eChapters

Introduction

With growing concern about climate change, environmental sustainability, soil health and food safety, conservation agriculture, organic agriculture and protected cultivation are emerging production systems being popular in India. The changing farm practices in these production systems invite many biotic stresses and demand novel approaches for their sustainable management for profitable farming. The dynamics of biotic stresses vary from system to system; therefore they require system specific approaches for sustainable management. The different agronomic/ plant protection measures practiced in above mentioned emerging systems are listed in Table 1. Protected cultivation is meant for the high value crops are grown, the management of biotic stresses is the foremost important to minimize losses. Organic farming demands more preventive measures for sustainable control of biotic stresses rather than curative measures because of complete restriction on use of harmful chemical pesticides. Conservation agriculture demands more of ecofriendly measures for biotic stress management with minimal use of pesticides. In this chapter, we discussed novel approaches for biotic stress management in emerging production systems.

The possibility of making genetic changes in the laboratory in plants, animals and microbes used in food started almost 10,000 years ago. Along with natural changes taken place during the evolution totally different species have come into existence. This has helped in increased food production with better taste and nutrition. The losses caused by biotic and abiotic stresses from fungi and bacterial pathogens have reduced.

Omics technologies based on genes and proteins involved in the nematode activities and the plant resistance responses are key factors to novel nematode management. Techniques such as next-generation sequencing of genomes and transcriptomes, RNAi, PIs, MAS, chemo-disruptive peptides and genetic transformation systems with reproducible results must be available within phytonematodes management strategies to enhance crop yields (Abd-Elgawad, 2022). Linking long-read sequencing to the use of high density genetic mapping can also support the detection and characterization of nematode-virulent genes. Progress in computational biology, bioinformatics, and analyzing the omics large-scale data can efficiently boost these techniques to offer accurate recognition of components and pathways engaged in nematode parasitism and plant response. Updated genome-editing devices will serve classical plant breeding and precisely translate how gene actions are linked to phenotypic performances. Yet, these methods must be cautiously used to avoid unwanted effects such as nematode virulence and pleiotropic impacts on qualitative and quantitative crop yields. Definite issues such as those related to the transfer of multiple disease resistance traits, gene original construct, and specificity of the virulent nematodes to the R-gene may arise in particular cases.

Horticulture has emerged as one of the potential agricultural enterprises in accelerating the growth of economy in India. It offers not only a wide range of options to the farmers for crop diversification, but also provides ample scope for sustaining large number of agro-industries which generate huge employment opportunities. Horticulture has been evolving in form of horticulture industry in our country owing to its significant role in nutritional security, poverty alleviation and employment generation.

Horticulture has emerged as one of the potential agricultural enterprises in accelerating the growth of economy in India. It offers not only a wide range of options to the farmers for crop diversification, but also provides ample scope for sustaining large number of agro-industries which generate huge employment opportunities. Horticulture has been evolving in form of horticulture industry in our country owing to its significant role in nutritional security, poverty alleviation and employment generation. The production of fruits, vegetables and flowers has acquired much importance in recent times due to their increasing demand. India has a wide variety of climate and soil on which different horticultural crops such as fruits, vegetables, ornamentals, medicinal and aromatic plants, plantation crops and spices are cultivated successfully.

ABSTRACT

The black pepper, ‘the king of spices’ cultivation fetches foreign exchange worth millions of rupees annually, has been constrained by the quick wilt disease caused by the fungal pathogen Phytophthora capsici. Peptides with systematic variations in cationicity, hydrophobicity and amphipathicity were designed based on Chou-Fasmann parameters and sequential alterations of amino acids were tested for antifungal activity against Phytophthora. The antifungal properties of these biodegradable and ecofriendly peptide peptides were evaluated following in vitro microplate titre assay using Phytophthora zoospores. Two peptides LD1 and LD4 showed very low MIC value as compared to that of well-known antifungal peptides and standard chemical fungicides. Fluorescent staining technique using fluorescent brightener-28 and propidium iodide dyes has revealed that the site of action of the designed peptides is the cell membrane. These peptides showed very low haemolytic potential inspite of their potent antifungal activity. The in vivo evaluation of the peptides on fungi infected detached pepper leaves has demonstrated their high antifungal potential.

