eChapters

ABSTRACT

In the studies on callus induction and somatic embryogenesis in acid lime (Citrus aurantifolia S.) var Sai-Sharbati, different ex-plants and different combinations of growth regulators (NAA, IBA and BA) in MS basal media were tried, the results indicated that cotyledonary segment was the best explant for callus induction among root tip hypocotyl stem, leaf, epicotyl stem and shoot tip explants tested of. The callus grown from epicotyl and hypocotyl stem explant was greenish white and compact. Whereas callus derived from cotylidonary segment and leaf segment was green and friable . Root tip and shoot tip did not exhibit callusing. The callus derived from epicotyl stem and hypocptyl stem explant responded shoot bud formation in MS medium supplemented with NAA 0.2mg/l + BA 0.5mg/l with or without ME 100mg/l. In somatic embryogenesis, full grown callus derived from epicotyl stem explant induced embryo formation within 28.33 days and more shoot bud (6.33) formation.

Modes of Culture 

Callus is a mass of undifferentiated plant tissues formed from plant cells or tissue cuttings when grown in culture. Tissues and cells of plant cultured in a liquid medium aerated by agitation grow as suspension of single cells and cell clumps. For growth, the cells require to divide, whereas, the cells breaking up from explant are mature, nondividing. Hence, the differentiated tissue undergoes modifications to become meristematic. This phenomenon of a mature cell reverting back to meristematic state to form undifferentiated callus tissue is called dedifferentiation.

Camel Nomenclature Family: Camelidae Order: artiodactyla (even –toed ungulates) Suborder: Tylopoda (Pad on foot) The family Camelidae includes two genera: Camelus and Lama The genus Camelus consists of two species namely. Camelus dromedarius (unicorn or dromedary) with habitat in dry, hot, arid areas of Africa, Asia and Australia and Camelus bactrianus Double-humped or Bactrian) habitat in cold, dry areas of the central Palearctic Ecozone. These two species are commonly called Old World camels. The Species belonging to the genus Lama are Lama vicugna  Vicunaand Lama guanaco guanaco, Lama glama (llamaand Lama pacos alpaca . These four Species are called New World camels and have their habitat in South America Camels are considered a “ship of the desert” as a means of transport and Towing ability in the desert. The camel also played an important role in civil law and order, defense and battles from antiquity to the presen

Camels could be exploited for augmenting the country’s milk production capacity as a supplementary milk resource in India for the benefit of increasing human population. Since milk production potential of camels in India have remained unexploited and considering its therapeutic utility it can fetch good prices. Camels are raised for milk, meat, fibre (wool and hair), transport and other work; their dung is used as fuel. Milk is often the most important camel product and is the staple food of nomads.

The camel is an important species of the Indian fragile desert ecosystem, a proven icon of adaptation with its unique bio-physiological characteristics and has formidable ways of living in harsh situations of arid and semi-arid regions. The proverbial Ship of Desert earned its epithet on account of its indispensability as a mode of transportation and draught ability in desert. However, its utilities are subject to continuous social and economic changes. The camel has also played a significant role in civil law and order, defence and battles from the ancient times till date. Camels formed an important component of the Mauryan Army (C.322-232 BC) and continued through the Mughal period (1200-1700 AD) to the present times.

Abstract

Camel milk has been known in Asia and Africa for 5000years for its benefits for human health. It is the closest to human mother’s milk and safe for children. Research studies around the world have confirmed that camel milk has better nutritional value as it exhibits many unique and amazing health-promoting properties as compared to cow milk. This milk is considered as a complete food and can be consumed exclusively while meeting all nutritional requirements. Many studies have reported that camel milk contains very high concentrations of mono- and polyunsaturated fatty acids, low fats, cholesterol, and lactose. It also contains higher amounts of minerals (calcium, iron, magnesium, copper, zinc, and potassium), vitamins B2 and C as compared to cow milk. It does not contains beta lactoglobulin and beta casein that are present in cow milk and that are the main cause of allergy in certain human population. Furthermore, camel milk contains various protective proteins that exert antibacterial, antiviral, and immunological properties. It possesses unique, powerful immune system–boosting components that would exert potential health benefits. It is an alternative for people suffering from cow milk allergy. So camel milk can be considered as next generation super food.

