eChapters

This chapter focuses on split plot and strip plot designs, essential tools in experimental design known for effectively handling complex experiments. It covers the key principles and applications of these designs, emphasizing how they can be used to address specific research challenges. The chapter also provides practical guidance on conducting analyses of split and strip plot designs using R software, making it a valuable resource for researchers seeking to apply these methods in their work.

Split Plot Design

The split-plot design is a statistical approach widely used in experiments where specific conditions apply:

• Hierarchical arrangement of treatments: This design is ideal for experiments involving two levels of experimental units, typically “whole plots” and “subplots.” The main treatments are applied to the whole plots, while the sub-treatments are applied within these to the subplots.

• Adherence to experimental design principles: The split-plot design follows the principles of randomization, replication, and local control, but with a hierarchical twist. The main treatments are randomized across the whole plots, and the sub-treatments are randomized within these main plots. • Resource management in unequal experimental conditions: This design is particularly useful when some factors require larger experimental units or when changing levels of one factor is more resource-intensive than changing levels of another factor.

In this chapter, we will explore multiple comparison tests, with a particular emphasis on three significant tests: least significant difference (LSD), Bonferroni, and the techniques to visualize their outcomes. We aim to facilitate a comprehensive understanding of how to conduct and interpret the results of these comparison tests visualization using the R software.

Treatment Comparison

The “doebioresearch” package is commonly used for testing the significance of treatment groups in various experimental designs such as randomized block design (RBD), completely randomized design (CRD), and least significant difference (LSD). This package excels in conducting multiple comparison tests like LSD, Duncan’s multiple range test (DMRT), and Tukey’s honestly significant difference (HSD). It efficiently calculates all comparisons in the background, resulting in the generation of only group labels, as previously demonstrated in the output of the “doebioresearch” package. Additionally, the “agricolae” package is another useful tool that provides detailed values for multiple comparison tests. These three tests—LSD, DMRT, and HSD—are particularly popular in agricultural experiments and form the focus of our discussion.

I. Least significant difference (LSD) test

• To conduct the least significant difference test for comparing treatment means, the “agricolae” package is essential.

• To install the “agricolae” package, use the following code:

• install.packages (“agricolae”)

• After installation, activate the package with this command:

• library (“agricolae”)

• The data frame required for analysis in this context remains consistent with what is needed for randomized block design (RBD), completely randomized design (CRD), LSD, and variability analysis, among others. As an illustration, consider an example involving variability analysis.

Biomimetics is defined as the study of the formation, structure, or function of biologically produced substances and materials and biological mechanisms and processes especially to synthesize similar products by artificial mechanisms which mimic natural ones.

Biomimetic materials and molecules have been applied in many of the potential research areas in medical and dental sciences and highlight their foundation from plants, animals, or microbes that can be used to treat human disease.

A bioactive/biomimetic material and biocompatible material is an artificial or natural material used to change part of the living system or to function in internal contact with living tissue. Bioactive materials can interact with living tissues or systems.

There are several types of biomimetic materials like

Osteogenic Materials

Osteoconductive Materials

In the past, there was no targeted development of biomaterial based on scientific criteria. Instead devices consisting of material that had been designed, synthesized and fabricated for various industrial needs (the textile, aerospace, and defense industries) were tested in a trial and error fashion in the bodies of humans and animals. These unplanned and sporadic attempts had modest success. Most frequently, the results were unpredictable, mixed, and confounding both in success and in failure. In addition to traditional medical devices, diagnostic products, pharmaceutical preparations and healthcare disposables, now the list of biomaterial application includes smart delivery systems for drugs, tissue cultures, engineered tissues and hybrid organs. Undoubtedly biomaterial have had major impact on the practice of contemporary medicine and patient care in both saving and improving the quality of lives of the humans and animals4.

The craniofacial region presents a unique challenge for implantable biomaterials because the pull of the craniofacial muscles produces variable loading in different regions. In addition, craniofacial skin loses elasticity with the age and develops subcutaneous tissue sags.

