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Soil is the largest terrestrial ecosystem where a wide variety of relationships exists between different types of soil organisms. The associations existing between different soil microorganisms, whether of a symbiotic or antagonistic nature, influence the activities of microorganisms in the soil. Microflora composition of any habitat is governed by the biological equilibrium created by the associations and interactions of all individuals found in the community. In soil, many microorganisms live in close proximity and interact among them-selves in a different ways. Some of the interactions or associations are mutually beneficial, or mutually detrimental or neutral.

Industrial microbiology is an important area of applied microbiology. In a broader sense, industrial microbiology is concerned with all aspects of business that relate to microbiology. It refers to the use of microorganisms in commercial enterprise. Cheap raw materials are converted to valuable products through the metabolism of microbes. Microbes for this purpose could be exploited in different ways.

Various commercial products of economic value made by microbes are :

Silage is preserved pasture. Fresh forage crops such as maize, grasses, legumes, wheat and lucerne can be preserved by ensiling. Silage is the green material produced by controlled fermentation of the green fodder crop retaining the high moisture content, which contain 20-40% dry matter.

Silage can be prepared from plants having thick stems and are generally not very suitable for hay making like sorghum, maize. Weeds can also be utilized along with main fodder crops for silage making. Silage making kills majority of weed seeds. It is highly palatable. The organic acids produced in the silage are similar to those normally produced in the digestive tract of the ruminants and therefore are used in the same manner.

Introduction

Biofertilizers are microbial preparations containing living or latent cells of efficient strains of nitrogen fixing, phosphate solubilizing and cellulose decomposing microorganisms intended for seed or soil application and designed to improve soil fertility and plant growth by increasing the number and biological activity of beneficial microorganisms in the soil.

The objects behind the application of biofertilizers /microbial inoculants to seed, soil is to increase the number and biological / metabolic activity of useful microorganisms that accelerate certain microbial processes to augment the extent of availability of nutrients in the available forms which can be easily assimilated by plants. The need for the use of biofertilizers has arisen primarily due to two reasons i.e. though chemical fertilizers increase soil fertility, crop productivity and production, but increased / intensive use of chemical fertilizers has caused serious concern of soil texture, soil fertility and other environmental problems.Use of biofertilizers is both economical as well as environment friendly. Therefore, an integrated approach of applying both chemical fertilizers and biofertilizers is the best way of integrated nutrient supply in agriculture.

Biofertilizers, thus include i) Symbiotic nitrogen fixers- Rhizobium sp. ii) Non-symbiotic, free living nitrogen fixers- Azotobacter, Azospirillum etc. iii) Blue Green Algae (BGA) inoculants- Azolla-Anabaena, iv) Phosphate solubilizing microorganisms (PSM)- Bacillus, Pseudomonas, Penicillium, Aspergillus etc. v) Mycorrhiza vi) Cellulolytic microorganisms

Mycorrhizal symbiosis is the most prevalent and important mutualistic association in plant kingdom. ‘Mycorrhiza’- the word means fungus - root, this association was reported by A.B.Frank in the year 1885. Nearly 95% of plant species form this mycorrhizal association. This serves as critical linkage between plant roots and soil and characterized by the movement of plant produced carbon to fungus and fungal acquired nutrients to plants. Through its hyphae this fungus absorbs the available P from soil and transports directly to root cortex. Mycorrhizal associations vary widely in structure and function. There are seven types of mycorrhizae (Table 1). These are endo (arbuscular), ecto, ectendo, arbutoid, monotropoid, ericoid, and orchidaceous mycorrhizae. Among them, endomycorrhizae and ectomycorrhizae are the most abundant and widespread

For many soil borne diseases, effective and economic control methods are not available. Several soil borne pathogens have developed resistance to many fungicides. The beneficial microbes are also affected by fungicides. Many fungicides pose health hazards by polluting food and water and cause direct toxicity of heavy metals and carcinogenic risk. In the context of growing worldwide awareness of environmental pollution and development of fungicide resistant strains of pathogens, biological method of suppression of disease causing organisms have been recognized as the safest approach for plant disease management.

Biological control of plant diseases is the suppression of populations of plant pathogens by living organisms. Amongst beneficial microorganisms, isolates can be selected which are highly effective against pathogens and can be multiplied on artificial media. Application of such selected and mass produced antagonists in high densities once or several times during a growing season is called “augmentative biological control”.

Chemical pesticides though extremely effective in controlling pest population suffer from disadvantages. They have a broad toxic range and potentially toxic to non target organisms. Other constraints that have emerged with the use of chemical pesticides such as development of resistance and resurgence in insects, residual toxicity, secondary outbreak of minor insect pests, etc., In this juncture, the biopesticides have proven to be effective tool in managing the devastating insect pests of various crops.

The biopesticides are types of pesticides derived from natural materials such as microorganisms, plants, animals and certain minerals. They are living organisms or their products or byproducts, which can be used for the management of pests that are injurious to crop plants. Biopesticides have a vital role in crop protection, although most commonly in combination with other tools including chemical pesticides as part of integrated pest management (IPM). Microorganisms help to regulate the abundance of many pests or potential pests without any cost to society. Microbes can be increased artificially and thereby achieve faster, higher and more consistent control of the target pests that will occur naturally. Insect pathogens that are transmissible in nature from one host to another offer an important bonus effect that chemical insecticides do not have. A pathogen will kill pests exposed and many continue killing them by natural transmission from one individual host to another through subsequent host generation.

Biopesticides generally have several advantages compared to conventional pesticides. While chemical pesticides are responsible for extensive pollution of the environment, a serious health hazard due to the presence of their residues in food and development of resistance in targeted insect pest populations, biopesticides, in contrast, are inherently less toxic to humans and the environment, do not leave harmful residues, and are usually more specific to target pests. The agents employed as biopesticides, include parasites, predetors and disease causing fungi, bacteria and viruses, which are the natural enemies of pests. Utilization of naturally occurring parasites, predators and pathogens for pest control is a classical biological control. These bio agents can be conserved, preserved and multiplied under laboratory condition for field release. Once these bio-agents are introduced in the field to build their population considerably, they are capable of bringing down the targeted pest population below economic threshold level (ETL).

Mass Production of Bacterial Inoculants

Strain Selection and Improvement

In biofertilizer technology, strain selection occupies the foremost criteria, so as to explore its biological potentialities for the beneficiary crop. The selected strain used in biofertilizer production must be efficient to grow fast besides retain desirable potentialities of biological activities, which should be present in desired high numbers and must be stable. The strain must be improved periodically so as to sustain the productivity.

Biofertilizers are one of the important components of organic farming and sustainable agriculture. Awareness among farmers is increasing on the biological fertilizers. But, the success of biofertilizer inoculation depends on the quality of the inoculant used. Because farmers are unable to judge the quality of the inoculants at the time of purchase or even at use. The inoculant producers are negligent to institute quality control programmes. Unfortunately much of the inoculant produced in the world today is relatively of poor quality. In such situation maintaining high quality inoculant products depend on the internal implementation of effective quality control systems by manufacturer. A major goal of a quality standard is to control the quality of inoculant to be used in any country.

Bacterial biofertilizers are supplied as carrier based inoculants. Peat or lignite is used as carrier material. Carrier based bacterial inoculants are applied by the following methods.

