eChapters

In sub-tropical agro-climatic conditions of India, a variety of insect-pest, weeds and diseases hamper the economic growth of field crops. For effective control of these pests, a number of pesticides including insecticides, fungicides, herbicides and nematicides are to be applied. Estimation technique for single pesticideonly can not be followed for detection of residues of all categories of pesticides. Hence, this constantly expanding use of pesticides on food crops accentuates the need for rapid, precise and sensitive method for determination of pesticide residues of all the major groups of pesticides. In such situation, multi-residue analytical technique can be efficiently followed for detection and estimation of multiresidues of intra and inter class xenobiotics.

The purpose of multiresidue analysesistodeterminetheresidues ofas manypesticides as possible withina short period of time even if the recoveries of somecompounds are low. In multiresidue methods the recoveries up to 70% are accepted.The recoveriesless than70%have tobe mentionedspecifically. Multiresidue analysis is relativelymore sensitiveand requires extreme vigilance on the part of the analyst during processingof samples. Additionally, instrument, GLC,need to beequipped withcapillary columnsforhigh resolutionand sophisticated detectorslikeECD, NPDand MSD for detectingmulti-residues of the pesticides. Multiresidue analytical methods/techniques havebeen developed for determination of general pesticide residues for some environmental componentsand differentfood commodities.Criteria formulti-pesticides screening as per California Department of Food and Agriculture (CDFA) are:

7.1 Introduction
In chapter 5, the cluster sampling has been discussed in which clusters were considered as sampling units and all the elements in the selected clusters were enumerated completely. It has been stated there that the cluster sampling is economical under certain circumstance but the method restricts the spread of sample over the population which results generally in increased sampling variance of the estimator under consideration. It is, therefore, logical to expect that the efficiency of the estimator will be increased by distributing the elements over a large numbers of clusters. Hence instead of observing all the units in the selected clusters, one may like to observe only few elements of the selected clusters. This will involve the sampling work to be done also within the selected clusters. If we do so, the process of selection will involve sampling work to be done at two stage, one for selecting the clusters where sampling units are the clusters and the second for selecting the units (elements) within the selected clusters where sampling units are the elements.
The process of sampling can be done at more than two stages. In this case the first stage unit will be larger than the second stage units and the second stage units will be larger than the third stage units and so on. Due to process of sampling in stages, such sampling is called “Multistage sampling.”

Q.1 Cluster sampling restricts the spread of sample over the population which results generally in increased sampling variance of estimators under considerataion.

Q.2 In adopting cluster sampling, few selected clusters are enumerated partially.

7.1 Introduction

In chapter 5, the cluster sampling has been discussed in which clusters were considered as sampling units and all the elements in the selected clusters were enumerated completely. It has been stated there that the cluster sampling is economical under certain circumstance but the method restricts the spread of sample over the population which results generally in increased sampling variance of the estimator under consideration. It is, therefore, logical to expect that the efficiency of the estimator will be increased by distributing the elements over a large numbers of clusters. Hence instead of observing all the units in the selected clusters, one may like to observe only few elements of the selected clusters. This will involve the sampling work to be done also within the selected clusters. If we do so, the process of selection will involve sampling work to be done at two stage, one for selecting the clusters where sampling units are the clusters and the second for selecting the units (elements) within the selected clusters where sampling units are the elements.

ABSTRACT

India is the second largest producer of vegetables in the world after China with an annual production of 162.19 million tonnes from an area of 9.2 m ha. The requirement of vegetables in the country has increased a lot to meet the nutritional requirement of an estimated 1200 million population expected by 2020-21. Although the productivity levels of vegetables have increased manifold, it would not be sufficient to feed the ever increasing population. Production per unit area of land, time and inputs can be increased by improving efficiency of the rate and extent to which solar energy is harvested for conversion to economic produce. In multiple cropping systems, the possibility of more efficient use of resources like sunlight, nutrient and water per unit area is higher leading to higher production stability. It can be very much successful in high imput requiring crops like vegetables. Intensive cropping involving multi-tiered vegetable crops like pointed gourd + elephant foot yam + cowpea grown on the single piece of land at the same time can generate profits up to Rs 1,30,000 per acre. India ranks first in the youth population. If the energy of these dynamic youth is properly utilized in the field of better agricultural practices, especially vegetable cultivation, it will help improve the national productivity of vegetable crops and generate employment as a whole. Thus, intercropping involving multi-storied/ multi-tier cropping system can serve as well-established practice for sustained supply of vegetables throughout the year through which rural youth can generate high income for sustainable livelihoods and increased per capita income.

