eChapters

Sheep and goat rearing in India continues to be traditional even today. This sector is primarily in the hands of poor, landless or small and marginal farmers who raise their animals on natural vegetation, stubbles and supplement tree lopping. The productivity of sheep in India is relatively low with an average body weight gain of 20 to 50 g/day. The major causes for the low productivity are inadequate grazing resources, tropical heat and disease incidences and a lack of awareness about improved management practices.

Pig production is concentrated mainly in the North-eastern corner of the country and consists primarily of backyard and informal sector producers. Though pig population is only 2% of all the livestock animals, Pork production in India  represents 7% of the country’s total meat production. This value is highest compared to any other species of meat animals.

1 Broilers

The broilers are also called young meat chickens; generally attend a body weight of 2 kg at 35-38 days of age with a feed conversion of 1.5-1.8. This improvement in growth is equivalent to reduction of market age by one day per year. This fast growth is possible due to intense feeding good quality feed & growth promoters in less space. However, this type of rearing has challenges many welfare issues in broiler farming.

Good livestock welfare practices demands thorough understanding of the basic nature of the animal. The science of understanding the details of animal behaviour is known as Ethology.  Knowledge about the behaviour of the species being reared proved as an important tool for implementing animal welfare in practical husbandry practices. Now a day’s global community is also advocating for the better livestock welfare standards. Exploiting animal’s natural behaviour could certainly prevent unnecessary sufferings related to handling and restraining during routine farm activities. On the one hand this ensures physical and mental welfare of livestock, while on the other hand improvement in the production is guaranteed. Without involving heavy investment behavioural knowledge based management interventions can bring paradigm shift in welfare status of farm. Therefore, behavioural studies are of great importance in increasing our understanding and appreciation of animals.

India is known for its rich wildlife heritage. In fact, the country is considered one of the best among available mega-biodiversity areas in the world. The landscape and agro-climatic conditions of India make it a unique habitat for wild animals. The government of India has made attempts to conserve wildlife through different projects like Corbett tiger, Elephant projects etc. The wild life management in India is at present ‘preservation-oriented’. This is bound to remain so for a long time because of the significantly depleted status of our wild life population. Indian government in pursuit of conserving the heritage of wilderness has made committed efforts with several wildlife and Forest Acts to ensure protection by declaring the areas as protected under the spectrum of national park and wildlife sanctuaries. The eco-tourism and conservation awareness has created environmental consciousness among the people.

The wildlife (protection) Act, 1972 (No. 53 of 1972) consists of 7 chapters, 6 schedules and 66 sections. This act has been amended in 1980. This act further amended as The Wildlife (Protection) Amendment Act- 2002, from 17thJanuary, 2003. This act deals with all those matters related to wildlife in captivity/free range for conservation.

Chapter I (Preliminary)

It discusses about the terminologies and definition
Captive animal means any animal, specified in Schedule 1, Schedule II, Schedule III or Schedule IV, which is captured or kept or bred in captivity.
Circus means an establishment, whether stationary or mobile where animals are kept or used wholly or mainly for the purpose of performing tricks or manoeuvrers.

All farmed animals are transported at some stage in their lives, sometimes to places where food is more readily available, sometimes to a different owner or a different place of keeping and sometimes to slaughter. The handling, loading, transporting and unloading of animals can have very substantial effects on their welfare. Wherever   poor welfare associated with the transport of animals is prevented, there is an immediate financial advantage because mortality rates and carcass downgrading are reduced. The attitude towards animals has major consequences on animal welfare during transportation. Therefore, laws and codes of practice can also have significant effects on animal welfare during transport. In order to produce guidelines that can be used to prevent or minimise poor welfare in animals during transport, it is necessary to be aware of the biological functioning of the animals concerned and the attitudes and actions of the people involved in the handling and transport procedures.

In India, the cow is a worshipped as symbol of wealth, strength, abundance, altruistic giving and full of earthly life. Cows are traditionally treated as a sacred animal. Dairy products are extensively used in India and are one of the most essential nutritional components of meals.
 
