eChapters

Chrysomelid beetle : Aulacophora foveicollis : Chrysomelidae

Adults cut tender shoots and feed on leaves resulting in holes and loss of leaf. Grubs feed on roots and underground stems leading to yellowing,wilting and drying of attacked plants.

Introduction

China aster is one of the most popular annual flowering plant grown throughout the world. Among annuals it ranks next to chrysanthemum and marigold. It can easily be grown in the open fields for cut and loose flowers. Cut asters last long and are used in vases and floral decoration. When asters are grown in large mass, they make very showy bedding plants and are valuable for filling up the gap in mixed herbaceous border. The dwarf types are put in front and the taller behind.

Some varieties are used as pot plants. The prominent colours of flowers are blue, violet, lavender, rose, white, etc. The commercial importance of China aster is increasing in India especially in Karnataka, Tamil Nadu, West Bengal and Maharashtra.

Genetics

China aster (Callistephus chinensis L. Nees) belongs to family Asteraceae. The genus Callistephus has only a single species i.e. C. chinensis. The ploidy level is diploid (2n = 18).

Abstract: The most popular ornamental monotypic species chinensis, is an important annual flower, used commercially as cut flower for interior decoration, bedding, potting and landscape purpose. Currently, there are many interspecific hybrid cultivars from this species on the market. The influence of breeding methods on the selection criterion and selection procedures is discussed. Information on variability and correlation is included. Ample genetic information and trait heritability has been established for this crop. Traits of interest to flower breeders for crop transformation include plant architecture, flowering (colour, size, timings), attractive flower heads, extended bloom time, horizontal placement of flowers, floral longevity, earliness, healthy foliage or other morphological traits. Future crop ideotypes are proposed to continue transformation of this crop for large scale cultivation.

The earliest Brassica planted in North America was Chinese cabbage, also known as celery cabbage and napa. It is becoming increasingly popular in western kitchens. When eaten fresh, the huge savoyed leaves with thick succulent midribs have a sweet flavour and crisp texture. The Pekinensis Group and the Chinensis Group are two cultivar groups of leaf vegetables frequently used in Chinese cuisine. These vegetables are turnip cultivars or subspecies that belong to the same genus as cabbage, broccoli, and cauliflower, among other Western mainstays.

Botany & taxonomy: Brassica rapa, one of two commonly cultivated mustard family (Brassicaceae) members that are variants of Chinese cabbage. Pak Choy is a type of Chinese cabbage (Brassica rapa var. chinensis). Celery cabbage is another name for Napa cabbage.

Leaf-and shoot folders: Hymenia recurvalis : Pyraustidae

Pycnarmon cribrata;Phostria piasusalis

Chironji or Charoli (Buchanania lanzan Spreng.) is an important fruit tree of agro forestry and social forestry. In the wasteland development and dryland horticulture, it assumes great significance due to its multifarious uses and capacity to withstand adverse environmental conditions. At present, it is growing under natural forest as an underexploited fruit and gives monitory reward to the tribal community of the country and seems to be boon for them. It is valuable tree species found in dry deciduous forest throughout the country excluding eastern Himalayan forests (Singh, 1982).

Chironji or Charoli (Buchanania lanzan Spreng Family: Anacardiaceae) is an excellent tree of agro forestry and social forestry. In wasteland development and Dryland horticulture, it assumes great significance due to multifarious uses and capacity to withstand adverse climatic conditions. At present, it is growing under forest condition as an under exploited fruit and gives monitory reward to tribal community of country and seems to be a boon for them. It is a valuable species found in dry deciduous forest throughout the country excluding eastern Himalayan forests (Singh, 1982). Flesh of ripe fruit is very palatable and is largely eaten raw or roasted and oily kernels are the most important part and are used in preparation of sweet meats and puddings (Singh, 1982). Mesocarp of fruit is edible and cherished by children (Munde et al, 2003). Kernel is highly nutritious and rich in protein (18.00 %) and yields sweet oil, which can be used to substitute olive and almond oil. Kernel contains 33.50 % oil, 1.90 % of which is unsaponifiable. Saponifiable part contained 20.00 % of linoleic acid. Chironji oil is non-repellant and non-toxic and is suitable for human consumption (Banerjee and Jain, 1988). Hemawathy and Prabhakar (1988) reported that total kernel lipid extracted from chironji was 65.60 % of dry kernel and consisted of 90.40 % neutral lipids, 3.4 % glycolipids and 6.2 % phospholipids. Kernel can be used for preparation of sweets. It provides quality timber wood for various uses.

