eChapters

Fig 8.1: A scheme for fertigation program working with automation

In modern farming with fertigation machines (NutriCare) and automated drip irrigation systems, fertigation set up schemes like the above are put into practice. This scheme will stream line the installation, operation and monitoring of the entire process. An informed grower would be able to control crop performance more precisely and achieve his objectives.

The human body consists of trillions of cells and to be healthy it needs variety of foods and nutrients. The food around the globe includes a variety of spices which have many health benefits. It has been found that spices and vegetables in the diet are very important for living healthy. The traditional foods, nuts, seeds, wild fruits and vegetables which are used in the diet are grown in specific areas. Traditional food system plays a significant role towards maintaining the well-being and the health of people in any specific region.

According to Indian Philosophy (Bhat, 2012) the physical body is called Annamaya Kosha.A body, when well nourished grows well by absorbing the energies from the food consumed. Fasting is another aspect of traditional food system. It is meant to purify the mind and the body.

We all eat food because it provides the nutrients that are needed for the various functions of the body. Energy is required for growth and repair of tissues, to maintain body temperature and for physical activity. Energy comes from foods which are rich in carbohydrates, proteins and fat. This provides energy to the body from other nutrients and helps to keep the skin and nervous system healthy. They help towards the various biological activity in the human body. These nutrients are grouped into carbohydrates, proteins, lipids (fat), water, vitamins and minerals. These are used for growth, maintenance, repair and other needs of the body. Protective foods are which contain adequate amounts of vitamins, minerals and high-quality proteins which protect against the development of a deficiency disease. There are various chemical reactions taking place within each cell of a living organism. These provide energy for vital processes and for synthesizing new organic materials. This process is known as Metabolism. Living organisms get energy from the environment and use it for movement, growth, development and reproduction.

Bacteriochlorophylls: A group of light absorbing photosynthetic green pigments of anoxygenic photosynthetic bacteria.

Ballistic transformation: The use of a particle gun to insert foreign DNA into a host.

Beneficial elements: These are the elements that help to optimize the growth and development only in some species. It is not essential to all the plants. Aluminum is beneficial to some species that are adapted to acidic soils. Cobalt can have a number of beneficial effects, especially in leguminous plants.

Betalains: Highly coloured nitrogen containing glycosylated compound in the category of alkaloids imparting red-purple and yellow colour in plants of centropsermae (e.g., beet root).

Beta-oxidation: Breakdown of fatty acids into Acetyl co-A by successive oxidation. It is the first step of lipids breakdown.

Bidirectional transport: Simultaneous transport of solutes in opposite directions in the same file of sieve elements. Bidirectional transport may occur under some circumstances. For e.g., in a file of cell, as a result of cytoplasmic streaming.

Ice nucleation: It is a process of reducing the formation of ice crystal under freezing stress, by some polysaccharides and proteins known as ice nucleators.

Imbibitions: It is a special type of diffusion when water is absorbed by solids-colloids-causing them to enormously increase in volume. The classical examples of imbibition are absorption of water by seeds and dry wood.

Immobile elements: The elements which get translocated slower inside the plant are called immobile elements. Examples: Ca, Fe and B.

Immunoassay: This is very sensitive method of identifying and or quantifying the hormones and other molecules which has antigen and antibody interaction.

Indeterminate growth: Plant growth in which the main stem continues to elongate indefinitely without being limited by a terminal inflorescence or other reproductive structure; also growth is characterized by sequential flowering from the lateral or basal buds to the central or uppermost buds.

Indole -3 acetic acid: It is naturally occurring auxin responsible for cell elongation, apical dominance and other physiological functions of auxins.

Induced dormancy: Induced dormancy in which dormancy develops as a response to external environmental factors.

Vacuole: A fluid filled space within the cytoplasm surrounded by membrane called as tonoplast, that occupies most of the volume of cell. Aside from storage, the main role of the central vacuole is to maintain turgor pressure against the cell wall.

Valinomycin: An antibiotic that uncouples electron transport and oxidative phosphorylation only in presence of some cations such as K⁺ or Na⁺

Van der Waals interaction: Weak intermolecular forces between molecules as a result of each inducing polarization in the other.

Wall pressure: The inward pressure exerted by the cell wall against expanding protoplast; equal but opposite to turgor pressure.

Warburg effect: Warburg effect states that the competition between CO₂ and O₂ for enzyme RuBisCO with CO₂ promoting photosynthesis and inhibiting glycolate formation and inhibiting photorespiration and O₂ promoting glycolate formation and inhibiting photosynthesis.

Water deficit: Water available to the plant is less than optimum requirement.

