eChapters

Introduction
 
The future of functional foods is bright and there are several reasons for the growing interest in “functional” foods, such as awareness of personal health deterioration, led by busy lifestyles with poor choices of convenience foods and insufficient exercise; increased incidence of self-medication; increased level of information from health authorities and media on nutrition and the link between diet and health; scientific developments in nutrition research; and a crowded and competitive food market, characterized by pressurized margins. Now, the consumer is well aware of the relationship between diet and health. Consumers understand that the risk for chronic diseases such as heart disease, stroke, colon cancer, and type II diabetes is possibly preventable by modification in their diet pattern. This scientific progress in understanding the relationship of diet to disease, along with increasing health care costs and the consumer’s desire to initiate healthy eating and lifestyle habits, makes this a potentially fertile ground for the development of food products that meet these demands.

In most countries there is no legislative definition of the term drawing a border line between conventional and functional foods is challenging even for nutrition and food experts. Functional foods do not have a specific place in food law; consequently there is a great deal of confusion about what they are, could or should be. A common perception is that functional foods are foodstuffs either with one or more specific added ingredients (components), or certain substances of common ingredients (components) removed and which are claimed to have a positive influence on human health or well being. The term ‘functional foods’ for this group of products is considered to be an unfortunate one since all foodstuffs are or should be functional and a more appropriate term might be helpful.

For the laboratory diagnosis of the parasitic infections, it is essential to examine various excretory-secretory products, viz., faeces, urine and nasal discharges as  examination of such material is essential for clinical diagnosis. It is extremely rare to get an animal completely free from parasites and at the same time all animals may not show signs of disease. Since, most of the important helminths and coccidia inhabit intestinal tract, therefore, clinical diagnosis of gut dwelling helminths and coccidia depends mainly on identification of ova/oocyst in faecal samples.

Examination of faecal sample for helminthic infections

Gross Examination: It precedes the microscopic examination of faecal sample and includes collection of information as below:
    a)    Consistency: Condition of the faeces should be noted as soft, watery (diarrhoeic) or hard but it should be kept in mind that the description varies with the concerned animal species.
    b)    Colour: It is to report the unusual faecal colour, if any as mud coloured foul  smelling faeces in calves indicate ascarid infection; tarry coloured faeces in dog is pinpointing of hookworm infection.

Mange or parasitic dermatitis is caused by the infestation of mange mites e.g. Sarcoptes, Psoroptes, Demodex spp. and a range of other mites. The condition is characterized by itching, rubbing of the infested parts of the body against hard objects. Lesions include hair loss, dry, rough, thickened and wrinkled skin accompanied by the presence of scab and foul odour in some cases. Some mites burrow into the host epidermis (Sarcoptes) while others spend their entire life cycle on the skin surface (Psoroptes). Most mites are microscopic or less than 1mm in length; therefore skin scrapings are taken from the affected area of animals suspected to be suffering from mange or parasitic dermatitis.

Collection of blood

Blood sample should be collected in a capped clean and dry vial containing an appropriate amount of anticoagulant. The commonly used anticoagulants are ethylene diamine tetra acetic acid (EDTA) @ 1-2 mg/ml and heparin @ 5 I.U./ml of blood.

HELMINTHS 
Collection of parasites

Fresh helminth parasites should be collected, as far as possible to achieve better results in staining. This can best be done by conducting a post­mortem examination of the animal for which the procedure given below should be followed. On opening the body and before removal of the viscera, examine the body cavity (for free lying parasites, e.g. Setaria), subcutaneous tissue (for Parafilaria and Hypoderma larvae) and the surfaces of internal organs for spot or nodules that might contain parasites. Remove them for histopathological examination (in 10 % formalin). Remove the visceral organs separately and place them in different sized enamel trays.

Diagnosis of parasitic infections is routinely done with conventional parasitological techniques as described earlier. However, when the parasitaemia is too low to be detected by the conventional methods as in subclinical infections and/or carrier animals advanced diagnostic tools are used for the early, sensitive as well as specific diagnosis.

