eChapters

Pulses are widely acknowledged as superfoods, three times the protein in rice and double in wheat. Besides, they are also rich in complex carbohydrates, micronutrients, protein and B-vitamins and minerals like folate, iron, calcium, magnesium, zinc and potassium. High iron content in some pulses also minimizes the risk of anaemia. Long storability without loss of nutritional value, low prices, and broader availability make pulses an affordable source of minerals, and protein, thus contributing to food security at all levels of society in India. Besides human nutrition, pulse crops are also utilized as fodder and fuel for farm households.

Frankia is potent nitrogenous biofertilizer for forest trees since it establishes symbiotic association with non-leguminous plants. Nitrogen fixing non leguminous trees thrive in temperate condition. The species belong to Casuarinaceae are an exception and grow successfully in tropical areas. Alnus for temperate regions and the genus Casuarina for tropical and sub-tropical regions stand out examples of the benefits they provide to ecosystem by the way of nitrogen fixation. They have an excellent potential to adapt themselves to grow under diverse geographical and environmental conditions providing enough fuel, fodder, pulp and timber. The microsymbiont within these root nodules of non-leguminous plants has been identified and designated as Frankia (Becking, 1970).

Historical Developments

First of all Boussingault, a French farmer during 1838 drew attention that non-legumes were capable of fixing atmospheric nitrogen. The roots of Alnus were described as warty brownish biomass as large as man’s fist (Woronin, 1866). Frank and coworkers (1887a, b, 1891) studied the nodulated nitrogen f ixing trees regardless of whether they were leguminous or non-leguminous (actinorhizal). Frank believed that the bacteroids were protein deposits but subsequently he named the causative organism as Frankia subtilis. Moller (1885, 1890) reported the causative organism in the nodules as plasmodia and Frank also revised his opinion and pointed out that the causative organism was a fungus. Such conflicting results were due to imperfect techniques to differentiate between vesicles and sporangia. Later the organism was renamed as Frankia bruchorstii to honour Frank and his coworker, Brunchorst (Moller, 1890).

Fraudulent substitution of meat is the adulteration or mixing of the inferior or cheaper quality meat into superior quality meat. The first case of fraudulent substitution was recorded in thirteenth century A.D. at Florence in Italy. A common fraud in the meat industry in which uninspected meat is substituted for meat that has undergone inspection and been branded as satisfactory. The other frauds are the substitution of meat of another species, e.g. horse for beef especially in Britain and Ireland, beef in kangaroo meat in Australia, cat for chicken or rabbit, goat meat for mutton, mutton for venison, dog meat and cat meat for chevon in other countries including India. As per an estimate about 25-30% of meat sold in India is adulterated. These practices are more common in comminuted meat products. These illegal practices are punishable under PFA act 1955. The choice of method to be applied is depend on the need or condition of meat recovered.

Free carbon dioxide in the water accumulates due to microbial activity and respiration of organisms. Surface waters normally contain less than 10 mg free CO2 per litre while some groundwater may easily exceed that concentration up to 30 to 50 mg/L.
Principle
Free CO2 reacts with sodium carbonate to form sodium bicarbonate, or with sodium hydroxide to form sodium carbonate. Completion of reaction is indicated by the development of the pink colour characteristics of phenolphthalein indicator at the equivalence pH of 8.3.
Analysis of Water and Effluents
CO2 + N2CO3 + H2O = 2NaHCO3
CO2 + 2 NaOH = N2CO3 + H2O
Reagents
1. Standard sodium hydroxide titrant (0.05N): Dissolve 40g of NaOH in boiled CO2 free distilled water and make up the volume to 1 litre. Filter the solution through a sintered glass filter to remove any Na2CO3, the concentration of solution is 1N NaOH. Store it in in glass bottle.
2. Phenolphthalein indicator

Two procedure are described for the determination of free gosssypol . The first for normal meals and the second for meals which have been chemically treated and so contain dianilinogossypol.

Reagents
 
    1.    Aqueous acetone:  7 parts acetone, 3 parts distilled water (V/V).
    2.    Aqueous acetone-aniline solution: To 700 ml acetone and 300 ml distilled water, add 0.5 ml redistilled aniline. Prepare solution daily.
    3.    Aqueous isopropyl alcohol solution: 8 parts isopropyl alcohol. 2 parts distilled water (V/V).
    4.    Aniline Distill reagent grade aniline over a small quantity of zinc, dust discarding the first and last 10 percent of the distillate. Store refrigerated in a brown glass stoppered bottle. Solution is stable for several months.

Introduction

Diazotrophs are phylogenetically diverse microbes spanning over various classes varying vastly on basis of physiological properties (Young, 1992), include various phototrophic microorganisms aerobic phototrophic Cyanobacteria (Vaishampayan et al, 2001), anaerobic purple-sulfur phototrophs like Chromatium, and green-sulfur phototrophs, e.g. Chlorobium, Rhodopseudomonas; chemolithotrophs Alcaligenes, Thiobacillus, Methanosarcina, Methanobacterium, Desulfovibrio or Azospirillum lipoferum and heterotrophic bacterial anaerobes (Clostridium), microaerophiles (Herbaspirillum), and aerobes (Azotobacter). Free-living aerobic nitrogen fixing bacterial genera found in soil include obligate aerobes as Azotobacter, Beijerinckia, Derxia, Achromobacter, Mycobacterium, Arthrobacter and Bacillus but the majority are microaerophilic (e.g. Azospirillum, Herbaspirillum) or facultative and obligate anaerobes (e.g. Klebsiella, Aerobacter, Desulfovibrios, Rhodospirillum, Clostridium, Erwinia).