Abstract

Chitin has been ranked at second position after cellulose as the most abundant natural biopolymer. Chitin has similar chemical structure to that of cellulose with 2-acetamido-2-deoxy-b-d-glucose (NAG) monomers attached via ß (1–>4) linkages. Chitosan is the deacetylated (to varying degrees) form of chitin, which, unlike chitin, is soluble in acidic solutions. Therefore, Chitosan, a copolymer of glucosamine and N-acetyl glucosamine, is amongst the most copious biopolymers on earth and finds wide range of biomedical and industrial applications. It has been approved in 1983 by USDA as a feed additive. Chitin basically is obtained from prawn/crab shells and chemical treatment of chitin produces chitosan. Since supplies of seafood waste are seasonable and variable, so fungi can be used as alternative source for chitosan. The unique physicochemical properties of chitosan provide great potential of its utilization in food industry especially dairy industry such as production of dairy products, dairy waste water treatment, extension of shelf life of dairy products etc. In recent years, exotic synthetic compounds are losing their appeal, thus application of chitinous products in dairy as well as processing aids has received considerable attention. The modified chitosan with greater functional properties has increased application in different dairy foods. This chapter focuses on the fundamental uses of chitosan and their derivatives in a variety of dairy industry applications.

Chlamydiae are obligate intracellular bacteria with an unusual developmental cycle during which unique infectious forms are produced. They replicate within cytoplasmic vacuoles in host cells. On account of their apparent inability to generate ATP, with resultant dependence on host cell metabolism, they have been termed ‘energy parasites’.
The family Chlamydiaceae belongs to the order Chlamydiales. Currently two genera, Chlamydia and Chlamydophila, and nine species are described. Formerly a single genus and four species, Chlamydia trachomatis, C. psittaci, C. pneumoniae and C. pecorum, were recognised. This classification was based on phenotypic characteristics such as host preference, inclusion morphology, iodine staining for the presence of glycogen, and sulphonamide susceptibility. However, recent nucleic acid sequencing studies of the 16s and 23s rRNA genes confirm two distinct lineages.
In the developmental cycle of chlamydiae, infectious and reproductive forms are morphologically distinct. Infectious extracellular forms, called elementary bodies (EBs) are small (200 to 300 nm), metabolically inert and osmotically stable. Each EB is surrounded by a conventional bacterial cytoplasmic membrane, a periplasmic space and an outer envelope containing lipopolysaccharide.
The periplasmic space does not contain a detectable peptidoglycan layer and the EB relies on disulphide cross-linked envelope proteins for osmotic stability. Elementary bodies enter host cells by receptor-mediated endocytosis. Acidification of the endosome and fusion with lysosomes are prevented by mechanisms which are not fully understood. A process of structural reorganization within the pathogen, of several hours duration, results in the conversion of an EB into a reticulate body (RB). The RB, about 1 μm in diameter, is metabolically active, osmotically fragile and replicates by binary fission within the endosome. The endosome and its contents, when stained, is called an inclusion. When a number of inclusions containing RBs of C.trachomatis are formed in an infected cell, fusion of these structures may
occur.

The word ‘chlamydia’ comes from the Greek word ‘chlamys’ which means, “cloak draped around the shoulder”. This describes how the intracytoplasmic inclusions caused by the bacterium are “draped” around the infected cell’s nucleus. Chlamydia was first discovered by a Czech zoologist and parasitologist, Stanislaus von Prowazek in 1907 in Berlin, but scientists believe it was actively infecting people for centuries before its discovery. Because the symptoms of Chlamydia are similar to some other infections it was not identified as a sexually transmitted disease. Originally, because of its structure it was thought to be a virus. In 1963 it was recognized as bacteria rather than a virus.

Properties of genus

           They are poorly stained by Gram’s stain. Chlamydiae are readily stained by Castaneda (blue in appearance), Giemnez and Macchiavello (red in appearance) methods. However, Giemsa staining is preferable to stain the inclusions (Chlamydial micro colony) in the cell culture. Inclusion bodies of the Chlamydiae are basophilic in nature. Again, the staining of the inclusion bodies may differ depending on the species. In C. trachomatis, the inclusion bodies contain glycogen matrix and can be easily stained with iodine solution.