Serological techniques

Diagnostic techniques used mostly in laboratories need various reagents and equipments to perform the test. However, a diagnostic kit is the extrapolation of the same method by packing it in a user friendly format, accompanied by all or most of the reagents required to perform the test and can also be done outside the laboratory or under field conditions.

Synonyms : Aphthous fever; Contagious aptha;  Panzootic aptha; Vesicular aptha.

Foot and mouth disease (FMD) is an acute, highly communicable viral disease affecting cloven hoofed animals, both domesticated and wild. It is the most contagious disease known in the animal kingdom. It is characterized by vesicular eruptions in the epithelium of the buccal cavity, tongue, nares, muzzle, feet, teats and udder.

Synonyms : Hog eholera, European swine fever

It is an acute highly infectious viral septicaemia of swine of all ages characterized by rapid and sudden onset, high morbidity and mortality and generalized  haemorrhages. A chronic course  followed by recovery after treatment has been reported in older pigs and appearance of new born pigs with congenital defects.

Etiology : The etiological agent causing hog cholera is a linear  single stranded positive sense RNA virus under the genus Pestivirus and family Flaviviridae. The virions are spherical, 40-50 nm in diameter  and consist of a tightly adherent lipid bilayer envelope covered with glycoprotein peplomers surrounding an icosahedral capsid of 28-35 nm in diameter. The genome is 12 kb in length with 5’end capped but  3’end  not  polyadenylated. The genes for structural proteins are located at 5’ end of genome. The virus replicates in the cytoplasm and matures within cytoplasmic vesicles. Though there is only one antigenic type of the virus, a number of strains of variable virulence and antigenicity has been reported. The causative agent was first thought to be a gram negative bacterium named Salmonella cholera suis. Later, it was confirmed that the disease is caused by a  virus.

Synonym : Hydrophobia, Lyssa, Jalatanka

This is an acute highly fatal viral disease of all warm blooded animals including man characterized by signs of abnormal behaviour, nervous symptoms (increased excitability and irritability), impairment of consciousness, ascending paralysis and death. The disease was variously known as lytta or lyssa coming from the belief that the disease was caused by a worm under the tongue (lytta) or hydrophobia, which describes the thirst and fear of water associated with the disease. The English name rabies derived from Latin word ‘Rabere’ which has been derived from Sanskrit word ‘Rabhas’  meaning raging, furious, savage, madness or   to do violence whereas Greek term hydrophobia is now specifically used  for rabies in man. The symptom hydrophobia (fear of water) is developed due to painful spasm of muscles of deglutition.

Synnoyms : Pseudorinderpest, Goat plaque, Goat catarrhal fever

It is a severe rinderpest like disease of small ruminants viz., sheep, goat and wild ruminants characterized by pyrexia, catarrhal inflammation of ocular and nasal mucous membrane, erosive stomatitis, enteritis and pneumonia. The disease is also called goat plaque, kata, pseudorinderpest and  stomatitis pneumo-enteritis complex. The current and standard name of the disease was chosen by the International Workshop of the disease in Ibadan, Nigeria, 1980.

Synonyms : Variola capra

It is a malignant disease of goats characterized by high rise of body temperature and generalized pock lesions.

Distribution : Goat pox has been reported from all parts of the world. Various workers have reported the  outbreaks of the disease from different states of India. It is widely prevalent in W.B. and may attain epidemic proportion with mortality rate of 80% in kid and ~50% in adults.

Synonyms : Variola ovine, ovine pox

Sheep pox causes a severe and highly contagious disease in sheep which is listed in group A disease of the OIE. It is defined as a malignant pox of sheep characterized by fever and generalized development of pock lesions. Lesions first appear as vesicles which later turn into pustules on the exposed parts of the body. There is high mortality rate in lambs.

