eBook Chapters

Precision farming is one of the most scientific and modern approach to sustainable agriculture. It involves management of within field variation and helps farmers to grow crops more efficiently. Precision farming is concerned with the management of variability in the dimensions of both space and time. Variability of resources is a key factor of precision farming.   Precision farming is the application of technologies and principle to manage spatial and temporal variability associated with all aspects of horticultural production for improving crop performance and environment quality. Precision farming calls for an efficient management of resources through location specific hi-tech inventions to produce cost-effective and eco-friendly consumer- acceptable quality products (Mondal and Tewari, 2007).

The development of bio-informatics started with the working of computers and accumulation of data on genes and proteins. There are 100,000 proteins in the human body or 1 million. In the last decade the number of protein sequences gathered in databases increased tremendously, but the key to understand the function of a protein is not its sequence but its structure. Experimental determination of 3 dimensional native folded structure of a protein usually can be done via crystallography techniques. But such experiments are time consuming. Currently there are hundreds of different fully automated approaches to protein 3D structure prediction.

All proteins are polymers of Amino acids. The polymers also known as polypeptides consist of a sequence of 20 different L-m-amino acids also referred to as residues. For chains under 40 residues the term peptide is frequently used instead of protein. To be able to perform their biological function, proteins fold into one that is driven by a number of non covalent interactions such as hydrogen bonding, ionic interaction, Vander Waal’s forces and hydrophobic packing. In order to understand the functions of proteins at a molecular level, it is often necessary to determine the three dimensional structure of proteins. The modern Information Technology techniques like Artificial Intelligence have opened new pathways to researchers. Once ANN has been trained it is capable of producing accurate output for the unknown inputs. Many researchers have tried to analyze the large sequence sized gene to determine the protein, predict the secondary structure of the protein and model its three dimensional (3D) view [11].

Abstract

A Mathematical Method for Artificial Neural Networks offers an original, broad and integrated approach that explains each tool in a manner that is independent of specific ANN systems. Artificial Neural Networks are abstract mathematical models of brain structure and functions. They are nothing but a mathematical model that relies on varying the strength of connections between the internal processing elements to interpret data. They can be applied to many pattern classification problems. In this paper, it has been used to predict the level of amino acid in protein sequences. Amino acids are the building blocks of proteins. It is any molecule that contains both amine and carboxylic acid functional groups. Here, known protein sequences of ingredients are taken as input patterns. The network is trained using back propagation algorithm with sigmoid function as activation function by generating random weights. It is tested again with unknown protein sequence. Error is calculated by the difference between actual network output and desired output. If the training is successful the patterns are correctly classified. The network is trained until the error is minimum. The amino acid level is thus predicted in a particular protein sequence.

Q.1.    What is an experiment ?

    Answer
    
    An experiment is a means of getting an answer to the question that an experimentor (researcher) has in mind. This may be to decide which of the several pain relieving drugs that are available in the market, is the most effective or whether they are equqlly effective.
    
    An experiment may be planned to compare the yield of different varieties of wheat. so that the varity giving the maximum yield may be recommended to the farmers to increase their production.

1. Introduction

Milk is considered to be an adequate source of valuable macro nutrients, fat, protein, lactose. Vitamins and micronutrients making it a wholesome food. It can serve as an excellent carrier product for extra nutrient and if enriched or fortified it can satisfy the nutritional needs of the population. Soybean is an excellent source of low cost protein and has been an important nutritional component and medical investigation have revealed the great potential of soy foods for lowering blood cholesterol levels and the incidence of heart disease and cancer. The dry soybean contains roughly 40 % protein, 20% oil and 35% soluble (sucrose , raffinose, stachyose, etc. ). Aqueous extract of soya is an ideal substitute of animal milk for people suffering from allergic, gastrointestinal and other diseases peculiar to elderly people liu et al. (2004). Soymilk obtained by aqueous extraction from whole soybeans is a well known food product that is growing in popularity in many areas of the world (Hauman,1984). Soy milk is low in fat, carbohydrate, calcium, phosphorus, and riboflavin, but high in iron, thiamin, and niacin in comparison with cows’ milk. Soy milk contains higher amount of protein than buffalo milk and is deficient in sulfur containing amino acids. Soya milk is used in various products like paneer, yoghurts , cheese, tea and coffee whiteners , shrikhand, rasogolla and various indigenous milk sweets, confectionary, etc (Kale et al., 2012, Chang et al., 2005) also reported that intake of fermented soymilk improves the ecosystem intestinal tract by increasing the amount of probiotics. During the last years, soy beverages consumption has gradually increased due to their significant concentration of health promoting compounds, such as iso flavones.

