eChapters

1.     Plant Tissue Culture Laboratory
    
2.     Cleaning and Sterilization of Glass Wares
    
3.     Media Sterilization
    
4.     Media Composition and Media Preparation
    
5.     Callus Culture in Carrot
    
6.     Culture of Shoot Tip (Vanilla)
    
7.     Anther Culture of Datura
    
8.     Subculture of Carrot Callus
    
9.     Hardening of Plantlets
    
10.     Isolation of Protoplast from Plant Leaves

1.     Isolation of Plant DNA
    
2.     RAPD Analysis of Genomic DNA
    
3.    Agro Bacterium based Transformation in Plants
    
4.     Assay for Gus Operon
    
5.     Confirmation of Transformation using Polymerized Chain Reaction (PCR)
    
6.     Primer Designing
    
7.     Isolation, Purification and Identification of Plant Secondary Metabolites
    
8.     Evaluation of Antioxidant Activity of Plant Extract
    
9.     Estimation of Total Phenolic Content in A Plant Extract
    
10.     Estimation of Total Flavanoid Content

1.     Animal Biotechnology Laboratory
    
2.    Growing Cells with Serum-Basal Medium Eagle (BME), MEM
    
3.     Media Preparation and Sterilization
    
4.     Contaminations in Animal Tissue Culture

1.     Tissue Collection and Culture
    
2.     Trypsinization of Cells
    
3.     Cell Separation by Density Gradient Method
    
4.     Low Speed Separation of Cells
    
5.     Isolation of Lymphocyte
    
6.     Cryo Preservation
    
7.     Isolation of Spleen Cells
    
8.     Isolation of Peritoneal Macrophage
    
9.     Isolation of Blood Dna
    
10.     Differential Count of Leucocytes
    
11.     Slide Gel Electrophoresis
    
12.     Haemagglutination Assay
    
13.     Elisa
    
14.     Western Blotting
    
15.     Widal Test
    
16.     Identification of Y-chromosomes in Cattle using Pcr

1.     Maintaining Embryonic Stem (ES) Cells
    
2.     Establishing ES Cells
    
3.     Freezing of ES Cells
    
4.     Thawing of Frozen ES Cells
    
5.     Production of Transgenic using ES Cells
    
6.     MTT Assay for Cell Viability

1.     Isolation and Purification of DNA from E.coli
    
2.     Isolation of Plasmid DNA
    
3.     Gel Electrophoresis of DNA
    
4.     Southern Blotting
    
5.     Restriction Digestion

1.1. INTRODUCTION

During the last 30 years, India’s food production nearly doubled from 102 million tons. Almost all of this increase resulted from higher yield in all food grains rather than increase in area, particularly wheat, rice, maize and some extent in pulses and oil seed crops. This has contributed to a significant reduction in poverty from 55% to 26% (Dar, 2011). The increase in yield is the result of scientific innovations in India and other parts of the world and their adoption in India.

In 1871, the Government of India created Department of Agriculture. The impact of these departments in enhancement of crop productivity was not remarkable. Later, the Government of India established the Imperial Agricultural Research Institute in 1905 in Pusa, Bihar to enhance the agricultural research and education. Later, the headquarter of Imperial Agricultural Research Institute has been shifted to New Delhi in the year 1936. The name of the institute has been changed in 1946 as Indian Agricultural Research Institute. To boost the agricultural Research activities, Imperial Council of Agricultural Research was established in the year 1929, subsequently, the name of Imperial Council of Agricultural Research has been changed to its present form of Indian Council of Agricultural Research (ICAR) in 1946. Since then, ICAR playing significant role in all sphere of agricultural research, education and extension. A number of varieties and hybrids of different crops have been developed and released by the scientists of ICAR. To test the outcome of the agricultural research, ICAR has started All India Coordinated Crop Improvement Projects, which provide an efficient channel for multilocation testing of newly evolved strains.

2.1. INTRODUCTION

Pollination is the process of mating in plants, that is, the transfer of pollen from anther to stigma. In flowers, pollen delivered to the stigma through a wide range of mechanisms that ensure an appropriate balance in the genetic makeup of the species. Most plant species have evolved ways to ensure an appropriate degree of interchange of genetic material between individuals in the population, and cross-pollination is the normal type of pollination. Plants benefit most by being pollinated by other individuals because this broadens the genetic characteristics of individual plants. As a result, they are more adaptable to necessary changes. Self- and often-pollinated crops evolved from cross pollinated crops.

Pollination followed by fertilization is important in agriculture, particularly for the crops whose economic part is either fruit or seed, such as, rice, wheat, fruit vegetables etc. For other crops, these events are also important for production of seed for cultivation of next generation, for examples, green vegetables (amaranth, palak, cabbage, cauliflower etc).

The study of pollination brings together many disciplines, such as botany, plant breeding, horticulture, entomology, and ecology. The pollination process as an interaction between flower and vector was first addressed in the 18th century by Christian Konrad Sprengel. It is important in horticulture and agriculture, because fruiting is dependent on fertilization, which is the end result of pollination.

3.1. INTRODUCTION

Heterosis is the superiority of the F1s over the parental forms. The term heterosis was first used by Shull (1910) to describe the interpretation of increased vigour, size, fruitfulness, speed of development, resistance to diseases and insect pests or some other characteristics manifested by out-breeding organisms as compared to corresponding inbreds. Exploitation of heterosis is gaining momentum due to increased yield, quality, uniformity, better desirable traits and management of biotic stresses. The future of most of the economic crops will be for hybrids for sustainable development of higher economic returns to the farmers. Sustainable increase in area under hybrid crops is witnessed by its popularity and adoption by the farmers.