1. Seed treatment or seed inoculation.
2. Seedling root dip and
3. Main field application

Seed Treatment

One packet of the inoculant (200 g) 7is mixed with 200 ml of rice kanji to make a slurry. The seeds required for an acre are mixed in the slurry so as to have a uniform coating of the inoculant over the seeds and then shade dried for 30 minutes. The shade dried seeds should be sown within 24 hours. One packet of the inoculant (200 g) is sufficient to treat 10 kg of seeds.

At this moment, the majority of energy is obtained from fossil fuels and requires switching over to alternative energy sources is the need of the hour. Fossil fuels are nonrenewable energy source and also have seriously negative impacts on the environment. The use of fossil fuels cause excessive global climate change because emissions of greenhouse pollutants and the formation of compounds CO_x, NO_x, SO_x, C_xH_y, ash and other organic compounds that are released into the atmosphere. The increase in greenhouse gas emission will result in global warming, climate change, environmental degradation and health problems.

Biofuel, any fuel that is derived from biomass - that is, plant or algae material or animal waste. Since such feedstock material can be replenished readily, biofuel is considered to be a source of renewable energy, unlike fossil fuels such as petroleum, coal, and natural gas.

Ber (Ziziphus maruritiana Lam.) is cultivated all over the desert part of the Indian subcontinent for its fresh fruits, which are rich in vitamin C, vitamin A and vitamin B-complex and minerals. It can be successfully cultivated even in the most marginal ecosystems of the subtropics and tropics. Ber or Indian jujube belongs to the genus Zizphus of the family Rhamnaceae and order Rhamnales. Ber is adopted to ecologically poor, drought prone areas, which encounter recurrent crop failures and thereby economic crises. It yields nutritious fruit, rich in vitamins and minerals than apple even in drought years and its cultivation is highly cost effective owing to very low input requirement. Ber cultivation also provides employment for people in different farm operations for production and sale of the produce. In India, Haryana, Punjab, Uttar Pradesh, Rajasthan, Gujarat, Madhya Pradesh, Bihar, Maharashtra, Andhra Pradesh and Tamil Nadu are major ber producing states. The area under cultivation with this fruit is 8.7 lakh ha with an annual production of 8.9 lakh tones in India (Baloda et al., 2012). About 125 varieties of ber are available in India. A few of these varieties are known for taste, size, amount of pulp and higher yields. The cultivars Umran, Kathapal and Gola are the most promising varieties of ber in North India (Azam-Ali et al., 2001).

Nutritive Value

Many people are unaware that the ber is one of the most nutritive fruit, since ber is a common fruit. It is one of the richest sources of vitamin C, next only to aonla and Guava but better than citrus fruit and apple. The fruit pulp contains 13-20% total soluble solids and 0.20-0.80% acidity at fully ripened fruit. Ripe fruit of Umran contains 117 mg ascorbic acid/100 g of pulp. Ber fruit contains β-carotene (vitamin-A) at 81 µg/100 g of fruit, 5.6% sucrose, 1.54% glucose, 2.08% fructose, 1.00% starch and 1.13% total carbohydrates. Due this, so ber is often called the “poor man’s fruit”. Table 1 represent the nutritional composition of different ber varieties.

Cape gooseberry is the native of Peru and Chili (South America), but widely cultivated in Cape of Good Hope and hence called so. It is known by different name in various parts of the world. It is commonly called ‘Poha’ in Hawaii; ‘Golden berry’,’ Pompelmoes’ or ‘Apelliefie’ in South Africa; ‘Lobolobophan’ in the Philippines. In India it is commonly called ‘Rasbhari’, ‘Makoi’, ‘Tiparee’ or ‘Tepari’. Cape goose berry (Physalise peruviana L.) belongs to the family solanaceae. Out of 80 species of genus physalis, only three are well known for their fruit value. These are: (i) P. pubescen L. (ii) P. Peruviana L., and (iii) P. ixocarpa Brot. However these three species, Physalise peruviana L is the most popular due to its pleasant flavour and acid-sweet taste.

Chicory (Cichorium intybus L. var. sativum D.C.) plant is the herbaceous, perennial plant and supposed to have descended from the wild chicory (wild succory, blue sailors) which is often seen along roadsides with its blue flowers. The leaves are used as salads in human diet and other different purposes. They were reported to be stomachic (Baumann 1982, Franke, 1981) and their juice was used against eye disease and poisoning. The infusion of chicory was believed to be good for the liver, kidney and stomach. Initially the wild plant was used but since approximately 300 B.C., the crop was cultivated for this purpose. Beverages, soups and sauces made of chicory were reported to be used in Italy and Belgium as early in 16^th century.

For the first time, the root of chicory was used as a substitute of coffee in 1690 in Holland followed by many other countries like Sweden, France, etc. During 1806 and 1813, the cultivation and use of roasted roots of chicory as coffee substitute spread over the whole European continent. In the 19^th century, the main centres of cultivation of chicory were North-East France, Belgium, England, Russia, Holland and Australia. Subsequently it was raised on large scale in Poland, Hungary, Germany, Switzerland and India. Presently over 20% of the World’s consumption takes place in Eastern Europe, especially in Poland (6%) followed by Russia, Hungary, Romania, Czechoslovakia and East Germany. Twelve percent is reported to be consumed in South Africa, 9% in India and 2% in the United States (Clark and Macrae, 1987).

Ginger is used extensively as a spice in cuisines throughout the world. Ginger belongs to Zingiberaceae family and an English botanist William Roscoe (1753-1831) gave this plant the name Zingiber officinale in the year 1807. The name of the genus Zingiber derives from a Sanskrit word denoting “horn shaped” in reference to the protusions on the rhizomes (Ghosh et al., 2011). It is an oldest rhizome widely domesticated as a spice where the edible part is the swollen underground stem or rhizome. The Zingiberaceous plants have strong aromatic and medicinal properties and are characterized by their tuberous or non-tuberous rhizomes (Chen et al., 2008). Ginger is a medicinal plant that has been widely used all over the world, since antiquity, for a wide array of unrelated ailments including arthritis, cramps, rheumatism, sprains, sore throats, muscular aches, pains, constipation, vomiting, hypertension, indigestion, dementia, fever and infectious diseases (Ali et al., 2008). Ginger has direct anti-microbial activity and thus can be used in treatment of bacterial infections (Tan and Vanitha, 2004).

Mushrooms are good as nutritious food for all ages and under all conditions of health. The mushroom is a fleshy, space-bearing organ of non-green edible fungi. Its fleshy nature of mushroom is responsible for its main attraction to human being as a source of food. Like other fruit and vegetables, mushrooms are soft textured and highly perishable in nature. Mushroom are potential sources of nutrients. They can convert nutritionally valueless substances into high fat and protein food. Mushroom proteins are comparable to muscle protein in nutritive value. Being a good source of vitamins and proteins it is considered be a distinct food. The digestibility of protein in these is 72-83%. Mushrooms are edible fungi; assume considerable importance in human diet as they are good source of non starchy carbohydrate, dietary fibre, minerals and vitamins-B and essential amino acids. Mushrooms are popular their delicacy and flavour rather that food value. However, it is now a well-established fact that they are excellent sources of vitamins and minerals (Vijaya Khader, 1988). The vitamins in mushroom are well retained during cooking, canning, and dehydration. The White button mushrooms (Agaricus bisporus), Shiitake mushroom (Lentinus edodes), Pleurotus species (Pleurotus osteatus, P. flabellatus, P. sajor caju etc.), Paddy straw mushrooms (Volvariella volvacea), Winter mushrooms (Flammulina velutipis), Jew’s ear mushrooms (Auricularia polytricha) are commercially cultivated species of mushroom. In India, mainly three species viz., white button, oyster or dhingari and paddy straw mushrooms are commercially cultivated at large scale.

