eChapters

Research is the basics which have changed human life and surrounding environment what existed on earth million years ago into present form. As soon as man realized himself as a separate entity from other animals, he started thinking about himself, creatures co-inhabiting with him and the universe. In that sense, biological science is the oldest subject which fascinated man, though it was called natural sciences in ancient times. Perhaps, the first curiosity was about himself, his birth, death and alike matters. As soon as he realized mortality of life or faced death of his dear ones, he attempted to become immortal or ‘amar’. This is reflected by worshiping of ‘Asur ’(Hirnakashyap, Bhasmasur, Ravan etc) to ‘Brahma’ or ‘Shiva’ for receiving the blessings of becoming immortal . However, even God refrained to bless these personalities becoming ‘amar’ confirming the realization by all that life is strongly linked with death ; as such they were blessed with certain conditional ties which may lead their death. Eventually, they met with their death under specific circumstances (3). When God or His little part (Ansh) took birth on earth as incarnation, He also followed all the rules and regulations of cosmic laws. But He never tried to become immortal and left His body on the earth, after finishing His duties.

The very basics in one’s life is whether he has judged his interests or decided goals of his life or has taken life casually. The first is to know what you wish to achieve in your life or in the words of our former president and Bharat Ratna APJ Abdul Kalam (14) what are your dreams ? If you have dreams than only you can achieve them hence it’s essential to know what is your passion ?, passion is the key to success. In pursuing your passion, all things become secondary ; in fact, desired changes occur within you, unnoticed and automatic to achieve your goals. It may not be out of contest to mention that basically I belonged to a business family and being eldest son in the family, my father asked me to join family business, after doing my intermediate, with the liberty to join part time BA classes while carrying business activities. But business was not my passion hence I struggled to join to the academics and carried all my Veterinary education with distinctions - because of my interests. I am not sure what would have happened to my life have I joined least interested business line- perhaps a failed business man.

At times, we hear our young generation questioning the need for obtaining degrees for conducting any research work. To some extend they are right. If we look back, there are many examples where persons without any former degree attained what is impossible by many degree holders. Tulsidas has written ‘Ramacharitmanas’, incorporating so many principles of Vedas and stories from Puranas without obtaining any degree. Srinivasa Ramanujan (21) narrated mathematics theorems without any degree in mathematics. His achievements may be appreciated by the fact that we celebrate his birthday 22nd December as National Mathematical day.

Whenever a person acquires desired qualifications, he needs a forum to express his talents and a place where he can fulfill his dreams and make important attainments. This may be achieved by two ways. One is, he becomes an entrepreneur and plans his own organization. As we are discussing biological research, there are good opportunities to start a Pharmaceutical firm to produce a drug, or a biotechnology firm to produce some biochemical molecule, enzymes, bio-fertilizers, vaccines against important human/animal/poultry diseases or a firm to supply diagnostic kits or immunological products etc. These are not hypothetical suggestions, difficult to achieve by a middle class person, but part of reality achieved by middle class family in India with laurels in their field of work. We may cite examples of firms like Dr Reddy Laboratory (Dr K.A Reddy), Biocon India (Ms Majumdar), Venky (poultry vaccines etc), Biorad (Bangalore based biochemical molecules supplier) who are working in India and leading their respective field. All this have been possible with tremendous change that has occurred in Indian scenario where there are many venture capitalists (or PE firms) who are willing to invest any amount of money provided they like your new idea of establishing a new enterprise. A somewhat more detailed information, in this regards, is provided elsewhere (see Research for money-chapter 13). Here I have not mentioned to pursue a Dairy or Poultry farm as these are not parts of research activities but related to animal production and any other person may also enter in such commercial activities.

It will be unjust not to describe history of Parasitology (31) and a little bit about Parasitology (chapter 8) so that our students may know the earlier works done in this field and how research has advanced by diligent observations of the eminent scientists when they were not having such sophisticated facilities.