Chlamydial organisms are spherical 0.2-1.5 um in diameter. They can be stained and seen in the light microscope, but they grow only in living cells. Chlamydial organisms infect mammals and birds causing various disease syndromes. Chlamydiacae family has one genus; Chlamydia. C. trachomatis, C. suis and C. muridarum. They cause disease in man, swine and mice. C. psittaci, C. pneumonia, C. felis, C. Caviae, and C. abortus. cause disease in man, birds and animals. Species can be differentiated on their growth characters inside the host cell.

Principle
Chloride ion is determined by Mohr’s method, titration with standard silver nitrate solution in which silver chloride is precipitated first. The end of titration is indicated by formation of red silver chromate from excess AgNO3 and potassium chromate used as an indicator in neutral to slightly alkaline solution.
AgNO3 +Cl- ? AgCl+NO3
2AgNO3+ K2CrO4 ? Ag2CrO4+2KNO3 (Reddish Brown)
Reagents
1. Standard Silver nitrate 0.0141N : Take 1.189 g and dissolved it in 500 ml distilled water.
2. Sodium chloride 0.014N: Dissolve 0.28 g sodium chloride in 500 ml of distilled water.
3. Potassium Chromate indicator
Procedure
1. Take 50 ml sample in a 100 ml conical flask
2. Adjust its pH in between 7.0 and 8.0 either with alkali or acid solution. Otherwise, AgOH is formed at high pH level or CrO4 -2 at low pH level.
3. Add 1 ml of potassium chromate (K2CrO4) colour turn to light yellow.
4. Titrate with standard silver nitrate (AgNO3) solution till colour change from light yellow to brick red colour.
5. Note the volume of AgNO3 added (A)
6. For better accuracy, titrate distilled water in the same manner
7. Note the volume of silver nitrate added for distilled water (B)

Mutation in M₂

The output of mutations in M₂ and subsequent generations solely depends upon the complex interaction between many factors in the treated organism together with the specific modes of action of particular mutagen. These results are expressed quantitatively by the mutation rate and qualitatively by the mutation spectrum. The proportion of mutated plants to normal ones which is the mutation rate can be computed on the number of mutations per 100 M₁ plants (Gustafsson, 1940), number of mutations per 100 M₁ spikes (Stadler, 1928), and number of mutants per 100 M₂ plants (Gaul, 1960). After comparing the three indices, Gaul (1960) has concluded that mutants per 100 M₂ plants provided the best index, as it is proportional to the initial mutation rate and is independent of variations in progeny size and size of mutated sector. In Pisum, Blixt (1966) has confirmed this. Mohan Rao (1972) has reported that in barley, the M₂ seedling method determined the total induced mutation frequency. This method is also superior since it accounted for the components of induced mutation frequency viz., the frequency of mutated initials in each spike primordium and the frequency of spike primordia mutated.

The qualitative output of mutation in M₂ and later generations is expressed as the mutation spectrum which reflects the different frequencies of different mutations. Westergard (1960) found that the alkylating chemicals induced a higher proportion of less drastic mutations such as Viridis compared to extreme mutations such as Albina than those induced by radiations because of their apparent slight effect on chromosomes. Sidorova (1966) found a positive correlation between the spectrum of chlorophyll and the spectrum of morphological mutations.

Abstract

Genus Chlorophytum is represented by approximately 300 species of rhizomatous plants, distributed predominantly in tropical and sub tropical forests of the world. Most of these are medicinally important. Chlorophytum borivilianum Santapau & R.R. Fern among these is highly valued because of high saponin content mainly concentrated in its tuberous roots. These steroidal saponins are important bioactive components responsible for aphrodisiac attributes of this species in addition to several other benefits. Present communication reviews the data available on medicinal properties and phytochemical constitution of the species.

Introduction

Increasing production of cereal crops, oil seed crops, and horticultural crops is becoming more important than ever before owing to increasing population and changing food habits of the people. The modern tools of biotechnology are being exploited to the fullest throughout the world for developing transgenic crop plants with improved traits. Already about 20 countries in the world are growing transgenic crops, predominately with traits such herbicide tolerance and resistance to insect-pests. However, there is a constant fear of pollen flow from theses transgenics to the neighbouring non-transgenic fields or to their weedy or wild relatives. It has become a matter of great concern to all biotechnologists to develop alternative strategies to prevent the pollen flow.