4.1. INTRODUCTION

Rice (Oryza sativa) is the major food crop in the world. Nearly 40% of the world population consumes rice as the major staple food. Most of the people, who depend on rice as primary food, live in the less developed countries.  Archeological evidence on rice in India dates back to 1500-1000 B.C. Since the dawn of civilization, rice has served humans as a life-giving cereal in the humid regions of Asia and, to a lesser extent, in West Africa. Introduction of rice into Europe and the America has led to its increased use in human diets. There are 42 rice producing countries throughout the world but China and India are major rice production centers. Rice is grown in wide range of agro-climatic conditions ranging from mountainous (Jammu) lands to low land delta areas (Sundarban), spanning an area from 53° latitude north to 35° south of the world but about 90% of the crop is grown and consumed in Asia. Rice provides fully 60% of the food intake in Southeast Asia and about 35% in East Asia and South Asia. The highest level of per capita rice consumption (130-180 kg per year, 55-80% of total caloric source) takes place in Bangladesh, Cambodia, Indonesia, Laos, Myanmar (Burma), Thailand, and Vietnam (Kenneth and Kriemhild, 2000). In many cultures of the world rice is the central part of people’s life and culture. Rice is an excellent food and is an excellent source of carbohydrates and energy.

5.1. INTRODUCTION
     
Rice is an important provider of nourishment for the world’s population. Indian scented rice, ‘Basmati’ has dominated the domestic and international market for aroma. Aromatic rice is an important commodity worldwide and command premium price over non-scented varieties. Most of the trade in aromatic rice is from India, Pakistan and Thailand. Bulk of aromatic rice from India and Pakistan consist of Basmati, while Thailand is the supplier of Jasmine rice. The Indian subcontinent has the Natural Gift of basmati rice that has been accepted as the best scented, long and slender grain in the world markets and gets high price. In addition to Basmati, many local landraces are grown traditionally, which excel in aroma, grain quality and cooking quality. Most of these genotypes are short bold to medium bold grain and hence not traded in the international market. However, in the domestic market its demand is greater than the Basmati rice. These are mainly used for special preparation, such as Khir in Bengal.

6.1. INTRODUCTION

After introduction of TN-1 (Taichung Native-1), Jaya, IR8 and other high yielding rice varieties during sixties and seventies, the productivity increased drastically and remained at plateau since last three decades. With no possibility to extend land area for rice cultivation in Asia, there is some scope for production advantages in rice through further expansion of high yielding varieties coverage in Pakistan, Thailand and India, expansion of area under irrigation in Bangladesh, Cambodia, India, Myanmar, the Philippines, Thailand and Vietnam, or expansion of boro rice in Bangladesh, India and Myanmar. Thus, utilization of heterosis has become one of the main methods for increasing yield. Hybrid is one of the viable and proven technologies to improve rice productivity. China is the first successful country to produce hybrid rice in appreciable quantity. This technology not only helped China to increase the rice productivity, but also to save land for crop diversification. The 'National Food Security Mission' has been launched envisaging increase of annual rice production by 10 million tons by 2011-'12. Here, hybrid rice may play important role to achieve the assigned target.

7.1. INTRODUCTION

The cultivation of wheat (Triticum spp.) reaches far back into history. Wheat was one of the first domesticated food crops and for 8000 years has been the basic staple food of the major civilizations of Europe, West Asia and North Africa. Today, wheat is grown on more land area than any other commercial crop and continues to be the most important food grain source for humans. Its production leads all crops, including rice, maize and potatoes.

Wheat is special in several ways. It is grown on more than 240 million ha, larger than for any other crop, and world trade is greater than for all other crops combined. The raised bread loaf is possible because the wheat kernel contains gluten, an elastic form of protein that traps minute bubbles of carbon dioxide when fermentation occurs in leavened dough, causing the dough to rise (Hanson et al., 1982). It is the best of the cereal foods and provides more nourishment for humans than any other food source. Wheat is a major diet component because of the wheat plant’s agronomic adaptability, ease of grain storage and ease of converting grain into flour for making edible, palatable, interesting and satisfying foods.

8.1. INTRODUCTION

Exploitation of heterosis through development of hybrid wheat is one of the most attractive options to increase wheat productivity. The efforts on developments of hybrid wheat started in early fifties (Kihara, 1951; Fukasawa, 1953) through cytoplasmic male sterility system (Livers and Heyne, 1968). Later, in seventies, research works from public as well as private sectors have shifted their efforts to the use of gametocide (chemically hybridizing agent-CHA), and a number of gametocides were tested in eighties. In wheat, heterosis is especially interesting for grain yield and for the resistance of plants to both parasites and climate risks (Rousset, 1986). With this advantage in mind, the first phase of hybrid wheat development started in the United States in 1975. Chronological events of wheat hybrid development have been given in Table 8.1. Subsequently, a number of hybrid wheat has been released in countries such as Argentina, France, and United States (Pickett, 1993).

9.1. INTRODUCTION

Maize (Zea mays L. ssp. mays 2n = 20), known as corn in some countries is an important cereal crop of the world. The scientific name from Greek word ‘Zea’ for a kind of grain and West Indian word ‘malnis’ for corn. Maize was domesticated in Mesoamerica and hence formed the Mesoamerican people’s identity and later it spread throughout American continent. Maize is widely cultivated throughout the world and a greater weight of maize is produced each year than any other cereal grain. USA produces almost half of the world’s harvest (42.5%) and other top producing countries include China, Brazil, Mexico, Argentina, India and France. During the year 2007, worldwide maize productivity was around 4970 kg per ha. Maize is the only cereal that can be harvested and used at various stages of plant development. The crop can be cultivated on a wide area from 580 N to 400 S from below sea level to altitudes higher than 3000 m and in areas with 250 mm to more than 5000 mm of rainfall per year (Shaw, 1988, Dowswell et al., 1996).

10.1. INTRODUCTION

The development of hybrid breeding methodology in maize and its successful application to exploit heterosis is considered as significant achievement of plant breeding in the present century. 
Even though Beal (1880) suggested for commercial cultivation of F1 varietal hybrids. It was Shull (1909) on the basis of his experiment gave pure line method of corn breeding, which laid foundation for present day hybrid breeding. Pure line methods of maize breeding involve three steps. 
    
·    Large scale inbreeding to obtain many homozygous/nearly homozygous inbred lines. 
    
·    Testing the selected inbred lines in all possible crosses.
    
