eChapters

Van-Gieson Stain for Collagen Fibers
Tissues fix in 10% neutral buffered formalin and process for routine paraffin sections
Protocol
1) Deparaffinize sections and hydrate to water through descending grades of alcohol.
2) Remove mercury crystals if tissue fixed in Zenker’s or Helly’s fluid.
3) Stain in iron haemotoxylin solution for 2 to 3 minutes.
4) Wash in running water for 10-15 minutes.
5) Rinse in distilled water.
6) Counter stain in Van Gieson’ssolution for 2-3 minutes.
7) Two to three dips in 96% alcohol.
8) Dehydrate through absolute alcohol and clear in xylene.
9) Mount with D.P.X mountant.
Observations:
Red : Collagen fibers
Yellow : Muscles, RBC and cornified epithelium
Blue : Nuclei

Abstract

Entrepreneurship essentially involves economic and dynamic activities, risk- assuming and idea generation that help in developing, organising and managing a business opportunity for profits. Entrepreneurship in agriculture is not merely confined to farm activities but also involves activities interlinked and interdependent with core and peripheral agricultural activities. Impediments in the pathway of agricultural entrepreneurship in West Bengal are characterised by fragmented landholding, low financial resources and thereby debt stickiness & dependence on short-term agricultural loan. Though disbursements of short-term credit have increased substantially, the rate of increase is low. For most farmers, insurance for financial or crop crisis is unheard or have hardly any implication. The paper tries to explain the performance of various agricultural insurance schemes in terms of coverage and the benefits being delivered in India and in West Bengal. The paper analyses whether the recipients consider the schemes as an effective risk mitigation tool or merely a habit of credit-dependence in West Bengal through a diffusion model. The paper concludes with the focus on possible entrepreneurial opportunities that may offer more customised agri-insurance policies, where regional disparities could be considered and individual interests be given little more importance.

Vital Statistics

It is defined as that branch of biometry which deals with data and the laws of human mortality, morbidity and demography. According to N.B. Ryder, they “provide cumulative summaries for successive time periods of population movements like birth, death, migration, marriage and marital dissolution as well as demographic and other relevant characteristics of the individuals involved in these events.”

Measures of Vital Statistics of Population

The total number of vital events such as births and deaths are converted into rates and ratios for comparison of vital statistics from place to place or year to year. In order to determine the population at any time ‘t’ after the census or between two censuses, a number of methods have been devised. Therefore, a suitable method which makes use of births, deaths and migration statistics, if we assume that:

(i) The census data gives us the total size of the population of a region or community together with age and sex distribution

(ii) The birth, death and migration statistics during different periods are obtained from registers.

2.1. INTRODUCTION

The purpose of collecting data is to collect evidence for reaching a sound and clear solution to a problem. In this chapter we will study the sources of data, mode of data collection and its techniques.

Sources and Types of Data

Statistical data can be obtained from two sources:

Primary and

Secondary

ABSTRACT

During the efficient genetic transformation of plants with the gene of interest, some selectable marker genes are also used in order to identify the transgenic plant cells or tissues. Antibiotic and herbicide selective agents and their resistance gene are commonly used to introduce economically valuable genes into crop plants. According to the biosafety authority and consumer view points, the presence of selectable marker genes in released transgenic crops may be transferred to weeds or pathogenic microorganism in the gastrointestinal tract or soil, and will make them resistant to treatment with herbicides or antibiotics, respectively. Sexual crossing also raises the problem of transgene expression because redundancy of transgenes in the genome may trigger homology-dependent gene silencing. The future potential of transgenic technologies for crop improvement depends greatly on our abilities to engineer stable expression of multiple transgenic traits in a predictable fashion and to prevent the transfer of undesirable transgenic material to non-transgenic crops and related species. Therefore the development of efficient marker free transgenic plants system is very essential. That is why many technologies and various approaches designed to timely elimination of transgenes when their function is no longer needed. Due to the limited number of available selectable marker genes, in future the stacking of transgenes will be increasingly desirable. The production of marker-free transgenic plants is now a critical requisite for their commercial deployment and also for engineering multiple and complex trait. Here we describe the current technologies to eliminate the selectable marker genes (SMG) in order to develop marker-free transgenic plants and also discuss the regulation and biosafety concern of genetically modified (GM) crops.

