eChapters

Introduction

Food ingredients sector is indicating ample prospects for growth. The segment is an obligatory element for the growth of the food and beverage sector. These ingredients are also driving the development of the healthy functional foods industry to increase by 20% growth annually. Innovation in the food and beverage market, especially in the processed food domain, has created innumerable opportunities for new product development in the ingredients market as well. The segment is driven by the growing food processing industry and India is emerging as ingredients bowl of the future. Additionally, growing urbanization coupled with industrialization and changing lifestyles have led to growing demand for convenient food that offers health benefits, over and above the basic nutritional benefits of the foodstuff. There are nearly 10 to 15 broad classes of ingredients that include the very basic ingredients as well as certain value added ingredients that have niche applications. Key ingredients are preservatives, flavours, colours, hydrocolloids/stabilizers, emulsifiers, artificial sweeteners and value added health food ingredients such as omega-3-fatty acids prebiotics, probiotics etc. Until recently, there has been limited research into functional foods in meats, while functional foods in the dairy sector have been developed to a high degree. But meat is less allergenic than many other foods and much more research into the development and marketing of novel functional meat products is required. Such meat products would open up a new market for the meat industry. Nutraceutical and functional food industry is a very vibrant industry today with the claims and a clear mandate of health, wellness and prevention of diseases as one of its main goals.

Introduction

India is considered as the “Home of Spices”. They are a prized group of minor components and have been an integral ingredient of Indian diet. Since antiquity, spices form an important part of the Ayurvedic Pharmacopoeia (The Indian System of Medicine), with their widespread traditional use. With its wide climatic conditions and topographical features, India naturally possesses wide variety of medicinal flora. The country enjoys the distinction of being the largest producer and consumer of spices as well as the fastest growing spice market in the world.

Spices are potent plant foods that possess a wide range of nutrient bioactive compounds that contribute to improve health, while adding variety, colour, flavour and aroma to daily diet. The term spice or condiment applies to “such natural plant or vegetable products or mixtures thereof, in whole or ground form, as are used for imparting flavour, aroma and piquancy to and for seasoning food” (Sikri and Berwal, 2008).

Webster (2011) describes spices as ‘Any of various aromatic vegetable productions as pepper, cinnamon, nutmeg, mace, allspice, ginger, cloves, etc., used in cookery to season and to flavour sauces, pickles, etc.; a vegetable condiment or relish, usually in the form of a powder; also, as condiments collectively’.

Introduction

Biological activation of grain or germination is a natural, biological processing techniques and traditional method that can be used to improve the nutritional, functional, and sensory properties of grains. During biological activation of grains, extensive phytochemical changes were reported in a variety of food grains, which reflects a dynamic and complex nutrient flux including remobilization, degradation and accumulation. During this process many hydrolytic enzymes were activated to hydrolyze the starches, proteins and, non-starch polysaccharides, which results in more oligosaccharides and free amino acids. Many nutrients and bioactive compounds reportedly increase and then decrease as they are utilized by the growing plant, which results in degradation of the high molecular weight polymers for generation of bio-functional substances. The biological activation of grains also realizes a significant growth of nutritional value by increasing the bioavailability of nutritional compounds, vitamins, bio-elements and other biologically active substances due to the partial hydrolysis of starch, proteins, hemicelluloses and even celluloses. Biological activation triggers the enzymatic activity in grain and dormant hydrolytic enzymes are activated, breaking down starch, fibers and proteins and leading to an increase in the amount of digestible compounds along with improvement of functional properties without any chemical modification. Also, the activity of some anti-nutritional factors (enzyme inhibitors, haemagglutinins, anti-vitamins etc.) decreases or disappears during activation, allowing a complete valorization of biological compounds in grains. During biological activation hydrolytic amylase enzymes, proteolytic enzymes, and catalase were activated in grains, which enhances the starch degradation, a complex biochemical process and thus lead to the conversion of insoluble granules to soluble starch and dextrin. Apart from positive shifting in the level of nutrition, biochemical activities which occur during activation also generate bioactive components of health-promoting activities through the enzymatic actions that are antioxidants in nature, such as ascorbic acid, tocopherols, tocotrienols and phenolic compounds, thus resulting in an increase of antioxidant activity.

Introduction

Pulses are annually grown leguminous crops that yield one to twelve grains/ seeds within a pod, and are often promoted in diet owing to their low cost and many beneficial nutritional effects (Craig 2009). The United Nations general assembly has declared 2016 as the international year of pulses (Oyeyinka et al. 2016). The Food and Agriculture Organization (FAO) describes pulses as the crops that are harvested only for dry grain. This definition excludes crops that are harvested green as food sources (such as green beans and green peas), crops that are used only for sowing purposes (such as alfalfa and clover) and crops used primarily for oil extraction (such as peanuts and soybean). India is the largest producer (around 25%) of pulses in the world and the Indian subcontinent covers the leading area in terms of consumption of pulses. It was also suggested that India can also increase the production output by 30%, considering a rise in government support prices for stimulating the growth of pulses and in case of a normal monsoon season (FAOSTAT 2008). The mainly grown pulses in India include mungbean (green gram), chickpea (Bengal gram), urd bean (black gram), lentil (masur), pigeon pea (arhar), cowpea, horse gram and peas (Tiwari and Singh 2012). In the western countries, nowadays pulses are one of the preferred food sources of the population, owing to ideological reasons and interest in vegetarian diet.

Introduction

Functional elements are non-conventional biomolecules in foods that have the ability to modulate one or more body metabolic processes or pathways, leading in health advantages and well-being promotion. Research has demonstrated a connection between food, health and well-being functional elements. Consequently, functional elements have health-promoting roles connected with multiple progressive steps at various phases of disease control, from initiation to growth. They can therefore be implemented efficiently in disease therapy and prevention. Therefore, the borderline between food and medicine is becoming very thin at a moment when the role of a healthy diet in stopping non-communicable diseases is well recognized. Functional elements include plant-derived, non-nutritious and biologically active chemicals that operate in the body to avoid certain non-communicable diseases from occurring.