Abstract

A study was conducted to evaluate the nature and magnitude of genetic divergence and also to identify divergent parents in one hundred eight sorghum germplasm collected from different agro eco-geographical areas in India. Mahalanobis D² statistics revealed considerable genetic diversity among the 108 genotypes of sorghum. The genotypes grouped into 11 clusters.

Multivariate analysis is essentially the statistical process of simultaneously analyzing multiple independent variables with multiple dependent (outcome or criterion) variables using matrix algebra. During the last two or three decades, multivariate statistical analysis has become increasingly popular. The theory has made great progress, and with the rapid advances in computer technology, routine applications of multivariate statistical methods are implemented in several statistical software packages. The traditional approach to the teaching of multivariate statistical analysis, as exemplified by Anderson (1958), relies heavily on advanced matrix mathematics. On the other hand, Hury and Riedwyl (1988) suggest that it is possible to understand most of the basic ideas underlying multivariate statistical analysis without a mastery of such mathematics, provided that these are conveyed with the help of real data sets. In this chapter we propose a non-mathematical data-driven approach for teaching multivariate statistical methods to students. Despite this, we are mindful of the need for students to know some basic linear algebra and univariate statistical concepts. Such basic knowledge provides students with the foundation necessary for the application of the appropriate multivariate statistical procedures and for the interpretation of results.

Introduction

Time series data is data that has been collected in a specific pattern with time stamps. (Nayak and Ghosh, 2013). This type of time-oriented data is collected at regular intervals, such as hourly, daily, monthly, or weekly. Time series data can be used to make accurate predictions in a variety of fields and domains, such as foreign currency rates, stock market trends, energy consumption estimates, and climate change. Machine learning and data mining techniques can be used to uncover hidden patterns in historical data and forecast future trends (Aftab et al. 2018a, 2018b, Mishra et al. 2017).

Weather forecasting based on historical data is a complex but very beneficial task that comes with several issues that must be resolved to achieve optimal results (Wu and Chau, 2013). Weather data includes attributes or features such as temperature, pressure, humidity, and wind speed. Machine learning techniques are used to forecast future weather conditions by identifying hidden patterns and relationships in historical weather data (Aftab et al. 2018b). Prediction of precipitation is an important part of the weather forecasting process. The use of machine learning and artificial intelligence techniques is regarded as a critical component in farmer services and policy formulation. Farmers require weather forecasting to plan their activities following the predicted weather. Accurate and timely rainfall prediction in farming, in particular, can be extremely beneficial in planning and security measures for operations, agricultural tasks, water reservoir systems, and construction and transportation activities (Chau and Wu, 2010). Forecasting rainfall will become increasingly important in the future.

It is an acute specific infection of the salivary glands especially of the parotids sometimes submandibular and sublingual glands also; occasionally the latter glands are affected first.

The most affected are the children or young adults of both sexes often living in a school or a barrack and the disease spreads rapidly from one person to another in close contact by droplets. This virus reaches the salivary glands by bloody or lymphatic because mumps is a systemic disease and may cause in addition, inflammation of the meninges, brain, pancreas and the gonads. The disease is more prevalent in winter and spring months. The incubation period is about 3 weeks.

The affected parotid glands and the surrounding tissues become hyperaemic and oedematous, the acinar cells disintegrate with round cell infiltration but later on, heal completely without any scarring.

Testes when involved have much oedema, perivascular infiltration with lymphocytes, localized haemorrhage, destruction of the germinal epithelia and blocking of the tubules with cellular debris. Only certain localized areas are affected, other parts remain unaffected.

Mungbean (Vigna radiata L Wilczek) is considered to be originated from Vigna sublobata. It is also known as green gram, an important short duration grain legume with wide adaptability, low input requirements, and the ability to improve soil fertility by fixing atmospheric nitrogen. Mungbean is well suited to a large number of cropping system and constitutes an important source of cereal based diets. Mungbean is used for different purpose. The major portion is utilized in making dal, curries, soup, sweets and snacks. The food values of mungbean lie in its high and easily digestible protein. Mungbean is grown throughout Asia, Australia, West Indies, South and North America, Tropical and subtropical Africa. However, India alone accounts for 65% of the world acreage and 54% of the world production. It is widely cultivated in India in different seasons; however, the maximum area is under kharif cultivation. Extremely plastic morphology, phenology and short maturity duration (60 days) makes the crop ideal as a catch crop, inter crop or relay cropping. It is grown as sole relay crop in rice fallows during rabi season in A.P., Tamilnadu, Karnataka and Orissa and sole catch crop during spring/summer season in Uttar Pradesh, Bihar, West Bengal, Jharkhand, Punjab, Haryana & Rajasthan (Table 1).