As helminth is visible with naked eyes, it was this parasite which man encountered first in its journey of life sciences or natural sciences,as was known in ancient times. Some of these were known even before the era of biblical Palestine and ancient Egyptian civilization. Hippocrates (460-357BC), ‘Father of Medicine’ knew about pinworms (Oxyuris equi) of horses. In his “History of Animalium”, Aristotle (384-322 BC)(32) has mentioned three kinds of helminthes-flatworms, cylindrical worms (Ascaris lumbricoides) and thinworms (Enterobius vermicularis). The treatises of India had the information on large roundworms of intestine (Ascaris), pinworms, leeches, bed bugs and mosquitoes.

Our nature is the greatest or only laboratory where a large number of events are occurring at every fraction of a second - there are so many events, creatures, miseries taking place in the nature. Rightly it is considered an unparallel and biggest laboratory, man has ever witnessed. However, understanding it’s any event requires deep devotion and understanding,then only success will come. Our Philosopher and Poet Kabirdas has understood these facts as have reflected in his various couplets “Jin khoja tin paiya, gahre pani paith” or “chalti chaki dekh kar diya kabira roye, do patan ke beech main sabit bacha na koi“. For an ordinary person earth is still flat and he may not know the reasons for change of seasons.

Parasitology is the branch of biological sciences that deals with the study of parasites and all the related aspects. This leads to a basic question who is a parasite ? In fact, this term is frequently used in our society for those persons who are doing nothing and depending on others for their survival. The term Parasite means the organism (parasite) which depends on other organism (host) for its nutritional requirements. By definition, it excludes the organisms which are free living and do not depend on other organism for their food requirements. There are other details in fulfilling its nutritional requirements and important one is that in doing so, it causes some harm to the host other wise it will not be a parasite but a commensal.

A close look on three disciplines of Parasitology reveal that these are the branches where a scholar initially select them as per his basic qualifications. But while pursuing research in Parasitology, the line of demarcation becomes blurred and it is in fact combination of a group of scientists,from varied disciplines, who are able to solve any one problem; at that point, it is absurd to divide the subject as medical or veterinary or basic parasitology. In fact, these will be your interests and experiences which will decide your line of research and will determine your achievements.

It is always advantageous if there is a senior scientist,a mentor or a Guru ( I have not used the term teacher as all may be teachers but may not be Guru as per Indian mythology) who can recognize your talents and may believe that you may achieve some unusual goals, if properly guided or encouraged. This is important for carrying an important task to its fruitful results. Here what we are referring a Guru is not in the sense as is presently used for searching a god father. There is a vast difference between the two. The perception about god-father is that he may not possess the required knowledge but is perfect in manipulating situations in his favor so that work is done by all means. In scientific world, this is mostly applied to the persons who have contact with higher ups helping in getting promotions or foreign trips. These god fathers are good only to provide some material gifts but are not best in their scientific achievements hence cannot replace a Guru or a good scientist.

If you have prepared yourself to make research as your career, it is most advantageous to read research papers/reviews/chapters/books published by the eminent scientists. It is not necessary to confine reading to your subject but a voracious reading will enable you to develop an idea about general problems related to science and our society; how scientists managed to solve them and with development of new knowledge, how you may tackle fresh or existing problems. A deep analysis may be done of those works which are so accurate that even after lapse of so many years, they still hold true. In our opinion, it is better to launch websites/blogs highlighting important works of eminent Parasitologists for the benefit of our research scholars; a beginning, in India in Parasitology, has been made in this direction with launch of a website on first time Rafi Ahmed Kidwai award winner (in Animal Sciences) late Dr SC Dutt (www.drscdutt.com). 

One prime fact in research is not to take the matter for granted and do not believe in status quo. This is most critical mindset for creating something new. Even change may be made in existing circumstances with minimum requirements. Here, I may cite my experience while starting research in 1976 for my doctorate program on immunity against schistosomiasis. The experimental model was albino mouse where schistosomes, by their nature, develop into blood vascular system of the host hence require a special technique called “Perfusion technique” for retrieval of mature and immature schistosomes. This ‘perfusion technique’ requires use of a “Peristaltic pump” and was followed in some western countries (2). However, No body in India followed this perfusion technique hence recovered schistosomes from mouse or from any other host species by teasing the blood vessels or/and cutting and soaking liver,spleen, kidney, lung pieces in normal saline.

