eChapters

Introduction
Maize (Zea mays L.) is the second most widely crop grown globally after wheat, with 205 million hectares under cultivation. The area cultivated under maize is steadily increasing and expected to overtake that of wheat by 2030 (Erenstein et al., 2022). With production exceeding 1.2 billion metric tons, maize surpasses the production of other highly cultivated cereals like wheat and rice mainly due to its higher yields (Erenstein et al., 2022). Maize is cultivated in the Americas, Africa, and Asia, for multiple end-uses (Nuss and Tanumihardjo, 2010). It is a staple food in several countries across Africa and Latin America, and in a few countries in Asia, with an overall contribution of 15-20% of daily calorific needs (Shiferaw et al., 2011).
The global maize yield tripled from the 1960s with the average yield rising from 2 tons/ha to the current 5.8 tons/ha, mainly due to the adoption of highyielding hybrid cultivars and complementary input use (Erenstein et al., 2022). Maize is naturally an outcrossing species and inbreeding is unnatural. When maize is subjected to inbreeding and the resulting inbred lines from genetically distant backgrounds are crossed, hybrid vigor or heterosis is realized (Springer and Stupar, 2007). Hence, the development of the homozygous inbred lines is a very important step in hybrid maize breeding.
In the well-evolved maize breeding programs, the germplasm is well characterized into different heterotic groups where the hybrids developed by crossing inbred lines from different heterotic groups exhibit high heterosis (Ertiro et al., 2017a; Sang et al., 2022). For the development of inbred lines, maize breeders develop F1 populations by crossing two inbred lines within the same heterotic group (Prigge et al., 2011; Sang et al., 2022). Inbred lines are developed from the F1 population by recurrent self-pollination for 6-8 generations; this can take up to 3-4 years or even more, depending on the availability of off-season nurseries for generation advancement. The resulting inbred lines are highly homozygous (~98-99%), but not 100% homozygous. The doubled haploid (DH) technology emerged as a viable alternative to the time-consuming and resourceintensive recurrent selfing method to produce 100% homozygous inbred lines in just two crop seasons (Prasanna et al., 2012; Chaikam et al., 2019a).
In addition to significantly reducing the time taken to develop homozygous lines, the use of DH lines in maize breeding offers several other benefits: a) As DH lines are completely homozygous at all loci, they are stable and do not show further inbreeding depression; b) DH lines are also best suited for evaluating distinctness, uniformity and stability (DUS) criteria, thereby better facilitating plant variety registration and intellectual property protection (Röber et al., 2005); c) The use of DH lines facilitates selections on completely homozygous lines which is more reliable and effective compared to selections on segregating families with different levels of heterozygosity (Chaikam et al., 2019a); d) Use of DH lines also simplifies breeding operations like planting, managing the nurseries, seed shipments, record keeping, etc. as selfing nurseries spread over several generations are replaced by just two generations for DH line development (Prasanna, 2012; Röber et al.,

The history of evolution reveals that life on Earth has gradually gone enriching itself by adding more and more varieties to it. A variety of organisms as of today exhilaratingly blossoms in the biosphere. The organisms are divided into three broad categories based on their distinctive characteristics, viz. plants, animals, and microorganisms. The other broad categories all the organisms on Earth are placed in are the five kingdoms, viz. Protista, Monera, Fungi, Plantae and Animalia. These kingdoms encompass living organisms based on their cell type (prokaryotic or eukaryotic), cell number (unicellular or multicellular), genetic recombination type (binary fission, asexual or sexual type), or feeding behavior or mode of nourishment (autotrophic or heterotrophic).

Immunity

Immunity is the resistance of body against extraneous etiological factors of disease, which is afforded by the interaction of chemical, humoral and cellular reactions in body. This is an integral part of the body without, which one cannot think of life. During the process of evolution, nature has provided this defense mechanism in the bodies of all living creatures particularly of higher animals and man, that protects them from physical, chemical and biological threats. It can be classified as natural or paraspecific and acquired or specific immunity.

Natural/paraspecific immunity

There are some species which are resistant to particular diseases due to presence of natural resistance against them e.g. horse, pig, cat are resistant to canine distemper virus; dogs are resistant to feline panleucopenia virus, chickens are resistant to anthrax. Even within species, there is natural resistance that protects some individuals while others are susceptible e.g. Indian deshi cattle Zebu (Bos indicus) is quite resistant to piroplasmosis in comparison to Bos taurus. Besides, there are the mechanisms or barriers in body provided by nature.