Munk (1950) built upon Sverdrup’s theory, using both the vertical and lateral eddy viscosity. Munk combined the basic features contributed by Ekman, Sverdrup and Stommel to provide the first comprehensive solution of the wind driven circulation, using the real wind field Munk included the effect of eddy viscosity in both the horizontal and the vertical dimensions (both A_h and A_z).

Muscle structure
• All muscles are composed of muscle fibres which are the structural unit of muscles
• In turn, every single muscle fibre is made up of numerous smaller components called myofibrils
• Functional unit of myofibril is sarcomere
Connective tissues associated with muscle
• Whole muscle is surrounded by a sheath of connective tissue called epimysium
• Whole muscle is divided into muscle bundles by perimysium formed out of connective tissue septa originating from the interior surface of the epimysium separating the muscle fibres
• The perimysium contains the blood vessels and nerves

The muscular system allows movement and necessary functions such as circulation, digestion, and breathing. It is made up of muscles and connective tissue, such as tendons. The muscular system also helps provide support and shape. In the body, muscles have primary functions to provide motion, maintain posture, and generate heat.

Historical Aspect
    Curare was the first known muscle relaxant which was used by South American Indians to immobilize wild animals. Hence the term curariform has been applied to the action of this group of drugs. 
    In year 1958, a plant alkaloid alcuronium was introduced but there were difficulties in reversing the action of this drug.
    Succinylcholine was first used in 1906. However, its paralyzing property was discovered in year 1949.
    Pancuronium was introduced in 1964.
 

Cutaneous muscle

The cutaneous muscle of abdomen and pelvis is the continuation of cutaneous muscle of thorax. This has been described under the muscles of thorax.

Abdominal fascia

Beneath the skin in the abdominal region, there are two layers of fascia, the superficial and deep, similar to those of the fascia of thoracic region.

The superficial fascia is next to skin. It is fused dorsally with the thoracolumbar fascia. Cranially it is continuous with the superficial fascia of shoulder and arm and caudally with that of the gluteal region. In the inguinal region it forms part of fascia of penis in male and of mammary glands in female animals. At the flank, it continues as fascia of thigh region.

The deep fascia forms the abdominal tunic. It is especially well developed as a thick sheet of tunic in the ventral and the adjacent lateral parts of the abdominal floor. It extends cranially over the ribs and external intercostal and the caudal digitations of the serratus thoracis muscles. Caudally it is attached to the external angle of the ilium and thoracolumbar fascia. The suspensory ligament of prepuce in males and that of the mammary glands (udder) in females is an extension of this tunic. It strongly supports the abdominal muscles to bear the weight of abdominal viscera.

Site of incision: Cut the skin over the back and loin region by making cranio caudal straight incision along the proximal part of 1st rib and the lateral margin of transverse process of lumbar vertebrae up to wing of sacrum and reflect the skin to mid dorsal aspect of back and loin.

Site of incision

Make a mid-lateral and mid-medial longitudinal incision on forelimb from elbow joint to 3rd digit. Joins the above incisions transversally on cranial aspect of elbow joint and 3rd phalanx, respectively. Remove the skin on dorsolateral aspect of limb

Site of incision: Make a mid-lateral and mid-medial longitudinal incision on hind-limb from stifle joint to 3rd digit. Joins, the above incisions transversally on cranial aspect at stifle joint and 3rd phalanx, respectively. Remove the skin on dorsolateral aspect of limb.

Site of Incision

Make an incision around the eyeball at the margin of orbit.Then remove the supraorbital process by cutting dorsal and ventral end to access inside the orbit. Remove all the attachments of eyeball from osseous orbit through carefull dissection. Then remove all the extraoccular and periorbital fat to expose the muscles of eyeball.

Site of incision- Make a mid-lateral and mid-medial longitudinal incision on forelimb from elbow joint to 3rd digit. Join the above incisions transversally on caudal aspect at elbow joint above and 3rd phalanx below, respectively. Remove the skin on palmero-medial aspect of limb.

