.

11.1 General

Biogas is generated through a process of anaerobic digestion of organic materials. The materials normally used are human and animal waste, crop residues, agro-industrial waste and other biomass in a combination with water. Biogas production technology contributes in following ways:

(a)It provides a better and cheaper fuel for cooking, lighting and for power generation.

(b)It produces good quality, enriched manure to improve soil fertility.

(c)It provides and effective and convenient ways for sanitary disposal of human excreta, improving the hygienic conditions.

(d)It generates social benefits such as reducing burden on forest for meeting cooking fuel by cutting of tree for fuelwood, reduction in the drudgery of women and children etc.

(e)As a smokeless domestic fuel, it reduces the incidence of eye and lung disease.

(f)It also helps in generation of productive employment.

The biogas technology is primarily based on fermentation of cellulosic rich organic matter under anaerobic conditions. The methane producing bacteria become more active after the creation of anaerobic conditions, thus, the gas produced become rich in methane & carbon dioxide, beside traces of hydrogen sulphide, ammonia, oxygen, water vapour etc. depending on the feed material and other conditions. For burning of gas, it is necessary that methane contents should be more than 50 per cent. The complete knowledge of process of methane production including its chemistry, physiology, bio-chemistry, micro-biology and bacteriology has been studied over years in many parts of the world. The culture and isolation of microbes under various environmental conditions have been successfully experimented to improve rate of reaction. The conditions, under which cattle dung, organic wastes, bushes, weeds etc. will produce methane, can be understood through chemistry of fermentation.

8.1 Introduction

Biogas is produced by the anaerobic digestion of biodegradable materials with the help of microorganisms. Biogas is also considered as renewable energy source because of is environment friendly nature. Conversion of cattle dung, kitchen wastes and agricultural wastes into biogas have the potential to minimize the burden on the fossil fuel and reduce the environmental pollution. Majority of developing countries specially their rural and suburban areas faces lack of access to the electricity and cooking gas. Biogas technology is emerging as a sustainable technology for waste treatment which could help to tackle the waste disposal issue in developing countries. 

According to Watson, bio-herbicides are living entities (natural enemies) used deliberately to suppress the growth or reduce the population of a weed species. This may include an insect; a microbe or a parasite nematode. These organisms operate directly or indirectly (by producing toxins) and their mode of action proves deleterious to the weed species. The latter toxins are termed bio rationals, phytotoxins or natural herbicides and are excluded in the definition.Bio-herbicides are formulations containing plant pathogens capable of mass production in vitro that are applied directly to target weeds uniformly to kill or suppress their growth. This method employs the annual release of a bio-agent into specific weed infested fields to infest and kill susceptible weeds. At present only the potential use of fungal pathogens as mycoherbicide has been studied in depth. Exploitation of bacteria seems promising where use of viruses as biocontrol agents are problematic since they are not host specific and require vector for their transmission.

Abstract

Hydrogen synthesis from bioprocesses a good approach for generation clean of fuel for world's development. Throughout the ancient and current periods, most of the developmental activities are completed by consumption of non-renewable fossil fuel energy that which has resulted in multiple environmental issues or challenges (Climate change and pollution by toxic gases or byproducts formation) at global level. Biohydrogen synthesis from anaerobic bacterial fermentation could be a good effort for sustainable energy production without any toxic byproduct formation. For production of hydrogen, lignocellulosic material can be utilized as cheap raw substrates. This would help the farmers in providing surplus benefits to farmer for their agriculture residues that normally burnt and cause the serious air pollution. Utilization of agro-waste residues can help in development of sustainable biofuels for world energy need. Burning of agrowaste residues by farmer's can be discouraged via utilization as raw carbon substrates thereby reducing air pollution.

Historical Aspect

The primitive man during his long history as a hunter and food gatherer, used ruminants as food till about 6000 B.C. Later, he developed the arts of aerable -agriculture and animal husbandry. The ruminants played a major role as sources ofbothfoodandmechanicalwork. In turn, the new techniquesof foodproduction greatlyincreased the amount of food available and enabledthe establishment of settled communities.Theradicalnatureof thesedevelopments was clearlyshown by the fact that within a couple of millennia, manhad evolved from primitive to urbanized, civilized communities. Throughout the historical epochs, animal food - meat, milk and milk products continued to form an important fraction of the diet in most of the civilized societies. The favoured nature of these foods was well shown in the observation that, with increasing affluence, human -beings tended to increase the fraction of the diet provided by animal foods, especiallyfromruminants.