Food contains over 900 phytochemicals. One fruit or vegetable serving (about 120g) can contain as many as 100 distinct phytochemicals. Previously, functional elements were believed to happen predominantly only as phytochemicals in plant products including whole grains, fruits and vegetables. However, in animal goods such as milk, fermented dairy products and cold water fish, probiotics, conjugated linolenic acid, long-chain omega-3, −6 and −9 polyunsaturated fatty acids and bioactive peptides are also discovered. Usually, functional elements happen in various forms in food such as glycosylated, esterified, thiolated or hydroxylated materials. They also have numerous metabolic operations that enable positive impacts in the body on several illnesses and target tissues (Balic et al. 2020).

Introduction

Fruits contain various beneficial compounds that possess biological activities and can be used as pharmaceutical nutraceutical products. Most of the fruits contain polyphenolic components that can promote antioxidant potential on human health. Wild Blueberries, strawberries, plums, oranges, grapes, cherries and mangoes are rich in antioxidant. Additionally, anti-inflammatory, antibacterial, antifungal and chemopreventive effects are associated with the various fruits. Besides polyphenols, other compounds such as xanthones, carotenoids, saponins also exhibit health effects. Fruit consumption is considerably increasing in the daily diet because they supply high levels of biologically active compounds that impart health benefits beyond basic nutrition. Fruits provide an optimal mixture of antioxidants such as vitamins C and E, polyphenols and carotenoids, vitamins, phytoestrogens, glucosinolates and anthocyanins. Diets rich in fruits may help to delay the ageing processes and reduce the risk of certain types of cancer, cardiovascular diseases and other chronic diseases. The combination of vitamins, minerals, phenolic, antioxidants and fibre seems to be responsible for these effects. The most dietary components in fruits acting as antioxidants are fibre, polyphenols, conjugated isomers of linoleic acid, D-limolene, epigallocatechin, gallate, soy protein, isoflavones, vitamins A, B, C and E (group of tocopherols), calcium, selenium, chlorophyllin, alipharin, sulphides, catechin, tetrahy-drocurecumin, seasaminol, glutathione, uric acid, indoles, thiocyanates and protease inhibitors.

Introduction

Consumers are nowadays very much concerned about the food they purchase and consume. Consumer wants the food containing antioxidant properties or can say functional food in safe and stable forms, so that the availability of daily requirements of functional food ingredient must be ensured for normal growth and development of the body. The functional food that can provide a health benefit such as increased dietary fibre, vitamins or minerals to the body. The incorporation of antioxidant compounds acts as an efficient way to fight against reactive oxygen species (ROS) generated in our body. As it is already known that the ROS causes oxidative stress and acts as a main trigger for different life threatening chronic diseases like cancer, diabetes, cardiovascular disease, aging etc. Therefore, seeing the consumers’ demand for value added products with bioactive ingredients, all small and large scale food industries are taking initiatives to process different types of food (fruits, vegetables, dairy, sea food, animal, microbial products etc.) to extract and purify its active fraction of functional ingredients to valorize different value added products. Functional ingredients may also be derived from different food wastes generated during processing of food. This practice provides additional economic benefits to food businesses. Food industries are also giving importance to different processing methods to extract high quality of functional food ingredients in large quantity because processing method affects the concentration of extracted bioactive compounds. The simple addition of functional ingredients may cause an unwanted and negative change of sensory and texture of food. Therefore, using novel processing technologies such as microencapsulation, the negative changes could be minimized or avoided because these technologies could prevent the inactivation of bioactive ingredients.

Introduction

Our traditional knowledge of medicine has been one of the strongest areas of research because of our rich heritage in herbal plants & great biodiversity in our fauna & flora. Consumption of plant foods and the structurally diverse natural products provided by them have long been associated with health-protective and therapeutic effects. Natural products in plant foods have been shown to have pleiotropic therapeutic and chemopreventive properties. These bioactive products are known to potentiate the efficacy of other drugs and have synergistic effects too. Most of the prescription drugs discovered so far is from plant sources and 75% drugs had been claimed in folklore claims and traditional medicinal system (Reynold, 1991; Benowitz, 1996). The traditional Indian medicinal system utilizes multicomponent formulations of plant extracts which may either act synergistically or avoid many side effects and might have added up trace element through combination of different plant extracts which could have additive effects. However the effort to utilize traditional knowledge in the light of modern scientific findings is lacking in India. Accumulating evidences as well as in-vitro studies implicates inverse relationship between regular consumption of fruits vegetable and the risk of specific cancer. Diet derived nutritional compound mediated interventions in the process of tumorigenesis may be promising chemopreventive strategy with minimal toxicities to the physiological system.

Introduction

Society is dynamic. The requirement of society varies in course of time. During the life process human being faces lots of health hazards which are mostly due to intake of various foods. So people become cautious about the intake of food and think about the quality of foods which can protect the health of human being. In ancient times people made medicines from plant extracts to protect their health. In 1989, Dr. Stephen coined the term “Nutraceuticals” which is combination of nutrition and pharmaceutical. Nutrition is required for health and pharmaceuticals provide remedy for sickness. So it is a preventive approach. Nowadays, the definition of nutraceuticals has been expanded to include vitamins, minerals, herbs and other botanicals, amino acids, and dietary substance for human use as a supplement diet (Stauffer, 1999).

Nutraceuticals are dietary supplements made only from whole foods to augment health. Currently over 470 nutraceutical and functional food products are available with documented health benefits (Brower, 1999). The patients are much aware about the side effects of chemical agents in short and long term therapy. So to avoid medicine used for small diseases, new therapies came into trend with the help of nutritional approaches i.e. “Nutraceuticals”. The use of nutraceuticals becomes easier than pharmaceuticals since nutraceuticals does not require any animal verification for its use in human. Nutraceuticals,

Functional foods and Dietary Supplements are most important concept in food science. They have distinction amongst themselves.

