eChapters

The glucose is utilized in the body for immediate energy requirements. The utilization of glucose depends on the integrated activity of digestive system metabolism and endocrine system of the body. Glucose tolerance is referred as the capacity of animal to regulate elevated blood glucose on ingestion or infusion, gradually. The level of glucose that can be tolerated by an animal without producing glucosurea is indicative of tolerance. Glucose load challenges the pancreas to release insulin which in turn regulates blood glucose level. If the insulin release is adequate and target cells have healthy receptors, elevated blood glucose level return to base value within 2 hours and glucose does not appear in urine. An increased glucose tolerance means limited rise and rapid fall in blood glucose in short span than the normal rate of utilization.

Blood Cholesterol

(Colorimetric Method of Lieberman, 1885: Burchard 1889 Modified by Watson, 1960).

Introduction

According to Liebermann-Burchard reaction, treatment of chloesterol with acetic anhydride and concentrated sulphuric acid in a water-free environment, polymerized unsaturated hydrocarbon with an intense blue-green colour are produced. The values for cholesterol esters may be calculated by deducting free cholesterol from total cholesterol.

We must know our family, its members and their relationships. Let us first look at basic relationships which often figure in the questions. If you are conversant with the given items in the table below, half of your battle is already won. It is not required to mug-up these names, but instead make a clear picture in your mind as you go through each one of them. This will go a long way in retaining the details of these given shortcut tricks for solving Blood relations problems for logical and analytical reasoning. Read the following thoroughly before moving on

Blood transfusion is an important supportive therapy for animals with severe anaemia, blood loss and other conditions requiring restoration of circulating blood components. This chapter presents practical principles of blood transfusion, including blood groups, donor selection, blood collection, compatibility testing and administration. Cross-matching is important for reducing the risk of transfusion reactions. The chapter also discusses monitoring of patients during transfusion and recognition of common clinical signs associated with transfusion reactions. Proper collection, storage, handling and administration of blood products are essential for safe and effective therapy. The practical procedures help veterinary students understand transfusion medicine and patient management.

Blood transfusion is one of the crucial life-saving procedures in veterinary medicine undertaken to increase red cell mass, volume replacement, replenishing coagulation factors, and protein replacement. The concept of blood transfusion was first introduced by Francis Potter in 1639. However, it was only in 1666 that Richard Lower reported the first successful direct transfusion from one dog to another. Blood is a biological drug and is not a definitive treatment, but it provides support until the disease is diagnosed or treatment is initiated. Indications Blood transfusion is indicated for the management of the following conditions: •     Anemia due to various diseases/disorders, like infection or acute/ chronic hemorrhage •     Bleeding disorders such as thrombocytopenia or coagulopathies •     Rodenticide toxicities

22.1 Introduction

The blood vascular system of fishes is modified to suit the aquatic mode of life. Fish contains the simplest single-circuit closed blood vascular system.

The structure of the heart in fish varies little. In fish, all of the chambers of the heart are essentially the same. The size and shape of the component chambers may differ. A large heart is found in extremely sluggish fishes with low oxygen requirements. The number and arrangement of valves in the conus arteriosus are the most distinguishing features in various fishes.

Blood is a first by-product of animal slaughter house. It accounts about 5-7 percent of animals live weight. It is highly nutritious material particularly rich in protein (17-18%) and essential nutrients. Blood is a rich source of amino acids like leucine, lysine, valine, phenylalanine, histidine etc. It is also rich in haem iron which is biologically most available form of iron. Protein efficiency ratio of plasma is 2.5 while 1.0 for globin. It contains about 80 percent water and responsible for effluent problems. It is a great source of pollution because BOD₅ of whole blood is 250000 mg/litre. It should be collected and processed as soon as possible because whole blood clots easily and is capable to clogs the drainage system, grease traps and septic tank of meat plant or abattoir.

