eChapters

7.1. Rural Poverty in Nigeria

The Federal Republic of Nigeria has a population of more than 160 million - the largest in Africa - and a fast-growing economy. Agriculture is the mainstay of the economy, contributing about 40 per cent of GDP. The agriculture sector employs approximately two-thirds of the country’s total labour force and provides a livelihood for about 90 per cent of the rural population. Nigeria is the world’s largest producer of cassava, yam and cowpea - all staple foods in sub-Saharan Africa. It is also a major producer of fish. Yet it is a food-deficit nation and imports large amounts of grain, livestock products and fish. Nigeria’s huge agricultural resource base offers great potential for growth. Recent government policies have started to show results: The agricultural sector reportedly grew by 7 per cent a year between 2003 and 2007, and at a slightly lower rate in recent years. Still, the area of land under cultivation could be doubled. Of an estimated 71 million hectares of arable land, only about half is presently under production. And there is substantial scope for an increase in irrigation, which now covers only 7 per cent of irrigable land. Irrigation and other inputs would substantially increase average yields for major staple crops, which are below those in other developing countries.

Despite Nigeria’s plentiful agricultural resources and oil wealth, poverty is widespread in the country and has increased since the late 1990s. Some 70 per cent of Nigerians live on less than US$1.25 a day. Poverty is especially severe in rural areas, where up to 80 per cent of the population lives below the poverty line, and social services and infrastructure are limited. The country’s poor rural women and men depend on agriculture for food and income. About 90 per cent of Nigeria’s food is produced by small-scale farmers who cultivate small plots of land and depend on rainfall rather than irrigation systems. The poorest groups eke out a subsistence living but often go short of food, particularly during the pre-harvest period. The productivity of the rural population is also hindered by ill health, particularly HIV/AIDS, tuberculosis and malaria.

Climate change is caused by both natural as well as anthropogenic causes and it has been changing since the inception of earth-atmosphere (Figure 2.1). In a broader sense, anthropogenic activities (human activities) also must be considered as natural one in longer time scale as human and their activities are also a part of natural-ecosystem-functions. Among the natural phenomena, volcanic eruptions and continental drifts significantly affect the earth’s precipitation and temperature. A huge volume of CO₂ is released due to volcanic eruption at a little span of time-period which heated up the earth-atmosphere. Climate variability is primarily governed by rise in sea temperature, movement of warm water from the Western Pacific towards Peru, and El Nino which occurs at an intervals of 3-7 years. Climate variability also influenced the alteration in temperature and precipitation world wide. And persistent climate variability cause long term climate change. Therefore, basically three drivers that causes climate change are, the alteration in the energy of sun received by earth-atmosphere (natural causes), variations of ref lectivity of earth-surface- atmosphere system (natural as well as anthropogenic causes) and fluctuations in the greenhouse effect (natural as well as anthropogenic causes).

Plant diseases are classified on the basis of type of pathogenic or nonpathogenic causes of the disease. The classification is based on the plant pathogenic organisms as follows.
1. Parasites
They include both biotic and mesobiotic agents. The diseases are incited by parasites under a set of suitable environment. Association of definite pathogen is essential with each disease.
i. Biotic agents: They are also called as animate causes. They are living organisms.Biotic agents include
1. Prokaryotes
a. True bacteria or bacteria (Facultative parasites) e.g. Citrus canker.
b. Rickettsia-like bacteria (RLB) e.g. Citrus greening, Pierce’s disease of grape.
c. Mollicutes or wall-less prokaryotes
i. Mycoplasma-like organism (MLO) e.g. Sesame phyllody, Egg plant little leaf.
ii. Spiroplasma e.g. Corn stunt, Citrus stubborn.

Introduction
Plant diseases significantly impact agricultural productivity and food security, causing substantial economic losses worldwide. The causes of these diseases can be broadly categorized into inanimate and animate factors, each contributing uniquely to plant health. Understanding these causes is essential for developing effective management strategies to prevent and mitigate disease outbreaks.
A. Inanimate Causes
a) Environmental Factors: Environmental conditions play a crucial role in the development and severity of plant diseases. Key environmental factors include:
• Temperature: Each plant species has an optimal temperature range for growth. Deviations from this range can stress plants, making them more susceptible to pathogens. For example, high temperatures can accelerate the life cycle of some fungal pathogens, while low temperatures can inhibit plant growth, creating conditions conducive to disease.
• Moisture: Water is essential for plant growth, but too much of it can become a problem. High humidity levels and frequent rainfall create an ideal environment for many fungal diseases. Pathogens like Phytophthora and Fusarium thrive under such wet conditions, spreading rapidly in overly moist soils or plant canopies. On the flip side, drought can also lead to trouble. When plants are under water stress, their natural defenses weaken, making them easier targets for both disease-causing organisms (biotic stress) and harmful environmental factors (abiotic stress).