Origin and Distribution of Camel

A camel is an even-toed ungulate within the genus Camelus of the family Camelidae bearing distinctive fatty deposits known as “humps” on its back. There are two species of camel, viz. Camelus dromedarius (single humped dromedary) having habitat in dry hot arid lands of Africa and Asia, and Camelus bacterianus (double humped or bactrian) habitat of cold arid lands of Central palaerctic (a zoogeographical region consisting of Europe, Africa north of the Sahara, and most of Asia north of the Himalayas) region. Family Camelidae also includes other species which are camel like mammals. Those are Lama (Lama glama), Alpaca (Lama pacos), Vicuna (Vicugna vicugna) and Guanaco (Lama guanicoe). These four species are termed as new-world Camelidae having habitat in South America. The Indian camel belongs to Camelus dromedarius. A very small numbers of double-humped camels are also available in Ladakh area of Jammu and Kashmir.

Camel is an important component of desert eco-system. It is capable of traveling long distance in hot, arid and sandy regions carrying men, material and providing energy to agricultural operations. They provide milk, meat, hair and used as work animals. It is believed that Camels were domesticated during the period from 3000 to 2500 BC. Average life expectancy of a camel is 40-50 years. The height at the shoulder and hump of a fully grown adult camel is 1.85 and 2.15 metres, respectively. Camel can run at a speed of up to 65km/h for a short distance and can sustain a speed of up to 40km/h for long distance.

Camels across the world are reared for milk, meat, draught, race and tourism. They are distributed in the African, Arabian and south Asian countries.  Apart from this, a feral population exists in Australia and recent reports indicate that a few animals of the species are also being reared in Europe. The world camel population shows an increasing trend with 12.92 m in 1961 to  24.68 m in 2010 producing 0.38 m tonnes of meat and 2.36 m tonnes of milk per annum.  The top 20 camel rearing countries produce both milk and meat with the exception of India and Pakistan as per FAO. Contrary to this, the research articles and news reports indicate that the species in the two south Asian countries too produce both milk and meat.

The Camellia is one of our most beautiful and most appreciated ornamental plants belonging to the family Theaceae. The genus Camellia was named by Linnaeus after the Jesuit botanist “Georg Joseph Kamel”. Camellias represent gratitude, love and perfection and are a perfect way to tell someone they possess those qualities. The camellia is the state flower of Alabama. Industrial use of Camellia is in the oil industry. Seeds of several species belonging to the sections Oleifera, Paracamellia, Camellia and Furfuracea are used for extracting edible oil, which is used extensively for cooking in China (Ming, 2000, Gao et al., 2005 and Zhang et al., 2007). In China, more than 3 million hectares are used for camellia oil production and nearly 645000 tons of seeds are harvested each year, which yield nearly 164000 tons of edible oil (Ming, 2000 and Gao et al., 2005).

ORIGIN AND DISTRIBUTION

Since it is a native of warm temperate regions (southern China) and thrive throughout a wide area extending from Washinton, D.C., south through Georgia and Florida, west along the Gulf Coast and north along the Pacific Coast as far as Seattle. Species of Camellia are largely distributed in south-eastern and eastern Asia and China, which, being the centre of species diversity, possess more than 80% of the species (Gao et al., 2005).

Diploid Camellia reticulata was discovered in the Jinshajiang valley, Yunnan and Sichuan provinces, China, at an elevation of 1650-2800 m and under high humidity conditions (Xia et al., 1994). Yunnan province is the centre of origin for C. reticulata (Xia et al., 1994). The most important ornamental species is C. japonica L., of which there are over 32000 registered cultivars (Savige, 1993). Today, plants from C. japonica, C. reticulata, C. sasanqua and a group of yellow-flowering species called golden camellias are grown as ornamental plants worldwide. The family can be divided into three groups of similar genera (Prince and Parks, 2002).