·    Practical utilization of inbred lines in seed production of single cross hybrids.

11.1. INTRODUCTION

Sorghum [Sorghum bicolor (L.) Moench] is the fifth most important cereal crop and is the dietary staple of more than 500 million people in over 90 countries, primarily in the developing world. It is grown on 47 m ha in 104 countries in Africa, Asia, Oceania, and the Americas. Among those, USA, Nigeria, India, Mexico, Sudan, China, and Argentina are the major sorghum producers globally. Sorghum grain is mostly used directly for food (55%), and is consumed in the form of porridges (thick or thin) and flat breads; however sorghum is also an important feed grain (33%), especially in Australia and the Americas. Stover (crop residue after grain harvest) is an important fodder source to both milch and draft animals in mixed crop-livestock systems. Sorghum is also a good source of green fodder due to its quick growth and high yield and quality of biomass. Of late, sorghum with sugar-rich juicy stalks (called sweet sorghum) is emerging as an important biofuel crop. Thus sorghum is a unique crop with multiple uses as food, feed, fodder, fuel and fiber. Sorghum is generally grown in rainy season (spring) but in India it is grown in both rainy and postrainy seasons. The postrainy season sorghum grain is prized for its food value (Reddy et al., 2009).

12.1. INTRODUCTION

Sorghum [Sorghum bicolor (L.) Moench] is an important food/feed/fodder crop in more than 90 countries across the world and it is one of the cheapest sources of energy and micronutrients (Reddy et al., 2009; Ashok Kumar et al., 2011). Considerable efforts have been made to improve sorghum productivity through genetic enhancement and crop production and protection research all over the globe. Sorghum being an often cross-pollinated crop with cross pollination up to 2-15% depending on genotype and environment (House 1985), initially, pure-lines were the target products although phenomenon of heterosis was demonstrated in sorghum as early as in 1927 (Conner and Karper, 1927). Predominant self-pollinating nature of sorghum with tiny florets with single seed per emasculation and pollination prevented commercial exploitation of heterosis for commercial sorghum production. However, the discovery of a stable and heritable cytoplasmic-nuclear male-sterility (CMS) mechanism (Stephens and Holland, 1954), enabling large-scale, economic hybrid seed production gave a fillip to the exploitation of heterosis since early 1960s. The first commercial sorghum hybrid CSH 1 was released in 1964 in India. Using CMS mechanism, large number of commercial hybrids have been developed and released/marketed for commercial cultivation in all sorghum growing regions with strong Indian National Agricultural Research Systems (NARS).

13.1. INTRODUCTION

Pearl millet crop has a wide adaptability to local environments. It is hardy and can be grown in areas which are very hot and dry and on soils too poor for sorghum. Pearl millet is considered more efficient in utilization of soil moisture and has a higher level of heat tolerance than even sorghum and maize. It is adapted to warm and dry climate but most of the area under the crop is in drought- prone areas of the world. In these areas, pearl millet is usually grown with limited inputs, sparse rainfall, low soil fertility, and also face disease and pest problems, with correspondingly poor yields.
 
The low average yields are primarily a result of the hot, dry conditions where pearl millet is mostly grown, rather than pearl millet plant’s capability. Pearl millet hybrids grown under better conditions yield 3000 to 4000 kg/ha compared to 300 to 800 kg/ha when moisture and soil fertility become limiting factors. A majority of the subsistence farmers who typically cultivate this crop are unable to take advantage of high yield potential because they have limited options for improving their management practices. Pearl millet production can be increased through growing varieties/hybrids with improved tolerance to drought, diseases and responding to higher rates of fertilizer applications.

14.1. INTRODUCTION

Barley (Hordeum vulgare L. emend. Bowden) is one the most important ancient crops and played very important role in the development of agriculture and human civilization. It has also played crucial role in the progress of science of agriculture, physiology, genetics, plant breeding and malting and brewing science. It is the fourth most cereal crop after rice, wheat and maize in acreages and production in the world.  It is grown and used all around the world in many countries and has fed livestock, poultry and people since ancient time.  Barley was domesticated about 10,000 years ago in the Fertile Crescent of the Near East. Fertile Crescent is considered the first of at least seven primary centres of agriculture origin in the world (Smith, 1998; Baik and Ulrich, 2008). Barley is best known around the world as a feed grain and a premier grains for malting and brewing. When most people think of composition of beer, they only think of barley over other cereals. Although utilization of barley for food is relatively low as compared to other cereals today, throughout its history barley has remained an important and major food source poor people living in Western and Eastern Asia and Northern and Eastern Himalayan region nation like Tibet, Nepal and in Northern and Eastern Africa (Newman and Newman, 2006, 2008). Moreover, there has been resurgence of interest and use of barley as food primarily in the developed world due to an increasing emphasis on incorporating the diversity in consumption of whole grain in people’s diets due to its health benefits.

15.1. INTRODUCTION

Finger millet Eleusine indica (L.) is an Old World cereal grass commonly known as ‘finger millet’, is one of the staple food crop especially in India and Africa, which is widely grown in the arid regions of Asia and Africa and important minor millet grown in India.  Eleusine, the generic name, is obtained after the “Eleusine” the Greek Goddess of cereals and the common name finger millet is derived from the finger like branching of the panicle. It is also know by Goosegrass, Yardgrass, Wiregrass, Silver Crabgrass, Crowfoot Grass. It is commonly known as Finger millet in English, Kelvaragu in Tamil, Nachani in Marathi etc. Some Indian varieties of finger millet are Kempu ragi, Ragalli shivalli, Pichakaddi etc.  This crop is one among the hardiest crops which can with stand severe drought conditions and can also grow well under scanty rainfall condition. However with good irrigation facilities the yield of finger millet matches with crops like rice. Finger millet seeds remain free of pests and insects for a long duration extending up to 50 years hence serving as a food reserve in case of tough situations like famine.