Even though surgeons are familiar with the use autogenous bone grafts for craniofacial reconstruction, the usage of alloplastic bone substitutes has increased continuously, because autogenous bone is difficult to harvest, time consuming, causes significant donor morbidity, and bone resorption, but most important thing is that the biocompatibility of current alloplastic materials are improving.

Synthetic substitute creates a new avenue for clinicians to explore as adjunct in surgical procedures. These materials may not necessarily be used solely for reconstructive procedures, but when used in the right situation in combination with autologous, allograft or other synthetic, the result have the potential for more desirable results.

1. Jefferies SR. 2014. Bioactive and biomimetic restorative materials: a comprehensive review. Part I. J Esthet Restor Dent. 26(1):14-26.

2. Sharma M, Murray PE, Sharma D, Parmar K, Gupta S, Goyal P. 2013. Modern approaches to use bioactive materials and molecules in medical and dental treatments. Int J Curr.Microbiol. App. Sci., 2(11):429-39.

3. Laskin Daniel M: Oral and Maxillofacial Surgery vol 1

4. Kay C. Dee, David A. Puleo, Bizios R. 2002. An Introduction to Tissue Biomaterial Interactions.

5. Peltola MJ, Vallittu PK, Vuorinen V, Aho AA, Puntala A, Aitasalo KM. 2012. Novel composite implant in craniofacial bone reconstruction. Eur Arch Otorhinolaryngol. 269(2):623-8

6. Kao ST, Scott DD. 2007. A review of bone substitutes. Oral Maxillofac Surg Clin North Am. 19(4):513-21, vi.

7. Nasr HF, Aichelmann-Reidy ME, Yukna RA. 1999. Bone and bone substitutes. Periodontol 2000. 19:74-86.

• Avian Inflammation

Inflammation is a process which begins following a sublethal injury to tissue and ends with complete healing or death. It is characterized by five cardinal signs. Cardinal signs of inflammation are:

• Redness

• Heat

• Swelling

• Pain

• Loss of function

• Inflammation is beneficial in most of cases expect

• In prolonged or unending process

• In inflammation of immunological origin i.e. hypersensitivity and autoimmunity.

• It provides basic foundation of pathogenesis and pathology.

• It is now said that immunity is the resistance of body, while inflammation is the process by which the immune mechanism are implemented.

• The basis of inflammation remains same be it mammal or birds. For details, one can refer the chapter inflammation and heading in book “Illustrated Veterinary Pathology”. However, there are some differences in reaction in birds which are briefly listed below:

Pathology of nutritional deficiency diseases are described in chapter etiology of this book. However, specific deficiency of nutrition that leads to development of clinical signs and lesions in poultry are summarized as under (Fig.2.1 & Fig.2.2):

Vitamin Deficiency

1. Hypovitaminosis A

• Vitamin A is considered infection resisting vitamin because it facilitates the proper development of bursa, immunity and epithelium on mucosal and skin surfaces.

• Deficiency of vitamin A leads to swollen glands in oesophagus i.e. nutritional roup.

Ranikhet Disease

Ranikhet disease is a contagious viral disease of poultry characterized by high mortality petechial hemorrhage in proventriculus, ulcers and hemorrhage at caecal tonsils and pneumoencephalitis. It is also known as Newcastle disease (Fig.3.1).

Etiology

• Paramyxo virus

• RNA virus-3 types

• Lentogenic- mild virulent

• Mesogenic- moderate virulent

• Velogenic- highly virulent

Salmonellosis

Salmonellosis is caused by G-bacteria Salmonella and characterized by early chick mortality with necrosis and hemorrhage in liver, heart and spleen and enlarged bronze coloured liver with focal or diffuse necrosis, marbled spleen and/or oophoritis and salpingitis in adults (Fig.4.1).

Etiology

• Salmonella enteritidis var. Galinarum (S. Galinarum).

• G-, rod, non lactose fermenter.

• White small colonies on MLA and pink on BGA.