Distribution :  The disease is in existence since very long in Asia and Europe. It is prevalent in many areas of middle East countries, Africa, Asia and  Mediterranean countries. There are lot of disease outbreaks in India around the year. European Union with the exception of occasional sporadic outbreak in eastern areas of Greece, U.S.A. and Australia are free from the disease.

Synonyms : Catarrhal fever, Sore mouth, Epizootic catarrh, Sore muzzle, Pseudo foot and mouth disease, Malarial catarrhal fever.

It is an infectious, noncontagious arthropod borne viral disease of domestic and wild ruminants namely sheep, goat , cattle, camels, llamas, deer and antelopes. This is predominantly a disease of sheep but occasionally cattle and  goats are subclinically affected. The disease is characterized by high fever, catarrhal stomatitis, rhinitis, enteritis and lameness due to inflammation of the coronary bands and sensitive laminae of the feet. There are at least  25 serotypes of bluetongue virus worldwide within the BTV serogroup. Genetic variability occurs within a group due to genetic drift of individual gene segments and genetic reassortment when ruminants or other vectors are infected with more than one strain.

Synonyms :  Viral bovi. rhinotracheitis, Red nose, I.B.R, Infectious pustular vulvovaginits (IPV), Coital exanthema, Infectious pustular balanoposthitis (IPB), Bovine herpes virus 1(BHV 1).

It is a highly infectious disease of bovine characterized by high fever, rhinotracheitis, conjunctivitis, with a short course and high recovery rate. Encephalitis, the systemic form of the disease in new born calves, infectious pustular vulvovaginits/balanoposthitis are other syndromes caused by the same virus. Genetic isolates of various clinical isolates has found subtypes of BHV 1; a respiratory subtype BHV 1.1, a genital subtype BHV1.2 and an encephalatic subtype BHV 1.3, which is now termed as BHV 5.

Synonyms :  Three days sickness, Stiff  sickness,  Dengu fever of cattle, Bovine epizootic fever and Lazy man’s disease.

Bovine ephemeral fever (BEF) is a viral disease of cattle, Bos Taurus, Bos indicus and Bos javanicus and water buffalo Bubalus bubalis, although  subclinically infect a greater range of ruminant species. Although the disease has a variety of names, bovine ephemeral fever is most commonly used  and is very apt. The disease in cattle is characterized  by acute febrile reaction, stiffness, lameness and spontaneous recovery in three days.  The morbidity may be high but the mortality is low. It mainly occurs in subtropical and temperate regions of Africa, Asia and Australia.

Canine parvovirus 2 (CPV-2), the causative agent of  acute haemorrhagic enteritis and myocarditis in dogs, is one of the most important  pathogenic viruses. It is a highly contagious  and often fatal disease.  CPV-2 was first recognized in 1977 and since then it has been well established as an enteric pathogen of dogs throughout the world causing  high morbidity (100%) and frequent mortality up to 10%.   The disease is characterized by 2 prominent clinical forms (i)  enteritis with vomition and diarrhea in dogs of all ages (ii) myocarditis and subsequent heart failure in pups of less than  3 months of age. The virus was named CPV-2 in order to differentiate it from a closely related parvovirus of canine known as CPV-1 or minute  virus of canine (MVC). MVC, a completely different parvovirus, had not been associated with   natural disease until 1992.

Synonyms : Black leg, quarter ill
This is an acute infectious but non contagious disease of cattle, goat, sheep, swine and deer  caused by Cl. chauvoei characterized by development of focal gangrenous and emphysematous myositis. This gives rise to crepitation and serosanguinous swelling in heavy muscles especially in gluteal muscles and associated with severe toxaemia and high mortality.

Synonymous  :  Pearl’s disease, scrofula

It is a chronic insidious contagious disease of man and animals caused by  tubercle bacilli characterized by development of tubercles in lung tissue, lymph nodes or other organs resulting in caseation and calcification. The tubercles are frequently located in lungs and lymph nodes. But they may be formed in the liver, spleen, intestine, peritoneum, meninges and long bones.