The  Camellia  is  one  of  our  most  beautiful  and most  appreciated ornamental  plants belonging to the family Theaceae. The genus Camellia was named by Linnaeus after the Jesuit botanist “Georg Joseph Kamel”. Camellias represent gratitude, love and perfection and are a perfect way to tell someone they possess those qualities. The camellia is the state flower of Alabama. Industrial use of Camellia is in the oil industry. Seeds of several species belonging to the sections Oleifera, Paracamellia, Camellia and Furfuracea are used for extracting edible oil, which is used extensively for cooking in China (Ming, 2000, Gao et al., 2005 and Zhang et al., 2007). In China, more than 3 million hectares are used for camellia oil production and nearly 645000 tons of seeds are harvested each year, which yield nearly 164000 tons of edible oil (Ming, 2000 and Gao et al., 2005).

Camera Technology Overview

A camera is a device used to capture and store images and videos, either digitally through an electronic image sensor or chemically using a light sensitive material like photographic film. This technology has been crucial in the fields of photography and videography, significantly impacting visual arts, media, entertainment, surveillance, and scientific research. Since its invention in the 19th century, the camera has undergone numerous technological advancements, resulting in a diverse range of types and models in the 21st century.

Etiology

There are 18 species in genus Campylobacter. Campylobacter species in poultry include C. jejuni, C. coli and C. lari. The most predominant species is C. jejuni.

Epizootiology

The infection has been reported in chicken, but the organisms have been isolated from ducks, turkeys, black birds, sparrow and pigeons.

Transmission

Possibly through faeces, as organisms are in the intestinal tract. Egg transmission of bacteria on or in the egg through hatchery is strongly suggested.

In 1886, Escherich observed Campylobacter like organisms in stool samples of children with diarrhoea. In 1913, McFaydean and Stockman first confirmed them as Campylobacter in foetal tissues of aborted sheep. Confirmatory tests were also carried out by Smith in 1918 when similar organisms were isolated from aborted bovine foetuses. The organism was then known as Vibrio foetus. In 1963, due to certain differentiating characteristics, the organisms were separated from Vibrionaceae family and the new genus Campylobacter (means ‘curved rod’) under Campylobacteriaceae family was proposed. 

Properties of genus

1.   Morphology :  Campylobacter is gram negative, comma shaped rods specially in infected tissues and young cultures. The size is 1.5-4 µm by 0.5 µm. When two bacterial cells are found together in a microscopic field, it looks like ‘S’ or ‘wing of gull’ (‘flying seagull’). However in older cultures, the chains of organism appear as long spirals. They are motile by single unipolar/ bipolar unsheathed flagella. Motility is darting or corkscrew type, best observed by dark field microscopy. They are non-sporing. Some species contains S-layer (surface layer), a paracrystalline protein structure composed of S-layer proteins (SLPs). It is external to the outer membrane. It helps the bacteria in immune evasion.

Campylobacter species are slender, curved motile Gram negative rods (0.2 to 0.5 pm wide) with polar flagella. Daughter cells which remain joined have a characteristic gull-winged appearance and long spirals formed by joined cells also occur. These microaerophilic organisms grow best on enriched media in an atmosphere of increased CO2 and decreased oxygen tension. Many Campylobacter species grow on MacConkey agar. They are non-fermentative and oxidase-positive and have variable catalase reactions.
Usual Habitat
Many Campylobacter species are commensals in the intestinal tracts of warm-blooded animals. Campylobacter jejuni and C. lari (formerly C. laridis) colonize the intestines of birds, which can result in faecal contamination of water courses and stored food. A number of Campylobacter species are excreted in the faeces of pigs. Campylobacter fetus subspecies venerealis appears to be adapted principally to bovine preputial mucosa.
Differentiation of Campylobacter Species
Campylobacter species are strictly microaerophilic, requiring an atmosphere of 5 to 10% oxygen and 1 to 10% CO2 for growth. A selective enriched medium such as Skirrow agar is usually used for primary isolation. Differentiation of isolates is based on colonial morphology and certain cultural, biochemical and antibiotic-susceptibility characteristics.
Colonial Morphology
• Campylobacter fetus subspecies venerealis and C.fetus subspecies fetus have small, round, smooth, translucent colonies with a dewdrop appearance.
• Campylobacter jejuni produces small, flat, grey colonies with a spreading, watery appearance.