Chronic Respiratory Disease (CRD)

Chronic respiratory disease is caused by Mycoplasma gallisepticum and E.coli in birds under poor management and characterized by cloudy air sacs with thickening of their wall, accumulation of cheesy material in air sacs and lungs (Fig.6.1).

Etiology

• Mycoplasma gallisepticum

• E.coli

• Bad management like poor ventilation and inclement weather conditions

• Disease occurs more during winter and rainy season.

Spirochetosis

Spirochetosis is caused by a spiral shaped organism Borrelia anserina and characterized by greenish diarrhoea, enlarged and mottled spleen, small necrotic patches on liver and linear haemorrhage in proventriculus. This disease is transmitted by a tick Argas persicus and thus also known as “tick fever” or “tick paralysis”.

Etiology

• Spirochete Borrelia anserina

• Spiral shape 8-24m long and 0.2 to 0.3 m wide microorganism having 8-11 spirals.

• Stained with aniline dyes

Roundworms (Nematodes)

1. Ascaridia galli

A. galli is the common roundworm of poultry seen in intestines at the time of necropsy. This parasite may cause retardation of growth, loss of egg production, catarrhal enteritis and sometimes mortality due to obstruction of gut (Fig.9.1).

Etiology

• A. galli parasites are grey, thread like 5-10 cm in length.

• Eggs are elliptical with thick and shiny wall.

Pathogenesis

Transmission occurs through faecal - oral route. The eggs may survive for several months in litter and they take at least 10-15 days to develop the infective stage. The eggs of parasites hatch in proventriculus or intestine to develop fully grown parasite within 40-50 days.

Cannibalism

Cannibalism is a bad habit of birds in which the birds attack their fellow birds and eat their flesh. It is done through sharp end of beak, which causes deep wounds specially on vent. Sometimes the wounds are so deep that leads to death of affected bird. Two types of cannibalism vices are common i.e. vent pecking and feather pecking.

Cause / Predisposing Factors

• Over crowding

• Genetic predisposition

• Hemorrhage in external genitalia

• Protein deficiency (arginine and methionine deficiency)

• Loss of feathers • Wounds

• Prolapse of cloaca

Introduction

In simple terms, business refers to buying and selling of products. Business would define itself as an organized effort through companies aimed at supplying consumers with products and services in a return for monetary compensation. However, this simplistic definition is insufficient to assess the impact of the environment on modern international business operations. For easy understanding, modern business can be explained as a complex field of industry and commerce involving activities associated with both production and distribution. These activities cater to the wants and desires of society at one extreme and provide profit for business firms at the other.

Indian leaders, particularly the first Prime Minister Dr. Jawaharlal Nehru, who had conceived the theory of five year plans, had regarded high economic growth and higher income among poor population as the foremost objectives before the newly independent nation. To achieve these objectives, government was made to play a very important role, and since 1951, a series of five year plans have been the driving forces behind the economic prosperity of the country. Despite of experiencing growth in 1950s, it was still lower than that of many Asian countries. Between 1951-79, the Indian economy had grown at an average rate of 3.1 percent a year at constant prices. Growth in the industrial sector had been about 4.5 percent a year, while for the agriculture sector, growth was 3.0 percent over that period. Many of the political leaders associated with the movement were also in support of a socialistic development pattern. They favored state interference in the economy. They encouraged state ownership of core industries.

Introduction

Globalization, as defined by the International Monetary Fund (IMF), refers to the increasing economic interdependence among nations, characterized by rising volumes and diversity of cross-border transactions in goods and services, international capital flows, and the more rapid dissemination of technology worldwide.

Key Characteristics of Globalization

1. Expansion opportunities for businesses globally.

2. Elimination of distinctions between domestic and international markets.

3. Freedom to buy and sell products and services across any country.

4. Production and distribution facilities are established based on economic feasibility at optimal locations worldwide, rather than national priorities.