The laws governing cattle slaughter is shown in entry 15 of the State List of the Seventh Schedule of the Constitution, meaning that State legislatures have exclusive powers to legislate the prevention of slaughter and preservation of cattle. Some States allow the slaughter of cattle with restrictions like a “fit-for-slaughter” certificate which may be issued depending on factors like age and gender of cattle, continued economic viability etc. Others completely ban cattle slaughter, while there is no restriction in a few states. Prohibition of cow slaughter is a Directive Principles of State Policy contained in Article 48 of the Constitution.

A disaster (from Latin meaning, “bad star’) is the impact of a natural or man-made event that negatively affects life, property, livelihood or industry often resulting in permanent changes to human societies, ecosystems and environment. The event itself is not a disaster; it is the impact that is a disaster. Their possibility of occurrence, time, place and severity of the strike can be reasonably and in some cases accurately predicted by technological and scientific advances. Hence, we can to some extent reduce the impact of damage though we cannot reduce the extent of damage itself.  The impacts suffered include trauma, starvation, dehydration, infection, disease, shock etc. Animals are often the silent victims of disasters, and there is a real need around for the provision of effective training and management programmes to ensure that their survival and welfare are adequately accounted for. This demands the study of disaster management in methodical and orderly approach.

Various indicators like range of behavioural, physiological, pathological and carcass-quality parameters can be used to assess the welfare of animals. Whenever, animals undergo painful handling or any other managerial operation, they are likely to face short-term stress. In this condition an increased physiological responses, behavioural responses, injury or mortality are most commonly used; however, some animals which are kept in improper housing with less floor space may cause chronic stress leading to increased disease incidence and suppression of normal development. Therefore, the following techniques can be used to assess the animal welfare at farm level in any given situation.

Animal’s response  to external stimuli is a behavior. Domestic animal behaviour and performance are correlated. Man’s intervention has led to huge modifications and modulations in animal behaviour patterns over ages but certain characters and normal behaviours of animals are needed to be expressed, otherwise may lead to abnormalities or performance loss. Animal freedoms also ensure certain basic freedoms to domesticated animals including freedom to express normal behaviour. With the developing concern over animal welfare among consumers, growers and animal activists, the need to ensure animal freedoms is call of the hour. Measuring animal welfare is a complex process which requires multidisciplinary approaches. There are four approaches to determine animal welfare viz, productivity, animal health and disease, physiology and behavior which are used individually or in combination.

Use of animals in teaching is an inevitable process of learning in Veterinary and Animal Science colleges. Consequently, animals have to be handled for anatomical demonstration, surgical practice and used as experimental animals in various investigatory researches. The apprehensions and pain the animals have to suffer from these handlings (mishandlings) have brought the need to look for alternative ways to deal with animals and yet the purpose of learning about them does not diminish. Now-a-days, a growing emphasis is placed on minimizing the overall use of animals alongside advancements in science.

1.1 Introduction

With the rise in the global population, the demand for increased supply of food has motivated scientists and engineers to design new methods to boost agricultural production. With limited availability of land and water resources, growth in agriculture can be achieved only by increasing productivity through good agronomy and supported with an effective use of modern technology. Advanced agronomical methods lay stress not only on boosting agricultural produce through use of more effective fertilizers and pesticides, but also on the hygienic storage of agricultural produce. The detrimental effects of modern agricultural methods on the ecosystem have raised serious concerns amongst environmentalists. The widespread use of persistent pesticides globally over the last six decades has contaminated groundwater and soil, resulting in diseases and hardships in non-target species such as humans and animals. The first step in the removal of disease causing microbes from food products or harmful contaminants from soil and groundwater is the effective detection of these damaging elements. Nanotechnology can augment agricultural production and boost food processing industry through applications of these unique properties.

2.1 Introduction

The word Nanotechnology has originated from a Greek word which means “dwarf” and nanometer is one billionth of a meter (1 nm = 10^-9 meter). The nano-scale is not just another step toward miniaturization but a qualitatively new scale. The new behiviour of matter at nano-scale is dominated by quantum mechanics, material confinement, large interfacial volume fraction and other unique properties, phenomenon and processes. At nano-scale, physics, chemistry and biology converge towards the same principles and tools. Nanotechnology cannot be considered as a discipline in science or technology but it is domain in science and technology. Nanotechnology is a multidisciplinary area, any scientist, engineer, medical doctor can do research on nanotechnology. Presently, it is more of a science and less of a technology because of a lack of proper tools for studying the properties of nano-material. There is an immediate need to convert this science into technology and get out of this transition stage.

