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Indian Government has several developmental programmes to promote agriculture and rural sector. It includes Drought Prone Area Program, Antodaya Programme, Integrated Rural Development Programme, Hill Area Development Programme, Desert Development Programme, Intensive Jawahar Rojgar Yojna and Employment Assurance Scheme, Indira Awaas Yojna, Integrated Waste Land Development Programme, National Food Security Mission, National Horticulture Mission, etc. Mahatma Gandhi National Rural Employment Guarantee Scheme (MGNREGS) has the main focus to provide guaranteed employment for 100 days in a year to every rural household. Since 2014, the Swachh Bharat Abhiyan has been a major initiative by the Government of India.
THE FOOD SECUR3ITY BILL
The Food Security Bill is here. Some of the salient features are
Nearly 82 crore (820 m) poor people to get food grains at highly subsidized rate, nearly 20 crore poor in urban areas and 62 crore in rural areas will be eligible to get 5 kg rice, wheat and coarse grains at Rs.3, 2 and 1 respectively. Additionally for pregnant women, there will be a provision for free food for 10 months and for children below 7 years, food will be free. There is one danger point feared in this scheme that in the long run, many farmers will stop farming and go for easier way for casual labour and take food security cover in stead of farming the low production lands. But on the other hand we should look at the solution to this keeping in mind that the Food Security Bill is here and how best we can build on our strength rather than weakness. The areas of strength to look for should be :

Indian Government has several developmental programmes to promote agriculture and rural sector. It includes Drought Prone Area Program, Antodaya Programme, Integrated Rural Development Programme, Hill Area Development Programme, Desert Development Programme, Intensive Jawahar Rojgar Yojna and Employment Assurance Scheme, Indira AwaasYojna, Integrated Waste Land Development Programme, National Food Security Mission, National Horticulture Mission, etc. Mahatma Gandhi National Rural Employment Guarantee Scheme (MGNREGS) has the main focus to provide guaranteed employment for 100 days in a year to every rural household. Since 2014, the Swachh Bharat Abhiyan has been a major initiative by the Government of India.

One ofthe biggest issues the world is facing today is the lack of clean and pure water. Due to rising water demands, rigid health regulations, and xenobiotic pollutants, traditional wastewater treatment technologies cannot provide safe water. The present era necessitates innovative approaches to wastewater treatment technologies and improved water resource conservation and management materials. By utilizing innovative nanomaterial, nanotechnology guarantees a continuous provision of potable water and contributes to the preservation of water sources. Nanomaterials expedite rapid processes for the purification, conservation, and reuse of wastewater, complementing other enduring industrial methods. 

1. Introduction

Cleaning and grading are the first and vital post harvest operations undertaken to remove foreign and undesirable materials such as crop seed, chaff, mud, stones, straws, immature grains, dust, husk and even iron and steel particles from grains as well as fruits and vegetables and to separate the products into various fractions. Generally, the hand threshing and the traditional handling used in most developing countries cause a larger percentage of foreign matter with these crops. Thus, more cleaning is required. Cleaning is essentially a separation process, in which some difference in physical properties of the contaminants and the food units is exploited. 

Fruits and vegetables are more perishable and generally have a higher value compared to cereals, making them very important in terms of minimizing waste and improving employability in the food industry. The losses in fruits and vegetables are significant, but through effective post-harvest processing, these losses can be reduced, and surplus produce can be better utilized. This processing involves a variety of techniques that prepare fruits and vegetables for conversion into processed foods. The food preservation and processing industry has evolved from being a luxury to an essential sector, playing a crucial role in conserving and enhancing the utilization of fruits and vegetables. To prevent waste during peak seasons and ensure availability during off-seasons, modern methods of storage and processing are necessary. These techniques allow for both large-scale and small-scale processing, extending the shelf life of produce for better distribution. The ultimate goal of food processing is to apply systematic techniques to preserve, process, package, store, and distribute food in a way that ensures a steady supply of diverse and nutritious food options, thereby improving food intake and nutritional standards, particularly during periods of low availability.
For effective preservation and processing, key prerequisites include cleaning, grading, and sorting the produce based on its maturity level. This ensures that only the highest quality fruits and vegetables are used in the processing chain, maintaining food safety, nutritional value, and consumer acceptability throughout the year.
CLEANING OF AGRICULTURAL COMMODITIES
Blanching is a unit operation used in food processing to remove contaminating materials from food and prepare the surface for further processing. In vegetable processing, blanching serves the additional purpose of cleaning the product. This process involves briefly exposing the food to heat, typically by immersing it in hot water or steam, which helps to remove dirt, enzymes, and microbial contamination. By doing so, blanching not only improves the quality of the food but also prevents microbial growth during storage or delays in processing. This early removal of contaminants ensures that the remaining bulk of food is preserved and remains safe for consumption throughout its shelf life.