Since middle of twentieth century, evidences had been accumulating to indicate that the high intake of saturated fats from meat and ‘dairy products might play a role in the etiology of atherosclerosis. This concern had provided a stimulus to produce husbandrytechniquesleadingto the modification of the composition of ruminant fats as well as possibility of protecting dietary poly - unsaturated fatty acids (PUFA) from ruminal biohydrogenation in 1951. Scientists all the world round, started studying ruiminant fats and ruminal lipid metabolism in this perspective. Different research groups developed various techniques of protectingdietary- PUFA, so as to make themavailable in the meat, milk and its products, but lacked the availability of sensitive techniques for confirmation. With the discovery of Gas Chromatoaraphy (GC) in 1954, a new beginning of resolution and detection of PUFA started with gradual precision. Later on, the GC was further equipped with mass - spectroscopy and subsequently with infra -red detector. The scientists were very much successful in protecting the dietary essential fatty acids (E.F.A.s) or even by - passing them from biohydrogenation in vitro.

The Distributed Information Sub Center at Kerala Agricultural University, Thrissur was set up in May 1995 as the 23^rd center of the BTISNet of DBT, Government of India. The area of specilisation of the Centre is Agricultural Biotech- nology. The Centre passed through several phases involving Internet connectivity through the ordinary C band connectivity, then the Ku band connectivity etc. Now the commu- nication facilities of the Centre have been strengthened by procuring an ISDN Internet connection and a connection through ASIANET. The Center caters to the needs of the students (UG, M Sc & Ph D) and faculty of all departments of the Colleges in the main campus as well as of the other nearby academic and research centers of the University. Over the years, DISC has come to occupy a position in the KAU in the area of Infor- mation Technology , information sources and services based on Internet, CD-ROMs etc. The Centre has procured databases in the field of Biotechnology and developed several databases in the field of Agriculture and related fields. The center regularly conducts training programmes in Bioinformatics. The research activities in the field of Bioinformatics have been enhanced. The Centre has now been upgraded to DIC to facilitate intensive research on Bioinformatics in an effective manner. During the year 2004, the Centre has also started offering a course on Bioinformatics for the M.Sc Biotechnology students of the College of Horticulture.

Biotechnology research in KAU spread over various research stations and cam- puses includes Plant Biotechnology, Animal Biotechnology and Fisheries Biotechnol- ogy. Thrust areas for Plant Biotechnology include developing in vitro protocols for mass multiplication of elite types of fruit plants, ornamentals, medicinal plants, spices, plantation crops and forest species. The other thrust areas include checking clonal stability and genetic fidelity of in vitro derived plants using molecular markers, DNA finger printing and characterisation of germplasm with molecular markers, imparting biotic and abiotic stress tolerance in otherwise accepted crop varieties, genetic trans- formation for imparting/ improving expression of desired traits, in vitro germplasm conservation, enhancement of secondary metabolites production etc.

The crops identified for biotechnology research at KAU include spice crops like black pepper, ginger, turmeric, vanilla, cardamom; plantation crops like cashew, coco- nut and medicinal & aromatic plants such as Kaempferia, Trichopus, Gymnema, Holostemma and Coscinium. For improvement of such crops through molecular breed- ing, it is highly essential to identify/design specific probes/primers, micro-satellites, etc. By combining the research results generated at the Biotechnology Centre and informa- tion retrieved from the Bioinformatics Centre, suitable probes, primers are being de- signed for characterization and improvement of these unique crops through molecular breeding.

Bioinformatics is a branch of biological science which deals with the study of methods for storing, retrieving and analyzing biological data, such as nucleic acid (DNA/RNA) and protein sequence, structure, function, pathways and genetic interactions. It generates new knowledge that is useful in such fields as drug design and development of new software tools to create that knowledge. Bioinformatics also deals with algorithms, databases and information systems, web technologies, artificial intelligence and soft computing, information and computation theory, structural biology, software engineering, data mining, image processing, modeling and simulation, discrete mathematics, control and system theory, circuit theory, and statistics.