Drying

Drying is one of the age-old techniques for preservation and value addition of food products. Generally, it means removal of moisture from the food products by evaporation. It is aimed at lowering the water content of food stuff, thereby arresting the growth of microbes, action of enzymes and other autolytic chemical reactions (Naidu et al., 2016). This preservation technique is predominately used for food materials which are regarded as “highly perishable”. The benefits of drying includes extended shelf-life, lower storage space, reduced packaging requirements, lower handling and transportation costs, off-seasonal availability and importantly diversified product for the ultimate consumers (Bonaui et al., 1996).

Fish drying

Open sun drying as a method of fish preservation is recorded in history by the primitive societies. However, open sun drying of fish has few draw backs which includes poor product quality, higher microbial contamination and complete dependence of weather conditions for drying (Bala & Mondol, 2001). In the later stages, drying developed into completely a controlled process in which drying air temperature, relative humidity and air velocity were precisely maintained at optimum level. The fish drying process is slightly different from other food materials mainly due to its gel like behaviour until considerable reduction of moisture. Moreover, significant amount of shrinkage takes place in fish during drying in addition to the other irreversible changes.

The Government of Tamilnadu has taken a number of progressive measures on water resources and irrigation management, particularly through the Bank -assisted Tamil Nadu Water Resources Consolidation Project (WRCP) which was closed in September 2004 with a satisfactory rating. Now the Tamilnadu Government has decided to implement a project for the modernization of the Traditional Irrigation systems in Tamil Nadu with loan assistance from World Bank. The World Bank has also come forward to assist the Tamilnadu Government’s proposal. Under the World Bank funded project of “Irrigated Agriculture Modernisation and Water Resources Management” (WRCP - II) about 17 River sub basins have been selected and proposed to be taken up under the control of Water Resources organisation Wing of Public Works Department of Tamilnadu. The Varahanadhi sub basin is one among the seventeen. Accordingly this ‘IAMWARM’ Project report for the modernization of the irrigation systems of Varahanadhi Sub Basin has been prepared. The very objective of this project is to attain maximum productivity from farm lands, for which serious attention is required to modernise the existing irrigation structures, anicuts, canal systems and tanks in order to make them functionally more effective, conserve and utilise the available water from catchment area for optimum use.

As the heart diseases in companion animals is becoming common as in humans, the research in canine cardiac diseases is one of the most extensive studies in the present scenario. As per the available literature, the overall occurrence of various cardiac abnormalities ranges from 0.37 percent to 1.67 percent in various parts of the country. Whereas, in western parts of the globe, nearly 8 million dogs suffer from heart disease accounting 10% of all dogs in the United States. Heart diseases are common among older dogs; up to 75% of senior dogs have some type of heart condition, and unfortunately, most go undiagnosed due to varied reasons. The recognition of canine cardiac diseases has been delayed, and ignored on account of lack of awareness and knowledge by the owner and inadequate diagnostic facility in our country. Physical examination, radiography, electrocardiography and echocardiography are most useful diagnostic procedures employed in diagnosing the cardiac diseases in dogs. However, majority of the acquired cardiac diseases are being managed medically with one or a combination of drugs. Improved diet management and health care of companion animals have led to increased life expectancy, as a result, cardiac diseases have become pivotal causes of death for both dogs and cats. For such reason, a major objective has become to increase the quality of life by reducing the expansion of cardiac diseases via early detection of diseases and provision of effective treatment.

A new buzz-word is rapidly spreading through the manufacturing and analytical environment. Discussions on the application of Non Invasive Research namely the Near Infrared (NIR) Spectroscopy are often found on the agenda of production, project, and technical and even board meetings. Engineers and chemists are confronted more frequently with the challenge to decide whether a NIR instrument will be the solution to their production quality control and other analytical problems. Are there really amazing machines that can determine protein, fat, moisture, fibre, starch, nicotine, alcohol, sugar, amino acids and whatever analyses you require on any product from foodstuff to fertilizer and animal feeds in less than a minute and so simple to operate that even the unskilled labourer can handle it? In this article we will endeavor to answer the questions that often crop up in the evaluation of NIR as an analytical technique towards Natural Products.

