eChapters

The body condition scoring (BCS) is a subjective estimate of the energy reserves in adipose tissues of an animal. It is an acceptive, non-invasive, quick and inexpensive method to estimate degree of fatness. It is a means of accurately determining body condition of dairy cows, independent of body weight and frame size. It is evinced that the profile of lactating cows in milch barn is different e.g. some may be in early lactation and the others may either be in mid or late lactation. The observations on body condition status of animal will help the manager to adopt corrective management measures, thus enabling to improve the production profile of an animal. Hence, body condition scoring may be used as an efficient tool in this regard. Although many may consider this as a nutritional management practice, but changing BCS have implications on milk yield, health, reproduction, longevity and overall profitability of an animal. Body Condition Scoring (BCS) is a system used to evaluate the overall health and nutritional status of animals by 

INTRODUCTION

Iron in the terrain’s crust is more oxidized now than it was when life evolved. It has been hypothesized that dissolved ferrous iron could have been a convenient oxygen acceptor and that deposition of oxidized iron in sediments must have taken place before oxygen could have entered the atmosphere in significant quantities. Obviously, during early evolution on this planet, the essential elements are present only in reduced form. Today a number of elements are present only in a highly oxidized form and considerable energy must be expanded to reduce them to a form similar to that on early earth which plants can utilize.

Iron represents essential elements which are usually available in oxidized form but must be reduced, often using metabolic energy, before they can be utilized. For the first time, Gris (1844) observed yellowing of grapevine leaves in absence of iron in the nutrient solution in which these plants were growing. Later, iron was reported to be essential for diverse group of plants. Due to a great variation in iron requirement by different plant species, it is difficult to decide whether iron is a macro or micronutrient.

Repairing fractures in small animal’s presence challenges to the veterinarians in their day to day service. These challenges include properly repairing fractures, each of which is unique, choosing the right fixation device, keeping an extensive inventory of costly instruments, and providing high standards of care for fracture repair. But fracture repair also gives veterinarians the opportunities to do something a little unusual in daily practice and to experience the immediate gratification of easing pain and promoting activity in injured animals.

Successful management of fractures mainly depends upon early diagnosis, type of fracture, timing of surgery and selection of proper method of fixation. The fixation of fractures can be achieved either by external or internal fixation methods. An ideal method of fracture fixation is one that can maintain early anatomical reduction, minimal soft tissue trauma, rigid stability and early motion of bone and joint. Restoring a fractured bone’s normal shape is important for return to function because it contributes to normal alignment of adjacent joint surfaces. Whereas, rigid stability of fracture fixation eliminates fragment movement and reduces pain. Soft tissue trauma adjacent to fractured bone should be minimum, which play a vital role in early vascularisation and prevents infection. Finally, early return to function maintains muscular tone of the limb allows normal weight mobility and joint nutrition and uses normal weight bearing forces to maintain bone density. These can be achieved with use of internal fixation devices.

Collection of blood Blood sample should be collected in a capped clean and dry vial containing an appropriate amount of anticoagulant. The commonly used anticoagulants are ethylene diamine tetra acetic acid (EDTA) @ 1-2 mg/ml and heparin @ 5 I.U./ ml of blood. Sites for blood collection Before collection of blood clean the site of collection by applying some antiseptic solution. If the area is hairy shaving of the site under aseptic condition should be done. Tip of the ear should be preferred for prompt or early diagnosis of haemoprotozoan infections. The vein can then be punctured with hypodermic needle and the collected blood is transferred to a clean glass vial. For collection of blood various preferred sites in different animals are as under

INTRODUCTION

Coral reefs are fragile and most diverse and valuable ecosystems on earth. The stony corals are formed from the tiny, soft colonial organisms called polyps. They provide enormous ecological, economic services, and cultural value to hundreds of millions of people. They are significantly contributing to the nutrition, economic security and protection from natural disasters. They are often termed as the rain forests of the sea.