Synonyms : Bang’s disease, Contagious abortion, Infectious abortion, Enzootic abortion, Undulant fever, Malta fever, Mediterranean fever

Brucellosis, one of the most ancient diseases,  continues to be a problem for humans and animals throughout the world causing enormous economic losses and significant human morbidity in endemic areas. The bacterial pathogen is classified by the CDC as a category B pathogen that has the potential for development as a bioweapon. It is one of the important zoonotic disease and considered  as the most common laboratory acquired pathogen with >5 lakh human cases reported  annually  around the world. Brucellosis is a chronic infection which can result in infertility, delayed heat, interrupted lactation, loss of calves, wool, meat and milk production in animals and undulant fever in humans.

Synonyms : Farcy, Pacin, Carn

Glanders is a contagious, acute or chronic, usually fatal anthropo-zoonotic disease of Equidae caused by Burkholderia mallei and characterized by serial development of ulcerating nodules that are most commonly found in the upper respiratory tract, lungs, and skin. The first recorded description of glanders is from the third century from Aristotle. In 1664, glanders was recognized as a contagious disease and in 1830, it’s zoonotic potential was suspected. In the late 1800’s, the Mallein test (1891) was developed for diagnosis of glanders. By 1910, the U.S., Canada and Great Britain all implemented glanders-control programs. B. mallei was eradicated from the United States in 1929.

Synonyms : Pasteurellosis, shipping fever, Stockyardis disease, Stockyardis pneumonia, Barbone disease

Hemorrhagic septicaemia is a particular form of pasteurellosis caused by Pasteurella multocida and manifested by an acute and highly fatal septicaemia mainly in  cattle and water buffaloes. The disease includes pneumonic pasteurellosis (shipping or transport fever), a disease caused mainly by P. haemolytica, although various serotypes of P. multocida are occasionally involved. Although the morbidity of pneumonic pasteurellosis of cattle can be high, the mortality rate is much less than that of hemorrhagic septicemia.

Synonyms :  Weil’s disease, Stuttgart disease, Infectious jaundice, Haemoglobinuria, Rice field worker’s disease, Swine handler’s disease, Sugarcane worker’s disease, Canine typhus

Leptospirosis is a worldwide zoonosis. According to the occupational groups involved and the nature of the disease presentations, different names have been used, e.g. seven-day fever found commonly in Japan, Cane cutter’s disease in Australia, Rice field Leptospirosis in Indonesia and Fort Bragg fever, which appeared as an outbreak in the US. Weil’s disease, which is one of the severe forms of this disease, occurs in many countries, including India and other South-East Asian Countries, China, continental Europe and England. Leptospirosis exists in all the five inhabited continents and in a large number of countries. It occurs in tropical, subtropical and temperate zones.  Leptospirosis is also an important cause of abortions, stillbirths and reproductive failures in cattle and pigs.  Periodic ophthalmia in horse is commonly associated with leptospiral infections. Leptospirosis was first recognized in humans in 1886 by Weil and characterized by icterus.  Leptospira have been isolated from a variety of animal species and humans manifesting various clinical syndromes such as mud fever and swine herd disease.

Synonyms : Infectious adenitis, Equine distemper
It is  an acute infectious disease of equines characterized by catarrhal inflammation of the upper respiratory tract with suppuration and abscessation of the associated lymph node.
 
Etiology :  Strepococcus  equi subspecies equi is a Gm+ cocci that produces a -b haemolysin characterized by clear haemolysis surrounding colonies growing on blood agar. It is a biovar or genovar of S. zooepidemicus. Equines are very much susceptible and different genetic clones occur geographically. S. equi varies in virulence which is related to M protein and hyaluronic capsule produced. S. equi variant without capsule causes mild disease.

Synonyms :  Splenic fever, wool sorter’s disease, Tarka
It is an  infectious disease of livestock and worldwide in distribution. This is a zoonotic disease. It is also  known as splenic fever because of enlargement of the spleen (splenomegaly).