Site of incision: Make a mid-lateral and mid-medial longitudinal incision on hindlimb from stiffle joint to 3rd digit. Joins the above incisions transversally on caudal aspect at stiffle joint above and 3rd phalanx below, respectively. Remove the skin on plantero-medial aspect of limb.

The muscles of fore limb (Fig. 5-3 A and B) are distributed in different regions of fore limb as muscles of shoulder girdle, shoulder, arm and those of forearm and manus. By means of the muscles of shoulder girdle the forelimb is attached with the trunk of the body by synsarcosis and bears the weight of the body. The rest of the groups of muscles act on different joints of the fore limb as extensors or flexors of these joints. The group of muscles acting on these joints originates on the bone of the proximal segment and insert on the bone of distal segment of the concerned joint.

Muscles of shoulder girdle

The muscles of shoulder girdle have been described with the muscles of thorax.

Muscles of shoulder

The muscles of shoulder arise on the scapula, act on shoulder joint and end on the humerus. These muscles are in two groups as the lateral group of muscles and the medial group of muscles.

The muscles of the head (Fig. 2-3 A) are described in four groups viz (1) superficial muscles including the cutaneous muscle. The other muscles in this group of muscles are the muscles of lips, cheeks, nostrils, eyelids and external ear. (2) the orbital muscles (3) the mandibular muscles and (4) the hyoid muscles.

The cutaneous muscle on the head of the ox is well developed and extensive. In most of the regions of the head it is intimately adhered to the skin. The superficial fascia forms a continuous layer beneath the cutaneous muscle in this region except the natural orifices like nostrils, mouth, eyes and the external ear. The deep fascia forms a continuous layer particularly on the temporal, buccal, and the pharyngeal parts of the head. A part of the cutaneous muscle on the frontal region is called the frontalis muscle. The muscle fibres of this muscle blend with the muscles of the eyelids and the contraction of fibres of this muscle causes elevation of the superior eyelid and forms wrinkles to the skin on forehead.

The fascia covering the hind limb is the superficial and deep fascia. The superficial fascia is very scanty lies beneath the skin. It has the same general disposition as in other regions of the body. The deep fascia is in the form of a thick strong sheet covering the superficial layer of muscles over the medial aspect of the hind limb. From this sheet extend several septa that pass between different muscles or muscle groups in the thigh region. The deep fascia of the leg region continues cranially with the fascia of the thoracolumbar region. Caudally it is attached to the aponeurotic tendon of insertion of sartorius and gracilis muscles. The part of the deep fascia that continues on the lateral aspect of the thigh is the fascia lata which is continuous with the gluteal fascia dorsally.

The muscles of the hind limb (Fig. 6-3 A and B) are arranged into different regional, positional as well as functional groups. The muscles of hip and thigh region are arranged in the lateral, medial and cranial groups. The muscles of leg and foot are arranged in dorsolateral and plantar groups. The dorsolateral muscles are generally the extensors of tarsus and digits while the plantar muscles are the flexors of tarsus and digits.

Site of incision: Make an incision from distal end of cranial margin of stifle joint to coxal tuber and extend it dorsally up to mid dorsal line of the body. Make another incision at mid caudal of thigh from stifle region to tuber ischii and extend it to mid dorsal up to the base of the tail and then connects the distal ends of both previous incisions and reflect the skin up to mid dorsal line of the body.

Site of incision: Abduct the hind limb well apart and remove the skin covering the medial aspect of thigh. To obtain this make an incision longitudinally along the entire length of pelvic symphysis. From both ends of this incision make vertical incision up to medial aspect of stifle joint and joins distal end of these vertical incision together and remove the skin to expose the muscles of medial aspect of thigh region. To expose deeper muscles incise the vertebral attachment of biceps femoris, reflect it caudally by incising muscle close to stifle than reflect middle gluteus muscle dorsocranially and observe the deeper layer of muscles.

Site of incision-Make a rostro-caudal incision along the ventral border of horizontal ramus of mandible then extend cranio-dorsally along caudal margin of nostrils up to middorsal line of face. From the caudal end of 1st incision make another incision dorsally up to base of the ear then passes cranially along the ventral aspect of eye up to mid dorsal line of face. Reflect the skin carefully from ventral to dorsal, carefully remove the muscle zygomaticus, malaris and nasolabialis as these are firmly attached to skin.

