eChapters

3.1 Introduction
The mammary glands of mammals produce milk, an opaque milky liquid. It is the primary source of nutrition for young mammals before they are capable of digesting other forms of food. The immunoglobulins are transported to the young ones by the early secretion of udder immediately following parturition, which is referred to as colostrum. It has the potential to mitigate the risk of numerous maladies in newborns. The precise composition of fresh milk varies depending on the species, but it contains substantial quantities of calcium, protein, and fat. The mammary glands of mammals produce the opaque white liquid known as milk. Up until they are capable of digesting other types of food, it is the primary source of nutrition for juvenile mammals. Milk serum is defined as milk plasma minus casein micelles, a composition that is similar to that of whey.

The major constituents of milk are
Water is the medium through which the other milk constituents are dissolved or suspended. The majority of the fat in milk is present in the form of small globules, with an average size of 2 to 5 microns (with a range of 0.1 to 22 microns). This is known as milk fat (lipid). Fat is present in an oil-in-water emulsion. Milk fat is composed of numerous fatty acid glyceride esters; when it undergoes hydrolysis, it produces a mixture of glycerol and fatty acids. Proteins from milk: Proteins present in milk: Protein is one of the most intricate organic substances. Proteins are composed of numerous amino acids; some amino acids are essential, while others are not. In order for the body to synthesise proteins, the diet must include the essential amino acids. The preponderance of the proteins in milk are composed of whey proteins, beta-lactoglobulin, and alpha-lactalbumin. Milk is the primary source of casein, a calcium caseinate phosphate compound.

Nutrition involves various bio – chemical and physiological activities which transform feed elements into body elements. These feed elements are nutrients which are digested, absorbed, utilized to build and renew the components of the animal body. As a result, animal grows and produce – milk, eggs, wool with the help of energy so produced in the body. After weaning, most of our farm animals obtain all of their feed supply from plants. Barring carnivores, the plant kingdom is the original and essential source of all animal life, because plants are able to utilize the energy of the sun to build substances which nourish
the animal. Plants make use of carbon dioxide, water and mineral salts to form carbohydrates, fats and proteins, which are utilized in the life processes of animal body. Thus, plants store and animals dissipate energy.

Soil is one of the major platforms on which life sustains. Soil, the product of weathering, covers the earth’s surface. Soil is the medium of plant growth and thus it requires proper attention for better crop yield. The land is the constituent of soil, vegetation, physico-chemical and biological attributes affecting its usages. The soil may act as a sink or source for the elements and other materials. Hence, it requires to understand the various components to facilitate management options for improving the fertility status of soil.

2.0 Soil

The soil is a natural dynamic body on which plant grows. It is a complex mixtures of minerals, water, air, organic matter and microorganisms. Besides, there exist an unconsolidated mineral or organic material on the surface of the earth and thus becomes a natural medium for the growth and nutrition of the plants.

Soils vary greatly in their organic matter content. For all practical purposes the organic matter content of the soil parallels the soil N content. The C/N ratio of soil organic matter generally falls in the range of 10 to 12. Because of the ease with which Kjeldahl determination can be made, N is often used as an index of soil organic matter content.

3.1. Composition of Organic Matter in Soil

Organic matter constitutes 1 to 6% of the topsoil weight of most upland soils. In a typical grassland soil, 5 to 6% of organic matter may be present in the top 15 cm. Top soils with less than 1% organic matter are mostly limited to desert regions. On the contrary, the organic matter content of soils in low, wet areas may be as high as 90% or even more. Soils with more than 12 - 18% organic carbon (approximately 20 - 30% organic matter) are called organic soils.

Proper soil management without impairing soil health is the prerequisite for achieving higher productivity from agriculture land. The arable soils of tropics and subtopics are poor in organic matter due to high temperature and intense microbial activity. The importance of organic matter in improving soil fertility through maintenance of optimum levels of soil organic matter as well as plants nutrients is well recognized. There is need to encourage more ecofriendly and productive farming system.

A growing number of farmers, ecologists and scientists are coming to conclusion that conventional farming can not be sustained because in the end it does more harm than good and it is high time to think of sounder ways of farming which are healthy for the soil, environment, plants, animals and human beings.

The practices of economically sound, viable and culturally appropriate agriculture is termed as sustainable agriculture. The excessive and imbalanced use of chemical fertilizers and pesticides on soil and plant in not only harmful to soil microflora and fauna but also reduces the productivity potential of the land. The conventional intensive agriculture has depleted the soil organic matter content and plant nutrients besides the susceptibility of crops to pest and disease. Nitrates the ultimate product of N- fertilizers pollute the ground of water and food, has higher levels of non-protein nitrogen (NPN). Increasing levels of NPN in human bodies have been reported. Soil is a dynamic living system and if it is not manured to feed soil biota, soil health gets deteriorated which is essential for cycling of plants nutrients.