29.1 Introduction
Cleaning is achieved using a cleaning agent designed to remove food, dirt, rust stains, minerals, or other residues. It is crucial to choose the appropriate cleaning agent, as not all are suitable for surfaces that come into contact with food. In a food-processing facility, sanitation involves ensuring that utensils and equipment that come into contact with products are kept clean and sterile. Additionally, clothing, air, packaging materials, and non-contact surfaces like floors, walls, and ceilings must be sanitized to prevent product contamination. Employees’ bodies, especially their hands, can also be sources of contamination, so maintaining high personal hygiene and cleanliness standards is essential. Moisture and heat can lead to microbiological contamination of both products and the environment. Moisture on product-contact surfaces and within products creates a medium where microorganisms can thrive and find nutrients, potentially affecting the safety and quality of milk and dairy products.
29.2 Types of Soil
Cleaning involves removing food residues and other types of soil from surfaces like utensils and equipment used in the dairy and food industries.
• In the dairy-processing sector, the equipment must be cleaned of various types of soil including: liquid milk films, air-dried films, heatprecipitated films, heat-hardened films, milk stone, and other foreign materials.
• Soluble soils will dissolve and be carried away in rinse or wash water. For insoluble soil components, chemical detergents may be needed to either dissolve them or soften them to facilitate easier removal.
• Cleaning or sanitizing the product contact surfaces of dairy equipment involves both washing and sterilizing. It’s important to avoid causing undue wear, erosion, or corrosion to these surfaces, as this can reduce their lifespan

There is no single factor as important in the production of fine quality dairy products as absolute and complete cleanliness.

R.B. Barrett

It is not enough to provide milk for man, it must be high quality milk. It would be impossible to provide man with milk and milk products of superior quality if we were unable to remove milk soil and to kill residual microorganisms. Respectively, these are the processes of cleaning and sanitizing.

To successfully provide the atmosphere for proper food safety, the basics must be thoroughly understood. The basics of food safety begin with good understanding of sanitation processes, food-handling procedures that protect from, or eliminate cross contamination problems and specific attention to inspection of and follow up for all processes. Food safety activities need to be programmed into available time of day scheduling just as production requirements do. Whether special personnel or separate shifts handle cleaning responsibilities or you clean as you go on the production line, time must be made available. Safe products leaving the facility must be the watch word of all employees involved with the process.

In order to ensure safe food supply a high level of sanitation should be maintained throughout the food production chain. The word ‘sanitation’ is derived from the Latin word “sanitas” which means “health”. To further apply this word to the food industry, sanitation is the creation and maintenance of hygienic and healthful conditions when processing, preparing and handling food. Sanitation is the application of science to provide wholesome food handled in a hygienic environment by healthy food handlers to prevent contamination by food spoilage microorganisms. Effective sanitation refers to the mechanisms, which accomplish these goals.

Cleaning and sanitisation are the two processes employed in dairy industry to ensure hygiene and product safety. The safety of milk and milk products is a very important aspect and every attempt should be made, during processing, to minimize contamination through handling and processing equipments. The accumulated dried milk solids (milk stones) consist largely of calcium phosphate, milk proteins (precipitated, coagulated and baked on by heat) and insoluble calcium salts from water and washing detergents. The surface can also contain biofilms, which are formed by the incorporation of bacteria into the accumulated inorganic and organic materials that attach to most surfaces. With time and in the presence of nutrients, a biofilm and the bacteria within the biofilm will grow and become strongly attached to the surface. The removal of dairy soil, milk stones as well biofilms is the prime objective of the cleaning process. International Dairy Federation (IDF) defines a clean surface as one which is free from residual film or soil that will not contaminate the food products coming in contact with it.