7.1. Developing Sector in Scientific and Technological Approach of Waste Management

From the very past, many traditional practices have been put into use for waste management like landfilling, composting, and recycling, which are practiced even today. However, the boundless upsurging waste compels the modern world to modify the traditional methods or to introduce innovative techniques with the grace of technology

With the increasing health consciousness of consumers, there has been an increasing concern over the use of thermal processing methods like blanching, pasteurization, sterilization etc. for enhancing the shelf life of foods as these methods fail to preserve the fresh like nutritional, therapeutical and sensory qualities of the food. With the increased consumer demand for fresh and fresh-like foods, interest in minimal processing of foods is increasing. The trend is also shifting from the traditional heat processing to those technologies that employ no or minimum heat application, thereby retaining the quality to a maximum level. One of the ways to accomplish this is via use of non-thermal processes. Over the past few years a number of non-thermal processes or cold preservation methods have been invented and developed. These methods are being evaluated in pasteurization and sterilization of various foods or in the extension of shelf life of various packaged foods. Some of such novel technologies and the products prepared, have been discussed here under.

1. Extrusion

Extrusion is a size enlargement process wherein small granular food or powdered particles are re-formed into larger pieces with different shapes, textures, colours and flavours from the basic ingredients. In extrusion food is compressed and forced to pass through a fine opening (die). As soon as it comes out of the opening, the outside pressure is very less as compared to the pressure inside the screw of extruder and the food expands instantaneously. If the food is heated the process is called as extrusion cooking. It is a process which combines several unit operations including cooking, mixing, kneading, shearing, compressing, shaping and forming. Extrusion adds a variety to the products by changing shape, size, texture and even some of the minor ingredients of the food and at a reduced cost than most of the other processing methods. Extrusion cooking (not extrusion) is a high temperature short time (HTST) process that reduces microbial contamination and inactivates enzymes.

Abstract

Sedentary lifestyle coupled with excess calorie intake is partially responsible for increased incidence of lifestyle related diseases like coronary heart disease, obesity, diabetes, cancer and hypertension. Non-nutritive sweeteners can offer consumers a way to enjoy the sweet taste with little or no energy. Further, functional beverages are the drinks which are altered in such a way to provide specific health benefits and disease preventing properties beyond general nutrition. In the present paper, authors have summarized various research works on development of different low calorie functional fruit and vegetable products by replacing sugar with non-nutritive sweeteners like saccharin, cyclamate, sorbitol and stevioside at different levels of sweetness (equivalent used to sucrose). The cyclamate sweetened spiced plum squash at 50 per cent sweetness proportion gave higher taste scores in comparison to saccharin sweetened beverage. In case of sorbitol sweetened beverages, with the increase in the proportion of sorbitol sweetness, the organoleptic score for colour, body and flavour increased compared to saccharin and cyclamate sweetened products. Whereas, the stevia sweetened beverages indicated highest sensory acceptability at 75% stevioside sweetness level. Further, sorbitol sweetened health products were found to have similar calculated energy value as that of sucrose sweetened products (100%), but sorbitol is absorbed slowly and does not raise the insulin levels. The development efforts had successfully reduced calorie value of plum squash up to 42% with cyclamate at 50% sweetness level. The calorie value of the sorbitol and stevioside sweetened products has been reduced by 70% and 60% per serving at 100% and 75% sweetness level, respectively. The developed products were found to have strong antimicrobial activity against E. coli as well as high antioxidant potential. Overall, it was concluded that, the developed products had better taste, palatability and storage stability beside reduced energy value, hence their availability in the market will definitely benefit the at risk group i.e. diabetics, obese and health conscious people.

A. Concentrated Organic Manures

Introduction

Human resources are the most important assets of an organization. The success of an organization depends on the talent and caliber of the employees working therein. It is true in the case of service-oriented institutions like libraries and Information centres. Libraries of the university are a treasure trove of information and knowledge. The library is one of the important components of a university. Library and Information Science (LIS) professionals are the assets of libraries and information centres. Libraries are undergoing transformational changes across the world as a result of disruptive technologies. Libraries are becoming informal, relaxed and collaborative spaces.

INTRODUCTION

Northeast India comprises the contiguous seven sister states.viz., Arunachal Pradesh, Assam, Manipur, Meghalaya, Mizoram, Nagaland and Tripura and the Himalayan state of Sikkim. The Northeastern states of India are blessed with diversified fish fauna which assumes special interest due to the fact that the region is grained by two important drainages viz., Brahamaputra-Barak and Chindwin Irrawaddy. (Gurumayum and Choudhury, 2009). Riverine fisheries resources of Northeastern States comprise of 19,150 km of streams and rivers. Except the Brahmaputra and Barak Valleys in Assam and Imphal Valley in Manipur, the Northeast mainly comprises of Hills and Mountains which form 65% of its territory (Suguman, 1998).

