eChapters

Cryptosporidiosis is a protozoan disease.

Species

1. C. meleagridis: Infects small intestine.
2. C. baileyi: Infects digestive tract (bursa of Fabricius and cloaca), kidneys and respiratory tract.

Chemically, sugar candies are broadly divided into two groups: crystalline candies and amorphous or non crystallized candies. Crystalline candies are not as hard as crystals of the mineral variety, but derive their name and their texture from their microscopically organized sugar structure, formed through a process of crystallization, which makes them easy to bite or cut into. Fudge, creams, and fondant are examples of crystalline candies. Amorphous candies have a disorganized crystalline structure. They usually have higher sugar concentrations, and the texture may be chewy, hard, or brittle. Hard candies, such as lollipops, caramels, nut brittles and toffees are all examples of amorphous candies, even though some of them are as hard as rocks and resemble crystals in their overall appearance. Crystalline candies are chemically described as having two phases, because the tiny, solid sugar crystals are suspended in a thick liquid solution. These are also called grained candies, because they can have a grainy texture. Amorphous candies are have only one phase, which is either solid or liquid, and do not have a grainy texture, so they may be called ungrained.

Commercially, candies are often divided into three groups, according to the amount of sugar they contain:

3.1 Introduction

Crystallography is the science of crystals. The term crystal is derived from the Greek word “Crystallos” which means “ice”. The term was used for colorless quartz which was believed to be “fossilized” ice. Crystals are bodies bounded by flat surface, arranged on a definite plan which is an expression of the internal arrangement of the atoms. They are formed by consolidation of magma which is also considered as crystallization.

3.2 Terms in Use to Describe a "Crystal"

3.2.1 Faces

Faces are the plane surface in a crystal. The common faces are usually parallel to net planes containing the greatest number of lattice points or ions. Wide spacing between the net planes leads to development of faces in these planes. Faces are two types like and unlike faces.

The Mineral Kingdom presents a dazzling floral-display in the form of natural crystals. This is what makes mineralogy a very fascinating subject both for an amateur as well as an expert. Unless one visits the celebrated Lechuguilla cave in New Mexcio, USA it will be difficult to visualize the breathtaking beauty that crystals present. This, over 160 km cave is lined with gigantic as well as delicate gypsum crystals- as thin as human hair to several centimetres across and 6 m long . Crystals come in all shapes, sizes and colours. Their beauty and regular shape has not only attracted the scientists but made them objects of magical and mythical powers in many cultures of the world.

The study of crystals - including their growth, structure, classification by form and their physical properties forms the subject of crystallography. As of today one can’t think of this subject without x-ray crystallography, however, this being not in your syllabus only an introduction will be presented in the last chapter, while the entire section will be devoted to fundamental aspects of crystal morphology.

The section comprises four chapters. Chapter I describes and introduces the theme, presents a brief historical background of the development of the subject and gives some fundamental definitions. Chapter II presents the morphological elements including symmetry elements( plane, axes and centre of symmetry), parameters, indices, notation of crystal faces and other related aspects which are the basis for dividing crystals of all shapes and sorts into six or seven systems and several sub-types. This chapter will help you understand as to why mineral galena belongs to cubic system, while gypsum is monoclinic, or as to why an octahedron is represented by a symbol (111) but a cube with (100). Chapter III describes the seven systems and their sub-types and ends with an account of twinning- a curious feature quite often encountered among crystals. The section ends with a preliminary idea about stereographic projection and x-ray crystallography.

Cucumber (Cucumis sativus var. sativus L., 2x = 2n = 14, normally monoecious, cross-pollinated) is a member of the economically important family Cucurbitaceae which includes squash/pumpkin (Cucurbita ssp.), watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai], melon (Cucumis melo L.) and gourds. After tomato (Solanum lycopersicum L.) and watermelon, cucumber and melon are cultivated more widely than any other vegetable species globally accounting for about 2.5 million ha and producing 43 million tons giving a productivity of over 17 tons/ha under field and greenhouse culture. Production of cucumber is the second largest of all cucurbits, where China, Iran, Russia, Turkey and the United States represent about 75% of the world production.