While cleaning refers to the removal of soil from the surface of the equipment, sanitisation implies destruction of all pathogenic and almost all non-pathogenic organisms. Cleaning and sanitisation are complimentary processes. Either of the processes alone will not achieve the desired result, which is to leave the surface as free as possible from soil and viable organisms. In actual practice cleaning and sanitisation overlap to some extent, as during cleaning a high proportion of bacteria are destroyed and others get removed in the detergent solution. Inadequate or faulty cleaning and sanitisation can have serious consequences, as milk is a prefect nutrient in which bacteria can multiply. A thorough understanding of the cleaning methods is, therefore, necessary for economic production of high quality food products.

Cleaning and sanitizing of dairy plants is essential for providing safe milk products to the consumers as well as for ensuring the good shelf life of these products. It ensures cleanliness, hygiene of the equipments and surroundings and also ensures that the products meet the safety standards. Cleaning of dairy plant consists of broadly of removing the soil from the surface of the equipment by dissolving or suspending the soil in a warm solution of suitable cleaning agents. In the dairy industry, the soil is primarily milk and milk product residues which may be more or less modified by processing treatment. 
Milk and milk product residues are complex mixtures of milk sugar, fat, proteins and mineral salts. Some of these solids are present in emulsion, some are in a colloidal state, and others are in true solution. When milk products are heated in vats or pumped through sanitary pipe lines or over coolers, the metal or glass surfaces become coated with milk remains, which, if allowed to dry, may be difficult to remove.

Cleaning and sanitation are essential for maintaining microbiological safety in ice cream manufacturing, particularly because contamination can occur after pasteurization and freezing does not eliminate all microorganisms. This chapter explains contamination routes, hygienic zoning, cleaning systems, sanitization practices, HACCP principles, and microbiological expectations within an ice cream plant. It examines CIP and COP systems, detergent components, cleaning factors, high-risk equipment and surfaces, and post-pasteurization contamination control. Particular emphasis is placed on preventing biofilms and controlling contamination at filling equipment, tanks, pipelines, feeders, drains, packaging areas, and personnel interfaces. The chapter provides practical sanitation guidance to support food safety, product stability, regulatory compliance, and consumer protection

Immediately after use, all sorts of items are thoroughly washed with water and the rubber catheters are washed with the force of tap water to remove the residual diluted semen. The glass wares are kept in 1% chromic acid solution for overnight to remove cloudiness of glass surface. Then the articles are washed with lukewarm laboratory grade detergent (ex. Labolene®) with a brush to make the articles grease free. All the items are thoroughly washed several times under distilled water and kept inverted position in a clean trough then the openings of glassware wrapped with aluminum foil / paper.

The equipments used in artificial insemination should be clean and sterilized. The use of unsterilized equipments in AI causes the spread of infection in the herd and infertility. Rinse the equipments with tap water immediately after use and then lukewarm detergent solution. The detergent used for washing the AI equipments should be non corrosive. Use of corrosive detergents will reduce half-life of the plastic and rubber wares. Wash thoroughly with tap water till the equipments become grease free and perfectly clean. Then rinse in distilled water at least thrice. Use of distilled water does not leave any salt residues on articles after drying.

1. Introduction

To maintain good quality of product & shelf life, it is very important & essential to concentrate on Cleaning & Sanitization process at plant level & to follow the CIP process properly. An attempt is made in the current chapter to present details pertaining to cleaning in fluid milk and milk products manufacturing & processing plants. This chapter will be helpful the students of Food & Dairy Technology for better understanding the process milk plant cleaning & sanitation technology.

Cleaning, drying, and aeration are essential processes in the management of stored agricultural products to maintain their quality and reduce the risk of spoilage. These practices are particularly important in preventing the growth of molds, the infestation of pests, and the deterioration of the product during storage.
Cleaning is the first step in the storage process. It involves removing any debris, dust, dirt, and residues from both the stored products and storage facilities. Proper cleaning eliminates potential sources of contamination, such as pests, molds, and bacteria, which can thrive in the presence of organic matter. It also ensures that the grain or other stored product is free from contaminants that could affect its quality and marketability.
Drying is equally crucial to prevent moisture-related issues. Excess moisture can lead to mold growth, fungal infections, and insect infestations. Drying reduces the moisture content of the grain or product to safe levels, typically below 14% for grains, which helps preserve their quality during storage.
The drying process can be carried out using natural sunlight or mechanical dryers, depending on the volume of the product and available resources. Properly dried products are less prone to spoilage and can be stored for longer periods. Aeration involves the circulation of air through stored products to maintain optimal temperature and humidity levels. By controlling the environment, aeration helps prevent the buildup of heat and moisture, both of which can lead to product deterioration.
It also reduces the risk of insect infestations by keeping the grain cool and dry. Aeration systems typically use fans or ventilation channels to ensure continuous airflow, especially in large-scale storage facilities. Together, these processes form a comprehensive approach to maintaining the quality and safety of stored agricultural products.

