eChapters

Role of Biotechnological Approaches

In our fore-going chapters, we have studied variety of plant products such as alkaloids, glycosides, volatile or essential oils, gums and mucilage and oleoresins with varied role to play to the plants as well as to human.Knowledge of plants and knowledge of healing have been closely linked. It is well established that industries have many direct and indirect effects on human population. Increased stress is most evident at the same time health awareness goes parallel in the recent times. Most of the natural plant products are compounds biosynthetically derived from primary metabolites such as amino acids, carbohydrate metabolic intermediates and fatty acids. These secondary metabolites are the major sources of pharmaceuticals, food additives, fragrance and pesticides.

Application of biotechnology know how helps to synthesize the novel molecules and also could be beneficial to enhance the production of secondary metabolites. Classical definition of biotechnology states that it is “The use of living organisms or their products to enhance our lives and our environment.” The word Pharming is commonly use to describe genetically engineered plants or livestock to produce medically useful products –predominantly protein drugs. Plants or animals are genetically engineered to contain genes capable of producing a biologic or drug compound. Therapeutic proteins are designed to treat illness includes serum proteins, cytokines, growth regulators, anti coagulants, antbiotics, lysosomal enzymes and plant secondary metabolites.

Abstract

Biotechnology offers an opportunity to exploit the cell, tissue, organ or entire organism by growing them in vitro and to genetically manipulate them to obtain desired compounds. It plays a pivotal role in search for alternatives to production of desirable biomedical compounds. There are several approaches have been used to increase the yield of metabolites from cell cultures such as manipulation of nutrient medium, application of exogenous growth stimulators, chemical treatment, precursor feeding, elicitation, transformed cultures, metabolic engineering, micropropagation, bioreactor cultures and etc.

Abstract

India with population of 1.30 billion in which 47 per cent is engaged in agriculture earnestly needs efficiently use of biotechnology and genetic engineering for transforming agriculture sector. Biotechnological tools offer great opportunities for increasing agricultural production and can play an important role towards the attainment of production sustainability of crops. Biotechnological tools have the potential to release burgeoning pressure on agriculture and allied sectors; ecology and environment; plant, animal and microbial biodiversity; and allied resources such as land and water with improved and sustainable agricultural productivity which enhances food security. Agricultural biotechnology, including tissue culture, DNA-marker-assisted breeding and genetic engineering (GE) technology have potential to increase stress tolerant, selection efficiency, crop adaptation, crop productivity and food security in more efficiently manner through the development of improved varieties with increased yields, nutritional content and storage characteristics. Genetic engineering tools should continue to build capacity in this technology, giving priority to traits and crops that are of economic importance such as drought tolerance in maize, rice, beans; cold tolerance in rice; increased nutritional value and improved shelf life in fruits and vegetables. Crop varieties and livestock with improved characteristics have been developed using tools such as marker-assisted breeding and genetic engineering. Molecular pharming is one of the applications of genetically modified crops and livestock for the production of pharmaceutical proteins. Reproductive technologies are alternative ways for genetic improvement of livestock. The development of molecular diagnostics and vaccine technology as a tool improved the ability to detect and treat several diseases of livestock. The advancements in biotechnological tools such as protein engineering and metabolic engineering play a significant role in production of a large number of valuable and high quality industrial products.

Introduction Worldwide, 40% of a crop is lost to insect damage, particularly in developing countries. Some of the major insect pests belong to the Lepidoptera and Coleoptera, and their larval or grub stages cause major damage to economically important crops including tobacco, cotton, corn, sorghum, tomato, sunflower, pulses, wheat, and lucerne. Recently, some insecticides have been primarily used for agricultural pests. Over the years, most pesticides have led to insecticide resistance in major lepidopterans, a reduction in natural enemy populations, and harm to mammals and the environment.