Abstract

Modern agriculture largely depends on use of high cost inputs and energy intensive farm machinery. Worlds increasing population and continuously deteriorating soil fertility has alarmed agriculture system to look for some safe alternatives. Organic manures are rich source ofnutrients and humans, therefore crop residue recycling by composting is a best alternate of modern high cost input based agriculture. Compost is an aerobically decomposed organic matter .Bacteria, fungi, actinomycetes algae and protozoa are involved in the process of composting and are found throughout the natural environment. The composting material warms up due to microbial activity and it’s temperature becomes up to 160 ⁰F active stage of microbial activity . There are four types of compositing i.e. Windrow composting, Aerated static pile composting, In-Vessel composting and Vermi-composting. The C: N ratio, aeration, temperature and moisture are major factors affecting composting process. Major advantages of composting are: Aggregate formation, Reduction in bulk density, Increase in hydraulic conductivity, Reduction in loss of soil moisture, Increase in organic carbon content and pH of soil.

Composting explains the biological decomposition of organic materials and its importance for soil fertility, nutrient recycling, and sustainable farm management. The chapter introduces stages and types of composting, including anaerobic, aerobic, and vermicomposting systems, and discusses physical, chemical, biological, economic, and social benefits. Major methods such as Indore, Coimbatore, Bangalore, NADEP, sugarcane trash composting, fermented mixed compost, and crop-residue composting are presented. Factors affecting decomposition, including carbon-to-nitrogen ratio, shredding, moisture, and temperature, are examined. The chapter concludes with vermicomposting, earthworm roles, materials, preparation, developmental phases, and essential conditions required for effective vermicompost production. with emphasis on practical farm-level management practice systems.

Compounding in Hospitals
Compounding in hospitals is an essential pharmaceutical practice that involves the preparation of customized medications to meet the specific needs of individual patients. This process, which includes mixing, altering, or assembling drug ingredients, addresses scenarios where commercially available drugs may not be suitable. It requires significant expertise and compliance with regulatory standards to ensure patient safety and the efficacy of compounded medications.
Importance of Compounding in Hospitals
The role of compounding in hospitals cannot be overstated, as it enables healthcare providers to:
• Personalize Treatment: Patients may have unique requirements that standard medications cannot meet. For example, children and elderly patients often need doses that are not commercially available, and compounding allows for the creation of appropriate, smaller dosages.
• Formulate Alternative Dosage Forms: For patients who have difficulty swallowing pills, hospital compounding can provide alternative forms such as liquids, creams, or gels.
• Omit Allergens: Many commercially available drugs contain dyes, preservatives, or other additives that some patients cannot tolerate. Compounded medications can be formulated without these substances to meet the needs of sensitive patients.
• Create Specialized Medications: In cases where patients require treatments not available in mass production, such as certain cancer drugs or emergency antidotes, hospital compounding offers a practical solution.
• Address Drug Shortages: Hospitals often rely on compounding to prepare medications during periods when commercially manufactured drugs are unavailable due to supply chain disruptions.

Since the wide introduction of organochlorines and other synthetics in early 50’s farmers have heavily relied on the chemical pesticides for pest control. Over emphasis on chemical control has caused serious imbalances in the agro-ecosystem. The enormous quantities of chemicals, poisonous to microbial life, that fall on earth gets mixed up well with soil. Some problems like non-target effects of chemicals as well as latrogenic diseases are being experienced. Coupled with these, the increasing awareness of impeding pollution, health hazards and development of resistance have intensified the search for alternative strategies.

In the present investigation, the attempts have been made to test the stability and pest management efficacy of the plant extracts of some Indian medicinal plants, namely, Datura stramonium, Ricinus communis, Vitex trifolia and Nerium indicum. In the earlier chapters detailed methodologies and the results have been elaborated. This chapter embodes the comprehensive inter- and intra-comparative results and discussion on the basis of selected plants, extraction media, pest species and bioassay followed by conclusions.

The hot sterilized water extracts of R. communis were found to be more stable (i.e. 20 days to start the fermentation). In overall study the plant extracts were stable or viable means active for three to four weeks only. In most cases, the activity of cold and hot water extracts was found to be increasing with fermentation however the decrease in the activity of some extract was also observed.

The highest mean anti-fungal activity was reported for R. communis extracts followed by D. stramonium and N. indicum aqueous extracts, whereas no any anti-fungal potency was reflected by V. trifolia against test fungi species. R. communis and N. indicum extracts, the cold water method extraction showed greater positive effects in terms of mean anti-fungal potency as compared to the hot water extracts. The water extraction of D. stramonium was found to be more effective in anti-fungal activity study.