16.1. INTRODUCTION

Millets are a large part of the basic diet for farm households in the world's poorest people in the poorest countries. The production of millets on average for the 5 years have been given in Table 16.1. Top consuming countries are India, Nigeria, Niger and China. However, just because a country is a top global consumer does not mean that millet is a significant source of calories for them. India and China are rank as the 1st and 4th consumers of millet, but due to their population size they rank 11th and 38th in per-capita consumption, accordingly.

Millet particularly the finger millet) is an excellent dietary source of calcium, iron, manganese, and methionine-  an amino acid lacking in the diets of hundred of millions of the poor who live on starchy foods such as cassava, plantain, polished rice, and maize meal. Millet use is diverse, including in cereals (including porridge and kasha), soups, breads and stuffings, fermented beverages, and baby food.

17.1. INTRODUCTION

Yam bean is one of the adaptable, low-maintenance, and nutritious food crop grown in moderate soil. It has many synonyms, in India it is known as Mishrikand (Hindi), Keshar or Ram-kaseru or Shankalu (Bengali). The root crops produced by legumes have long recognized as important and the FAO (1979) has recommended them as a source of human nutrition. The tuber is rich in starch (15-20%), 3-4% sugar, and have considerable amount of protein (2-3%). The harvested crop keeps well for months under ambient temperature. Kale (2006) observed relatively high iron contents in storage roots (up to 130 mg/kg of storage root dry matter). It can also be used for alcohol production.

The crop is very hardy, resistant to common insect pests and diseases. Most important is that the crop is not grazed neither by goats nor cows. The crop grows well in hot-humid environment and requires long, warm frost-free growing season. A moist, light textural soil is preferred for production of tuberous roots. As a legume crop, it can be grown without nitrogen fertilizer. Under good growing condition its root nodules habour symbiotic colonies of nitrogen-fixing bacteria- Rhizobium spp. (Nielsen et al., 1998b). A season crop of yam beans can release as much as 53 tons of usable nitrogen per acre into the soil. Mycorrhyzae also associated with the crop, thus facilitating phosphorus uptake. Thus, yam bean is highly suited to the needs of small farmers, and from both an ecological and a socioeconomic perspective

18.1. INTRODUCTION

Plant genetic resources (PGRs) are the foundation for the development of a food and nutritionally secured society. Food is the foremost important to sustain life and livelihood followed by shelter and cloth. Thus, the slogan of 3Fs may be modified as, “Food, Frame (shelter) and Fibre (cloth)”. All these 3Fs we can get from PGRs. There must be balance between demands and resources. Gradually the demand for basic needs increasing. So, we have to improve the PGRs to cope with the increasing demands. PGRs are the foundation for the development of these 3Fs. India is a country rich in crop biodiversity and genetic resources. The status of Germplasm accessions in the National Gene Bank is being presented in Table 18.1.

Key Facts
    
·    The United Nations Framework Convention on Climate Change (UNFCCC) defines ‘climate change’ as a ‘Change of climate which is attributed directly or indirectly to human activity that alters the composition of the global atmosphere and which is in addition to natural climate variability observed over the comparable time periods’.
    
·    The main green house gases (GHGs) that have caused the global climate change or global warming are carbon dioxide (CO₂), methane (CH₄) and nitrous oxide (N₂O).
    
·    CO₂ is the principal anthropogenic GHG that affects the earth’s radiative balance. It is the reference gas against which other GHGs are measured and therefore has a Global Warming Potential (GWP) of 1. CH₄ and N₂O have a GWP of 21 and 310 respectively.
    
·    CO₂ is responsible for most of the enhanced green house effect of more than 50%, CH₄ for about 10-15% and N₂O for about 8%.
    
·    The global atmospheric concentrations of CO₂, CH₄ and N₂O have increased since 1750. While CO₂ increased from a pre-industrial value of 280 ppm to 379 ppm in 2005, CH₄ increased from 715 ppb to 1774 ppb and N₂O increased from 270 ppb to 319 ppb during the same period.

Key facts

Agriculture
    
·    Agriculture production (plant growth) is highly dependent on climate since crop growth is influenced by solar radiation, temperature and precipitation and sensitive to climate variability and weather extremes (droughts, floods, severe storms).
    
·    Climate change is projected to have an effect on local agriculture and the net result could be harmful (e.g. frequent droughts, salinization of agriculture land due to sea level rise) or beneficial, (e.g. enhance CO₂ and higher yield, longer growing season, increased precipitation) or mixed.
    
·    Climate change or a doubling of GHGs (2 x CO₂) is projected to cause both gains and losses of agriculture land.
    
·    Each crop has a specific temperature range at which vegetative and reproductive growth will proceed at the optimal rate and exposures to extreme temperatures during these phases can impact growth and yield. Higher temperatures during the reproductive stage of development can affect pollen viability, fertilization and grain or fruit formation. 
    
·    Warming generally allow plants to grow faster that are below their optimum temperature, however for cereal crops faster growth means there is less time for the grain itself to grow and mature, reducing yields. 
    
·    Night-time temperatures are rising faster than daytime temperatures and are expected to continue rise in the future. According to International Rice Research Institute (IRRI), Philippines, the rise of night time temperature decreases rice yield by 10% for each 1°C increase in growing-season night time temperature in the dry season.

Key Facts
        
Agricultural food
    
·    There are multiple pathways through which climate-related factors may impact food contamination and food safety, food quality, foodborne diseases including changes in temperature and precipitation patterns, increased frequency and intensity of extreme weather events, ocean warming and acidification and changes in the transport pathways of complex contaminants.
    
·    All or most foodborne pathogens and their associated diseases are linked to changing environmental conditions, the notable of which are campylobacteriosis, salmonellosis and vibriosis.
    
·    An estimated 30% of reported cases of salmonellosis across much of continental Europe have been attributed to warm temperatures, especially when they exceed a threshold of 6°C above average. Likewise both in Australia and Canada a positive correlation was found between increasing temperature and incidence of salmonellosis.
    
·    Zoonosis such as Rift Valley Fever (RVF), West Nile Fever, tick-borne diseases and non-zoonotic animal diseases such as Blue tongue, African horse sickness, African swine fever are examples of animal diseases whose distribution is expected to be strongly influenced by climate change and variability.
    