There is only one type of scientific research and that is to solve a real problem or to understand the universe and provide a solution –either with the experiments or by putting a hypothesis. However, we have classified the research in various types as per our comfort. But for the employed researchers, it may be a matter of concern if they are working on time bound research schemes as their services may be co-terminus with the project and they may face the problem of un-employment, after termination of the scheme. According to the scholars’ activities and type of research being carried out in the country, we can discuss the subject under following topics.

When you are assigned with your thesis problem or some problem is puzzling you, the first step is to search the related literature ; here the term literature is used not as it is used in humanities but this means the literature related to the research work published by the earlier scientists on the topic. The searching of literature is a most important and first step in conducting research on a particular topic. This not only acquaint you with the work of other scientists who tried to solve the problem but also confirms the possibility that previous scientists might have solved that problem and yours will be a repeat attempt ; in that case you may tackle other aspects of the problem, if at all they are existing and important in present circumstances.

Review of literature or consulting of literature is just beginning of a research project. The main intellectual work is to study the previous work meticulously and to draw conclusions regarding existing problems. This leads us to another intelligent part of the research i.e. planning of the research work. If you analyze what you are planning, it is only about materials to be employed and methodology of the research work (with planning of the work)- only these three parameters are under your control as results will be the outcome of these three facets while discussion will be how you consider your results in presence of previous research work. In fact, this statement of mine is more align to the sermon of “Shrimad Bhagwat Geeta” (5) where it is stated that man has control only on his actions (planning and conducting research) hence fulfill this duty without caring for the results-which in turn will depend on your analyzing the previous facts and how you have planned to solve the problem. Therefore, pay full attention on these three facts of the research.

After planning research problem, the next step is to start real research work. Indeed, it is said that planning of a research work is only 20% of work done while conducting the experiments is 80% to be done and is more laborious, requiring extended time and patience of the scientist. While conducting your research work,it is essential to record each and every observation of your work. These observations will be of two types. One is the subjective observations which are difficult to quantify. For example, if you are recording clinical parameters of a disease,you may record pulse, temperature, respiration in arithmetic form but clinical symptoms are subjective and require a description of the patient rather than filling tabular sheet. In describing morphology of a parasite both parameters are needed ; you will describe morphology of the parasite and will also mention measurements of different parts of the parasite. Likewise, you may like to describe weather conditions or behavior of animals, vectors at certain stage of disease while you have to mention figures in many cases. Thus your observations are clearly divided in two categories – one is subjective observation and other is recording arithmetic values.

What so ever you are doing as a researcher, one important task is to publish the same for scientific fraternity for establishing your view points else no body will recognize you and your competence. Your credentials are judged by the research papers published by you and its impact on scientific community. Therefore, it is must to publish what so ever you are doing to establish yourself in the scientific community and also to advance science. This implies to select a national or international journal for your research publication which has wide scientific circulation so that peers of scientific world may observe your publication. Adversely, the publication may bring you criticism if it does not stand on scientific parameters and you may lose your reputation in your profession.

What so ever work you have done, it will not be recognized in the scientific world unless you publish it hence publishing the search work is of prime importance for a scientist. As it provides you recognition, you must pay greatest attention on quality of your research work which depends on the keenness of your observations. It is must to confirm and re-confirm your observations prior publishing them as once they are published, it will come in public domain with nothing you can do (except of publishing another paper to refute your earlier finding). As publishing the observations will form opinion about your scientific integrity and knowledge among the peers, the paper, if not cared and contained silly technical mistakes, will reflect ignorance of the workers on the subject.

It is a must for the research scholars to attend scientific conferences of the Parasitology associations, after being members. It is advisable to become life-members of all three Parasitology associations, in India i.e. Indian Society of Parasitology, Lucknow, Indian Association for Advancement of Veterinary Parasitology, IVRI, Izatnagar and Indian Academy of Tropical Parasitology, JIPMER, Pondicherry. The annual conferences expose better chances to interlink with Parasitologists of the country and at times foreign scientists.