5. Product planning and development are influenced by global market conditions.

6. Sourcing raw materials from across the globe to maximize efficiency.

7. Global strategies, organizational structures, cultures, and management practices.

8. Viewing the world as a unified market.

Concept of Entrepreneurship

Entrepreneurship can be defined as the propensity of mind to take calculated risks with confidence to achieve a pre-determined business or industrial objectives. That points out the risk-taking ability coupled with decision making. The word ‘entrepreneurship’ typically means to undertake. It owes its origin to the western societies. But even in the west, it has undergone changes from time to time. In the early 16th century, the term was used to denote army leaders. In the 18th century, it was used to denote a dealer who buys and sells goods at uncertain prices. Towards 1961, Schumpeter, used the term innovator, for an entrepreneur. Two centuries before, the concept of entrepreneurship was shady. It is only in the recent years that entrepreneurship has been recognized widely all over the world like in USA, Germany, Japan and in the developing countries like ours. Gunnar Myrdal rightly pointed out that Asian societies lack entrepreneurship not because they lack money or raw materials but because of their attitudes. Till recently, in the west, the entrepreneurship is mainly an attribute of an efficient manager. But the success achieved by entrepreneurs in developing countries demolishes the contention that entrepreneur is a rare animal and an elusive character. In India the definition of an entrepreneur being the one who undertakes to organize, own and run a business has been accepted in a National Seminar on entrepreneurship organized in Delhi in 1975. Still there has been no consensus on the definition of entrepreneurship and qualities of entrepreneurship.

Introduction

Entrepreneurship has become a major driver of economic development in recent years. It involves starting new businesses or revitalizing existing ones, often through recognizing and seizing new opportunities. Entrepreneurs are individuals who organize, manage, and take on the risks of a business venture with the aim of making a profit. They identify market opportunities and introduce new technologies and organizational improvements. Entrepreneurs constantly innovate and create value from these opportunities. According to Hisrich (1990), entrepreneurs are proactive and creative individuals who can organize resources and manage risks and failures to succeed.

To grow as an entrepreneur, it is essential to develop and refine key entrepreneurial traits. These traits shape an entrepreneur’s skills and abilities, aligning with their motivations and guiding them to drive their business forward. Entrepreneurial characteristics are a mix of personal, social, and environmental factors that influence a business ecosystem. These traits play a key role in shaping a company’s goals and strategies, ultimately affecting its performance.

Introduction

Meaning of Entrepreneur

The term “entrepreneur” is derived from the French verb entreprendre, which means “to undertake.” Historically, its meaning has evolved. In the early 16th century, it referred to individuals involved in engineering tasks like construction. By the 18th century, the term came to describe those engaged in economic activities. Over time, the concept of an entrepreneur shifted from someone participating in military ventures to someone actively involved in business and economic pursuits. Today, an entrepreneur is defined as an individual who innovates, organizes resources, and takes on risks to create new ventures or products with the goal of generating profits.

Richard Cantillon was the first who proposed the term “entrepreneur is an agent who buys factors of production at certain prices in order to combine them into a product with a view to selling it at uncertain prices in future.”

Introduction

Entrepreneurship, known for its ability to drive innovation, economic development, and societal change, is a dynamic force that plays a crucial role in global advancement. However, the path of an entrepreneur is not a straightforward journey from concept to achievement. Instead, it is a complex process influenced by numerous factors, which together form the entrepreneurship development cycle. Gaining a deep understanding of this cycle, along with the entrepreneurial environment and the challenges entrepreneurs face, is essential for cultivating a thriving entrepreneurial ecosystem.

The entrepreneurship development cycle refers to the various stages that entrepreneurs go through as they turn ideas into successful ventures. The cycle starts with ideation, where entrepreneurs identify opportunities and develop innovative solutions to address unmet market needs or problems. This is followed by a feasibility assessment, during which entrepreneurs assess the potential of their ideas, considering market demand, competition, and the resources needed. After confirming the viability of their concepts, entrepreneurs move on to resource acquisition, gathering the capital, talent, and infrastructure necessary to bring their ideas to life. The subsequent phases include launching the venture, establishing a market presence, and pursuing growth through strategic decision-making and adaptability to shifting market conditions.