·    Mycotoxin is a toxic secondary metabolite produced by filamentous fungi, commonly known as moulds and may contaminate agricultural commodities (food) by growing on them both before and after harvest, whenever the humidity and temperature are permissive. The most prominent mycotoxins are aflatoxins, deoxynivalenol, zearalenone, ochratoxin-A, fumonisin, and patulin.
    
·    The humans ingestion of mycotoxins occur through consumption of the mycotoxin contaminated plant-based foods, such as grains, maize, barley, rice, coffee, nuts and their residues, and metabolites in animal-derived foods, for example aflatoxin M1 (AFM1) in milk and meat products. In addition, mycotoxins may occur in beer and wine.

Key facts
    
·    The Food and Agriculture Organization (FAO) defines food security as a ’situation that exists when all people, at all times, have physical, social and economic access to sufficient, safe and nutritious food that meets their dietary needs and food preferences for an active and healthy life’. There are four dimensions of food security: Food Availability (availability of sufficient food for all people at all times, i.e. production); Food Accessibility (physical and economic access to food at all times, i.e. affordability), Food Utilization (access to food that is nutritious, safe and produced in environmentally sustainable ways, i.e. nutrients in food and food safety); and Food Stability (reliability of adequate food supply).
    
·    Agriculture is important for food security since it provides food for the people and is the primary source of livelihood for 36% of the world’s total workforce. In the heavily populated countries such as Asia and the Pacific, about 40% to 50%, and in sub-Saharan Africa, about two-thirds of the working population make their living from agriculture.
    
·    Agriculture, livestock and fisheries production are all sensitive to climate and are affected by its changes. Climate change will increase hunger and malnutrition, emergence of new pests and diseases and will threaten fishing and aquaculture industries.

Key facts
        
Agriculture and livestock
    
·    The Intergovernmental Panel on Climate Change (IPCC) defines adaptation as ‘adjustment in natural or human systems in response to actual or expected climatic stimuli or their effects, which moderates harm or exploits beneficial opportunities’. The adaptation can be considered as tackling the effects of climate change, whereas mitigation is tackling the cause of climate change such as reducing the sources or enhancing the sinks of greenhouse gases. There could be different types of adaptation such as autonomous (spontaneous) and planned adaptation (policy decision).
    
·    Autonomous adaptation includes altering varieties and/or species those are more appropriate to the altered thermal regime or with increased resistance to heat shock and drought; altering the timing or location of cropping activities; altering fertilizer rates to maintain grain or fruit quality consistent with the climate and altering amounts and timing of irrigation and other water management practices; diversifying income by integrating other farming activities such as livestock raising, integrated agriculture and aquaculture/fish farming; development and use of varieties and species resistant to pests and diseases, wider use of technologies to ‘harvest’ water, conserve soil moisture (e.g. crop residue retention) and to use water more effectively in areas where rainfall is projected to decrease and using seasonal climate forecasting to reduce production risk.

Key Facts
    
•    The United Nations Framework Convention on Climate Change (UNFCCC) defines ‘climate change’ as a ‘Change of climate which is attributed directly or indirectly to human activity that alters the composition of the global atmosphere and which is in addition to natural climate variability observed over comparable time periods’.
    
•    The main green house gases (GHG) that have caused the global climate change or global warming are carbon dioxide (CO₂), methane (CH₄) and nitrous oxide (NO₂).
    
•    CO₂ is the principal anthropogenic greenhouse gas that affects the Earths radiative balance. It is the reference gas against which other GHGs are measured and therefore has a Global Warming Potential (GWP) of 1. CH₄ and N₂O have a GWP of 21 and 310 respectively.
    
•    CO₂ is responsible for most of the enhanced green house effect of more than 50%, CH4 about 10 to 15% and NO2 about 4%.
    
•    The global atmospheric concentrations of carbon-dioxide, methane and nitrous oxide have increased since 1750, CO₂ increased from a pre-industrial value of 280 ppm to 379 ppm in 2005, CH₄ increased from 715 ppb to 1774 ppb and NO₂ increased from 270 ppb to 319 ppb in 2005.

Key facts

    
•    Though water is the most widely occurring substance on earth, freshwater accounts for only 2.5% whereas the rest of water is saltwater. The agriculture sector uses more than 75% of freshwater available. 
    
•    The current observed climate change impacts on water resources include increased precipitation at high latitudes and temperate areas, decreasing precipitation at low latitudes; decreasing snow covers, and glaciers in most regions; decreasing flows in some river basins; floods and droughts in some countries and significant decreases in lake and river size in Africa.
    
•    A number of climate models projected that a doubling of atmospheric concentrations of CO₂ would increase global precipitation by at least 5%.  The precipitation is expected to increase in some areas, such as, at high latitudes and in the tropics (south east monsoon regions and over the tropical Pacific), whereas the precipitation is likely to decrease in the sub-tropics (e.g. much of North Africa and northern Sahara).

Key facts

    
•    Agriculture is the major land use across the globe. Agriculture production (plant growth) is highly dependent on climate since crop growth is influenced by solar radiation, temperature and precipitation. Agriculture is also sensitive to climate variability and weather extremes (droughts, floods, severe storms).
    
•    The yields of C₃ crops (rice, wheat, and soybean) is expected to increase (10-20%) with predicted doubling of CO₂ concentrations compared to C₄ crops (maize, sorghum, millet, sugarcane) since C₃ plants respond readily/positively to increased CO₂ levels. Therefore, CO₂ enrichment may benefit temperate and humid tropical agriculture compared to semi-arid tropics.
    
•    The predicted rise of temperature could impact crop and horticulture yield if it exceeds optimal temperature range. For example, low latitudes crops are being grown near the limits of temperature tolerance and global warming may put them to higher stress.
    
•    On a world wide basis, global warming would benefit the mid to high latitude zones for agriculture (for a small change of temperature of 1-3^ºC), whereas low latitudes, semi-arid and tropical areas will have much reduced crop and livestock yields. However, adaptation measures may reduce such impacts.

