eChapters

ABSTRACT

The process of developing new crop varieties involves many steps and takes several years. In recent times, biotechnological advances have helped in reducing these considerably. Apart from the techniques of haploid culture and genetic engineering, one of the tools which is making it easier for scientists to select plant traits and develop new varieties is the use of molecular markers. Molecular markers can be used for (a) characterization of germplasm (b) varietal identification and clonal fidelity testing (c) assessment of genetic diversity (d) validation of genetic relationships and (e) marker- assisted selection. These have widespread applications in management of genetic resources as well as in breeding programmes. Marker-assisted selection has practically revolutionized breeding programmes. Traditionally, plant breeders have selected plants based on their visible or measurable traits called the phenotype. But this process can be difficult especially in the case of multigenic or quantitative traits. Molecular markers can be used to trace linkages with traits of importance. In the area of horticulture, this is a very useful tool in tree breeding, where the breeder has to wait for several years for phenotypic expression of the desired trait. A number of DNA marker technologies are now available and rapid advances are being made in the development of more sensitive marker technologies. The techniques involved, the various applications of DNA marker technologies in horticultural crops and future perspectives in the national context have been discussed.

The residues from production and processing of raw agricultural products such as fruits, vegetables, meat, poultry, dairy products and crops are called agricultural
wastes. The by-products of agricultural activities are also referred to as agricultural waste.

Important Points to be Remember

• Total number of essential nutrient elements required for plant growth -17
• Man made cereal – Triticale
• Father of India’s green revolution – Dr M.S. Swaminathan
• Na, Ve, Ni, Si, Co, Se and I - Beneficial nutrients
• Is added to prevent nitrogen losses from FYM decomposition-SSP

The green pepper or chilli is reported to be the native of tropical America (Thompson and Kelly, 1957). This was widely cultivated in Central and South America in early times and was unknown in Europe prior to the discovery of America. The chilli belongs to the family Solanaceae and genus Capsicum. Only four species are under cultivation, out of which the cultivation of the two, Capsicum pendulum and Capsicum pubescens, are restricted to South and Central America.

2.1 Introduction

Water is the most precious gift of the nature and it is also the most crucial element for sustainability of the life in the earth. India is the largest user of freshwater in the world. India’s total water consumption alone is the higher than any other continents. The Agricultural sector is the largest consumer of water followed by the domestic sector and the industrial sector. The role of irrigation water as one of the most essential inputs for crop cultivation can’t be denied. As a traditional productive input, it ensures production by acting as an agent of insurance against inadequate and inconsistent monsoon. Ultimately the outcome provides agricultural production stability. Thus, irrigation is of prime importance in cultivation of vegetable crops as it ensures favourable water balance within the root zone in addition to natural precipitation. It fulfils the crop- water demand and improves the crop production and effectiveness of other agricultural inputs. It is also an important limiting factor of crop yield, because of its association with several factors of plant environment, which directly influence the crop growth and development (Yaghi et al., 2013). The various irrigation methods under different system of irrigation differ with regard to extent of control, timeliness and adequacy of supply of irrigation water for crop cultivation. Consequently, the economic benefits and the costs due to these irrigation methods vary among different irrigation systems. The dominant method of irrigation practiced in large parts of the country is surface irrigation (basin, border and furrow) where the entire soil surface is almost f looded without considering the actual consumptive requirements of the crops. Frequent over or under irrigation create the problems of water stress or water logging leading to reduced irrigation efficiency (less than 30%).

In 1882, Robert Koch first isolated the bacterium, stained with alkaline methylene blue and vesuvin as counter stain and showed its etiologic relationship with tuberculosis. Ehrlich first developed acid-fast stain in 1882 and used for better staining of the bacteria which was adopted by Robert Koch. The acid-fast staining technique was modified later by Franz Ziehl and Friedrich Neelsen, accordingly, it is known as Ziehl-Neelsen (Z-N) staining. In 1890, Koch prepared a glycerine broth extract of the tubercle bacillus, known as ‘tuberculin’. He tried to prepare it as a curative agent; but it was used later in diagnosis. In 1895, Johne and Frothingham described the first case of paratuberculosis in cattle which became known as ‘Johne’s disease’. The causative bacterium was later isolated by Twort and Ingram in 1910.

In India, bovine tuberculosis is reported from several states since 1959 (Dhanda and Lall, 1959). The bovine paratuberculosis in India was first reported by Nanda and Singh (1940) from Haryana.

