eChapters

Abstract

Over the years, global climate change has negatively impacted the resources of western Himalayan region and the diversity of floral wealth has been endangered. The endangered livelihoods of agriculture based sector call for incorporating stress resistance by sustained effort that includes resort to infusion of new technologies. Biotechnology offers possible solutions to many of the intractable problems. Development and commercialization of transgenic crops have witnessed phenomenal growth in recent years. An array of traits viz. insect pest resistance, herbicide tolerance, abiotic stress tolerance, augmentation of nutritional qualities etc. have been targeted and successfully modified through plant genetic engineering. The global area of transgenic crops has seen a tremendous increase to attain 148 million hectares in 2010. Progress in understanding plant genome structure and function by high throughput characterization of genome, transcriptome, proteome and metabolome is providing new opportunities to deal with complex traits that result from interactions of a large number of genes. The other significant application of biotechnology in crop improvement has been “Marker Assisted Selection” (MAS). Development and integration of DNA based markers in the selection process has empowered the breeder to identify the desired genotypes without any interference of environmental effect or tissue specificity of expression. Cultivation of MAS derived crop varieties in the country bears testimony to the success of this technology.

1.    Introduction    496

2.    Strain Development Technologies    496

    2.1.    Screening of natural variants of microorganisms, 496
    2.2.    Genetic manipulation of microorganisms, 498
        2.2.1.    Random mutagenesis and selection, 498
        2.2.2.    Conjugation, 504
        2.2.3.    Transduction, 504
        2.2.4.    Transposition, 506
        2.2.5.      Hybridization, 507
        2.2.6.    Rare Mating, 509
        2.2.7.    Cytoduction, 510
        2.2.8.    Protoplast fusion, 511
        2.2.9.    Transformation, 511
        2.2.10.    Gene cloning and transformation (Recombinant DNA technology), 513

Any technique that uses living organisms or substances from these organisms to make or modify a product or to improve plant or animal or to develop microorganism for specific uses, can be termed biotechnology. Biotechnology involves microbes or cells derived from plants and animals for efficient production of useful products for mankind. The use of microbes for production of alcohol and penicillin, to name a few, is well known. However, in the last 25 years, several novel organisms, yielding useful products have been created by genetic engineering. Today, there is a wide application of biotechnology in human medicine, agriculture and animal husbandry in the area of diagnosis prophylaxis and therapy.

Aquaculture industry has undergone a sea change world over in the last three decades. The important needs of the industry are development of fast growing disease resistant fish and shellfish varieties, development of cheap and effective vaccines, disease diagnostics, cell lines sand probiotics. Efforts on these lines have already begun since 3-4 decades and results are highly promising. Some of the biotechnologies employed in aquaculture health management (Plate 15) are fish cell lines, monoclonal antibodies and nucleic acids for diagnostics sub unit and nucleic acid vaccines for prophylaxis, transgenes for disease resistance, gene editing in fishes and their pathogens and probiotics for bioremedial measures.

A. Cell Lines for Health Management

Cell lines are transformed cells that are adapted well to grow and multiply outside the host in in vitro culture systems. Cells lines can be developed from fast growing tissues of young fish. There are two methods of developing cell lines (1) Explant method, (2) Enzymatic method. Cell lines are grown in sterile plastic or glass containers containing appropriate medium having nutrients salts, antibiotics, buffers, serum etc. Minimum essential medium (MEM) is the common medium employed for fish cell line. Cell lines are developed, characterised and deposited in national and international repositories, which can be procured for use in research and industry.

Biotechnology can be defined as utilization of materials and methods for benefit of mankind by the utilization of microorganisms and biological agents. It is an area with a tremendous potential in solving basic problems of food, fibre, fuel and medicines in developing countries. According to the office of the Technology Assessment of the US Congress, biotechnology can be defined as any technique that uses living organisms to make or modify a product, to improve plants or animals or to develop micro organisms for specific uses. Flower industry has always increasing demand for new forms, colours and types of flowers. Biotechnology has made significant advances in the improvement of ornamental crops and development of new varieties has great potential to be utilized in future. Utilization of this technology can be done in floriculture under two broad subheads viz., tissue culture and genetic engineering.