Introduction
• The source of contamination of meat plant is largely from the animals entering in the plant
• Severely contaminated hide/skin are the major source of abattoir contamination
• If proper hygiene of employees, equipments and clothing is not followed, then even after skinning 10,000 to 100,000 bacteria/ in2 can be detected
• Blades of knives can carry 80,000 to 40 millions bacterial per blade & hands of meat operative can have 3x109 bacteria/g of scrapings
• The chance of contamination of carcase is greater at the point of incision and minimum at the farthest point from the incision place
‘Scotoma effect’ or ‘factory-blindness’
• Persons working in any factory or abattoir on routine basis slowly and slowly becomes unaware (mentally ‘blind’) about any sub-standard hygiene or cleanliness problem existing in the surrounding
• This effect is known as ‘scotoma’ or ‘factory-blindness’
• Effect can be powerful and the any visitors to that factory or abattoir will instantly notice that sub-standard hygiene or cleanliness problem neglected by the persons working there

10.1 Introduction

Food safety and quality are the two important factor which results in consumer food choices. Nowadays consumers are demanding food which is safe and of high quality in terms of nutrition due to a healthier life style. Food safety and quality are the terms used interchangeably. Food safety refers to all those hazards, that make food injurious to the health of the consumer and it is not negotiable. Food quality includes attributes that influences the product's value to the consumer. This includes both negative and positive attributes; negative attributes includes spoilage, contamination, discoloration, off-odours and positive attributes include colour, flavour, texture and processing method of the food. This distinction between safety and quality has implications for public policy and influences the nature of the food control system. Food control is defined as a mandatory regulatory activity which provides consumer protection and ensures food is safe, wholesome and fit for human consumption during production, handling, storage, processing, and distribution.

Despite the huge efforts made by the food safety authorities and industries, food safety still remains a critical issue. In food industries, producers have to satisfy both safety and quality criteria for their products to the consumer. Food processors have multiple options in terms of different quality and management systems, they have to choose the most appropriate one for its specific activity and should establish, document and implement effective systems in the processing area for managing quality and safety of the product. Over the past few decades, food-borne illnesses have raised great concern about the safety of food. Considerable steps are taken by the food processor for understanding and managing the risks of airborne diseases. Among various systems, Hazard Analysis and Critical Control Point (HACCP) is effective tool which focuses mainly on controlling hazards to ensure the production of safe and wholesome food. For maintaining the safety and increasing the shelf life of food, traditionally it has been preserved by thermal processing, freezing, salting and drying. But nowadays consumers are demanding convenient, innovative, fresh foods, including new "minimally processed" products. To meet consumers' expectations in the 21^st century, the food industries are implementing novel technologies whose purpose will be two fold: 1) to provide the new quality attributes demanded by consumers; and 2) to ensure the all important and often expected assurance of food safety. The novel technologies will include various minimally processed technologies like high pressure processing (HPP), pulsed electric field (PEF), ohmic heating, dielectric heating, microwave heating, ultrasound, modified atmosphere packaging (MAP) and cleanroom technology. Among these cleanroom technology have attracted great attention for maintaining the safety and quality of the food products as because it doesn't have any effect on the nutritional quality of the food being processed, it maintains the hygienic condition in the processing area, remove contaminants from the processing area and clean air in the processing area which in turn results in safe food product. The main objective of cleanroom technology is to eliminate airborne contaminants from the humans and/or from the environment. An air curtains and a positive air pressure is maintained in the processing unit using filtered air. Production personnel will use the special clothing which is preferred for cleanroom to reduce the contamination from both air- and human-borne microorganisms. In this chapter we will discuss about cleanroom technology, its historical background, its classification, layout of cleanroom, air handling, personnel and its clothing, regulations in cleanroom, and its application in food industries.