During 2017-18, India produced 1.25 million tons of cucumber from 0.08 million ha (80, 000) area resulting into a productivity of close to 16 tons/ha (MAFW, 2018). Top ten cucumber producing states in India in the same period are Haryana (17000 ha), MP (9500 ha), Karnataka (8300 ha), Assam (6900 ha), Maharashtra (4500 ha), Andhra Pradesh (4400 ha), Bihar (3900 ha), Punjab (3600 ha), Kerala (3400 ha) and UP (3300 ha).

Cucumber, both slicing and pickling types, is one of the most popular vegetable crops of the family Cucurbitaceae. Like other cucurbitaceous crops, the cucumber also requires a warm season for commercial cultivation and has little or no tolerance to frost. Temperatures above 20°C are optimal for cucumber growth and development. Currently, its cultivars are generally classified as either pickling or slicing types, each with its own set of quality attributes. Pickling types typically have more prominent warts (tubercles) and lighter-coloured skin. They are harvested more frequently at the immature stage, when their length and diameter (LD) ratios are lower. The LD ratio for pickles typically falls within the range of 2.8 to 3.2. On the other hand, slicing cucumbers are harvested and commonly sold in lengths ranging from 12 to 25 cm, with diameters ranging from 1.5 to nearly 7.5 cm. Most slicing cucumbers have cylindrical-shaped fruit with blocky or slightly rounded ends that taper slightly from the stem to the blossom end. Generally, slicers have an LD ratio greater than 4.0, a smooth surface, and thicker skin compared to pickling types. This difference is accentuated because slicers are harvested at later stages of maturity than pickling cucumbers.

Cucumber, Cucumis sativus under the family Cucurbitaceae (Chromosome No: 2n = 2× =14) is grown throughout the world for consumption of fresh and tender fruits as slicing cucumber, and for pickles as pickling cucumber. Slicing cucumbers are usually larger and have thicker skins, while pickling cucumbers are usually smaller and have thinner skins. The flesh of cucumbers is rich vitamin C, and folic acid, fibre and a range of minerals including magnesium, molybdenum and potassium.
Origin, domestication and adaptation in India
The genus Cucumis, which includes cucumber (Cucumis sativus) and melon (Cucumis melo), has numerous wild African species, and it has therefore been assumed that
melon originated in Africa. For cucumber, this seemed less likely because wild cucumbers exist in India and a closely related species, Cucumis hystrix is available in the Eastern Himalayas. Using DNA sequences from plastid and nuclear markers for some 100 Cucumis accessions from Africa, Australia, and Asia, Sebastian et al (2010) concluded that melon and cucumber are of Asian origin and have numerous previously overlooked species-level relatives in Australia and around the Indian Ocean.

Vegetable crops belonging to the family Cucurbitaceae are known as cucurbits. These include 950 species in over 90 genera, mainly distributed in the tropics and subtropics (Schaefer and Renner, 2011). Enormous diversity of cucurbit genetic resources has evolved in past centuries to cater the nutritional requirements of people in different geographical and climatic regions. A large number of cucurbitaceous vegetables coming from different centres of origin are cultivated worldwide (Table 1). However, these crops are challenged by several biotic and abiotic stresses. Cultivated varieties are constrained by market demands, the necessity for climatic adaptations, domestication bottlenecks, and in most cases, limited capacity for interspecific hybridization, creating narrow genetic bases for crop improvement. Genetic resources are the building block for any crop improvement programme and includes landraces, traditional varieties, wild, weedy and other related species constituting primary, secondary and tertiary gene pools. They are valuable sources of important genetic traits needed for developing resilient and climate smart crop varieties and therefore, important for food security. The success and pace of the development depends on the efficient use of plant genetic resources. Wild relatives are incredible genetic resources carrying several desirable attributes like nutritional quality, resistance to biotic and abiotic stresses etc. Most of the crop wild relatives of cultivated crops across the world are not receiving adequate attention for their effective utilization in breeding programs. It is therefore, of utmost priority to protect them in the wild, through in-situ conservation, where they can continue to evolve and develop these useful adaptations. ICAR-National Bureau of Plant Genetic Resources (ICAR-NBPGR) is the nodal agency for the management of national wealth of vegetable genetic resources speciallyworking for collections, characterization, identification and documentation of new germplasm. Besides, it also bears the responsibility for conservation of the germplasm for future through sustainable use. The Vegetable germplasm is being conserved both under in-situ and ex-situ genebanks. This chapter highlights the status of Cucurbit genetic resources and procedures to access the germplasm by various stakeholders for research use.
 

