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Crust is characterized by the presence of desquamated squamous epithelial cells. The dried exudate consists of necrotic cell debris, leucocytes, RBC’s, plasma proteins and microorganisms. It is classified into serous crust (eosinophilic protein exudates), hemorrhagic crust (RBC’s), cellular crust (degenerative leucocytes mainly neutrophils), serocellular crust (mixture of serum or plasma or leucocytes) and palisadic crust (presence of leucocytic infiltration, pustules and parakeratosis organized in a horizontal manner).

Scales are having imperfectly keratinized superficial layers of the epidermis characterized by cracks or thin flakes.

Ulcerative dermatitis is one of the primary features of the skin disease in which discontinuity of the skin along with marked inflammation and necrosis are seen.

Etiology

Canine dermatophytosis (canine ring worm) is a relatively common dermatological condition caused by Microsporum canis, Trichophyton mentagrophytes and Microsporum gypseum. These fungi are keratinophilic in nature. It can occur mostly in the face and forelegs.

Etiology

It is a rare canine dermatological condition. This condition is subdivided into two groups. In the first group, it is seen in Siberian husky and Alaskan malamutes. It is due to inherited impairment in the zinc absorption or metabolism. In the second group, it is due to use of high plant phytates diet (zinc absorption prevention), commercial or home prepared diet which is zinc deficient or diet is over supplemented with calcium and other vitamins and minerals which impair zinc absorption. Group two is mostly seen in large breed dogs and young adults.

Etiology

It is a rare idiopathic primary dermatitis condition seen mainly in Daschund.

Etiology

It is a relatively rare skin disease or dermatologic condition. Hypersensitivity reaction is one of the suggestive cause for this condition characterized by nodules or plaques which are seen in the skin, oral mucosa and external ear canal.

Etiology

It is an uncommon endocrine related skin disorder. It is very rarely encountered in neutered dogs.It is always associated with cryptorchidism and sertoli cell tumor. This condition is closely accompanied with endocrine related testicular tumor followed by skin lesion and feminization.

A very common condition caused by Malassezia pachydermatis in dogs. It is also caused by predisposing allergic skin disease. It occurs in ventral neck, ventral abdomen, axilla, face, pinnae, feet and forelegs. Tape impression smear will reveal numerous yeasts.

This tumour may occur due to specific mutation in the p53 tumor suppressor gene. This is one of the most common epithelial tumor of dog. It is classified into well differentiated, moderately differentiated and poorly differentiated tumour.

This is a benign/malignant tumor originating from the apocrine and eccrine sweat glands in dogs. It is usually recorded in the head and neck region but it may occur on any site.

Cytology reveals clusters of moderate to severe exfoliated neoplastic cells which contain round nuclei slightly eccentric in position in the light pale granular cytoplasm. In carcinomas, clusters of basophilic epithelial cells had large nuclei and prominent nucleoli. It may also contain one or two large secretory droplets.

It is one of the common epithelial skin tumors. It is more common in head but also recorded in other sites such as back, tail, limb, trunk and eyelids. It is a tumor of sebaceous cells. In sebaceous gland adenoma, it has predominantly sebocytes with less of basaloid reserve cells while in sebaceous epithelioma variant, more of basaloid reserve cells are seen with less of sebocytes.

The tumor arising out of ceruminous gland might develop subsequent to chronic otitis externa in dogs. It may be a benign or malignant tumor of ceruminous gland epithelium. It is more common in the external ear canal of dogs.

Benign/malignant tumor may arise from fibrous tissue. Fibroma mostly occurs in the dermis or subcutis of limbs and head.

The tumour is most commonly seen in adult and aged dogs. It occurs anywhere on the body surface. It is due to trauma, chronic inflammation, vaccination, genetic predisposition etc.

Lipoma is a benign tumor of adipocytes and it is more common in dogs and mostly observed in the trunk, thigh and proximal limbs with rare presence of ulceration.

Liposarcoma is a rare malignant tumor of adipocytes in dogs. Grossly, the neoplastic cells are arranged in less circumscribed to large firm to hard (Figure 67) nodules on the dermis and the nodules noticed on the subcutis are grey white in color.