Key facts

    
•    Fish is an important source of animal protein for billions of people (estimate 2.6 billion people). About 200 million people and their dependants worldwide, mostly in developing countries, live by fishing and aquaculture. 
    
•    Fish living in temperate and Polar Regions could be benefited from climate changes since an increase in water temperature would extend the fish growing season. Moreover, rising temperature could reduce over-wintering stress normally experienced by temperate fishes.
    
•    Rise of temperature may cause a decrease in dissolved oxygen (DO) or hypoxic conditions (as temperature and DO are inversely related) and can impair fish reproduction and may cause endocrine disruption in fish (increase incidence of malformations and may alter sex differentiation and sex determination in fish).
    
•    Bioavailability and toxicity of common pollutants such as pesticides and heavy metals may be enhanced with increasing temperatures. Therefore, exposure of toxicants at elevated temperatures may cause a decline in recreational and commercial fisheries.

Key facts

    
•    The climate change is likely to affect millions of people both directly (via increased deaths, disease and injury from heatwaves, floods, storms, fires and droughts) and indirectly (through changes in the water, air, food quality and quantity and ranges of vectors, such as mosquitoes, water-borne pathogens).
    
•    Recent observed climate change related effects include: (a) an increase in heat related deaths across the globe (Australia, Canada, France, Germany, the Netherlands, Portugal, Switzerland, UK and USA; (b) spread of vector- and rodent-borne diseases such as malaria (Africa, India) and dengue and (c) increase in food poisoning (New Zealand).
    
•    Heatwaves can cause adverse health effects such as heat cramps, fainting, heat exhaustion and heat stroke. Human deaths due to heatwaves are associated with cardiovascular and respiratory diseases and in 2003 in Europe 70,000 deaths were recorded from heatwaves alone.
    
•    Projections : (a) cold temperature is expected to become less frequent, while heat waves will become more frequent; (b) increases in heat related deaths and illness across the globe including Australia, Germany, Portugal, UK, and USA;  (c) malaria zone may expand in Australia, Africa (highlands), India and  260-320 million more people will be affected by malaria by 2080;  (d) dengue area is likely to increase as climate would be more suitable and by 2085, about 6 billion people will be at risk of contracting dengue fever including Australia and New Zealand; (e) diarrhoeal disease may increase with temperature increase; and (f) cholera outbreaks may rise as a result of more plankton blooms providing nutrients for Vibrio cholerae.

Key facts

    
•    The use of chemicals has many benefits, however, there are potential risks that some of these chemicals may cause harm to organisms and human health if they enter into the environment.
    
•    Harmful chemicals are those chemicals which are persistent, bio-accumulative, as well as toxic (PBT). 
    
•    Environmental or ecological risk assessment (ERA) consists of hazard identification, effect assessment, exposure assessment, risk characterization, risk classification, risk-benefit analysis, risk reduction and monitoring.
    
•    The five types of environmental monitoring are: chemical monitoring, bio-accumulation monitoring, biological effect monitoring, health monitoring and ecosystem monitoring. 
    
•    An integrated monitoring program is a coordinated monitoring activity comprising of both chemical and biological measurements in a variety of environmental compartments. There are four different monitoring levels in an integrated ERA, these are: suborganismals (biomarkers), organisms (bioassays), populations (bio-indicators) and ecosystems (ecological indicators).

Key facts

    
•    Arsenic (As) is a metalloid, with properties intermediate between those of a metal and a non-metal. Arsenic can exit in the environment and biological systems in various inorganic and organic forms (species). These are arsenite - AsIII, arsenate - AsV, monomethyl arsonic acid (MMAA), dimethyl arsinic acid (DMAA), arsenocholine, arsenobetaine and arsenosugars. 
    
•    Arsenicals have a wide range of toxicity. In general, inorganic arsenicals are more toxic than oragnoarsenicals to biota and trivalent arsenic (arsenite) is more acutely toxic than pentavalent (arsenate) form in the following orders:
    
    Arsenous acid (AsIII) ®  arsenic acid (AsV) ® MMAA ®DMAA ® arsenocholine ® arsenobetaine.
    
•    Under oxidizing and aerobic conditions, the predominant form of arsenic (As) in soil and water is arsenate (AsV), whereas under anaerobic and reducing/or waterlogged conditions, arsenite (AsIII) predominates.
    
•    Inorganic AsIII and AsV are the major As species in groundwater, soils, terrestrial plants, whereas the main species found in marine animals is Arsenobetaine (AsB), which is considered to be non-toxic.
    
•    The LD50 values of different arsenicals show that arsenite (AsIII) is most toxic (LD50 range 15-42 mg/kg) and arsenobetaine is least toxic (LD50 > 10,000 mg/kg).

Key Facts

    •    The heavy metals (HM) or trace metals in an aquatic environment can come from natural (e.g. geological minerals, wind blown silicate dust, volcanic emissions, sea spray and combustion) and anthropogenic sources (e.g. mining, industry and sewage treatment discharges as well as electronic waste and agriculture fertilisers). In aquatic systems, the heavy metals of greatest concerns are cadmium, copper, lead, mercury and zinc.
    •    Metals may exist in a number of physico-chemical states in aqueous systems such as particulate matter and dissolved forms. Metal speciation in freshwater is strongly influenced by hardness, alkalinity, pH, natural dissolved organic matter (DOM) and redox potential. 
    •    Water hardness is quantitatively related to metal toxicity in freshwater (i.e. toxicity of heavy metals decrease with increasing hardness and alkalinity; on the other hand, toxicity increases with decreasing salinity, dissolved organic matter and chelators). The uptake and toxicity of heavy metals of most metals in marine waters is low as compared to freshwater because metals are easily complexed with chloride ions.
    •    The toxicity of HMs is related to the dissolved ionic form of the metal rather than total concentration of the metal. Each different physico-chemical forms of an element has a different toxicity, for example, if the element (e.g. Cu) is absorbed on a colloidal particles it is expected to be of little or no effect on aquatic organisms, however,  if the copper is available as free Cu (II) ion, few aquatic organisms may survive.