Tropical fruit tree are important crops which supplement and improve the quality of diet. Many of the species have multi-purpose uses in that they produce non food products such as fuel, timber, fodder, medicines and industrial products for small holders. Harvesting from these trees enable rural people to provide nutrition for a balanced diet and generate income thus alleviate poverty. Tamarind (Tamarindus indica L) can be found throughout much of the tropics, it is grown unattended in backyards, roadsides or waste lands. Tamarind is ideally suited for avenue plantings as a roadsides tree, in and around villages as a multipurpose species for agro-forestry system or as protective firebreaks for forest margins. It is a valuable timber species widely used for making furniture, tool handles, charcoal, oil mills, rice pounders and fuel wood. The leaves are an important source of food and herbal medicine and the edible pulp of ripe fruits is used as a flavouring agent in cooking, soups, jams, chutneys, sauces, pickles etc. The fruit pulp of tamarind forms the “Tamarind of Commerce”. The name tamarind is derived from the Arabic word ‘Tamar-u’l-Hind’ because the dark brown pulp of the fruit was thought to resemble dried dates. It was therefore called the Tamere-hindi or ‘date of India. The production of Tamarind in India was estimated 201000 tonees for year 2017-18 (Horticulture statistics, 2018), India is the world’s largest producer of tamarind products. It is particularly abundant in the India states of Madhya Pradesh, Bihar, Andhra Pradesh, Karnataka, Tamil Nadu and West Bengal. The production in India is mainly concentrated in the drier southern states and the produce is collected by the villagers and sold in the market. In India, the average production of tamarind pods per tree is 175 kg and processed pulp is 70 kg/tree (Kulkarni et al., 1993).

A large number of people in developing countries have traditionally depends on products derived from plants, especially from forests, for curing human and livestock ailments. Additionally, several aromatic plants are popular for domestic and commercial uses. Collectively they are called medicinal and aromatic plants (MAPs). About 12.5% of the 4,22,000 plant species documented worldwide are reported to have medicinal values. With dwindling supplies from natural sources and increasing global demand, the medicinal and aromatic plants, will need to be cultivated to ensure their regular supply for processing and value added products.

The term “Medicinal and Aromatic plants” generally denotes those non-aromatic and aromatic plants, which are used for medicinal, perfumery, dye and cosmetic purposes. They are rich in secondary metabolites and are potential sources of drugs. Generally the whole plant roots, stem, bark, leaves, flowers, fruits, gums and oleoresins, etc. are used.

India has been considered as treasure house of valuable medicinal and aromatic plant species. Ministry of Environment and Forests have identified and documented over 9500 plant species considering their importance in the pharmaceutical industry. In the present context of ‘back to nature’ in health care, it is relevant that these valuable plant species are not only preserved but also their cultivation developed in order to meet the entire demand of the domestic industries as also to exploit the bright prospect for export. Shift from collection to cultivation of medicinal & aromatic plants will also ensure purity, authenticity and sustainable supply of raw materials required for herbal drugs, including polyherbals.

India has a population of one hundred twenty four crore plus and feeding this large a population calls for a strong FPI. The industry also assumes importance in the national economy due to the immense employment generation opportunities it offers and the export revenue it earns. Food Processing Industry (FPI) in India is a sunrise sector that has gained prominence in the recent years. Easy availability of raw materials, changing lifestyles and favourable fiscal policies has given a considerable push to the industry’s growth. FPI serves as a vital link between the agriculture and manufacturing sectors of the economy. Strengthening this link is critical to reduce wastage of agricultural raw materials, improve the value of agricultural produce by increasing shelf-life as well as by fortifying the nutritive value of the food products and ensure remunerative prices to farmers as well as affordable prices to consumers. The industry has very low processing level i.e., 2.2% for fruits and vegetables, around 35% in milk, 21% in meat and 6% in poultry products, which is significantly lower by international standards. For e.g., processing of agriculture produce is around 40% in China, 30% in Thailand, 70% in Brazil, 78% in the Philippines and 80% in Malaysia. Value addition to agriculture produce in India is just 20% with wastage estimated to be valued at around US $13 billion.

India has the second largest producer of food with arable land of 159.65 million hectares and has the highest acreage under irrigation. Next to China, India ranks second largest food producer in the world and has the potential to immerge the biggest with its food and agricultural sector. India accounts for less than 1.5% of international food trade despite being one of the world’s major food producers, which indicates huge potential for both investors and exporters. Food and food products are the biggest consumption category in India

The major components of foods are the protein, carbohydrates and fat. The spoilage of foods containing fats includes the deterioration of the fatty components; the principal difficulties found in their preservation are in the development of rancidity. The spoilage of fats and oils has received much attention and substantial progress has been made in their preservation. One of the major causes of food deterioration is the chemical oxidation of unsaturated fatty acids in the lipids present in all foods. These fats are present in fat deposits and in cell membranes. End products of lipids oxidation, mainly aldehydes and ketones, contribute to the rancid off flavour and odours in the foods.

Fatty acids in food occur in the form of esters with the alcohol. The predominant esters are triacylglycerols accompanied by minor amounts of mono- and diacylglycerols and free fatty acids. The unsaturated bonds present in all fats and oils represent active sites that can react with oxygen. This reaction leads to formation of primary, secondary, and tertiary oxidation products that may make the fat or fat containing food unsuitable for consumption. Other consequences of oxidation include discolouration; changes in the texture, flavour and taste; and loss of nutritional value due to the destruction of essential fatty acids as well vitamins. Unsaturated fatty acids are subject to oxidation at their double bonds. In the presence of suitable catalysts, or in contact with lipooxidase enzymes, long chained unsaturated fatty acids may be fragmented to short chained fatty acids. The cleavage usually involves an intermediate peroxide formation. Many chemicals compounds aid in food preservation to protect the nutrients, flavour and texture, and storage stability of food stuff for longer period.

A white to light brown powder, is a heteropolysaccharide derived from the cell wall of higher terrestrial plants. It is mainly used in food as a gelling agent in jams and jellies. Today it is also used in fillings, sweets, as a stabiliser in fruit juices and milk drinks and as a source of dietary fiber in foods. Pectin is a heterogeneous grouping of acidic structural polysaccharides, found in fruit and vegetables and mainly prepared from ‘waste’ citrus peel and apple pomace. Pectin has a complex structure. Preparations consist of sub-structural entities that depend on their source and extraction methodology. Commercial extraction causes extensive degradation of the neutral sugar-containing side chains.

Pectin was first isolated and described in 1825 by Henri Braconnot, though the action of pectin to make jams and marmalades was known long before. To obtain well set jams from fruits that had little or only poor quality pectin, pectin-rich fruits or their extracts were mixed into the recipe. During industrialization, the makers of fruit preserves soon turned to producers of apple juice to obtain dried apple pomace that was then cooked to extract pectin. Later, in the 1920s and 1930s, factories were built that commercially extracted pectin from dried apple-pomace and later citrus-peel in regions that produced apple juice in both the USA and in Europe. At first pectin was sold as a liquid extract, but nowadays pectin is often used as dried powder that is easier to store and handle than a liquid

Fruit and vegetables play an important role in human nutrition. They are vital source of essential minerals, vitamins and dietary fibre and supply complex carbohydrate and protein. They are good source of calcium, phosphorus, iron and magnesium and contribute over 90% of dietary vitamin C. Green and yellow fruits and vegetables are a rich source of vitamin A (β-carotene). Thiamine, niacin and folic acid, which are required for normal functioning of the human body, are also present in significant qualities. Fruit is an important food in diet designed to reduce the risk of coronary heart disease in developed countries. It is not surprising, then, that certain fruit components exert pharmacological or therapeutic effect. The antioxidant properties of β -carotene may also a role in prevention of some forms of cancers. Packing and handling systems have been developed to move the product from farm to consumer expeditiously in order to minimize quality degradation. Food irradiation is a physical process like drying, freezing, thermal processing (canning and pasteurization) that can be used to disinfest, sterile and preserve food. The use of chemical preservatives to inhibit the growth of spoilage bacteria in modern food system has been recognized as deterrent to health. The present day, customer demands food products with no chemical preservatives, free from additives, natural with assured safety and better long shelf life.

