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Clinical conditions similar to Brucellosis have been described since the time of Hippocrates (450 BC). J. A. Marston first described brucellosis as gastric remittent fever in 1861 at Malta (Marston, 1861). Sir David Bruce in 1886 first isolated little coccobacillus from an infected soldier’s spleen. However he isolated the bacteria in a pure culture in 1887 and named Micrococcus melitensis (Bruce, 1889). Later the genus was renamed as Brucella in his honour. Similarly, Bang and Stribolg first isolated Brucella abortus from abortion material of a cow (Bang, 1897). Traum in 1914 isolated the Bacillus abortus suis. Presently we know it as Brucella suis. In 1953, Brucella ovis was first isolated in New Zealand (Budlle and Boyes, 1953) from the aborted material of sheep. Carmichael and Bruner in 1968 first isolated B. canis from the aborted placenta of a dog.

            In India first report of ‘Contagious abortion’ by Brucella was found from annual report of Imperial Veterinary Research Institute (presently Indian Veterinary Research Institute), Mukteswar, Uttarakhand (Anonymous, 1918). Seroprevalence of Brucellosis is reported from various states of India afterward (Polding, 1942; Sethi et al., 1971 etc.).

Brucella species are small (0.6 x 0.6 to 1.5 pm), nonmotile, coccobacillary, Gram-negative bacteria. As they are not decolourized by 0.5% acetic acid in the modified Ziehl-Neelsen (MZN) staining technique, they are classed as MZN-positive. In MZN-stained smears of body fluids or tissues, they characteristically appear as clusters of red coccobacilli. For taxonomic purposes, all Brucella species should be classified as Brucella melitensis as DNA hybridization studies have shown that the genus contains only one species.
Brucella species are aerobic, capnophilic and catalase-positive. Apart from B.ovis and B.neotomae, they are oxidase-positive. All Brucella species are urease-positive except B.ovis. Brucella ovis and some biotypes of B.abortus require 5 to 10% CO2 for primary isolation. Moreover, the growth of other Brucella species is enhanced in an atmosphere of CO2. Media enriched with blood or serum is required for culturing B.abortus biotype 2 and B.ovis. Recently, brucellae have been detected in sea-mammals. Smears are made from specimens and stained by the modified Ziehl- Neelsen (MZN) stain. Brucellae appear as small, red-staining coccobacilli in clumps because of their intracellular growth.
Isolation
The brucellae grow well on 5-10 per cent blood agar. However, other than foetal abomasal contents and colostrum, the specimens are likely to contain many contaminating bacteria and fungi, so selective media are usually required. The selective media contain a nutritive blood agar base with 5 per cent sterile seronegative equine or bovine serum and an antibiotic supplement. The antibiotic
supplement used in selective media for B.ovis usually differs from that for B. abortus. Skirrow agar is a satisfactorymedium for both the Campylobacter fetus subspecies and for brucellae, including the most fastidious species such as B. abortus biotype 2, B.canis and B.ovis.

Brucellosis, one of the most ancient diseases, continues to be a problem for humans and animals throughout the world causing enormous economic losses and significant human morbidity in endemic areas. The bacterial pathogen is classified by the CDC as a category B pathogen that has the potential for development as a bioweapon. It is one of the important zoonotic disease and considered as the most common laboratory acquired pathogen with >5 lakh human cases reported annually around the world. Brucellosis is a chronic infection which can result in infertility, delayed heat, interrupted lactation, loss of calves, wool, meat and milk production in animals and undulant fever in humans. Although in most of the counties, brucellosis is a nationally notifiable disease and reportable to the local health authority, it is underreported and official numbers constitute only a fraction of true incidence of the disease. Although the true incidence of human brucellosis is unknown, the estimated burden of the disease varies widely from <0.03 to >160 per 1 lakh population. Although only 17 countries in the world are free from the disease, it has been reported in many countries including India. Bovine brucellosis is endemic in all the states in India and appears to be on the increase in recent time, perhaps due to increased trade and rapid movement of livestock. The preponderance of natural bull service in rural India is perhaps yet another important factor in the maintenance and spread of infection. The Government of India has made it mandatory to regularly screen all the breeding bulls from artificial insemination centres, for brucellosis and to use brucellosis free bulls for semen production.