Abstract

The per capita availability of fresh water in the World is continuously decreasing. It is reported that in the World about 69 percent of total water resources developed are used for irrigation, 23 percent for industries and the balance is used by the domestic sector. About 60 percent to 70 percent of water used in domestic sector is returned as waste water. Substantial quantities of water used by industries are also returned. To meet the increased food requirements of the global population, there is constant pressure for increase in food production. Since, agricultural productivity depends on availability of water; there is increased demand of water for irrigated agriculture. On the other hand, due to increased demand of water for other sectors, quantum available for irrigation sector is getting reduced. So, for meeting the increased irrigation demand, it is necessary to economise the use of water for irrigation by adopting improved technologies and improving the water use efficiencies. 

Checks and Balances in Biotechnology Applications
    
Biosafety Issues in Animal Biotechnology Application
    
World Organization for Animal Health (OIE)
    
Recombinant DNA Safety Guidelines (1990)
    
Foreign Trade (Development and Regulation) Act, 1992 (2006)(Draft Amendment)
    
Revised Guidelines for Research in Transgenic Plants and Guidelines for Toxicity and Allergenicity Evaluation of Transgenic Seeds, Plants and Plant Parts (1998)
    
Guidelines for Generating Preclinical and Clinical Data for rDNA Vaccines, Diagnostics and other Biologicals (1999)
    
The Food Safety and Standards Act (2006)
    
Transgenic Animals Bioreactors
    
First Patent in Biotechnology
    
Biotechnology - Ethical Concerns
    
Some Priority Areas in the Field of Biotechnology
    
Suggestions for Future Development of Biotechnology Activities in the Field of Animal Health and Production in India
    
Epilogue

1.    Factors Causing Spoilage of Foods
    
2.    Methods of Preservation
    
3.    Preservation by use of Preservatives
    
4.    Preservation by Irradiation
    
5.    Preservation by Low Temperature
    
6.    Methods of Food Freezing
    
7.    Principles of Preservation by Fermentation
    
8.    Preservation by Dehydration
    
9.    Preservation by Thermal Processing
    
10.    Advantages of Processing
    
11.    Demand for Processed Products
        
References

1    Vaccination schedule for cattle and buffaloes

2    Vaccination schedule for sheep and goats

3    Vaccination schedule for dogs

4    Deworming schedule for dogs and cats

5    Vaccination schedule for poultry

6    Vaccination schedule for broilers in different seasons/situations5

1.    Basic Principles
    
2.    Scale and Proportion
    
3.    Balance
    
4.    Rhythm
    
5.    Simplicity
    
6.    Unity
    
7.    Transition
    
8.    Focal Point or Emphasis
    
9.    Elements
    
10.     Variety
    
11.     Grouping
    
12.     Mass
    
13.    Sequence
        
    Further Reading

1.     Centrifuge
    
2.     Spectrophotometer
    
3.     Microscope

Conservation Agriculture (CA) is an agro-ecological approach for sustainable crop production and intensification. CA comprises the resource conserving agricultural production practices that aim to achieve more produce at less cost while enhancing the quality of the natural resource base. CA is all about providing multiple options for farmers, endowed differentially. The basic components of CA include drastic reduction in tillage, adequate retention of crop residues on the soil surface, use of economically feasible diversified crop rotations, avoidance of freewheeling and practice of controlled traffic, if possible. These elements of CA are not site-specific but represent unvarying objectives that are practised to extend CA technologies efficiently across all production conditions. No-till agriculture is considered as a revolutionary step in the direction of preventing land degradation and rehabilitation of the resilient but fragile lands. No-till agriculture together with other associated management practices such as direct seeding into loose crop residues to provide soil cover and to conserve soil moisture, judicious choice of crop rotations and agro-forestry tree species constitutes CA. CA is thus being widely accepted as an important component of the overall strategy for enhancing productivity, improving environmental quality and preserving natural resources for food security and poverty alleviation in such areas.  Recent CA trials conducted in Karnataka, Andhra Pradesh and Tamil Nadu seem to bring out that the rainfed tropical India stands to benefit more from CA than the irrigated areas.