Introduction

The health and wellness continue to drive growth in the global food industry as the consumers are constantly looking for the foods, beverages and other dietary solutions that will help them more aggressively to meet their own unique nutrition and personal health goals. Worldwide countries are going through a nutritional transition and are now affected by double burden of problems like nutritional and food security. The common alarming problems are under nutrition and specific nutrient deficiencies simultaneously coupled with imbalanced diets and chronic diseases. The problem of chronic diseases, non-communicable diseases (cardiovascular diseases, respiratory diseases, diabetes, obesity, cancer etc.) and malnutrition is more serious in the developing countries. The lack of access to health services, sufficient income, healthy and nutritious foods characterize majority of poor people in developing countries. So, food is not only the solution but also viewed as a product to enhance health and wellbeing, and it encourages the producers by supplying new goods that meet these needs. From the past decade, there has been growing consumer awareness regarding health, nutrition and the food safety which are often triggered by specific food incidents, especially in the developed countries and high-income groups in such countries. In the food industry there have been two broad trends which are hazards in the food chain and nutritional profile of the food. There has been recognition that hazards and contaminants can enter the food chain and can cause sickness, death and diseases, and so governments, often to deal with consumer and industry concerns, establish regulations and standards on food hazards including standards on pesticides, antibiotics, microbiological hazards and other chemical and physical contaminants. On the other hand, there is also a growing awareness of the health-enhancing properties for prevention and treatment of health concerns beyond the basic nutritional component of many foods.

Indian Institute of Horticultural Research (IIHR), Bangalore

Indian Institute of Vegetable Research (IIVR), Varanasi

Indian Institute of Spices Research (IISR), Calicut, Kerala

Central Institute of sub-tropical Horticulture (CISH), Lucknow

Central Institute of Temperate Horticulture (CITH), Srinagar

Central Potato Research Institute (CPRI), Kufri, Shimla

Central Tuber Crops Research Institute (CTCRI), Thiruvananthapuram, Kerala

Central Plantation Crops Research Institute (CPCRI) Kasargod, Kerala

Central Institute of Arid Horticulture (CIAH), Bikaner, Rajasthan

Central Institute of Post Harvest Engineering and Technology (CIPHET), Ferozepur, Punjab

Importance of training and pruning

1. To control the habit of vegetative growth and regular bearing.
2. Remove all limbs and dead branches.
3. To improve flowering, fruiting and fruit quality
4. Remove all broken shoots, diseased, or pest infection branches from all trees and shrubs.
5. It is usually best to prune temperate fruits and shrubs during early spring before full leaf.
6. Maintain balance between shoots and roots at planting and nutrients uptake
7. Provide the basic tree form and to aid the development of a strong tree framework by encouraging strong wide crotch angles
8. Stimulate shoot growth near a cut.
9. Permit proper light distribution and penetration to the tree canopy.
10. Increase insect and disease control by allowing for better spray penetration.
11. Facilitate harvesting.
12. Achieve orchard uniformity.
13. Produce the proper amount of well distributed fruiting wood.
14. Encourage a structurally sound tree by removing branches with weak narrow crotch angles or those with diseased, damaged, or unproductive wood. Invigorate and renew the bearing area of older trees.

Selection of orchard soil in rainfed areas

A good or adequate soil for orchard production is to say that a given plot or area of land must possess the following qualities.

1. A deep and well aerated soil with good drainage.
2. Adequate nutrients for optimum tree growth and high yields.
3. Good Water holding capacity - the ability to store and feed the fruit tree with an abundant supply of water.

It is a scientific technique which provides maximum outputs of various inputs consistently without any loss of fertilizers and manure, plant, plant protection chemicals, produce etc. Therefore, one should understand the management of these qualities of both resource and output.

Plant Propagation

Plant propagation involves the formation and development of new individuals, which are used in establishment of new plantings. It is simply the reproduction or multiplication of a plant from a source that is often referred to as a mother plant. In general, two methods are employed: 1) sexual and 2) asexual. Sexual propagation is multiplication of plants from seed and asexual or vegetative propagation involves starting a new plant from some vegetative part of a plant. Propagation by seed results in seedling variability. It is an extreme disadvantage for a nursery operator trying to produce a uniform crop containing chosen desirable characteristics. Seed propagation is not practiced in fruit nursery as plants raised by this method fail to bear true to type fruits.

Germination of seed

The activation of the metabolic machinery of the embryo leading to the emergence of a new seeding plant is known as germination. Germination is essentially a quickening of the growth of the embryo. As germination proceeds, the growing points of the radical and plumule divide rather rapidly Usually the radical emerges from the seed coat first, proceeds downward, and develops into the root system, the plumule proceeds upward and develops into the shoot system. Germination is entirely a food utilization process. Processes going on in seed during germination are: 1) Absorption of water; 2) Secretion of enzymes and hormones; 3) Hydrolysis of stored food into soluble form; and 4) Translocation of soluble foods and hormones to the growing points. These processes are either wholly or in part influenced by the food reserves, hormone supply, water supply, oxygen supply and temperature level.

The plan reviewing the arrangement of fruit plants in an orchard is known as the orchard layout. Although several systems of planting are followed to increase the production of orchard, selection of root stocks, soil, system of training pruning and cultivars are very important factor influencing designing of layout. However, improper system results in overlapping of plant parts, competition for water, light, nutrient and unequal distribution of water etc., which ultimately results in poor performance or fruit orchard.

Layout plan

The marking of position of the plant in the field is referred as layout. The layout plan of the orchard should be prepared carefully, preferably in consultation with horticultural experts or fruit scientists. The orchard layout plan includes the system of planning provision for orchard paths, roads, water channels and farm building. A sketch of the proposed orchard should be prepared before the actual planting is taken up.