The deterioration in foods during storage may include loses in organoleptic desirability, nutritional value, safety and aesthetic appeal. Food is subjected to physical, chemical and biological deterioration. Heat, cold, light, humidity, other radiation, gas composition, moisture, dryness, natural food enzymes, micro and macro organism, and industrial contaminants are adversely affect the food quality and their shelf life. The practice of drying, curing and fermentation are among the conventional methods of preservation used for thousand years.

The paddy grain contains a rough outer covering called husk. It accounts for about one-fifth to one - fourth of the weight of paddy. The inner kernel, called brown rice or dehusked rice, again contains some soft outer layers, jointly called bran. It accounts for about 6-8% of the brown rice weight, including the small germ (1-2% by weight) located in one corner. Rice milling is the process of removing the husk and a part of the bran from paddy in order to produce edible rice. Rice milling system ranges from the home-scale to the large, complex modern rice-processing installations. They include hand-pounding equipment, single hullers, emery sheller-cum-huller mills, emery sheller-cum-cone polisher mills and the modern rubber – roller rice mills. Rice milling industry does not only mill rice but it also carries many other essential functions, such as cleaning and grading, drying, storage, quality control etc. for the production of better quality rice. All these processes are discussed in this paper.

Unlike other food grains, rice is mostly consumed as cooked whole grain, Milling technology is therefore geared to obtain maximum outturn of milled rice and to reduce breakage to the minimum. The single huller mills are by and large located in villages or in localities where paddy is custom milled for produces and they still handle the bulk of the country’s production. The large-capacity rice mills located in urban or semi-urban areas for commercial milling are of ½ to 4 tones per hour capacity and handle the rest of the paddy milled. Some battery hullers are still in operation as large commercial mills, specially for milling parboiled paddy.

Foods are classified into three groups on the basis of ease of spoilage. Spoilage meant a condition produced by the excessive growth of microorganisms leading gradually to decay or decomposition. The stable or non perishable foods like sugar, flour, grains and dry products do not ordinarily spoil unless handled carelessly. The semi-perishable food like potatoes, onions, apples, waxed fruits, fruits and vegetables having thick skin can be protected from spoilage if properly handled and processed. The perishable foods, like livestock products, seafood, and poultry items, dairy products, and most fruit and vegetables spoil readily unless special preservatives methods are used. The spoilage of foods may be caused by molds, yeast, bacteria, enzymes, food constituents and insects. The use of food additives have become necessary to prevent spoilage other wise caused by the growth of bacteria, yeasts and molds.

Food spoilage usually refers to undesirable changes occurring in food due to the action of micro-organisms, insects and enzymes. The original nutritional value, texture, flavour of the food are damaged, the food become harmful to people and unsuitable to eat. Today however, preservation has used such factors as temperature, water activity, pH, gases, organic acids, salts, antibiotics, irradiation, packaging and various combinations of these factors. Foods vary greatly in the length of time for which they can be held in their natural form without spoilage. Preservatives retard product spoilage caused by mold, air, bacteria, fungi or yeast. Bacterial contamination can cause food-borne illness, including life-threatening botulism.

In India, people are mostly vegetarian, depending largely on cereals and pulses, as their staple foods, which provide the main source of dietary proteins and calories. The proteins from pulses are known to be inferior quality, due to the deficiency of sulphur containing amino acids as well as due to other factors like digestibility, availability of amino acids, anti-nutritional factors, etc. The anti-nutritional factors present in this type of food, including their contents of enzyme inhibitors, lectins, flatulence factors, tannins, phytic acid and saponins. Anti-nutritional factors can cause detrimental effects to humans and animal growth and performance by impairing intake, uptake, or utilization of other food and feed components, or by causing discomfort and stress to humans and animals. The anti-nutritional factors mainly occur in pulses and grain legumes and foods and feed material prepared from grain legumes and pulses.

Substance which, from nutritional point of view, interferes with normal growth, reproduction or health when consumed regularly in amount existing in a normal component of diet should be considered as harmful and toxic. A significant part of human population relies on legumes as staple food for subsistence, particularly in combination with cereals. They are unique foods because their rich nutrient content including starch, protein, dietary fibre, oligosaccharides, phytochemicals (especially the isoflavones in soybean) and minerals. Their nutritional contents contribute to many health benefits to human.

Food technologies now have more sweeteners from which to choose than ever before. With the obesity epidemic and the public attention it currently receives, plus the increasing interest in foods with added benefits sweeteners that allow for consumer appealing labeling are of particular interest. These sweeteners fall into basic categories, those that are essentially calories free, often referred to as low-calories or intense sweeteners, and those, which are significantly reduced in calories, which may be referred to as reduced calories-sweeteners, bulk sweeteners, or sugar replaces. These sweeteners when used alone or in combination may permit such labeling as ‘low-calories’, ‘reduced-calories’, ‘light’ ‘sugar-free’ and ‘dose not promote tooth decay’.

The ideal sweeteners does not exist, it would be at least as sweet as sucrose and provide the same properties to a product as sucrose, with processing parameters similar to those of sucrose so that existing equipment can be used. It would be colourless, odourless and noncariogenic, with a clean, pleasant taste and have immediate onset and not much lingering. Solubility and stability are important. The ideal sweetener must be compatible with a wide range of food ingredients because sweetness is but one element of a complex food flavour system. Even sucrose is not perfect, being unsuitable for some applications.

Extrusion cooking is an emerging technology having advantages of low production cost, capable of producing end products in various shapes and sizes, from a variety of food materials with easy digestibility, maintaining hygiene and high product quality and leaving no effluents or hazardous materials. Minimal energy consumption and processing costs and possibility of flavour addition are the added advantages of technology. The process/technique has the versality and ability to constituently produce a given product in continuity at a given set of pre – determined specifications. It destroys fat splitting enzymes to avoid rancidity and thus extends the shelf life of products considerably. Microorganisms present in food materials are destroyed. It can be effectively utilized to develop functional and designer foods by using several locally available under-utilized food materials. However, it leads to increased package cost as puffed or expanded food products have low bulk density. R & D studies are further needed for co – extrusion of variety of cereals with high fibre content foods and micronutrients.