Coral reefs were first appeared 485 million years ago during the early Ordovician period. They belong to the class Anthozoa of animal phylum Cnidaria, which includes sea anemones and jelly fish. Coral reef is an under water ecosystem which is characterized by reef-building corals. Unlike sea anemones, colonies of coral polyps secrete a hard exoskeletons called reef which is made up of calcium carbonate to support and protect the corals. These reefs grow best in warm, shallow, clear, sunny and agitated water.

BIODIVERSITY

The term biodiversity was coined by Walter G. Rosen in the year 1985. Biodiversity refers to the variation among living organisms that can be found in a particular area such as terrestrial, marine and desert ecosystems and the ecological complexes to which they are a part. The study of biodiversity helps to understand the ecological stability, economic and ethical importance. Coral reefs are believed to have the highest biodiversity on the planet even more than a tropical rainforest. Though the coral reefs distributed in less than one percent of the ocean floor, they provide habitat for more than 25% marine life with more than 4,000 world’s fishes. They act as protective nurseries to many fish species, and shelter small fishes to grow. Coral reefs diversity is so rich that we do not have a firm count on all the species that live within it and every year discover new species.” There are about six thousand coral species in the world, in which few are growing in warm shallow waters near coastlines while others in the dark, cold seafloor of the open ocean.

1. If AAGCT is the base sequence on one side of DNA molecule, what will be complementary sequence on the other side?

a) UUGCA

b) TTCGA

c) AAGCT

d) GGTCA

2. The start codon in the mRNA sequence is

a) AUG

b) UAG

c) UGA

d) UAA

Factorial experiments provide an efficient method for studying two or more factors simultaneously at different levels. This chapter introduces the concept and notation of factorial arrangements and explains treatment combinations in experiments such as 2 × 2 and higher-order factorials. It distinguishes among simple effects, main effects, and interaction effects, emphasizing the importance of interactions in interpreting biological responses. Factorial experiments allow researchers to examine the effects of individual factors as well as their combined influence. The chapter also explains that factorial treatments may be arranged using different basic experimental designs, such as Completely Randomized, Randomized Block, or Latin Square designs, according to experimental objectives and conditions.

Azolla is a free-floating water fern that floats in water and fixes atmospheric nitrogen in association with nitrogen fixing blue green alga Anabaena azollae. Azolla fronds consist of sporophyte with a floating rhizome and small overlapping bi-lobed leaves and roots. Rice growing areas in South East Asia and other third World countries have recently been evincing increased interest in the use of the symbiotic N2 fixing water fern Azolla either as an alternate nitrogen sources or as a supplement to commercial nitrogen fertilizers. Azolla is used as biofertilizer for wetland rice and it is known to contribute 40-60kg N/ha per rice crop. The agronomic potential of Azolla is quite significant particularly for rice crop and it is widely used as biofertilizer for increasing rice yields. Rice crop response studies with Azolla biofertilizer in the People’s Republic in China and in Vietnam have provided good evidence that Azolla incorporation into the soil as a green manure crop is one of the most effective ways of providing nitrogen source for rice.

12.1    Shelf Life
    
12.2    Pre-Treatments Vs. Shelf Life/Storage (Hot Water, Fungicides, Coatings and other Chemicals)
    
12.3    Surface Coatings
    
12.4    1-MCP Gas Exposure
    
12.5    Use of Botanicals
    
12.6    Other Chemicals/Methods
    
12.7    Evaporative Cool Storage
    
12.8    Modified Atmosphere Storage (MAS)
    
12.9    Controlled Atmosphere Storage (CAS)
    
12.10    Vacuum Packaging
    
12.11    Hypobaric Storage
    
12.12    Irradiation
    
12.13    Chilling Injury (CI)

Meteorological hazards are severe and extreme weather and climate events that occur in all parts of the world, although some regions are more vulnerable to certain hazards than others. Meteorological hazards include cyclones, droughts, floods, hurricanes, hailstorms, heat waves and tornadoes. The hazard is distinguished from an extreme event and a disaster. An extreme event is simply an unusual event; it does not necessarily cause harm. A disaster is an event that does cause harm in significant amounts. Meteorological hazards are often called as natural hazards. The natural hazards become natural disasters when people’s lives and livelihoods are destroyed.