Bitter Gourd

Genetic Resources

Bitter gourd or balsam pear (Momordica charantia L.) is one of the most popular cucurbitaceous vegetable, commonly cultivated in many countries. Probably, India is the centre of origin and secondary centre of diversity of this crop. Bitter gourd is an annual monoecious herb, belongs to the genus Momordica that comprises 60 species. Out of these, only M. charantia, M. cochinchinensis, M. dioica, M. balsamina (balsam apple), M. cymbalaria (syn. M. tuberosa) are under cultivation. Momordica charantia is grown all over the country in tropical and subtropical climates and rich genetic diversity occurs in northern plains including tarai region, the northern-western plains, the central region/plateau and the western and eastern peninsular regions. Kakrol (M. dioica) is cultivated in West Bengal, Assam, parts of Bihar, while sweet gourd (M. cochinchinensis) is popular particularly in Tripura, Assam, Andaman Nicobar, Tamil Nadu, Uttar Pradesh, Bihar and West Bengal. M. denundata (Thw) Clarke (syn. M. dioica var. denundatais) is found abundantly in Kerala, M. macrophylla in Assam and M. sabangulata Blume is commonly found in Karnataka, Maharashtra, Meghalaya and Sikkim. In India, NBPGR , New Delhi, and IIVR, Varanasi, are actively engaged in the collection of bitter gourd germplasms.The NBPGR, New Delhi, has evaluated 33 collections for 37 characteristics. Variability has been reported in vine length (105-335 cm), number of primary branches (3-7), number of fruits/plant (1-25), fruit girth (8-27 cm), fruit length (9.5-20 cm) and fruit yield/plant (0.5-2.8 kg). Accessions, UB-22, UB-66, IC-74158, C-1621 and K-3374, have been found better yielder. At IIVR, Varanasi, 121 genotypes have been evaluated for yield and yield-contributing traits. VRB-40 & IC-44415A have been identified as promising lines. Accessions showing better charecters for different traits are given below (Ghosh and Kalloo, 2000):

Improved Varieties of Cucurbits

A. CUCUMBER, MELONS, PUMPKIN AND SQUASHES

Cucumber (Khira)

Cucurbits account for the largest group of tropical vegetables, consisting of cucumber, muskmelon, bitter gourd, bottle gourd, ridge gourd, sponge gourd, snake gourd, pointed gourd, bitter gourd, ivy gourd, wax or ash gourd, chow chow or chayote, pumpkin, summer squash, winter squash, watermelon etc. They all belongs to the family Cucurbitaceae. They are herbaceous in nature and are characterized by tendril-bearing vines and adaptation to warm climate of the tropics and subtropics. Among the different genera of cucurbit vegetables, three genera viz., Cucumis (cucumbers, melons), Cucurbita (pumpkins, squash), and Citrullus (watermelons), are economically important in the world while the others hold regional importance. Most of the cucurbits are grown for its immature fruits and are consumed as salads or cooked vegetable. While, muskmelon and watermelon are used fresh as dessert fruits. Pumpkin and squashes are used in confectionaries besides as cooked vegetables. Mature ash gourd fruits are used in sweet candy preparation.

Cucurbits are low in calories, minerals, or vitamins. However, bitter gourd is rich source of vitamin C, the squash, pointed gourd, and muskmelon contain good amounts of carotene, kakrol is high in protein, and pointed gourd is rich in calcium. Bitterness in some species of cucurbits was attributed by the varying degree of bitter principles, cucurbitacin. Bitter gourd is known for its hypoglycemic activity due to phytoceutical, cheratin in the fruit, which lowers blood sugar level.

1) Downy Mildew
Diagnostic Symptoms
• Yellow, angular spots restricted by veins resembling mosaic mottling appear on upper surface of leaves .
• Corresponding lower surface of these spots shows a purplish downy xgrowth in moist weather.
• Spots turn necrotic with age.
• Diseased leaves become yellow and fall down .
• Diseased plants get stunted and die .
• Fruits produced may not mature and have a poor taste (Fig.-1).
Etiology
Scientific Classification
Kingdom : Fungi
Division : Eumycota

Cucumber

Cucumber (Cucumis sativus) is a warm season vegetable used as salad and for pickling purpose has little or no frost tolerance.  Growth and development favoured  by temperature above 200C.  Cucumber cultivars generally classified as pickling or slicing types. In India often cucumbers (Cucumis sativus) are confused with melons (Cucumis melo ) as morphologically some variants of these species overlap, but they are not cross compatible. Kakri of North India are different from kakri of South India and Maharashtra. In Maharastra and South India cucumbers are referred as kakri but, in North India Kakri means Long melon i.e. Cucumis melo Var. utilissima.