Tillage

The mechanical manipulation of soil with tools and implements for obtaining conditions ideal for seed germination, seedling establishment and growth of crops is known as tillage. Tillage may be described as the practice of modifying the state of the soil in order to provide conditions favourable to crop growth.

Programme planning and implementation constitute the core operational dimensions of community mobilization that translate vision, analysis, and community aspirations into concrete actions and systematic interventions capable of achieving meaningful and sustainable development outcomes. The transformation of community needs and opportunities into well-designed programmes requires sophisticated planning methodologies that can navigate complex social, economic, and political environments while building local ownership and capacity for long-term success and sustainability. Effective programme planning represents both an art and a science that combines analytical rigor with cultural sensitivity, technical expertise with participatory approaches, and strategic thinking with practical implementation considerations that must be balanced throughout the planning and execution process. The quality of programme planning significantly

Introduction

Growth enhancement technologies have been widely embraced in the livestock industry to improve growth, efficiency and carcass traits. For over 50 years, estrogenic and androgenic hormone implants were widely used in the cattle feeding industry, and it is estimated that approximately 97 percent of feedlot cattle in the U.S. receive one or more implants during the finishing phase (Barham et al., 2003; Tatum, 2006). There is a large body of literature that indicates the positive growth enhancement effects which implants impart on growing and finishing cattle and producers have extensively used implants to increase live body weight, improve average daily gain and feed efficiency, and reduce the number of days cattle are on feed (Apple et al., 1991; Duckett et al., 1997;Milton and Horton, 1996; Perry et al., 1991). Likewise, there is a growing body of literature that indicates the positive response on growth and carcass characteristics that finishing cattle have to beta-adrenergic agonists. The hog industry, and more recently, the cattle feeding industry have increased utilization of beta-adrenergic agonists to improve the efficiency of production. While the use of Ractopamine hydrochloride (Paylean^®, Elanco Animal Health, Greenfield, Indiana) has been used extensively in hog production since its approval by the Food and Drug Administration (FDA) in 1999, similar pharmaceutical feed additives have only recently been implemented in commercial cattle feeding operations. Beta-adrenergic agonist use in fed cattle has substantially increased following the FDA approval of Ractopamine hydrochloride (Optaflexx^®, Elanco Animal Health, Greenfield, Indiana) in 2003, and the approval of Zilpaterol hydrochloride (Zilmax^®, Merck Animal Health, Summit, New Jersey) in 2006. The use of both Ractopamine hydrochloride (RH) and Zilpaterol hydrochloride (ZH) have shown to elicit similar improvements in live weight gain, average daily gain, and feed efficiency as those reported in hormone implants (Allen et al., 2009; Avendano-Reyes et al., 2006; Beckett et al., 2009; Elam et al., 2009; Gruber et al., 2007; Kellermeier et al., 2009; Scramlin et al., 2010). In addition, these compounds have been reported to have profound effects on hot carcass weight, Longissimus muscle area and carcass cutability (Gruber et al., 2007; Hilton et al., 2010; Rathmann et al., 2009; Scramlin et al., 2010; Shook et al., 2009; Vogel et al., 2009). It is because of this that cattle feeders have drastically increased use of beta-agonists since their FDA approval, and a growing portion of the fed cattle population receive a dietary beta-agonist supplement that is provided within the final 20 to 30 days of finishing.

Agricultural production has made rapid strides in the past four to five decades and has enabled India to achieve self-sufficiency in food grain production. This was only through ìGreen Revolutionî which ushered during 1967-68 onwards, when use of old agricultural practices were mostly done away with. Neglect of old or indigenous agricultural practices such as use of organic manures (farm yard manure - FYM, compost, green manure), cultural control methods like sanitation, tillage/removal of weeds, adjusting sowing/harvesting time crop rotation, trap crops, water and fertilizer management and host plant resistance, and use of plant based insecticides like neem or nim (Azadirachta indica), drek (Azadirachta indica), nirgudhi or bana (Vitex negundo) vis-a-vis biological control measures, has resulted in outbreak of ill-effects in human beings and animals through contaminated food stuffs (Gupta, 2006a).

Biosensors are the next generation detection devices, use biological sensing elements like enzymes, nucleic acids, antibody, cells, organelles, tissues, receptors; have got varied applications in the field of drug discovery, diagnosis, biomedicine, food safety and processing, environmental monitoring, defense and security etc. Microbial biosensors are analytical device that uses naturally occurring or engineered microbial cells as the recognition elements to detect physiological changes in cells with the help of suitable physicochemical transducer.