The best alternative strategy available today to circumvent the nuclear- transformation associated bottlenecks in developing tenable crop transgenics is the chloroplast transformation technology. This technology allows introduction and insertion of foreign genes into the chloroplast genome, called plastome. Chloroplasts are the site for photosynthesis in plants mostly present in leaves. In general called plastids, they are also present in other parts of the plant. For example, chromoplasts in coloured leaves and fruits, amyloplasts in starch storing organs, leucoplats in roots, and etioplasts in dark-grown seedlings. The plastids arise from proplastids - the undifferentiated form of plastids present in most plant cells. Just like nucleus, these plastids (and also mitochondria) contain their own genetic material on the chloroplast genome.

Chocolate is almost unique as a food in that it is solid at normal room temperatures yet melts easily within the mouth. This is because the main fat in it, which is called cocoa butter, is essentially solid at temperatures below 25^oC when it holds all the solid sugar and cocoa particles together. This fat is, however, almost entirely liquid at body temperature, enabling the particles to flow past one another, so the chocolate becomes a smooth liquid when it is heated in the mouth. Chocolate also has a sweet taste that is attractive to most people.

The first known cocoa plantations were established by the Maya in the lowlands of south Yucatan about 600 AD. Cocoa trees were being grown by the Aztecs of Mexico and the Incas of Peru when the Europeans discovered central America. The beans were highly prized and used as money as well as to produce a drink known as chocolatl. The beans were roasted in earthenware pots and crushed between stones, sometimes using decorated heated tables and mill stones, similar to those illustrated in Figure 1.1.

The development of a recipe for chocolate should be based on three criteria:

(i) The intended market or use;

(ii) The limitation being placed on material and production costs;

(iii) The desired quality.

The amount of fat present in the chocolate will affect the flow properties, and fat content must therefore be varied according to the intended use.

Some point to remember while manufacturing chocolate:

1. To maintain proper viscosity if fat content is increased then sugar content should also be increased.
2. Milk chocolate requires lower fat content than dark chocolate.
3. Addition of emulsifier like commercial lecithin can save upto 5% of added cocoa butter.

Key Points

• Dogs can be fed a wide variety of chocolate and cocoa-based goods, particularly during holiday seasons, such as candy, cakes, cookies, brownies, and chocolate chips.

• Dogs that eat chocolate usually become very sick, but they also seldom die from it.

Introduction

Caffeine and theobromine are found in chocolate. Dogs' metabolisms of caffeine and theobromine differ from humans'. Because they contain more theobromine and caffeine, darker, bitter chocolates are considered to be more dangerous.

When a dog consumes too much chocolate goods, it can lead to major health problems like seizures, heart failure, and muscular tremors. In extreme situations, the dog may even pass away from cardiac or respiratory failure. Due to the fat and sugar content, it induces vomiting and diarrhoea even in non-toxic doses. Numerous substances found in chocolate are hazardous to animals. Although chocolate poisoning can affect a wide variety of animal species, including dogs, cats, horses, and rodents, it most frequently affects dogs. Theobromine and caffeine are the main harmful ingredients in chocolate. The amount of caffeine and theobromine in chocolate varies with kind. Dogs' metabolism of theobromine and caffeine differs from that of humans. Dogs are more sensitive because of this. Dogs who consume chocolate may become seriously unwell, even though it rarely results in death. When a dog eats too many chocolate-containing foods, such as cakes, cookies, brownies, candies, and chocolate chips, it might become poisoned.

Many crops can be grown in greenhouse but the choice depends on number of factors.

1.Requirement of the farmer, what for she/he wants to grow the crop.

2.For personal use number of vegetable crops and herbs and fruits like strawberry and papaya can be grown.

3.In case of commercial crops, the market must be ascertained before the choice of the crop.

4.Greenhouse must be equipped to create micro-climate for the crop of choice.

5.The choice of crop is a crucial factor for the economic feasibility and success of a commercial greenhouse operation. The revenue of cultivated crop makes the venture to progress.

6.Crop varieties, suitable for greenhouse cultivation should differentiate themselves from open-field varieties to fetch better price on quality.