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Phenolic compounds constitute a major class of plant secondary metabolites that are widely distributed in the Plant Kingdom. Plant phenolics are biosynthesized through the shikimate/phenylpropanoid pathway leading to different compound classes. They may occur in their natural sources in free forms, as glycosylated or acylated derivatives, and as oligomeric and polymerized structures, such as hydrolyzable and condensed tannins, phlorotannins or lignins. They may also be found linked to plant matrix components such as cell walls, carbohydrates or proteins. It should be indicated that although the terms “plant phenolics” and “polyphenols” are indistinguishably used by some authors, they are not synonymous.

Alkaloids are a class of basic, naturally occurring organic compounds that contain at least one nitrogen atom. Alkaloids showed quite diverse medicinal properties (Rates, 2001). Many of them possess local anesthetic properties, but their practical use is limited for clinical purposes (Hesse, 2002). Morphine is one of the most known alkaloids isolated from Papaver somniferum (opium poppy), which had been used and still is for medical purposes. This alkaloid is a powerful narcotic which is used for the relief of pain, but its usefulness is limited because of its addictive properties.

The following plants have been worked out in the laboratory of Prof. N. Raaman: Cynodon dactylon, Artemisia pallens, Leptadenia reticulata, Clausena dentata, Tylophora indica, Aristolochia bracteolata, A. indica, Cissus quadrangularis, Hypericum mysorense. and Solanum trilobatum. Out of some of the data of these plants have been included in the previous chapters. In this chapter, compound isolation (CD1) from Clausena dentata (worked by Dr. R. Kamaraj) and characterization of the compound are given in detail.

Phytochemicals are the individual chemicals from which the plants are made. Phytochemical is simply a word that means plant chemicals. Plants have the ability to synthesize mixtures of structurally diverse bioactive compounds with multiple and mutually potential therapeutic effects. The plants have the capacity of manufacturing the secondary products. Phytochemicals with antioxidant properties tend to be brightly colored because they contain chromophores, ie, a series of alternating single-bonded and double-bonded carbons. Isoprene is often the building block of such units. The darkest green vegetables contain the most chlorophyll, and vegetables with the most chlorophyll require the most antioxidants.

After isolation and purification of the compound, it has to be identified. It is necessary to find out the class of the compound first and then to find out the specific compound within the class. The class of compound is usually determined from the colour tests, solubility, Rf properties and UV spectral characteristics of the compound. Further identification within the class depends on measuring physical properties and spectral measurements and then comparing these data with those in the literature.

For qualitative and quantitative analysis of phytochemicals, chromatography can be used. Gas chromatography is a powerful means of performing qualitative and quantitative measurements of complex mixtures of volatile substances, which are vaporizable without decomposition.Gas Chromatography-Mass Spectrometry (GCMS) system is a widely used instrument for qualitative and quantitative analysis of solid, liquid, and gaseous samples. Samples are converted into gaseous ions and then separated on the basis of their mass-tocharge ratio. 

All separation procedures depend mainly on some physical characteristics of the compounds. It is relatively easy to separate compounds that have significantly different physical characteristics by simple techniques such as solvent extraction. If the various compounds are similar to each other in their molecular size, any slight difference in any one of their physical properties is exploited to achieve separation. 

The different qualitative chemical tests can be performed for establishing profile of given extract for its chemical composition. The following tests may be performed on extracts to detect various phytoconstituents present in them.

Extraction is a process whereby the desired constituents of a plant are removed using a solvent. The primary ways for extraction of organic molecules of interest to biologists and medical investigators involve breaking open the cells. Cell rupturing is carried out in a variety of ways. The method used depends on the type of tissue used. For plant cells grown in cell suspension culture or for plant callus tissue, a French press or a sonicator can be used to break open the cell.

Phytochemicals from various parts of a plant or tree such as the leaves, the flowers, the seeds, the barks, the roots and the shrubs can be isolated. Once one comes in from collecting plants in the field, it is good idea to freeze them immediately in a freezer at – 20°C or a commercial freezer held at – 80°C (Kaufman et al., 1999). According to Harborne (1984, 1998), fresh plant tissues should be used for phytochemical analysis and the material should be plunged into boiling alcohol within minutes of its collection. Alternatively, plants may be dried before extraction.

Dietary supplements from plant sources are sometimes referred to as phytopharmaceuticals. They are produced from fresh, dried or otherwise preserved plants or parts of plants. The active ingredients are usually not completely isolated but rather are obtained along with other naturally occurring components of the plant. These other components are often believed to influence the efficacy of the active ingredient. Sometimes the active ingredients are concentrated, and undesirable substances such as chlorophyll, tannins, or resins, are removed.

Plants are used as medicine since time immemorial. One of the area of Ethnobotany, Ethnopharmacology is considered as the scientific evaluation of traditional medicinal plants (Cotton, 1996). Cox (1994) has suggested that the ethno-directed sampling is most likely to succeed in identifying drugs used in the treatment of gastrointestinal, inflammatory and dermatological complaints. The Plant kingdom is a virtual goldmine of potential drug targets and other active molecules awaiting to be discovered. It has been estimated that only 10 - 15 per cent of the 7,50,000 existing species of higher plants have been surveyed for biologically active compounds.