Introduction

Microbes (bacteria, archea and eukarya) are single celled organisms that rule the planet. Microbes occupy every possible niche, from the high atmosphere to the deep ocean, from hot springs to artic ice, from hot deserts to deep forests including many regions that cannot yet be accessed for study. The biosphere contains between 1030 and 1031 microbial genomes, which is about 2-3 times more than the number of plant and animal cells combined (67). In fact, studies have shown that microbes associated with the human body are about ten times more than the number of human cells (5).  Microbes play a critical role in the life cycle of all living organism and environment in general.  Some microbes cause disease, but the most of them are either innocuous or play a role in physiology and cellular response including immune response, digestion and vitamin production of various organisms.

Endocrine glands are organs which secrete chemical substances called hormones into the blood stream. Hormones are substances produced by specific glands, secreted directly into the blood and transported to various organs and tissues where they exert their effects. The term hormone is derived from the Greek root meaning to excite. The organs/cells where there hormones bring about their actions are called target organs/cells. The target organs (hormone binding receptors) posses specific receptors for bringing about the action of these substances. The term neurosecretion signifies the production and reiose of hormones by nerve cells that have the cytological characteristics of secretory cells.

Several hormones are the product of nervous mechanisms hence the term neurosecretion is used and neurohumour for such substances.

Hormones act as a mode of transmitting information in the body in addition to the nervous system. Their mode of action is slow but more prolonged. Chemically the hormones are either proteins or steroids. Hormones are secreted in very small amount but their actions are profound. Early investigators were hampered by the non availability of suitable methods for their estimation. The availability of radioimmunoassay, radio-receptor assay, enzyme linked immunological assay have greatly facilitates researches in the field of endocrinology.

The mode of action of these hormones has been elucidated in several instances. Involvement of membrane permeability and cyclic AMP has been implicated in several cases.

Most of these hormones have been of therapeutic interest for several decades. The mode of administration of steroid hormones can be both oral or parenteral while protein hormones are active only when injected. Several of these hormones are used for increasing the production potential of meat animal, for improving the reproductive efficiency of domestic animal, hence are of direct economic importance to the farmers. Chemical characterization and their synthesis including their more potent analogues in several cases, have greatly facilitated their widespread application in the animal production and animal health strategies.

Endocrinology is the study of the structure and function of the endocrine glands and their secretory products, including the consequences of excessive or deficient production of these hormones.

1665 Robert Hooke had successfully invented the microscope. Because of this discovery, Robert Hooke was the first one to have a close look of a cell appears to be. His description of these cells was published in Micrographia. However, the cell walls observed by Hooke gave no indication of the nucleus and other organelles found in most living cells.

1674 Anton van Leeuwenhoek witnessed a live cell (plant) under a microscope.

1775 Antoine Lavoisier first proposed a mechanism for photosynthesis, a process wherein plants plants take in carbon dioxide and release oxygen. Lavoisier was also the first to investigate cell respiration in animals.

1777-83 Chemistry became dominated by the phlogiston theory, or the hypothetical principle of fire wherein all the combustible material was part composed. In this principle, burning (oxidation) was caused by liberating phlogiston, with ash as the dephlogistigated substance.

1836 The proponent of the cell theory in Biology, Theodore Schwann, proposed that the process of fermentation is solely limited to living yeast cells in 1836. Liebig did not agree to this and instead, he proposed another alternative theory of fermentation.

1856 Louis Pasteur opposed Liebig’s chemical theory. In his experiment, he showed that fermentation depends highly on the physiological functions that occur in bacteria and in living yeast cells. This work of Pasteur in 1856 received general recognition.

1860s The view on the chemistry of life highly different from the chemistry of nonliving things. During this period, the view is that the gelatinous and homogenous form of matter in organisms or more commonly known as the protoplasm carries out all the intracellular processes. These include respiration, biosynthesis of molecules, and the breakdown of matter.

Single strand conformation polymorphism (SSCP), or single strand chain polymorphism, is defined as conformational difference of single stranded nucleotide sequences of identical length as induced by differences in the sequences under certain experimental conditions. This property allows to distinguish the sequences by means of gel electrophoresis, which separates the different conformations.

Background

A single nucleotide change in a particular sequence, as seen in a double stranded DNA, cannot be distinguished by electrophoresis, because the physical properties of the double strands are almost identical for both alleles. Contrarily, after denaturation, single stranded DNA undergoes a 3- dimensional folding and may assume a unique conformational state based on its DNA sequence. The difference in shape between two single-stranded DNA strands with different sequences can cause them to migrate differently on an electrophoresis gel, even though the number of nucleotides is the same. Predictability, however, is a shortfall of SSCP - since conformational states are subject to many experimental conditions and sequence differences that cause minimal changes in strand conformation may not be detected.

4.1 Introduction

There are some mathematical tools that are applicable in solving the mathematical problems related to the subject matter on “Economic Theory”. Differential calculus is one of those mathematical tools. Hence the useful theory of differential calculus will be discussed in this chapter.

4.2 Differentiation

The mathematical term “Differentiation” is involved in finding the rate at which a variable quantity changes.

In the theory of “Differential Calculus” we are concerned fundamentally with the “Rate of Variation” in a function y = f(x) with respect to the change in other variable (Independent Variable X) on which the dependent variable ‘Y’ depends through a relation Y = f(x).

Soil supports diverse arthropod communities, including insects, mites, springtails, spiders, millipedes and centipedes. These organisms contribute to decomposition, nutrient cycling, soil formation, microbial regulation and food-web stability. Their activities improve soil fertility, structure and overall ecosystem functioning. However, soil arthropods are highly responsive to temperature, moisture, vegetation and other environmental factors. Global warming, altered rainfall, drought and changes in soil properties can modify their diversity, abundance, distribution and ecological interactions. Despite their importance, climate-change effects on soil arthropods remain comparatively understudied. This chapter examines their ecological roles, relationships with soil properties and responses to changing climatic conditions in agricultural ecosystems.

Food security is achieved when adequate food is available and accessible and satisfactorily used and utilized by all individuals to live a healthy and active life. But food and nutrition security is achieved when people have physical, economic and social access to food of sufficient quantity in terms of variety, diversity, nutrient content and safety to meet their dietary needs and food preferences for a healthy and active life coupled with sanitary environment, adequate health and education care. In order to achieve food and nutrition security there is a need for transitioning toward nutrition sensitive food system.

Introduction 
Before 1800, it was believed that organic compounds could only be synthesized by living organisms, not possible in the laboratory. Friedrich Wohler successfully synthesized the first organic compound urea in the laboratory. Recently, synthetic organic chemistry has been a well-established science to construct derivative or new compounds, which have been utilized in the fields of medicine, food, clothing, nutrition, polymers, plastic, highly durable materials, and high energy fuels, that are beneficial to humans, and society. To achieve faster organic transformations, many advancements have been made, as a result, catalysts have been taken into account, which provides an alternate faster path for the reaction by lowering the activation energy. The catalysts play a very crucial role in achieving organic transformations more efficiently, affordably, and with higher yields in less time. Additionally, the catalyst which is environment-friendly and easily recoverable after completion of the process, would be beneficial in both economic and ecological ways (Trost, 1991). The utilization of nanoparticles as catalysts in organic transformations is more advantageous over conventional catalysts because of lower catalyst loadings, and high performance of the reaction in heterogenous phase. The large surface area of nanoparticles is responsible for high reactivity towards organic transformations. Till now, many nano-dimension metals like gold (Au), silver (Ag), platinum (Pt), and palladium (Pd) efficiently utilized to catalyze organic transformations (Muskan et al., 2022). The ever-growing demand for productive and sustainable catalytic systems has driven the utilization of transition metal nanoparticles as catalysts in organic conversions (Pathak et al., 2024). Among these, copper-based nanoparticles have shown great potential as catalysts due to their large surface area and variable oxidation states, which enable them to function as both oxidizing and reducing agents, effectively replacing traditional palladium catalysts (Pathak et al., 2024). Copper-based nanoparticles having specific physicochemical properties, varying in size, porosity, surface/volume ratio, and shape can also be synthesized through different physical, chemical, and biogenic methodologies. Most physical and chemical methods are considered toxic, have huge energy demands, and have high-temperature demands. In contrast, biogenic methods are simple and non-toxic. The biogenic procedure can be done by using microbes, fungi, algae, plants, extracts, etc. This chapter provides insights into various aspects of the biogenic synthesis of copper-based nanoparticles using plants and multiple catalytic applications in recent organic transformations.