Water potential (ψ_w): Water potential is defined as free energy status of water in a system compared to the free energy status of pure water. Here system refers to water in plant cell or soil and pure water refers to the water which does not have any solute in it. Water potential is measured in terms of bars, Pascals or Megapascals and denoted by ψ_w.

The species is commonly found in tanks, ponds, lakes, reservoirs, canals, swamps, etc in freshwater plains. It is air-breathing in nature and survives easily low oxygen stress. During drying-up of water bodies in summers, it has been reported to penetrate deep under moist-earth and come out live on arrival of rains. The name 'murrel' encompasses its resemblance to reptile (snake) due to presence of body scales all over its body including head region. Fully-grown adults are dark greenish brown with faint black and white vertical bands visible across its entire abdominal length below the lateral line and a black ocellus at posterior end of dorsal fin.

Striped murrel is one of the important food fish of high commercial value due to preference for its meat quality, therapeutic and antinociceptive properties and hence consumed by many to induce healing and pain control after clinical operations. It contains high arachidonic acid (AA), which is a precursor of prostaglandin that may initiate blood clotting and enhance tissue growth. Geographically, the species is distributed in Western to Southeast Asia including Bangladesh, Cambodia, China, India, Indonesia, Lao People’s Democratic Republic, Malaysia, Myanmar, Nepal, Pakistan, SriLanka, Thailand and Vietnam (Chaudhary, 2019). It is supposed to be introduced in countries like Fiji, New Caledonia (Welcomme, 1988), Madagascar (Stiassny and Raminosoa, 1994) and Hawaii (Courtenay et al., 2004). The species has been reported to grow to a length of 100 cm (Rahman, 1989) and categorized„ Least Concern under the IUCN Red List (IUCN, 2020).

Globally, 92523 ton of striped murrel is produced (FAO, 2016), of which the major share of 70802 ton is contributed from capture fisheries and 21721 ton from aquaculture (Figure 1). Though capture fisheries of striped murrel is quite stable in terms of global production, the aquaculture has shown a steady rise in last two decades. In India, though no production data is available, the production of this species is mainly contributed from the capture fisheries with meager contribution from aquaculture. This has caused serious stress in past few years on its resources, more particularly due to habitat degradation and man-made hazards in aquatic ecosystem.

3.1 Spawning Season

Striped murrel breeds in stagnant waters and commence breeding in ditches, ponds, tanks, flooded paddy fields, wetlands and dead end of rivers; a little prior to or with the onset of monsoons (Bhattacharya 1946; Chacko 1947). Striped murrel is a batch spawner species and hence breeds several times in a year. According to Courtenay et al. (2004), spawning occurs in this species all through the year. In northern states of India, the breeding season of this species coincides with south-west monsoon and extends from June to September with peak in mid of June to mid of August (Qasim and Qayyum, 1961). However, the species has been reported to breed during November to January in southern India (Alikunhi, 1953).

The inoculum dosage and method of application determines the efficacy of biofertilizers. The quantity of biofertilizers to be applied depends on the type of crop, seed rate and soil edaphic factors. There are 3 methods for inoculation of biofertilizers

Seed treatment: One packet containing inoculant (200 g) is mixed with 200 ml of rice gruel or 40 % gum arabic solution to make a slurry (Fig. 23). Then the seeds required for an acre are mixed in the slurry in such a manner that they have a uniform coating of the inoculant over the seeds. Then, the inoculated seeds are shade dried for 30 minutes. The shade dried seeds should be sown within 24 hours. One packet of the inoculant (200 g) is normally sufficient to treat 10 kg of seeds.

It has been well established that the biofertilizers are beneficial to plants and are cost-effective inputs for sustainable production system. However, there are some constraints with respect to its production, which are presented below:

10.1 Production constraints

10.1.1 Raw material

Eventhough there are large number of biofertilizer formulations, only few are considered as good carrier materials for biofertilizers such as peat and lignite. Moreover, its availability in India is limited and the quality is lacking for these carrier materials. Hence, a cost effective and locally available material with increased shelf-life is the need of the hour.

10.1.2 Specificity and availability of strains

As the microbial strains used as biofertilizers are specific to soil and agro-ecological conditions, availability of specific strains for the specific host and soil edaphic factors are inadequate.

Fungi are achlorophyllus so they have to depend on other organisms hence heterotrophic nutrition. Depending on the nutrition, fungi are classified as Saprophytes, Parasites and Symbionts.

3.1 Saprophyte (sapros = rotten; phyton = plant)

Fungi which get their nutrition only from dead organic are called saprophytes ex: Saprolegnia, Rhizopus, Mucor, Alternaria etc.

3.1.1 Facultative saprophyte (Facultas = ability)

Ability of a fungus to become saprophyte. It is a parasite ex. Ustilago

3.1.2 Obligate saprophyte (obligate = to bind itself)

When they strictly get their food only from dead organic matter and can never become parasites are called obligate saprophyte ex. Mucor.