Introduction

Indian fisheries and aquaculture is an important sector of food production providing nutritional security, besides livelihood support and gainful employment to more than 14 million people, and contributing to agricultural exports. With diverse resources ranging from deep seas to lakes in the mountains and more than 10% of the global biodiversity in terms of fish and shellfish species, the country has shown continuous and sustained increments in fish production since independence. The sector constitutes about 6.3 percent of the global fish production and contributes to 0.91 percent of the GDP and 5.23 percent of the agricultural GDP. The total fish production of 13.34 million metric tonnes presently has nearly 74 percent contribution from the inland sector and rest by the marine sector. Paradigm shifts in terms of increasing contributions from inland sector and further from aquaculture have been significant over the years. With high growth rates, the different facets, viz., marine fisheries, coastal aquaculture, inland fisheries, freshwater aquaculture, and coldwater fisheries are contributing to the food basket, health, economy, exports, employment and tourism of the country. More than 50 different types of fish and shellfish products are being exported to 75 countries around the world. Fish and fishery products have presently emerged as the largest group in agricultural exports from India, with 13.77 lakh tonnes in terms of quantity and ` 45,106.89 crore in value. This accounts for around 10% of the total exports and nearly 20% of the agricultural exports, and contribute to about 0.91% of the GDP and 5.23% to the Agricultural GDP of the country.

The Pradhan Mantri Matsya Sampada Yojana (PMMSY) is an initiative launched by the Government of India to establish a comprehensive framework and reduce infrastructural gaps in the fisheries sector. The scheme was announced by the Finance Minister, Smt. Nirmala Sitharaman during her speech in the Parliament of India while presenting the Union budget for 2019–20 on 5 July 2019. The government intends to place India in the first place in Fish production and processing by implementing Blue Revolution. This scheme is in line with government’s aim to double the farmers’ income by 2022–23.The policy envisages integrating all the fishermen with agricultural farmers and providing all the facilities available through various farmer welfare schemes to the fishermen. A new dedicated Department of Fisheries was constituted in a newly carved out Ministry of Fisheries, Animal Husbandry and Dairying to implement this and other policy initiatives of the government.

The study of soil organic matter is becoming increasingly important by the day as world agriculture attempts to ensure sustainability of soils, while at the same time striving to enhance food production to feed an ever-burgeoning population. The use of green manures and pasture leys, the return of residues and additions of organic amendments to the soil are often resorted to in an attempt to increase soil organic matter which immensely benefit the physical, chemical and biological make-up of the soil.

Introduction PCR is an important molecular tool used widely in different applied research fields such as Infectious disease diagnosis, clinical medicine development and gene cloning etc., (Fang et al., 2008). Among different amplification methods, PCR was the first to be developed and has been the method of choice for its simplicity, easier methodology, extensively validated standard operating procedure and availability of reagents and equipment’s. However, it also has a good number of limitations such as advanced equipment, high cost, sensitivity to certain classes of contaminants and inhibitors requirement of thermal cycling etc. (Fakruddin, 2011). These limitations of PCR allowed to develop the alternative methods such as loop mediated isothermal amplification (LAMP) (Notomi et al., 2000), nucleic acid sequence-based amplification (NASBA) (Compton, 1991), self?sustained sequence replication (3SR) (Guatelli et al., 1990), rolling circle amplification (RCA) (Lizardi et al., 1998) etc., most of these techniques are isothermal that don’t require thermal cycler. These methods have many advantages over PCR in terms of speed, cost, scale or portability.

Introduction

Nucleic acid based diagnosis is highly specific, sensitive and rapid method widely used in health management. The detection is mainly based on structure and sequence of bases of nucleic acid which are unique to an organism. In recent years, nucleic acid based diagnosis is becoming common and popular in fish and shell fish health management. Commonly employed nucleic acid based diagnosis are DNA hybridisation and Polymerase chain reaction (PCR).

Nucleic acids are made up of nitrogen bases. The primary sequence of bases in DNA or RNA have regions which are unique for a particular organism or gene. The sequence of DNA is composed of a series of four phosphorylated bases Adenine (A), thymidine (T), guanidine (G) and cytosine (C). Pairs of bases (A=T, or G=C) can form hydrogen bonds, which cause opposing strands of DNA to form a semi-stable bonding (hybridization or annealing) to one another. If the opposing strands of DNA match exactly, then the familiar double helix form of DNA results. In the following example, the opposing strands in (1) will form a double stranded, tightly bonded structure, and the DNA strands are described as complementary. In (2) the strands are non-complementary (opposing A=T and G=C base pairs do not line up) and the two strands will not bond.