Etiology: Brucella sp. are facultative intracellular Gram - ve coccobacilli, nonspore forming and non-capsulated. It infects macrophages with principal involvement of liver, spleen and bone marrow. Nine Brucella species are currently recognized, seven of them that affect terrestrial animals are: B. abortus, B. melitensis, B. suis, B. ovis, B. canis, B. neotomae and B. microti and two that affect marine mammals are B. ceti and B. pinnipedialis. The first three species are called classical Brucella and within these species seven biovars are recognized for B. abortus, three for B. melitensis and five for B. suis. The remaining species have not been differentiated into biovars.

Synonyms : Bang’s disease, Contagious abortion, Infectious abortion, Enzootic abortion, Undulant fever, Malta fever, Mediterranean fever

Brucellosis, one of the most ancient diseases,  continues to be a problem for humans and animals throughout the world causing enormous economic losses and significant human morbidity in endemic areas. The bacterial pathogen is classified by the CDC as a category B pathogen that has the potential for development as a bioweapon. It is one of the important zoonotic disease and considered  as the most common laboratory acquired pathogen with >5 lakh human cases reported  annually  around the world. Brucellosis is a chronic infection which can result in infertility, delayed heat, interrupted lactation, loss of calves, wool, meat and milk production in animals and undulant fever in humans.

Brussels sprouts are thought to have originated in the Belgian city of Brussels, from which they got their name. Records in the Brussels area date back to the 13th century. In the 18th century, the French coined the term. They are not native to the United States and have never been seen growing wild. Brussels sprouts are a type of cabbage (Brassica oleracea var. gemmifera) that belongs to the mustard family Brassicaceae and are commonly produced in Europe and North America for its edible buds known as sprouts. It is cultivated for its tasty buds. The leaf veggies have a diameter of 1.5-4.0 cm (0.6-1.6 in) and resemble small cabbages.

Botany & taxonomy Brussels sprouts are a type of Cole vegetable that belongs to the Brassicaceae family.

The word BRYONIA means growing rapidly, as the stem grows rapidly.

ALBA, from Latin word ALBUS means white as the roots and flowers are yellowish or white.

Bryonia affects especially the constitution of a robust, firm fiber and dark complexion, with tendency to leanness and irritability. It prefers the right side, the evening, and open air. Warm weather after cold days, to manifest its action most markedly. Bryonia acts on all serous membranes and the viscera they contain.

Bryonia gives remarkable relief in muscular pain, which aggravates on motion, in the treatment of bronchitis and inflammatory and congestive headache.

The character of the pain is "Stitching and tearing". An interesting feature of Bryonia pain is that patient feels better, ameliorated if pressure is applied to the painful part. The patient is comfortable when lying on the painful side.

1. Introduction
 
The bryophytes are found everywhere in the world except in the sea. They grow on wet soil or rock, the trunk of trees, in lake, river and even in Antarctic island. The bryophytes are placed taxonomically between algae (Fig. 1) and pteridophytes (Fig. 2); there are about 24,000 species in the world. They are further divided into three phyla, Bryophyta (mosses 14,000 species, Fig. 3), Marchantiophyta (liverworts 6,000 species, Fig.4a,b) and Anthocerotophyta (hornworts 300 species, Fig. 5). They are considered to be the oldest terrestrial plants, although no strong scientific evidence for this has appeared in the literature. This hypothesis was mainly based on the resemblance of the present-day liverworts to the first land plant fossils, the spores of which date back to almost 500 million years. Among the bryophytes almost all liverworts possess beautiful cellular oil bodies (Fig. 6) which are peculiar, membrane-bound cell organelles which consist of ethereal terpenoids and aromatic oils suspended in carbohydrates- or protein-rich matrix, while the other two phyla do not. These oil bodies are very important biological markers for the taxonomy in the Marchantiophyta (Asakawa, 1982, 1995; Asakawa et al., 1979b, 2008c, 2012b, 2013a,b; Asakawa and Ludwiczuk, 2008a,b; Ludwiczuk and Asakawa, 2010).

Cotton is a major crop of India grown in about 9.0 million hectares.This is the largest cotton area in the world. Thus, India ranks Ist among cotton growing countries in the world in terms of area. However, average cotton yields in India have been 320 kg/ha as compared to world cotton yield average of 580 kg/ha. Thus, cotton production in India represents only about 13% of the total world production whereas India accounts for approximately 20% of the world’s total cotton area. Obviously, yield levels in India are low compared to world average. The low yield levels in India are partly due to heavy losses from insect pests, particularly cotton bollworm complex.

