eChapters

Introduction

The term biological control was first used in scientific literature in 1914. Baker and Cook (1974) described biological control as “reduction of inoculum density or disease producing activities of a pathogen or parasite in its active or dormant state, by one or more organisms, accomplished naturally or thoroughly manipulation of environment, host, or antagonists or by mass introduction of one or more antagonists.”

Certain genera of fungi have greater potential for being effective bio-control agents eg. Trichoderma, Gliocladium, Chaetomium; entomogenous fungi as Entomophthora, Beauveria, Metarrhizium, Hirsutella; Nematophagous viz., Catenaria, Zoopage, Stylopage, Paecilomyces and predaceaus fungi like Arthrobotrys, Dictylella, Dactylaria and Nematoctonus (Narain, 2018). Biopesticide formulations based on these fungi are also called as “Microbial pesticides”.

Proximate Analysis of Biomass
Objective
To determine the key compositional parameters of biomass, including moisture content, volatile matter, ash content, and fixed carbon, using standardized analytical procedures.
Principle
Proximate analysis of biomass is a standardized method used to determine the basic compositional components of biomass materials. It provides essential information about fuel quality and conversion characteristics. Biomass can serve as an energy source either directly through combustion or indirectly through conversion into gaseous, liquid, or solid fuels for more efficient utilization. These transformations occur through thermochemical processes (combustion, gasification, and pyrolysis) and biochemical processes (anaerobic digestion and fermentation).
Common biomass materials such as agricultural residues, cattle dung, poultry litter, and paddy straw are analyzed for parameters including total solids (TS), volatile solids (VS), ash content, total organic carbon (TOC), moisture content, and total sugars. These parameters are typically estimated using AOAC (2000) and other standard protocol.
Requirements
Biomass samples (such as agricultural residues, cattle dung, poultry litter, paddy straw and others materials from living organisms but now dead), silica crucible, hot air oven, desiccator, muffle furnace, weighing balance, 5% Phenol, concentrated H2SO4, UV-VIS spectrophotometer, vortex mixer, glassware like beaker and test tubes

Introduction

Chickpea (Cicer arietinum L. ) popularly known as Gram, Bengal gram, Egyptian pea, Chana, or Kabuli chana, Garbanzo bean is one of the first grain legumes to be domesticated by humans in old world (Van der Maesen, 1972). The genus Cicer belongs to the sub-family Papilionaceae of the family Leguminoceae (Bentham and Hooker, 1970) now popularly known as ‘Fabaceae’ and have chromosome number 2n = 2x = 16. The origin of the crop is considered to be Western Asia from where it has been transferred in India and other parts of the world (Rathore and Sharma, 2003). Importance of pulses is relatively more in our country as its contribution in nutrient supply is far more in Indian diet than that in Asia and world as a whole (Ali,2002).

Fig. 25: ICPH 2671 in field

Raman Spectroscopy

It is a spectroscopic technique based on inelastic scattering of monochromatic light, usually from a laser source. Inelastic scattering means that the frequency of photons in monochromatic light changes upon interaction with a sample. Photons of the laser light are absorbed by the sample and then reemitted. Frequency of the reemitted photons is shifted up or down in comparison with original monochromatic frequency, which is called the Raman effect. This shift provides information about vibrational, rotational and other low frequency transitions in molecules. Raman spectroscopy can be used to study solid, liquid and gaseous samples.

1. Introduction

Ornamentals include plants which have either attractive foliage or flowers. They are used for various purposes like bedding plants in the garden or as cut-flowers and have huge commercial importance in terms of nursery business. New cultivars of ornamentals, which have been developed by various plant improvement techniques (hybridization, mutation breeding and colchiploidy) are always an added attraction to the plant lovers and a highly prized commodity for commercialization.

New cultivars of ornamentals usually inherit characters of both the parents singly or in combination and produce an entirely new trait. The progenies, thus, express a range of phenotypic characters. One has to screen these plants and identify elite plant(s) having desired phenotypic characters.

Second part of this process is characterization of the selected plants. Various characters of these plants are studied as per laid down procedures, which include vegetative, floral, seed and rhizome characters. The recorded observations are then compared with the parental characters to establish their distinctiveness. Subsequently, these plants are subjected to field trials for studying their uniformity and stability of the desired phenotypic characters.