It is a benign tumor of endothelial cells. It is a common tumor of dogs. Solar induction is one of the cause for development of this tumor.

Haemangiosarcoma is a malignant tumour of vascular endothelial cells. It is more commonly seen in aged dogs.

Hemangiopericytoma is a tumor originating from pericytes. It is mostly seen in distal extremities due to elevation of blood pressure in that area. It is mostly seen in skin and subcutaneous tissue around the joint region.

Histiocytoma is more common in younger dogs below two years of age. It is also observed in all age groups.

Gross examination

Grossly, histiocytoma appears as light pink, smooth to firm, raised round (Figure 77) masses. Alopecia and ulceration are also observed.

It is more common in old dogs and also mostly observed in the cutaneous region of trunk and legs.

Grossly, the tumour appears as solitary to multiple masses as white, pink to red, smooth, and raised nodules. Alopecia and ulceration are also observed. It is mostly observed in the pinna and digits.

It is more common in dogs and frequently seen in the skin and oral cavity. It usually occurs in 5 to 11 years old dogs. Benign melanomas are solitary, circumscribed, alopecic firm and freely movable blue black coloured nodules in the skin. Malignant melanoma is sessile, polypoid to plaque like grey or brown black coloured granules in the skin with ulceration.

It is commonly spread by coitus and also by licking and rubbing of the affected area. It is more common in active breeding dogs.

Etiology

It is a pruritic transmissible dermatological condition caused by Cheyletiella yasguri, one of the surface living mite.

Site of incision: Remove the abdominal viscera to expose the sublumber region.

Introduction

Biofortification is the development of nutrient dense staple food crops using the best conventional breeding practices and modern biotechnology, without sacrificing agronomic performance and important consumer preferred trait (Nestel et al., 2006). It provides a comparatively cost-effective, sustainable, and long- term means of delivering more micronutrients (Saltzman et al. 2014). This approach not only will lower the number of severely malnourished people who require treatment by complementary interventions but also will help them maintain improved nutritional status. Moreover, biofortification provides a feasible means of reaching malnourished rural populations who may have limited access to commercially marketed fortified foods and supplements. Micronutrient malnutrition (MNM) is the major constrain to provide nutritional security to world population of underdeveloped and developing countries. Among the 20 major risk factors of the global burden of disease estimates, Fe deficiency ranks 9th and Zn deficiency ranks 11th, while in the high mortality countries, including India, they rank 6th and 5th, respectively (WHO 2002).

Introduction

Subculture is key skill in bacteriological laboratory allowing us to colony propagate a pure culture in single colony. Pure cultures are essential for characterization such as identification using biochemical test or antimicrobial susceptibility testing.

Protocol

1. Label your fresh blood agar plate.

2. Select a single colony to subculture (colonies grows by clonal expansion; each colony should be a pure strain.)

3. Use the colony you have just picked to make the 1st streak on your fresh plate.

Introduction

Ammonia is the major end product in the breakdown of proteins in fish. Fish digest the protein in their feed and excrete ammonia through their gills and in their faces. The amount of ammonia excreted by fish varies with the amount of feed put into the pond or culture system – increasing as feeding rates increase. Ammonia also enters the pond from bacterial decomposition of organic matter such as uneaten feed or dead algae and aquatic plants.

Introduction

Ammonia, nitrite and nitrate are considerable in the water quality management because they can be produced a toxic depending to their form and concentration. The solubilities of nitrogen contain in water at different temperatures and pressures. Nitrogen is a basic growth substance and an important element in aquatic ecosystems as well as in the pond water. It exists variously forms in water and manly classified in organic and inorganic.

Overview of Inland Fisheries Status

In Thailand, inland capture fisheries are carried out mainly in rivers, lakes, swamps and reservoirs. These fisheries are an important source of animal protein for the rural people. Since 2005, production from inland capture fisheries has increased by almost 13 percent. Gillnets, longlines, hook-and-line, scoop nets, cast nets, and lift nets are some of the fishing gears being used. Among these fishing gears, gillnets are the most popular and efficient, particularly in swamps and reservoirs. Thai silver barb, snakehead, walking catfish, local carps, and Nile tilapia are caught. Nile tilapia, hybrid catfish, silver barb, snakeskin gourami, giant river prawn, striped catfish and striped snake-head contributed 95 percent in quantity and 92 percent in value in fresh water aquaculture.