Greenhouses have become an essential part of modern agriculture as they offer controlled environments for plant growth, research, and horticultural practices. This chapter discusses the various applications of greenhouses, which include extending growing seasons, improving crop yields, supporting botanical research, and producing ornamental plants. Additionally, it emphasizes the importance of repair and maintenance in greenhouse operations. Understanding the repair and maintenance protocols is crucial to ensure the sustained functionality of heating systems, water management, and structural components.

1. INTRODUCTION

During fermentation, the fruit wines have to be monitored for different parameters to know the progress of fermentation and to control the quality of the finished products. It is essential to maintain the quality of the products, for which it is necessary to keep a complete record of wine fermentation. One such form is the fermentation control record or fermentation logbook, which is kept to monitor and record fermentation progress. It includes the name of fruit, variety, original characteristics of juice/pulp, amelioration, tank number, inoculum type and quantities, sampling time, date of start of fermentation and completion. It should also have daily record of temperature of fermentation, total soluble solid or ^oBrix reading, titrable acidity and volatile acidity, racking time/date and other comments on the fermentation. Fermentation control records should be completed and all new wines be entered in the bulk wine book inventory, the annual supplemental records can be kept in a file entitled, for example ‘Vintage season 2018’.

Imagine the lifeblood of a nation’s energy grid thinning to a trickle. This is the stark reality when prolonged droughts grip regions heavily reliant on the oncemighty force of hydroelectric power. While the allure of clean, renewable energy from flowing water has positioned hydropower as a cornerstone of electricity generation globally, its dependence on a consistent and abundant water supply renders it exceptionally vulnerable to the increasingly frequent and intense droughts sweeping the planet. This chapter embarks on a critical exploration of the intricate and often strained relationship between these two forces: the essential need for water in sustaining hydroelectric power and the devastating impact of its absence during drought conditions Moving beyond a simple definition, we will delve into the multifaceted ways in which droughts cripple hydroelectric facilities, from dwindling reservoir levels that starve turbines to the cascading effects on energy security, economic stability, and ecological balance. We will traverse the globe, examining compelling case studies from drought-stricken regions, revealing the tangible consequences for communities and industries. Furthermore, this chapter will unpack the complex interplay of factors that exacerbate

3.1 INTRODUCTION

A nursery is a place, where seedlings, saplings, trees, shrubs and other plant materials are grown under intensive care and maintained until they are ready for final transplanting in the main field. The importance of the best quality planting material as an initial investment is a well-realized factor for persons engaged in horticulture field. So, nurseries have great demand for the production of plants, bulbs, rhizomes, suckers and grafts. But in general, good quality and assured planting material at reasonable price is not available in most of the cases. Nursery is the basic need of horticulture. Plant propagation techniques and practices is the core of horticulture nurseries. The planting materials for horticultural plantations are raised from seeds and vegetative parts. The difficulty of procuring seeds and their rising cost makes it necessary to find means to increase seedling survival and growth.

10.1 INTRODUCTION

‘Tissue culture’ is commonly used as a blanket term to describe all types of plant cultures, viz., callus, cell, protoplast, anther, meristem, embryo and organ cultures (George, 1993). It relies on the phenomenon of cell totipotency. The different techniques of culturing plant tissues may offer certain advantages over traditional methods of propagation.

Growing plants in vitro in a controlled environment, with in-depth knowledge of the culture conditions and the nature of the plant material, ensures effective clonal propagation of genetically superior genotypes of economically important plants. Micropropagation has become an important part of the commercial propagation of many plants (George and Sherrington, 1983; and Zimmerman et al., 1986) because of its advantages as a multiplication system (Pierik, 1997 and Razdan, 2003). Several techniques for in vitro plant propagation have been devised, including the induction of axillary and adventitious shoots, the culture of isolated meristems and plant regeneration by organogenesis and/or somatic embryogenesis (Williams and Maheswaran, 1986; and Gautheret, 1985).

Tissue Culture is becoming as an alternative means to vegetative propagation of plants. In vitro growing plants are usually free from bacterial and fungal diseases. Virus eradication and maintenance of plants in virus free stage can also be rapidly achieved in cultures (Sharma, 2016).