Freesia is ornamental flowering plant belongs to family Iridaceae subfamily Ixioideae. Freesia is named after a physician and botanist from Germany named “Friedrich H.T. Freese” (1795–1876). Dr. Christian P. Ecklon, a collector of plants from South Africa, named the flower after his friend and student. Modern freesias are known as Freesia × hybrida and cultivated for cut flowers. Freesia is grown for two purposes i.e. cut flower and pot plant.
 
It is excellent cut flowers because of its appealing shapes and found suitable for various types of arrangements and bouquets. Wide range of colour increases its beauty. Hence, its flower demand increased dramatically in recent year in global market. Forced freesias are grown as pot plant. Colours in freesias consist of pink, red, orange, yellow, blue, lavender, cream and white. Some flowers are multicoloured and possess several variations of colour within the same bud. Because of their delectable fragrance, Freesias have been drafted as a vital ingredient in many fragrance oils. Freesia fragrance oils are utilized for many oil burners and vaporizers. It is used as an essential fragrance in potpourris. Freesia essence oil is high grade essence oil. This is because it is undiluted, alcohol free, and long lasting. The oil is used for aromatherapy to scent candles, soaps, massage oils, bath oils and perfumes.

Freesia is ornamental flowering plant belongs to family Iridaceae subfamily Ixioideae. Freesia is named after a physician and botanist from Germany named “Friedrich H.T. Freese” (1795–1876). Dr. Christian P. Ecklon, a collector of plants from South Africa, named the flower after his friend and student. Modern freesias are known as Freesia x hybrida and cultivated for cut flowers. Freesia is grown for two purposes i.e. cut flower and pot plant.

It is excellent cut flowers because of its appealing shapes and found suitable for various types of arrangements and bouquets. Wide range of colour increases its beauty. Hence, its flower demand increased dramatically in recent year in global market. Forced freesias are grown as pot plant. Colours in freesias consist of pink, red, orange, yellow, blue, lavender, cream and white. Some flowers are multicoloured and possess several variations of colour within the same bud. Because of their delectable fragrance, Freesias have been drafted as a vital ingredient in many fragrance oils. Freesia fragrance oils are utilized for many oil burners and vaporizers. It is used as an essential fragrance in potpourris. Freesia essence oil is high grade essence oil. This is because it is undiluted, alcohol free, and long lasting. The oil is used for aromatherapy to scent candles, soaps, massage oils, bath oils and perfumes.

ORIGIN AND DISTRIBUTION

Freesias are South African in origin. Most species were found in the Cape Province area in southern Africa and two others were found just north of the equator in a tropical climate and even in Sudan. Freesia x hybrida is the name of modern Freesia.

In 1866 Freesia was described as a distinct genus. Freesia alba was first described in 1878 by G.L. Meyer, and became well known in Europe, as F. refracta var. alba. Gumbleton recognized Freesia alba as a distinct species in 1896, but Freesia alba first appeared in the English nursery trade in 1878 and quickly spread to Europe and North America. It appears to have caused quite a sensation, and appeared in almost every horticultural publication of note in both Europe and America in the years following its introduction. Breeding began immediately after F. alba appeared on the market and continues to this day.

Most of the modern hybrids were first made in the 19^th century from a few species. The genus contains some superbly ornamental and adaptable plants. These range from the lovely, scented Freesia alba and the brilliant red Freesia grandiflora and Freesia laxa, to the bizarre green-flowered Freesia viridis.

INTRODUCTION

Freesias are extremely popular bulbous plants that are best known as florist flowers. They bear bell-shaped tubular flower and are grown commercially on a vast scale. They are grown both for flower trade as well as in house gardens. Freesias are suitable for pots, shallow pans, beds, indoor decoration and window boxes. Freesia flowers are prized for their curious elegant form, peculiar arrangement of the blossoms on their stalks and a vast range of delicate colours ranging from creamy white to yellow, orange, red, purple, brown, lavender and vermillion. They are highly valued cut flowers.

Freesia flowers are so strongly scented that only a few potted or cut blossoms can bring fragrance to an entire room. Its unusual appealing shapes make them suitable line flowers for any arrangement. They also make fragrant additions to bouquets and their wide range of colours increases their versatility for design uses.

The process for freeze dried flowers began in 1989. Freeze drying was first used by the Peruvian Incas of the Andes, who stored their food on the heights of the Andes. The ancient Incas were the first to learn the benefits of sublimation. They discovered that when they dried food at high elevations, the results were better. Even though the food would freeze, it would dry well if left exposed to the sun. The reduced pressure at high elevation, combined with the dry air and energy from the sun, had caused sublimation to occur.

Food preservation methods are important in order to avoid glut or shortage of food in the market. In addition it helps to increase the shelf life, maintain the nutritional and sensorial quality, facilitate transportation and improve the overall value of the commodity. Even though various methods of food preservation are available now. Most of the conventional methods are thermal preservation methods which may result in the loss of volatile nutrients and flavour, different sensorial modifications, microbial spread with or without gas production, damage to the microstructure, rupture of coating or packaging surfaces and microbial recontamination caused by non-hermetic closure.

Abstract

Drying is a widely used method of food preservation. It leads to some structural changes in the dried material resulting in poor quality and rehydration. Freeze drying, though a costly process, reduces water activity without exposing the food material to high temperatures, eventually retains the original quality to a great deal. Moisture in the foods is frozen to ice which is sublimated under low pressure. Low pressure helps to vaporise the ice without transforming it to liquid, hence leaving the food structure porous in nature. The food is claimed to preserve the nutritional, sensory and re-constitutional qualities.