Artificial seeds have been widely used for micro-propagation of many plant species. Establishment of gene banks for ex situ conservation of plant germplasm in the form of field gene banks, seed gene banks, in vitro collections, and cryogenically preserved tissues is a common practice (Withers 1983; Rao, 2004; Borner, 2006). Alginate encapsulation provides a viable approach for in vitro germplasm conservation as it combines the advantages of clonal multiplication with those of seed propagation and storage (Standardi and Piccioni, 1998; Ara et al., 2000).

The main aim of artificial seeds is to obtain true to type plants to maintain the germplasm, but, however, during tissue culture, there is a chance of genetic aberration which is commonly known as somaclonal variations. Larkin and Scowcroft (1981) for the first time used the word ‘somaclonal variation’ to describe these variations as displayed among the plants derived from tissue culture. It is clearly understood that genomes modify themselves when exposed to unfamiliar conditions, and these modifications are best described as programmed loss of cellular control (Phillips et al., 1994).

Fixation of elemental nitrogen in the atmosphere by the microorganisms through a reductive process into ammonia is called biological nitrogen fixation (BNF). Molecular nitrogen (N₂) is the major component (approximately 80%) of the earth’s atmosphere. The element nitrogen is an essential part of many of the chemical compounds, such as proteins and nucleic acids, which are the basis of all life forms. However, N₂ cannot be used directly by biological systems to build the chemicals required for growth and reproduction. Before its incorporation into a living system, N₂ must first be combined with the element hydrogen. This process of reduction of N₂ commonly referred to as nitrogen fixation (N-fixation).

N₂is transformed into reactive N via lightning discharge (5 teragrams (Tg) N / year; 1 Tg equals 1012 g) and by biological N fixation (100 -140 Tg N /year ). Conversion of dinitrogen to ammonia in the industrial process requires temperatures of 400 - 500^o C and high pressures (Haber-Bosch process). Biological nitrogen fixation is much more important as it contributes to more than 70 % of the input in to the world’s soil and water nitrogen. It has been estimated that 100 to 175 million tones of nitrogen are fixed annually by the process of biological nitrogen fixation.

The process of N₂ fixation is mediated by the enzyme, called nitrogenase (which reduces N₂ to ammonia). The overall reaction is as follows.

Ethylene is a primary plant hormone involved in the senescence of plant tissues including flowers and leaves. In carnation, inhibition of the synthesis or action of ethylene delays the onset of senescence symptoms and lengthens the vase life of flowers. Leaves of cut chrysanthemum of some cultivars frequently become yellow, sometimes prior to onset of flower senescence, in response to exogenous ethylene. Generation of transgenic plants with suppressed production or action of ethylene is an excellent way to lengthen the longevity of these ornamental plants. This paper reviews our studies on generation and characterization of carnations with reduced ethylene production and chrysanthemum with reduced sensitivity to ethylene.

Ethylene is a primary plant hormone involved in the senescence of cut flowers of many ornamentals such as carnation, sweetpea and delphinium. It is produced on a large amount at a later stage of flower senescence. The increased ethylene production accelerates withering and dropping of petals. Inhibition of synthesis or action of ethylene delays the onset of senescence symptoms and extends the vase life of flowers.

• Neoplasm General

• Neoplasm in cattle and buffaloes

• Neoplasm in sheep and goat

• Neoplasm in equines, swine and camels

• Neoplasms in pet animals

• Neoplasms in poultry

• Neoplasms in wild animals

• Neoplasms in laboratory animals

1.1 Introduction
Concentrated milks, like evaporated milk and sweetened condensed milk, serve as practical substitutes for fresh milk in regions where access to it is limited or where transportation and storage are challenging. These products go through specific processing techniques to reduce their water content, thereby improving their shelf life and transportability. Evaporated milk is created by removing water through evaporation and then
sterilizing it to prevent microbial growth. This process results in a thicker consistency and a slightly caramelized flavor due to the heat treatment. On the other hand, sweetened condensed milk is made by adding a significant amount of sucrose to the milk and excluding oxygen, which inhibits microbial growth. This results in a thick, sweet product suitable for various culinary uses. These concentrated milk products are usually packaged in small cans, making them convenient for storage and transportation. However, they are sometimes diluted before consumption with water to resemble the taste and consistency of fresh milk. In regions where even concentrated milks are not easily accessible, alternatives like whole milk powder or recombined milk are used. These alternatives involve dehydrating milk into powder form or reconstituting milk components to create a liquid similar to fresh milk. Innovations in processing and packaging have led to the development of alternative uses for concentrated milks, expanding their versatility beyond traditional applications. These products continue to provide essential dairy
nutrition in areas where fresh milk is scarce or hard to obtain, offering a shelfstable solution to meet dietary needs.