Construction details

They had a total of 36 tanks, and most of them were made of tin. Each tank had a diameter of 6 meters, was 1.2 meters deep, and could hold up to 30 tons of water. Inside these tanks, there was a plastic lining. Additionally, there were 4 to 5 tanks made from bamboo. Bamboo tanks have a shorter lifespan, lasting 1 to 2 years, while tin tanks can last for 8 to 10 years.

Culture details

The species that was cultured over there was Red tilapia. Culture practice was intensive. The nursery-rearing period was 70 days. In the first step, fish were raised from 0.5 grams to 10 grams in a separate tank for 35 days. The second step involved the growth of fish from 10 grams to 30 grams for the next 35 days in another separate tank. The fishes were bought from outer sources and they were all male fishes.

We visited the CPF (Thailand Public Company limited) in Samutsakorn on 12/ 10/23 at 1:30 pm. The purpose of the visit was to know about the feed ingredients and production process.

1) Overview

Product like shrimp feed and fish feed are prepared by the company. Company was spread in 61 Rai( 24 acre) area. 400 employees are working in the company.Working hours is 3 shift / day and working days – 6 days / week.Shrimp feed unit have the capacity of 13,000 T/M and actual production was 7000 T/M. 8 line pellet machine was used for this purpose. Fish and frog feed unit have capacity of 11,000 T/M and actual production 7000 T/M. 6 line extruder machine was used.

Frog farming in Thailand started 20 years ago. The frogs commonly found and farmed in Thailand are the local species, Rana tigerina and Rana rugulosa. The first imported bullfrog, Rana catesbeiana was introduced in 1980 and is popular in the northern part of the country. Rana tigerina is olive brown in colour which is about 15cm long when fully grown. The adult weighs about 300g. Rana rugulosa is another local species that is olive-brown in colour with a dark head. It is about 13cm long when fully grown and weighs about 270g. The imported species, Rana catesbeiana is olive-brown to green in colour. It is 17 cm long when fully grown and weighs around 459g.

“Curriculum” is seen often as a critical issue during discussions on quality of agricultural education, being considered variously as content, as product and as process. It is easy to make the assumption that a common understanding of curriculum, but in practice this is far from the case, with a host of definitions, approaches and ideological standpoints. Even though a dialogue on curriculum theory is well established, there is still a strong tendency in practice to understand curriculum as a list of content, often developed in considerable detail, that students should be taught.

Curriculum reorientation is necessary, when any one in use is no longer responsive to the needs, interests and unable to meet the demands of the farming community to which it is intended to serve. In any case a number of factors, such as those listed below, must be taken into consideration while reorientation:

There should be an analytical assessment of the existing agricultural extension curriculum in order to justify any proposed reorientation.

Learning needs of the farm university graduates and farmers should be reviewed from the point of view of both individual learner requirements and national development needs.

As stated before, a careful assessment of the existing curriculum is essential before any modification is undertaken and must take into account the country’s education policy, political, economic, demographic, cultural, social and technological environment. Problems of farmers to be solved, needs to be satisfied, and priorities to be identified and addressed and then only the recommendations made for action.

• Content approach

We can identify several different ‘traditional’ approaches to curriculum development. The most common approach until recently is the ‘content’ approach. This is where the curriculum is basically a list of knowledge – things that the agricultural graduating learners need to know. Usually this list is made by a curriculum development committee or group of experts identified at the national level.

To explain the main concepts and elements of a participatory curriculum development process

To present practical skills and knowledge leading to the effective design of teaching and learning

To build confidence of the learners in applying new methods and approaches to teaching and field extension work.

To demonstrate application of participatory approach in development of Extension curriculum

This Toolkit emphasizes an active learning approach, based on the process of experience, reflection and action. The key principle is that people do not learn simply by passive listening. They need to be involved from beginning of the training and designing of the program.