Abstract

Arsenic, although a non-metal, is very often referred to as a heavy metal. Its provenance and contents in recently formed or least weathered soils depend on the type of rocks and intensity of geochemical processes. The variation is exemplified in shale, where As content varies from 1 to 900 mg kg-1. In highly weathered soils, where pedochemical processes exceed geochemical processes, the content is generally below the levels that are toxic to life forms. In the Bengal basin, As content is low in soils, but high in the ground water (beneath the vadose zone). This suggests that As has been sufficiently leached from soils. The solubility of most of the As-bearing soil minerals is high, and it occurs as anions (AsO33-, or AsO43-) in soil solution. The soils of the Bengal basin are alkaline in reaction, mostly fine textured with almost even distribution of particle size fractions, and moderately drained. Minerals like micas and feldspars dominate their coarse fractions, whereas illite (40-47%) and smectite (24-30%) are dominant in clay fractions. Both inorganic and organic colloids are negatively charged that cannot retain anions. In many soils in the world, leaching of As is well documented. 

Abstract: Dry land ecosystems cover about 41% of the earth’s land surface including hyper arid region. There is a considerable difference between them, in which arid region covers 7% of global lands. These are barely productive and provide a meager existence to small populations, usually nomadic with little settlements as in Thar desert. In addition to hyper arid, other types are arid, semi and dry-subhumid, which are in danger of becoming desertified. Environmental conditions are harsh affecting agriculture production. People of Indian arid zone are well acquainted about agroforestry- an age old practice in India, but maintaining only a few trees on farmlands does not serve the growing demand of fodder, fueldwood and food. Adoption of alternate land use systems like alley cropping, lay farming, tree farming, dryland horticulture etc. may intensify crop production and sustain the rural livelihoods. The selection of tree/shrub species may be based on the tolerance towards drought, salitnity/alkalinity prevailing in the region. Based on arrangements and combinations of trees/shrubs with associated crops, agroforestry are categorized into: agri-silviculture, agri-horticulture, agripasture, silvipasture, hortipasture and silviherbal. Common trees are P. cinreraria, T. undulata, Salvadora oleoides, Acacia nilotica, A. tortilis, Ailanthus excelsa, Zizyphus nummularia, Z, mauritiana etc.

Introduction

In India importance of beekeeping is for honey production and little importance has till now been given to the primary biological and economic role played by bees in the pollination of agricultural crops. Honeybees are most fascinating creatures on the earth. They provide not only honey and also other valuable beehive products such as bees wax, pollen, royal jelly, propolis and bee venom. The bee forage in good quality is the primary requirement for production of the beehive products whereas efficient management and manipulation of bee colonies can competent for more production. A wise beekeeper adjusts to colony manipulation techniques according to floral and climatic conditions. In temperate regions there is enough flora during particular period i.e. spring and summer of the year and bees collect and store food but they face protracted dearth period in winter. Under subtropical climate of the country, nectar and pollen sources are available for most part of the year which are helpful for production of beehive products with quality.

16.1 Introduction

The broad bean is a hardy plant and can withstand sufficiently cold temperatures. It is cultivated on a small scale in India but widely cultivated in Latin America. This is the only bean which is sown in autumn and is grown as a winter crop (in temperate countries). Broad bean is used as green, shelled and dry beans and as a feed for livestock. Plants have distinguishing features like square and erect growing stems which grow up to 30 cm (dwarf cultivars) to 100 cm (tall cultivars). 

6.1 Introduction

It is an important vegetable in European countries. It got its name from the capital city of Belgium since it has been grown around Brussels for hundreds of years. From Belgium it has been introduced to England (1810). Some parts of Long Island in New York are also well known for Brussels Sprout production. In India, it is widely grown in Kodai kanal and Nilgiris hills of Tamil Nadu and Maharashtra. Exact statistics regarding area, production and productivity are not available. It is a cool, moisture loving and frost resistant biennial, with axillary buds produced in the leaf axils during the first year of growth and a seed head is produced in the second year of growth. Edible part is swollen axillary bud (sprouts or buttons or mini cabbage). 

12.1 Introduction

Carrot is valued as a nutritive food mainly because of high carotene content. The edible part of carrot is modified root (conical form, i.e., enlarged fleshy taproot). Although carrot is a biennial crop but it is grown as an annual for its root. Black carrots (Asiatic carrot root) are used for the preparation of a soft beverage called Kanji, which is supposed to be a good appetizer. Red type is good for preparing various types of sweets especially Gajar Halwa in northern India. Carrot seed oil is used for flavouring liquors and commercially used in France. The taste of carrot is due to the presence of glutamic acid. In carrot roots sucrose is the most abundant than glucose and fructose. Maximum concentration of carotene is found 85-100 days after planting. Carrot seeds contain germination inhibitor named as carrotal. 

3.1 Introduction

Cauliflower is a cruciferous vegetable that is naturally high in fibre and B-vitamins. It is said that the cauliflower has originated around the Mediterranean Sea. Ancestor of cauliflower is Brassica cretica which has been descended from the wild cliff cabbage, Brassica oleracea var. sylvestris, a native of coastal region of Europe and Africa. Cauliflower is cultivated worldwide in different climatic conditions ranging from temperate to tropics during most of the cropping seasons and is available round the year. Curd is the economic part of cauliflower and is an intermediate stage between vegetative and reproductive stage. It was introduced in India during the Mughal period. In 1822 a botanist Dr Jemson, In-charge of Company Bagh, Saharanpur, U.P introduced it in India from Kew Garden London. 

7.1 Introduction

Chinese cabbage appears to be native of China. It is known to have arisen from a cross between Pak choi (Brassica rapa var. chinensis, non-heading Chinese cabbage) from south China and turnip (Brassica rapa var. rapifera) from north China when they were grown together in the city of Young-chou. Artificial crosses between these species have proved this hypothesis. Its chromosome number 2n=20 distinguishes it from cole crops. It is not a member of cole group but regarded as very closely allied crop due to similarity in breeding systems, cultivation practices, season of growing and other features besides being like a member of Brassica vegetables. In general Pe-tsai cabbage, i.e., heading type (B. rapa subsp. pekinensis or B. pekinensis) and pak-choi cabbage, i.e., leafy type (B. rapa subsp. 

9.1 Introduction

Garlic (Allium sativum L.) is one of the important bulb crops grown and used as a spice or condiment throughout India. It is derived from two words gar meaning spear and lic meaning herb. It is also important foreign exchange earner for the country. Garlic has higher nutritive value than other bulb crops. It is rich in proteins, phosphorous, potassium, calcium, magnesium and carbohydrates. Healthy garlic bulbs contain allicin, a colourless, odourless and water-soluble amino acid. On crushing the garlic bulbs, the enzyme allinase breaks down into allin to produce allicin of which the principal ingredient is odoriferous diallyl disulfide. Garlic contains about 0.1% volatile oil. 

21.1 Introduction

Globe artichoke, commonly known as artichoke, is a perennial crop grown for its flower buds. It has thistle like plants. The above ground portion dies each year during winter and again emerges in spring. It is generally propagated by means of suckers or off shoots from the old root stocks and also by dividing the old crown into pieces with a portion of the stem. The buds are harvested before they become loose and fibrous. It is very nutritious as it contains appreciable quantities of proteins, carbohydrates and minerals. It is a herbaceous perennial in which the globular immature flower heads or buds and the edible bracts are used as vegetable. 

1. Introduction

Jackfruit (Artocarpus heterophyllus Lam. ) a member of the family Moraceae is the largest tree-borne fruit in the world. India is the second biggest producer of the fruit in the world and is considered as the motherland of jackfruit. The jackfruit is native to parts of South and Southeast Asia and is believed to have originated in the rainforests of Western Ghats of India and is cultivated throughout the low lands in South and Southeast Asia. Major jackfruit producing countries are Bangladesh, India, Myanmar, Nepal, Thailand, Vietnam, China, the Philippines, Indonesia, Malaysia and Sri Lanka. There are 100-500 seeds in a single fruit (Sidhu, 2012). It is composed of rind, edible bulbs of yellow-flesh and seeds. The ripe fruits are eaten or processed into snacks and canned products. Seeds make up around 10 to 15% of the total fruit weight (30-365/ fruit) and have high carbohydrate and protein contents (Ocloo et al., 2010). Mostly the seeds, rind and strands are discarded as waste. So, the seed, rind and strand flour can be an alternative intermediatory product, which can be stored and utilized, both for value addition and to blend with other grain flours without affecting the functional and sensory profile of the final product.

Common Name: Ganth gobhi or kadam

4.1 Introduction

Knol khol or kohlrabi is not very widely grown in our country except in Kashmir, West Bengal and some of the South Indian states. Kohlrabi is a herbaceous biennial grown for its edible stem. The edible part in kohlrabi is the tuber formed by the thickening of stem tissue above the cotyledons, i.e., swollen stem called knob or tuber. 

10.1 Introduction

Leek is a non-bulb forming tetraploid plant of onion family. It belongs to monocotyledons group like onion and garlic, and has white narrow bulbs with broad leaves and etiolated pseudostem formed by the leaf sheaths. Except large size it looks like green onion. Its cultivation is not commercial in India and grown only in kitchen garden. Recently, its cultivation has been noticed in temperate regions of north India and the Niligiri hills of South India. Leek is cultivated for blanched stems and leaves. It is a hardy biennial plant, consumed as salad and used in flavouring the soups. It has flattened leaf blades rather than the radial, and it is bigger than onion. As compared to onion, it has milder and more delicate flavour. 

18.1 Introduction

It is a rich source of vitamin A as compared to spinach and carrot. It also contains high quantity of ascorbic acid, manganese and iron. A 100 g of leaves supplies as much essential amino acids as 100 g of any non-vegetarian food like meat and fish. Its succulent leaves and stems make nutritious dish after cooking. The herbaceous annual plants are mildly laxative besides other medicinal values. This is annual for leaf production and biennial for seed production. 

Abstract

Roselle, or Jamaica sorrel (Hibiscus sabdariffa L.) is a popular vegetable in many tropical regions, cultivated for its leaves, seeds, stems and calyces of which, the dried calyces are used to prepare tea, syrup, jams, jellies and beverages. It is an annual herbaceous shrub belongs to family Malvaceae originated in old world tropics. The fresh calyces and young leaves are used as a vegetable in various cuisines and the plant also yield industrial bio fibre. It also has nutraceutical potential, high pigment content, and nutritional and medicinal properties. Roselle is a drought tolerant crop and cultivated throughout the tropics and subtropics during hot and rainy seasons. China and Thailand are the largest producers and control much of the world supply, where as the best quality roselle comes from Sudan. Many medicinal applications of this plant have been developed around the world. It is used to treat hypertension, pyrexia, dyspepsia, and leukaemia hepato protective, due to its high content of protocatechuic acid. Leaves are emollient and sedative and also show antihypersensitive, antihyperlipidimic, diuretic and anti oxidant properties. Hence, it is an ideal candidate crop for low input multi-cropping system for the food and nutritional security for developing countries.

19.1 Introduction

Traditionally spinach or vilayati palak is classified as a pot herb. The word ‘spinach’ has been derived from a Spanish word Hispania. However, the Latin meaning of ‘spina’ is spiny fruit and oleracea means herbaceous garden herb. The edible part of spinach is a compact rosette of leaves which is normally consumed as a cooked vegetable. Sometimes, it is also used as salad along with lettuce and other vegetables. Spinach is highly suitable for hydroponics. 

5.1 Introduction

Sprouting broccoli is believed to be the first to be originated from wild cabbage (Brassica oleracea var. sylvestris) or wild cliff cabbage which is found growing wild in southern Europe and along the eastern Mediterranean Sea. It is also known by several other names such as Calabrese or Italian Green because it was cultivated first in Italy by the ancient Romans and spread to other countries only from sixteenth century onwards. In India, it was introduced during the second half of the 20th century. The word broccoli is derived from the Latin word Brachium meaning an arm or branch. Broccoli contains higher amount of protein, vitamin A and C and appreciable quantities of thiamine, riboflavin, niacin, calcium and iron. 

14.1 Introduction

Turnip is quick growing root vegetable. It is a herbaceous biennial for seed production and an annual for use as a vegetable. Both roots and leaves are edible in turnip. Turnip produces napiform types of roots. Turnip is a popular root vegetable of North India grown during winter months. In young stage, it can be cooked but when it is mature, it may become fibrous and hard. It can be eaten raw but, for making palatable pickles, only desi types are the best. European cultivars of turnip are sweeter than Indian ones. The turnip greens are rich in vitamin A and C and contain appreciable amount of vitamin B, and also good source of Ca, P and Fe. 

1    Consent form for surgery

2    Consent form for euthanesia

3    Slaughter certificate

4    Wound certificate

5    Proforma for export certificate of dogs

Q.1.    What is progeny row trial ?

    Answer
    
    The replicated experiment designed to compare a number of parent plants with respect to any characteristic under study is known as progeny row trial.

• A Brief Description of Visual Basic
• Types of Application in VB
• Creating the Project
• Steps in Building a Visual Basic Application
• Visual Basic Data Types
• Managing Variables 
• Constants
• String Functions
• Operators in Visual Basic 
• Controlling Program Flow
• Looping
• The InputBox( ) Function
• Methods
• Procedur
• Control Array
• Msgboxes
• Data Access
• Data Access Objects (DAO)
• Microsoft Jet Database Engine 
• Remote Data Objects
• Microsoft ActiveX Data Objects
• Data Control
• Object Linking and Embedding (OLE)

Principal Scientist and I/c Head, Division of Social Sciences and Training, IIHR, Hessaraghatta Lake Post, Bangalore – 560 089, Karnataka

Introduction

In the face of growing threat of accelerating climate change, global focus is shifting towards plant-based alternatives as a means for climate resilient ecosystems to meet the food requirements of global population. Such a change in priority is also seen in the growing global awareness towards a shift in daily ‘diets’ away from the meat-based ones towards healthier and balanced ones involving fruits and vegetables that provides cheaper yet nutritionally secure alternatives. A reiteration of this fact that speaks volumes about the changing priority is the UN declaration of the year 2021-22 as ‘the International Year of Fruits & Vegetables’ to raise awareness about balanced nutrition and to direct policy towards efficient management of production with minimizing post- harvest losses and wastage.

Introduction

Agriculture plays a vital role in India’s economy. Over 58% of the rural households depend on agriculture as their principal means of livelihood. As per the 2^nd advanced estimates by the Central Statistics Office (CSO), the share of agriculture and allied sectors (including agriculture, livestock, forestry and fishery) is estimated to be 17.3% of the Gross Value Added (GVA) during 2016- 17 at 2011-12 prices. The GDP of agriculture and allied sectors was recorded at $244.7 billion in FY 2016.

INTRODUCTION

For survival of young seedlings and rooting of cuttings it is essential that they maintain a healthy water and air balance especially to make up the water lost in transpiration. Water stress within the cuttings hinders metabolic activities and cell regeneration and consequently initiation of roots. So medium should be such that it provides adequate water required for rooting and growth. Also these substrates must provide initial nutrients necessary for plant growth. These should also contain micro-organisms that seedlings may need.

INTRODUCTION

The propagation of plants has been one of the fundamental occupations of mankind since beginning of civilization. Plant propagation involves the application of specific biological principles and concepts in the multiplication of plants for useful purposes. The present day plant propagation industry is large and complex and involves not only the group that multiplies the plants for sale and distribution, but also a large group of industries that provide services, sell the product, is involved in regulation, provides consultation carries on research or is involved in teaching. The key person with in this complex, however, is the plant propagator who possesses the knowledge and skill either to perform or to supervise the essential propagation tasks for specific plants.

INTRODUCTION

Plant propagation involves three main aspects: knowledge of mechanical manipulations and technical skills which constitutes the art of propagation; knowledge of plant growth and structures which constitutes science of propagation and knowledge of different kinds of plants and their methods of propagation. The basic objective of plant propagation is to increase the number of desirable types and to preserve the essential characteristics of the plant. Generally, sexual propagation or the seed propagation is used to raise the seedling rootstocks on which to graft or bud the desirable types. The clonal rootstocks are multiplied through vegetative means- layering, cutting etc. Various sexual and asexual methods such as use of seeds, apomicts, specialised plant parts, cuttings, layers, grafts and buds are employed to multiply plants of different fruit crops. In this chapter, the techniques used for raising rootstocks and multiplying the desirable types of different fruits have been discussed.

INTRODUCTION

With the change in world scenario from plentiful resources to limited with population increasing geometrically, shrinking land and climate change, the pressure on agriculture is mounting continuously. Meeting the nutritional requirements of population by optimum supply of food is the pre-requisite for sustainability of life under such conditions. Rapid urbanization is causing scarcity of cultivable land. Therefore, the productivity per unit area of the crops needs to be increased manifold. Horticulture, in general, has attained a pride status in the field of modern agriculture. However, radical changes are expected in horticultural practices in near future as the situation in terms of area as well as production is not as comfortable as the food grain production.

The dream of having genomes completely sequenced is now a reality. The complete sequence of many genomes including the human one is known. However, the understanding of probably half a million human proteins encoded by less than 30’000 genes is still a long way away and the hard work to unravel the complexity of biological systems is yet to come. A new fundamental concept called proteome (PROTEin complement to a genOME) has recently emerged that should drastically help to unravel biochemical and physiological mechanisms of complex multivariate diseases at the functional molecular level. Proteomics involves the systematic study of proteins in order to provide a comprehensive view of the structure, function and regulation of biological systems. Coupled with advances in bioinformatics, this approach to comprehensively describing biological systems will undoubtedly have a major impact on our understanding of the phenotypes of both normal and diseased cells. Initially, proteomics focused on the generation of protein maps using two-dimensional polyacrylamide gel electrophoresis.

If the 1990s were the decade of genomics, the first ten years of the new century are set to become the decade of proteomics. For the first time, the technologies of proteomics make it possible to generate quantitative protein expression data on a scale and sensitivity comparable to that achieved at the genetic level. This advance has major implications for our understanding of cellular organisation in health and disease and for pharmaceutical and agricultural biotechnology. Indeed, proteomics is already yielding important findings, across a wide range of applications. Most proteomics research, however, is directed towards the more proximal goal of investigating protein expression and function under specified physiological conditions.

The rapid evolution of proteomics has continued during the past years with a series of innovations in the core technologies of two-dimensional electrophoresis and mass spectrometry, and a diversity of productive research programmes. Well annotated proteomics databases are now emerging in a number of fields to provide a platform for systematic research, with particularly promising progress in clinical applications such as cardiology and oncology. Large-scale quantitative research, comparable in power and sensitivity to that achieved for gene expression, is thus becoming a reality at the protein level.

11.1 Total DNA Extraction by cTAB Method
    
11.2 Extraction, Purification Estimation of DNA
    
11.3 Potassium Acetate Procedure
    
11.4 DNA  Estimation
    
11.5 Restriction Enzymes

1. Introduction

Psyohrometrio is the study thermal and physical properties of gas-vapour mixtures. Ambient air is the mixture of dry air and water vapour and some pollutants. The moisture and pollutant content vary from place to place. The amount of pollutant and water vapour decrease with increase in the altitude. The air is completely dry above 10 km altitude. In many unit operations moist air is required and properties of air changes with temperature and pressure. The pollutants has to be removed before using moist air as a process air either by filtration or circulation. So it is necessary to determine the amount of water present in the air and effect of different unit operation on thermal properties and change in the heat and moisture content of mixture.The International Joint Committee of psychrometric data have fixed the composition of dry by mole fraction as: Oxygen: 0.2095, Nitrogen: 0.7803, Argon: 0.0093, Carbon dioxide: 0.0003. The following are the properties of the dry air

Molecular weight    28.966

Abstract

In view of the fast depletion of ground water reserves, uncertainty of rains and growing food demands due to human population explosion, efficient use of water available for crops has become highly relevant. In recent years, the use of superabsorbent polymers (saps) has been viewed as an innovative tool to improve water use efficiency in agricultural operations. Pusa Hydrogel, an indigenous superabsorbent hydrogel technology, has been developed for improving water use efficiency of agricultural and horticultural crops. They can absorb a minimum of 400 times of their dry weight of pure water and gradually release it according to the needs of the crop plant. So, it is used as soil moisture conservator and reduces number of irrigation. Because of their neutral ph, they do not affect nutrient availability, soil chemical composition, action of other agro chemicals, viz. fertilizers, herbicides, fungicides, insecticides, etc. Pusa hydrogels are found to improve the physical properties of soils. Increase in porosity results in improvement in seed germination and rate of seedling emergence, root growth and density, and reduced soil erosion due to reduction in soil compaction. It also increases biological/microbial activities in the soil, which increase oxygen/air and nutrient availability in root zone of the plant. So, ultimately it is helpful for increasing crop productivity. 

1. Introduction

Sea Buckthorn (Hippophae sp. ), a high altitudinal, thorny, temperate, deciduous, dioecious, actinorhizal shrub or small tree belonging to the Kingdom-Plantae, Clades-Angiosperms, Eudicots and Rosids, Order- Rosales, Family-Elaeagnaceae and native to Eurasean Countries like India, China, Russia, Nepal, Pakistan, etc. (Barik et al., 2016). SBT is having tolerance towards a wide group of pests and insects, can grow in extreme nutrient deficient conditions and temperature range of -40 °C to 40 °C. It contains more than 190 reported invaluable phytochemicals and has the potential for multidimensional use as food, fodder, medicine, fuel, and conservation of soil and bio diversity (Qinxiao and Hongyan, 2003; Barik et al., 2016). SBT berries, leaves, stem and root barks also contain nutrients and bioactive compounds which mainly include lipids, sugars, amino acids, organic acids, fatty acids, carotenoids (β-carotenes, lycopene, zeaxanthin), tocopherols (α β, γ, δ), sterols (ergosterol, campesterol), Vitamins (A, C, E and K), phenolic and flavonoid compounds (gallic acid, caeffeic acid, ferulic acid, isorhamnetin, kaemferol, quercetin, myricetin) and isoprenols (Tiitinen et al., 2005; Anderson et al., 2009; Barik et al., 2016). All these studies are primarily based on the prominent SBT species Hippophae rhamnoides. However, Sea Buckthorn (Hippophae salicifolia) of North East India is an underutilized non-conventional plant and its potential is not yet fully explored. Very scanty literatures are available on the identification, documentation and preliminary qualitative phytochemical characterization of SBT from North East India (Basistha et al., 2010; Pradhan et al., 2015). But there is no any report on systematic qualitative phytochemical characterization of all the plant parts of SBT from North East India in general and SBT of Arunachal Pradesh in partcular.