During 2019, the global acreage of biotech/GM crops was 190.4 million ha and India planted 11.9 million ha GM cotton having insect resistance (Bt-cotton, having two commercialized events, namely, cry1Ac, MON-531, MahycoMonsanto, popularly called by trade name Bollgard-I, single gene event, commercialized in 2002 and cry2Ab2, MON-15985, Mahyco-Monsanto, popularly called by trade name Bollgard-II, double gene event or stacked trait event, commercialized in 2006) stood at the fifth place after USA, Brazil, Argentina and Canada (ISAAA, 2019). Four more events related to Bt cotton (cry1Ac, Event-1, JK Agri-Genetics, commercialized in 2006, fused genes cry1Ab and cry1Ac, GFM Event, Nath Seeds, commercialized in 2006, cry1Ac BNLA-601, UAS, Dharwad and CICR (ICAR), commercialized in 2008 but discontinued and synthetic cry1C, MLS-9124, Metahelix Life Sciences-now Tata Rallis, commercialized in 2009 but not placed in the market) were approved for commercialization but the products could never reach to the market.

Buckwheat (Fagopyrum esculentum Möench) is an annual pseudocereal crop belonging to the genus Fagopyrum of the family Polygonaceae. Its grains belong to cereals because of their similar use and chemical composition (Campbell, 1997). Among a number of buckwheat species nine species possed agricultural and nutritional trials common buckwheat (F. esculentum) and tartary buckwheat (F. tartaricum) are frequently cultivated around the world (Krkošková and Mrázová, 2005).

1.1 Introduction In parallel with the expansion of organically cultivated land and low-input agricultural system, there is an increasing demand for crops that have promising potentials to be grown successfully in these systems. Buckwheat is generally sown in smaller areas, which may be found suitable for low-input agricultural systems. Buckwheat is a fast-growing crop with a variety of utilizations. Buckwheat, one of the pseudo and minor-cereals, has the ability to satisfy the rapidly rising population’s ever-increasing food need in the nation’s hill regions in the context of changing climatic conditions. It is also known as common buckwheat or Fagopyrum esculentum, it is not a cereal grain, but rather a seed that is related to rhubarb and sorrel. It has a unique nutty flavour that makes it an excellent ingredient in a variety of dishes. It is known in India by a variety of names, including ogal, phaphar, bresha, mittahe dyat, dro, and brotitae.

Budgeting is a fundamental component of financial modelling programs and data analytics, serving as a structured approach to planning, allocating, and controlling financial resources within an organization. It involves estimating future revenues and expenses based on historical data, current trends, and strategic objectives. When combined with data analytics, budgeting becomes more dynamic, accurate, and responsive, enabling organizations to make informed financial decisions and adapt to changing conditions. The budgeting process begins with data collection and preparation. Historical financial data, including past revenues, operating costs, and capital expenditures, is gathered from various sources such as accounting systems and databases. Data analytics tools help clean, organize, and validate this information, ensuring consistency and reliability. This step is crucia

Introduction
 
Livestock sector plays an important role in Indian economy and is an important sub sector of India agriculture the contribution of livestock to gross domestic product was 4.7% in 2004-05 with 27% share in agriculture and allied sector. Milk production in India reaching a level of 91 million tones in 2004-05. It is estimated that in milk production 58% of milk was contributed by buffaloes with average lactation milk about 1300 kg. Some of the buffaloes produce as high as 4000 kg but their number is limited. India with 185 million cattle and approximately 98 million buffaloes possesses 20% of the world bovine and 14% of the cattle population. Buffalo population in the country has been increasing at a steady wait with slight increase in productivity. Highest increase in the past five years was observed in Haryana (25.1% ), followed by Uttar Pradesh (20.6%), Madhaya Pradesh (13.9%), Gujrat (13.6%), Uttaranchal (12.2%), Andhra Pradesh (10%) and Rajasthan (6.6%). Productivity per animal is very low mainly due to low genetic potential for milk production, near absence of the genetic improvement programmes, poor plan of nutrition and health measures. 