3.1 Introduction

The concept of nanoscience was first described in 1959 by physicist Richard P. Feynman in a lecture to the American Physical Society and it was Japanese researcher Norio Taniguchi who coined the term nanotechnology in 1974 to describe precision engineering with tolerances of a micron or less. Eric Drexler in 1986 in his book Engines of Creation, brought nanotechnology into the public domain.

4.1 Introduction

More and more industrial applications require self cleaned surfaces, water repellent, anti-dirt pickup and many other specific surface requirements. Introduction of nanotechnology has changed the coating scenario drastically. Hard, anti-scratch, hydrophobic coatings are achieved by the addition of small amounts of nano-pigments which help in creating hydrophobic surface. It is also possible to grow certain nano-fibers which on chemical treatment give surfaces which can either attract water or repel it. It is also possible now to create self healing coatings which can repair surface once they are damaged by scratch. Capacitance coupler pads have been devised to reach those corroding areas which are not covered by other corrosion protection methods. Stringent requirements of many modern industrial application resulted in developing coatings which can give special surface properties such as high hardness, scratch resistance, hydrophobicity, anti-dirt pickup etc.  This brief article presents a few new researches which provide one or more special properties to the surface.

5.1 Introduction

Phosphorus fertilization is a problem in acid soils due to the high fixation characteristics of these soils. Soluble phosphorus carriers such as triple super phosphate tend to get reverted into insoluble and slowly soluble forms, for e.g. iron and aluminium phosphates.  Some soils in Mauritius have been shown to fix up to 90% of applied phosphorus. The fixation characteristics as related to soil properties have been studied for some soils of Mauritius. Rock phosphate offers a good alternative or complement to triple super phosphate.  In volcanic soils of Chile it is recommended to apply rock phosphate in combination with triple super phosphate because of the high phosphorus-retention capacity of these soils.  However, in Western Australia it has been shown that rock phosphate response is low. Possible explanations are slow dissolution of rock phosphate due to the moderately acid pH (5.5 -6.5), low pH-buffering capacity, low calcium absorption in soil, which increases concentration of calcium in soil solution, and poor water holding capacity of these soils.

6.1 Introduction

Nanotechnology is the study of manipulating matter on an atomic or a molecular scale that deals with particle sizes between 1 and 100 nano meters at least in one dimension. Particles reduced to the nanoscale show some unusual properties which are different from what they exhibit on a macro scale, enabling unique systematic applications Tarafdar et al. (2012). The unexpected properties of nanoparticles are broadly due to the large surface area of the material, which dominates the contributions made by the very small quantities of the material. Nanoparticles, thus, take advantage of their dramatically increased surface area to volume ratio.

Nanotechnology has the potential to revolutionize the agricultural and food industry with new tools for the molecular treatment of diseases, rapid disease detection, nanofertilizer, enhancing the ability of plants to absorb nutrients etc. Nanotechnology opened doors to new ways of identifying and quantifying biomolecules through use of nanosensors and nanoprobes.

7.1 Introduction

Organic polymers can play important role in ecosystems by accumulating biologically important elements and also by retaining soil moisture after aggregating soil particles. Extracellular polymeric substances (EPS) play an important role in cell aggregation, cell adhesion, and biofilm formation that subsequently protect cells from a hostile environment. Furthermore, certain polysaccharides from microbial sources are surface active, and thus attempts have been made to use them as metal chelaters, emulsifiers and flocculants in industrial and environmental fields/domain. Such use of microbial polysaccharides has infused renewed interest in its production and characteristics.

8.1 Introduction

Nanotechnology is the manoeuvring or self-assembly of individual atoms, molecules, or molecular clusters into structures to craft materials with new properties. The definition of nanotechnology is based on the prefix “nano” which is from the Greek word meaning “dwarf”. In more technical terms, the word “nano” means 10^-9, or one billionth of something. The word nanotechnology is generally used when referring to materials with the size of 1 to 100 nanometres, which is true for metallic nano-material. In case of polymeric nano-material, size may reach 600 nm, as polymeric materials are larger in size compression to metallic material. Nano-materials have different properties from bulk materials as a result of their size. These differences include physical strength, chemical reactivity, electrical conductance, magnetism, and optical effects (Schmid, 2006).