Minor fruit crops are considered important for their food, fibre, fodder, oil or medicinal properties, but have been reduced in value over time owing to particular supply and use constraints. These can include poor shelf life, unrecognized nutritional value, poor consumer awareness and reputational problems food or “poor people’s food”. The main products consumed by people are fruit cordial, jam, fruit drinks, chutney, candy, pickle and squash concentrate crop species. The entire country is mainly depend on handful of crop species neglecting large number of vegetables, fruits and other crop species with high nutritional and medicinal values which were grown traditionally in the past, resulting these valuable crop species are critically facing the state of speedy disappearing.

Soil

Soil is the upper most crust of earth surface which supports plant growth. It is defined as a three phase system in which plants grow. These phases are solid, liquid, and gas are essential. Solid part is frame which provides space for other two. It consists of minerals, clay minerals and organic matter. The soil is also a living system with millions of microbes that breakdown organic matter. The microbes are very essential and survive only when soil is well aerated and rich in organic matter and devoid of waterlogged conditions. Texture of soil depends on the size of solid particles and classified as gravel, coarse and fine sand, silt and clay. Soils are classified according to the relative distribution of these particles. Likewise, arrangement of these particles is referred as structure. Generally loamy soils and crumb structure are most growing preferred for fruit crops. According to level of organic matter, soils are classified as mineral soil or organic soil and soil having more than 20% organic matter is organic soil like peat and muck etc. Minerals and salts lend chemical properties to the soil like pH, alkalinity, sodicity, salinity and cation exchange capacity which influences the availability of nutrients in soil. Therefore, for making choice for soil, soil analysis in terms of following criteria isessential to decide on land capability. Maximum numbers of fruit orchards in India are depleted from micro and macro plant nutrients which interrupted the growth and development of the fruit plants and their quality. Therefore, like growers are not attain profit desired. To obtain marketable fruits, nutrients managements are highly beneficial to the fruit growers rainfed areas. Analysis of soil is useful to determine the level of the elements available in the orchards to get maximum yield.

Major field operations for horticultural crops viz., fruit nursery/seedling preparation, post hole digging for planting, intercultural operation, aeration, training and pruning, irrigation, plant protection, harvesting, handling, packaging transport are completed through field tools and implements. The cultivation of horticultural crops is predominantly dependent upon human labour, since commercial cultivation is only on a limited scale. So animal/power tiller or tractor-drawn mould board ploughs, disc ploughs, harrows, cultivators and rotavators are available for land preparation and development of new orchards. The fruit plants are generally perennial plants. So different types of sprayers and dusters are available from manually to power operated such as knapsack sprayers foot operated sprayers, power operated mist blowers and dusters or plant protection equipments used for application of insecticides, pesticides and herbicides in the fruit orchards. Some of important implements and sprayers are enlisted below

1. Fruit : Types and their Classification

Fruit:The botanical definition of fruit is a seed-bearing part of a flowering plant or tree that can be eaten as food

or

Fruits are matured ovaries plus any associated flower parts and contain the seeds of the plant. The ovary may be subdivided into two or more carpels (then termed compound ovary) each bearing one to many ovules. Individual carpels develop into sections of a whole fruit, as with citrus where each familiar segment of the fruit represents one matured carpel. If the ovary is not subdivided, then it is termed simple. The ovules will mature into seeds if fertilized. Fruit has three parts.

A. Multiple Choice Questions

1. While studying the history of domestication of various cultivated plants were recognized earlier:
   1. Centers of origin
   2. Centers of domestication
   3. Centres of hybrid
   4. Centres of variation
2. The quickest method of plant breeding is:
   1. Mutation breeding
   2. Hybridization
   3. Selection
   4. Introduction

Biostatistics are the development and application of statistical methods to a wide range of topics in biology. It encompasses the design of biological experiments, the collection and analysis of data from those experiments and the interpretation of the results.

Quantitative traits are polygenic meaning they are controlled by many genes and gene interactions. Examples: height, weight, length etc.

Qualitative character

Qualitative traits are under the controlled of one or few genes, also called oligogeneic traits. For example, black or red coat color, horned or polled, coat color dilution, leaf color are all qualitative traits.

Intellectual Property: Intellectual property is an intangible creation of the human mind, usually expressed or translated into a tangible form that is assigned certain rights of property. It includes, inventions, trademarks, industrial design and geographical indications.

Intellectual Property Rights: Intellectual property rights (IPR) are the rights given to people over the creation of their minds. They usually give the creator an exclusive right over the use of his/her creations for a certain period of time. It includes, creations of the mind: inventions, literary and artistic works, and symbols, names, images, and designs used in commerce.

Marker Assisted Selection (MAS)

Marker Assisted Selection [MAS] is defining as the indirect selection for a desired plant phenotype based on the banding pattern of linked molecular (DNA) markers. It is based to infer the presence of a gene from the presence of a marker which is tightly linked to the gene of interest and high heritability of the gene of interest. Marker assisted selection (MAS) is also known by other names as marker aided selection and marker assisted breeding (MAB).

Molecular markers, including DNA based genetic markers have found their applications in almost all areas of biology, more so in evolutionary biology, genetics and breeding in order to identify a particular sequence of DNA in a pool of unknown DNA. For many applications, DNA markers are preferred over other molecular markers since DNA is stable within an organism over time and among stages of development whereas other molecules are dynamic. A molecular marker is a DNA sequence that is readily detected and whose inheritance can be easily monitored. The uses of molecular markers are based on the naturally occurring DNA polymorphism. Most commonly used molecular markers in biological science include:

1. Restriction Fragment Length Polymorphism (RFLP)

RFLP is the first molecular marker technique which was invented by Botstein et al. (1980). The technique is based on restriction fragment length polymorphism; a molecular marker based on the differential hybridization of cloned DNA to DNA fragments in a sample of restriction enzyme digested DNAs; the marker is specific to a single clone/restriction enzyme combination. It consists of the following steps:

Advances in molecular biology have sharpened the tools of the breeders, and brighten the prospects of confidence to serve the humanity. The application of biotechnology to field crop has already led to the field testing of genetically modified crop plants. Genetically engineered Rice, Maize, Soybean, Cotton, Oilseeds like Rape seed, Sugar Beet and Alfalfa cultivars are expected to be commercialized in the late of 20^th century. Genes from varied organisms may be expected to boost the performance of crops especially with regard to their resistance to biotic and abiotic stresses

Genetic engineering involves isolation of DNA fragments within a cell recombining them outside. The basic technique is quite simple. Two DNA molecules are isolated and cut into fragments by one or more specialized enzymes, the fragments joined together in a desired combination, then restored to a cell from replication and reproduction. This method also referred to as recombinant DNA technology. Genetic engineering developed in the mid 1970 when it becomes possible to cut DNA and to transfer particular pieces of DNA containing specific bits of information, from one type of organism into other.