Hydrological hazards are the result of either excess or a severe lack of water, i.e., flood or drought. Hydrometeorological hazard is a process or phenomenon of atmospheric, hydrological or oceanographic nature that may cause loss of life, injury or other health impacts, property damage, loss of livelihoods and services, social and economic disruption, or environmental damage. Human and material losses caused by natural disasters are a major obstacle to sustainable development. By issuing accurate forecasts and warnings in a form that is readily understood and by educating people about how to prepare against such hazards, before they become disasters, lives and property can be protected.

Biotechnology is being harnessed in various aspects of the livestock industry to hasten breed development for improved animal health and welfare, enhanced reproduction, and improved nutritional quality and safety of animal-derived foods. Various biotechnology methods are used in improving the breeding stock of animals. These include artificial insemination (AI), embryo transfer (ET), in-vitro fertilization (IVF), sexing of semen and embryos, micromanipulation of oocytes/ embryos, transgenesis, somatic cell nuclear transfer and the emerging technology on genomics and marker-assisted selection (MAS). Artificial Insemination: One of the earliest perfected technology is artificial insemination (AI) where new breeds of animals are produced through the introduction of the male sperm from one superior male to the female reproductive tract without mating. AI reduces transmission of venereal disease, lessens the need of farms to maintain breeding males, facilitates more accurate recording of pedigrees, and minimizes the cost of introducing improved genetics. Various technologies have evolved that led to the efficient use of AI in developing desired livestock, including the methods of freezing semen or cryopreservation and sperm sexing.

Carbon is found in all living organisms and is the major building block for life on earth. It exists in many forms, predominantly as plant biomass, soil organic matter, and as the gas CO₂ in the atmosphere and dissolved in sea water. Carbon cycles globally among five distinct pools among which the largest oceanic poolis estimated at 38000 Pg (Pg= petagram =1x10¹⁵g=billion tonnes) and is increasingat the rate of 2.3 Pg C yr^-1 (Fig. 1). The geological C pool, comprising fossil fuels, is estimated at 4130 Pg, pedologic, estimated at 2500 Pg to 1m depth, atmospheric pool comprising 760 Pg of C with nearly all of it as CO₂, and the smallest among theglobal C pools is the biotic pool estimated at 560 Pg. The pedologic and biotic C pools together are called the terrestrial C pool estimated at approximately 2860 Pg.

Agricultural research has been primarily focused to assure food self-sufficiency and security, and little concern on nutritional value “nutritional security” or health promoting qualities of food being produced. Food security has three dimensions, namely: (1) endemic hunger caused by poverty-induced under-nutrition and malnutrition; (2) hidden hunger caused by the deficiency of micronutrients like iron, iodine, zinc and vitamin A in the diet; and (3) transient hunger caused by natural calamities or civilian conflicts. Micronutrient malnutrition is recognized as a massive and rapidly growing public health issue especially among poor people living on an unbalanced diet.
 
Although great progress has been made in reducing the prevalence of hunger, over 805 million people are undernourished in 2012-14, still unable to meet their daily calorie needs for living healthy lives. This number has fallen by 100 million over the last decade (Table: 1 & Table: 2; Source: FAO, 2014). Undernourishment refers to food intake that is insufficient to meet dietary energy requirements for an active and healthy life. About one in nine people go to bed daily on an empty stomach. In cases where food is available, often the quality of the food does not meet micronutrient (vitamin and mineral) needs. More than two billion people continue to suffer from nutritional deficiencies such as vitamin A, iron, zinc and iodine. Despite progress, the number is still high, and marked differences across regions persist. 