10.1 INTRODUCTION

‘Tissue culture’ is commonly used as a blanket term to describe all types of plant cultures, viz., callus, cell, protoplast, anther, meristem, embryo and organ cultures (George, 1993). It relies on the phenomenon of cell totipotency. The different techniques of culturing plant tissues may offer certain advantages over traditional methods of propagation.

Growing plants in vitro in a controlled environment, with in-depth knowledge of the culture conditions and the nature of the plant material, ensures effective clonal propagation of genetically superior genotypes of economically important plants. Micropropagation has become an important part of the commercial propagation of many plants (George and Sherrington, 1983; and Zimmerman et al., 1986) because of its advantages as a multiplication system (Pierik, 1997 and Razdan, 2003). Several techniques for in vitro plant propagation have been devised, including the induction of axillary and adventitious shoots, the culture of isolated meristems and plant regeneration by organogenesis and/or somatic embryogenesis (Williams and Maheswaran, 1986; and Gautheret, 1985).

Tissue Culture is becoming as an alternative means to vegetative propagation of plants. In vitro growing plants are usually free from bacterial and fungal diseases. Virus eradication and maintenance of plants in virus free stage can also be rapidly achieved in cultures (Sharma, 2016).

Introduction

Plants are often subjected to unfavorable environmental conditions – abiotic factors, causing abiotic stresses - that play a major role in determining productivity of crop yields but also the differential distribution of the plants species across different types of environment. Some examples of abiotic stresses that a plant may face include decreased water availability, extreme temperatures (heating or freezing), decreased availability of soil nutrients and/or excess of toxic ions, excess of light and increased hardiness of drying soil that hamper roots growth. The ability of plants to adapt and/or acclimate to different environments is directly or indirectly related with the plasticity and resilience of photosynthesis, in combination with other processes, determining plant growth and development, namely reproduction. A remarkable feature of plant adaptation to abiotic stresses is the activation of multiple responses involving complex gene interactions and crosstalk with many molecular pathways.

Pineapple is a short-stemmed herbaceous plant that necessitates a significant amount of planting material. For example, depending on the intensity of planting and the cultivar used, one hectare of cultivation needs 35,000 to 70,000 planting parts. Realizing the need for quick multiplication, Ma (1968) devised a method for quick pineapple multiplication using the decapitation technique. Later a technique for rapid in vitro propagation has been developed. Since then, numerous reports on crop micro-propagation using tissue culture techniques have been published. On supplemented MS medium, different types of explants, such as leaf base, shoot base, excised lateral buds and tips from crowns, young-leaf segments, and excised leaf buds, have been used to regenerate plants. Plantlets raised in this manner thrived in the field. In vitro mutation may also be induced using tissue culture techniques. Shoot tips from in vitro grown plantlets may be treated with chemical mutagens (EMS) or irradiated with gamma rays to produce mutants with superior traits such as spineless leaves, larger fruits, improved pulp consistency, or disease resistance. Similarly, somaclonal variants can be screened out early to improve f ield results.

Introduction

The primary problem facing humanity at the start of the new millennium is to provide sufficient sustenance for every person living on this continent, given the rapidly growing populations of the majority of Asian countries. As the amount of land per person continues to decline, several strategies are being used to boost agricultural output from these finite resources in order to satisfy the growing demand of the population. But maintaining adequate soil health levels to support healthy plant development is the fundamental requirement for maintaining food production from these shrinking land areas. Questions concerning the over-reliance on mineral fertilizers to maintain the health and, consequently, the productivity of this continent’s arable soils are frequently raised by the recent trend of consistently decreasing the use efficiency of mineral fertilizers under highly productive systems coupled with issues of gradual deterioration of soil health due to indiscriminate use of fertilizers. It is widely acknowledged that mineral fertilizers are limited to providing plant nutrients to soils; they are not able to address other physical, chemical, or biological aspects of soil health. Conversely, organic materials are far more beneficial in this regard. In light of this, the idea of an integrated plant nutrition system that incorporates both organic and inorganic nutrient sources has been developed and is gaining traction (Chattopadhyay, 2005). Because most tropical and subtropical nations’ arable soils have low levels of organic matter due to high temperatures and increased microbial activity, the integration of mineral fertilizers and organic manures is particularly important for many Asian countries (Gaur, 2006).