Fruit jelly is an important part of diet of all human being. It is easily digestible, highly refreshing, appetizing and nutritionally far superior to many foods. Today, a wide variety of fruit jellies are consumed in the home, at work place and sport stadium. There has been considerable increase in the consumption of fruit and vegetable jellies in the world during last few years. Jelly is a semi-solid product prepared by boiling a clear strained fruit extract free from pulp after the addition of required amount of sugar, citric acid and pectin. It should contain minimum 65 percent of total soluble solids and minimum 45 percent of fruit portion (Dhawan, 1998). Guava is rich source of pectin and acid content and have thick flesh is preferred for jelly making. For getting good quality jelly, mix under-ripe fruits give better pectin content and ripe fruits provide the flavor. Winter season fruits yield better quality jelly (Singh and Dhawan, 1983). It is a clear or translucent fruit spread made from sweetened fruit (or vegetable) juice and set using naturally occurring pectin. Additional pectin may be added where the original fruit does not supply enough. Jelly can be made from sweet, savory or hot ingredients. It is made by a process similar to that used for making jam, with the additional step of filtering out the fruit pulp after the initial heating.

Jellies are the products used as bread spreads all over the world. These are made from fruits rich in pectin. Guava, because of its rich pectin content, is processed into jelly. Processing of guava into jelly is practiced by processors all over the world. Colouring of jams and jellies has been an age old practices colorants. Great attention is being paid now days to the usage of natural colours in view of the hazards that the synthetic colours pose to human health. Incorporation of carrot resulted in colour improvement over control. Jelly containing carrot as a colouring source was found slightly better than other vegetables. Jelly is a semi-solid product prepared by boiling a clear strained fruit extract free from pulp after the addition of required amount of sugar, citric acid and pectin. It should contain minimum 65% of total soluble solids and minimum 45% of fruit portion (Dhawan, 1998).

Much of the post-harvest losses of fruits and vegetables in developing countries is due to the lack of proper storage facilities. While refrigerated cool stores are the best method of preserving fruits and vegetables they are expensive to buy and run. Consequently, in developing countries there is an interest in simple low-cost alternatives, many of which depend on evaporative cooling which is simple and does not require any external power supply. India is the second largest producer of fruits and vegetables in the world after Brazil and China respectively. Storage of fresh horticultural produce after harvest is one of the most pressing problems of a tropical country like India. India’s total fruits and vegetables production is lost during harvest, storage, grading, transport, packaging and distribution in a year which reduces the growers share. Due to their highly perishable nature, about 20-40% of total fruit and vegetable production go waste during various steps of the post-harvest chain, smooth transport and insufficient cool storage space/ facility at farmer’s level and high cost of refrigerated storage so further enhances loss of fruits and vegetables. Temperature and humidity play major role in storage of fruits and vegetables Temperature can be controlled by using energy consuming methods such as air-cooling, hydro-cooling, vacuum-cooling, chilling, ice cooling, freezing, etc.

Food is a complex system of inorganic and organic compounds. These compounds are present in food in unique combination and uniquely organized structures, and therefore, every type of food has unique characteristics, nutritive value, sensory quality, texture, etc. Food items are always in physical, chemical and biological dynamics, however rate of change vary from food to food. Those highly perishable in nature undergo faster rate of spoilage as compare to those semi-perishable or stable. Foods are affected by nearly every variable of environment. Heat, cold, light or other radiations, oxygen, moisture/dryness, natural food enzymes, microorganisms, macro-organisms, industrials and agricultural contaminants, time, etc. - all adversely affect the foods resulting to its spoilage or deterioration. Spoilage may be referred to the losses in nutritive value, sensory desirability, safety and aesthetic appeal to the unacceptable level. Level of unacceptability is the subject of socio-economic status and policies of the state. A food which is of unacceptable quality for one society may be of acceptable quality for other society.

History reveals that food was the root cause of many wars and also the battles were won or lost over food spoilage and its control. Wars and the need to provide safe food to armies in the region far away from the areas of their production have always focused attention on the problem of food spoilage, and this valid today even. Some of the most important advancements in the technology of food preservation were made during the war-time. Of these, most important was the technology of preventing food from spoilage for months together developed by French scientist Nicolas Appert for French army. He won awarded money of 12,000 Francs in 1908 for this. The World learnt the art of canning foods in which the food is packed in a sealed metal container and heated sufficiently. He, however, could not explain the principle behind the preservation by canning. Some 50 years later, work of Louis Pasteur explained that the growth of microorganisms was the major cause of food spoilage which are killed in Appert’s method of preservation.

Fruits and vegetables are very important food commodities not only in developing country like India but all over the world. India, which is the most densely populated country in Asia continent, is still struggling to achieve self-sufficiency to feed about 135 crores people. For this purpose, fruits and vegetables have got their specific importance to provide a balance and healthy diet to the Indian people. Therefore, the reduction of post-harvest loss of fruit and vegetables is a complementary means for increasing production. It may not be necessary to considerably step up the production of fruits and vegetables with the growing demand if the post-harvest loss is reduced to a great extent. The cost of preventing losses after harvest in general is less than preventing a similar additional amount of fruit and vegetable crop of the same quality

Fruits are important source of Pro-vitamin A and vitamin C. In addition, vegetables also supply fair amount of carbohydrates, protein and energy and add colour, flavour and aroma to human diet. India is one of the largest producers of fruits and vegetables. Fruits and vegetables are an important supplement to the human balanced diet as they provide the essential minerals, vitamins and dietary fibre (roughage) for maintaining the tear and wear of the over body. Fresh fruit and vegetables have a short shelf life under ambient conditions of temperature and humidity due to their highly perishable nature. They soon lose their freshness and become subjected to mould and bacterial attack, and consequently decay and become useless as articles of human diet. Fresh fruits and vegetables are valued for their quick sources of available energy. Fresh fruits have high water content (70-96%), varying amount of carbohydrate (3-27%) and fibre (0.2-3.1%) and a low content of protein, fat and minerals. Ideal climatic conditions ensure round the year availability of broad range of fruit and vegetables in large quantities. The production of fruits and vegetables was 97.00 MT and 179.60 MT during the 2017-2018 in India

India is today the second largest producer of fruit and vegetable after China with a total annual production of 281.75 million tons in 2017-18. Fruit and vegetables are essential components of the human diet and nutrition constitute. Fruit and vegetable are also element of diversification in agriculture for additional income generating resource and employment generation area. Although India is the largest producer of fruit in the world, the production per capita is only about 100 g per day. Huge losses to the extent of 20 to 40% of the total production are reported due to lack of adequate infrastructure and transportation facilities and higher the transportation cost. Thus the per capita availability of fruits is further reduced to 60 -80 g per day which is almost half the requirement for a balanced diet. The fruit production in India has recorded a growth rate of 3.9%, whereas the fruit processing sector has grown at about 20% per annum. However, the growth rates have been extensively higher for frozen fruits and vegetables (121%) and dehydrated fruits and vegetables (24%).

Fruit and vegetables are mostly seasonal crops but they are demanded by consumers throughout the year. They are very highly perishable in nature, which is difficult to preserve for the longer period of time under ambient storage conditions. The low temperature storage retarded the activity of various biological and biochemical agents of spoilage and provides longer shelf life to the fruit and vegetables The temperature is brought down by taking out the heat with the help of mechanical refrigeration, This system is based on a liquid absorbing heat as it change to a gas. The more common mechanical refrigeration system use a refrigerant such as (Ammonia, Freon etc.,) takes out the heat from the chamber/ store as it is expand. The spoilage agents are microorganism such as (e.g., bacteria, yeast and molds etc.) and larva. Optimum storage temperature for most products is one slightly above the freezing point of the product. Some fruit and vegetables are particularly sensitive to storage temperature are susceptible to called storage disease when stored at temperature below as or above the critical temperature e.g.,

Packaging is essential for the safe and easy marketing of any food products and for the retention of their natural characteristics till consumed or utilized otherwise. Packaging is also an essential part of processing and distribution of foods. Packaging used for processed foods can contribute marketing to the retention of the quality characteristics. It must protect a variety of assaults including physical damage, chemical attack and contamination from biological vectors including microorganisms, insects and rodents. Environmental factors such as oxygen and water vapour will spoil foods if they are allowed to enter packages freely. Contamination of foods by microorganism can spoil foods or cause life-threatening diseases. With dehydrated food, packaging serves a multi protection role. Depending on the product being packed, packaging has to provide protection against moisture pick up, loss of volatile components, oxidation and damage of product itself. With the development of food industries, use of different types of plastic containers becomes widely popular owing to its several advantages.