Manifests its action mainly in chronic rheumatic, arthritic and paralytic affections, indicated by the tearing, drawing pains in the muscular and fibrous tissues, with deformities about the joints; progressive loss of muscular strength, tendinous contractures. Broken down senile. In catarrhal affections of the air passages, seems to choose preferably dark - complexioned and rigid fibered persons. Restlessness at night, with tearing pains in joints and bones, and faint - like sinking of strength. This weakness progresses until we have gradually appearing paralysis. Local paralysis, vocal cords, muscles of deglutition, of tongue, eyelids, face, bladder and extremities. Children are slow to walk. The skin of Causticum person is of a dirty white sallow, with warts, especially on the face. Emaciations due to disease, worry etc. Burning, rawness and soreness are characteristic.

Causticum is indicated in the treatment of chronic rheumatism and paralysis accompanying it. Here, Causticum ranks with Rhus Tox and Sulphur.

Key words : CAVD, Drug Design, Bioinformatics, CADD, Co-factor, Vaccinology, infectious diseases, Jenner, immunity, Molecular modeling, Immunoinformatics, Antigen, Antibody, Live Vaccine, Alive Vaccine, Protein-peptide, Enzyme, Tools for Vaccine design,
 
Vaccine – Prevention is Better than Cure

A vaccine is a biological preparation that improves immunity to a particular disease. A vaccine typically contains an agent that resembles a disease-causing microorganism, and is often made from weakened or killed forms of the microbe, its toxins or one of its surface proteins. The agent stimulates the body’s immune system to recognize the agent

1. Introduction
Animal experimentation remains a cornerstone of scientific research in many fields, from medical advances to pharmaceutical testing. However, the ethical treatment of laboratory animals has become a point of significant concern worldwide. India, through the Committee for the Control and Supervision of Experiments on Animals (CCSEA), ensures that ethical standards are maintained in research institutions. Established under the Ministry of Environment, Forests, and Climate Change (MoEF&CC), CCSEA is tasked with overseeing the use of animals in experiments, ensuring that procedures adhere to ethical guidelines and international norms. CCSEA’s guidelines are in line with international standards set forth by organizations such as the International Council for Laboratory Animal Science (ICLAS) and the European Union (EU), but they are tailored to address local needs and conditions. The guidelines, which focus on the welfare of laboratory animals, emphasize the ethical review process, animal care, and the importance of minimizing animal suffering. They also stress the need for researchers to implement the 3Rs—Replacement, Reduction, and Refinement—to minimize the use of animals and ensure humane practices in research. This chapter explores the CCSEA guidelines in detail and compares them with animal welfare regulations from around the world, including the United States, European Union, Japan, and Australia. By understanding the similarities and differences between these regulations, researchers and policymakers can ensure the ethical treatment of laboratory animals on a global scale.
2. Objectives of CCSEA Guidelines
The main objective of the CCSEA guidelines is to ensure that laboratory animals are treated ethically and humanely while also supporting the validity and scientific rigor of research. The guidelines encourage researchers to prioritize animal welfare while minimizing unnecessary suffering. Specifically, CCSEA focuses on:
1. Ethical Treatment of Animals: Ensuring that animals are housed in suitable conditions, given adequate veterinary care, and provided with a comfortable environment to reduce stress.
2. Justification of Animal Use: Ensuring that animals are used only when necessary, and that their use is scientifically justified.
3. Pain and Stress Minimization: Ensuring that experimental procedures cause the least pain, distress and suffering possible.