Edible oil scenario

Vegetable oil or edible oil is the main source of energy in humans and it also supplies nutrients for growth and maintenance of human body. Edible oil demand in India has been increasing progressively since last one decade, primarily due to population growth, improved standard of living and also changes in food habits. Presently, the area under oil seed production is between 26 and 27 million ha with an average yield of 1000 to 1100 kg ha^-1. This accounts to 7.5 to 8.0 million tonnes of vegetable oil production locally, as against the current demand of 22.5 to 23 million tonnes per annum.

The Indian Council of Agricultural Research (ICAR) has developed various commercial farming-based livelihood models to enhance the income of farmers, promote sustainable agricultural practices, and improve the overall
socio-economic conditions in rural areas. While specific models may vary by region and crop, here are some key features and themes observed in ICAR’s initiatives:
1. Integrated Farming System (IFS) Models
The Indian Council of Agricultural Research (ICAR) has developed a wide range of Integrated Farming System (IFS) models tailored to various agroclimatic zones of India. These models aim to enhance farm productivity,
ensure sustainable livelihood, and improve resource use efficiency by integrating different components like crops, livestock, poultry, fishery, horticulture, agroforestry, etc.
Key Objectives of ICAR’s IFS Models
• Enhance income and employment from small and marginal holdings.
• Ensure year-round productivity and food security.
• Recycle resources within the farm to minimize external input dependency.
• Promote sustainability through organic integration and ecological balance.

The growing chemicalization of milk presents a great challenge to the dairy industry. A number of chemical compounds get entry into milk, intentionally or un-intentionally, through their direct or indirect usage for crop, animal; or dairy husbandry practices, as environmental contamination. Several of these contaminants may be naturally present on grazing grass or use as pesticides for green fodder or they may get entry through use of compounded animal feeds. These eventually get excreted into milk as residues and pose problems to food safety. The risk of health hazards arises from veterinary drugs, residual pesticides, heavy metals, detergents and micotoxins etc. It is therefore, become necessary to detect these contaminants in order to make food safe for human consumption.

Detection of Aflatoxin M 1 in Milk

Material requirements : Glass column(30 cm x 1 cm), Silica gel (63-200 µg particle size), volumetric flasks, beakers, micro pipette, HPLC equipment fitted with C 18 column(25 cm x 4.6 mm) and fluorescence detector.

Reagents: Sodium chloride solution (400g in 1000ml water), acetonitrile/water mixture (25:75), sodium sulphsate, chloroform, toluene/acetic acid mixture (9:1), acetonitrile/diethyle ether/hexane mixture (1:3:6), chloroform/acetone mixture (8:2), n-hexane and aflatoxin standard ampule.

Preparation of stock solution : Transfer contents from 2.5ml or 0.5ml ampoule, quantitatively, to 25ml or 50ml volumetric flask respectively. Rinse ampoule with small quantities of chloroform and collect rinsing into flask. Makeup the volume with chloroform, mix well and store at 5º C or below. These solutions will contain 1 µg per ml aflatoxin M1.

Working solution : Transfer 0.5ml of stock solution to a 10ml conical flask. Evaporate contents to dryness using inert gas. Dissolve residue in 10ml of acetonitrile/water mixture. Prepare series of dilutions containing 0.0025, 0.005, 0.01 and 0.025 µg/ml aflatoxin. Similarly treat 5ml of stock solution to give a concentration of 0.5 µg/ml.

Introduction

In the post-world trade organization (WTO) era, the fantastic imagination of “one world for self-reliance”, has been shattered by the Corona induced calamity and the expansionists approach of a few rouge nations. Though the global welfare and peace has always been part of Indian traditional philosophy, approach and the concept of self-reliance, the practical boundary of self-reliance in its present context, starts from the country level itself. The idea of self-reliance at multiple levels (individual, family, society, and nation) has driven human civilization for most of its efforts, inventions and actions across the world.