Although packaging has, in recent years, been accused of using up valuable resources, it is nevertheless absolutely essential. Packaging is vital to protect and conserve the items contained within it. The dominant purpose of packaging is to protect the product so that it reaches the consumer in the same condition as when it was first made. However there is a lot more to the effectiveness and usefulness of packaging than just protection. When a consumer picks up their carton of freshly squeezed mango juice, they are not aware of the energy and money saved by the packaging they are grasping in their hands. In order to fully understand the essential role packaging plays in every day life, it is necessary to understand the guiding principles behind it.

Food is a major determinant of health, nutritional status and productivity of the population. It is, therefore, essential that the food we consume is wholesome and safe. Unsafe food can lead to a large number of food-borne diseases. It is necessary that the food available is safe, hygienic, wholesome and its quality must be assured. Quality of foods can be defined as the ‘composite’ of those ‘characteristics’ that differentiate individuals units of a product and have significance in determining the degree of acceptability of that unit by the buyer. In general, the quality control is the maintenance of quality at levels and tolerance acceptable to the buyer, minimizing the cost for the vender. But, from the scientific angle the overall quality refer to technical, physical, chemical, microbiological, nutritional and sensory parameters to achieve the wholesome food. These quality factors depend on the specific attributes such as sensory properties, based on flavour, colour, aroma, taste, texture, quantitative properties viz. percentage of sugar, protein, fibre etc and hidden attributes like peroxide, free fatty acids, enzymes etc. Though quality attributes are many, not all need to be considered for a particular product. It is imported to determine how far relatively the factor is in relation to the total quality of the product.

Hence, the quality attributes of a particular is based on the composition of the product, expected deteriorate reaction, packaging used, shelf required and the type of the consumer i.e., deployed in terrain conditions or in peaceful situation. With the ultimate goal of protecting the consumer, quality standardization systems have come into force for regulating the gamut of operations in food manufacture. In a broader sense, food laws and Regulation cover the related acts affecting the marketing, production, labeling, food additives used, dietary supplements, enforcement of General Manufacturing Practice (GMP), Hazard analysis and critical Control Point (HACCP), federal laws and regulations, factory inspections and important-export inspections.

The food is considered as one of the essential for the maintenance of life. Manufacturing of the food product is not enough form the food industry. It is necessary that the food available is safe, hygienic; wholesomeness and its quality must be assured. The food industry is an important segment of industries in India and has its own peculiarities unlike other industries. Food processing industry has been recognized by government as a major sector of growth. It must be free from harmful additives, microbes and remain so for a period, it is intended to be consumed. Therefore, the methods employed in quality and food safety assurance programme within the food industry will vary considerably in accuracy and sophistication according to the type of process and size and nature of resources available. Since the international market has become demanding in terms of quality, safety and delivery, installation of Hazard Analysis Critical Control Point (HACCP) in food industry would provide a competitive edge to food supplies in the international market. Food control refers to all activities carried out to ensure the quality, safety and honest presentation of the food at all stages from primary production through processing and storage, to marketing and consumption. The term has been used to describe a total national effort involving an integrated approach between government and all segment and sectors of the food industry. Food control is linked to improvement in the health of the population, potential of a country’s economic development and reduction of spoilage and food losses.

A wide variety of quality determinants is employed, including size, colour, maturity and extent of blemishes. Quality is a measure of the degree of excellence or degree of acceptability by the consumer. The marketing of fresh fruit and vegetables is aimed eventually ay appealing to the consumer for whom tradition plays a major role in determining the acceptability of a food item. The eating habits of people are conservative. The increase of direct market from producer to supermarket chains is leading to the imposition of standards to predetermined specification by the supermarkets. Important factors in quality for the consumers are: appearance, including size, colour, shape, condition and absence of defects, texture, flavour and nutritional value. Quality is the ultimate criterion of the desirability of any food product to the consumer. The overall quality of food depends on the nutritional and other hidden attributes. Quality of foods can be defined as the ‘composite’ of the ‘characteristics’ that differentiate individual units of a product and have significance in determining the degree of acceptability of that unit by the buyer.

The word nutrition first appeared in 1551 and comes from the Latin word nutrire, meaning “to nourish.” Today, we define nutrition as the sum of all processes involved in how organisms obtain nutrients, metabolize them, and use them to support all of life’s processes. Nutritional science is the investigation of how an organism is nourished, and incorporates the study of how nourishment affects personal health, population health, and planetary health. Nutritional science covers a wide spectrum of disciplines. As a result, nutritional scientists can specialize in particular aspects of nutrition such as biology, physiology, immunology, biochemistry, education, psychology, sustainability, and sociology.

Disease is defined as any abnormal condition affecting the health of an organism, and is characterized by specific signs and symptoms. Signs refer to identifying characteristics of a disease such as swelling, weight loss, or fever. Symptoms are the features of a disease recognized by a patient and/or their doctor. Symptoms can include nausea, fatigue, irritability, and pain. Diseases are broadly categorized as resulting from pathogens (i.e., bacteria, viruses, fungi, and parasites), deficiencies, genetics, and physiological dysfunction. Diseases that primarily affect physical health are those that impair body structure (as is the case with osteoporosis), or functioning (as is the case with cardiovascular disease). Mental illnesses primarily affect mental and social well-being.

The foods we eat contain nutrients. Nutrients are substances required by the body to perform its basic functions. Nutrients must be obtained from diet, since the human body does not synthesize them. Nutrients are used to produce energy, detect and respond to environmental surroundings, move, excrete wastes, respire (breathe), grow, and reproduce. There are six classes of nutrients required for the body to function and maintain overall health. These are carbohydrates, lipids, proteins, water, vitamins, and minerals. Foods also contain non-nutrients that may be harmful (such as cholesterol, dyes, and preservatives) or beneficial (such as antioxidants).

The foods we eat contain nutrients. Nutrients are substances required by the body to perform its basic functions. Nutrients must be obtained from diet, since the human body does not synthesize them. Nutrients are used to produce energy, detect and respond to environmental surroundings, move, excrete wastes, respire (breathe), grow, and reproduce. There are six classes of nutrients required for the body to function and maintain overall health. These are carbohydrates, lipids, proteins, water, vitamins, and minerals. Foods also contain non-nutrients that may be harmful (such as cholesterol, dyes, and preservatives) or beneficial (such as antioxidants). Nutrients that are needed in large amounts are called macro-nutrients. There are three classes of macro-nutrients: carbohydrates, lipids, and proteins. These can be metabolically processed into cellular energy. Micro-nutrients are nutrients required by the body in lesser amounts, but are still essential for carrying out bodily functions. Micro-nutrients include all the essential minerals and vitamins. There are sixteen essential minerals and thirteen vitamins.