1.1 Policy Background

Human activities have resulted in the alteration of the composition of our atmosphere triggering change in the Earth’s climate. The world’s population has grown at an alarming rate with a corresponding increase in demand for natural resources, energy, food, and goods. As a consequence of increase in consumption, vast quantities of gases and effluents are discharged that change the composition of the atmosphere and its capacity to regulate its temperature. The rise in the global temperature is caused by the accumulation of the so-called “greenhouse gases”, namely, carbon dioxide (CO₂), methane, nitrous oxide, and chlorofluorocarbons. Energy received from the sun is absorbed as short wavelength radiation and is eventually returned to space as long wavelength infrared radiation. Greenhouse gases absorb the infrared radiation, trapping it in the atmosphere in the form of heat energy.

Objective: To synthesize cDNA from isolated plant total RNA.

Background information:

Complementary DNA (cDNA) is double-stranded DNA made from template of single stranded RNA, [e.g., mRNA or microRNA] by reverse transcriptase enzyme. Mostly cDNA is used for cloning of eukaryotic genes in the prokaryotes. For the expression of a specific protein in a cell where the expression of that protein is not found (i.e., heterologous expression), the cDNA will be transferred that codes for the protein to the recipient cells. Retroviruses (e.g. HIV-1, HIV-2, simian immunodeficiency virus, etc.) also produce cDNA naturally and integrate in host's genome and further creates provirus (Croy, 1998). Major use of cDNA is in gene cloning or as gene probes or in the creation of a cDNA library. When scientists transfer a gene from one cell into another cell for the expression of new genetic material as a protein in the recipient cell, the cDNA will be added to the recipient (rather than the entire gene), because the DNA for an entire gene may have certain DNA that may not code for the protein or that interrupts the coding sequences of the protein (e.g., introns). Partial sequences of cDNAs are often obtained as expressed sequence tags.

Dr. Chauhan is an exceptional individual who had achieved a significant milestone in his academic and professional journey. Completing a Bachelor of Science (Honours) with an impressive 80.3%, Dr. Chauhan’s accomplishment is a reflection of his hard work, dedication, and unyielding passion for the field of veterinary science. This remarkable achievement not only underscores his
academic brilliance but also cements his position as an inspiring figure in the veterinary community.
From an early age, Dr. Chauhan demonstrated an innate love for animals and a deep sense of responsibility toward their well-being. These qualities formed the foundation of his journey in veterinary science. His BVSc&AH (Honours) degree, completed with such distinction, is not just a testament to his intellectual capabilities but also to his tireless commitment to improving animal health and welfare. Pursuing a degree in Veterinary science is no small feat. It demands a unique combination of scientific expertise, practical skills, and emotional resilience. For Dr. Chauhan, the rigorous academic journey involved mastering a wide array of subjects, including animal Anatomy, Physiology, Pathology, Pharmacology, and Microbiology. Each subject posed its own set of challenges, yet he tackled them with unwavering determination and an insatiable thirst for knowledge. His exemplary performance, as evidenced by his impressive score of 80.3%, highlights his ability to excel even under pressure. What sets Dr. Chauhan apart is not just his academic excellence but also his holistic approach to learning. Beyond textbooks and lectures, he actively sought opportunities to gain hands-on experience, volunteering at animal shelters and participating in community outreach programs. These experiences not only enriched his understanding of animal care but also shaped his perspective as a compassionate and empathetic veterinary professional.

Introduction

Seasonality in agriculture is a major determinant of poverty, food insecurity and hunger in areas having harvest only once or twice a year. Undiversified livelihoods survive on the crops that the households consume as food and sell for income. The risk being high, a single failed harvest can cause huge losses and can destitute a marginalized household with limited or no savings, increasing their dependence on borrowings leading to indebtedness later on. Punjab being one of the pioneer states in green revolution in country is facing lot of challenges due to monoculture i.e. paddy-wheat as the major crop rotation followed on 92% area of the state. The situation has led to serious repercussions in agriculture sector of the state along with social, financial, and ecological issues. 

Introduction 
It is a common vegetable crop of rabi season which belongs to the family Apiaceae (Umbelliferae). Due to prevalence of protandry condition, the crop is cross pollinated in nature and self-pollination within individual flower is restricted. It comprised of three distinct cultivated varieties grown over the world for various economic purposes and classified as below depending upon usefulness of different parts.
Origin
It found is globally distributed in New Zealand, Australia, South Africa, Mediterranean basin, and South America, whereas Argentina and Chile are the richest sources. Habitat is extensively ranging from Egypt, Algeria, and Abyssinia to Sweden.