Etiology : It is caused by a rod shaped bacteria of 4-8 µm X 1-1.2 µm known as Bacillus anthracis. It is non-motile, capsulated, aerobic, spore bearing and Gm+  in nature. The organisms  remain in chains. It was the first pathogenic bacterium to be observed under the microscope, the first communicable disease to be transmitted by inoculation of infected blood, the first bacillus to be isolated in pure culture and shown to possess spores and the first bacterium used for  preparation of an attenuated vaccine.

Synonyms : Johne’s disease, Chronic  specific enteritis of cattle, Chronic bacillary dysentery
 
Paratuberculosis (Johne’s disease), an infectious disease caused by Mycobacterium avium subspecies paratuberculosis (MAP), has been recognized as a major disease of ruminants for over a century. It is primarily a disease of domestic and wild ruminants and causes heavy economic losses  in cattle, sheep and goat industry. It is spreading insidiously among animal population worldwide.  Recently it has been shown  that  the disease is not restricted to ruminants only and the host range is expanding to cause disease in other species including Crohn’s disease in humans. In India, the  disease has been occurring  since early 1930s in cattle, sheep and goats in almost every state. The disease in ruminants is clinically characterized by chronic intermittent profuse diarrhea, weight loss, emaciation and finally death.

(+)sense RNA (plus-sense RNA)     :    A virus with a single-stranded RNA genome of the same polarity (‘sense’) as mrna.

B    :    Born.     
B cell, also known as a B lymphocyte      :    One of the major types of cells in the immune system. B cells can differentiate to form memory cells or antibody-forming cells. OR A type of lymphocyte derived from bone marrow stem cells that matures into an immunologically competent cell under the influence of the bursa of Fabricius in the chicken and bone marrow in nonavian species. Following interaction with antigen, it becomes a plasma cell, which synthesizes and secretes antibody molecules involved in humoral immunity.

C    :    Cytosine.
C domains    :    The constant regions of the polypeptide chains of immunoglobulin molecules are made up of one or more constant domains or C domains of similar structure; each immunoglobulin chain also has a single variable or V domain.

D gene segments, diversity gene segments     :    D gene segments, or diversity gene segments, are short DNA sequences that join the V and J gene segments in rearranged immunoglobulin heavy-chain genes and in T-cell receptor â and ä chain genes. See gene segments.    
D loop    :    An extended loop of single-stranded DNA displaced from a duplex structure by an oligonucleotide.

E    :    Reduction potential.    
Early induced responses    :    The early induced responses or early nonadaptive responses are a series of host defense responses that are triggered by infectious agents early in infection. They are distinct from innate immunity because there is an inductive phase, and from adaptive immunity in that they do not operate by clonal selection of rare antigen-specific lymphocytes.

F    :    Faraday constant. OR Food - represents BOD in the F/M ratio. Expressed in pounds.

F factor    :    The fertility factor, a plasmid that carries the genes for bacterial conjugation and makes its E. coli host cell the gene donor during conjugation. OR A large bacterial plasmid, known as the sex-factor plasmid because it permits mating between F+ and F- bacteria. 

G    :    Gibbs free energy OR Guanine.

G proteins    :    G proteins are intracellular proteins that bind GTP and convert it to GDP in the process of cell signal transduction. There are two kinds of G protein, the heterotrimeric (α,β, γ) receptor-associated G proteins, and the small G proteins, such as Ras and Raf, that act downstream of many transmembrane signaling events.

H    :    Hour.

H    :    Planck’s constant.

H antigens, histocompatibility antigens   :    H antigens or histocompatibility antigens, are known as major histocompatibility antigens when they encode molecules that present foreign peptides to T cells and as minor H antigens when they present polymorphic self peptides to T cells. See also histocompatibility.

I&D    :    Incision and drainage.     
I&E    :    Internal and external.     
I&O    :    Intake and output.     
I.P. (IP)    :    Intraperitoneal .    
I.V. (IV)    :    Intravenous.