DNA Hybridisation

The basic principle in DNA hybridization is that, if a strand of double helix of DNA is separated from its complement (by heat) it will bind (reanneal or hybridize) only to its complementary strand (or to a duplicate of its complementary strand). Two complementary RNA structure will hybridise in the same manner. Inaccurate and mismatched bindings will be unstable and fall apart.

The binding of one DNA strand to another is in some respect is analogous to binding of antigen and antibody. However, in the case of nucleic acid bindings, the precise molecular nature of binding (base pairing) is known and can be easily manipulated by choosing DNA strand of appropriate length and by incubating the strands in appropriate salt concentration, temperature. The stringency (faithfulness of the match) of hybridisation can be precisely defined and as a result coincidental cross reaction is virtually eliminated.

Nucleic acid extraction from clinical samples is the first step to be followed in molecular detection of cancer. Nucleic acids are the starting material for all downstream molecular diagnostic tools. Quality of test results largely depends upon the quality of starting material. Thus utmost importance should be given to the extraction protocols to isolate good quantity and good quality nucleic acids from patient samples.

Basic principle: The basic principle of nucleic acid extraction is to disrupt cell membrane and nuclear membrane to release and precipitate nucleic acid in the solution. Following precipitation,removal of other cellular contaminates such as protein, lipids, polysaccharides etc is performed to obtain a good quantity and quality of nucleic acid.

DNA Isolation Protocol
Introduction

Most of the all procedures involved in molecular biology experiments require the purified form of nucleic acid. It forms a fundamental requirements for genome characterization, gene mapping and for identification and isolation of gene for genetic engineering. The most important step involved in removal of proteins which is carried out in different ways. The following protocol is one of the most commonly used protocols in animal, plant and microbial samples.

The search for the chemical basis of genetic material began about one and half century ago. Soon after discovery of Mendelian principles of segregation and independent assortment in 1865, Friedrich Miescher (1844-1895) published a method for separating cell nuclei from the cytoplasm and from these cell nuclei, he extracted an acid material termed as “nuclein” which had unusually large amount of phosphorous. Miescher concluded that this substance was unique and was just not comparable with any other group known at that point of time. Later on nuclein, now named as “nucleic acid” was found to be associated with various proteins and the combined entities were termed as “nucleoproteins”.

Nucleic acids were first discovered in 1868 by Friedrich Meischer. Nucleic acids are high molecular weight polymers that are present in our cells. They store and transfer genetic material from generation to generation. Knowledge of how genes are expressed and how they can be manipulated is becoming increasingly important for understanding of nearly every aspect of biochemistry. These macromolecules p ass on information for cellular growth and reproduction. All the genetic information in the cells is called the genome. Every time a cell divides, the information is copied and passed on to the new cells.

Nucleic acids like proteins occur in all living cells. They device their name because of their primary occurrence in the nucleus and acidic nature. Deoxyribose nucleic acid (DNA), one of the two nucleic acids is a major component of chromosomes. Small amounts of DNA are also found associated with chloroplasts and mitochondria.

Nucleic acids were first discovered in 1868 by Friedrich Meischer. Nucleic acids are high molecular weight polymers that are present in our cells. They  store and transfer genetic material from generation to generation. Knowledge of how genes are expressed and how they can be manipulated is becoming increasingly important for understanding   of nearly every aspect of biochemistry. These macromolecules pass on information for cellular growth and reproduction.  All the genetic information in the cells is called the genome. Every time a cell divides, the information is copied and passed on to the new cells.
 
Nucleic acids fall into two main classes according to the type of sugar they contain: the Deoxyribonucleic acids (DNA) and Ribonucleic acids (RNA). Some sections  of  the  DNA  called  genes contain the information to make a protein. RNA translates the genetic information in the DNA to the ribosomes, where the synthesis of proteins takes place.