New cultivars of ornamentals have enough potential for commercialization and good source of earning on the part of breeders who may be farmers, nurseryman, and researchers. However, for claiming right over the new cultivars developed, it is now mandatory to get those new varieties registered with appropriate agencies. The registration authorities ask for every morphological details as well as parental history. Therefore, characterization is a must for the newly developed cultivars/varieties.

ABSTRACT

Atotal of 45 Gram positive, spore forming and rod shaped bacteria identified as Bacillus species were isolated from the rhizosphere of Chickpea plants. They were studied for their interaction with root borne fungal pathogens was studied using spot test method. Isolates CBS 29, CBS 113, CBS 123, CBS 132, CBS 153 and CBS 155 inhibited the growth of four or more fungi with inhibition zones varied from 1-5 mm. Two isolates CBS 153 and CBS 155 produced siderophores on CAS supplemented agar medium. Further inoculation of these Bacilli on seeds of Chickpea cultivar C235 and H8618 showed inhibitory, stunting or stimulatory effects on plumule and radicle growth depending upon cultivar, bacterium used and stage of seedling growth. Studies on co-inoculation of these isolates with Mesorhizobium and their plant growth promoting activity are being carried out. These co-inoculation studies are important areas of exploitation to improve crop productivity.

6.1 Characterization of solid pyrolysis products

 Biochar can be characterized for its utility as well as its energy usage. Thevarious characteristics of biochar can be categorized as follows:

i. Proximate analysis

 •            Raw moisture

 •            Hygroscopic moisture

 •            Volatile matter

 •            Ash content

 •            Fixed carbon content

 ii. Ultimate analysis

 •            Elemental compositions (C, H, N, O, S)

 iii. Physical properties

 •            True density

 •            Bulk density

 •            Porosity

 •            Dry matter

 •            Particle size

 •            Pore size

 •            Pore volume

 iv. Chemical properties

 •            pH-value

 •            Electrical conductivity

 •            Carbonate content

The nuclear magnetic resonance spectroscopy (NMR) has led to major advances in the studies on the nature and chemical composition of soil organic matter. The technique has the potential for characterizing organic matter in the intact soil and its size fractions without the need for extraction and fractionation. Furthermore, it can provide information on the compositional changes in crop residues, peat and litter of the forest soils during biodegradation and humification.

Humic substances, the natural organic materials, represent a mixture of relatively small organic components, which form supramolecular structures held together by dispersive forces such as n - n and van der Waals’ interactions. Identification of the best analytical method for complete characterization of humic substances is still being discussed. Humic substances differ in molecular weight, elemental composition, acidity and cation exchange capacity and often are classified into three major fractions according to their solubility as humic acids, fulvic acids and humins.

In India the problems of protein energy malnutrition, anemia, and vitamin A deficiency are more prevalent among children and adolescents (Udayasree, Manjula, & Sowjanya, 2013). To overcome these problems, the need of nutritional compositions rich foods came into existence. One of such foods which constitute the most significant attention of nutrients is “Spirulina”. Spirulina is a common name for supplements derived mainly from two Cyanobacteria species, i.e. Arthrospira maxima and Arthrosepira platensis, which are human and animal.

Characterizing agri-biomass and organic waste involves various methods to determine their composition and properties. These methods are crucial for optimizing their utilization in processes like bioenergy production, biochar generation, and soil amendment. Characterizing agri-biomass and organic waste is essential for determining their suitability for valorization processes such as composting, anaerobic digestion, pyrolysis, or biofuel production. Proper characterization provides insights into the physical, chemical, biological, and energy-related properties of the waste, which influence its treatment methods, energy potential, and environmental impact.
Characterizing Agri-Biomass
1. Physical Characterization
• Moisture Content: Indicates water content, affecting energy yield, microbial degradation, and storage. Measured using drying ovens or moisture analyzers.
• Particle Size and Bulk Density: Influences handling, processing, and transport efficiency, especially in thermochemical processes.
• Total Solids (TS) and Volatile Solids (VS): TS refers to all solid matter, while VS denotes the biodegradable organic portion of the waste.
2. Chemical Characterization
• Carbon-to-Nitrogen Ratio (C/N Ratio): A vital parameter for microbial
processes. An optimal ratio (typically 25–30:1) supports composting and
anaerobic digestion.
• Elemental Analysis (CHNS/O): Determines percentages of carbon, hydrogen, nitrogen, sulfur, and oxygen, important for estimating energy content and emissions.
• Ash Content: Indicates inorganic residue post-combustion. High ash reduces energy value and may affect equipment performance.
• pH Value: Critical for biological treatments. Most microbes thrive in a neutral pH range (6.5–7.5).