J    :    Joule.

J    :    Joining segment.

J chain      :    A polypeptide present in polymeric IgM and IgA that links the subunits together.
    
J gene segments, joining gene segments     :    The J gene segments, or joining gene segments, are found some distance 5' to the C genes in immunoglobulin and T-cell receptor loci. A V and in the case of IgH chains, tcrâ chains and tcrä chains, a D gene segment must also rearrange to a J gene segment to form a complete V-region exon.

K    :    Kilo (10³g).
 
K    :    Potassium.

KA    :    Ketoacidosis.

Karlvon Baer    :    Person who‚ in 1828‚ published on the developmental stages in mammalian eggs and showed that an undifferentiated‚ single-celled egg grows into a many-celled embryo.

K    :    Kilo (10³g).

K    :    Potassium.

KA    :    Ketoacidosis.

Karlvon Baer    :    Person who‚ in 1828‚ published on the developmental stages in mammalian eggs and showed that an undifferentiated‚ single-celled egg grows into a many-celled embryo.

M    :    Milli (10^-3l).

M    :    Molar (mol/L).

M    :    Microorganisms - small organisms which require a microscope to be seen. M represents the SS in the mixed liquor and is part of the F/M ratio. 

N    :    Avogadro’s number.

N    :    Nano (10^-9m).

N&M    :    Nerves and muscles.
   
N&T    :    Nose and throat.

N&V    :    Nausea and vomiting.
    
NA    :    Nucleic acid.

O    :    Pint.

O antigen      :    A polysaccharide antigen extending from the outer membrane of some gram-negative bacterial cell walls; it is part of the lipopolysaccharide.

O&E    :    Observation and examination.

Oa    :    Open access.

P    :    Phosphate.

p    :    Pico (10^-12).

P&S    :    Permanent and stationary.

PO    :    Per oral; by mouth (see also IV; IP;).

PA    :    Postero-anterior; pulmonary artery.
   
PAC    :    P1-derived artificial chromosome.

Q    :    Ubiquinone (coq).

Q    :    Every.

Q fever      :    An acute zoonotic disease caused by the rickettsia Coxiella burnetii.

R    :    Gas constant.

R factors  R plasmids      :    Plasmids bearing one or more drug resistant genes.

R group    :    Shorthand for the side chain of an amino acid. 

S    :    Second.

S    :    Sedimentation coefficient. OR Svedberg unit.

S phase    :    The period during the cell cycle when the chromosome is replicated.

S7P    :    Sedoheptulose-7-phosphate.

T    :    Thymine

T    :    TH1 cells are a subset of CD4 T cells that are characterized by the cytokines they produce. They are mainly involved in activating macrophages, and are sometimes called inflammatory CD4 T cells. OR TH2 cells are a subset of CD4 T cells that are characterized by the cytokines they produce. They are mainly involved in stimulating B cells to produce antibody, and are often called helper CD4 T cells. OR The term TH3 cell has been used to describe unique cells that produce mainly transforming growth factor-â in response to antigen; they develop predominantly in the mucosal immune response to antigens that are presented orally. OR See regulatory T cells.

U    :    Units.

U&L    :    Upper and lower.

U&MNP    :    Ulnar and median nerve palsy.

U/O    :    Under observation for.
  
U/WB    :    Unit of whole blood.

UA    :    Umbilical artery; uric acid; urinalysis.
   
UC    :    Ulcerative colitis; unchanged; unconscious. 

V    :    Volt.

V domain    :    The variable region of the polypeptide chains of an immunoglobulin or T-cell receptor is composed of a single N-terminal V domain. Paired V domains form the antigen-binding sites of immunoglobulins and T-cell receptors.

W    :    Watt.

W&D    :    Warm and dry.

W/A    :    Weakness atrophy.
    
W/C    :    Wheelchair.
    
W/E    :    Wound of entry.
    
W/U    :    Workup.