Real benefits are obtained when we use a participatory approach to curriculum development and reorientation. Primarily, the course contents we provide and the learning will become more effective. If students participate actively in the learning process, then they are more likely to learn and teaching is more likely to be effective. They will have more ownership in the teaching, because their needs will have been identified and hopefully they will also be involved in deciding how their needs can be met. This will increase their motivation, which will help them to learn more effectively.

In the present system of curriculum changes, participation of all the stakeholders are not actively involved in developing curriculum over the years.

The motive behind conducting the workshop on Capacity Need Assessment and Development is that the existing Extension Advisory Services (EAS) needs new capacities to respond effectively to the emerging challenges in agricultural development.

Participatory curriculum reorientation awareness-raising workshop for key stakeholders: In the present study focus group discussion and workshops were conducted to know the effectiveness of present curriculum of extension education with different stakeholders (Students UG, PG and Ph.D, Extension teachers, Field extension workers and farmers). Data were collected by using schedules regarding the important contents of present curriculum and most important for extension workers in the present situation to be included in curriculum

The concept of capacity development is defined in various ways. Broadly, it is defined as a process by which skills, and knowledge are built, utilized, retained and nurtured with a view to provide an entity responding to a development challenge. Two components that are vital in any plausible definition of the concept are human skills (the ability to perform a task) and institutions (effectiveness and efficiency of organizations, systems, processes and procedures).

Cytology is the study of cells from fluid or tissue samples prepared directly on glass slides and stained for microscopic evaluation. It is also described as examination of cells without regard to the tissue's architectural details.

It is less invasive than tissue biopsies and, in some cases, it can provide a rapid diagnosis that would otherwise take several days if done by histopathology.

When is the cytology needed?

The diagnostic methods of clinical examination, radiographic evaluation, and laboratory data suggested that a diagnosis that cannot be confirmed by these methods. In that situation, cytology is very much helpful to confirm the disease processes or conditions.

Fixation can be done by either wet fixation or dry fixation. If staining is to be delayed for a few days, cell preservation enhanced by fixation. Fix the slide within 30-60 seconds.

Wet Fixation

Good smear is well spread and tongue shaped. If forced during discharge of materials from the syringe, it is sprayed on to slide.

The ideal smears will yield intact and well stained cells (Figure) should have a clear demarcation between the nucleus and cytoplasm

Poor smear will show no cellularity or damaged cells.

Adrenal Tumours

• Adrenal cortical adenoma had normal secretory cells
• Most cells had naked nuclei in the abundant cytoplasm
• Basophilic cytoplasm with abundant clear lipid vacuoles
• Round uniform nuclei

Fundamentals of writing farm stories

1. Use of plain and simple language: A farm journalist would have translated technical terms into their plain language.

2. Presentation of novel ideas: People are open to new concepts that are logically explained and will increase their return on investment as a whole.

3. Keep the message brief and to the point so that farmers can recall it. The information should be focused on the benefits that can be attained without using an excessive amount of wording.

4. Use of visuals: Visuals add interest, are more memorable, and have deeper significance. A statement is more understandable and pleasing to the eye when there are more visuals present.

5. Farmers are very receptive to messages that are recent, reliable, realistic, and relevant (the “4Rs”).

6. The audience appreciates and accepts the message that is centred on their environment, locations, and selves.

Remote Sensing in Oil Exploration

Remote sensing plays a significant role in the oil exploration industry. It refers to the use of sensors and instruments to gather information about the Earth's surface and its environment from a distance. This information can be used to locate and evaluate oil and gas deposits.

There are different remote sensing techniques used in oil exploration, including

Seismic surveys: This technique involves creating sound waves underground and recording their reflection to determine the subsurface structure. It is commonly used in oil exploration to locate potential oil and gas deposits.

Magnetic surveys: This technique uses a magnetometer to measure the magnetic field of the Earth's surface. Magnetic anomalies can indicate the presence of underground geological structures that may contain oil or gas deposits.

Gravity surveys: This technique involves measuring variations in the Earth's gravitational field, which can indicate the presence of underground geological structures.