Bibliometric Analysis

1. What is?

Bibliometric analysis is a quantitative research method used to examine and analyze the bibliographic data present in scientific publications.

2. It involves?

The systematic evaluation of publication patterns, citation relationships, and other bibliographic characteristics to gain insights into the scholarly landscape.

3. How?

By applying various metrics and indicators, bibliometric analysis helps researchers and institutions assess research impact, identify emerging trends, map collaborations, and make informed decisions.

The term pesticide includes fungicides, bactericides, nematicides, rodenticides, soil fumigants and herbicides. They cover a wide range of formulations: chlorinated hydrocarbons, organophosphates, metallic salts, acetic acid derivatives, thiocarbamates etc. They all have deleterious effects on humans, domestic animals, wildlife and plants.

Cheese is a milk product of ancient origin and portrays the ethos of civilization and its cultural evolution. Cheese is one of the oldest foods of mankind. It is commonly believed that cheese evolved in the Fertile Crescent between the rivers Tigris and Euphrates in Iraq some 8000 years ago. The so-called Agricultural Revolution occurred here with the domestication of plants and animals.

It seems that the cheese originated accidentally as a result of the activities of nomadic tribes. Since animal skin bags were a convenient way of storing liquids for nomadic people, these were used for storing surplus milk. Fermentation of the milk sugars in the warm climate prevailing would cause the milk to curdle in the bags. The swaying animals would have broken up the acid curd during journeys, to produce curds and whey. The whey provided a refreshing drink on hot journeys, while the curds, preserved by the acid of fermentation and a handful of salt, became a source of high protein food supplementing the meager meat supply.

This activity gave rise to the assumption that cheese was evolved from fermented milks. It is perhaps more probable that the crude fermentations progressed in two ways. In one direction towards the production of liquid fermented milks such as dahi, yoghurt, laban, koumis and Kefir and in the other direction through the drainage of whey through a cloth or perforated bowls, to leave solid curds which when salted became cheese.

It was a prominent article of the Greek and Roman diet as much as 2500 years ago. It is referred to in the Old Testament several times.

19.1 Introduction
Cheese is a product that is produced by coagulating curd from milk with rennet or similar enzymes in the presence of lactic acid produced by adventitious or added microorganisms. Following the shaping of the curd in a mould and the removal of a portion of the moisture, it is permitted to mature by being maintained at an appropriate temperature and humidity for a specified duration.The FSSAI defines cheese as soft, semi-hard, hard, or extra-hard products that are matured or unripened, have a whey protein to casein ratio that is not greater than that of milk, and can be coated with food-grade waxes or polyfilm. coagulating agents. During this procedure, flavor-producing bacteria and harmless lactic acid bacteria may be introduced. The separation of whey is facilitated by the coagulation that results, which also ensures that the cheesemaking process concentrates milk proteins, particularly casein. Consequently, the protein content of cheese is substantially higher than that of the initial milk material blend. Furthermore, cheese-making incorporates methods that result in a final product with comparable physical, chemical, and sensory attributes by coagulating milk proteins or milk-derived products. The ingredients of cheese are limited to those that are naturally present in milk, with the exception of certain additives such as coagulating agents, sodium chloride, and small quantities of calcium chloride, annatto or carotene colourants, and specific emulsifiers and stabilisers such as citric acid, sodium citrate, sodium salts of orthophosphoric acid, and polyphosphoric acid. The wax employed for coating must not present any health hazards, and if it is coloured, it must be made from only approved food colours. The moisture content of hard cheeses is determined by the presence of no less than 42% milk fat and no more than 43% moisture on a dry matter basis. Additionally, they may contain trace quantities of nisin and ascorbic acid or its salts.