A very high dependency of a large number of population on the natural resources in the developing world calls for proper management of natural resources for self-reliance. Since the early days of civilization, natural resources in the form of land, soil, water, biodiversity and climate have remained the very basis of supporting and sustaining life of human beings, plants and animals on the earth. However, in recent times intensive use and over-exploitation of pristine resources have robbed them of their legendry resilience. To effectively tackle the complex problems and to fulfil basic needs of livelihood, food security, poverty, employment, equity and environmental services, efficient and judicious utilization of natural resources for enhanced and sustained productivity is a matter of serious concern for policy makers, planners, scientists, conservationists and environmentalists.

Introduction

The term ‘multipurpose trees’ covers all woody perennials used in agroforestry. Many authors have given many definitions of MPTs and MPTS. Burley and von Carlowitz (1984) reviewed many definitions and gave a synthesised definition which states that “multipurpose trees and shrubs (MPTS) are those which are deliberately grown and kept and managed for preferably more than one intended use, usually economically and/or ecologically motivated major products and / or services in any multipurpose land use systems, especially agroforestry systems”. The Forestry /Fuelwood Research Development Project (1992) precisely defined ‘multipurpose trees’ (MPTs) to mean tree species that are grown to provide more than one significant crop or function on the farm.

INTRODUCTION

One of the most dangerous ecological crises being faced by all living organism now-a-days is the pollution of the environment. It is easier to describe pollution rather than to define it. It is presumed that the environment in past was more or less pure. But due to various activities of man such as industrialization, urbanization, agricultural practices etc, composition of the environment gets changed which is called environmental pollution. Any substance that creates pollution is called ‘pollutant’. There are different types of pollution among which pollution caused by toxic level of heavy metal pollutants is called heavy metal pollution.

Heavy metal pollution is a problematic field of research and is gaining immense importance now-a-days. We have 104 elements in the periodic classification of which 80 are metals. According to Passow et al. (1961), heavy metals are those having densities more than five (5 g cc^-1) and atomic weight twenty three. The term ‘heavy metal’ is imprecise but is widely used although others such as ‘toxic metal’, ‘trace element’ are possible alternatives. Sources of these heavy metals are varying; some of these are encountered in water, air effluent, solid waste and sewage. They are emitted out from various sources e.g. waste water from electroplating industry (Cr, Ni), air emission from fluorescent lamps (Cd, Be), paint pigment waste (Pb, Cr), auto-exhaust emissions (Pb, Te) etc.

1. Introduction

Reactive oxygen species (ROS) is a collective term used for oxygen containing free radicals, depending on their reactivity and oxidizing ability. They participate in a variety of chemical reactions with biomolecule (Kunwar et al.,2011).These free radicals include superoxide anions (O2-), hydroxyl (OH-), peroxyl (ROO), peroxynitrite (ONOO) and nitric oxide (NO) radicals; produced through oxidative process within the body. They can damage both the structure and function of a cell membrane in a chain reaction leading to degenerative disease conditions such as alzheimer, ageing process, cataracts, acute liver toxicity, cardiovascular disease, arteriosclerosis, nephritis, diabetes mellitus, rheumatism, inflammation process and DNA damage that can lead to carcinogenesis (Ghosh et al., 2011).

An antioxidant is a molecule capable of inhibiting or preventing the oxidation of molecules. Antioxidants are substances that may protect cells from the damaging effects of oxygen radicals, highly reactive chemicals that play a part in atherosclerosis, some forms of cancer and reperfusion injuries. Medicinal plants are an important source of antioxidants. The secondary metabolites like phenolics and flavonoids from plants have been reported to be potent free radical scavengers. They are found in all parts of plants such as leaves, fruits, seeds, roots and bark (Chanda and Dave, 2009).