Whenever we get a cut, a red fluid comes out of the wound. This red fluid is called blood. Blood is very important for the body as is evident from the fact that the loss of blood from the body makes it weak and may even cause death. Physiologically the blood is a red tissue composed of cells suspended in a pale yellow fluid, plasma. Unicellular organisms that live in immediate contact with the external environment obtain nutrients directly from that environment and eliminate unused or unwanted materials. Blood and lymph are important connecting fluids among the diverse anatomical structures of the mammalian organisms. Thus blood is the liquid vehicle by which the major organic nutrients are transported from the intestine where they are absorbed, to the liver where they are processed and thence to other organs. Similarly blood is the liquid vehicle for the transport of waste materials from various organs to the kidney and gastrointestinal tract from where they are eliminated. Thus the blood is the major transport carrier of nutrients, metabolites, oxygen, hormones, waste products etc. inside the body. Blood is an important medium to maintain homeostasis of various electrolytes in the body. Besides blood provides the army against any external attack on the body by harmful microorganisms. This army is in the form of cells, called white blood or corpuscles or leucocytes.

In a normal adult human being, about 5 to 6 litre of blood is present which is about 8 percent of the body weight. In domestic animals generally 5 to 8 per cent of the body weight is blood (say 30-40 litre in a cow). The blood is composed of two parts, the cells and the fluid part or plasma. The cellular constituents of blood include erythrocytes or red blood corpuscles, leucocytes or white blood corpuscles and thrombocytes or platelets. The cellular constituents of blood varies with the species as well as with the age and physiological stage of the animal.

The extracellular constituents or plasma is composed of water, electrolytes, proteins, lipids, glucose, enzymes and hormones etc. The blood undergoes coagulation on exposure to air leaving clear watery fluid, called serum. The serum is nothing but plasma minus fibrinogen and other clotting factors. The serum is a clear yellowish fluid. The yellow colour is due to the presence of small quantities of bilirubin, a bile pigment and of carotenoids. The blood serum of cattle is yellow due to presence of more of carotenoids whereas that of buffalo is white due to the absence of carotenoids. The blood clot consists of a network of fibrous strands of fibrin in which are enmeshed the cellular elements of the blood. Blood clotting is a protective mechanism against excessive loss of blood from the body. The lymph also clots, although, somewhat more slowly than blood.

Blood is the fluid pumped by the heart that circulates throughout the body via the arteries, veins, and capillaries . Blood is a body fluid in animals and human beings that delivers necessary substances such as nutrients and oxygen to the cells and transports metabolic waste products away from those same cells. Hematology is the science or study of blood, blood-forming organs and blood diseases.

Blotting is the procedure through which nucleic acids or proteins are immobilized onto a solid support generally nylon or nitrocellulose membranes. Blotting of nucleic acid is the central technique for labeling and hybridization on membranes have formed the basis for a range of experimental techniques involving understanding of gene expression, organization, etc. More recently the identification of abnormal genes in genomic DNA has become increasingly important in clinical research and genetic counseling. We can identify inherited disease, infectious agent present in the sample by using blotting technique. It is also used to mapping restriction sites in single copy gene.

Southern Blotting
Introduction

Southern hybridization was named after Edward M. Southern who developed this procedure at Edinburgh University in the 1970s. To oversimplify, DNA molecules are transferred from an agarose gel onto a membrane. Southern blotting is designed to locate a particular sequence of DNA within a complex mixture. It is also called Southern blotting since the procedure for transfer of DNA from the gel to the nitrocellulose filter resembles blotting. In Southern hybridization, a sample of DNA containing fragments of different sizes is subjected to electrophoresis using either polyacrylamide or agarose gel.

1. Introduction

With the advancement of science and technology, new technologies have been developed to advance molecular work to a great extent. Thousands of DNA molecules can be simultaneously sequenced at one time using next-generation sequencing (NGS). Next-generation sequencing (NGS) technologies act as revolutionary tools for various implementations and can produce hundreds of gigabases of sequence data within a single experiment. A wide variety of modern sequencing technologies go by the name of "high-throughput sequencing." In recent years, this technology has advanced as a de facto tool for the study of genomics and epigenomics. Nucleotides can be located using this technique anywhere in the genome or in groups of specific DNA or RNA regions. An NGS plot is a useful tool for bridging the gap between enormous datasets and genomic information in this era of massive sequencing data. Resequencing of genomic DNA will undoubtedly be the primary application of NGS in clinical settings. Whole genome sequencing (WGS) offers an intricate representation of single nucleotide variations (SNV), insertions and deletions (indels), complex structural alterations, and changes in copy number, all of which are attainable through a single analysis. Extensive genetic information can be obtained and used after complete genome sequencing, which reveals a large amount of genetic data. However, a significant portion contains either novel information or data whose clinical significance has not yet been established. WGS is the most comprehensive genetic analysis of an individual or cancer genome.