Site of incision

Remove the skin of intermandibular space.Then remove one of the rami of mandible by disarticulating temporo-mandibular joint at caudal end and cut the ramus just behind the mental foramen.

Cutaneous muscle

In the cervical region of ox, the cutaneus muscle, cutaneus colli is poorly developed. It is in the form of a narrow strip on the ventral part of the neck. It arises from the ventral median connective tissue strip of the superficial fascia and extends cranially covering the cervical part of the deep fascia. The fibres of this muscle fan out dorsolaterally and form the aponeurosis with the cervical fascia and join the cutaneus facei.

Fascia

The major groups of muscles of the cervical region are covered by the thick superficial layer of fascia. The fascia is abundant and well developed in the cervical region of the ox. The fascia of the neck is in two layers, the superficial and deep. The superficial fascia is attached to the skin, whereas the deep fascia is again in two parts, the superficial and deep parts. The superficial part covers the superficial structures in the neck viz. the trapezius and brachiocephalicus muscles in the dorsolateral aspect and the jugular canal with jugular vein, and the rhomboideus muscles on the ventrolateral aspect of the neck. The superficial parts of the deep layer of cervical fascia of two sides meet at the median line both, dorsally and ventrally. The deep part of the deep fascia is attached to the vertebrae and the longus colli muscle on either side at the median line and covers the trachea, oesophagus, carotid artery etc before joining ventrally.

Site of incision: Make a midventral incision on the neck from larynx up to thoracic inlet then make ventrodorsal incision from both ends of this incision up to mid dorsal aspect of neck region.

Site of incision

Remove the skin flap of pectoral region to expose the pectoral muscles.

Site of incision: Make a transverse incision below the anus between lateral margins of tuber ischia. Then from each end of this incision make an oblique incision caudo-cranially so that these join midventrally each other between the thighs. Remove the triangular flap of skin to expose the region.

Site of incision: Make a straight dorso-ventral incision through skin from the summit of dorsal vertebral spine to the cranioventral aspect of shoulder joint. Extend it up to the cranial margin of shoulder joint, then caudo-ventrally up to caudal most part of elbow joint. Make another ventrodorsal straight incision from caudal most point of elbow joint to the summit of vertebral spine then reflect the skin flap from ventral to dorsal up to mid dorsal line of the body. Remove the facia to expose the various muscles of this region.

Site of incision: Detach the limb from the trunk by cutting various muscles viz; pectoral muscles, serratus ventralis, latissimus dorsi, brachiocephalicus, omotransversarius, trapezius, rhomboideus and long head of triceps.

Site of incision: Remove the abdominal viscera to expose the sublumber region.

Site of incision: Make two incisions at mid-lateral aspect at base of the tail join them dorso-ventrally and reflect the skin caudally up the 4-5 inches.

Site of incision: Make a dorsoventral incision along caudal border of last rib up to mid ventral region of abdomen and another incision along the coxal tuber up to mid ventral region of abdomen. Joins the above two incision mid ventrally and reflect the skin up to mid dorsal aspect of lumber region.

Site of incision: Make a midventral incision on the sternum from cranial to caudal than from both ends of this incision make cranial incisions along the caudal border of shoulder and arm region to mid dorsal aspect of back and along last rib to mid dorsal aspect of back, respectively. Reflect the skin flap from ventral aspect to mid dorsal region of back.

Cutaneous muscle

The cutaneous muscle of the thoracic region is a part of the cutaneous muscle of the trunk. It arises from the interbrachial fascia on the medial surface of the arm and elbow and the lateral aspect of the arm and shoulder. It extends as a broad sheet between the shoulder and arm cranially and the stifle and thigh caudally and then upward to the middle of the dorsum and the tuber coxae.

Fascia of thorax

The fascia of the thoracic region is the caudal continuation of cervical fascia. It is in two layers, the superficial and deep. The superficial fascia is continuous with that of the skin and the cutaneous muscle. The deep fascia forms a well developed sheath for aponeurosis with the longissimus muscle. It is attached medially to the supraspinous ligament and the summits of the spinous processes of the thoracic vertebrae while laterally it is attached to the lateral surface of the ribs. From here it continues ventrolaterally and passes medial to the scapula under the rhomboideus thoracis muscle.

A branch of anatomy that deals with muscles and their accessory structures is called as myology and the body system concerned with myology is called muscular system. The muscular system is composed of specialized cells called muscle fibres because of their shape in the form of fibre or spindle. The plasma of these cells contains myofibrils which are contractile elements that cause contraction of muscles in a definite manner under the influence of a stimulus which is a unique property of muscles. These cells together constitute the distinctive muscular tissue.