Dairy buffalo production has been a tradition in parts of the world like the Caucasian countries. Asia and Egypt, where fresh buffalo milk, dahi (cultured sour milk), ghee (butter oil) and yoghurt are popular. In Italy the dairy buffalo industry is flourishing thanks to the popularity of buffalo mozzarella cheese. Because of the market for Mozzarella, buffalo farming is a profitable enterprise and is carried out in an organized manner with modern equipment. In South American countries like Brazil and Argentina, buffalo reared for both milk and meat. In recent years buffalo milk and milk products, especially Mozzarella cheese, have become immensely popular and dairy buffalo production has founds its way into non traditional areas with the number of buffalo farms mushrooming.

The buffalo belonged to the Bovidae family, Bovinae subfamily and the scientific name is Bubalus bubalis. This is descended from the Bubalus arnee or wild Indian buffalo, and is widely dispersed throughout Southern Asia. The world population of buffaloes is approximately 204 million head: more than 98% are in Asia; 0.8%are in Africa, particularly in Egypt; 0.9% are in South America; and 0.2% are in Europe (FAO, 2024).

The countries with the largest numbers of dairy buffaloes are India, Pakistan, China, Nepal and Egypt. In Pakistan and Nepal there are more dairy buffaloes than dairy cows. Water buffaloes are the main source of milk in South Asia. India is the largest water buffalo milk producer compared to Pakistan, where buffaloes produce more milk than cattle.

 Scientific classification
 Genus: Bubalus 
 Species: bubalis
 Wild water buffalo: Bubalus arnee
 Domestic water buffalo: Bubalus bubalis
 Lowland anoa: Bubalus depressicornis
 Mountain anoa: Bubalus quarlesi
 Tamaraw: Bubalus mindorensis
 Cebu tamaraw: Bubalus cebuensis

India is home to an enormous number of buffalo. According to the 20th Livestock Census, more than 109 million buffaloes, or more than 56% of the world’s population, are living in India. With 20 recognized breeds, it also has a very diverse collection of buffalo genetics. One of the most well-known buffalo breeds in the world, the Murrah, is also found in India. The buffalo is a versatile animal that can be used for milk, meat, and draught. India is the world leader in this industry, producing 76% of the world’s buffalo milk.

Abstract

Buffalo is the second major source of milk supply in the world. India, Pakistan, China, Egypt, Nepal, Myanmar, Italy, Sri Lanka, Iran, and Turkey are the top ten countries of highest buffalo milk production. In Asian countries, Riverine buffalo is mostly used milk purpose breed. Buffalo milk is a plentiful source of nutrients including fat, protein, essential fatty acids, essential amino acids, lactose and beneficial oligosaccharides, indispensable fat and water soluble vitamins, vital minerals. It contains more total solids, fat, protein, lactose and calcium as compared to cow milk. Buffalo milk possesses wide potential to be used in the production of a range of dairy products. On account of the differences in physico-chemical and biochemical properties between buffalo and cow milk, conventional processing technology and equipment for cow milk may not be suitable for buffalo milk processing. Changes and modifications must be considered for making certain products. This chapter aims to highlight the nutritional profile including amino acid profile, lipid profile and mineral composition; physico-chemical properties and potential of buffalo milk in the dairy sector.

Buffalo farming offers a sustainable and economically viable solution to meet the growing demand for safe food production, particularly in regions with rising food insecurity. Buffaloes contribute around 45% of India’s total milk production and are more efficient in converting low-quality feed into high quality milk and meat, making them ideal for marginal farming environments. Buffalo milk, which has higher fat, protein, and mineral content than cow milk, is in demand for value-added dairy products, such as cheese, and fetches higher market prices. This enhances farmers’ incomes, especially small-scale dairy farmers, by ensuring higher returns on investment. Additionally, buffalo husbandry mitigates risks in crop failure due to climate change and provides sustainable employment opportunities. Furthermore, buffaloes produce less greenhouse gas emissions per unit of meat compared to cattle, making them more environmentally sustainable and climate smart. By advancing breeding practices and adopting scientific management, buffalo farming has the potential to drive the future of safe and sustainable food production, contributing significantly to food security and rural development.

A buffer is an aqueous solution consisting of a mixture of a weak acid and its conjugate base or a weak base and its conjugate acid. Its pH changes very little when a small amount of strong acid or base is added to it and thus it is used to prevent changes in the pH of a solution. Buffer solutions are used as a means of keeping pH at a nearly constant value in a wide variety of chemical applications. Many life forms thrive only in a relatively small pH range so they utilize a buffer solution to maintain a constant pH. One example of a buffer solution found in nature is blood.