Introduction

The soil is an invaluable resource that sustains life in innumerable ways. It provides a dynamic foundation for agriculture, supporting growing crops, and serving as a habitat for organisms. It plays a crucial role in maintaining biodiversity. Additionally, soil acts as a filter, purifying and storing water, and it reserves essential plant nutrients. however, soil resources face various threats that endanger their health and productivity. Soil erosion, the removal of the top layer of soil through natural or human-induced processes, poses a significant challenge. It not only leads to a loss of fertile soil but also causes widespread environmental degradation, and reduced agricultural productivity in response to this difficulty, soil conservation emerges as a principal priority. Soil conservation incorporates a range of practices and strategies to prevent soil erosion, and promote sustainable land use. By adopting and implementing effective soil conservation measures, we can safeguard this irreplaceable resource for future peers and ensure food security and environmental sustainability.

The main highlight of the study is to shed light on the causes and consequences of soil erosion, exploring different conservation practices and techniques; also to understand the vital processes and factors contributing to soil erosion, including wind and water erosion. Additionally, to study its impact on soil and crop production and strategies for different conservation practices to mitigate erosion, preserve soil fertility, and ensure the long-term sustainability of our agricultural systems and ecosystems.

Establishment of databases and data warehouse; knowledge discovery from database/data warehouse (KDD) and development of the mechanism of dissemination of knowledge on information networks as per requirements of user groups is gaining importance now a days. Since there is a large number of data collection agencies and equally diverse resources for which the information is collected, it is easy to visualize the heterogeneity of information. The problem is compounded by the fact that there are no common standards that are applied in data collection. Designing data warehouses to integrate the collected information poses a formidable challenge to any data warehouses architect. In order to use the information for planning and decision-making level, data have to be integrated and aggregated properly.

Introduction

Packing and transportation forms an important component in fish trade whether live fish, fish seed, fresh fish, iced fish or dried fish. There are few reasons necessitating packing and transportation.

    ·    The movement of fry/juvenile for stocking into farms or restocking into natural environment.
    ·    The movements of fish to markets for sale selling/consumption.
    ·    The movements of ornamental aquarium live fish for trade.
    ·    The movement of aquatic animals for research purpose.

Abstract

Most dryers operate at low thermal efficiency. The development of models and control systems offers an opportunity to improve dryer operation and efficiency. Dryer control is very important and with deeper understanding of the drying concept, new “smart” dryers can be made more reliable and more cost-effective than classical dryers. This chapter will provide an overview of necessity of control and safety measure in food drying system, and terminology related with drying control system. Different control strategy includes conventional and advanced techniques implemented in drying operation are reviewed  here and simple outline of intelligence control system in drying technology are briefly described in succeeding section. Various hazards in drying process and risk and safety management will also be discussed.

Synonym : Hydrophobia, Lyssa, Jalatanka

This is an acute highly fatal viral disease of all warm blooded animals including man characterized by signs of abnormal behaviour, nervous symptoms (increased excitability and irritability), impairment of consciousness, ascending paralysis and death. The disease was variously known as lytta or lyssa coming from the belief that the disease was caused by a worm under the tongue (lytta) or hydrophobia, which describes the thirst and fear of water associated with the disease. The English name rabies derived from Latin word ‘Rabere’ which has been derived from Sanskrit word ‘Rabhas’  meaning raging, furious, savage, madness or   to do violence whereas Greek term hydrophobia is now specifically used  for rabies in man. The symptom hydrophobia (fear of water) is developed due to painful spasm of muscles of deglutition.

Rice (2n = 24) is an annual grass (family: Graminae) with terminal panicles bearing the grains. It originated in Asia between the fourth and fifth millennia B.C. A substantial portion of the people in south and Southeast Asia and the southern part of China are depending on rice as staple food (Chang, 1976). Rice is planted in the most diverse environmental conditions of any major food crop, from sea level to around 3000 m altitude and from 30°S to 49°N. Temperature and day lengths are therefore quite diverse in the rice growing areas. Rice is also grown under water regimes ranging from dry land to deepwater fields where water may reach 5 m depth. The wide geographical distribution of rice and its early history of cultivation have resulted in the development of a great diversity of varietal types (Vergara, 1985).