1. Which of the following states has nutrient use of < 100 kg/ ha?

(a) Madhya Pradesh                (b) Karnataka

(c) West Bengal                       (d) Uttar Pradesh

2. Which of the following is the leading state in maize production

(a) Bihar                                   (b) Uttar Pradesh

(c) Punjab                                (d) Gujarat

Cochan W.G. (1946). Relative Accuracy of Systematic and Stratified Random Sampling for a Certain Class of Populations. Ann. Math. Statist, 17, 164- 177.

Cochan, W.G. (1977). Sampling Technique, 3rd edition, Wiley Eastern Ltd., New Delhi.

Q.1 In applying varying probability sampling for information of population total of X, it is required to know the values of selection probabilities Pi (i = 1, 2, 3,..., N).

Q.2 The technique of “Double Sampling” was first time formulated by Jayman, (1938) in connection of collecting information on strata sizes in stratified random sampling scheme.

Q.1 The most fundamental assumption to be followed by random variable for using the standard method (parametric statistic) or parametric inference is that the random variable follows normal distribution.

Q.2 The standard parametric techniques are not useful for estimating parameters and testing hypothesis about them.

Q.1 In addition to the assumption of ANOVA, the other point which is given due consideration by the statistician is “The stability of variance.”

Q.2 In any investigation, if it is possible to incorporate the “randomisation procedure” then it is customary to rely on “ Randomisation” to break up any “correlation among experimental errors”

Q.1 In random effect model, the treatments are taken as a random sample of treatments drawn from a large group of T treatments.

Q.2 In varietal trials for plant breeding or genetics, the appropriate statistical model is not the random effect model.

Q.1 Analysis of covariance is an extension of ANOVA.

Q.2 In an ANCOVA technique, observations are taken on more than one variable from each experimental unit.

Q.1 The replicated experiment designed to compare a number of parent plants w. r. to some attribute under study is known as “Progeny row trial?

Q.1 Let there be two factors A and B with p and q levels respectively which are applied to the larger strip by dividing the experimental material into p rows and q columns. The factors A and B are applied at random to these rows and columns respectively. Such a layout is known as strip plot design.

Q.1 If two type ot factors, say (A & B) have to be studied in the same experiment where A requires larger plot size and B requires smaller plot size and structure of the experiment is that of plots within blocks and blocks within replication then such layout is known as split plot design.

Q.1 Factorial experiment is not a design.

Q.2 In factorial experiment, all possible combinations of two or more than two factors are not considered

Q.1 We can apply the change of origin or change of scale or change of origin and scale both to the original data in case of applying ANOVA technique to make the analysis easy and speedy.

Q.2 The conclusions drawn from ANOVA based on transformed data or original data are different.

Q.1 According to prof. R. A. Fisher “ANOVA” is not the separation of variance assignable to one group of causes from the variance assignable to another group of causes.

Q.2 The basic purpose of ANOVA technique is to test the homogeneity (equality) of several means.

Q.1 An experiment is a means of getting an answer to the question which arises in the mind of an experimenter (reseacher).

Q.2 An experiment may be planned for not to compare the yield of several varieties of wheat.

Q.1 A sampling procedure in which the units are selected with probability proportional to their some size measure, is known as sampling procedure with probability proportional to size (P.P.S.) Notations: Let there be a population of N units say Yi , (i = 1,2, ......... N), YN with their selection probabilities Pi (i=1, 2........... , N). Here Pi ? Pj V i ? J. = 1, 2, .............. N. Let yi , (i = 1, 2, ...................n) be the sample drawn from the population with probability of selection pi (i = 1, 2 -, n).

Q.1 Cluster sampling restricts the spread of sample over the population which results generally in increased sampling variance of estimators under considerataion.

Q.2 In adopting cluster sampling, few selected clusters are enumerated partially.

Q.1 Informations available on an auxiliary variable X which is highly correlated with the study variable y, can be utilized in improving the sampling design for the study variable y.

Q.2 Information on auxiliary variable X can be used for stratification of population for the study character y.

Q.1 Systematic random sampling or systematic sampling, both are different sampling schemes.

Q.2 The pattern usually followed in selecting a systematic sample is a simple pattern involving a constant spacing of units in the sample.

Q.1 In the theory of cluster sampling, if the population is divisible into smallest, distinct and identifiable units then this smallest unit is known as elementary unit (element) of the population.

Q.2 A group of elementary units in context of sample survey theory is known as cluster.

Q.1 If the sampling units of the population are of heterogeneous nature then stratified random sampling scheme is suitable for sample survey to be conducted.

Q.2 In stratatified random sampling scheme, the strata are homogeneous within themselves but heterogeneous between themselves.

Q.1 SRSWOR scheme is better (more efficient) them SRSWR scheme.

Q.2 The sampling variance of sample mean under SRSWOR scheme is more than that in SRSWR scheme.

.3 The sample variance s2 is an unbiased estimate of population M.S. (S2 ) in case of SRSWOR scheme.

In this chapter some preliminary terminologies have been provided which are very much useful and frequently used in dealing with the theory of sample surveys and its application.

Mango is considered to be the king of fruit. India is the largest producer and exporter of mango in the world. Mango possess unique nutritional and medicinal qualities apart from being a rich source of vitamins A & C, besides its attractive form and appearance, delicious taste and appetizing flavor, the ripe mango fruit according to nutritional experts is also highly invigorating, fattening, laxative and diuretic.

Immediately after withdrawal of monsoon (September-October), offseasonal trunk spraying with Malathion 50 EC @ 15 ml in 10 lit of water suppresses the adult hoppers from multiplying further and hinders their oviposition and also kills the newly hatched nymphs.

Food grain production has experienced a considerable jump from about 51 million tonne during 1950 to 291.95 million tonne in 2019-20 as per second advance estimates. The production of horticulture products has doubled over the past quarter century and the value of global trade in horticulture crops now exceeds that of cereals. For the first time, horticulture production surpassed food production in India during 2013-14 and continues to excel currently.

The horticultural produce includes fruits, vegetables, flowers, plantation crops and spices. However, fruits and vegetables are the most consumed and important commodity among them. Morphologically and physiologically, the fruits and vegetables are highly variable and may come from a root, stem, leaf, immature or fully mature and ripe fruits. They have variable shelf life and require different suitable conditions during marketing.

Fruits and vegetables account for nearly 90% of total horticulture production in the country. India is the second largest producer of fruits and vegetables in the world and the leader in several horticultural crops namely Mango, Banana, Papaya, Cashewnut, Arecanut, Potato and Okra. However, the nature of horticultural crops being such that it’s not easy to make assessment of their production. These crops, especially vegetables are grown in small plots, fields or in the backyard of the houses;

The world ‘‘Unfruitfulness’’ means a state or character of being unfruitful or barrenness. It is a vital constraint in so many fruit crops leading to a great loss to the farming community. In contrary, ‘Fruitfulness’ is the state where a fruit plant is not only having the ability of flowering and fruiting, but also carry on these fruits up to maturity. The inability of the plant to do so is known as ‘unfruitfulness’ or ‘barrenness’. 

Juvenility is referred to the phase of plant growth following germination from seed during which flowering does not occur and the bud meristem is not competent to respond to seasonal environmental inductive cues, and hence plant remains vegetative. The changes from juvenile to mature characteristics are well known as phase changes phenomenon. Plants are unable to initiate flowering after germination and have to undergo in a juvenile developmental phase.

Fertilizer is a substance of natural or synthetic origin, applied to soils or to plant tissues (usually leaves) for the supply of one or more nutrients essential to the plant. It is classified in two types, inorganic fertilizers and organic fertilizers. Both the fertilizers play major role to the crops if they are properly and appropriately applied.

Irrigation is one facet of the inputs which need to be carefully managed so that profits are maximized. Irrigation plays a major role in any agriculture crop production. One should be aware of different types of irrigation adapted in orchards or crop cultivation to become a successful grower. Irrigation is very important component of crop cultivation as sufficient moisture must be maintained in the soil for obtaining the optimum growth and good quality yield. The aim of irrigating a plant should be to wet the entire root zone without allowing any wastage of water beyond the root zone.

The high density planting (HDP) in fruit crops is one of the new concepts of increasing the productivity without affecting the quality of fruits. The concept of high density planting was pioneered in temperate fruits in Europe at the end of 1960, since then there is rise in establishment of commercial high density orchards throughout the world. Initially HDP was followed mostly in temperate fruits e.g., apple.

The fruit trees are grown for optimum production of quality fruits that fulfill our requirement. Therefore, proper care and management of trees is mandatory to manipulate plant growth and plant environment. Training and pruning are important operations that provide not only the proper framework to the tree but also the favourable environment leading to desired plant growth and reduction in disease and pest attack.

Layout means the way by which something is arranged or laid out. The purpose of designing a layout for an rchard is to assign the actual position for trees/ plantations, roads, water channels, buildings etc. The layout should be planned very carefully and implemented properly to facilitate proper care and maintenance of an orchard.

Plant Propagation is a technique through which multiplication or production of new individuals take place from selected propagules.

A nursery is a place, where seedling, saplings, trees, shrubs, and other plant materials are grown and maintained until they are placed in a permanent place.

A seed (in some plants, referred to as a kernel) is a small embryonic plant enclosed in a covering called the seed coat, usually with some stored food. It is the product of the ripened ovule of gymnosperm and angiosperm plants which occurs after fertilization and some growth within the mother plant. 

The Horticultural crops are majorly classified on the basis of its consumption and uses as fruits, vegetables, flowers, spices, medicinal, aromatic and plantation crops. Moreover, these crops have been further classified into various groups on the basis of their habitat, growth pattern, edapho-climatic requirements and uses