COMPOSITION OF DIET

Objectives

• To study the general considerations on feeding aspects of laboratory animals.
• To get acquainted with the specific nutritional requirements of laboratory manures.
• To familiarize with the watering system.
• To know the daily feed schedule of different species of laboratory animal.

Materials Required

Laboratory animals, feed ingredients, water, green vegetables.

For having good growth and production, proper balanced diet should be fed, thus nutrients can be classified into 6 classes:

Theory

Area computation is an important part of surveying. The term ‘Area’ is meant the projected area on a horizontal plane. The area is computed from the following methods: (i) Graphical method (ii) Instrumental method and (iii) Use of formulae. In graphical method, the area can be computed by dividing the whole area either into triangles or squares. The area of individual triangle is computed from base and the altitude and summation of individual area gives the total area. A tracing paper is divided into number of small squares is placed over the plan. The number of complete squares within the boundary is counted. The squares more than half are counted as full squares and those less than half are neglected. The total number of squares multiplied by the area of each square gives the total area of the field. In instrumental method, the planimeter is used to measure the area of a plotted plan. In use of formulae, computation of area by formulae depends on the shape of the field.

Heat units also called growing degree-days (GDD), effective heat units, or growing degree units, are a heuristic tool in phenology. GDD is a simple means of relating plant growth, development and maturity to air temperature. The concept is widely accepted as a basis for building phenology and population dynamic models. Degree day units are often used in Agrometeorology, Agronomy, Entomology and Pathology essentially to estimate or predict the lengths of the different phases of development in crop plants, insects, pathogens etc.

As an engine requires fuel to run, similarly the animal body requires food to run its vital activities. Food, after its digestion, absorption and oxidation, provides energy to the body. The energy thus obtained is utilized by the animal for maintenance, growth, reproduction and production. Hence, a good quality of food should be provided to the animal so that it may fulfil all the requirements of the animal. Food contains water, carbohydrates fats, proteins, vitamins and minerals which are essential constituents of the body and indispensable for life. Water being an essential constituent of protoplasm carries out many other important and inevitable functions in the body. Water helps the body absorb nutrients from the food and throw out wastes from body in the form of sweat and urine. Carbohydrates, fats, and proteins provide energy to the body. Fat provides 2.25 times more energy than carbohydrate and proteins. Besides providing energy, proteins are also essential for body building, growth and repair of wear and tear of the body tissues. Vitamins protect the body against diseases and help in keeping eyes, gums, skin, teeth and bones healthy. Vitamins are required by the body in small quantities. Vitamins are essential for normal growth, health and reproduction. Prolonged deficiency of vitamins causes characteristic diseases. Minerals required in small quantities are essential for normal growth of body and maintaining good health. Skeleton system of the body contains principally Calcium and Phosphorus with small amount of Magnesium.

4.1 Ration and balanced ration

A ration is the feed allowed for a given animal during a day of 24 hrs. The feed may be given at a time or in portions at intervals. Balanced ration may be defined as the ration that provides all essential nutrients to the animal in such a proportion and amounts that are required for the nourishment of the particular animal for 24 hrs.

Introduction

In recent decades, severe weather and climatic occurrences have become more frequent, affecting human populations across the globe. These changes are caused, in part, by anthropogenic climate change. Vulnerable communities are particularly affected by these rapidly changing patterns, which can have catastrophic consequences. To mitigate the impact of climate change, communities must have access to accurate and up-to-date climate information. Weather predictions, from short-term forecasts to long-term scenarios based on data from previous decades and centuries, can provide valuable information to socioeconomic sectors that are vulnerable to climate change. Climate services offer scientifically-based data and forecasts that can assist decision-makers in managing the risks of climate variability and change. Globally accepted indices generated from daily temperature and precipitation data can indicate the more severe features of climate change. These indices are developed and analyzed by the CCI/WCRP/JCOMM Expert Team on Climate Change Detection and Indices (ETCCDI) and have made a significant contribution to the Intergovernmental Panel on Climate Change (IPCC) Assessment Reports.

Introduction

Until the 20thcentury, bacterial infections were the number one cause of human death. In the 1940s, antibiotics became the lifelines for treating serious infections that saved millions of lives. Despite the advantages derived from the use of antibiotics, resistance to these drugs emerged. Unfortunately, resistance to most antibiotics emerged in time-course even for the most powerful antibiotics developed.Resistance to antibiotics is a challenge to the scientific community in keeping up the pace of antibiotic discovery.World Economic Forum’s Global Risks Reporthas listed antibiotic resistance as one of the greatest threats (WEF, 2020).United Nations (UN) has warned that drug-resistant diseases could account for 10 million deaths each year by 2050. By 2030, antimicrobial resistance (AMR) could likelylead up to 24 million people into extreme poverty(IACG,2019). Currently, at least 7,00,000 people die each year due to drug-resistant diseases (IACG,2019). The emergence and widespread of antibiotic-resistant organisms is a global challenge and needs a multi-dimensional approach to address the fundamental issues to minimize drug-resistance.The Antibiotic Resistance Genes Database (ARDB) contains a repository of around 23,000 antibiotic resistance gene or protein sequences conferring resistance against over 240 antibiotics (ARDB). Computational Biology (Bioinformatics) has become one of the important tools in studying antimicrobial resistance. It employs a range of computation techniques to make predictions about the biological phenomenon.