Plant diseases are classified on the basis of type of pathogenic or non-pathogenic causes of the disease. The classification is based on the plant pathogenic organisms as follows.

A. Parasites

The diseases are incited by parasites under a set of suitable environment. Association of definite pathogen is essential with each disease. They include both biotic and mesobiotic agents.

Biotic agents: They are also called as animate causes. They are living organisms.

PRACTICAL 1: PREPARATION OF READY-TO-COOK MUSHROOM PASTA

Pasta is a product originally made from wheat and is of different shapes and sizes. It is generally rich in carbohydrate but is deficient in protein especially amino acid balance. Therefore, addition of spices and dried vegetables may solve this problem. Moreover, fortification of pasta with tomato, garlic, mushroom or spinach makes it nutritious for consumption. Since instant foods are widely becoming popular and consumed throughout the world and is also a fast growing sector of the noodle industry because of busy life schedule of people.

EXPERIMENT: MINIMAL PROCESSING OF CAULIFLOWER (FREEZING PRESERVATION AND STEEPING PRESERVATION)

Materials and reagents

Material : Fresh cauliflower, glass jars, polythene pouches

Reagents: 15% sodium chloride

6.1 Introduction

Garden design encompasses the aesthetic beauty, artistry, knowledge of plant growth and development with basic maintenance of plants and other elements as required. Principles are the basic rules of designing a landscape. The basic principles of garden design are very simple; however, each is often referred to by more than one name. Principles of landscape garden keeps the garden lively and beautiful. The principles of gardening should be kept in mind always while designing a garden.

6.2 Axis

It is an imaginary line in landscape designs around which the garden is created striking a balance. In a formal garden the central line is the axis.

5.1 Purpose

To employ a standard protocol to perform agarose gel electrophoresis.

5.2 Principle

Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb. Migration rate of DNA molecule depends on various factors like 1) size of DNA molecule; 2) agarose concentration; 3) DNA conformation 4) voltage applied, 5) presence of ethidium bromide, 6) type of agarose and 7) electrophoresis buffer. After separation, the DNA molecules can be visualized under UV light.

The prime objective of any plant breeder is to develop high yielding varieties and make the quantity of quality seed of those varieties to farmers for commercial cultivation to realize the benefits of superior varieties. This necessitates, the production of seed of improved varieties in large scale in order to make them available to farmers without losing their genetic purity. Seed production is a continuous process. The seed of improved varieties are produced in several stages, each class producing a special class of seed. The various classes of improved seeds are recognized in order to facilitate seed production and to ensure continuous supply of quality seed. A seed production programme for the production of quality seed has different classes of seeds viz; nucleus seed, breeder seed, foundation seed, registered seed and certified seed. The Association of Official Seed Certifying Agencies (AOSCA) has defined these seed classes as follows:

1. Nucleus Seed

Basic or nucleus seed is the original seed of a released and notified variety. Nucleus seed is multiplied by single plant progeny system, using the concept of maintenance breeding, where purity of the original parental stock is maintained. It is available in very limited quantities and taken from the individual plant of a variety for purification. It is directly under the controlled of concerned crop breeder. This seed has hundred percent genetic and physical purity. Breeder seed is multiplied out of this material and about 500-1000 plants are kept for continued multiplication of nucleus seed.There is no need of any type of tag on nucleus seed but concerned breeder issue a certificate for purity.

Characters

Two orders i.e. Archaeognatha & Thysanura are grouped together as Ectognatha because the mouth parts are more or less exposed and not covered by cranial fold.

Health is defined as a state

a) Where all body system function normally

b) Absence of disease

c) Activities continue within normal physiological limits

d) All of the above

1 Inflammation of peritonium is called

a) Peronitis b) Peritonitis

c) Laminitis d) Oophonitis

1 Delayed post natal diseases occurs within _______ days after birth

a) 10 days b) 2-7 days

c) 2 days d) 15-30 days

1. ICAR-National Research Centre for Banana, Trichi

2. ICAR-National Research Centre for Grapes, Pune

3. ICAR-National Research Centre for Litchi, Muzaffarpur

1. ICAR-National Bureau of Plant Genetics Resources, New Delhi

2. ICAR-National Bureau of Agriculturally Important Micro-organisms, Mau, Uttar Pradesh

Directorates/Project Directorates

1. ICAR-Directorate of Groundnut Research, Junagarh, Gujarat

2. ICAR-Directorate of Soybean Research, Indore, Madhya Pradesh

Agricultural Research Centers in World

1. Consultative Group on International Agricultural Research (CGIAR Centers)- Montpellier, France (1971)

2. International Center For Tropical Agriculture (CIAT)-Colombia (1969)

Aim of the Experiment: The composite soil samples, collected, are air-dried and processed before analysis in the laboratory.