Tissue culture is a technique used to culture cells, tissues, organs or whole plant under controlled environmental conditions that in vitro condition is known as plant tissue culture. Plant tissue culture exploits the totipotency nature of plant cells, ability to develop in to complete plantlet.

The term ideotype was proposed by Donald in 1968. Ideotype is a biological model which is expected to perform in a predicted manner in a defined environment. A crop ideotype is a plant model that is expected to perform better in a particular environment. The concept of ideotype changes from crop to crop, climate to climate and the concepts and policies of the community concerned. Ideotype breeding is the development of a plant type, expected more quantity of grains, with ideal character. Plant characters are selected from an agronomic point of view.

The prime objective of any plant breeder is to develop high yielding varieties and make the quantity of quality seed of those varieties to farmers for commercial cultivation to realize the benefits of superior varieties. This necessitates, the production of seed of improved varieties in large scale in order to make them available to farmers without losing their genetic purity. Seed production is a continuous process. The seed of improved varieties are produced in several stages, each class producing a special class of seed. The various classes of improved seeds are recognized in order to facilitate seed production and to ensure continuous supply of quality seed. A seed production programme for the production of quality seed has different classes of seeds viz; nucleus seed, breeder seed, foundation seed, registered seed and certified seed. The Association of Official Seed Certifying Agencies (AOSCA) has defined these seed classes as follows:

1. Nucleus Seed

Basic or nucleus seed is the original seed of a released and notified variety. Nucleus seed is multiplied by single plant progeny system, using the concept of maintenance breeding, where purity of the original parental stock is maintained. It is available in very limited quantities and taken from the individual plant of a variety for purification. It is directly under the controlled of concerned crop breeder. This seed has hundred percent genetic and physical purity. Breeder seed is multiplied out of this material and about 500-1000 plants are kept for continued multiplication of nucleus seed.There is no need of any type of tag on nucleus seed but concerned breeder issue a certificate for purity.

Crop breeders are continuously engaged in the improvement and development of crop varieties, in search of a genotype which will prove superior to the existence cultivars. A strain is duly released by appropriate duly empowered legal organization. Only when it is officially released, it is called variety, Before release, it is said to be strain or line. The release of new crop varieties consists of the following major steps:

1. Development of new strains

The new strains are developed by ICAR Crop Research Institutes and State Agricultural Universities for specific purposes. Various breeding methods are used for development of new strains. Some varieties are developed by selection without hybridization such as pure-line variety, mass selected variety and clonal variety. Development of some varieties involves hybridization and selection. This includes varieties that are developed by pedigree, bulk and backcross methods including multiline cultivars and population improvement methods.

2. Evaluation of performance

This trial is conducted at the research station, where new strains has breed. The object is to make sure that new strains are better in performance than the existence variety, so that it can be promoted to the trials where new strains has to be compete with other strains, developed by other scientists/researchers.

The disease , referred as the disorder of crop plants caused by fungi, mycoplasma, bacteria and viruses. Plant resistance is one of the most effective and ideal approach to combat diseases and insect-pest. Resistance is the capacity of plants to resist, withstand and overcome the attack of pathotypes.

Mechanism of Disease Resistance

Different mechanism of disease resistance are as under:

1. Disease escape

The ability of susceptible host plants to avoid attack of disease due to environmental factors like early maturing varieties, shift in the date of planting, change the field, balanced application of NPK etc. Example., early maturing varieties of groundnut and potato may escape from ‘Tikka’ and ‘Late blight’ diseases respectively.

2. Disease tolerance

The ability of the plants to tolerate the attack of the pathogen without loss or little loss in the yield. This endurance is brought about by the influence of external characters. Generally, tolerance is difficult to measure since it is confounded with partial resistance and disease escape. Disease tolerance is of three types:

Abiotic stresses include salinity, drought, flood, extremes in temperature, heavy metals, radiation etc. They are the foremost factors that causes the loss of major crop plants worldwide. This situation is going to be more rigorous due to increasing desertification of world’s terrestrial area, increasing salinization of soil and water, shortage of water resources and environmental pollution.

The inadequacy of water availability, including precipitation and soil moisture storage capacity, in quantity and distribution during the life cycle of a crop to restrict the expression of its full genetic yield potential”. Generally drought stress occurs when the available water in the soil is reduced and atmospheric conditions cause continuous loss of water by transpiration or evaporation. Drought stress tolerance is seen in almost all plants but its extent varies from species to species and even within species.

Polyploidy, the condition in which a normal diploid cell or organism acquires one or more additional sets of chromosomes. In other words, the polyploid cell or organism has three or more times the haploid chromosome number. Polyploidy arises as the result of total nondisjunction of chromosomes during mitosis or meiosis. The somatic chromosome number of any species, whether diploid or polyploidy is designated as 2n. The somatic cells of plants are diploid that they posses two complete set of chromosomes, one received from seed parent and another from pollen parent. The gametic chromosome number is said to be haploid and denoted by n. Haploid is defined as cell with gametic chromosome number. While monoploid has the basic chromosome number and represented by x.