BGA belong to a class of prokaryotic photosynthetic microorganisms also known, as cyanobacteria are capable of fixing atmospheric nitrogen aerobically. Most abundantly found in tropical zone, never form fibrous growth and are enveloped by a sheath. The trophic independence of algae for C and N makes them responsible for a natural fertility buildup of rice field soils. The nitrogen fixing ability of BGA was first recognized by Frank and Prantil in 1889. The occurrence of the BGA in the rice field was reported by FE Fritsch in 1907.

Rice is grown in about 40 million hectares of land in India and nitrogen requirement for the crop is 60 lakh tonnes based on the present levels of consumption. Rice production has to be sustained and increased to provide staple diet to our increasing population. Blue green algae is a potentional biofertilizer in rice because of their capacity to meet both carbon and nitrogen requirement naturally from the air. They are microscopic algae which are collectively called phytoplankton produce basic organic matter forming the first link in food chain and alongwith replenish the oxygen content of atmosphere. Algae contain proteins, Vitamins, iodine, bromine and substances of antibiotic and stimulatory nature. The soil algae are divided into chlorophyta (green algae) cynophyta (blue green) bacillariophyta (diatoms) and xanthophyta (yellow-greens).

De (1939) suggested that fertility of rice fields is maintained due to the growth of blue-green algae. Different types of blue-green algae have been reported to colonize the rice fields. The cells such as heterocysts in Anabaena, Nostoc, Tolypothrix etc. which have capacity to fix atmospheric nitrogen under aerobic condition and unicellular and non- hetercoystous forms also fix atmospheric nitrogen under microaerophilic conditions.

Synonyms : Catarrhal fever, Sore mouth, Epizootic catarrh, Sore muzzle, Pseudo foot and mouth disease, Malarial catarrhal fever.

It is an infectious, noncontagious arthropod borne viral disease of domestic and wild ruminants namely sheep, goat , cattle, camels, llamas, deer and antelopes. This is predominantly a disease of sheep but occasionally cattle and  goats are subclinically affected. The disease is characterized by high fever, catarrhal stomatitis, rhinitis, enteritis and lameness due to inflammation of the coronary bands and sensitive laminae of the feet. There are at least  25 serotypes of bluetongue virus worldwide within the BTV serogroup. Genetic variability occurs within a group due to genetic drift of individual gene segments and genetic reassortment when ruminants or other vectors are infected with more than one strain.

Abstract

Blumea lacera (Burm f.) DC. belonging to family Asteraceae is an annual herb with a strong odour. This herb is distributed throughout the plains of North-west India upto an elevation of 2,900 m and is also reported from Tropical Africa, South East Asia, Bangladesh, Nepal, Indonesia, Malaysia, Philippines, Thailand and Vietnam. Blumea lacera is a plant with camphor-like odour, and is highly variable, grows in grassland, field, roadside and forest edge. Traditionally, it is used internally and externally as a hemostatic and anti-inflammatory agent. The juice of leaves along with black pepper (Piper nigrum) is given to cure bleeding piles. This herb is also offered as anthelmintic (especially for threadworm). Leaves and roots are used as astringent, diuretic and febrifuge and even in cuts and wounds. Bruises and ulcers can also be treated by applying fresh juice or extract. For treating piles (Hemorrhoids), the leaves of B. lacera has been used in ethnomedicinal practices.The phytoconstituents present in leaves are mainly terpenoids, glycosides, volatile oils, some glycoalkaloids and many more yet to be determined. B. lacera has been used as an ingredient in many pharmaceutical preparations. The chapter gives a brief idea about the ethnomedicinal usage, phytochemical profile, pharmacological usage and the future perspective of B. lacera. and would help the researchers for better understanding of this plant as well as developing plant derived molecules in drug discovery process.