9.1 Introduction

Nanoparticles (NPs) are particulate matter with at least one dimension less than 100 nm (Christian et al., 2008). Although NPs are naturally present in the environment, manufactured NPs may have distinctive surface properties and chemistry in comparison with natural NPs (Handy et al., 2008). Manufactured NPs have a large area-to-volume ratio and size-dependent properties, which impart novel properties and behaviors that make them suitable for nanotechnology applications (Auffan et al., 2009). Range of applications for NPs has brought about a rapid development of the nanotechnology industry.

10.1 Introduction

A single-walled carbon nanotube (SW-CNT) is a nano scale tube formed by a cylindrical shell of single atomic layer of carbon atoms. Nanotubes have diameter of a few nm and length up to 100 nm so that they form extremely thin wires. The atomic structure of SWCNT can be formed by wrapping a stripe of single atomic layer of graphite sheet al.,ong a certain direction, and this direction determines the diameter and chirality of the nanotubes. Experimental and theoretical studies have found that these nano-meter sized CNTs have novel electronic properties, which can be metallic or semiconducting, depending on their radius or chiralities (Mintmire 1992, Hamada 1992, Saito et. al., 1992, Rao 1997, Wildoer 1998 and Odom et al., 1998).

11.1 Introduction

Nanotechnology is the manipulation or self-assembly of individual atoms, molecules, or molecular clusters into structures to create materials and devices with new or vastly different properties. Nanotechnology can work from the top down (which means reducing the size of the smallest structures to the nanoscale e.g. photonics applications in nanoelectronics and nanoengineering) or the bottom up (which involves manipulating individual atoms and molecules into nanostructures and more closely resembles chemistry or biology). The definition of nanotechnology is based on the prefix “nano” which is from the Greek word meaning “dwarf”. In more technical terms, the word “nano” means 10-9, or one billionth of something. It is a very promising new branch of small-scale technology named for the unit of measure at which it operates: the nanometer. Nanotechnology enables us to create functional materials, devices, and systems by controlling matter at the atomic and molecular scales, and to exploit novel properties and phenomena. Consider that most chemical and biological sensors, as well as many physical sensors, depend on interactions occurring at these levels and you’ll get an idea of the effect nanotechnology will have on the sensor world.

12.1 Introduction

“Precision farming,” also known as site-specific management, describes a bundle of new information technologies applied to the management of large-scale, commercial agriculture. Precision farming technologies include, for example: personal computers, satellite-positioning systems, geographic information systems, automated machine guidance, remote sensing devices and telecommunications. If they function as designed, ubiquitous wireless sensors will become an essential tool for bringing this vision of precision farming to maturity.

13.1 Introduction and Physical Principles

A microscope is a tool that enables the user to see objects at a magnification greater than the actual specimen. Resolution and magnifying power are the two integral aspects of microscopy. Resolution is a measure of capability of an image forming system to separate images of adjacent objects. The resolving power (8) is numerically defined as d = 0.61l/n sin a, where,  l is the wavelength of the light, n is the index of refraction of the medium between the specimen and lens, a is half the angle of the cone of light from the specimen plane accepted by the front surface of lens and n sin a is the numerical aperture (NA). The NA represents the light gathering power of the lens aperture or in other words, the measure of obliquity of rays which the lens can accept and utlilize and is limited by blurring of image due to spherical aberration of the lens.

14.1 Introduction

The fast growing field of nanotechnology presents great potential to influence various sectors in the areas of energy, environment, agriculture, health care and consumer goods. To overcome the demanding need of nano-based applications, various strategies to synthesize nanoparticles have been attempted. The major focus is to synthesize nanoparticles of desirable properties such as shape, size and compositions. Various strategies have been employed to synthesize nanoparticles. Foremost among these are physical and chemical methods which include sol-gel technique, solvothermal synthesis, chemical reduction, laser ablation and inert gas condensation. But altogether they suffer from process limitations like difficulty in controlling experimental conditions, feeble reproducibility, polydispersity, costly instrumentation and recovery as well as energy intensive and eco-hazardous processes. However, various factors like cost, speed, efficiency, biocompatibility etc. are still not concerned. Thus, finding a balance between scalability, price and applicability is still a true challenge. These limitations can be potentially overcome by using biological methods for nanoparticle synthesis.