Introduction

The nucleic acids are the molecular depots that are responsible for continuity of life by transfer of hereditary information from one generation to next. The sequence of nitrogenous bases containing programmed instruction determines the structure of every cell constituent and eventually every protein. In 1869, Friedrich Miescher, Swiss chemist, isolated an unknown phosphate-rich substance from pus cells named as nuclein, which was quite different from other organic substances like carbohydrate, protein, and fat. Altmann in 1899 ascertained that the nuclein also had some acidic characteristics and changed its name to ‘nucleic acid’. Later, nucleic acid was also isolated from salmon sperm nuclei as DNA-protaminecomplex. In 1953, James D.Watson and Francis H.C. Crick constructed the model of three-dimensional double helix structure of the DNA molecule which could successfully explain DNA replication.

Nucleic acids are polymeric macromolecules, or large biological molecules, essential for all known forms of life. Nucleic acids, which include DNA (deoxyribonucleic acid) and RNA (ribonucleic acid), are made from monomers known as nucleotides. Each nucleotide has three components: a 5-carbon sugar, a phosphate group, and a nitrogenous base. If the sugar is deoxiribose, the polymer is DNA. If the sugar is ribose, the polymer is RNA. Together with proteins, nucleic acids are the most important biological macromolecules; each is found in abundance in all living things, where they function in encoding, transmitting and expressing genetic information—in other words, information is conveyed through the nucleic acid sequence, or the order of nucleotides within a DNA or RNA molecule. Strings of nucleotides strung together in a specific sequence are the mechanism for storing and transmitting hereditary, or genetic, information via protein synthesis.

The term nucleic acid is the overall name for DNA and RNA, members of a family of biopolymers, and is synonymous with polynucleotide. Nucleic acids were named for their initial discovery within the nucleus, and for the presence of phosphate groups (related to phosphoric acid). Although first discovered within the nucleus of eukaryotic cells, nucleic acids are now known to be found in all life forms as well as some nonliving entities, including within bacteria, archaea, mitochondria, chloroplasts, viruses and viroids. All living cells contain both DNA and RNA (except some cells such as mature red blood cells), while viruses contain either DNA or RNA, but usually not both. The basic component of biological nucleic acids is the nucleotide, each of which contains a pentose sugar (ribose or deoxyribose), a phosphate group, and a nucleobase. Nucleic acids are also generated within the laboratory, through the use of enzymes (DNA and RNA polymerases) and by solid-phase chemical synthesis. The chemical methods also enable the generation of altered nucleic acids that are not found in nature, for example peptide nucleic acids.

Nucleic acids are complex, chain-like polymers consisting of nucleotides as their monomers. Due to this characteristic, they are often referred to as polynucleotides. The largest molecules present in the biological system are nucleic acids. The two main types of nucleic acids are DNA (deoxyribonucleic acid) and RNA (ribonucleic acid). The bulk of DNA is confined to the nucleus and some cell organelles like mitochondria and chloroplast. RNA is found in both the nucleus and cytoplasm. In 1869, Johannes Friedrich Miescher isolated a substance from the nuclei of the pus cell (leukocytes). The extracted material was named nuclein, which contained an unusually large amount of phosphorus; in addition to proteins, and devoid of sulfur. Richard Altman later named the nuclein as nucleic acid. These organic molecules are found in living cells and play a crucial role in transmitting genetic information from one generation to another.

Nucleotides

Nucleotides serve as the fundamental constituents of nucleic acids. Nucleotides have flat planar and heterocyclic origin. Nucleotides are small molecules composed of three components:

1. Nitrogenous base,

2. Pentose sugar and

3. Phosphate group.

Procedure

Place a portion of your RNA sample (approximately 40 mg, hydrated) into a heavy walled Pyrex test tube. Add 1.0 ml of 1 N HCl and seal the tube.

Heat the tube in a boiling water bath for 1 hour.

Cool the tube, open it and place the contents into a centrifuge tube. Centrifuge the contents at 2,000 RPM in a clinical centrifuge to remove any insoluble residue. The supernatant contains your hydrolyzed RNA.

Prepare Whatman filter paper No. 1 for standard one-dimensional chromatography.

Using a micropipette, spot 20 µl of your hydrolyzate onto the paper, being careful to keep the spots as small as possible (repeated small drops are better than one large drop). Allow the spots to completely dry before proceeding.

Place the paper chromatogram into your chromatography tank and add the solvent (acetic acid:butanol:water). Allow the system to function for an appropriate time (approximately 36 hours for a 20 cm descending strip of Whatman #1). Remove the paper and dry it in a circulating air oven at 40° C for about 2 hours.