1. Introduction

Jackfruit is the largest edible tree borne fruit in the world (Baliga et al., 2011). According to Haq (2006) India is considered as the birth place of jackfruit. The major jackfruit producing states in India are Assam, Tripura, Bihar, Uttar Pradesh, the foothills of the Himalayas and South Indian States of Kerala, Tamil Nadu and Karnataka. Jackfruit is a fruit prized in some areas of the world and allowed to go waste in others. According to Sidhu (2012) the extent to which jackfruit is being wasted in India is not exactly documented however, the wastage is approximately 75%. On an average a fully developed fruit may weigh 10-25 kg. Seeds occupy around 10 to 15% of the total weight of the jackfruit (Bobbio et al., 1978). It is reported that jackfruit seeds contain high amount of protein, fiber and carbohydrate (Ocloo et al., 2010). The seeds are viable for not more than three or four days (Sidhu, 2012). According to Abedin et al. (2012) the industrial utilization of jackfruit pulp are varied but the seeds are discarded from those food industries. Moreover, mature fresh seeds cannot remain fresh for long as they tend to germinate easily. So, converting jackfruit seed into flour is an option in order to increase the shelf- life of the seed and can substitute various cereal based flours for developing food products. Dumpling is a type of food which is made out of small pieces of dough wrapped around a filling that may include vegetables, meat, or other ingredients mixed together.

Natural disasters are of meteorological or hydrometeorological origin. Disasters such as earthquakes and tsunamis are occasional, but rainfall-related hazards, flood or drought, are very frequent and thus call for proper monitoring of large-scale rainfall variation, and better manage- ment and planning to combat the problem(s). Variation in rainfall is very large spatially and temporally compared to any other atmospheric parameter, such as pressure or temperature. The large temporal variability in the Indian rainfall has produced frequent flood/drought and periods of above/below normal rainfall for several centuries past. Areas of high rainfall and runoff are flooded in the summer monsoon season whereas others face water stress and drought conditions. The influence of global warming on rainfall variations over India is reported in numerous observational (Singh et al., 1991, 1992) and modelling studies (Lal, 1994). 

Introduction

Rainfall is the principal source of water in arid areas, which augments soil moisture, groundwater and surface flows. Agriculture and several of the other economic activities in arid areas depend on rain. This region is devoid of any well defined perennial river system. Under such circumstances every drop of water becomes very precious. Of the total water use about 85% of water is used for irrigation and remaining 15% is used for drinking, industrial and other purposes. About 65% of irrigation water and 30-40% of drinking water is subjected to serious losses. Hence, increasing water use efficiency coupled with increasing availability of water through rainwater harvesting and management is of prime importance for sustainable development of arid areas. Rainwater harvesting, its conservation and efficient utilization can solve problem of water scarcity to the greater extend. Rainwater harvesting in small ponds (nadis), underground tanks (tankas), Khadins (Low lying areas) etc. is an age-old tradition in arid zone of Rajasthan. These traditional rainwater harvesting structures vary in design, shape and size. These traditional methods have undergone several changes for being more economical and efficient. Central Arid Zone Research Institute (CAZRI), Jodhpur since its inception in year 1959 is continuously working for development and refinement of water efficient techniques for crop production, individuals and for the communities for development in hot arid zone of India.

In our previous chapters, we have discussed various sampling designs or techniques such as simple random sampling, stratified sampling, systematic sampling and cluster sampling recently we have discussed unequal probability sampling i.e., probability proportional to size sampling. In all these sampling designs the variable of interest (generally there is only one variable of interest) which we have considered and perform our estimation based on that single variable. There are some other variables which are related to our variable of interest and if we utilize those variables, we may gain some efficiency in our estimator i.e., if we take some correlated variables with our variable of interest or study variable by this, we can enhance our estimation. The variable which is correlated with the study variable is usually known auxiliary variable or supporting variable or supplementary variable or benchmark variable. This auxiliary variable is utilized in two stages either in the design stage or estimation stage.

Graunt (1662) was the first who used auxiliary information to estimate the population size of England. After this Laplace used auxiliary information for the estimation of the population in France.