15.1 Introduction

It is important that we understand some terms like clay, mineral, clay mineral, activated clay, and nanocomposite clearly. In fact, “clay” is a soil separate consisting of particles of <2 µm (equivalent to <0.002 mm) in equivalent diameter (Brady & Weil, 2002; pages 18-19) in both USDA and International systems. While in nanotechnology, uniqueness of micro- and nana- aggregates is of crucial consideration. (In Chapter 8: The Colloidal Fraction (Brady & Weil, 2002) size dimensions of minerals are given eliminating any chance of confusion.) Now let us look at some definitions provided in the Glossary of the Clay Minerals Society (CMS) Nomenclature Committee – Clay Science Project (www.clay.org; August 2011).

16.1 Introduction

The stabilization of ultra fine as nano-particles in suspension is very important for both controlling the particle size and for developing process based application of these suspensions to achieve a desired result. Generally, two approaches, namely, Top Down and Bottom-Up approaches are followed for the preparation of ultra fine or nano particles. In the Top-Down method, production starts from the micro-scale and the particles size is reduced to nano-size through repeated impact and collision in high-energy ball mills. However, controlling of size, morphology, surface properties and electrostatic charge is often difficult. In the Bottom-up approach, production of nano-particles starts with the individual atoms and building up to a nano-structure by chemical processes.

17.1 Introduction

In industrialized nations the air is filled with numerous pollutants caused by human activity or industrial processes, such as carbon monoxide (CO), chlorofluorocarbons (CFC), heavy metals (arsenic, chromium, lead, cadmium, mercury, zinc), hydrocarbons, nitrogen oxides, organic chemicals (volatile organic compounds, known as VOCs, and dioxins), sulphur and other particulates. The presence of nitrogen and sulphur oxide in the air generates acid rain that infiltrates and contaminates the soil. The high levels of nitrogen and sulphur oxide in the atmosphere are mainly due to human activities, particularly burning of oil, coal and gas. Only a small portion comes from natural processes such as volcanic action and decay of soil bacteria. Water pollution is caused by numerous factors, including sewage, oil spills, leaking of fertilisers, herbicides and pesticides from land, by-products from manufacturing and extracted or burned fossil fuels.

18.1 Introduction

Nano – Science & Technology is design, fabrication and application of nanostructures or nanomaterials, and the fundamental understanding of the relationships between physical properties or phenomena and material dimensions at nano meter scales.

19.1 Introduction

Nano technology or Nano Science is a new branch of research though nano materials or Nano Structures are not new. In nature nano particles or nano materials are abundant .The idea of manipulation, production, controlling size and physico - chemical properties and application as well as making devices out of these nano materials is the subject of Nano Science & Nano Technology. As the name suggests, the domain of Nano starts from 10-9 m but especially the effect of nano structures exists mostly in the 1-100 nm regime where quantum - mechanical behiviour is prevalent. Molecules with more than 10 atoms fall into the nanometer range  and scientists were not able to visualize individual molecules and nano scale objects until the invention of some very powerful microscopes. Working in Nano domain requires two basic facilities -

20.1 Introduction
  
Fertilizers play an important role in improving soil fertility and productivity of crops regardless of nature of cropping sequence or environmental conditions. It has been unequivocally demonstrated that one third of crop productivity is dictated by fertilizers besides influencing use efficiencies of other agri-inputs. In the past four decades, nutrient use efficiency (NUE) of crops had hardly exceeded 35-50%, 18-20% and 30-35 % for N, P and K, respectively, despite our relentless efforts. The remaining nutrients stay in soil or enter into the aquatic environment causing eutrophication. In addition to the low nutrient efficiencies, Indian agriculture is facing a problem of low organic matter, imbalanced fertilization and low fertilizer response that eventually caused crop yield stagnation (Biswas and sharma, 2008).The fertilizer response ratio in the irrigated areas of the country  has dcreased from 13.4 kg grain / kg nutrient applied in 1970’s to just 3.7 kg in 2005. In other words, more amounts of fertilizers is required to produce the same quantity of grain output. For instance, 27 kg NPK ha-1 was required to produce one tonne of grain in 1970 while the same level of production can be achieved by 109 kg NPK ha-1 in 2008. 