Introduction

Wool which obtained primarily from sheep is an essential textile fiber that is used across the world. Whether for clothing, canvas work, carpets, or even upholstery, wool is a vital resource supporting many industries and occupations. This natural fiber has many benefits, such as being biodegradable, easy to clean and even having a natural structure that traps air which is excellent for heat retention. The annual wool production is 2 million tones. China ranks first in terms of global wool production. With a production of more than 333,624 tonnes of wool in 2020, China accounts for approximately 19% of the global wool clip. Australia produces 16% of global wool and is by far the largest wool exporter in the world. There has been a constant increase in the demand of wool products worldwide. This has made scientists to try to improve livestock and livestock associated derivatives. The conventional system along with the recent biotechnological interventions will be helpful for enhancing wool quality and production.

1. Introduction

 Banana is the developing world’s fourth most important food crop (after rice, wheat and maize) accounting for 70 million metric tons per annum (FAO stat, 2007 faostat.fao.org/site/567/Desktop Default.aspx?). Banana is also the second highest fruit crop in the world, representing a wide range of varieties of cooking and dessert types, providing a staple, nutritious inexpensive food for millions of people. The word “banana” is a general term embracing a number of species or hybrids in the genus Musa of the family Musaceae known simply as the banana family. Two Musa species namely Musa acuminata and Musa balbisiana, out of the 28 described were first noted in 1865 by Kurz as being the origin of most of the commercial clones (Stover and Simmonds 1987). Cultivated clones are parthenocarpic, mostly sterile with starchy fruits. Indeed, wild types are very fertile; their fruits are full of seeds with little starch, and are seldom fit for human consumption. Its abundance, varietal diversity, availability round-the-year and low cost make banana an important commodity for the religious occasions in many tropical countries. The vegetable bananas, generally grouped under ‘Plantains’ are larger, more angular than dessert type and are generally intended for cooking unripe, although ripened ones are quite like dissert bananas.

Introduction

The horticulture sector has been a driving force in stimulating a healthy growth trend in Indian agriculture. This sector contributes around 28 per cent of the GDP from about 13.08 per cent of the area and 37 percent of the total exports of the agricultural commodities (Rather et al., 2013)  India is currently producing 257.2 million tonnes of horticulture produce from an area of 23 million ha. Over the last decade, the area under horticulture grew by about 3.8% per annum but production rose by 7.6% per annum. The higher growth rate in horticulture was brought about by improvement in productivity of horticulture crops, which increased by about 28% between 2001-02 and 2011-12. (Anonymous, 2012)

1. INTRODUCTION

Food processing involves the use of wide range of unit operations and technologies to transform the raw, bulky and perishable food into more shelf-stable, palatable and marketable food products. Food processing is one of the most effective solution to reduce the wastages, improves the quality and safety aspects of food products, increase the food availability and marketability. Fruits and vegetables are considered as an integral part of any dietary system as these are rich sources of vitamins, minerals and dietary fibres. India is the second largest producer of fruits and vegetables after Brazil and China which contributes 10 % of world fruit production and 14 % of world vegetable production. Fruits and vegetables are more prone to spoilage as compared to cereals due to their nature and composition. The spoilage can be occurr at the time of harvesting, handling transportation, storage, marketing and processing that results in lot of wastage in the form of seeds, pulps, peels etc. Efficient management of these wastes can help in preserving vital nutrients of fruits and vegetables, and bringing down the cost of production of processed foods, besides minimizing pollution hazards. In India, processing of fruits and vegetables is extremely low as compared to the developed countries.

Biotechnology can help in the bio-processing of fruits and vegetables for the production of novel products having better sensory and nutritional qualities, improved shelf life. Bio-processes involve the use of different micro-organisms and enzymes along with modern biotechnological techniques like genetic engineering.