Introduction

Fermentation is one of the oldest food preservation techniques that can be traced back thousands of years. Fermentation is referred as one of method of biopreservation as it leads to extending the shelf life of foods that involve the use of microorganisms and their metabolites. (Ross et al., 2002) For millennia, the fermentation of food by using micro organisms has been used as a technique for ensuring shelf life extension and improving the quality characteristics, texture and flavour of food products as well as to develop new innovative products. The major bacteria used in food fermentation worldwide are lactic acid bacteria (LAB). LAB is a multitude of genera including Lactobacillus, Lactococcus, Streptococcus, Leuconostoc, Pediococcus, and Enterococcus. Lactic acid bacteria constitutes a diverse group of bacteria that enjoys the status of “generally regarded as safe” (GRAS) and “qualified presumption of safety” (QPS) by the Food and Drug Administration (FDA) and European Food Safety Authority (EFSA), respectively. In this respect, starter microorganisms are known to produce a wide array of antimicrobial and protein-acoustic compounds to inhibit the growth of undesirable flora in foodstuffs resulting in shelf life extension. (Hill et al., 2017).

Introduction

Commonly available food materials especially dairy products, cereals and fruits are perishable and require processing for long term storage. The methods of food storage has been known from the pre historic times and was mostly based on processing the food into a more stable form with a longer shelf life. Such processes increases the time range of food availability and in many cases improves the taste thereby, cumulatively improving the food value. One of documented method of food preservation is by fermentation and is age old. Other commonly used methods include drying, refrigeration, salting and fermentation (Luck 1985, Paulus, 1985, Christensen et al., 2003, Martindale and Schiebel, 2017).

Flavour and appeal of food has been considered as one of the important factors contributing to the demand of the food. Originally the flavours were largely plant derived, current industrial practices largely utilise synthesis by chemical reactions and subsequent extraction to achieve a uniform quality (Weckerle et al., 2006, Davidovich-Rikanati et al., 2008, Sethupathy et al., 2018). These chemicals referred to as “flavourings” are defined as compounds that enhance the taste, appeal and palatability of food. They are naturally derived or artificially synthesised compounds. Considering that many artificially synthesised chemical flavours might affect the health adversely. Industrial food research has now shifted their focus on flavourings that are naturally available (Cohen et al., 2018). Such naturally occurring flavourings are referred to as bio-flavours (de Billerbeck 2015). The ease of producing bio active compounds from microbial culture extracts has played significant role in industrial food biotechnology (Verachtert et al., 2005).

Introduction

Fermentation is the process in which edible microorganisms invade food substrates and convert them into fermented foods including beverages, using their enzymatic and metabolic processes. These end products will have nontoxic flavors, aroma and texture which enhance the palatability. The word “fermentation” is originated from a Latin word fermentare, defined as “to leaven” (Frias et al., 2016). It is one of the oldest food processing technologies used by humans to preserve perishable food materials for long time, dated back to ancient human civilization (Hutkins, 2008). Globally, more than 5000 varieties of fermented foods are currently in use which includes traditionally fermented dairy products such as Dahi in India to industrial production of wines from fruits (Tamang, 2010) (Table 1 and Figure 1). Biochemically, fermentation is a metabolic process in which energy is derived from organic compounds without the involvement of an exogenous oxidizing agent. Fermentation may result in the production of many by-products such as carbon dioxide, alcohol, organic acids (lactic acid, acetic acid), vitamins, enzymes etc. which are now having more importance in food industries (Caplice and Fitzgerald 1999).

Introduction

Fermented foods are those foods which have been subjected to the action of microorganisms or enzymes that cause desirable biochemical changes and significant modification of the food (Campbell-Piatt, 1994). Fermented foods are usually produced using ingredients of plants or animals in combination with bacteria or fungi. The bacteria or fungi are either sourced from cultures maintained by humans or from the environment. The origins of fermented foods go back many thousands of years, with early evidence of the alcoholic fermentations of barley to beer and grapes to wine. Evidence suggests that fermented foods were consumed 7,000 years ago in Babylon (Battcock and Azam-Ali, 1998).Throughout the world, the fermented foods are used by the people. It plays a major role in the diet in many developing countries. Several traditional fermented foods are prepared in rural areas of many countries.

Introduction

Fermentation has been an integral part of food processing throughout the history of mankind. In beginning, it was mainly preservation method, then it became key process in substantial sensory characteristic development and now it is utilized to improve overall properties of outcome from major industries like bakery, dairy, beverages, etc. The human understanding of controlled fermentation process changes its fundamental objective by exploring its success in development of rheological and texture characteristics.

The biochemical transformation of raw food due to fermentation affects the organoleptic as well as rheological properties of a product. The changes in rheological as well as sensory properties is related with the modification of major component by the action of microorganism. For example, transformation of polysaccharides in bread dough by action of yeast produce ethanol, CO₂ and other simple compound which modify sensory as well as rheology of dough. The major micro-structural, bio-compositional and bio-chemical changes are due to microbial action are frequently described to understand the effect of fermentation on rheological as well as sensory properties of food products. The rate and quality of these associated major changes mainly depends upon concentration of saccharides for growth of microorganism as well as nature and existing microflorae. Hence, a proper monitoring of changes in rheological and sensory properties is necessary to optimize the controlled fermentation as well as design of process parameters from engineering and consumer point of view.

The biochemical changes directly responsible for changes in basic rheological and sensory characteristic including flow behavior, consistency, viscoelastic properties, hardness, adhesiveness, color, aroma, flavor and mouthfeel. The food industry is so diverse and exploring new and possibilities to catch diverse consumer worldwide, a proper understanding of effect of fermentation on mentioned properties will be key to hold competitive market. Hence, the present chapter will cover the basics of rheology and its role in food industry, effect of fermentation on rheological and sensory attributes of major class of food.

Introduction

Fermentation of food dates back to ancient practice of wine making. Fermented foods reflect culture and tradition of folklore and emphasis their close association with nature. Various methods employed for fermenting foods aimed in food preservation to reduce wastage; however lactic acid fermentation enhanced nutrition content and improved organoleptic properties. Experimental evidences on benefits on health and wellness on consumption of fermented foods are being reported. Fermentation process used for preparation of indigenous or traditional foods often depend entirely on natural microflora on the surface of raw material and containers used; Practices of fermenting foods are passed on as traditional know how to generations as a legacy. The fermented food manufacturers rely on commercial starter cultures, and highly reliant on indigenous materials as microbial collections are unique and highly variable according to region and products. Therefore, isolation of novel organisms, development of newer products and interactions are being explored using variety of fermented foods obtained through traditional processes

Introduction

Cereals are grown over 73% of the total world harvested area and contribute over 60% to the world food production providing dietary fibre, proteins, energy, minerals and vitamins required for human health. Cereals like rice, maize, wheat, barley, sorghum, and oats are chiefly used in the preparation of traditional fermented foods and beverages. However, the nutritional quality of cereals and the sensory properties of their products are sometimes inferior in comparison with dairy products. The reasons behind this the lower protein content, deficiency of certain essential amino acids, the presence of determined anti-nutrients (phytic acid, tanins and polyphenols) and the coarse nature.