21.1 Introduction

Nanotechnology is an emerging field of science which is being highly exploited in the sphere of medicine where “nano” plays a vital role in delivery of drugs or chemical molecules to the point it is required in précised quantities in right proportions. Nanoscience infuses intelligence to the truck load of chemical constituents that are to be delivered at appropriate locations and cleave from the site after the task is complete. Such process will likely to reduce the cost besides ensuring environmental safety. Nanoscale devices with its unique properties make the agricultural system more smart and effective; such devices are capable of responding to different situations by themselves, thus taking appropriate remedial action with the need of external directions from humans. In short, these devices act as a detectors and if need arises serve as a solution/remedy for the particular issue. These smart delivery systems of chemicals in controlled and targeted manner can be considered synonymous to the proposed nano-drug delivery system in human (Patolsky et al., 2006). 

22.1 Introduction

Nanotechnology has proved to have infinite potential in increasing the quality of life of the human population. There is however, a growing realization regarding the potential health and safety risks of associated with development, production and application of these nanoparticles. Nanoscale particles are produced from a variety of different processes. Nanoparticles are the end product of a wide variety of physical, chemical and biological processes, some of which are novel and radically different, others of which are quite commonplace. Nanometer sized particles are also found in the atmosphere where they originate from combustion sources (traffic, forest fires), volcanic activity, and from atmospheric gas to particle conversion processes such as photochemically driven nucleation. A large quantity of nanoscale particles are also produced during the various human activities, Welding can generate large quantities of nanoparticles usually in the form of a well defined plume of aggregated nanoparticles.

A tumour/neoplasm is caused by a purposeless multiplication of living cells. The word neoplasm means new growth. Tumours are common in carnivore as compared to other animals. Horse and cattle are mostly affected than sheep, pig and goats. Old animals are affected more commonly than young ones. Cancer, according to current opinion, is a multitude of different diseases and is no more a “one diseases’. Myriads of tumour types and plethora of their patho-physiology in different species necessitates species wise study of tumours in detail.  Of all species, dog develops cancer twice as frequently as humans. Mammary tumours rank second (behind skin tumours) as the most common neoplasm in dogs and constituting more than 50% tumours in bitches.

Canine mammary gland tumours are the most common type of tumour in the intact female dog. Of all species, dog develops neoplasm twice as frequently as humans, with incidence of skin and mammary tumours being the highest. Tumours of mammary gland are the most common tumours of female dog representing approximately 30-50% of all tumours in bitch and it is the second most common tumour in dog after skin neoplasm and most common malignant tumour in dog.

Defination

Mammary gland tumours arise primarily in the female animal from secretary epithelial, myoepithelial, or, less frequently, mesenchymal elements of mammary tissue.

Cancer remains a challenge because of its incurability and fatality.  Cancer is one of the leading causes of death in companion animals such as dogs and cats and is particularly common in animals that live 10 years or longer. Of all species, dogs develop neoplasm twice as frequently as humans, with incidence of skin and mammary tumours being the highest.

Data on incidence rates are relatively new development in the veterinary medicine. One of the major sources of various dog neoplasm incidence rates has been a population based animal tumour registry. Though, majority of epidemiological studies have been reported from various countries, in India knowledge about epidemiology of canine neoplasm is scant.

The breeds of animals are utilized for this study includes Spitz (12), German shepherd (7), Non descript (5), Doberman (2), Rottweiler (1), Labrador (1) and Pomeranian (2) (Total 30 clinical cases of CMT). The animals are weighed between seven to thirty nine kilograms. There are twenty three cases of non spayed and seven cases of spayed dogs. Out of thirty clinical cases of mammary tumour presented, 10 cases (33.33%) have multiple growths, and 20 cases (66.67 %) have solitary growths, pedunculated growth are 12, remaining eighteen growths are sessile. Ulcerated and inflamed are 36.67 % (11/30), and non infected are 63.33% (20/30).The surface of tumour is nodular in eight cases (4.18). Recurrence are recorded in 4 (13.33%) cases whereas, first time occurrence are 26 (86.67%).