In most of the examinations, the questions related to boat and stream are asked. These questions are seemed to be very difficult but are very ticklish and easy to solve. The students need to concentrate on flow of stream upward and down ward. If the boat is moving towards the flow (downstream), the speed of boat and stream will be added and if the boat is moving towards opposite of stream flow (upstream), the distance covered by the baot will be equal to boat speed minus strean speed. Few important tips are given below to solve such type of questions.

Downstream and Upstream Motion

Downstream motion of a boat is its motion in the same direction as the flow of the river. Upstream motion is exactly the opposite. There are two parameters in these problems.

1. INTRODUCTION

Water is the source that upholds every part of life on earth and is a key element of sustainable development. It is essential to the survival of healthy and safe lives of mankind. Ecosystems are inevitably linked with water. Lack of access to ample and safe drinking water is a serious setback among human population. Noxious substances from industries when enter into lakes, streams, rivers, oceans, and other water bodies, they get dissolved or lie suspended in water or get deposited on the bed. This results in the pollution of water whereby the quality of the water deteriorates, affecting aquatic ecosystems.

The strain of escalating population, growth of industries, urbanization, energy intensive life style, loss of forest cover, lack of environmental awareness and implementation of environmental rules and regulations, environment improvement plans, untreated effluent discharge from industries and municipalities, use of non-biodegradable pesticides/fungicides/herbicides/ insecticides, use of chemical fertilizers instead of organic manures, etc are basis of water pollution. The pollutants from industrial discharge and sewage, besides finding their way to surface water reservoirs and rivers are also percolating into ground to pollute ground water sources, threatening food security, access to safe drinking and bathing water and providing a major health and environmental management challenge. Contaminated water from inadequate waste water management provides one of the greatest health challenges restricting development and increasing poverty through costs to health care.

Water pollution is a sensitive problem all over the world. In the wake of increasing urbanization and industrialization, the pollution potential is gaining thrust day by day. Monitoring of waste water quality parameters is currently a subject of growing concern globally. Oxygen, although poorly soluble in water, is fundamental to aquatic life. In the absence of free dissolved oxygen, aquatic systems become inhabitable to most aquatic flora and fauna. Because the solubility of oxygen in water is so low, even little decrease in dissolved oxygen levels can compromise the health of natural water systems. Organic pollutants requiring oxygen for their decomposition exert a rapid and direct influence on the ecology of these environments. Therefore it is required to monitor the load of organic pollutants in the waste water.

This chapter examines the human body as an integrated working system governed by anatomical, physiological and biomechanical principles. It describes the roles of the musculoskeletal, nervous, cardiovascular, respiratory and metabolic systems in movement, strength, coordination and endurance. Muscle strength, joint range of motion, posture, flexibility and physical work capacity are discussed in relation to workplace demands. The chapter distinguishes static and dynamic work and explains how unsuitable loads or repetitive tasks contribute to fatigue and injury. Individual differences associated with age, fitness, body dimensions and physical capability are also considered. These concepts provide a scientific foundation for setting safe workloads and designing tasks around natural human limitations.

Body condition scoring (BCS), although subjective in nature, is the only practical method of evaluation of body energy stores in dairy cows. The method of body condition scoring, originally proposed by Edmonson et al. (1987) and recommended by the NRC (2001) is described here. This method uses a 5 point scale, with BCS of 1 being extremely thin and a score of 5 being extremely fat. This system includes a combination of both visual appraisal and manual palpation on 8 separate body locations. Figure 1 shows the suggested BCS chart based on these locations. The assessor can score the cows using this chart with a fair accuracy, without much previous experience of scoring the cows. The scoring method is recommended for HF and Holstein crossbred cows.

Loss of BCS is expected during early lactation when a cow is mobilizing body fat in support of energy needs for lactation. Typical observed changes in BCS range from 0.5 to 1.0 condition score units during the first 60 days postpartum. A 1-unit decrease in BCS for a cow weighing 650 kg at calving (BCS 4) would provide 698 MCal of ME.That amount of ME is sufficient to support 564 kg of 4 per cent fat corrected milk.