Introduction

Eradication of malnutrition and hunger (Sustainable Development Goal, SDG-2) are the major challenges to humanity. Availability of sufficient nutritionally balanced food is the prerequisite for ensuring global food security. Food security is also critical for achievement of SDG 1 (poverty eradication) and SDG 3 (health and well-being) (UNDP, 2019). Changing climatic conditions accompanied by global warming not only acts a major threat for achievement of SDGs but could actually lead to the reversal of the progress already made. The escalating global temperature has jeopardized the food security throughout the world. A great decrease in the global crop yield has been suspected even with a 2°C rise in temperature. On field, it is often associated with crop nutrient loss, less accumulation of micronutrients and low availability of micro-minerals like iron and zinc. Additionally, the effect of the rainfall and temperature has also been observed. The global food prices are projected to increase by 3–84% by 2050 due to the fluctuations in rainfall and temperature (Hoegh-Guldberg et al., 2018).

Millets are the small seeded grains which belong to family Grassacae/Poaceae. Traditionally, millets are used for the food and feed purpose and are among the oldest cultivated crops. The millet species of commercial importance are proso (Panicum miliaceum), foxtail (Setaria italica), barnyard (Echinochloa esculenta), finger (Eleusine coracana), little (Panicum sumatrense) and pearl (Pennisetum glaucum) in different regions of the world. In India, foxtail millet (priyangava), barnyard millet (aanava) and black finger millet (shyaamaka) have been mentioned even in the Yajurveda texts of 4500 BC (Morya et al., 2017). The records of consumption of foxtail and proso millet are as old as 2000 to 1000 BC in China. Cultivation of foxtail millet in Korean peninsula is documented around 3,500–2,000 BC. Foxtail millet is the oldest of all the cultivated millets and is believed to be originated in Southern Asia. From there, it was spread to Europian nations. In the west, cultivation of millets majorly started after 1850 (ICRISAT, 2019)

Introduction

Foods prepared by fermenting raw ingredients of plant or animal sources naturally or mixing it with the starter culture (s) containing functional micro flora is called fermented foods. The functional micro flora used in starter culture is responsible for biochemical and organoleptic alteration of the substrates into edible yields which are socially and culturally appropriate to consumers (Tamang et al., 2010a, Tamang et al., 2010b). Fermented food has its own characteristic microflora and this group of micro flora increases the nutritional value by increasing the levels of essential amino acids, vitamins, proteins, etc.

Fermented foods play a major role in meeting nutritional requirement and socio economic development of developing countries. They are important components of the diet as staples, adjuncts to staples, condiments and beverages. A wide variety of fermenting substrates are available such as cereals, pulses, soya beans, flowers, milk, fish, meat, etc. A wide majority of plants and their parts are often utilized in the preparation of starter culture (Deori et al., 2007, Deka and Sarma, 2010).

Introduction

One of the main properties of fermented foods whether alkaline or acidic is the presence and activity of beneficial organisms which themselves make these foods functional. These functional micro organisms have varied roles like conversion of the native constituents of raw materials to forms in which they are more bioavailable, formation of taste, colour and flavouring compounds thereby imparting pleasantness to foods, removal of anti-nutritive factors in food by converting them into much less harmful compounds or degrade certain native toxic elements present in food, formation of biopreservatives which will extend the shelf life of foods and provide microbial security, as pathogens and spoilage microorganisms fail to readily grow in such hostile conditions. These functional microorganisms also produce in foods certain health-promoting bioactive compounds like enzymes, vitamins, minerals or increase the digestibility which may not have been in the original food or raw material before fermentation. In other words they improve the nutrition profile of the foods or naturally fortify foods. Some of these beneficial effects may also lead to the formation of certain compounds which show antioxidant properties in foods. These very roles played by these functional micro-organisms form criteria in their selection for use in food fermentations when they are intentionally added to foods both to initiate and accomplish desired fermentations.

Introduction

Over the past few decades, probiotics have become the most promising and emerging field in human nutrition and health across the world including India and other developing countries. The global profit generated from the probiotic market is expected to reach approximately 12,753.4 million US$ by the end of 2026 (Probiotic market report, 2018). The factor fueling the demand for probiotics is the growing awareness among consumers about their health benefits. The association of probiotics with health benefits stretches back to a time before microbes were discovered. However, the scientific evidence of the health benefits of probiotic microorganisms on the wellbeing of the host was firstly proposed by Russian Nobel laureate Ellie Metchnikoff in 1905. He reported that consumption of fermented milk containing Lactobacillus spp. is responsible for the healthy and long life of Bulgarian people (Anukam & Reid, 2007). Afterward, several of scientific progressions were made to reach the current status where probiotics are not only the subject of food and medical research but also the source of a multi-billion dollar global industry.

The term probiotic is derived from the Greek words, which means ‘for life’. According to the Food and Agriculture Association of the United Nations (FAO) and World Health Organization (WHO), probiotics are defined as “live microorganisms which when administered in adequate amount promote the health of the host” (Joint, FAO, 2002). In 2014, International Scientific Association for Probiotics and Prebiotics (IPPA) did minor correction in the definition given by FAO-WHO and defined them as “Live microorganisms that, when administered in adequate amounts, confer a health benefit on the host”

Introduction

Food is the most vital prerequisite for sustenance of human life and provides adequate nutrients for the metabolism. Recently several hypothesis supports that, beyond meeting nutritional needs, they play beneficial roles in improving health, and minimize the risk of illnesses (Roberfroid, 2000a). In this way, a novel term “functional food” was introduced and plays an outstanding role in improving the overall conditions of the human body (e.g. pre- and probiotics), lessening the threat of some ailments (e.g. cholesterol-lowering products), and also as therapeutics for several diseases (Roberfroid, 2000a, Roberfroid, 2000b, Mollet and Rowland, 2002).

Recently throughout the world, an extensive array of functional foods were developed which includes prebiotics, probiotics, synbiotics, foods supplemented with anthocyanins, phytosterols, isoflavones, antioxidants, and fat, sugar and salt-reduced foods. Amongst them, probiotic functional food has exerted beneficial effects on the health and is comprised of both probiotic dairy foods and probiotic non-dairy foods. The demand for dairy probiotic foods are increasing as it attributes to the beneficial health effects on the host by improving the survival of gastrointestinal microflora, production of bioactive compounds and prevention of lactose intolerance. Consequently, production of these foods is a significant research importance for both food manufacturing units and research institutes.

Probiotics are the important ingredients for foods with a “healthy” image. It could be combined with other beneficial ingredients or simply used to supplement the natural functional qualities of whole foods. The amalgamation of probiotic bacteria with nutrient rich foods, like dairy products, will have the additional value of improving consumer nutrition.

Introduction

Fermented meat and fish products have been consumed all over the world since times immemorial and represent one of the most important groups of food. Meat offers its own natural microflora. Hence a wide variety of products have been prepared since ancient times by varying the mixture of meat and salt as well as by the addition of spices and seasonings. Thus, fermented meat products characterize a diversity of flavors and textures and are receiving increased interest from consumers all over the world. Most of these products still depend mainly on local, traditional manufacturing processes since little scientific information is available; but scientific knowledge is very important for consistent production of high quality and safe products.

Meat is an essential part of non-vegetarian diet. It is liked for its unique taste and is a rich source of nutrients like good quality animal proteins, essential amino acids and fatty acids, minerals, trace elements and particularly B-complex vitamins (Singh et al., 2013). The growing concern of consumers regarding health and safety issues has led to the development of functional meat products that promote health and well-being, beyond their nutritional benefits (Grasso, et al., 2014; Tyopponen et al., 2003). This has driven researches in the meat processing sector to increase their efforts in producing such functional meat products, which have incorporated functional ingredients, reduced salt, nitrates/nitrites, cholesterol and fat and modified product fatty acid profiles (Zhang et al., 2010). Fermentation of meat produces a wide range of physical, biochemical and microbial changes in the muscle-based product