1.1 INTRODUCTION

Most food materials are composed of a very high percentage of water, which can be as high as 95%, and this makes them susceptible to microbial activity. Microbial activity spoils the products, leaving them useless for human consumption. It not only changes their taste and color but it may also develop toxins, thus making them unsafe. There are various methods for preserving food but in order to make them shelf stable, removing water from foods helps keep them from spoiling for a longer period of time.

15.1. Introduction

Freezing is one of the oldest and most widely used methods of food preservation, which allows preservation of taste, texture, and nutritional quality in foods better than any other method. It is a process of bringing down the temperature of food below its freezing point and the frozen storage generally refers at temperature below -18oC.

15.2. Theory of freezing

The freezing process is a combination of the beneficial effects of low temperatures at which micro-organisms cannot grow, chemical reactions are reduced, and cellular metabolic reactions are delayed. Freezing is generally referred to as a unit operation in which the temperature of food is reduced below freezing point and a proportion of water undergoes a change in state to form ice-crystals. Immobilisation of water to ice and the resulting concentration of dissolved solutes in unfrozen water cause lowering of water activity of the food. Thus reduction in water activity and use of low temperature coupled with some pre- treatments like blanching is the basis for food preservation by freezing. Fruits such as strawberries, oranges, raspberries, black currants and vegetables like green pea, green beans, sweet corn, spinach, sprouts and potatoes are examples of commercially frozen products.

Introduction
Freezing is one of the oldest and most widely used methods of food preservation, which allows the preservation of taste, texture, and nutritional quality in foods better than any other methods. The freezing process is a combination of the beneficial effects of low temperatures at which micro-organisms cannot grow, chemical reactions are reduced, and cellular metabolic reactions are delayed. Freezing is generally referred to as a unit operation in which the temperature of food is reduced below the freezing point and a proportion of water undergoes a change in state to form ice crystals. Immobilization of water to ice and the resulting concentration of dissolved solutes in unfrozen water cause a lowering of water activity of the food. Thus, reduction in water activity and use of low temperatures coupled with some pre-treatments like blanching is the basis for food preservation by freezing. Fruits such as strawberries, oranges, raspberries, black currants, and vegetables like green peas, green beans, sweet corn, spinach, carrots, sprouts, and potatoes are examples of commercially frozen fruits and vegetables.
The frozen food market is one of the largest and most dynamic sectors o the food industry. Despite considerable competition between the frozen food industry and other sectors, large quantities of frozen foods are being consumed all over the world. The frozen vegetable industry mostly grew after the development of scientific methods for blanching and processing in the 1940s. The commercialization history of frozen fruits is older than frozen vegetables. The commercial freezing of small fruits and berries began in the eastern part ofthe United States in about 1905.

52.1. Aim

To conduct practical on freezing of fruits.

52.2. Theory

Freezing is a method of preservation in which the food temperature is reduced below freezing point and a proportion of water changes in to ice-crystals. Immobilization of water to ice and the resulting concentration of dissolved solutes in unfrozen water cause lowering of water activity in the food. Thus, reduction in water activity and use of low temperature coupled with some pre-treatments is the basis for food preservation by freezing. The method for freezing of fruits depends upon the intended use. The process variables for freezing of different fruits and flow chart are shown in Table 52.1 and Fig 52.1. Generally fruits after preliminary treatments are packed in sugar syrup and frozen in freezer. The fruits are frozen to an internal temperature of -18oC or lower and kept at 18oC or lower throughout transport and storage.

Freezing is one of the most critical stages in ice cream manufacture because it creates the frozen structure while simultaneously incorporating air. This chapter explains the principles of dynamic freezing and the structural changes that occur as the mix is transformed into semi-frozen ice cream. It discusses ice-crystal formation, air incorporation, overrun, draw temperature, fat destabilization, and product condition at freezer draw. Different freezer systems and hardening requirements are examined, along with calculations related to output and overrun. The chapter also introduces ingredient feeders and ripple or ribbon systems. Understanding freezing technology is essential for controlling texture, body, meltdown, yield, and overall product quality.

53.1. Aim

To conduct practical on freezing of vegetables

53.2. Theory

Freezing is often considered the simplest and most natural way of preservation for vegetables. Frozen vegetables and potatoes form a significant proportion of the market in terms of frozen food consumption. The quality of frozen vegetables depends on the quality of fresh products, since freezing does not improve product quality. Pre-process handling, from the time vegetables are picked until ready to eat, is the important factor affecting quality of finished product. The process variables for freezing of different vegetables and flow chart are shown in Table 54.1 and Fig 54.1. Generally fruits after preliminary treatments are packed in sugar syrup and frozen in freezer.

INTRODUCTION

Preservation of food by drying or chemical means as by alcohol or acid fermentation and that by the use of ice is long recorded in history. With the advancement of science and the knowledge governing various unit operations in food processing, these food preservation techniques employing thermal, radiation, or low temperature or a combination of these have only got further refined as regards their viability and commercial applications. Process foods of different genre and cuisine, which include frozen foods too, are increasingly becoming popular the world over for reasons of quality, safety and year round supply of nutritious foods to cater to different niche segments of human society. Increasing health consciousness is buoying those processed foods which are preserved and stabilized using non-thermal technologies as these ensure retention of food nutrients to a better definition. Interest in frozen food also stems from need to deliver safe and nutritious foods with extended shelf-lives.

Freezing is one of the easiest and least time consuming methods of food preservation. The freezing process is a combination of the beneficial effects of low temperatures at which microorganisms cannot grow, chemical reactions are reduced, and cellular metabolic reactions are delayed.