Small size grains namely millets are produced by extraneous grasses and the word millet is derived from ‘‘mille’ a French word synonymous to thousand indicating handful of millet grains in a handful measure contains thousand grains (Taylor and Emmambux, 2008). Small millets and pseudo cereals belong to Gramineae family and were major ancient foods of Asian and African continents while Western countries used them as only bird feed.

9.1 INTRODUCTION

India is the second largest producer of fruit crops with annual production of 68.4 million metric tonnes from an area of 6.1million ha (Anon 2010). The national horticulture mission commenced in 2005 had fixed a target to double the horticultural production by 2011-12. According to the available production data of horticultural crops, it seems that we are very close to achieve our target. But, if the loss caused by biotic and abiotic stresses is accounted, a sound conclusion can be drawn that still there is a huge scope for productivity improvement in fruit crops.

Among biotic factors bacteria, fungi, nematodes, viruses and insect pests, diseases and other pests are widely recognized as the biggest threat to the sustainable production of fruit crops. Every fruit crop is infected with one or other pathogen/pest, which causes a significant reduction in yield. The disease alone both in field and storage accounts for 25-30 per cent losses (Rangaswami and Mahadevan 2002). Along with diseases, insect-pests also cause reduction in the yield. About 600 insect and mite pests have been reported in temperate fruits alone in India (Sharma and Singh 2006).

Beside biotic factors, abiotic factors (salt, drought, low and high temperature) also limit the potential yield of fruit crops. It is estimated that nearly 10 million ha is affected by salinity/alkalinity in India (Singh 2006). Moreover, fruit cultivars are also to be modified for yield and traits of consumers preference e.g. seedlessness in grapes and citrus.

Genetic improvement can be achieved by following the conventional breeding approaches, when the gene of interest is present in related cultivars/species and the crop is sexually crossable. But due to long juvenility and genetically prevalent problems like incompatibility, sterility in most of the fruit crops and apomixis in citrus, mango and apple, conventional techniques seem to have limited role in the improvement of fruit crops.

Various biotechnological approaches either alone or in combination with conventional breeding approaches can provide a direct solution for the genetic improvement of fruit crops.

Biotechnology is one of the most exciting and revolutionary sciences of 21st century. According to Convention on Biological Diversity, biotechnology is defined as “any technological application that uses biological systems, living organisms, or derivatives thereof, to make or modify products or processes for specific use”. Biotechnology has proven its immense applications at every step of human life such as, health care, crop improvement, development of valuable products and abatement of environmental problems. Agricultural biotechnology is the area of biotechnology involving its applications to agriculture. Agricultural biotechnology has been practiced for a long time, as people have sought to improve agriculturally important plants/ organisms by selection and breeding.

Biochemical and biotechnological methods present eco-friendly and efficient pathways for converting biomass and organic waste into valuable resources, contributing to environmental protection and sustainable development. These methods rely on microbial and enzymatic processes to break down waste materials, resulting in the production of biofuels, chemicals, and other beneficial byproducts. In today’s pursuit of sustainability and circular economy principles, the concept of waste valorization—turning waste into valuable products—has gained significant traction. Biochemical and biotechnological approaches stand out due to their specificity, lower energy requirements, and minimal environmental impact. These techniques transform organic waste into bio-based products like biofuels, bioplastics, organic acids, enzymes, fertilizers, and animal feed. In contrast to thermochemical processes that operate at high temperatures and generate less selective outputs, biochemical processes function under milder conditions, using microbes and enzymes to achieve targeted conversions. These approaches reduce reliance on landfills and fossil resources while creating high-value products from underutilized biomass. A core aspect of biochemical valorization is the microbial breakdown of complex organic compounds such as carbohydrates, proteins, and fats commonly found in agricultural waste, food residues, and industrial effluents. This is accomplished by natural microbial communities or genetically enhanced strains with tailored metabolic capabilities. Anaerobic digestion exemplifies this process, where organic waste is decomposed without oxygen to yield biogas (mainly methane and carbon dioxide) and nutrient-rich digestate. The biogas can be harnessed for energy generation or as a transport fuel, while the digestate serves as a sustainable fertilizer, thereby supporting circular agricultural practices.