Pore space or porosity : Porosity is an index of the relative pore volume in the soil or volume fraction of pores. Its value generally lies in the range of 0.3-0.36 (30-60%). Coarse textured soils tend to be less porus than fine textured soil, though the mean size of individual pores is greater in the former than in the latter. The total porosity, in any case, reveals nothing about the pore size distribution which itself is an important property.

Primary particles tend to adhere under natural condition so as to form secondary unit called aggregates. It is very difficult to determine the diameter of the individual aggregated particle. So the mean weight diameter is calculated as a single value constant, which indicate the wetted average value for a group of aggregates.

The soil water plays an important role in the physical, chemical and biological activities taken place in the soil which directly or indirectly affects the crop growth. The water content of the soil can be measured by different methods. These methods are gravimetric method, neutron scattering method and electrical resistance method.

Field capacity may be defined as the amount of water held in soil after excess water has drained away and the rate of downward movement of water was materially decreased which usually taken place within two to three days after heavy rain or irrigation. In this condition water was moved out of the macropores and air has moved into take its place. 

Infiltration is the entry of a fluid from one medium into another. Soil water plays an important role in nature. The entry of rain or irrigation water into soil is called infiltration. Infiltration rate is defined as the amount of water entering into a soil per unit area per unit time under one atmospheric pressure. The infiltration rate depends upon initial moisture content of soil, condition of soil surface and permeability of various layers.

The maximum water holding capacity is the amount of water, expressed as percentage on oven dry basis held by a soil when it is completely saturated with water. At this moisture content, all the pore spaces are completely filled with water.

The lime requirement (LR) of an acid soil is the amount of lime needed to neutralize the acidity from an initial condition to some target soil pH, like 7.0. Different methods are used to calculate a LR for specific soils. Lime requirement tests will always recommend adding enough lime to neutralize all the acidity associated with soil pH, much of the salt-replaceable acidity, and some of the residual acidity.

The statement is still valid as long as it is understood that in an aqueous solution the substances involved are subject to dissociation, which changes the ionization state of the substances. 

The Kjeldahl method is mainly divided into three main steps. This method needs to be carried out in proper sequence. 

Olsen P were found to bear a quadratic relationship, with Olsen’s extractant underestimating the content in phytoavailable P of soils with high Olsen P contents relatively to soils with low contents. The ‘‘change point’’ at which phytoavailable P began to increase rapidly per unit change in Olsen P was 53 mg Olsen P kg–1 soil. For the acid soils, a significant quadratic relationship was found between the amount of P desorbed to water and Olsen P at the three soil:solution ratios studied.

Potassium is extracted from the soil with the help of suitable extractant CH3COONH4 by shacking followed by filtration or centrifugation & is determined in the extract using flame photometer. The analysis photometer is based on the measurement of the intensity of characteristic lime emission given by the element to be determined .

Reagents

• Buffer solution : Dissolve 67.5 g of Ammo. Chloride in 200 ml distilled water. Add 570 ml Ammonium solution. The volume make up to 1 ltr.

• EBT : 0.2 g in 25 ml methanol.

Soil is shaken with 0.15 % CaCl2 solution. Chloride ions displace adsorbed sulphate during extraction. Calcium ions suppress the extraction of soil organic matter and hence eliminate the contamination caused by extractable organic S.

Procedure

• Take 1 g soil in a 50 ml beaker

• Add 25 ml 1M KCL solution 

Take 1 g phenolpathalin powder in a 100 ml volumetric flask. Then add volume up to 100 ml by ethanol (Ethyl alcohol).

Digestion Mixure : This is a mixture of two salt. That is potassium sulfate and cupper sulfate with 9:1 ratio (90 g Potassium sulfate + 10 g Copper sulphate).

100 ppm ‘P’ : By dissolving oven dry KH2 PO4 salt. Take 0.4393 gm of KH2 PO4 and volume to 1 ltr that gives 100 ppm ‘P’.

Take 5 ml/2.5 ml aliquot in a 25 ml volumetric flask.

6.25 HNO3 (for 2 ltr) : Take 181 ml of conc HNO3 and diluted to 2 ltr in a volumetric flask.

Aim : To determine the Calcium in plant sample.

Aim : To determine the Magnesium in plant sample.

Kerria japonica var. pleniflora Witte. (Rosaceae): Eng.- Jew’s Mallow / Japanese Rose [NI]. A deciduous ornamental shrub with vigorous and upright green shoots; alternate, ovate, pointed, sharply toothed, bright green leaves; produces very attractive, solitary, pompon like, double yellow flowers in April.