Cancer has gained considerable relevance in animals now-a-days owing to the increased awareness among people towards animal sufferings and pain. The diagnosis and management of neoplasm, therefore, represent the major challenges faced by a veterinary oncologist. Mammary gland tumours are among the most common canine neoplasm, but the vast majority of these tumours occur in intact female dogs. In canines, mammary gland tumours that occur in males ranges from 0-2.7% (average < 1%). Sex hormones plays a vital role for the occurrence of mammary gland tumours. The p53 gene over expression is an independent factor for indicator of worse prognosis in canine mammary gland tumours. The p53 may be useful prognostic markers for evaluating malignancy in canine mammary gland tumours. There is a strong positive correlation between expression of cyclooxygenase-2 (Cox-2) and grade of malignancy in the tumours. Increased Cox-2 expressions are linearly related in the canine mammary gland tumours and are also related to worse prognosis and shorter overall survival. Canine mammary gland malignant tumours have the highest values of Cox-2 expression. Matrix metalloproteinase (MMPs) are a family of zinc and calcium-dependent proteolytic enzymes that degrade macromolecules of the extracellular matrix. Members of this family such as MMP-2, -9 and -7 have been shown to be associated with tumour progression and invasion in human cancer tissues. Matrilysin (MMP-7) influence the early stage of tumourogenesis.

Cancer has gained considerable relevance in companion animals nowadays owing to the increased awareness among the people towards animal sufferings and pain. A proper diagnosis augments a better treatment regime for the suffering animal. Of all species, dog develops cancer twice as frequently as humans. Mammary tumours rank second (behind skin tumors) as the most common neoplasm in dogs but constituting more than 50% tumours in bitches. All suspected growths therefore requires careful appraisal lest it be cancerous. Diagnosis of cancer represents one of the major challenges faced by the small animal veterinarian. In majority of instances, the differentiation of a benign from the malignant tumour can be made morphologically with considerable certainty and for the same, histopathological examination remains the gold standard. A proper histopathological diagnosis however depends a lot on the collection, fixation and processing of tissues. So clinician as well as histopathologist has their own role to play in the final diagnosis of cancer in animals.

Microsatellites are highly polymorphic repetitive DNA sequences that are randomly distributed throughout eukaryotic genomes displaying high levels of variation and consequently, are ideal for deciphering genetic variability. They are having high mutation rates (1-4 per generation). Among the microsatellite, (CA) n repeats occurring about one in every 30 kb of mammalian genome constitute useful genetic markers, as their polymorphism is sufficiently stable and their mutation rate are low enough. Out of innumerable microsatellite, ZuBeCa3 microsatellite shows huge variability in the canine genome (Switonski and others 1998). This microsatellite is located in chromosome 9q21-q22 and vastly studied in different dog breeds depicting its heterogeneous distribution over the chromosome (Switonski and others 1998).

Mammary tumours arise from the ducts are called as ductal or ductular carcinoma. Some include other tissues such as the myoepithelium and connective tissue between the glands. These have a number of different names including carcinosarcoma, carcinoma or sarcoma in benign tumour, complex adenocarcinoma and malignant mixed tumour. Tumours are also classified by the type of epithelial growth because this influences the probability of recurrence. Tumours that only have myoepithelium or connective tissue are called sarcomas.

Mammary tumours are the most frequent neoplasia in female dogs; therefore, these tumours represent a serious problem in veterinary medicine. Many tumours presented activated genes which are not normally expressed in the tissue from which they originate. The proteins derived from the expression of these genes are called tumour markers. Detection and measurement of these proteins are used in medicine for diagnosis and assessing the evolution of the tumours.