Introduction

Nucleotide and protein sequences construct the core of Bioiformatics. These biological sequences form complex and very large datasets. Various Statistical techniques have been used for analysis and extraction of precise information form small as well as large biological datasets. Evolution and natural selection makes biological sequences redundant and statistically biased. Once the mechanisms involved in such biases have been characterized, their analysis and systematic investigation become valuable tools for use in predicting the properties of other biological sequences. This biased nature results in relative codon usage value among the synonymous codons or Relative Synonymous Codon Usage (RSCU) value. Codon usage variation within a single genome can be an important source of information about gene expression levels and events of horizontal gene transfer (Medigue et al., 1991). The analysis of codon usage patterns can be traced back to when first molecular sequence databases were being colleted (Grantham et al., 1981). In order to evaluate the codon and amino acid usage variation, multivariate analysis options like Correspondence Analysis, Principal Component Analysis, Cluster Analysis, Factor Analysis, Multidimensional Scaling and Eigenanalysis Ordination are available (Morrison et al., 1994). It is well established that codon usage by its nature is multivariate. These methods provide a low dimensional point representation of genes, where the proximity of gene specific points indicates (CoA) a similar codon usage of associated genes. Correspondence Analysis is an exploratory graphical approach that requires contingency tables and generates results in the form of tables, scattered plots and graphs. CoA can identify the major sources of variation in the dataset. The output from a CoA can be used to evaluate other aspects of genes, such as base composition, expressivity, aromaticity and location on the genome (Thiouse et al. 1997).

Orchard management within the framework of natural farming in India prioritizes sustainability, ecological harmony, and the use of native resources to sustain healthy and productive fruit trees. Unlike conventional horticultural methods, it avoids synthetic fertilizers, pesticides, and herbicides. Instead, it adopts an integrated approach focused on soil vitality, biodiversity, and resilience to climatic conditions.
The core of natural orchard practices lies in preparing the soil with organic inputs such as Jeevamrit, compost, cow dung, and mulch derived from crop residues or dry foliage. These enrich the microbial life in the soil and enhance its aeration, water-holding capacity, and fertility. Intercropping with legumes, vegetables, and medicinal herbs is encouraged to optimize land use and foster biodiversity, which helps in minimizing pest and disease outbreaks.
Mulching is essential around fruit trees to conserve soil moisture, suppress weed growth, and create a stable microclimate. The principle of “Waaphasa” or proper soil aeration is particularly emphasized, aiming to provide adequate oxygen to the roots without overwatering. Organic mulches like straw, dried grass, or sugarcane residue help maintain this balance effectively.
Pest and disease control is managed through natural bio-solutions like Agniastra, Neemastra, and Brahmastra—homemade preparations using cow urine, neem, and medicinal herbs. These are applied routinely to deter pests.
Eco-friendly methods such as installing bird perches, insect traps, and growing companion plants further support pest regulation.

Climate change affects livestock through heat stress, water scarcity, unstable feed supplies, altered disease patterns and extreme weather. This chapter examines how artificial intelligence supports climate-smart and sustainable animal husbandry. Machine learning, predictive analytics and IoT-enabled monitoring can optimize feeding, detect disease, assess welfare and improve the use of water, energy and other resources. Intelligent technologies can also assist in reducing greenhouse-gas emissions and developing adaptive production strategies. The chapter evaluates the contribution of AI to productivity, climate resilience and environmental responsibility while addressing problems such as insufficient data, limited infrastructure, implementation costs and ethical concerns. Future integration with sustainable agricultural frameworks is also discussed.

The whole plants or their parts have been used as source of medicine and health tonics both in medical and veterinary practice since time immemorial. Many of
the active ingredients in chemically manufactured drugs have been originally derived from plant components (e.g. pyrethroids). Despite availability of
the modern allopathic medicines, herbal medicines are still used on a very large scale. In the back drop of current global trade of herbal medicine and
intellectual property right (IPR) scenario, and to overcome side effects of ‘single golden molecular bullet’ drug technologies of twentieth century, there
is an upsurge of interest in traditional practices.

Introduction

Plastics has the versatile properties like light weight, durability, less expensiveness, and it is readily available for the consumers usage. Due to these diverse properties, the usage of the plastics is increasing day to day and their production has been increased drastically in the past 50 years (Cressey, 2016). At present, global plastics production has reached to 159 million tonnes. This diversified consumption has led to the production of enormous amount of plastic waste (Hahladakis et al., 2018) and their properties allows for long range transport in the ocean (Baalkhuyur et al., 2018). According to the Earth Action reports, nearly 68,64,299 tonnes of plastic waste will end up in nature due to imbalance between the plastics consumption and production. India is among 12 countries responsible for 52 % of the world mismanaged plastic waste (Plastic Overshoot Day: Report 2023).