Summary

Idealistic theories of property are projected to offer general and explicit statements of the justification for deciding legal and moral questions about the status property claims. Debates over property rights in biotechnology were occasioned by specific legislative proposals such as the US Animal Patent Act of 1986, and by filing of patent applications for DNA sequences and processes in the early 1990s. While these debates make sporadic petition to philosophical theories of property, moral claims were entwined with questions about filing requirements, tests for worth, and the rules for licensing and defending patents. The comparative scarcity of discussion on intellectual property at that time dictated the general approach of the chapter: Review basic philosophical approaches to property rights, and speculate on how one might use these approaches in constructing an argument pertinent to agricultural biotechnology. This chapter deals with different approaches to intellectual property in the field of Plant Sciences especially the Biotechnology. It incorporate many different types of intellectual property and a variety of incommensurable values play a role in the justification of intellectual property.

Origin, History and Definition

The term biotechnology was coined by Karl Ereky, a Hungarain engineer, in 1919. The origin of biotechnology can be traced back to prehistoric time when armers and gardeners have been making use of hybridization and grafting among plants with a view to bring about an improvement in existing attributes. Using these approaches, several hundred varieties in ornamental plants were produced. Microorganisms were already used for processes like fermentation, formation of yoghurt and cheese from milk, vinegar from molasses, production of butanol and acetone from starch by Clostridium acetobutylicum or the production of antibiotics like penicillin from Penicillium notatum. Four defferent phases of biotechnological research were witnessed during the last century. In 1920, Chain Weizmann used Clostridium acetobutylicum to convert starch into butanol and acetone (during world war I, acetone was used as an essential component of explosives). Alexander Fleming in 1929 discovered penicillin from Penicillium notatum and wounded soldiers were given treatment using this antibiotic during world was II. In 1970s with the discovery of restriction enzymes, which led to the development of recombinant DNA, technology brought a revolution in the field of biology. The latest phase in the history of biotechnology is the study of structure and function of whole genome (DNA) and whole proteome (protein). This area of research is described as genomics and proteomics, respectively. Some more important activities in the field of biotechnology are given in Table1

In fact, biotechnology is the product of interaction between science of biology and technology. It is the technological exploitation and control of biological system. Biotechnology has wide range of its uses and accordingly Office of Technology Assessment described biotechnology as: (i) old biotechnology that includes fermentation, i.e. formation of vinegar from molasses and that of cheese and yoghurt from milk, production of antibiotics from certain fungi and process of baking and brewing and (ii) new biotechnology that includes the techniques of recombinant DNA and PCR polymerase chain reaction, cell culture and fusion and bioprocessing which became possible only through the researches in molecular biology. However, no efforts have been made to distinguish between old and new biotechnology. Keeping in view the importance of biotechnology, attempts have been made to define it. Following are some of the definitions of biotechnology, however, none of the definitions of biotechnology seems to be complete. The first definition seems to be comprehensive and wide.

Introduction

As per the reports of the United Nations Organization (UNO), the world population is expected to increase to about 9.7 billion by 2050 and to 10.4 billion by the mid-2080s. The increasing population will result in food scarcity and hunger across the world. To meet the demand for food to feed the people and meet the present aim of WHO of “Zero Hunger by 2030”, agriculture and livestock production needs to be increased. However, according to the ICAR Vision 2050 document, growth of the livestock sector is expected to be higher than the agriculture due to different factors like urbanization and changing food habits among the population residing in the urban area, lack of technology among the small and marginal farmers, reducing operational land, water scarcity, reduced irrigation infrastructure, climate change, etc. to name a few. But to achieve food security through the livestock sector, animal health and production of the animals need to be addressed.