Methods of Freezing

1. Sharp freezing (slew freezing)

In sharp freezing commodities are exposed at -15 to -29⁰C. It required 3-72 hrs. Here the thawed tissue cannot regain its original water content. The ice crystals formed are large and rupture the cells. The thawed tissue cannot regain its original H₂O content.

1. Introduction

Freezing is a method of food preservation that involves lowering the temperature below the freezing point of food generally -18 °C or below. During freezing of food the water present in them changes its state to form ice crystals. Human beings in the colder regions have used freezing as a means of preservation since they learnt hunting and gathering foods. The foundation of the modern frozen industry was laid in mid 1920 when Clarence Frank Birdseye, an American inventor used cold brine solution to chill packaged fish and extended it further for quick freezing of meat poultry fruit and vegetables. 

The cotton aphid :Aphis gossypii: Aphididae

Both nymphs and adults cause the damage by sucking the plant sap from shoot tips, flowers and leaves.  Severe infestation may result in complete drying of affected parts.  Attacked leaves curl up, wither and fall. These are yellowish green or brown coloured aphids.  The total life cycle from first instar nymph to adult occupies an average of 8-10days.  It reproduces parthenogenetically and viviparously.  The female may produce 10-30 young ones in a life span of 10-12 days.  The nymphs transform into adult in 5-8 days after passing through four moults.

Botany: French bean has tap root system with poor nodule formation. Leaves are trifoliate. Though a self-pollinated crop, French bean offers wide variability for plant growth (bushy / climbing), colour of pod (green / waxy coloured), cross section of pod (flat / oval / round), pliability (stringed / string less) etc.

Varieties

1. Arkakomal: Plant is bushy. 70 days for flowering.
2. Blue pod medium: It is a white seed variety. It has bluish black spots on pods.
3. Bountiful: Introduction from US comes up in September to February. Seeds are Brown sweetish. It is resistant to common disease.
4. Contender: Tolerant to powdery mildew and mosaic.
5. Pant anupama: Developed from pantnagar. Bushytype
6. Pusaparvathi: Developed by X ray radiation of American variety IARI

Among the pulses, rajmash is one of the high potential pulse crops with a yielding potential of 18 to 20 q per ha. Rajmash or French bean (Phaseolus vulgaris L.) is a short duration non–traditional grain legume and one of the precious and highly relished pulse crops of North India. Rajmash is known by various names viz., French bean, rajma, haricot bean, field bean, kidney bean, snap bean, pole bean etc. It is an important winter vegetable grown both for tender pods and dry seeds, which form a rich source of crude protein (21.25%), fat (1.7%) and carbohydrates (70%). Besides, it contains 0.16 mg iron, 1.76 mg calcium and 3.43 mg zinc per 100 g of edible part (Jasvinder Kaur and Mehta, 1994). The fresh pods and green leaves are used as vegetable. The antimetabolites of dry beans needs removal by cooking and soaking in water.

Among beans, the most popular bean is French bean and it has wider distribution and larger gene pool. Various names are associated with cultivars viz., Snap bean, salad bean and green bean and all these names associated are with beans of vegetable types. The names haricot bean, dry bean and navy bean are applicable to pulse types. String bean, dwarf bean and pole bean applicable to growth forms. It is known as Rajmah in Hindi, Tingalawari in Kannada and Fras bean in Punjabi. Cultivars preferred for Canning, frozen and freeze-dried are having round pods. Fresh market cultivars are flat or oval shaped. Yellow pod cultivars are also grown. Generally fresh market cultivar yields higher in temperate region than in tropical region. Beans are said to be antidiabetic and good for bladder burns, cardiac, carminative, diuretic.

Origin and Distribution: Phaseolus vulgaris was domesticated in Central America about 7000 years ago and it is also thought to be domesticated in Brazil and Northern Islands and was introduced to Europe in 16th century. Vavilov (1951) reported that Phaseolus vulgaris originated in South Mexico and Central American centre of origin. The first cultivar has been selected in Peru. From Peru it spread to Europe and to Asia. Wild species P. aboriginus in North West Argentina is considered as the wild progenitor of French bean. Based on growth habit, crop can be classified into pole type, semi pole type and bush type. Former varieties are more string types. American origin of P. vulgaris is questioned by De Candolle (1584). Southern part of Mexico and Central America are considered to be primary centre of origin. While secondary centre lies in Peru-Bolivian-Ecuador of South American continent. In Europe, French bean spread rapidly in 16th and 17th centuries and reached England by 1594. From Europe, it was introduced to India during 17th century (Simmonds, 1976).

French bean (Phaseolus vulgaris L.) is a nutritious protein-rich legume vegetable crop cultivated in all parts of the world except Antarctica. Immature pods are consumed as vegetables, and matured pods are used as grain legumes (Allem, 2013). French bean is cultivated in 1.65 million hectares of area with a production of 26.98 million tonnes and productivity of 16.36 tonnes per hectare for vegetable purposes. As a grain legume, French bean was cultivated in 33.02 million hectares with a production of 28.90 million tonnes and productivity of 874.1 kg/ha during 2019 globally. In India, French bean is grown in 2.56 lakh hectares for vegetable purposes and 126.91 lakh hectares for grain legume purposes with a production of 7.26 lakh tonnes as immature pods and 53.10 lakh tonnes of grain legumes with a productivity of 2.83 t/ha of green pods and 418.4 kg/ha as grain legumes (area, production and productivity of livestock, Food and Agriculture Organization of United Nations, 2020). However, the area, production and productivity statistics of beans in FAO statistics may also include other beans such as mung bean and others (Sharma et al., 2013). French bean is also known by other names such as common bean, kidney bean, dwarf bean, haricot bean, string bean and garden bean. In India, French bean is also known by different names as Frash bean and Rajmah in Hindi, Shravanghevada in Marathi, Thingalaware in Kannada and Fras bean in Punjab.

French bean, Phaseolus vulgaris under the family Fabaceae (Leguminosae) and having chromosome No.2n = 2x = 22 is an important leguminous vegetable crop grown worldwide for its tender fruit (pod) as vegetable and dry seeds as pulse. French beans are unique among plant foods due to their high protein content. It is a highly variable species that has a long history of cultivation. Most of the bean varieties for processing (canned, frozen, and freeze-dried) are round podded, while fresh market cultivars are often flat or oval shaped. French bean is a valuable source of the amino acids lysine and tryptophan; minerals likes, iron, copper and zinc; and beneficial phytochemicals, antioxidants and flavonoids.
Origin, domestication and adaptation in India
French bean is thought to have been originated in South and Central America. Southern Mexico and Central America are considered to be the primary centre of origin, while secondary centres lies in Peru-Bolivia-Ecuador region of American continent. It was actually domesticated in Mesoamerica first and travelled south, probably along with squash (Cucurbita species) and maize. Multi-locus sequence data have indicated that the domestication of common bean was initiated 8000 years ago. Cultivation of French beans started by the American Indian tribes settled in Tehuacan Valley of Mexico and in Callejon de Huaylas, Peru. When Christopher Columbus returned from his second voyage to the New World in the year 1493 he brought French beans with him in the Mediterranean region. French beans were considered to be rare to find and expensive but soon became one of the commonly used beans in the 19th century. In France, French beans were introduced in the year 1597 by the Conquistador (Spanish explorers). In Europe, the French bean spread rapidly in the 16th and 17th centuries and reached England by 1594.

The frequency distribution of the populations of M₂ and M₃ generation for each genotype was extracted according to Panse and Sukhatme (1961). Skewness and Kurtosis for all the characters were calculated as per the standard method. Skewness refers to the symmetry of the frequency distribution. A measure of skewness is obtained as making use of the second and their moments about the mean. It is defined as,

Frequency distribution table is a statistical table which shows the sets of all distinct values of the variables arranged in order of their magnitude either individually or in groups with their corresponding frequencies side by side.

Need for frequency distribution table

1. To condense the data
2. To provides quick reference for the entire data
3. To facilitate quick & easy statistical analysis of data
4. To reveal the pattern within the data

10.1 Introduction

There are very few frequency modulated fiber optic sensors. This is because frequency modulation of light occurs under a limited range of physical conditions. The principal one is based on Doppler effect. There are few other circumstances under which the frequency of light is modulated. These include luminescence and Raman scattering. Luminescence has been included in chapter 7under fluorescence based sensors. In this chapter we shall focus on the sensors based on Doppler effect and Raman scattering. In particular, we shall discuss the measurement of blood flow velocity, colloidal particle motion and Raman spectrum using optical fibers.

1. What are Primary produce?

Primary produce, also known as primary products, refers to raw materials or natural resources that are derived from agriculture, forestry, fishing, or mining activities. These products are typically unprocessed or minimally processed, and are often used as inputs in further manufacturing or production processes. Examples of primary produce include crops such as wheat, corn, and soybeans, as well as livestock such as cattle, sheep, and pigs. Forestry products such as timber and paper pulp, as well as fish and other seafood harvested from the ocean or inland waterways, are also considered primary produce.

1. Choose the most appropriate option with a tick mark

i) Floating plants are required for breeding

a) Guppies

b) Angel fish

c) Swordtail

d) Gourami

ii) Breeding traps are generally used for

a) Egg-scatters

b) Egg-depositors

c) Livebearers

d) Nest-builders

1. A production of 600 tones/ha/year of tilapia in cages has been recorded in Laguna Bay in Philippines.

2. Indian Major Carps (IMC) contributes 84% of India’s aquaculture production.

3. The seed production of the carps has been standardized, with commercial hatcheries producing over 19000 million fry per year.

4. A pit like structure located near the outlet of the pond is called as harvesting pit (measures about 50 - 100m2).

5. Size of the nursery pond = 12 x 6 x 1m.

6. Size of the Rearing pond = 25 x 12 x 1m.

7. Size of the production pond is 0.1 to 2 ha.

8. Size of the quarantine pond is less than 0.5 ha.

9. During construction to retard erosion and seepage, soil must contain a minimum of 25% of clay.

10. The depth of the pond must be 0.5 to 1m.

Introduction

The science of Biotechnology has been making every effort to create new opportunities for the welfare of humanity. Modern biotechnological tools are applied in India to boost fish production through aquaculture as well as conservation of the vast aquatic resources of the country for sustainable utilization. Transgenic technology is widely used in biotechnology, from the generation of genetically modified (GM) foods to the production of pharmaceutical proteins. This technology has been successfully used to develop fast growing super-fish stocks for aquaculture. The main reasons for genetic manipulation of species used in aquaculture are to enhance the growth and efficiency of food conservation, to increase tolerance to environmental variables like temperature and salinity, to produce new colour variants of ornamental fishes, to increase the resistance of species to pathogens, etc. Though the conventional selective breeding approach has proven to genetically upgrade rohu19, it is a rather time consuming process. Traditional selective breeding approaches in combination with transgenesis can bring about faster improvement of selected traits.

Introduction

Culture of aquatic organisms is a significant economic endeavor that benefits millions of people worldwide by generating employment opportunities and ensuring food security. One of the lucrative aquaculture ventures in many countries is the cultivation of freshwater pearls. Prior to the development of cultured pearl farming, pearls were harvested from the Gulf of Mannar, the region between India and Sri Lanka, the Bay of Bengal, the Egyptian coast (Red Sea), and the Persian Gulf (Saudi Arabian coast) (Matlins 2001; Strack 2008), which benefited the nation’s economy. However, the natural pearl fisheries have been depleted as a result of indiscriminate fishing, habitat destruction, and pollution. Realizing its economic significance, pearl farming has developed into a successful aquaculture activity to support the nation’s aqua-economy through innovation and standardization of farming techniques.

Pearls are cultured in both marine and freshwater environments, and they are particularly created in bivalves with an inner nacreous layer. Many countries like China, Japan, Australia, Indonesia, French Polynesia, Cook Islands, Philippines, India, Sri Lanka, Bangladesh, Myanmar, Thailand, Malaysia and Mexico are major pearl producing countries through farming (Gervis and Sims 1992; Southgate 2007; Southgate et al. 2008 & Saurabh et al. 2021). In addition to the economic benefit like other bivalves these pearl producing mussels has positive environmental impact and also has several nutritional benefits. Freshwater bivalves including these pearl mussels are widely used in aquaculture for aquaculture-effluent treatment of aquaculture farms. The shells of bivalves are the source of two highly versatile chemical compounds, calcium carbonate or calcium oxide which is having wide industrial applications. The shells are used in cosmetics and traditional medicine, handicraft and jewelry. These economic and environmental benefits have catalyzed expansion of the bivalve aquaculture industry in various regions of world. During 2009 to 2018 the average freshwater pear cultured production was 1928 tonne while China continued to dominate world freshwater pearl culture (FAO, 2020). However, the freshwater pearl culture production has declined from 3321 tonne in 2009 to 703 tonne in 2018. This drastic decline may be due to overexploitation, habitat degradation, pollution, lack of proper management and many more and it has threatened long term sustainability of fisheries and this farming sector. It gives an alarming call for all fisheries managers and policy makers to formulate management measures to implement for the betterment of the sector. Though this farming technology has a great potential to increase the aqua economy of the country, growth should be sustainable.

Introduction

Pearls have been favourite jewel of humanbeing since ancient times. Pearl is a natural gem and is produced by living organisms, the pearl oysters and mussels (Molluscs), unlike other precious stones. It is perhaps, the only gem that does not require the treatment by lapidary. According to our Indian system of gemmology, of the nine maharatnas (Heera, Mukta, Manikya, Pravala/Munga, Gomeda, Indra-neela, Vaidurya, Pushyaraga, and Panna) pearl (Mukta) is only next to diamond (Heera). The science of pearl culture has not been widely popular, however, the art and trade form has reached the zenith.

With the turn of the last century, the demand for pearls increased, but their supplies from the existing fishing grounds have diminished due to overexploitation and pollution. Such a situation necessitated tremendous research attention in the following decades with support from state and trade sectors, the world over. This led to the evolution of culture pearl technology as a booming aquaculture enterprise in Japan and China. These two countries have a monopolistic control over pearl trade.

Introduction
Freshwater pearl farming is an emerging and promising sector within India’s aquaculture industry, offering significant potential for economic growth and sustainability. As the country modernizes its agricultural and aquacultural practices, the cultivation of freshwater pearls presents a lucrative opportunity for farmers, particularly in regions where traditional agriculture faces challenges. Recognizing its importance, the Indian government has integrated pearl farming into its broader Blue Revolution initiative, which seeks to enhance the fishing sector and improve livelihoods through sustainable practices.
China and Japan are the main producers of freshwater and marine pearls, respectively, whereas China is the world’s biggest producer of pearls, including both marine and freshwater pearls, with 3540 tonnes produced, accounting for 98% of global pearl production. Bangladesh, Korea, Thailand, and Vietnam, have recently begun both research and industrial-scale programs in response to the worldwide commerce potential of farmed freshwater pearls (Fassler, 1994). The history of pearl farming in India dates back to 1969 with the stablishment of the Central Institute of Freshwater Aquaculture (CIFA), which initiated the cultivation of freshwater mussels for pearl production. This effort has gained momentum over the years, with states like Gujarat, Maharashtra, and Rajasthan becoming key players in the industry. Farmers in these regions are increasingly adopting pearl farming as a viable and profitable alternative to
conventional agriculture. India is home to over 50 species of mussels found in various freshwater ecosystems.When a foreign body becomes lodged within the mantle (the skin) of pearl mussels or oysters, the mantle secretes a nacreous substance in response to the irritation. This process results in the formation of layer-by-layer nacre coating over the intruding object. Nacre consists of
80-90% calcium carbonate (CaCO3) and a protein called Conchiolin. As this sector develops, freshwater pearl farming not only promises to contribute to the economic growth of local communities but also positions India as a competitive player in the global pearl market. By addressing existing challenges and continuing to support innovation and education, the future of freshwater pearl farming in India looks increasingly bright.

Introduction

Majority of the commercially important freshwater prawns belongs to the genus Macrobrachium of family Palaemonidae. Among the commercially important freshwater prawns of India the most important cultivable species at present is the giant freshwater prawn Macrobrachium rosenbergii due to its superior cultivable attributes such as fast growth rate, large size, wide range of salinity tolerance, hardiness and compatibly to grow with carps. The species enjoys good consumer preference and domestic as well as export market demand. Freshwater prawns are cultured in freshwater or low saline brackish water (salinity <10 ppt) and can be cultured either alone (monoculture) or in combination with fishes such as carps, tilapia and milkfish (polyculture). Freshwater prawn culture can be carried out in earthen ponds, cement cisterns, in pens or in cages. However, most of the operations are being carried out in earthen ponds.

1. To which family freshwater prawn belong?

 a) Arthropoda b) Crustacea

 c) Palaemonidae d) Malacostraca

 2. Scientifc name of freshwater prawn is .........

 a) Penaeus monodon b) Scylla serrata

 c) Macrobrachium rosenbergii d) Metapenaeus monoceros

 3. Freshwater prawn is also known as .........

 a) Giant river prawn b) Scampi

 c) Banana prawn d) Both a & b

 4. Identification features of freshwater prawn are .........

 a) Presence of grey and longitudinal streaks of light and dark colour

 b) The largest pereiopod of adult male is dark blue.

 c) The eggs of berried female are yellow in colour

 d) All of these

1. Web Based Services

People can reach the web, which is short for "World Wide Web," which is a huge network of papers and tools that are all linked together. The web, which was created by Tim Berners-Lee in the late 1980s, changed the way people around the world share and receives information.

The growth of technology has had an impact not only on the formats and sources of the information, but also on the manner in which library services are provided and the locations where they are provided. A portion of libraries and the resources they provide have been relocated to the digital realm created by the Internet. The web works on a client-server model: web computers (clients) ask web servers for web pages and other data and then show them. There are connections between the text, pictures, movies, and apps that make up these tools.

Web development is based on key tools like HTML (Hypertext Markup Language), CSS (Cascading Style Sheets), and JavaScript, which make it possible to make web pages that look good and let you interact with them. Over time, improvements like flexible web design (which makes sure that websites work on all devices) and web APIs (which let different software systems share data) have made the web experience even better.

Extension workers are required to visit farm homes as a fundamental aspect of effective Extension work. These visits are essential for building trust and securing the cooperation and friendship of farm families, especially in areas where the value of educational programs is not fully understood, where group meetings are uncommon, or where cultural norms prevent women from meeting publicly. As an Extension worker, reaching all members of the community involves employing various methods. While individual visits may not reach as many people as other approaches, there are occasions where only a personal visit will suffice. Words can be misunderstood, but a friendly smile, genuine empathy, or practical assistance are universally understood. It is crucial for Extension workers to be liked by families, as this acceptance is necessary for the families to accept their recommendations. Establishing rapport takes time; understanding local customs and observing social niceties during visits can significantly impact their success. For example, sharing tea with a family can be a critical gesture.

1. Introduction
The term “plastic” is derived from the Greek word “Plasticos" whichmeans to be capable of molding into various sizes and shapes. Daily usedsynthetic plastics are categorized based on their physical and chemicalproperties, such as thermoplastic and thermosetting. (i) Thermoplastics: Astheir names suggest, thermoplastic polymers melt and turn into a plastic orrubbery state when exposed to heat. They have various numbers of polymericchains and are the most widely used plastics in day-to-day life. Examples ofthermoplastics are Polystyrene(PS), Polyvinylchloride (PVC), Polypropylene(PP), Polyethylene Terephthalate (PET), Polyethylene (PE) etc. (Massy,2017), (Sharma et al., 2017). (ii) Thermosetting polymers: As the namesuggests, they set or harden permanently when heated (Massy, 2017). Due totheirstrong crosslinked structure, they are non-recyclable polymers. Examplesinclude Polyurethanes(PUR), Phenol formaldehyde, Melamine formaldehyde,and Polyester Resin (PR) (Sharma et al., 2017). Different polymers and theirapplications are illustrated in Fig. 1.

Introduction

No revolution occurs without disruption. Its effects break down traditions, behavior, relationships, ways of thinking, and methods of education. (Barreiro, 2021) throughout history, from the hunter-gatherer era to the present day humanity has experienced multiple industrial revolutions impacting both social and economic domains. The latest industrial revolution, which has ushered in digital transformation and enhanced productivity at reduced costs, introduces a new lifestyle distinct from our traditional daily routines. It also aims to foster a more sustainable overall environment. (Javaid et al., 2022).

The rise of Industry 4.0 is fueled by both rapid technological advancements and various social and economic factors. Central technologies propelling Industry 4.0 include wearable, augmented reality, simulation, autonomous vehicles and robots, additive manufacturing, distributed ledger systems like block chain, big data analytic, mobile computing, and cloud computing. These technologies influence and facilitate the development of new and innovative business models. (Industry 4.0 Implications for Higher Education Institutions 2019)

The advent of Industry 4.0, characterized by the integration of advanced technologies has revolutionized various sectors, including higher education. As these technologies reshape industries, they also redefine educational paradigms, creating a more interconnected, efficient, and personalized learning environment. This transformation, known as Education 4.0, responds to the demands of the Fourth Industrial Revolution by preparing students with the skills and knowledge necessary to thrive in an increasingly digital and automated world.

Introduction
Wastewater management has emerged as a critical environmental concern, posing severe threats to ecosystems, human health, and animal life due to  inadequate treatment and disposal systems. Various technologies have been employed to manage wastewater effectively, among which biological treatment techniques have gained widespread acceptance. These methods are  favoured for their affordability, operational simplicity, minimal reliance on chemicals, lower energy consumption, and eco-friendly characteristics (Smith and Brown, 2020). Among biological treatment processes, both aerobic and anaerobic methods are extensively utilized, with specific focus on Upflow Anaerobic Sludge Blanket (UASB), Expanded Granular Sludge Blanket (EGSB), and UASB-aerobic digester reactors. Comparative analyses of these approaches highlight their treatment efficiencies, advantages, drawbacks, and operational constraints. Additionally, the integration of aerobic and anaerobic treatment systems, particularly the UASB-aerobic digester combination, has shown promising results in optimizing wastewater treatment (Jones et al., 2021).
In many underdeveloped and developing regions, inadequate wastewater management has led to severe sanitation challenges, exacerbating health risks and facilitating the spread of waterborne diseases. The lack of proper wastewater treatment, combined with deficient healthcare infrastructure and inadequate housing, further aggravates the situation. Wastewater typically contains high concentrations of biodegradable pollutants such as Total Suspended Solids (TSS), Biochemical Oxygen Demand (BOD), and Chemical Oxygen Demand (COD), which, if discharged untreated, contribute significantly to environmental degradation (Lee and Wang, 2019). Both organic and inorganic contaminants originating from domestic, industrial, and agricultural sources play a major role in water pollution. In regions suffering from water scarcity, the reuse of treated wastewater is an essential strategy for water conservation and sustainability (WHO, 2022).

3.1 Introduction

Women are the backbone of the family. God gave her all the power to give birth, raise the children, do all the household chores, and balance the professional work. But we forget that women are also human beings, not machines. They can’t work 24x7 they also need rest as well as proper nutrition. Women in ancient India were very powerful in terms of decision-making, political opinion, education, etc, but later on, the situation of women deteriorated. In the middle era, the situation was at its worst: women were being treated badly by the men in their own families. Currently, the situation is turning better. Women are being treated equally now times but it is not the best scenario we can have. There is still much scope in this field where equal wedges and a good environment can be provided to the women.

During the last 21 years, the scientists and scholars of the M.S. Swaminathan Research Foundation (MSSRF) have been working on the design and implementation of projects which could have a large extrapolation domain in respect of imparting a pro-nature, pro-poor, pro-women and pro-sustainable livelihood orientation to technology development and dissemination. I would like to write about a few of the MSSRF initiatives, which have now become state, national and global programmes.

Mahila Kisan Sashaktikaran Pariyojana: Strengthening the role of women in agriculture

MSSRF initiated the Mahila Kisan Sashaktikaran Pariyojana in the Vidarbha region of Maharashtra in 2007 for empowering women farmers, including the widows of farmers who had committed suicide, in areas related to enhancing the productivity, profitability and sustainability of small-scale rain-fed farming. The empowerment measures incorporated access to technology, credit, inputs and market.

9.7.1. Function

The front axle is the unit on which the front wheel is mounted. This wheel is an idler wheel by which the tractor is steered in various directions. The function is to assist the steering and support the tractor. It is a rigid tubular or I-section steel construction, pivoted at the centre. The pivot pin is housed on bushings on a support bolted to the front of the engine. As the track width of a tractor is adjustable, the front axles have provision to be extended on both sides. The tubular front axles have telescopic arrangement for extension, whereas the I-section type of front axles are three-piece axles.

Maize or corn (Zea mays L.), which was first domesticated in Mexico, is the most widely grown cereal crop in the world. Maize is consumed as feed and food, and also has myriads industrial applications, including the production of bioethanol and starch. Due to its highest genetic potential, production and productivity among the cereal crops, maize is called as “Queen of the Cereals”. Noble Laureate Dr. Norman E. Borlaug, the Father of Green revolution stated 15 years ago that “The past two decades saw the revolution in rice and wheat, the next few decades will be known as maize era”. In India, maize is the third largest grain crop and its production and demand is increasing rapidly. In India, it is primarily a non-food cereal crop, with only 10% being consumed as direct human food, while the rest being used for feed, industrial and export purposes. The effective deployment of various frontier technologies viz. genetic engineering, doubled haploids, molecular markers, phenomics etc.has greatly helped in raising productivity of maize crop worldwide in the last decade.The present chapter provides an overview on role of frontier technologies in maize crop improvement.

India, a predominantly agrarian nation, relies heavily on agriculture for livelihoods. Over the decades, agricultural policies have focused on increasing the supply and accessibility of staple grains. This strategy has successfully reduced hunger and transformed the country’s food system (Pingali et al., 2019). At the forefront of this transformation stands Punjab that played a pivotal role in moving India from a food scarcity to self-sufficiency. Punjab’s agricultural success is globally recognized, driven by the rapid technology adoption, enabled through synergy of infrastructure, incentives and most critically, information (Singh & Kohli, 1997). Among these drivers, dissemination through agricultural extension proved instrumental in ensuring that innovations such as high-yielding variety (HYV) seeds, irrigation facilities and market incentives reached farmers effectively. While various factors contributed to Punjab’s growth, but agricultural extension remains a cornerstone, translating research breakthroughs into fieldlevel impact. Punjab Agricultural University (PAU) has been a key catalyst in this journey. while serving as a premier institution in agricultural research, education, and extension. PAU’s extension system has evolved in response to shifting agricultural needs, environmental challenges, and technological advancements. Beginning in 1960s, PAU led the Green Revolution by introducing HYVs and modern agricultural inputs through a dedicated, farmer-focused extension network (Gulati et al., 2018). In its early decades, PAU’s extension services emphasized increasing productivity through mechanization and the use of fertilizers and pesticides. Initiatives such as Kisan Melas (Farmers’ Fairs) and Kisan Clubs’ facilitated knowledge transfer to rural communities, fostering large-scale adoption of new technologies. However, by the late 1980s, concerns such as soil degradation and pest resistance prompted a strategic pivot. The PAU approach shifted from purely productivity-focused methods to more sustainable and diversified practices such as Integrated Nutrient Management (INM), integrated weed management (IWM) Integrated Pest Management (IPM), water conservation, etc. Today, PAU continues to modernize its extension practices through participatory, farmer-centric approaches. The current emphasis lies in promoting innovation, entrepreneurship, adaptability and sustainability through a blend of traditional outreach and modern ICT tools. Notable innovative approaches include e-extension services via mobile apps and online portals, real-time advisory systems through SMS and digital platforms, hybrid training modules