Introduction
Cleavage is the process of cellular division without growth. It is also called segmentation division and it follows immediately after fertilization. By the end of this process, single cell zygote is transformed into multi-cellular stage called blastula. Daughter cells are called blastomeres. All divisions are mitotic and occur consequently. Ratio of nucleus to cytoplasm is very low at beginning but later they resemble somatic cell. Cleavage division always start at animal pole. Cleavage can be classified as:
1. Holoblastic/Total cleavage: Whole fertilized ovum is involved in production of blastomere. It is also called complete cleavage.
a) Equal: All blastomeres are of equal size. E.g. mammals
b) Unequal: Some blastomeres at vegetal pole are larger. E.g. amphibians
2. Meroblastic/Partial cleavage: When deutoplasm is abundant and only a part of fertilized ovum is involved in cleavage, it is called meroblastic cleavage.
a) Discoidal: Cleavage is confined to the germinal disc i.e. at animal pole. E.g. birds and reptiles.
b) Superficial: Cleavage is confined to the peripheral margin of cytoplasm. E.g. arthropods.
Dividing continuously, embryo attains a stage of 16-32 cells. 16-celled stage is called early/uncompact morula and 32-celled stage is called compact morula. This stage is called morula due to its close resemblance to mulberry fruit. Structure is surrounded by zona pellucida.
After morula stage, fluid accumulation begins and blastomeres are gradually pushed to the periphery. As fluid accumulation progresses further, it leads to formation of large cavity in central part of embryo known as blastocoels and morula stage is now transformed into blastula/blastocyst stage. Blastomeres now arrange themselves as single celled outer layer called trophoblast and small group of cells at animal pole called inner cell mass (ICM). Trophoblasts give rise to fetal membranes and will help embryo in its attachment to uterine wall (implantation). ICM gives rise to all organs of body. Development of blastula is called blastulation.

Synonym : PCR-RFLP (PCR- Restriction Fragment Length Polymorphism)

The Cleaved Amplified Polymorphic Sequence or CAPS method is a technique in molecular biology for the analysis of genetic markers. It is an extension to the Restriction Fragment Length Polymorphism (RFLP) method, using polymerase chain reaction (PCR) to more quickly analyse the results.

Like RFLP, CAPS works on the principle that genetic differences between individuals can create or abolish restriction endonuclease restriction sites, and that these differences can be detected in the resulting DNA fragment length after digestion.

In the CAPS method, PCR amplification is directed across the altered restriction site, and the products digested with the restriction enzyme. When fractionated by agarose or acrylamide gel electrophoresis, the digested PCR products will give readily distinguishable patterns of bands. Alternatively, the amplified segment can by analyzed by Allele specific oligonucleotide (ASO) probes, a process that can often be done by a simple Dot blot.

Clematis is member of the Ranunculaceae family which also known as buttercup family. This cosmopolitan genus comprises climbing lianas, small shrubs and erect sub-shrubs distributed predominantly in the temperate zone of both hemispheres but with some species distributed in tropical areas (Tamura, 1995, Johnson, 1997, Grey-Wilson, 2000 and Wang and Li, 2005). The word clematis is taken from Greek as in “klema” meaning “a vine branch”. Most species are known as Clematis in English while some are also known as Traveller’s joy, Leather flower, Vase vine and Virgin’s bower the last three being names used for North American species (Chawla et al., 2012). Most Clematis is climbers (Roh and Song, 1997), however; two notable exceptions are the species C. heracleifolia and C. integrifolia which are herbaceous and fit well in a perennial garden border. The vast majority of these are very hardy. Some species however, particularly the evergreens can only take a few degrees of frost. Most Clematis varieties produce single flowers. Some varieties produce double flowers; others produce both single and double flowers. Clematis blossoms often change colour, some very marked through the life of each flower particularly when grown in full sun. Pastel colours will hold their colour best if grown in some shade. Many Clematis species are scented, although none are strongly perfumed. The most-highly scented is the tender species C. armandii. Montana types can also be relatively aromatic often with a scent reminiscent of vanilla. Of the large flowering hybrids, Fair Rosamund is the only one with any noticeable scent.

Clematis is member of the Ranunculaceae family which also known as buttercup family. This cosmopolitan genus comprises climbing lianas, small shrubs and erect sub-shrubs distributed predominantly in the temperate zone of both hemispheres but with some species distributed in tropical areas (Tamura, 1995, Johnson, 1997, Grey-Wilson, 2000 and Wang and Li, 2005). The word clematis is taken from Greek as in “klema” meaning “a vine branch”. Most species are known as Clematis in English while some are also known as Traveller’s joy, Leather flower, Vase vine and Virgin’s bower the last three being names used for North American species (Chawla et al., 2012).