Key facts
    
•    Pesticides are chemicals that are broadly used to protect crops, livestock and other animals and plants from pests and diseases. They are classified according to the type of pest to be controlled (algicides, herbicides, fungicides and insecticides).
    
•    Commercial production of DDT began in 1943. At that time, DDT was considered to be a wonderful invention. It was cheap to produce, very toxic to insects and much less toxic to mammals and its usage resulted in a sharp decline of mosquito borne diseases. In 1962, it was discovered that DDT was causing egg-shell thinning in bird eggs and thus was leading to the near extinction of bird species such as peregrine falcons and bald eagles.
    
•    Pesticides use has greatly helped to increase and improve the world food production. Use of pesticides also increases farmer’s profits by preventing crop losses due to pest attacks and weed infestation.

Key facts
    
•    Dioxins are the most dangerous and ‘super toxic’ chemicals so far known on the earth and three closely related families are collectively known as dioxins. These are Polychlorinated dibenzo-p-dioxin or dioxins (PCDDs), Polychlorinated dibenzofurans or furans (PCDFs) and polychlorinated biphenyls (PCBs).
    
•    2, 3, 7, 8-TCDD (or called TCDD) is the most potent of all the congeners. The toxicity equivalency factor or TEF of TCDD has been assigned as 1, while all other congeners assigned in order of toxicity ranking magnitude relative to TCDD.
    
•    There are 75 compounds/congeners in the dioxin family (PCDD), 135 compounds in the furan family (PCDF) and 209 possible compounds in the PCBs based on the possible number of chlorine atoms.
    
•    The toxicity of dioxins and dioxin-like compounds vary substantially among the different PCDDs, PCDFs and PCBs. It is generally accepted that only 29 out of the 419 congeners of dibenzo-p-dioxins, dibenzofurans and polychlorinated biphenyls are toxic to humans and other organisms. The TCDD is most toxic and registered by the WHO as carcinogenic for humans.

Key facts

    
•    Endocrine disrupting chemicals (EDCs) are micropollutants with estrogenic or androgenic activities at very low concentrations and are emerging as a major concern for water quality.
    
•    EDCs include natural and synthetic hormones (Estrone or E1, 17b-estradiol or E2, Estriol or E3, 17a-ethynylestradiol or EE2); alkylphenols (nonylphenol or NP; octyphenol or OP), phenols (bisphenol-A or BPA), phthalates (Di-n-butyl phthalate or DBP, Di-2-(ethylhexyl)-phthalate or DEHP); organotins (tributyltin or TBT); and phytoestrogens (b-sitosterol, coumestrols, genistein). 
    
•    Wastewater treatment plants, intensive livestock effluents, industrial wastewaters, landfill, paper and pulp mill effluents and antifouling paints are important sources of EDCs in the environment.

Key facts
    
•    Biotoxins are naturally occurring toxic compounds produced by some freshwater cyanobacteria (blue-green algae) and marine algal species (dinoflagellates, diatoms).
    
•    Tonnes of pharmacologically active substances are used annually in human and veterinary medicines, some of these substances are excreted (human and animal) non-metabolised or an active metabolites and enter into the environment since waste water treatment plant (WWTP) cannot degrade them.
    
•    In Europe, around 4,000 different pharmaceutically active compounds are used as human and veterinary drugs and the annual consumption is about several hundred tonnes.
    
•    European Union and the United States laws require an assessment of potential risks to the environment as part of license application processes for new medicinal products.

•    Pharmaceutical products are generally polar, water soluble and low volatile molecules, it means that they have high mobility in the environment.

Key facts

Freshwater biotoxins

    
•    Biotoxins are naturally occurring toxic compounds produced by some freshwater cyanobacteria (blue-green algae) and marine algal species (dinoflagellates, diatoms).
    
•    Cyanobacteria are now recognised as a serious water quality problem with regard to drinking water supply and recreational use.
    
•    Environmental conditions that can promote cyanobacterial/blue-green algal blooms are: excessive nutrients (phosphorus and nitrogen); warm water temperatures (15-30OC); sunlight for photosynthesis; and quiescent or stagnant water.
    
•    Outbreak of human poisoning attributed to cyanobacteria are neurotoxins (e.g. anatoxin-a, anatoxin-a(s), saxitoxins) and hepatotoxins (e.g. microcystins, nodularin) produced by some strains of the cyanobacteria including Microcystis, Anabaena, Aphanizomenon spp.

Introduction

The major environmental issue of global nature is increasing green house effect, which is warming earth. Enhanced green house effect is mainly due to increased emission of carbon dioxide, methane, nitrous oxide and CFCs. Because of the increasing concentrations of these greenhouse gases, there is much concern about future changes in our climate and direct or indirect effect on agriculture (Arora et al. 2011). Increases in atmospheric concentrations of green house gases and additional anthropogenic perturbations of the climate system affect the spatial and temporal distribution of weather regimes which contribute to shaping the diversity of agricultural activities throughout the world (Havlik et al. 2015). Consequences of changing climatic attributes such as rainfall, moisture regimes, temperature etc. affect qualitative and quantitative production of agricultural crops as well as livestock population by altering pasture quality and yield (Thornton et al. 2009). In the context of climate change mitigation, now a days adoption of climate smart agriculture work as an effective practice.

Introduction

Globally, now scholars accepted that the climate is changing and it has implications on human culture, social systems, economic stress and ecological problems (Ford et al. 2010). The IPCC advocates to search and develop local solutions for climate change adaptations (IPCC 1996; IPCC, 2001), however, it does not recognize the breadth and strength of centuries-tested community knowledge/traditional knowledge systems (Salick et al. 2009) which are available with the people of developing world, and has been for adaptation practices and strategies to extreme weather and climate change (Turner and Clifton 2009; Salick and Ross 2009). It is well recognized that with fragile ecosystems, poor economic status and with less robust technologies required to adapt climate change, marginal and poor people of developing countries are likely to be more vulnerable with the changing climate than the industrialized countries (Tompkin and Adger 2004; Agrawal, 2008).