Classification of Polyploidy

Polyploids are classified in to two groups, euploidy and aneuploidy. Euploidy have chromosome number which is an exact multiple of basic number. Euploidy includes monoploids, diploids and polyploids. In aneuplod individual with other than exact multiple of basic chromosome number or change in chromosome number which posses one or few chromosomes of the genome.

Mutagenesis is the process whereby sudden heritable changes occur in the genetic information of an organism not caused by genetic segregation or genetic recombination, but induced by chemical, physical or biological agents.

Mutations were first identified by Hugo de Vries in the late nineteenth century, while experimenting on the ‘rediscovery’ of Mendel’s laws of inheritance. He coined the term ‘mutation’. Radiation-induced mutations as a tool for generating novel genetic variability in plants, advanced as a field after the discovery of the mutagenic action of X-rays demonstrated in maize, barley and wheat by Stadler. The discovery of X-rays by Rontgen in 1895 led to the application of X-rays for inducing mutations in fruit fly by Muller (1927). Mutation is of two types

Reproduction that does not involve the union of male and female gametes is known as asexual reproduction. Reproduction in which sexual organs or related structures take part but fertilization does not occur, or crops which are propagated asexually or by vegetative means are known as asexually propagated crops. Most of the fruit plants are propagated asexually which consist of large number of clones. Example: sugarcane, potato, sweet potato, banana, mango, citrus, pears, peaches, litchi, crops that propagates by asexual means. The procedure of selection used for asexually propagated crops is known as clonal selection, since the selected plants are used to produce new clones or superior clones can be isolated from local variety, introduced variety or inter crossed population.

Hybrids are produced by crossing two genetically diverse parents variety. Hybrid vigour is at its maximum in the F₁ generation and hence highest emphasis is placed on the F₁ hybrids. Hybrid varieties leads to maximum exploition of heterosis as compared to any other varieties. Beal in 1880 recommended the use of F₁ varietal crosses of maize for commercial cultivation.

The commercial exploitation of heterosis started only after the rediscovery of Mendel’s law of inheritance and consequent understanding of the theoretical basis of hybrid vigour. Heterosis is considered as a major revolution in classical plant breeding era. Utilization of heterosis has substantial influence in genetics to provide hybrid vigour, consequently high yielding hybrids in different crops and plants. Yield increase from heterotic hybrids due to the expression of heterosis is up to 30% superior to conventional varieties. The term heterosis was first used by Shull (1914) and represents the increase or decrease in fitness and vigour of the F₁ hybrid over the mean or mid parent values, produced by crossing two genetically different individuals for one or more traits. Generally, positive heterosis is desirable but in some cases like maturity duration and presence of toxic substances, negative heterosis is condiderable as desirable. Heterosis can be estimated in three different ways. When the heterosis is estimated over mid parent, it is called as relative heterosis or mid parent heterosis. Estimation of heterosis over better parent out of the two parents involved in the cross is referred as “Heterobeltiosis”, the term which was coined by Fonseca and Patterson (1968). “Standard Heterosis”, often called as useful heterosis which was coined by Meredith and Bridge (1972) and denotes the superiority of F₁ over the standard commercial check.

1. Bread Wheat (Triticum aestivum)

Wheat has been traditionally grouped into the genera Triticum in the tribe Tritiaceae of the family Poaceae. Bread wheat, 2n=6x=42 is a hexaploid consisting of three distinct genomic complementary, namely, A, B and D, with the genome structure AABBDD. In the past there were 4 species, viz. Triticum aestivum, T. durum, T. dicoccum and T. sphaerococcum, under cultivation in India. T. sphaerococcum has now practically gone out of cultivation because of its low productivity and high susceptibility to diseases. The major wheat producing countries are China, India, USA, the Russian Federation, and Australia. These five countries together contribute more than half of the global wheat production. In India, Uttar Pradesh has registered the highest production followed by Punjab, Madhya Pradesh, Haryana, Rajasthan and Bihar. These top six states together contributed around 90 per cent of the total wheat production in the country

Floral Biology

The inflorescence of wheat is known as spikelets. Spikelets are alternately arranged on the rachis; each spikelet’s has a small joint axis which bear two to nine flower known as florets. Close to the base of rachilla are two empty boat shaped bracts known as glumes. Above these inserted alternately on opposite side of the rachilla known as lemma. Opposite it’s a thin membrane bracts known as palea enclosed within the lemma and palea are a single pistil and three steamens. The pistil consists of an ovary bearing two short styles each carrying a feathery stigma. At the base of the ovary are two membranous scales known as lodicules. Each stamen consists of a thread like filament carrying a pollen containing anther.

The genetic organization of cross pollinated crops are different from that of self pollinated crops, because of differences in the reproductive system. Open pollinated populations are heterozygous with free inter mating individuals, heterozygous at most of the loci. Heterozygosity is an essential feature of commercial varieties of out breeders. It is maintained during breeding or is restored at the end. Population improvement involves selection, generation after generation, with recombination of selected individuals. Its aim to increase the frequency of favorable alleles in the population. The improved forms may be used for commercial cultivation or as a base germplasm for the derivation of inbred lines. The list of cross pollinated plants is presented in Table 10.1. The factors that promote the cross pollination are dicliny, dichogamy, herkogamy, self-incompatibility and male sterility. The breeding methods which are commonly used in cross-pollinated crops are population improvement, hybrid and synthetic varieties.

Population Improvement

In cross pollinated crops heterozygosity must be restored in the end product of any breeding programme. It is classified into the following types:

The prime object of plant breeder is to develop high yielding variety with superior characteristics. Selection is based on the phenotypes of the plants. Consequently the effectiveness of selection primarily depends on the degree to which the phenotype of plants reflects their genotype. These are all self fertilizing species. In these species development of seed take place by self fertilization. Hence self pollinated species are also known as autogamous species. Various plant characters such as homogamy, cleistogamy, chasmogamy, bisexuality etc. promotes self pollination. The self pollinated crops with their description are given in Table 9.1. The breeding methods used in self and often-self pollinated crops are discussed below:

Plant Introduction

Plant introduction consists of taking a genotype or a group of genotypes of plants into a new area or region or environment, where they have not being grown earlier. It is very simplest and fastest method of developing new variety. It is of two types:

The mating or crossing between two genetically dissimilar plants or lines is known as hybridization. In other words, hybridization is an artificial controlled pollination in which pollen grains of desired male parent is transferred on the stigma of the desired female parent.