Over the past 12,000 years Homo sapiens have moved from the hunter–gatherer life-style to settled agriculture to advanced civilization in urban society with improved quality of life. Livestock has played an important role as an instrument of bringing out this change. Farmers all over the world, attach great importance to livestock ownership as a source of nutrition, as a mechanism for capital accumulation, and concomitantly, as a symbol of wealth and status in the community. Just as in today’s society, money is used to exchange goods and services, livestock including goats used to play the same role in times gone by. Most farmers consider livestock as a important resource for income generation. Livestock sector plays a significant role in supplementing family income and generating gainful employment in the rural sector, particularly among the landless, marginal and small farmers and women, besides providing cheap nutritional food to millions of people. History indicates that livestock has been not only a resource, but also an instrument of dominance and a source of conflict in the society. Livestock especially small ruminants have often served as a means for exchange of goods and services and thus becoming increasingly important in the growth of agriculture in developing economies.

Livestock species which man domesticated for improving the quality of human life include goat, sheep, cattle, buffalo, pig, poultry, horses and camels. Among these goats were the first among livestock species domesticated by man. Goats are among the smallest domesticated ruminants and have served mankind longer than cattle or sheep. Goat is an important source of meat, milk, skin, fiber and manure since then. Traditionally goats have been an important source of income generation among small households. Goats have been used to buy salt, cloth and groceries in many communities. Goats provide a large part of the cash income for smallholder farmers. Goats are friendly animals; with proper attention they maintain good health and can be managed easily even by children.

Sailors and other explorers out on the high seas kept goats as a ready stock of meat & milk on long voyages. Captain Cook’s goat had already sailed around the world before it entered on its first voyage with Cook, and helped save everyone on board the ship from scurvy. Goats are some times kept as pet or companion animal. Their use as pet is common in European countries especially U.K. Some owners keep goats for purpose of goat shows.

A flood occurs when water exceeds the carrying capacity of a river, stream, drain, gully, or another natural or artificial channel and spreads over adjoining land. Its severity depends on water volume, depth, velocity, duration, timing, and the vulnerability of affected communities. Prolonged inundation can severely damage crops, property, infrastructure, water supplies, and human and animal life. Night-time floods may be particularly dangerous because warnings and evacuation become difficult. This chapter introduces flood occurrence and mechanisms, India’s and the world’s flood history, flood-prone regions, flood types and causes, major consequences, and the responsibilities of Indian flood-control and forecasting agencies.

Answer Inflammatory or Not

• If yes it is non-neoplastic or inflammatory
• If no, consider neoplastic conditions
• Certain non-neoplastic conditions may not show inflammatory cells (Haematoma, cystic fluid, epidermal/dermal cysts)
• Inflammation or non-neoplastic condition is characterized by a mixed population of cells including neutrophils, lymphocytes, plasma cells, eosinophils, macrophages, epithelioid cells and giant cells.
• Inflammatory reactions often result in hyperplasia of surrounding tissues.
• If not inflammatory, it may be neoplastic.

Introduction
Freezing is one of the oldest and most widely used methods of food preservation, which allows the preservation of taste, texture, and nutritional quality in foods better than any other methods. The freezing process is a combination of the beneficial effects of low temperatures at which micro-organisms cannot grow, chemical reactions are reduced, and cellular metabolic reactions are delayed. Freezing is generally referred to as a unit operation in which the temperature of food is reduced below the freezing point and a proportion of water undergoes a change in state to form ice crystals. Immobilization of water to ice and the resulting concentration of dissolved solutes in unfrozen water cause a lowering of water activity of the food. Thus, reduction in water activity and use of low temperatures coupled with some pre-treatments like blanching is the basis for food preservation by freezing. Fruits such as strawberries, oranges, raspberries, black currants, and vegetables like green peas, green beans, sweet corn, spinach, carrots, sprouts, and potatoes are examples of commercially frozen fruits and vegetables.
The frozen food market is one of the largest and most dynamic sectors o the food industry. Despite considerable competition between the frozen food industry and other sectors, large quantities of frozen foods are being consumed all over the world. The frozen vegetable industry mostly grew after the development of scientific methods for blanching and processing in the 1940s. The commercialization history of frozen fruits is older than frozen vegetables. The commercial freezing of small fruits and berries began in the eastern part ofthe United States in about 1905.