Sauropus androgynus, also known as katuk, star gooseberry, or sweet leaf, is a tropical shrub that is used as a leaf vegetable in some areas. It’s known as Chinese Malunggay in China, and madhura cheera in Kerala, India. It has several upright stems that can grow up to 2.5 metres tall and dark green oval leaves that are 5-6 cm long. It’s known as ‘Multivitamin Greens’ because of its great nutritional value. Chekurmanis, which may be found in practically every household kitchen garden in Kerala, has not been cultivated for commercial use on a significant scale. As a result, there are no data on the area and production of these vegetables.

The term “chelate” comes from the Greek word “chel,” meaning “claw,” indicating that the element is tightly held or clawed by the ligand. The term was coined by Morgan and Drew in 1920, defining it as a chemical compound in the form of a heterocyclic ring with a metal ion attached by coordinate bonds to at least two non-metal ions, known as chelation. A chelate is a type of mineral complex, which is a mixture of a mineral and an organic compound like a protein or polysaccharide. Chelation refers to the bond formed between a metal ion (mineral) and a ligand (protein or amino acid chelating agent). Chelates are created by reacting a mineral salt with an enzymatically prepared mixture of amino acids and small peptides under controlled conditions. The ligand binds the metal at multiple points, forming a ring structure with the mineral, resulting in a chelate.
In animal feed, chelated minerals, such as iron, manganese, cobalt, copper, and zinc, are commonly used to enhance bioavailability and support metabolic functions. These trace elements are transitional elements on the Periodic Table, forming stable complexes or chelates due to their ability to form coordinate covalent bonds.
The primary goal of chelates is to improve mineral absorption in animals during periods of high nutritional demand, such as pregnancy, lactation, weaning, and environmental or disease stress. Chelates have been studied for their effects on immunity, reproductive performance, and overall herd health. To maximize mineral absorption, chelated compounds should be stable in the rumen.
Chelates are stable, electrically neutral complexes that protect trace minerals from chemical reactions during digestion, ensuring their availability to the animal. Chelated minerals are used to increase bioavailability and uptake, reaching the plasma intact and separating at the site of action. They can replace a portion of inorganic minerals in animal diets and are suitable for all species. Soluble chelated trace minerals are designed for use in drinking water supplies.

Objective
To determine the chemical composition of biomass in terms of cellulose, hemicellulose, lignin, and silica content using standard detergent fiber analysis and acid digestion techniques.
Principle
Chemical analysis of biomass helps in evaluating its structural and compositional characteristics, which influence its thermal behavior, degradation properties, and suitability for bioenergy applications. Biomass primarily consists of cellulose, hemicellulose, lignin, and pentosans, each playing a distinct role in the structure and energy potential of plant material:
Cellulose – A linear polymer of ß-D-glucose units linked by ß-(1?4) glycosidic bonds. It provides tensile strength and rigidity to plant cell walls and serves as the main carbohydrate source in biomass.
Hemicellulose – A heterogeneous branched polymer composed of pentoses (xylose, arabinose) and hexoses (mannose, glucose, galactose). It acts as a matrix surrounding cellulose microfibrils, providing flexibility and binding lignin and cellulose together.
Lignin – A complex three-dimensional polymer made up of phenylpropane units. It is highly resistant to microbial degradation, provides compressive strength, and acts as a natural binder in plant tissues.
Pentosans – Polymers of five-carbon sugars that contribute to carbohydrate storage and structural integrity.

Preparation of Samples

Before withdrawing portions for analytical determinations, bring the sample to a temperature of 26 to 28ºC and mix throughly by pouring gently into a clean dry receptacle and back, until a homogenous mixture is assured. If lumps of cream do not completely disappear, warm the sample to about 40ºC, mix throughly, then cool to 26 to 28ºC. In case a measured volume is required in a determination, bring the temperature of the sample to about 27ºC before pipetting. All samples shall be allowed to stand for three to four minutes after mixing to allow air bubbles to rise ; the sample bottle shall then be inverted three or four times immediately prior to taking the milk for analysis. If a sample is curdled, excessively churned or decomposed, the sample should not be taken up for analysis.