Objectives of Hybridization

To create genetic variability, which is pre-requisite for crop improvement programme.

To transfer one or more characters into single plant.

To know the inheritance of the characters.

To estimate the general combining ability of the parents and specific combining ability of the crosses.

To exploit and utilize the hybrid vigour.

To produce hybrid/synthetic varieties.

Pollination can be defined as the pre-fertilization event or process where pollen grains from anther are transferred to the stigma of a flower. Pollination is the process that helps to unite the male and female gametes and thus helps in fertilization. It can be broadly classified into two, cross-pollination and self-pollination.

1. Self-Pollination

Self-pollination is the process by transferring the pollen grains directly from anther into the stigma of the same flower. It is called as self-pollination. This form of pollination is common in hermaphrodite or dioecious plants which have both male and female sexual organs on the same flower. In self-pollinating flowers, the anthers and stigma are of similar lengths to facilitate the transfer of pollen. Self – pollination can be further divided into two types:

Self incompatibility in plants is a mechanism preventing the seed set by self pollination, and is probably way in which out crossing is enforced. Other factors may cause selfed seed may not to set seed due to embryo abortion, but SI is prezygotic and prevents embryo formation. SI is, therefore prevention of fusion of fertile male and female gametes after fertilization. Koelreuter first time in 18^th century reported S.I in Verbascum phoeniceum plants. In self-incompatible pollen grains fail to germinate on the stigma of the flower that produced them. Self incompatibility has been reported in more than 300 plant species belonging to 70 family of angiosperm. It is important out breeding device (cross pollination) for normal fruit set. It is maintained high degree of heterozygosity and can take place at any stage between pollination and fertilization.

Male sterility refers to a condition in which pollen is either absent or non functional or unable to produce functionally viable pollen in flowering plants. Since it was first reported by Kolreuter as early in 1763 that anther abortion occurs naturally in some plant species or in their inter specific crosses, its occurrence was observed in several species. In the 19th century, Gartner (1844) and Herbert (1847) reported the occasional recurrence of anthers rudimentariness or abortion in many ornamental and vegetable crops in normal populations. During the early 20th century, a large number of species were shown to possess male sterility, for example, Suturela hortensis, Lathyrus odoratus, Merculalis annua, Solanum tuberosum, Vitis vinifera, Linnum usitatissimum, Zea mays, Antirrhinum majus, Prunus persicaer and Rubus idaeus. Following the rediscovery of Mendel’s Laws of Inheritance, study of the genetic main basis of inheritance of male sterility attracted the attention of several workers. The main impetus for the study of inheritance of male sterility came from the elucidation of heterosis in maize by G. H. Shull, in 1908 and its exploitation through the use of male sterility by Jones and Clark (1943). The male sterility based scheme of hybrid seed production in corn opened up a new era for the hybrid corn industry as it could overcome the necessity of detasseling of female (parent) lines in the hybrid seed production process.

The male sterility may arise due to malformation of male sex organs, lack of normal anther sac, and inability of anthers to release mature pollens, lack of development of normal microspore.

Apomixis is an asexual method of reproduction through the seed. Apomictic reproduction is widespread and found in over 300 species of plants in 30 families. Interest in apomixis as a reproductive mechanism has increased as its potential and role are being recognized in genome preservation, polyploid build up, propagation of derivatives of wide crosses, permanent fixation of hybrid vigour, evolution, speciation and plant breeding .

Winkler (1906) defined apomixis as “the substitution for sexual reproduction of another asexual reproductive process that does not involve nuclear or cellular fusion.” One of the chief barriers to an understanding of the apomictic phenomenon has been the highly complex terminology as well as the differences of opinion among specialists as to the correct usage of these terms, probably because the phenomenon by itself is complex and in many ways quite different from that of amphimixis. Convincing evidence for the presence of apomixis can be obtained by tracing the steps that are involved in embryonic development. It should be amply clear that as the prerequisite for apomixis, fertilization does not take place at all in any form. Thus there is no normal development of embryo from the egg cell. However, the embryo may develop from an unfertilized egg cell other than the egg cell within the embryo sac.

Apomixis is widely distributed among higher plants. More than 300 species belonging to 35 families are apomictic. It is most common in Gramineae, Compositae, Rosaceae and Rutaceae. Among the major cereals like maize, wheat and pearl millet have apomictic relatives.

Germplasm is the sum total of all the genes and their alleles present in a crop and its related species. Germplasm is also termed as genetic resources. It may be release as a variety, subjected to selection for developing a variety, and may be used as a parent in hybridization programme. They may be obtained from gene banks, center of diversity, gene sanctuary, farmer's field and seed companies.

Types of Germplasm

1. Land races

These are primitive varieties/cultivars. They are storehouse of genetic variability. Land races were first collected and studies by N.I.Vavilov in rice. They are the source of development of varieties in many valuable crops.

2. Obsolete varieties

The improved varieties of recent past are called as obsolete varieties. These varieties were breed by systematic breeding methods, were once commercially cultivated, but are no more grown. For example, wheat varieties K65, Ph591, many NP series etc.

Centre of origin are also known as centre of diversity. N. I. Vavilov in 1926 has proposed that crop plants evolved from wild species in the areas showing diversity and termed them as primary centers of origin. Primary centers, crops moved to other areas with the movements of man. But in some areas, certain crop species show considerable diversity of forms although they did not originate there. Such areas are known as secondary centers of origin of these species. Genetic diversity is essential for crop improvement. Vavilov has suggested eight main centers of origin of crop plants.