Classification of muscles

Based on their morphological and functional characteristics, there are three types of muscles in the body.

Smooth muscle: The muscular tissue in this type of muscles is constituted by masses of spindle shaped cells and they occur mainly in the walls of hollow visceral organs including glands, the vessels, eye ball and hair follicles. These are nonstriated muscles. Contraction in these muscles is weak but sustained and mostly consistent and rhythmic. The action of this muscle is involuntary i.e. not under the will.

Cardiac muscle: The muscle fibres of this muscle are arranged in irregular masses and they form cross striations. The ends of some fibres come in contact with the sides of others forming a kind of syncytium. This is a striated muscle and its action is involuntary. This muscle occurs only in the wall of the heart and the immediate proximal ends of the major blood vessels like aorta, venacava and the pulmonary veins.

Key Points

• The main objective of managing open fractures is to neutralize further damage to the surrounding tissue while executing prompt, stable osteosynthesis and immobilization.

• Discomfort management should be considered because musculoskeletal diseases can cause severe discomfort.

Degenerative Myopathies

It is a degenerative disease of the musculature. Endocrine imbalances (hyperadrenocorticism, hypothyroidism, and hyperthyroidism), hypokalemia in cats, ischemic myonecrosis in recumbent cattle, post-exercise rhabdomyolysis, chemical agents, poisonous plants, and drug-induced degenerative conditions are some of the possible associations with it.

Somites are derived from paraxial mesoderm and they are located lateral to the developing notochord and neural tube. During the third week of gestation in domestic animals, the outlines of somites first become visible and in the fifth week of gestation the somite formation is completed. Somites differentiate into sclerotome, myotome and dermatome. They form skeletal, muscular and dermal elements i.e, vertebral column, muscles and dermis respectively.

The connective tissue, cartilage and bone all differentiate from mesenchyme which arises chiefly from mesodermal somites and lateral layer of somatic mesoderm while connective tissue of alimentary canal and lower respiratory tract is derived from splanchnic mesoderm. Mesenchyme is a spongy meshwork composed of branching cells spaces between which are filled by ground substance. The supporting tissues, connective tissue, cartilage and bone are derivatives of mesenchyme in which ground substance is gelly like, stiff and firm respectively.

Mushrooms are most ancient, best known and greatly admired fleshy macrofungi. Mushrooms are delicacies, much sought after and greatly valued foods. Wild mushrooms are mostly found in forests, tree trunks, pastures, lawns and road side during winter season and appear at surprising speed. Mushroom hunting or gathering is a popular hobby in Western countries. The wild mushrooms may be edible or unpalatable or poisonous. Poisoning by wild mushrooms is common and may be fatal or produce merely mild gastrointestinal disturbance or slight allergic reaction. The term toad stool is popularly reserved for inedible or poisonous mushrooms, but this classification has no scientific basis. This alarming term keeps many people away from these foods. It is important that every mushroom intended for eating be accurately identified. Mushrooms vary in colour, size, shape, growth habit, yielding ability and other characteristics.

STRUCTURE: Mushrooms are the fleshy fungi which constitute a major group of lower plant kingdom. Mushroom is the conspicuous umbrella-shaped fruiting body (sporophore) of certain fungi of the order Agaricales in the class Basidiomycetes but also of some other groups. Popularly, the term mushroom is used to identify the edible sporophores. The edible reproductive part consists of a stem and a cap. Gills found on the underside of the cap, are arranged like spokes in a wheel. The gills bear multiple spores which start new fungi. The sporophore emerges from an extensive underground network of fine thread like white growth known as mycelium. The mushroom, fruit body produced basidiospores at the tip of club like structures, called basidia, which are arranged along the gills of the mushroom. Beneath the mushroom, in the soil, is the mold colony itself, consisting of a mat of intercoiled hyphae, sometimes several feet in diameter. The mushrooms first appear as white tiny ball consisting of short stem (stipe) and a cap (pileus) which begins to open up like an umbrella. The delicate membrane or Veil (velum) enveloping the cap tears off, if allowed to develop fully, and lamellae (gills) radiating from the stalk into the cap become visible. These gills become darkened as the basidiospores (seeds) develop into millions and fall in the ground for starting their life cycle once again for second generation of mushrooms.