Introduction

In the past, when television sets entered almost every household and there was a set of TV channels that broadcasted news, daily soaps, songs, etc., a channel named "Discovery" entered the market and soon became very popular among all age groups due to the scientific documentaries that it broadcasted and soon enough took over the market. It's obvious to the eyes that they created something unique, innovative, and possessing utility. The success behind the TV channel was that it tapped the uncontested market and hence had no other channel of its kind to compete with. They created a new niche, and based on this, they generated huge profits, success, and popularity. This strategy is very famous today in the name of the “Blue Ocean Strategy” introduced by Kim and Mauborgne (2005). The same strategy has immense potential in alleviating the agripreneurial ventures wherein the greater homogeneity of the products brings in an issue of increased competition. Hence, being creative and innovative on the part of entrepreneurs can open greater avenues for new products in the market, wherein the new product may benefit from the uncontesting environment in the market.

Abstract

The functioning and stability of terrestrial ecosystem are determined by plant biodiversity and species composition. So far, little attention has been paid to the effects of microbe-plant interactions, particularly the mycorrhizal symbiosis, on ecosystem variability, productivity and plant biodiversity. The most common type of mycorrhizal association is the arbuscular mycorrhizal (AM) type. The role of AM fungi in the uptake of diffusion limited nutrients like P and in conferring resistance to soil-borne-diseases is well documented.

Technical Term                    Definition

Canal Irrigation System      The system in which irrigation water is supplied through irrigation canals network (main canal, submain canal, distributaries, and

                                            minors) from source of water (reservoirs) to point of application (agricultural lands)

Summary

RNA interference has revolutionized the concepts in regulation of gene expression. RNA interference is the mechanism that controls sequence specific gene silencing by double stranded RNA. These non-coding RNAs are gene specific regulators critical in the transformation of normal cells to a malignant stage. Alterations in the non-coding RNAs have been linked to specific cancers and can serve as diagnostic markers. The ability of exogenously introduced siRNAs to specifically bind its complementary mRNA and down regulate its expression has various applications. It has markedly influenced the way the role of certain genes is investigated in tumorigenesis. The siRNA technology has been effectively functional in identification of new components and blocking the expression of critical mediators in tumorigenesis.

Abstract

C. albicans is a diploid, dimorphic yeast producing three morphologic forms: yeast cells, pseudohyphae and true hyphae. It is an opportunistic pathogen causing various types of candidiasis which are on the increase around the globe. It is a polymorphic yeast with its genome organized in eight diploid chromosome with its genome size ranging between 14.3 and 16 Mb having 5,733 to 6, 318 genes. It has two mating-type like (MTL) alleles that control the mating behavior. In its genetic makeup it resembles with Saccharomyces cerevisiae hence classified in the Saccharomycetales. The chromosome number from tetraploid to diploid is brought about by the parasexual mechanism. Keeping in view the increase of incidence of candidiasis due to C. albicans around the globe, it is important to identify the causative organisms to the species level correctly. Morphological, biochemical, germ tube test and growth on different media are often used for identifying isolated yeasts which sometimes takes days to week to develop in culture hence, rapid identification of Candida species in clinical laboratory is becoming increasingly important. Molecular techniques utilizing amplification of target DNA or PCR amplification of conserved regions of the genome and sequencing of the resulting PCR product and restriction fragment length polymorphism (RFLP) analysis of the ribosomal DNA, provides quick and precise methods for diagnostics or, identification of Candida species.

Introduction

1. Serious outbreaks of thrush have been observed in chickens, pigeons, geese, turkeys, pheasants, quail, peacock and many other species of birds.
2. Candida albicans is the most commonly isolated fungus from the outbreak. Mucor spp. and Aspergilli were also found in some cases.
3. The outbreaks are associated with unhygienic conditions and nutritional deficiencies. Infection is more common in young birds up to 10 weeks of age. Morbidity is very high and mortality ranges from 10-75%. Clinical signs are not specific. Poor growth and ruffled feathers are only clinical signs.

Canine mammary gland tumours are the most common type of tumour in the intact female dog. Of all species, dog develops neoplasm twice as frequently as humans, with incidence of skin and mammary tumours being the highest. Tumours of mammary gland are the most common tumours of female dog representing approximately 30-50% of all tumours in bitch and it is the second most common tumour in dog after skin neoplasm and most common malignant tumour in dog.

Defination

Mammary gland tumours arise primarily in the female animal from secretary epithelial, myoepithelial, or, less frequently, mesenchymal elements of mammary tissue.