TISSUE CULTURE

The major commercial utilization of tissue culture has been micropropagation, which is an alternative to vegetative propagation. Micropropagation permits true to type multiplication of a desired genotype using in vitro culture techniques. Micropropagation process can produce a large number of propagules in a relatively short time and space under controlled conditions. It has already revolutionized production process of several nurseries. The dream of multiplying disease free plants in required quantity on the specified time schedule in an industrial way has become true due to micropropagation. Some of the important floricultural crops that are successfully propagated in vitro in Indian laboratories are given below with their explant source:

Carnation: Leaf segments, internodes, petals

Gerbera: Leaf, petiole, flower stalk segments

Gladiolus: Inflorescence stalk, leaf sections

Orchids: Epidermal peelings, leaf segments, root tips

Rose: Internodal segments, petals, leaf segments, immature embryos, root segments

The main purpose of herbal drug technology is to convert botanical materials into medicines with a focus on standardization and quality control with proper integration of modern scientific techniques and traditional knowledge. The use of chromatographic techniques and marker compounds to standardize botanical preparations has limitations because of their variable sources and chemical complexity. DNA-based molecular markers have utility in fields like taxonomy, physiology, embryology, genetics etc. DNA based techniques have widely been used for authentication of plant species of medicinal importance. Pharmacognosy mainly addresses the quality related issues using routine botanical and organoleptic parameters of crude drugs, and chemoprofiling assisted characterization with chromatographic and spectroscopic techniques. The new pharmacognosy includes all the aspects of drug development and discovery where the biotechnology driven applications play an important role. Current focus on chemotype-driven fingerprinting and related techniques require integration with genotype-driven molecular techniques so that an optimal characterization of botanical materials is possible. This review provides a brief account of various DNA based technologies that are useful in genotyping and quick identification of botanicals with suitable examples.

Biotechnology is being harnessed in various aspects of the livestock industry to hasten breed development for improved animal health and welfare, enhanced reproduction, and improved nutritional quality and safety of animal-derived foods. Various biotechnology methods are used in improving the breeding stock of animals. These include artificial insemination (AI), embryo transfer (ET), in-vitro fertilization (IVF), sexing of semen and embryos, micromanipulation of oocytes/ embryos, transgenesis, somatic cell nuclear transfer and the emerging technology on genomics and marker-assisted selection (MAS). Artificial Insemination: One of the earliest perfected technology is artificial insemination (AI) where new breeds of animals are produced through the introduction of the male sperm from one superior male to the female reproductive tract without mating. AI reduces transmission of venereal disease, lessens the need of farms to maintain breeding males, facilitates more accurate recording of pedigrees, and minimizes the cost of introducing improved genetics. Various technologies have evolved that led to the efficient use of AI in developing desired livestock, including the methods of freezing semen or cryopreservation and sperm sexing.

Abstract

Tea [Camellia sinensis (L.) O. Kuntze], of family Theaceae, is an important cash crop  worldwide. It is an evergreen shrub cultivated in humid and sub-humid tropical, sub-tropical, and temperate regions of the world, and grows mainly on acidic soils. Due to limitation of expansion of tea cultivation in new land, import of low cost high quality tea, increasing vertical productivity is the best option of Indian tea Industry. The primary need of the tea industry today is to develop high yielding clones with better quality and stress tolerance potential. Conventional tea research has contributed appreciably to the genetic improvement of tea over the past several decades, but it is time-consuming and labor intensive.

11.14. Nanotechnology 11.14.1. Concept • Nanotechnology is science, engineering, and technology conducted at the nanoscale, which is about 1 to 100 nanometers. • Physicist Richard Feynman is considered as father of nanotechnology. The idea and concepts behind nanoscience and nanotechnology started with a talk entitled “There is Plenty of Room at the Bottom” by physicist Richard Feynman at an American Physical Society Meeting at the California Institute of Technology on December 29, 1959, long before the term nanotechnology was used. In his talk, Feynman described a process in which scientists would be able to manipulate and control individual atoms and molecules. Over a decade a later, in his explorations of ultra precision machining, Professor Norio Taniguchi coined the term nanotechnolog

Introduction
Biotechnology represents a transformative force in modern agriculture, offering innovative solutions to enhance crop production, improve resistance to diseases, and address the global challenges of food security and climate change. Chapter 9 delves into the multifaceted role of biotechnology, particularly in the realm of genetic innovation, and how it is reshaping the agricultural landscape. The chapter begins by exploring the various applications of biotechnology in crop improvement. This includes the development of genetically modified organisms (GMOs) and transgenic crops, which have been engineered to exhibit desirable traits such as increased yield, pest resistance, and enhanced nutritional content. These advancements have significantly contributed to the global food supply, particularly in regions facing harsh climatic conditions or limited agricultural resources. A detailed examination of genomic tools and techniques follows, highlighting the sophisticated methodologies employed in biotechnology. Techniques such as CRISPR -Cas9 gene editing, marker-assisted selection, and genomic sequencing are discussed, showcasing their pivotal role in accelerating the development of improved crop varieties. These tools have enabled scientists to manipulate genetic material with unprecedented precision, paving the way for more efficient and targeted crop breeding programs. The chapter also addresses the role of biotechnology in crop protection, emphasizing its importance in developing crops that are resistant to pests, diseases, and environmental stresses. By enhancing the natural defense mechanisms of plants, biotechnology reduces the need for chemical pesticides, thereby promoting more sustainable agricultural practices and minimizing the environmental impact of farming. The importance of biotechnology and genetic engineering in ensuring food security is a central theme of this chapter. As the global population continues to grow, the demand for food is expected to increase dramatically. Biotechnology offers a viable solution to meet this demand by enabling the production of higheryielding and more resilient crops. This section also explores the ethical and regulatory considerations surrounding the use of GMOs and other biotechnological innovations in agriculture.