MMPs are a family of zinc-containing, calcium-dependent endopeptidases secreted by various cell types and implicated in a wide range of physiological events (e.g. trophoblast invasion, development, endometrial remodeling, ovulation, angiogenesis, wound healing, bone resorption and cell migration) as well as in pathological processes. Matrix metalloproteinase (MMPs) and their tissue inhibitors (timps) have been strongly implicated in the pathogenesis of many types of human cancer. Expression levels for the most relevant MMPS are confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR). The activities of MMPs are significantly higher in malignant tissues than benign canine tumours.

Sex hormone plays a vital role in the occurrence of canine mammary cancer. It is well established that canine mammary cancer is sex-hormone dependent. The risk of developing canine mammary tumour is 0.05% if the dog is spayed before its first estrous and the risk of developing a mammary cancer is increased as the number of estrous cycle increases. Mammary cancer is often hormone responsive, since growth or regression of tumours can be modulated by suitable hormonal manipulations. Estrogen and Progesterone appear to be major hormones in regulation of mammary tumour development. It is also reported that about 40-50% malignant mammary tumour in the dog have either or both estrogen and progesterone receptors. Benign tumours in dogs have also female sex hormone receptors and they can be stimulated by hormonal cycling. Mammary carcinoma can be induced in male and female rodents by administration of estrogen. Transsexual males given high doses of estrogen to induce mammary development have developed mammary cancer. So measurement of different sex hormones or sex steroids is an essential step during any study related to development and efficacy of treatment of canine mammary cancer.

Depressed immune responses have been observed frequently in cancer patients. Cancer and other stress are known to affect different components of the immune system. Cancer treatment is relying mainly on surgery, anti-cancer chemotherapy and radiation therapy. Most chemotherapeutic agents are responsible to myeloid toxicity, which leads to leukemia and immunosuppression affecting both humoral and cell-mediated immunity. This is a very serious disadvantage of chemotherapy besides they are cytotoxic drugs.

Radiographic examination of mammary tumour patients should be done prior to any treatment to detect the chances of lung metastasis. Radiographs of the thorax helped to provide information pertaining to the extent of organs involved and the presence of metastasis in the lungs. Lateral radiograph of thorax revealed absence of pulmonary metastasis in normal cases. Lung metastasis may observe in animal characterized by radioopaque soft tissue scattered in the lungs indicating pulmonary metastasis.

Cancer, according to current opinion, is a multitude of different diseases and is no more a “one disease’. Myriads of tumour types and plethora of their patho-physiology in different species necessitates species wise study of tumours in detail. Of all species, dog develops neoplasms twice as frequently as humans, with incidence of skin and mammary tumours being the highest. Tumors of mammary gland are the most common tumors of female dog representing approximately 30-50% of all tumors in bitch and it is the second most common tumour in dog after skin neoplasms and most common malignant tumour in dog.

Tumours of mammary gland are the most common tumours of female dog representing approximately 30-50% of all tumours in bitch. Chemotherapy is a kind of treatment that uses drugs to attack cancer cells. The importance of chemotherapy has been emphasized that survival could be prolonged after chemotherapy in cancer patients.

Considering immunosuppressive nature of mammary tumours, the use of immunotherapy to prevent cyto-toxicity of cancer drugs, further metastasis and prolong survival period in canines. “ImmuPlus” (Indian Herbs) is a type of polyherbal ayurvedic product containing extracts of Ocimum sanctum, Withania somnifera, Emblica officinale and Tinospora cordifolia.

Tumours of mammary gland are the second most common tumours of female dog representing approximately 30-50% of all tumours. Chemotherapy is a kind of treatment that uses drugs to attack cancer cells. The importance of chemotherapy has been emphasized and reported that survival could be prolonged after chemotherapy in cancer patients. Clinical trials of the combination of selective COX-2 inhibitors with chemotherapy in patients with a number of cancers have been initiated and preliminary results are encouraging. The introduction of immunohistochemistry and newer techniques like nucleolar organizer region (NOR) staining have improved the objective of diagnosis of cancer. Silver stained nucleolar organizer region (AgNOR) count is considered to be valuable prognostic marker for canine malignant lymphomas and other canine tumour. This study is designed to investigate the therapeutic efficacy of chemotherapeutic agent-Methotrexate with or without COX-2 inhibitors-Meloxicam and also to study the potential clinical application of nucleolar organizer regions (NORs) as prognostic indication in canine mammary tumours.