Locate the spots of nucleotides by their fluorescence under an ultra-violet light source. Expose the paper chromatogram to a UV light source and outline the spots using a light pencil. The order of migration from the point of origin is guanine (light blue fluorescence), adenine, cytilic acid and finally, uridylic acid.

After cafefully marking the spots, cut them out with cutters and place the paper cut outs into separately labeled 15 ml conical centrifuge tubes. Add 5.0 ml of 0.1 N HCl to each tube and allow the tubes to sit for several hours to elute the nucleotides from the paper.

Leaves are alternate in distichous arrangement; lamina long and linear and leaf sheath covering the internodes; ligule membranous and auricle claw shaped. The inflorescence is a spike and florets are borne on rachis node. Each spikelet has three florets. In two row barely, lateral spikelets become sterile.

Agathi is a fast growing tree upto 10m. tall. Branching is drooping. The stem bark is deeply furrowing forming cork like plates. Leaves are compound, pinnae, 20 to 30 cm. long glabresence to pubescent. Leaflet oblong rounded 18 to 24 pairs of leaflets that are green to dark green mostly, bitter free. Inflorescence is a raceme 4 to 7 cm long and bearing 2 to 5 flowers that are white, red, yellowish and pink. Flowers are 5 to 10 cm long, 3cm wide, curved calyx bell shaped 2 cm long, 2 lobed, fine teeth like serration, fleshy. 

Crop improvement is a continuous process. Crop improvement aims to genetically enhance, enrich and empower the crop plants with superior performance, enhanced yield level, higher quality of food, nutritional, fodder, energy produce and adaptability to climate change and crop growing environments. The intrinsic virtues of the improved cultivars could be sustained mainly through the production of the quality assured seeds endowed with genetic capabilities. The quality assured seed delivery system is through the seed chain from the basic seeds through Nucleus-Breeder-Foundation-Certified seed. Quality seed production system need to be based on genetic, breeding and seed technological protocols including field and laboratory testing to ensure very high genetic purity, true to type and superiority.

In mathematics, we use different type of digits called numbers. These numbers may be real, natural, rational, irrational, composite and complex, imaginary, perfect, positive and negative integers, whole number, prime and co prime numbers and even-odd numbers and make students confused. Therefore the concept of numbers should be very clear to the students so that they may answer the questions easily.

The number system mainly into classified into 8 types.

1.1 Introduction to Numerical Method

There are three types by which problem / system can be solved and are analytical methods, graphical methods and numerical methods.

Analytical methods: This approach or methods are used to find the exact solution of simple problems / systems rather than complex systems. It provides the behavior and characteristic features of a particular system, but can only be used for systems which can be approximated through use of linear models. Another limitation is in the case of practical problems, consisting of complex and nonlinear equations / processes, as analytical methods cannot handle properly.

Graphical methods: This approach or methods are used to find the approximate solution of problems / systems through drawing of graphs for each of the equation and then check for the intersection of graphs. Intersection of graphs represents solution to system of equations, and no interaction or parallel lines of graphs represents no solution. This is not useful because of non-precise nature due to graphs drawing through hands, which also makes the process tedious. Another limitation is its viability for problems involving three or lesser dimensions.

Numerical methods: This approach or methods are used to find the approximate solution of problems / systems in a simpler way involving a large quantity of long arithmetic calculations, as compared to analytical methods. Numerical methods are the techniques by which mathematical problems are formulated to find the solution through arithmetic operations.

4.1 Introduction

Numerical differentiation is the process of computing the function’s derivative values from the given set of numerical values when the actual form of the function is not known.

Similar to the numerical interpolation, a number of formulae for differentiation are derived in this chapter and are

1. Derivatives Based on Newton’s Forward Interpolation Formula: This is used to compute the derivative for some given x lying near the beginning of the data table.
2. Derivatives Based on Newton’s Backward Interpolation Formula: This is used to compute the derivative for a point near the end of the data table.
3. Derivatives Based on Stirling’s and Bessel’s Interpolation Formula: This is used to compute the derivative for some point lying in the middle of the data table.
4. Derivatives Based on Newton’s Divided Difference Formula: This is used to compute the derivative for some point when the data table is not equi- spaced.

Abstract

Groundwater flow modeling in hydrological sciences is one of the main tools used to assess the resource potential in terms of quantity and to predict the future impacts under different stresses/scenarios. This case study is prepared to present the  groundwater flow model for the Osian-Mandore pilot area with 12 villages of Mandore Block and 21 village of Osian Block in Jodhpur district of Rajasthan. The spatial model parameters and spatio-temporal fluxes were acquired and processed using remote sensing, GIS and Groundwater flow modeling interfaces. MODFLOW software was used to model the groundwater flow system in three interfaces PMWIN 5.3.0, Groundwater Modeling System and Visual MODFLOW. 