1. Chinese Centre

This centre is considered to be one of the earliest and largest independent center of origin of cultivated plants. This centre includes mountain regions of central and western China. The endemic species listed from this centre include, soya bean, radish, turnip, pear, peach, plum, colocasia, buckwheat, opium poppy, brinjal, apricots, oranges, china tea etc.

2. Himalayan Centre

This centre is also known as the Indian centre of origin. This centre includes regions of Assam, Burma, Indo-china and Malayan Archipelago. The crops originated from this centre are rice, red gram, chick pea, pea, mung, brinjal, cucumber, sugar cane, black pepper, moth bean, rice bean, cotton, turmeric, indigo, millets etc.

Plant breeding is the art and science of changing and improving the heredity of plants. The art of plant breeding lies in the breeder’s skill in observing and selection of superior plants. The value of selection largely depends upon the skill of the breeder. The skill that a breeder develops though a close and deep study of the concerned crop species. Before the rediscovery of the Mendelian Laws in 1900 AD, the plant breeding is as an art. The modern age of plant breeding began in the early part of the twentieth century, after Mendel’s work was rediscovered. Today plant breeding is a specialized technology based on genetics. It is now clearly understood that within a given environment, crop improvement has to be achieved through superior heredity. Plant breeding developed into a science as knowledge progressed towards the genetics, cytogenetic, physiology, pathology, biochemistry, biotechnology and biometry. The fundamental of plant breeding was based on recognition of the genes. The genes were identified by their effects on the visible expression of plant traits such as whether a plant was a dwarf or tall, or the flower color was white or pink. Through systematic hybridization, a desirable gene could be transferred from one to another cultivar. More recently the science of molecular genetics proposes to advance plant breeding. Molecular genetics was ushered in with the description of chemical structure of deoxy-ribonucleic acid (DNA), the material that constitutes the genes. DNA carries the instructions for synthesis of specific proteins that determine the visible expression of particular characters. The new technology does not supplant nor diminish the importance or need for traditional selection and crossing procedures in the breeding of improved cultivars. The performance of today’s cultivars reached at high levels through refined selection and hybridization breeding procedure.

The early developments in plant breeding that took place are based on archaeological evidence. The first efforts to grow wild plants probably occurred in Southeast Asia culminated in the domestication of some of the many plants with which humans experimented in 13000 BC. By 10000 BC, advanced knowledge existed of such plants as rice (Oryza sativa), several legumes such as pea (Pisum sativum), runner bean or broad bean (Phaseolus or Vicia spp.) and possibly soybean (Glycine max). At this point crops became domesticated.

Although the beginning of civilization in China and Southeast Asia are obscure and fragmented. The number of successful species domesticated indicates considerable success in plant breeding. The third site of independent agricultural development and plant breeding was in south-central Mexico between 6700 and 5000 BC. Squash and avocados were domesticated first and a number of species of maize. Domestication in the Middle East and China included the self-fertilized crop like cereals and soybean.

Meaning – Points to be considered in public speaking – Effective public speaking: Group Discussion: Meaning – Procedure – Advantages – Limitations; Seminar Conferences: Definition and meaning – Steps in organizing seminar / conferences/ symposium / workshop

Definition

Public speaking is the process and act of speaking or giving a lecture to a group of people in a structured, deliberate manner intended to inform, influence, or entertain a listening audience. Public speaking is commonly understood as face-to-face speaking between individuals and an audience for the purpose of communication. It is closely allied to “presenting”, although the latter is more often associated with commercial activity. Most of the time, public speaking is to persuade the audience.

Public speaking can serve the purpose of transmitting information, telling a story, motivating people to act or some combination of those. This type of speech is deliberately structured with three general purposes: to inform, to persuade and to entertain. Knowing when public speaking is most effective and how it is done properly is a key part in understanding the importance of it.

Points to be considered in public speaking

1. Prepare to succeed

The single most important thing you can do to make your next presentation successful is to be prepared. That means make time for some serious practice. Experts suggest you spend 60% of your time to prepare your content and slides and 40% of your time to practice.

2. Say it correctly

Part of good preparation is double-checking the pronunciation of any tricky words—especially jargon. Your correct pronunciation and knowledge of industry terms lends credibility to your presentation.

Meaning-Steps for individual presentation; Group presentation-Meaning-Stages of group presentation; Impromptu presentation.

A Presentation is the process of presenting a topic to an audience. It is typically a demonstration, introduction, lecture, or speech meant to inform, persuade, inspire, motivate, or to build good will or to present a new idea or product.

Individual Presentations allow a person to completely control the thoughts, ideas, and methods of presenting without outside influence. Individual presentations allow someone to work at their own leisure and pace.

The practice of showing and explaining the content of a topic to an audience or learner is known as presentation. A presentation is a process of offering for consideration or display.

Presentations can also be categorized as expository or persuasive. They can also be impromptu, extemporaneous, written, or memorizes. But it’s more important to focus on their purpose. There are three basic purposes for giving presentations:

Definition-Purpose of abstract-Types of abstract-Abstract Styles-Steps for Writing Effective Abstracts- Some Do’s Don’ts in preparing abstracts.

Definitions

The word abstract comes from the Latin word abstractum, which means a condensed form of a longer piece of writing. Abstracts are important parts of reports and research papers and sometimes academic assignments. The abstract is often the last item that you write, but the first thing people read when they want to have a quick overview of the whole paper. We suggest you leave writing the abstract to the end, because you will have a clearer picture of all your findings and conclusions.

An abstract is a paragraph that provides an overview of a paper. Abstracts should be between 100 and 200 words long and usually contain a list of keywords at the end to help readers identify the main points of the paper. While abstract requirements differ across academic disciplines, there are two main types of abstracts: humanities abstracts and scientific abstracts.

Purpose of abstracts?

Abstracts typically serve five main goals: