eChapters

Introduction

Research and development (R&D) is one of the main components to tackle the problems persisting or likely to take place in future. Priority areas of research are depending upon the overall situation and requirements of the problem under consideration. Under these broad areas, topic of interest for research may be varying at different geographical level from household to nation or global. If it is addressing vast geographical area like states, nations, regions and/or global, there may be many individuals/groups/institutions are working over similar type of problems. With the growing communication facilities and rising fund requirements, collaborative research programmes emerged to provide effective and economic solution to the problem with respect to time, money and manpower etc. Earlier, it was limited to high priority areas like defense and long run planning of the nations or kingdom at nation or state level. Declining environmental conditions and biological diversity became global concern in seventh decade of twentieth century. To tackle this problem, The United Nations Conference on the Human Environment (Stockholm) resolved to establish the United Nations Environment Programme (UNEP) in 1972. Further, to speed up the progress, global community through world leaders had identified two burning priority areas during conference United Nations (U N) Environmental Programme Rio de Janeiro, Brazil in 1992, well known as Earth Summit. One of those two priority areas was Biological Diversity. To address the issues at global levels, The Convention on Biological Diversity was signed by party nations, which was later ratified by the 187 countries. India is also one of the signatory nations and need to fulfill some time bound commitments. Moreover, later, importance of biodiversity and germplasm has been underlined by different forum at regional and global level for its present and future values in the life of human kind. Countries and various geographical regions are now becoming more and more aware about its judicious exploitation, conservation and sustained higher output over period of time.

Electrophoresis is an analytical method frequently used in molecular biology and medicine. It is applied for the separation and characterization of proteins, nucleic acids and subcellular-sized particles like viruses and small organelles. Its principle is that the charged particles of a sample migrate in an applied electrical field. An electric current is passed through a medium containing the molecules, and each molecule travels at a different rate depending on its electrical charge, size and shape. Separation by electrophoresis is based on these differences. In electrophoresis, agarose and acrylamide gels are used for electrophoresis of nucleic acids and proteins.

What is Gel Electrophoresis?

A gel is a solid form. The term electrophoresis describes the migration of charged particle under the influence of an electric field. Electro refers to the energy of electricity. Phoresis, from the Greek verb phoros, means “to carry across.” Thus, gel electrophoresis refers to the technique in which molecules are forced across a span of gel, motivated by an electrical current. Activated electrodes at either end of the gel provide the driving force. A molecule’s properties determine how rapidly an electric field can move the molecule through a gelatinous medium.

10.1 Learning Objectives
After reading this chapter, you will be able to:
i) Understand the concept and types of hypotheses in hypothesis testing;
ii) Differentiate between one-tailed and two-tailed tests and recognize their applications;
iii) Grasp the implications of Type I and Type II errors in hypothesis testing;
iv) Explore the significance level and its connection to confidence limits;
v) Understand the concept of degrees of freedom in hypothesis testing;
vi) Familiarize yourself with the stepwise approach to hypothesis testing for effective application.

10.2 Introduction

In this chapter, we explore the fundamental concept of hypothesis testing, a cornerstone in statistical analysis. We begin by exploring the nature of hypotheses and their types, distinguishing between one-tailed and two-tailed tests and understanding their respective applications. We then explain the intricacies of Type I and Type II errors, crucial pitfalls to avoid in hypothesis testing. Additionally, we probe into the significance level, its relationship to confidence limits, and its role in determining the strength of our conclusions. Furthermore, we explain the concept of degrees of freedom, an essential factor in hypothesis testing that affects the reliability of our results. Finally, we guide you through a stepwise approach to hypothesis testing, ensuring a comprehensive understanding and effective application of these concepts in practical scenarios. Through this journey, you will not only grasp the mechanics but also appreciate the profound impact of hypothesis testing in drawing meaningful conclusions from data in our data-driven society.

Litchi (Litchi chinensis Sonn.), a subtropical perennial fruit tree, belongs to family Sapindaceae and is indigenous to China. Its cultivation spread to the neighbouring countries of the south-east Asian region and other countries. The fruit of litchi is small, heart-shaped, conical or spherical in shape and pinkish red to bright-red in colour, with varying colour intensity in different cultivars. The pulp of fruit is crispy and grape-like in texture, very succulent and aromatic and is generally characterized by a sweet, acid, juicy and soft taste. Because of these reasons, litchi is highly prized in its fresh form as well as in different processed formulations. The total litchi production is about 24,00,000 tonnes from the net cultivated are of about 8,00,000 ha. The main litchi-producing countries are China, India and Thailand, whereas it is commercially grown also in many other countries. Of the total world litchi production, China and India contribute about 90%. Although litchi plants are long-lived, even then new plantings of this fruit are being done regularly, ensuring the continuous growth of production at the global level. In India, where this fruit can be grown in off season also in the southern states of Karnataka, Tamil Nadu and Kerala, the litchi production is about 7,37,200 tonnes from an area of 99,170 ha (Chandrakala et al., 2023).
 

Tropical root and tuber crops are important staples for food security for about a fifth of the world population. Tropical root and tuber crops like cassava, sweet potato, yams and aroids [taro and elephant foot yam] come under tropical root and tuber crops. Sweet potato, yams and colocasia are processed mainly as fresh vegetable, whereas 75% of the cassava in Africa, 75-80% in Asia and 65% in Latin America are processed either as fermented foods and feeds or as food additives such as starch, starch based sweeteners (glucose and fructose syrups), organic acids (acetic, lactic, glutamic and gluconic), mono-sodium glutamate and microbial polysaccharides (xanthan, pullulan, scleroglucan, etc).

In India, only two species viz. Capsicum annuum and Capsicum frutescens are known and most of the cultivated varieties belong to the species Capsicum annum. Insect pests and diseases are among the major constraints to enhancing production and productivity of chilli. There are a number of diseases, bacterial and viral infections that can attack chilli plants. They can sometimes be difficult to identify as they can have similar symptoms. The limited availability of commercial cultivars, resistant to pests and diseases especially in hilly areas affects its productivity. This makes more important for the farmers to rely on proper management practices to control pests and diseases.

This chapter attempts to brief the effect of insect pest and diseases on production of chilli. It also describes the proper management techniques of insect pests and diseases in chilli, highlighting the economics of their management. The chapter covers the description of insect pests of chilli like Aphid, Red Spider Mite, Slugs and Snails, Caterpillars, Capsid Bugs and Thrips and diseases like Bacterial Wilt, Anthracnose, Cercospora Leaf Spot, Damping off and Leaf Curl.

Introduction

India is a major producer, exporter and consumer of chilli. Chilli (Capsicum annuum) belongs to the genus Capsicum under Solanaceae family. It is also called as hot pepper, cayenne pepper, sweet pepper etc. Chilli besides imparting pungency and red colour to the dishes is a rich source of vitamin A, C and E and assists in digestion. Recently, Russian scientists have identified Vitamin P in green chilli which is considered to be important as it protects from secondary irradiation injury. The pungency in chilli is due to an alkaloid capsaicin which has high medicinal value. Capsaicin has many medicinal properties, especially as an anti-cancerous agent and instant pain reliever. It also prevents heart diseases by dilating blood vessels. The Chilli plant is a white flowered, dark green or purple leaved plant that grows upto 1.5 m in height. The major chilli exporting countries with their percentage share in world total exports are India (25 %), China (24 %), Spain (17 %), Mexico (8 %), Pakistan (7.2 %), Morocco (7 %) and Turkey (4.5 %).

Introduction
Domestication is the process of adapting wild plant species for human cultivation and consumption and it has played a pivotal role in shaping modern agriculture. Since the dawn of civilization, humans have selectively bred plants to enhance desirable traits, such as increased yield, improved taste, and resistance to pests and environmental stresses. Through domestication, early agriculturalists inadvertently altered the genetic makeup of wild progenitors, favouring traits that aligned with their needs and preferences. This process involved selecting and propagating plants with desired characteristics over successive generations gradually transforming once-unruly wild species into more docile, productive and palatable varieties. The domestication of crop plants was a gradual and ongoing process, often spanning thousands of years. It required keen observation experimentation, and an inherent understanding of plant biology and genetics even before these concepts were formally understood. This patient and persistent effort by our ancestors paved the way for the development of diverse and nutritious food sources, enabling the establishment of sedentary communities and the rise of complex civilizations. While modern breeding techniques have advanced significantly, the fundamental principles of domestication remain a cornerstone of crop improvement efforts. By understanding the historical significance and underlying mechanisms of domestication, we can better appreciate the invaluable contributions of our ancestors and continue to build upon their legacy, ensuring a sustainable and diverse food supply for generations to come.
The current state of food systems is being challenged due to the heavy reliance on a limited number of resource-intensive staple crops. The emphasis on maximizing yield and the consequent reduction in diversity over the course of domestication history has led to the development of contemporary crops and agricultural practices that are environmentally unsustainable, susceptible to the impacts of climate change, lacking in essential nutrients, and contributing to social inequalities. For many years, researchers have advocated for diversification as a viable solution to tackle the issues surrounding global food security. In this context, we present a vision for a new phase of crop domestication, which aims to expand the range of crop diversity. This approach involves active engagement and benefits for three key components of domestication: a) the cultivated crops themselves, b) the surrounding ecosystems, and c) the human populations depending on these crops. We delve into the potential of current tools and technologies to initiate a revival of diversity within existing crops, enhance the value of underutilized crop varieties, and introduce new crops into cultivation. This multipronged strategy aims to reinforce genetic diversity, promote resilient agroecosystems, and establish a more varied and robust food system. The successful execution of this renewed phase of domestication hinges on a bold commitment from researchers, funders, and policymakers to invest in both fundamental and practical research efforts. As we navigate the challenges of the anthropocene epoch, characterized by significant human impact on the environment, the process of domestication offers a pathway to construct more diverse and adaptable food systems that can better serve humanity’s needs.

Introduction

Potato (Solanum tuberosum) is a widely cultivated and essential staple crop globally, known for its versatile culinary applications and nutritional value. With the exception of Kerala, potatoes are grown in practically every state in India under a variety of climatic circumstances. During the brief winter months, which span from October to March, 82% of potatoes are grown in plains. In West Bengal, potatoes are the most widely grown crop after grains (Chakraborty, 2012). Originating from the Andes region of South America, potatoes have become a crucial part of diets worldwide, providing carbohydrates, vitamins, and minerals. With a rich history dating back to ancient civilizations, including the Irish potato famine, this crop has played a significant role in shaping agricultural practices and food security.

Introduction
Angiosperms produce attractive and intricately structured flowers, within which their reproductive process occurs. In flowering plants, as in other plant groups, a diploid spore-producing generation (sporophyte) alternates with a haploid gamete-producing generation (gametophyte). The flower contains specialized structures, such as the stamen (or androecium) and the pistil (or gynoecium), where the respective male and female gametophytes are formed. The pollen grain serves as the male gametophyte, while the embryo sac serves as the female gametophyte. These gametophytes are responsible for producing sperm and egg cells, respectively facilitating their union during fertilization. The pollen grain contains the male gametophyte in angiosperms or gymnosperms. Conversely, spores function as a resting and dispersal phase for cryptogams (Moore et al., 1991). The study of pollen grains (produced by seed plants, angiosperms and gymnosperms) and spores (produced by pteridophytes, bryophytes, algae and fungi) is known as palynology. This discipline examines the structure and formation of pollen grains and spores, their dispersal and their preservation under various environmental conditions. Palynology serves as a valuable research tool for understanding many aspects of plant and animal metabolism. Moreover, a deeper understanding of pollen can aid plant breeders in enhancing efforts to improve global food and fiber supplies. In the anthers of higher plants, neither the developing pollen nor the surrounding tissues exhibit autonomous growth and development. Instead, the developing anther functions as a system where the gametophytic and sporophytic generations of the plant communicate, coordinate and co-participate in development. This chapter aims to understand the various aspects of gene activity that occur during pollen development.

Abstract

Micronutrient (vitamin and mineral) deficiencies affect more than 2 billion people worldwide and are especially prevalent in developing countries including India. Conditions due to vitamin mineral deficiencies account for 7.3 percent of the global disease burden.Iron deficiency (anemia), vitamin A and iodine deficiency disorders continue to be a grim public health problem. Thus, elimination of vitamin and mineral deficiencies is essential to improve the overall health of the masses as well as to sustain economic growth and national development. Food fortification is an effective intervention to deliver vitamin and mineral rich foods to large populations. In addition to the prevention of vitamin and mineral deficiencies, food fortification is an effective measure to raise vitamin and mineral levels by moving the population towards consuming recommended dietary allowances for most micronutrients-making it a long–term and a sustainable strategy. India is the largest producer of milk in the world with 146.3 million tonnes and the consumption rate of milk and milk products by all age groups of people are high.Therefore, fortification of milk with mineral and vitamins could be a safe and effective strategy to fight these micronutrient deficiencies.

Introduction
Fossil fuels are finite and as population growth drives energy demands, the need for renewable energy solutions has become paramount. Among the many ways of producing biofuels, the use of algae has emerged as a promising source. Algae particularly, microalgae are photosynthetic organisms that can convert CO2 into fuel compounds. This innovative way of using microalgae to produce biofuels can address both sustainability as well as the depletion of fossil fuels.
1.1 Microalgae as energy source
Microalgae can be used over conventional biomass as they have several advantages. One of the biggest advantages is their rapid growth rate. They are easy to grow and multiply very quickly. Other benefits include high oil yield and their adaptability to different growth conditions. They also use CO2 to grow making them carbon neutral. Conventional biomass obtained from sugar bagasse or corn needs a lot of resources for a small yield, such as land, water, and electricity whereas microalgae do not require these high investment costs or land. However, the advantages of microalgae do not stop with biofuel production. Researchers have established the use of microalgae across various industries. Commonly, spirulina has been used as a source of protein surpassing protein content per 100 g compared to other protein-rich sources. Similarly, various other microalgae have been used for extracting vitamins, and essential fatty acids, and some are also used as feed for livestock. In recent years, microalgae have also been used in cosmetics for their antioxidant, collagen-boosting properties that can lead to anti-aging benefits. In the supplement industry, microalgae have been used in research as alternatives to mainstream ingredients. Microalgae also have other environmental benefits as they can be used to treat wastewater due to their ability to degrade pollutants and excess nutrients that concentrate in water bodies. With these benefits at hand, they can also be used as an excellent input for the production of biofuels (Razzak et al, 2022; Ramaraj et al, 2015). The figure below shows the various applications of microalgae such as the production of proteins, carbohydrates, biofuels, materials production, etc.

Abstract
 
India shares 16% of the world population, while its land is only 2% of the total geographical area of the world. Naturally, the pressure on the land is often beyond its carrying capacity. Therefore, the productive lands, especially the farmlands in the India are in the constant process of various degrees of degradation and are fast turning into wastelands. At present, approximately 68.35 million hectare area of the land is lying as wastelands in India. Out of these lands, approximately 50% lands are such non-forest lands, which can be made fertile again if treated properly. It was unprotected non-forestlands, which suffered the maximum degradation mainly due to the tremendous biotic pressure on it. In the last 50 years it is India's lush green village forests and woodlots have been deforested to the maximum. 

Introduction

The general recommendation for intake of fruits and vegetables is at least 400 g/ person as recommended by World Health Organization (WHO), which amounts to five servings of 80 g/day or about 146 g/person/annum. For vegetables, the recommended intake requirement is at least 300 g per day per individual. This 300 g requirement must include 50 g leafy vegetables, 50 g roots and tubers and 200 g other vegetables (Kapur, 2016). Current annual vegetable production including potato, based on recent three years (2015-16, 2016-17, 2017-18) average is around 177 million tonnes from 10 million ha giving a productivity of 17.7 tonnes/ha. With this average and stagnating production and assuming Indian population at 133 crores (1.33 billion) and 25% post-harvest losses, per capita per day availability of vegetables comes to 273 g leaving a gap of 27 g between the recommended dose and the availability of vegetables/head/day in India. By 2050, the projected population of India is 1.5 billion and to meet the requirement of this population, vegetable production will have be 220 million tonnes, of course making allowance of 25% post-harvest losses. Considering all kind of constraints on the available land area of vegetables, the vegetable area will be around 10 million ha and consequently, major gain of production will have to come from productivity increase from current level of about 18 to 22 tonnes/ha and this seems to be a gigantic task as during last several years, the productivity of vegetables in India has been between 17 and 18 tonnes/ha, an indication that perhaps productivity has reached to a plateau. This situation will call fall massive interventions on technological fronts in terms of innovative products with focus on better hybrids with higher yields, and resistance to biotic and abiotic stresses coupled with horizontal scaling up of modern production technologies. This chapter looks into the modern production technologies as applicable to vegetable crops in India to boost the productivity further from current 18 tonnes/ha to at least 22 tonnes/ha.

15.1 Introduction

It is an important vegetable grown since long and used as fresh vegetable. It is an important legume vegetable rich in proteins. The crop is usually grown for its green pods which contains immature seed and are cooked as vegetable, alone or mixed with others. It prefers cool weather and it does not grow well during intense heat of the summer season, especially the wrinkled seeded cultivars which are tender, sweet and of much better quality than the smooth cultivars. 

An ice cream plant is a highly interconnected operation in which quality, food safety, productivity, maintenance, cost, cold-chain performance, and business results influence one another. This chapter presents plant audits as management tools rather than simple compliance exercises. It provides a structured monthly audit system covering raw materials, production, quality assurance, hygiene, maintenance, productivity, cost control, warehouse operations, cold chain, documentation, and KPI performance. Department-wise scoring enables management to compare performance, identify weaknesses, prioritize corrective actions, and track improvement. The chapter also discusses common audit mistakes and best practices. A disciplined audit system strengthens accountability, reduces losses, improves operational control, and supports continuous improvement.

INTRODUCTION

Total forest cover in India is 70.17 million ha or 21.34% of the total geographical area according to the State of Forest Report 2015. Of this, 8.59 million ha (2.61%) is very dense forest, 31.54 million ha (9.59%) is moderately dense and rest of 30.04 million ha (9.14%) is open. The trees outside forest (TOF) are estimated to cover 9.26 million ha area which constitutes about 2.82% of the total geographical area of the country.  Thus the total forest and tree cover of the country is 24.16% of the total geographical area. The estimates of total growing stock of forest and TOF are 4.195 billion m3 and 1.573 billion m3, respectively. The total growing stock of wood in the county is estimated to be at 5.678 billion m3 which implies an average growing stock of 72.63 m3 ha-1 in 79.42 million ha of forest and tree cover (FSI 2016).

The accumulation of nitrogen under non-leguminous and cereal cropping systems seem to be generally universal at least in the tropics. Free-living heterotrophic nitrogen fixing bacteria are very widely distributed taxonomically, geographically and ecologically. But only a few genera and species are known to possess the potential for high amount of nitrogen fixation. Azospirillum has shown high nitrogen fixation attributes. The association of Spirillum lipoperum (Azospirillum brasilense) and Digitaria decumbens was reported by Dobereiner and Day (1974).

I. Spirillum

The description of members of genus Spirillum was available as early as 1670s by Leeuwenhoek (Dobell 1960 and Muller 1773). But the genus Spirillurn was created in 1832 by distinguishing it from phototrophic spirilla which were placed in a separate genus Thiospirillum (Ehrenberg, 1838). The subsequent developments of the taxonomy of the chemo- heterotrophic spirilla which generated knowledge of their characteristics, were extensively reviewed by Giesberger (1936) and Williams (1959 a, b).

Shelf life is a critical measure of ice cream quality and depends on formulation, processing, hardening, packaging, storage, and distribution conditions. This chapter explains the concept of shelf life and follows the movement of ice cream through the storage and distribution chain. It identifies temperature fluctuation and thermal abuse as major causes of quality deterioration, particularly through ice recrystallization and structural damage. The chapter examines hardening, formulation stability, cold-chain controls, shelf-life KPIs, and formal shelf-life studies. Common defects and their causes are also discussed. By understanding how temperature and handling influence product stability over time, manufacturers can establish better controls and protect texture, flavour, appearance, packaging integrity, and consumer acceptance.

The application of agricultural chemicals for control of insects, pests, fungi, weeds and plant diseases plays an important role in crop production. Before 1980 few chemicals were known for control of plant pests.  The chemical pesticides have played an important role in the rapid advancement of agricultural production. Chemical pesticides are getting popular due to non availability of labours especially for weeding operation during kharif season. The chemical pesticides and herbicides are applied with the help of different types of sprayers and dusters.

1. Introduction

A cooperative agricultural extension service has been implemented in Japan by both national and prefectural governments to support small-scale farmers who emerged as a result of land reform after the Second World War (Yamada, 2008).  In recent years, however, the scope and level of agricultural extension advisors’ activities have become more sophisticated and diversified because of technological progress and a wider range of activities, from production and selling to promoting relevant political measures.  On the other hand, the number of agricultural extension advisors has been decreasing for several years because of a cut in government expenditure for the cooperative extension service.  For this reason, information and communication technology (ICT) is needed to promote advanced and efficient extension activities (Yamada, 2010).