    16.1 Introduction
Guava (Psidium guajava L.) is one of the most important tropical fruit crops, valued for its vitamin C content, nutraceutical compounds, and adaptability to diverse agro-climatic conditions. However, guava production faces problems of seedling variability, prolonged juvenile phase, susceptibility to wilt and anthracnose, and poor success rates of vegetative propagation (Singh et al., 2022).
Biotechnological tools, ranging from micropropagation to molecular diagnostics, provide solutions to these challenges. These methods complement conventional horticultural practices by ensuring clonal fidelity, enabling rapid multiplication of elite genotypes, characterizing genetic diversity, and offering prospects for disease resistance and quality improvement (Kumari et al., 2021; Mishra et al., 2020).
16.2 Micropropagation: Commercial-scale Clonal Propagation
Micropropagation is the most widely used biotechnology tool in guava. Nodal segments and shoot tips cultured on Murashige and Skoog (MS) medium with cytokinins such as benzylaminopurine (BAP) can produce multiple shoots, while rooting is induced using auxins like indole-3-butyric acid (IBA) (Singh et al., 2022).
Juvenile explants generally perform better, and the addition of antioxidants (ascorbic acid, polyvinylpyrrolidone) or activated charcoal reduces tissue browning. Plants require careful acclimatization under mist chambers before field planting. Micropropagation ensures large-scale, disease-free, uniform planting material for high-density orchards (Singh et al., 2022).

Plant Pathogen

Pathogen is an inducer of disease.

A pathogen can therefore be defined as any factor that induces unresolved impairment or disruption of one or more important plant functions.

Some plant functions such as nutrient uptake, are highly localized. Others, such as respiration, occur in all living tissues. Still others, such as photosynthesis and photorespiration, are extensively coordinated. Pathogen effects therefore range from very local to very general.

It is difficult to define ‘stress’ precisely. In engineering and the physical sciences, it is usually defined as the force applied per unit area. But as far as in biology, stress it is usually described more loosely as any factor that disturbs normal functioning of an organism. Any environmental factor potentially unfavorable to plant is termed as stress.

The effect of stress on plant condition is called strain. Stress is the action, whereas strain is the reaction. Stress may be defined as external conditions that adversely affect growth, development and productivity. Stress may further classified as:

• Zero stress: This is the level of exposure of plants to environmental factors that leads to neither injury nor reduction in growth and yield of crops.
• Stress escape: Capacity of the plant to complete its life cycle during wet season before onset of stress.

Abstract

Mungbean [Vigna radiata (L.) Wilczek] is an important pulse crop grown in India. It is a short duration annual with yellow flowers and fuzzy brown pods. Majority of the mungbean grown in India is under rainfed conditions and the productivity of mungbean is not upto the mark to meet the daily dietary requirement of a populous country like India. The biotic and abiotic stresses in mungbean, majorly MYMV, powdery mildew, drought, salinity, water logging etc leaves a severe yield loss in mungbean crop. Among them, MYMV and powdery mildew diseases show yield losses accounting upto 85% and 40%, respectively in severe conditions. The present review paper is based on the study of both biotic and abiotic stresses and the recent advances in breeding for resistance to stresses. A detail explanation of major diseases and insect pests affecting mungbean are discussed along with the information of genomic resources and research done in mungbean in recent years. Recently SSR’s have been using widely for all molecular level research work like genetic diversity, constructing linkage maps for identifying QTL’s, marker assisted breeding etc. in mungbean. The resistant varieties developed against these biotic and abiotic stresses are listed which are very important for attaining a prominent yield in mungbean.

While Chapter 2 focused on how abiotic (non-living) factors like weather directly influence crops, this chapter explores the impact of biotic (living) factors that interfere with crop growth, development, and yield. Critically, we will also examine how meteorological conditions play a significant role in the prevalence, spread, and severity of these interferences. Biotic interferences refer to the detrimental effects of living organisms on cultivated crops, leading to reduced yield, quality, or even complete crop failure. The three main categories are pests, diseases, and weeds (Fig. 3.1).

Pests are organisms, primarily insects and mites, but also nematodes and some vertebrates, that directly damage crops by feeding on them, laying eggs in them, or transmitting diseases.