Rice has been used as food by man for over 10,000 years and has fed a great number of people for a longer period than has any other crop. “Rice is life” for almost four billion people of the world contributing over 20 per cent of the total caloric intake of human population (Chaudhary and Tran, 2001). Rice is cultivated in 113 countries and on all continents except Antarctica and it is the staple food for 17 countries in Asia and the pacific, eight countries in Africa, seven countries, in Latin America and the Caribbean and one in the near east (IRC, 2003).

Globally rice is grown in around 155 million ha occupying one tenth of arable land, with an annual production of 579 million tonnes of rough rice (Iiyas Ahmed, 2003). The average productivity of 3.89 t ha^-1 of rice provides a per capita. Consumption of 100 to 240 kg per year across the globe. Over 90 per cent of the world’s rice is produced and consumed in Asia and more than half of it from India and China exploiting maximum arable land. More than two billion people obtain their 80 per cent calorie from rice and its derived products from 90 per cent of world’s rice area (FAO, 1999). Rice based on production systems and their associated post harvest operations employ nearly a billion people in rural areas in developing countries (IRC, 2003).

Abstract

Microbes are key ingredients of various ecosystems and backbone of modern biotechnology and industry. In addition to their cultivation, purification and characterization, preservation of microorganisms is equally important. Different preservation methods and protocols have been developed for long term preservation of microbes. Microbial Resource Centers (MRCs) are providing active services in the field of life sciences. National Centre for Microbial Resource (NCMR) at National center for Cell Science (NCCS), Pune is one of the MRCs. Unlike aerobes little work has been done in the area of cultivation and preservation of anaerobic microbes. In the previous chapter we have discussed the technicalities and problems of anaerobic cultivation. The aim of current chapter is to provide the recent overview of preservation of anaerobic microorganisms like methanogens, sulfate and metal reducers and the obligate anaerobes of clinical and industrial importance with fermentative mode of life style.

Water is precious, but it is a finite natural resource. Although it accounts for about three-fourth of the earthís surface yet very small fraction of it i.e., only 2.7 per cent of the global water is available as fresh water. Around 2.8 billion people live in areas of high water scarcity (Anonymous, 2014d). Out of the total amount of the fresh available water merely 30 per cent is withdrawn to meet the water requirements of the human and livestock population while the remaining 70 per cent is required to produce food crops that fuel human activities.

4.1. Digestive System of Rabbit

The digestive system of rabbit includes:
    A.    The digestive tract 
    B.    Its associated glands
The digestive system breaks down the complex molecules into the simpler one so that it is readily absorbed from gastro intestinal mucosa.
 
A. The digestive tract (gastro intestinal tract/ alimentary canal)

The digestive tract is essentially a tubular structure for the ingestion and digestion of food. In an adult (4 to 4.5 kg) or semi-adult (2.5 to 3 kg) rabbit the total length of the alimentary canal is 4.5 to 5 m. It commences from mouth and terminates in anus. G.I.T is divided into following parts:

The Cucurbitaceae family consists of nearly 100 genera and over 750 species. Although most of them have Old World origin (Whitaker and Davis, 1962), many species originated in the New World and at least seven genera have origins in both hemispheres (Esquinas-Alcazar and Gulick, 1983). There is considerable genetic diversity within the family and the range of adaptation for cucurbit species includes tropical and subtropical regions, arid deserts and temperate locations. A few species are adaptable to production at elevations as high as 2000 m. The genetic diversity in cucurbits extends to both vegetative and reproductive characteristics. There is considerable range in the monoploid (x) chromosome number (Jeffrey, 1990) including 7 (Cucumis sativus), 11 (Citrullus spp., Momordica spp., Lagenaria spp., Sechium spp., and Trichosanthes spp.), 12 (Benincasa hispida, Coccinia spp., Cucumis spp. other than C. sativus, and Praecitrullus fistulosus), 13 (Luffa spp.), and 20 (Cucurbita spp.).
 
Archaeological evidence indicated that cucurbits were present in ancient and prehistoric cultures. Lagenaria was associated with man as early as 12,000 BC in Peru (Esquinas-Alcazar and Gulick, 1983). Archaeological expeditions in the Oaxaca region of Mexico have reported that Cucurbita pepo was associated with man as early as 8500 BC and cultivated by 4050 BC (Esquinas-Alcazar and Gulick, 1983). Written Chinese records describing the use of cultivated cucurbits have been found as early as 685 BC (Herklots, 1972).