Introduction

Agroforestry is the intentional combination of agriculture and forestry technologies to create integrated, diverse, productive, profitable, and sustainable land-use systems (Rietveld 1995). It creates complex systems with impacts ranging from the site or practice level up to the landscape and beyond (Ellis, 2004). It is one of the most conspicuous land use systems that consists of annual and perennial plants, which are often integrated with livestock. It provides ecosystem services and reduces anthropological impacts on natural forests, arrests soil degradation, enhance soil fertility in many situations and improve farm resilience (Thangataa and Hildebrand 2012; Tewari et al. 2013). Agroforestry is a land management and farming system that are not only capable of fulfilling household needs but also maintaining and improving environmental quality. Itplays a vital role in achieving integrated rural and urban development. The changing climate has potential impacts on ecosystem goods and services by means of increased variability with greater risk of extreme weather events, such as prolonged drought, storms and floods (Lindner et al. 2010). Agroecosystem and forests are the ecosystems which are the most adversely affected by climate change by means of prevalence of pests, diseases, invasive species, species endangerment and high levels of food insecurity.

Introduction

It is well known that, over 50% of requirement of food for the entire humanity is obtained from just three crops- rice, maize and wheat though 150 crops are commercialized globally. Ethnobotanically, over 7000 plants species are cultivated or harvested from the wild which represent immense agro-biodiversity. Most of these species are under exploited but have the potential for contributing to food security, health (nutritional/medicinal), income generation and environmental services. Examples include little millets which provide calcium and iron and vitamins like niacin and sulphur containing amino acids, having more fiber than rice and wheat. Some other species which have in the recent past contributed to income generation in Indian arid zone include ber, anola, karonda and gonda. Likewise the perennial grasses in this area are not only source of nutritious fodder but also the grains e.g. gramana (Panicum antidotale) are consumed during famine. They have acquired importance being alternative sources of income as also alternate life support system during crisis. They often have neutraceutical, medicinal uses or often multiple uses. Demand for traditional food in large multi-ethnic cities and metros have brought these species into sharp focus, Hence, these are being viewed now as potential candidate for diversification in the cropping system, conservation of bio-diversity, enhancing the limits of green revolution even during climate change and finally as provider of molecules for curing various ailments of men and animals.

Introduction

Low and erratic rainfall, extreme temperatures, long sunshine duration, high wind velocity, high evapotranspiration, low fertility of soil and paucity of water resources characterize hot arid region of India. Despite these hostile conditions, the region supports a large number of human and livestock population. However, the ever increasing human and livestock population and developmental activities exerts enormous pressure on natural resources and posing serious threat to sustainability of the region. In these hostile environmental conditions, several indigenous agroforestry systems are being practiced by farmers of this region (Shankarnarayanan et al. 1987). The agroforestry, which is a dynamic, ecologically based natural resource management practices that, through the integration of trees and other tall woody plants on farms and in the agricultural landscape, diversify production for increased social, economic and environmental benefits (ICRAF 2000). A number of studies have recognized the multiple benefits of agroforestry that include carbon sequestration, reducing forest degradation, biomass production, food security, income diversification, improvement and maintenance of soil biodiversity, provision of wildlife corridors, and a host of other ecological services and social benefits. In the last three decades, agroforestry has been widely promoted in the tropics as a natural resource management strategy that attempts to balance the goals of agricultural development with the conservation of soils, water, local and regional climate

Introduction

Agroforestry, the word coined in the early seventies, has made its place in all the developed and the developing countries of the world and is now recognized as an important approach to ensuring food security and rebuilding resilient rural environments (Luedeling et al. 2014).  Agroforestry applies to private agricultural and forest lands and communities that also include highly erodible, flood-prone, economically marginal and environmentally sensitive lands (Continents 1986). The typical situation is agricultural, where trees are added to create desired benefits. Agroforestry allows for the diversification of farm activities and makes better use of environmental resources (Taylor et al. 2007). Agroforestry is defined as a land use system which integrates trees and shrubs on farmlands and rural landscapes to enhance productivity, profitability, diversity and ecosystem sustainability. It is a dynamic, ecologically based, natural resource management system that, through the integration of woody perennials on farms and in the agricultural landscape, diversifies and sustains production and builds social institutions. India has been an all-time leader in agroforestry (Chavan et al. 2015).

Introduction

Agroforestry is the science of designing and developing integrated self-sustainable land management system, which involves introduction or retention of woody components such as trees, shrubs along with agricultural crops including pasture/animals simultaneously or sequentially, on the same unit of land and at the same time, to meet the ecological as well as socio-economic needs of local people. A typical agroforestry system will allow economic and ecological interactions between woody and non-woody components and also increase, sustain and diversify the total land output. Agroforestry systems are very important for the area currently under agriculture, the number of people who depend on land for their livelihoods, and the need for integrating food production with environmental services (Garrity, 2004; Makundi and Sathaye 2004). Agroforestry provides a unique opportunity to combine the twin objectives of climate change adaptation and mitigation. Although agroforestry systems are not primarily designed for carbon sequestration, there are many recent studies that substantiate the evidence that agroforestry systems can play a majorrole in storing carbon in above ground biomass (Sathaye et al. 2001;

Introduction

India is the seventh-largest country in the world, with a total area of 3,287,263 square kilometres, roughly 2.5 percent of global landmass but is home to 17.9 percent of global human population and 1/5 of the livestock. Most of this increase in human population has occurred in second half of 20th century. Similarly, India has the highest number of cattle (210.2 m), buffaloes (111.3 m) and goats (150.4 m) in the world. It ranks second in sheep (74 m), fifth in chickens (866 m) and sixth in ducks (26 m). The increased population and associated increased demand of resources has led to significant impact on land use-land cover and has consequence in negatively impacting various ecosystem services i.e., hydrology, soil conservation, wildlife support, biodiversity and environmental protection, these agroforestry systems used to provide. Agroforestry has traditionally been a way of life and livelihood in India for centuries. Now it is a modern science inviting deliberate management of trees on farms and surrounding landscape (Bargali et al. 2009;