Fruit flies: Bactrocera cucurbitae:Tephritidae

This pest is more serious in bitter gourd, snake gourd and to a certain extent on ridge gourd. Other cucurbits are attacked to a lesser level. Damage by this pest causes losing the fruit quality, rotting and fruit drop. The adults lay many eggs inside the fruit sing their big ovipositor that hatch out into worm like apodous maggots. Feeding by the maggots on the pulp of fruits result in rotting, yellowing and fruit drop. The dropped fruits allow pupation by the maggots in the soil from where they emerge out as adults and start egg laying on fresh frits.

Crops belonging to family Cucurbitaceae are generally known as ‘Cucurbits’. It consists of a wide range of vegetables either used for salad purpose (cucumber) or for cooking (all gourds), pickling (West Indian gherkin) or as dessert fruit (muskmelon, water melon) or candied or preserved (ash gourd). As a group, cucurbits occupy the largest area in India and in other tropical countries. Its use is not primarily because of calories, minerals or vitamins since they are generally low to moderate in nutrients with a few exceptions like bitter gourd (rich in vitamin C- 96mg/100g), pumpkin (vitamin A 1600 IU/100g), Kakrol (high protein 3.1g/100g,), parwal and chow chow (calcium- 531 mg and 140 mg / 100 g respectively). From the nutritional point of view, cucurbit seeds are valued for their high oil and protein contents. Seed proteins of cucurbits are comparable to that of legumes and are richer in methionine.

The family Cucurbitaceae comprises about 117 genera and 825 species in warmer parts. Chakravarthy (1982) estimated 36 genera and 100 species in India.

Crops belonging to family Cucurbitaceae are generally known as ‘Cucurbits’. It consists of a wide range of vegetables either used for salad purpose (cucumber) or for cooking (all gourds), pickling (West Indian gherkin) or as dessert fruit (muskmelon, water melon) or candied or preserved (ash gourd).  As a group, cucurbits occupy the largest area in India and in other tropical countries. Its use is not primarily because of calories, minerals or vitamins since they are generally low to moderate in nutrients with a few exceptions like bitter gourd (rich in vitamin C- 96mg/100g), pumpkin (vitamin A 1600 IU/100g ), Kakrol (high protein  3.1g/100g,), parwal and chow chow (calcium- 531 mg and  140 mg / 100 g respectively). From the nutritional point of view, cucurbit seeds are valued for their high oil and protein contents.  Seed proteins of cucurbits are comparable to that of legumes and are richer in methionine.

1.1 Introduction

‘Cucurbits’ is a term coined by Liberty Hyde Bailey for cultivated species of the family Cucurbitacae. During this current century the term has been used not only for cultivated forms, but also for any species of the family Cucurbitacae. Cucurbits are frost sensitive, predominantly tendril-bearing vines which are found in subtropical regions around the globe. A few numbers of species those are native to or cultivated in temperate climate are prolific seed producing annuals or perennials that live for one season until killed by frost (Robinson and Decker-Walters 2004). Other vernaculars applied to the family Cucurbitacae and several of its members are ‘gourd’, ‘melon’, ‘cucumber’, ‘squash’ and ‘pumpkin’.

Cucurbits form an important and a big group of vegetable crops cultivated extensively in this country. This group consists of a wide range of vegetables, either used as salads (cucumber) or for cooking (all the gourds) or for pickling (cucumber) or as dessert fruits (muskmelon and watermelon) or candied or preserved (ash gourd). They are of tremendous economic importance as food plants.