7.1 Introduction

A Scottish mathematician David Gibb was firstly introduced a term ‘Numerical Integration’ in 1915. Like numerical differentiation, numerical integration is also a very useful tool for scientists and engineers especially when they have an experimental data for different variables without knowing any mathematical relationship between them. Beside it, there are also many other situations where it plays an important role for integrating the functions like:

Problem 1: Prepare a lay-out plan of model kitchen garden for a family having five (5) members. Calculate the total amount of vegetable required per year and area of the same kitchen garden. Give the suitable crop rotation and show by diagrammatic representation.

Ans.: Total amount of vegetables required annually

Problem-Recommended dose of N:P:K for bearing mango tree 1.0 Kg N: 0.87 Kg P and 1.66 Kg K annually per plant. Available fertilizers are urea, DAP and MOP for supplying of NPK respectively.

So, for an orchard of 1 hectare area, the total number of plants is 100 if spacing is 10m x 10m

Problem- Calculate the amount of farm yard manure, urea, single super phosphate and murate of potash for one hectare tomato crop which require 100, 60, 60 kg ha N, P, K respectively. 1/3 quantity of nitrogen should be given through farm yard manure and rest through chemical fertilizers

Problem- Prepare a layout plan for establishing on ideal mango orchard near Varanasi city in 2ha area under high density planting. Calculate the number of plants needed for planting and amount of DAP and sulphate of potash heeded or planting. Also mention the name of cultivars for this region under HDP (200:100:200 NPK g/plant)

Problem-Prepare of a layout plan of orchard for 10 ha. area which is situated on the highway. The dimension of the orchard is 3:2. Calculate the areas under each crop which will you grow and also write their suitable variety along with their suitable diagram. Also fulfill the following information about orchard

1.     A fruit juice at 20°C with 10% total solids is concentrated in a single effect evaporator that is operated at a vacuum to allow the product moisture to evaporate at 80°C and steam with 82% quality is supplied at 169.06kPa. The desired concentration of the final product is 45% total solids and the concentrated product exits the evaporator at a rate of 300kg/hr. Calculate the steam requirement for the process when condensate is released at 90°C. The specific heat of liquid feed is 4.05 kJ/kg°C and of concentrated product is 3.175 kJ/kg°C.

(a) 3.35 kg/s

(b) 3.25 kg/s

(c) 4.25kg/s

(d) 2.35kg/s

2.     A double effect evaporator is being used to concentrate fruit juice at 35000kg/hr. The juice has 10% total solids at 80°C and requires to be concentrated to 50% total solids. The saturated steam at 1.608 atm is available. What is mass flow rate of water vapors?

(a) 7.505 kg/s

(b) 8.505 kg/s

(c) 5.707kg/s

(d) 6.808.kg/s

Aim

To study the numerical problems on grain moisture content and equilibrium moisture content.

Problem 1

Five hundred kilograms of paddy at 22% moisture content (wb) is dried to 14% moisture content (wb) for milling. Calculate the amount of moisture removed in drying.

8.1 Introduction

In the fields like Physics, Chemistry, Biology, Engineering and Economics; their laws and principles contains various parameters and their derivatives. The mathematical relationships of such parameters with their derivatives constitute the differential equations. In calculus, differential equations were firstly introduced by Newton and Leibnitz. Newton listed the three kinds of differential equations:

Man has tendency to classify everything in a hierarchical manner for example, ships may be classified on size, weight, what they carry or naval versus merchant or passenger. Any of these classification is as valid as any other.

But classification of biological species is unusual in that a predefined system exists i.e. the evolutionary relationships among organisms. But evolutionary relationships can only be estimated; and contrary to popular belief, even a good fossil record does not conclusively show the phylogeny or evolutionary tree of a group species.

Numerical taxonomy can be defined as “Numerical evaluation of the affinity or similarity between taxonomic units and ordering of these units into taxa on the basis of their affinities”. The term includes drawing of phylogenetic inferences from the data by statistical or other mathematical methods to the extent to which this should prove possible. The practice involves a number of fundamental assumptions and philosophical attitudes toward taxonomic work.