Abstract
Biotic stress in plants refers to the adverse impact of living organisms, such as pathogens, pests, and nematodes, on plant growth, development, and productivity. These biotic agents can infect, infest, or parasitize plants, triggering a range of physiological and biochemical responses that compromise plant health. Biotic stress can manifest in various forms, including fungal diseases, bacterial blights, viral infections, insect infestations, and nematode attacks, leading to reduced crop yields, lower quality produce, and significant economic losses. Understanding biotic stress is crucial for developing effective management strategies to protect plants and ensure food security, as it poses a significant threat to agricultural productivity and sustainability. To enhance the productivity of grain legumes, a critical food source for millions, mitigating biotic stress is essential. By adopting effective strategies to manage biotic stressors like diseases, pests, and nematodes, legume yields can be significantly increased.
This can be achieved through the development and deployment of resistant cultivars, integrated pest management (IPM) practices, cultural methods like crop rotation and sanitation, and biological control methods. Additionally, rhizobia and mycorrhizal inoculation can enhance plant defense and nutrient uptake, while precision agriculture techniques facilitate early detection and targeted management of biotic stress. By implementing these approaches, legume productivity can be boosted, ensuring food security, improving livelihoods, and meeting the growing demand for this vital crop.
Keywords: Biotic stress, Integrated pest management, Legume productivity, Sustainability

Stress is an intrinsic part of biological systems, and successfully adapting to stimuli that induce stress is essential for the survival of dryland crops and farm animals kept in a complex and ever-changing environment. Here the biotic stresses of crop plants are treated and not that of farm animals to avoid the expansion of the volume of the chapter. Biotic stress in plants is caused by living organisms, specifically viruses, bacteria, fungi, nematodes, insects, arachnids, and weeds. In contrast to abiotic stress caused by environmental factors such as drought and heat. Biotic stress agents directly deprive their host of its nutrients leading to reduced plant vigour and, in extreme cases, death of the host plant. In agriculture, biotic stress is a major cause of pre- and postharvest losses. Plants respond to biotic stress through a defence system (Atkinson and Urwin, 2012).

2.1 Insect Pests

Insects are often a major obstacle to the successful production of cucurbits (York, 1992). They can severely reduce yield, injure or kill plants, spread diseases and adversely affect fruit quality. A number of insect pests, mites and nematodes have been found to infest cucurbitaceous vegetables but fortunately, in India, fruit flies and a few species of beetles are of economic importance; aphids and blister beetles though of regular occurrence, seldom cause severe damage.
 
Other insect pests reported to infest the cucurbitaceous vegetables are pumpkin caterpillar, Diaphania indica (Sounders), snake gourd semilooper, Plusia peponis Fab., white fly, Bemisia tabaci Genn., stink bugs, Apspongopus spp. and flea beetle, Phyllotreta spp. etc. These biotic pressures on the crops are increasing day by day due to round-the-year intensive cultivation of photo and thermo insensitive cultivars as well as indiscriminate use of toxic agro-chemicals in the present era of chemicalized agriculture.

Phytonematodes spend much of their lives inside or in contact with host tissue, and molecular interactions constantly occur and shape the outcome of parasitism.Eggs of these parasites generally hatch in the soil, and the juveniles must locate and infect an appropriate host before their stored energy is exhausted Components of host exudate are evaluated by the nematode and direct its migration to its infection site.Host plants recognize approaching nematodes before physical contact through molecules released by the nematodes and launch a defense response In turn, nematodes deploy numerous mechanisms to counteract plant defenses. A review by Shahid Siddique et al., (2022) focused on these early stages of the interaction between plants and nematodes and discussed how nematodes perceive and find suitable hosts, how plants perceive and mount a defense response against the approaching parasites, and how nematodes fight back against host defenses.

3.1 Biowarfare

Biowarfare, also known as Biological warfare or germ warfare, is the use of biological toxins or infectious agents such as bacteria, viruses, insects, and fungi with the intent to kill, harm or incapacitate humans, animals or plants as an act of war. Biological weapons (often termed “bio-weapons”, “biological threat agents”, or “bio-agents”) are living organisms or replicating entities (like viruses, which are not universally considered “alive”). Entomological (insect) warfare is a subtype of biological warfare.

Offensive biological warfare is prohibited under customary international humanitarian law and several international treaties. 

Bird of Paradise, belonging to the genus Strelitzia, is a distinctive ornamental cut flower recognized for its striking inflorescences and architectural form. This chapter covers its origin, botanical features, species, varieties and commercial importance. It explains environmental requirements, soil conditions, propagation, planting, irrigation, nutrient management and cultural operations. The chapter also addresses pests, diseases, harvesting, grading and postharvest handling. Because of its unique appearance and suitability for floral arrangements, Bird of Paradise has significant potential in premium floriculture markets. The chapter provides practical production guidance for growers seeking to develop high-value tropical cut flower enterprises. commercially and sustainably.