2.1. Ash Gourd (Benincasa hispida)

Ash gourd (Benincasa hispida L.) belongs to the family Cucurbitaceae. The winter melon, also called white gourd, ash gourd, or “fuzzy melon”, is a vine grown for its very large fruit, eaten as a vegetable when mature. It is the only member of the genus Benincasa. This is a monotypic genus and the only cultivated species is Benincasa hispida (Thunb) Cogn, commonly known as ash gourd or petha. It has a Sanskrit equivalent ‘Kooshmanda’ indicating its antiquity in India.

Originally cultivated in Southeast Asia, the ash gourd is widely grown in China, India and other parts of East Asia. The immature melon has thick white flesh that is sweet when eaten. By maturity, the fruit loses its hairs and develops a waxy coating, giving rise to the name wax gourd, and providing a long shelf life. The ash gourd is known by many other names in India regional wise like komora (Assamese), pethakaddu (Hindi), boodagumbala (Kannada), Kumbalanga (Malayalam), Kohla (Marathi), neer poosanikai (Tamil) and Boodida gummadikaya (Telugu).

Uses

Ash gourd have enormous medicinal properties as it makes a valuable place as a popular natural cure of ailments in traditional medicinal practice. Being low in calories it is an ideal vegetable for diabetic patients and good for weight control. It also acts as a good detoxifying agent.

As per Ayurveda, ash gourd is used to treat epilepsy, asthma, lung diseases, urine retention, internal haemorrhage, and cough. It is said that the fruit juice is also effective in cases of mercury poisoning and snakebite. It could also treat mouth cancer, protecting teeth and gums when a mouth gargle of the juice is done regularly. It is also effective in bleeding of gums. Ash gourd is easily digestible and keeps the body cool. It is a natural blood purifier.

The Cucurbitaceae family consists of nearly 100 genera and over 750 species. Although most of them have Old World origin (Whitaker and Davis, 1962), many species originated in the New World and at least seven genera have origins in both hemispheres (Esquinas-Alcazar and Gulick, 1983). There is considerable genetic diversity within the family and the range of adaptation for cucurbit species includes tropical and subtropical regions, arid deserts and temperate locations. A few species are adaptable to production at elevations as high as 2000 m. The genetic diversity in cucurbits extends to both vegetative and reproductive characteristics. There is considerable range in the monoploid (x) chromosome number (Jeffrey, 1990) including 7 (Cucumis sativus), 11 (Citrullus spp., Momordica spp., Lagenaria spp., Sechium spp., and Trichosanthes spp.), 12 (Benincasa hispida, Coccinia spp., Cucumis spp. other than C. sativus, and Praecitrullus fistulosus), 13 (Luffa spp.), and 20 (Cucurbita spp.).
 
Archaeological evidence indicated that cucurbits were present in ancient and prehistoric cultures. Lagenaria was associated with man as early as 12,000 BC in Peru (Esquinas-Alcazar and Gulick, 1983). Archaeological expeditions in the Oaxaca region of Mexico have reported that Cucurbita pepo was associated with man as early as 8500 BC and cultivated by 4050 BC (Esquinas-Alcazar and Gulick, 1983). Written Chinese records describing the use of cultivated cucurbits have been found as early as 685 BC (Herklots, 1972).

Food Animals are the main protein source for human nutrition in the world.Sub-therapeutic doses of antibiotics are commonly used in food animal production, through their addition in feed and wateras antibiotic growth promoters (AGPs). Animal production is considered one of the main sources of antimicrobial resistances (AMR), thus the research for alternative strategies to the use of antibiotics in animal diet is essential to f ight the emergence of AMR. Currently, AMR causes the death of 700,000 people per year and it is forecasted that this will increase exponentially to 10 million by 2050, becoming the main cause of death worldwide.The reduction of antimicrobial resistance is a major challenge for the scientific community. In a few decades, infections by resistant bacteria are forecasted to be the main cause of death in the world. The withdrawal of antibiotics as growth promoters and their preventive use in animal production is essential to avoid these resistances, but this may impair productivity and health due to the increase in gut inflammation. The best alternative to Antibiotic growth promoters (AGPs) is a general improvement of conditions for food animals. In order to start a reformation of the Food Animal Industry as a whole, it’s essential to research to find out effective, resistance rare, low toxic and affordable alternatives to antibiotic growth promoters. Culinary herbs derived bioactives may be the promising products to address this challenge because it is traditionally proven to restore the gastrointestinal health due to beneficial effects on microbiota composition, mucosal barrier integrity, and immune system to control inflammation.