INTRODUCTION

The role for economic theory is no longer simply to explain how the existing system works, but also to explore how the economic system can be changed to become more adaptive and resilient in the face of the challenges of the 21st century, and how it can be more directly designed to support human well-being, in the present and the future. Simultaneous changes are needed, in both the actual economy and also in theoretical approaches of economics. The economic theory that was accepted as standard in the non-communist world during the second half of the twentieth century erects serious impediments to meeting the challenges of the twenty-first century. In order to redirect economics to be more useful, and more truly reflect the world we now face, a good starting point is to go back to the goals that are embedded in economic thinking. Here it is useful to make the distinction between intermediate and final goals. Final goals are ends that are worth achieving in themselves, while intermediate goals are pursued because they are expected to contribute to the final goals. As final goals for any economy are ‘well-being and equity in the present’ and second is ‘productive capital for the future’. Productive capital needed to maintain well-being also necessarily includes natural resources, as well as human health and education, and cohesive social systems. Increasing production and consumption are important intermediate goals for humankind in some times and places. Other obviously critical intermediate goals include climate mitigation, and promoting resilience and adaptation within both human society and ecological systems. Responding to global inequity as well as global climate change requires coordinated public policy to fundamentally redirect existing economic systems. In addition to a much greater emphasis on equity, economic theory that is appropriate to the needs of the twenty-first century will need to be more concerned with the meta-externalities of the economic system. The business sphere is currently creating meta-externalities of culture and politics that place great burdens on the core sphere, and that tie the hands of governments. This does not necessarily imply that the for-profit sphere is “bad” and the others “good”—rather that institutional changes must be made to bring the purposes and functions of all three spheres into better harmony. To address such a complex issues, economic theories and models that developed in the past should embedded with new advent in computational technologies. In venturing a vision of technological possibilities rather than simply projecting linear or exponential changes in performance, it is crucial to think not only of how technical improvements lead to the substitution of a new generation of tools for existing ones, but also of how entirely new uses, and indeed new needs, might emerge.

Abstract

In order to fill the gap between the protein sequences and their 3D structures available in the public databases, the computational prediction of protein structure has become the need of the hour. Therefore, the present chapter aims to highlight various methods and strategies for computational protein structure prediction by established ab initio, threading and homology modeling methods. Ab initio, threading and homology modeling methods are mainly based on the low sequence similarity, similarity between 25-30% and similarity more than 30% with the template structure respectively. Hybrid methods are preferred which club together a number of features such as structural alignments, solvent accessibility and secondary structure informations.

Abstract

Advances in genome sequencing technologies have made it possible to uncover the whole genome sequences of numerous organisms from microbes to higher eukaryotes. Haemophilus influenza, a free living bacterium, was the first complete genome to be sequenced in 1995 by a team headed by J. Craig Venter and Hamilton Smith at The Institute for Genomic Research (TIGR). Immediately after this, Mycoplasma genitalium, a bacterium responsible for reproductive-tract infections and well-known for having the shortest genome of all free-living organisms was sequenced. Subsequently, TIGR unearthed the genome sequences of many microbes like Methanococcus jannaschii, Archaeoglobus fulgidus, Helicobacter pylori, Borrelia burgdorferi etc. During the years 1996-2000, the complete genome sequences of several model organisms such as bacterium Escherichia coli K-12, baker’s yeast Saccharomyces cerevisiae, nematode Caenorhabditis elegans, fruit fly Drosophila melanogaster etc were published. Since then the complete genome sequences of many other eukaryotes have been released by the genome sequencing community.

Abstract

Food dehydration is an oldest method to increase the shelf life of food by reducing the water activity. A dried food product provides advantages of increased shelf life and reduced cost due to decreased weight as well as transportation. However, there is a decrease in product quality due to inadequate control on drying process parameters such as drying temperature and moisture removal together with change in thermo-physical properties of food. Computational modeling and simulations can provide a unique insight on the drying parameters that influences the product quality, and optimize the process conditions which are difficult by experimental analysis. Moreover, the computational techniques are advantageous over experimental optimization methods by minimizing the time and cost. This chapter reviews different computational modeling techniques such as, finite element modeling, finite volume and finite difference methods for modeling of food dehydration process. Also, this chapter describes the application of modeling techniques for determining the influence of shrinkage and porosity during dehydration.