Celery was grown as an aromatic and therapeutic herb by Egyptians, Greeks, and Romans. It was first grown in Italy in the 16th century after this wild variety. It was then introduced to the British Islands by the Italians and French at the end of the 17th century. It was first grown in North America in the nineteenth century. Celery’s young leaves and stem are high in minerals, vitamins, and proteins, making it one of the most essential therapeutic plants. Celery oil has a pleasant aroma. This aromatic oil aids in the efficient functioning of the human brain. On an area of roughly 5000 acres, the crop is grown primarily in Punjab (Jallandhar, Gurdaspur, and Amritsar districts), Haryana, and western Uttar Pradesh (Ladhwa and Saharanpur districts). Punjab provides almost 90% of the overall production

English name : Celery
Botanical name : Apium graveolens L.
Family : Apiaceae
Chromosome number : 2n = 22
Centre of origin : Italy
Celery is botanically known as Apium graveolens L. and is commercially recognized by different names in India, like Karnauli, Ajmod, or garden celery. There are four horticultural varieties of celery. Apium graveolens var. dulce (Miller) Pers. is cultivated for its edible leaves and stalks, commonly used in salads, appetizers, and soups. Another variety, var. rapaceum (Mill) Gaudich, known as celeriac, comprises dark green leaves grown for its root tubers, which are cooked and consumed. The other two varieties are var. secalinum and smallage.
In India, the main variety cultivated is var. dulce. Celery is commercially available in various forms, including seeds, flakes, as a vegetable, celery seed oil, and oleoresin. Celery seeds have specific quality characteristics viz. moisture content ranges from 5-11%, volatile oil content from 1.5-3% (average 2.4%), non-volatile ether extract from 5.8-14.2% (average 9.4%), cold water extract from 5.9-12.6% (average 8.4%), total ash from 6.9-11.0% (average 8.8%), and ash insoluble in acid from 0.5-4.0% (average 2.5%).

The cell number of a particular cell type in a total cell population might indicate the physiological or pathological status of the cell smear/FNAC/tissue. ImageJ is a powerful open- source software extensively used for image assessment, including cell counting. In this chapter, the steps to count cells using ImageJ has been discussed. There are two ways to count the cells in a research image using ImageJ.
A. Manual Counting
Use the “Cell Counter” plugin or simply use the multipoint tool in ImageJ. Open it, select the cell type you want to count, and click on each cell in the image to tally them. Double-click on the multipoint tool. A separate window will open named point tool. One can select the type of marking, color and size of making (Figure 30). Keep clicking on the cell type to be counted. It will record the number of cells counted. If one wants to count the second cell type in the same image, change the counter number and select the size, color and type of click point.

A finite growth capacity is a characteristic of all cells derived form the normal animal tissue. The cells pass through a series of age related changes before being incapable to divide further. The finite number of generations of growth is a characteristic of the cell type, age and species of origin and referred to as the “Hayflick limit”. The cells derived from embryonic tissue have a greater growth capacity than those derived from adult tissue.

Introduction

Cell culture is a technique that involves the isolation and in vitro maintenance of cells isolated from tissues or whole organs derived from animals, microbes or plants. This technique has immeasurable role in the field of biology. The scientific progress in biology, microbiology and biotechnology in recent years can be credited on the development of cell culture technology. With the development of cell culture the isolation of viruses has become less cumbersome, the assessment of the efficacy and toxicity of new drugs has been easier, new vaccines are being developed and there has been development in biotechnology assisted reproductive technology.

In general, animal cells have more complex nutritional requirements and usually need more stringent conditions for growth and maintenance. For the growth of the cell in vitro specific mediums are required by the cells. Previously natural mediums like plasma ,serums were used but with the advent of synthetic media the specific needs of different cell types can be catered with. Cell culture is governed by a provided set of protocols requiring a sterile pure culture of cells, various nutrients for its growth, sterile cell culture hood, appropriate aseptic techniques and the utilisation of suitable conditions for optimal viable growth of cells.

Plant cell culture
Introduction

As the human population is expanding there has been increasing concern over whether current rates of food productions can support the growing population. In response to the increasing demand, scientists and farmers have been working hard to come up with new ideas to meet these needs. These ideas range from simple procedures including selection of the best seeds to more complex procedures requiring the transplantation of genes into different plant species. This chapter focuses on one of the important aspects of plant science which is the growth of plant cells in an artificial media. This process is known as plant cell culture and can be applied in many different ways.