As the Veterinary colleges, and Parasitology being an integral part of them, are part of Agricultural universities framed on USA land grant pattern, or separated as Veterinary university imbibing the same philosophy, extension is an integral part of the system. Here, a team of the experts visit the villages to teach the villagers about the basic problems, faced by them in day to day dealing with animals. For explaining a particular problem to the villagers, the experts generally take help of the pamphlets which they have prepared. These pamphlets or extension literature deal the subject with common sense and illustrate the material with line drawings.

Till twentieth century information technology was restricted to European and American countries but entering in twenty first century witnessed passage of this technology with a vast speed in all other countries making a spectrum of changes in communication and transmission of knowledge that has altogether changed scenario of whole world. Earlier communication to international scientists was costly, time consuming while now its only a click away. So is of transmitting reprints of research papers – earlier they were dispatched by mail, now these reprints and other matters are available in soft copy and are attached with e-mail that is transmitted instantly.

Indian Agriculture is facing a wide spectrum of constraints such as burgeoning population, shrinking farm land, restricted water availability, imbalanced fertilization, low soil organic carbon, besides experiencing the fatigue of green revolution and vagaries of climate change. To address all the challenges ahead, we should think of an alternate technology such as “nanotechnology” to precisely detect and deliver the correct quantity of nutrients or other inputs required by crops that promote productivity while ensuring environmental safety. The word “nano” refers to the size of one-billionth of a metre or one-millionth of a millimeter in any one of the dimension. We are aware that all materials are made up of atoms which are the smallest units and for example, ten hydrogen atoms put together will measure one nanometer. As the size gets reduced, the surface area gets increased by several folds.

Introduction

Nature has been performing wonderful feats for millions of years. All organisms from microbes to humans are powered by highly evolved molecular and cellular machines that operate at nano scale. The capability to manipulate, control, assemble, functionalise, produce and manufacture things at atomic precision provides an opportunity to understand this new and exciting field of science. Nanotechnology is application of science to control the properties and structure of matter at nanometric scale.

Introduction to Nano-materials

Nano-sized materials are naturally present from forest fires and volcanoes, but are also generated unintentionally from anthropogenic sources as a by-product of combustion and deliberately as manufactured nanomaterials. Nanoparticles have been used for centuries. The coloured glass that we see in many old cathedrals from the middle age was made of gold nanosized clusters that created different colour depending on the size of the nanoparticles. The most prominent example of engineered nanoparticulate material is carbon black which has been around us for decades in applications like printing inks, toners, coatings, plastics, paper, tires and building products.
 
Two of the major factors why nanoparticles have different properties (optical, electrical, magnetic, chemical and mechanical) than bulk material are because in this size-range, the quantum effects start to predominate and the surface area to volume ratio is increased.

Introduction

The metal particles in nanometer scale shows exciting optical and electronic characteristics when compared with respective bulk metals. Because of the inherent electronic properties of the metal and metal oxide nanoparticles these nanostructure materials are applied in various disciplines in recent days. Mostly these nanoparticles can be synthesized either top down or bottom up approaches. The conversion of bigger size particle or matter into tiny particles is called top down approach where as smaller particles or atoms joined together forming particles in regular shape within nanometer scale dimension is called bottom up approach. The formation of nanoparticles by chemical reduction is an example for the later one and the reduction size with the help of mechanical crusing leads to the formation of fine nanoparticles is named as top down approach. Mostly nanoparticles can be synthesized by chemical method because of its easiness to synthesis particles with uniform size and high purity.

The history of nanotechnology in the modern world dates back to 1959 when American physicist Richard Feynman inspired the scientific community in his lecture “There is a plenty of rooms at the bottom”. Research on nanosized particles dramatically developed into an important field of modern research with potential effects in electronics to medicine and recently in agriculture and food industry.

Nanoparticles exhibit unique properties in terms of chemical, physical, photoelectrochemical and electronic properties when compared to their respective bulk materials. The applications of these nanosized particles are defined in a bigger ways in the field of Biotechnology. All these applications require custom based synthesis methods for specific applications.

Nano-material possess unique properties such as dimension, shape, specific surface area, surface mass ratio etc., that are highly influenced by the mechanical and physical properties. It is pertinent to understand the basic physical properties of nano-materials in order to apply them in a wide array of functions. In this context, this chapter is devoted to provide basic information on physical, mechanical and photo-luminescence properties of nano-materials.

Nano-materials are to be extensively studied and characterized prior to their application in material science, biological science, electrical and electronics. In addition to the basic physical properties of the nano-materials, other characteristics such as paramagnetism, ferromagnetism, supermagnetism, optical properties, quantum size effects and electrical properties have to be studied. With a view to provide the fundamental information on these aspects this chapter is dedicated.

Raman Spectroscopy

It is a spectroscopic technique based on inelastic scattering of monochromatic light, usually from a laser source. Inelastic scattering means that the frequency of photons in monochromatic light changes upon interaction with a sample. Photons of the laser light are absorbed by the sample and then reemitted. Frequency of the reemitted photons is shifted up or down in comparison with original monochromatic frequency, which is called the Raman effect. This shift provides information about vibrational, rotational and other low frequency transitions in molecules. Raman spectroscopy can be used to study solid, liquid and gaseous samples.

Wilhelm Conrad Roentgen, German physicist, born on November 8, 1895 discovered X-rays and was awarded the first ever Nobel Prize in Physics in 1901 in recognition of the extraordinary services he has rendered by the discovery of the remarkable rays subsequently named after him. These short-wave electromagnetic radiations have the wavelengths that are matching with both the atomic sizes and shortest interatomic distances. But, the index of refraction of X-rays is near unity for all materials and hence they could not be focused by a lens in order to observe small objects such as atoms, as it is done by glass lenses in a visible light microscope or by magnetic lenses in an electron microscope. Thus, in general, X-rays cannot be used to image individual atoms directly. As an alternative to this difficulty, Max von Laue in 1912 proved that the periodicity of the crystal lattice allows atoms in a crystal to be observed with exceptionally high resolution and precision by means of X-ray diffraction for the first time by using a single crystal of hydrated copper sulfate (CuSO₄ 5H₂O).

Electron microscopy is a specialized branch of science, which uses the electron microscope as a tool. The electron microscope is an instrument which magnify smallest object thousands times. The magnification is not only requirement to see the smallest otherwise invisible object but the details of the object are most important. The details of the object are depending upon the resolving power of the microscope, which is known as the resolution of the microscope. This resolution or power of the microscope is depends upon the wavelength of the illuminating source. The smallest the wavelength of the illuminating sources is the best resolution of the microscope. Electron microscopes use electrons as an illumination source to take advantage of the very short wavelengths attainable in electron beam, which permit a considerable improvement in resolution over the light microscope.

Electron Microscopes are scientific instruments that use a beam of highly energetic electrons to examine objects on a very fine scale. This examination can yield information about the topography (surface features of an object), morphology (shape and size of the particles making up the object), composition (the elements and compounds that the object is composed of and the relative amounts of them) and crystallographic information (how the atoms are arranged in the object).

Electron Microscopes were developed due to the limitations of Light Microscopes which are limited by the physics of light to 500x or 1000x magnification and a resolution of 0.2 micrometers. In the early 1930’s this theoretical limit had been reached and there was a scientific desire to see the fine details of the interior structures of organic cells (nucleus, mitochondria...etc.). This required 10,000x plus magnification which was just not possible using Light Microscopes.

Transmission electron microscope (TEM)

The transmission electron microscope (TEM) operates on the same basic principles as the light microscope but uses electrons instead of light. What you can see with a light microscope is limited by the wavelength of light. TEMs use electrons as “light source” and their much lower wavelength makes it possible to get a resolution a thousand times better than with a light microscope.

We can see objects to the order of a few angstrom (10^-10 m). For example, we can study small details in the cell or different materials down to near atomic levels. The possibility for high magnifications has made the TEM a valuable tool in both medical, biological and materials research.

Introduction

Biological systems are complex and are full of mysteries. Understanding function in relation to structure is crucial in many biological systems. A microscope is used to magnify, resolve and visualize a substance that is impossible to see by naked eyes and plays a vital role in various biological studies. We can visualize macroscopic organisms like animals, plants, and insects by the unaided eye but not bacteria, viruses or individual proteins. Biological structures have sizes variable over such a wide range that is not possible for a single microscopic technique to analyze all of them. Therefore, different types of microscopes with varying magnification and resolving abilities were developed to unveil the structural complexities of biomolecules (Fig. 1). Over the last 100 years, several types of microscopes have been designed and used in various fields. The microscopes work on different principles, and have a broad range of applications.

Introduction

Gas chromatography–mass spectrometry (GC-MS) is equipment that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample. Applications of GC-MS include drug detection, environmental analysis include pesticides residue in soil, water and plant crop produce, greenhouse gas quantification, food toxicants, assessment of antibiotic compounds, estimation of volatile organic compounds from biological systems besides identification of unknown compounds.

Introduction

Seed is the key input in agriculture deciding the fate of productivity of any crops. Indeed, seed is referred as a nature’s “nano-gift” for agriculture. Seed is a smart delivery system where we shall make manipulation to achieve the desirable goals. The seed technologists in the country were trying to find a second or third generation technologies to address the issues of seed science. Nanotechnology is one of the cutting edge research areas that has enormous potentials to solve the unresolved field problems. The application of nanotechnology to the agricultural and food industries was first addressed by a United States Department of Agriculture roadmap published in September 2000. Nanotechnology in the agricultural front has been more useful in improving the existing crop management techniques. Most of the applications of nanotechnology spearhead around nano encapsulated agrochemicals, herbicides, nano coated gene delivery and nano fertilizers (Nair et al., 2010).

Introduction

Fertilizers play an important role in improving soil fertility and productivity of crops regardless of nature of cropping sequence or environmental conditions. It has been unequivocally demonstrated that one third of crop productivity is dictated by fertilizers besides influencing use efficiencies of other agri-inputs. In the past four decades, nutrient use efficiency (NUE) of crops had hardly exceeded 35-50%, 18-20% and 30-35 % for N, P and K, respectively, despite our relentless efforts. The remaining nutrients stay in soil or enter into the aquatic environment causing eutrophication. In addition to the low nutrient efficiencies, Indian agriculture facing a problem of low organic matter, imbalanced fertilization and low fertilizer response that eventually caused crop yield stagnation (Biswas and sharma, 2008).

Introduction

Weeds are unwanted plants in agricultural production causing huge losses in crop production system particularly rainfed agriculture. Of the total estimated losses caused in crop production by pests, diseases and weeds in the world, the weeds alone are responsible for one-third of it. Weeds interfere with crop growth and cause an yield loss to the tune of 10% of crop production which amounts to a total loss of about 20 million MT of food grains, valued at over Rs.10,000 crores, at current prices in India. Besides, losses of similar magnitude are experienced in vegetable, fruit and plantation crops. The losses inflicted on biodiversity, environment and health are considerable and have not been quantified scientifically.

Nanotechnology is an emerging of field of science which is being highly exploited in the sphere of medicine where “nano” plays a vital role in delivery of drugs or chemical molecules to the point it is required in précised quantities in right proportions. Nanoscience infuses intelligence to the truck load of chemical constituents that are to be delivered at appropriate locations and cleave from the site after the task is complete. Such process will likely to reduce the cost besides ensuring environmental safety. Nanoscale devices with its unique properties make the agricultural system more smart and effective; such devices are capable of responding to different situations by themselves, thus taking appropriate remedial action with the need of external directions from humans. In short, these devices act as a detectors and if need arises serve as a solution/remedy for the particular issue. These smart delivery systems of chemicals in controlled and targeted manner can be considered synonymous to the proposed nano-drug delivery system in human (Patolsky et al., 2006).

Nanofibres are an exciting new class of material used for several value added applications in the field of such as medical, filtration as barrier in composites, garments, wipes, insulation, and energy storage. Special properties of nanofibres make them suitable for a wide range of applications from medicine to consumer products and industrial to high-tech applications. The US - National Science Foundation (NSF) defines nanofibres as having at least one dimension of 100 nm or less (Hegde, 2005). Amongst the various nanostructures that have recently been developed for use in practical applications, nanofibres produced from synthetic and natural polymershave received increased attention due to their ease of fabrication and the ability to control their compositional, structural and functional properties (Burgeretal.,2006).

Nanotechnology offers unique opportunities for the development of novel materials and applications. It is set to offer a platform to revolutionize agriculture sector from production, protection, processing and storage (Kuzma and Verhage, 2006). Bulk of nanomaterials produced is used in other sectors; only ten per cent is of use in food, beverages and packaging sector. Studies have projected the opportunities that nanotechnologies might bring to developing countries in the fields of water, energy, food and agriculture and health (Salamanca- Buentello et al., 2005) The key contributions due to application of nanotechnology in agriculture are delivery systems that aid in slow release and efficient delivery of agroinputs.

Background

The demand for development of Integrated Pest Management (IPM) strategies is increasing, since many problems have appeared with the use of synthetic pesticides and semiochemicals – informative molecules used in insect-insect or plant-insect interaction – are being considered as an alternative or complementary approach within IPM strategies. (Heuskin et al., 2011). Indeed, such communication molecules like para-pheromones do not present any related adverse effects on beneficial organisms in the ecosystem.

For fruitflies management, several eco-friendly control options  have been developed and recommended across the globe which  include trapping, safe disposal of infested fruits, repulsion with botanical products and physical covering of the developing fruits, besides area-based control through mass trapping and sterile-insect technique. Nevertheless, the use of para-pheromones-based lures in trap systems appears to have greater potential to be among the more widely adopted practices (Verghese, 2009).

In recent years application of nanotechnology in various fields including agriculture has received increased attention. Nanotechnology offers an important role in improving the existing crop management techniques especially in plant disease management by controlled and targeted release of agrochemicals to enhance their efficiency. The nano-based delivery systems are beneficial because of improvement in efficacy due to their higher surface area, higher solubility, induction of systemic activity, higher mobility and lower toxicity (Sasson et al., 2007). The pharmacokinetic parameters of nano particles may be altered according to size, shape and surface functionalization. They can also be used to alter the kinetic profiles of drug release leading to more sustained release of drug there by reducing the frequent application (Sharon et al., 2010). The successful use of metal nanoparticles in medical streams as antimicrobial agents has also led to their applications in controlling phyto pathogens (Nair et al., 2010).

Introduction

Since past decades, the application of nanotechnology in the areas of medicine, materials science and electronics have been widely accepted and appreciated due to its potential and benefits.  However, only during the recent years, researchers from other disciplines have started to see the potential applications of nanoscience in the field of agriculture (Robinson and Morrison, 2009). Recently, a generalized and process based framework to enable identification and characterization of the outputs (publications, patents etc.), and map them to the different agricultural research theme areas through the filter of links in the agri-value chain were developed by Kalpana Shastri et al., (2010) from National Academy of Agricultural Research Management (NAARM), Hyderabad, India. The frame work also permits assessing the implications for technology transfer, and impacts on society and environment. This frame work mainly relies on the identification of nano research and relating them agri-food thematic areas (Fig.1).

Introduction

Biosensor technology is a powerful alternative to conventional analytical techniques, harnessing the specificity and sensitivity of biological systems in small, low cost devices. Despite the promising biosensors developed in research laboratories, there are not many reports of applications in agricultural monitoring. The authors review biosensor technology and discuss the different bio-receptor systems and methods of transduction. The difference between a biosensor and a truly integrated biosensor system are defined and the main reasons for the slow technology transfer of biosensors to the market place are reported. Biosensor research and development has been directed mainly towards health care, environmental applications and the food industry. The most commercially important application is the hand-held glucose meter used by diabetics. The agricultural / veterinary testing market has seen a number of diagnostic tests but no true biosensor systems have made an impact.

Introduction

Crops diseases are caused by bacteria, virus, fungi and phytoplasmas. The detection of pathogenic bacteria and viruses in plants, planting material, vectors or natural reservoirs is essential to ensure safe and sustainable agriculture. The molecular based techniques, have evolved significantly during recent past that  allows the rapid and reliable detection of pathogens. The detection and diagnosis of plant pathogens is essential to take control measures and subsequently for eradication of the disease that causes.  Molecular detection is largely based on PCR or RT-PCR amplification following purification of nucleic acids from the samples, with the extraction of the target DNA.

Introduction

Plant diseases are one of the causes of concern in agriculture and horticulture industry. Estimated global crop loss due to plant diseases exceeds hundred billion US$ worldwide (Orke et al. 1994; Narayanasamy, 2010). Plant diseases are caused by microorganism such as fungi, bacteria, viral, viroids and phytoplasma. Most of the plant pathogens are transmitted through seed or planting material, which can spread secondarily through vectors and lead to severe loss to the crop. Among plant diseases, viral diseases cause serious crop losses and affect the quality of products, while the use of virus- free seeds and planting stocks results in a substantial increase in agricultural crop production (Waterworth & Hadidi, 1998; Strange& Scott, 2005).

Introduction

Spoilage in agricultural products due to mould growth can occur at various stages of production and storage and is significant in terms of trade economics, food safety and public health (Shephard, 2008). It has been reported that 5–10 % of agricultural products in the world are spoiled by mould contamination to the extent that they cannot be consumed by humans or animals. Mould growth decreases the quality of food and also creates a potential risk for human health because of the production of toxic secondary metabolites known as “mycotoxins”. Foods contaminated by mycotoxins, when consumed by humans and animals causes a disease called “mycotoxicosis”, resulting in death (Wagacha and Muthomi, 2008).

Introduction

Fungicides are biocidal chemical compounds or biological organisms used to kill or inhibit fungi or fungal spores. An insecticide is a pesticide used against insects. Pyrethroids are widely used insecticides in agriculture as they control pests and diseases effectively in plants. Because of their low biodegradability and high persistence, they are extensively found in environmental samples such as air, water, soil, sediments, food and biological tissues [1-4]. Moreover, these chemicals induce cancer and act as endocrine disrupters in several organisms due to their high degree of toxicity. Although the use of most pyrethroids has been prohibited or limited in developed countries, they are used in many developing and under developed countries for controlling of pests and insecticides [5-8].

Introduction

Fruits are important sources of vitamins, carbohydrates, fiber and sugar. A large variety of fruits are grown in India, of which mango, banana, citrus, guava, grape, pineapple and apple are the major ones. In India, grapes occupies fifth position amongst fruit crops with a production of 16.77 million tonnes from an area of 0.64 million ha (FAO, 2009). The total grape export from India during the year 2007-08 was 96,723 tonnes worth Rs 317.84 crores. (APEDA, 2009). Grape is an excellent source of many nutrients and phytochemicals, able to contribute to a healthy diet. Grapefruit is a good source of vitamin C and pectin fiber. The major varieties of grapes grown in India are Thomson seedless, Sonaka, Anab-e-Shahi, Perlette, Banglore blue, Pusa seedless, Beauty seedless etc. (NRCG, 2009). Red grapes are grown in large quantities, especially for wine and juice production. Grapes are used for making jam, juice, jelly, vinegar, wine, grape seed extracts, raisins, grape seed oil and some kinds of candy.

Introduction

Food packaging is a critical technology addressing the ever-increasing demands for convenience, freshness, ease, shelf-life, safety and security of food products.
 
The main purpose of food packaging is to protect the food from microbial and chemical contamination, oxygen, water vapor and light. The type of packaging used therefore has an important role in determining the shelf-life of the food. Nanotechnology can be intervened to achieve the purpose of food packaging and it will become one of the most powerful forces for innovation in the food packaging industry.