Marine litter occurrence and impacts are common in worldwide marine systems (Bhattacharya and Khare, 2019) and they are degraded into micro sized particles called microplastics through a various physical, chemical and biological processes such as U-V light, wind and ocean currents (Solomon and Palanisami, 2016). Microplastics are < 5mm in size and are further divided into large and small microplastics of size 1–5 mm and 20 µm–1 mm, respectively (Loder and Gerdtz, 2015). Microplastics are classified into 2 categories: i) primary microplastics, intentionally produced as small sizes, used in cosmetics, microfibers from clothing, fishing nets, and their shape, size, colour, and chemical composition may vary depending upon the products. ii) Secondary microplastics are the tiny particles including fibres, fragments formed by the degradation or breakdown of larger plastics particles due to the UV rays from sunlight oxidation process. These particles are generated from the plastic films, accidentally or intentionally discarded fishing ropes and nets, plastic bottles and plastic bags etc. (Andrady, 2011).

Traditional livestock diagnosis is often reactive and may identify disease only after clinical symptoms become visible. This chapter introduces deep-learning methods for automated health monitoring, early disease detection and predictive analytics. Models analyse images, videos, body temperature, heart rate, activity patterns and historical health information. Convolutional neural networks extract visual features and classify diseases, while LSTM networks identify patterns in time-dependent physiological and behavioural data. Multimodal fusion improves the reliability of predictions by integrating different data sources. IoT-connected systems can generate real-time warnings and support timely intervention. The chapter compares deep learning with conventional approaches and evaluates accuracy, recall, false-negative rates and practical deployment.

11.1 Introduction

The application of greenhouse in agriculture is vast. Greenhouses are used for growing flowers, vegetables, fruits, transplants and varieties of certain crops in a controlled environment which is appropriate for the plants. The edible flowers are served in some good restaurants to grow them precise calculation is required. Sensor network can help to monitor and automate the activities in greenhouse. The objective of this chapter is to discuss the development of sensor nodes to monitor the growth of flower plants through XBee and internet of things. The system comprises of three sections- sensor nodes, local server and main server. Sensor nodes communicate to local server with XBee and then local server communicates the same data to main server through IoT.Fig.11.1 shows the generalized block diagram, sensor node is to be implemented on each flower pot and communicating to local and main server.

The increasing concerns about dwindling biological resources globally led to the Convention on Biological Diversity in 1992. This convention for the first time, recognized sovereign rights of States over their biological resources and emphasized that access to genetic resources should be only for environmentally sound purposes and should be subject to national legislations. The access has to be on mutually agreed terms which inter-alia would include recognition of associated Traditional Knowledge of indigenous communities and equitable benefit sharing arrangements.

Taking cognizance of the provisions of the CBD, and to deal with extensive pressure on our biological resources, India has enacted an umbrella legislation called Biological Diversity Act-2002 (No. 18 of 2003), and also notified the Biological Diversity Rules-2004 for guidance and compliance by various stakeholders, including the Union and State Governments, Non-state sectors, and individuals. The Act aims at conservation of biological resources as well as facilitating access to them in a sustainable manner and through a just process. The salient features of this act are as follows (NBA, 2004).

INTRODUCTION

The debate on climate change is going on. From the report of the Inter Governmental Panel on climate change (IPCC), a premier organization set up in 1998 by the World Meteorological Organisation and United Nations Environment Programme, it is known that the average surface temperature has increased over the 20th century by about 0. 6ºC. The number may seem insignificant but it is an unprecedented rise. In the past 150 years, the 11 hottest years were witnessed. It says that 1990s was the warmest decade and 1998 the warmest year since 1861. Worse, the Artic is warming twice as rapidly as the rest of the world and there is a distinct possibility of the Greenland Ice Sheet collapsing altogether and pushing up sea levels by several metres. It is predicted that 2035 is the year when Himalayan glaciers may totally disappear causing catastrophic disruptions. Unfortunately, India is among the countries that will suffer the most serious consequences as a result of global warming. Following is the probable effect of global warming.