Abstract

The completion of large number of genome sequencing projects has generated large volumes of raw sequence data. One of the fundamental tasks in bioinformatics is the creation of databases for data storage and development of tools for analysis. The first plant genome to be completed is that of Arabidopsis thaliana in 2000^[49]. This was followed by the rice genome and others. The availability of complete genomes has provided us with an opportunity to analyze them and understand plant biology in a holistic way. It has enhanced our understanding of factors affecting plant growth and development, abiotic and biotic stress, disease resistance, host pathogen interactions amongst others.

Abstract

Bioinformatics is a discipline combining biology, mathematics and the technology including computational tools and databases. It is one of the fast growing fields in the scientific community. This chapter is intended to provide an overview of the important databases of bioinformatics used for research in molecular biology, taxonomy, conservation biology, forensics and medicine and for the commercial applications in pharmaceutical industry and agricultural biotechnology. This chapter also describes various internet resources available in the field of Bioinformatics.

Introduction

Bioinformatics has emerged as one of the important scientific disciplines which revolutionized application of life sciences in our day to day life. A simple definition of bioinformatics is: “Bioinformatics is the use of computational techniques for the consolidation and analysis of large scale experimental biological data”. Hence, Bioinformatics has transformed the life science discipline from a purely wet lab-based science to a computationally assisted information science.

Application of bioinformatics in life science has multi facets right from gene discovery to drug design where the knowledge on sequence, structure and function of genes and proteins take the lead. The objectives of biological database generation are to: (i) organize the large scale experimental biological data for further investigation, (ii) make the biological data available globally and (iii) avoid duplication of experiments.

Bioinformatics is a new science conceptualizing biology in terms of molecules and then applying informatics techniques derived from disciplines such as computer science, maths and statistics to understand and organize the information associated with these molecules on a large scale (Gerstein and Jansen, 2000). It is often defined as the application of computational techniques to understand and organize the information associated with biological macromolecules (Luscombe et al., 2001). It represents a new field at the interface of the twentieth-century revolutions in molecular biology and computers (Pevsner, 2000)

Abstract

Indian agriculture made a rapid stride, converting the country from food scarcity to sufficiency. But challenges for the Indian agriculture in 21st century are much greater than before. The growing population has to be fed and surplus has to be produced with declining land, water and threat of climate change. Food and livelihood security of increasing population is a cause of concern and has received focused attention across the globe. Food security emphasizes access to sufficient, safe and nutritious food for an active and healthy life. Similarly, livelihood security has the emphasis on income and other resources to enable households to meet their fair needs.

The horticulture which includes fruits, vegetables, spices, flowers and medicinal and aromatic plants, has proved beyond doubt its potentiality for gainful diversification. Initiatives taken by Government and other stakeholders have impacted the development in terms of increased production, productivity and availability of horticultural crops. One of the significant developments is that horticulture has moved from rural confine to commercial production and this changing scenario has encouraged private sector investment in production system management. Now the horticulture sector contributes 29.65 per cent to agricultural GDP that has achieved a growth rate of 5-6 per cent during the decade. India has emerged as the second largest producer of fruits and vegetables and has first position in several horticultural crops. Production and export of flowers have increased manifold and the country has a major stake in global trade of spices and cashew nuts. Exports of medicinal plants, fruits and vegetables have also exhibited rising trend.

Bioinformatics is the study of analysis of biological data using computer programming, mathematics and statistics. Bioinformatics is the application of computer science and information technology to the field of biological sciences. Bioinformatics involves databases and information systems, web technologies, artificial intelligence and soft computing, information and computation theory, software engineering and statistics. The one of the most important achievement of bioinformatics is the human genome project which was mapped in 2001. Most of the work in this field is based on the research. Scientists make databases and develop different tools to analyze different gene expressions. Within last few decades, a number of research  in the field of molecular biology, along with advances in genomic technologies, have generated a huge number of biological information  Similar is the case in the field of animal production where many advances have occurred. The day to day advances in bioinformatics and computational science have led development in the field of animal as well as veterinary science. Bioinformatics generates new knowledge in the field of Animal Sciences, and improving & discovering new models of computation for the advanced livestock production.