eChapters

ABSTRACT

The present investigation was undertaken to standardize the DNA extraction methods, to optimize the PCR conditions and generate RAPD’s to study the genetic variability in chrysanthemum. Total genomic DNA was extracted from freshly emerged leaves of chrysanthemum cultivars (Flirt, Nagpur Red, Snow Ball) using different DNA extraction methods (CTAB method, SDS potassium acetate method, SDS miniprep method and urea phenol method). The highest amount of DNA (349μg/g of leaves) was extracted using CTAB method, while it was lowest (130 μg/g of leaves) in SDS miniprep method. The absorbance ratio was high for DNA extracted by CTAB method (1.82) followed by SDS- potassium acetate (1.65) and SDS miniprep (1.60) methods. For standardization of PCR ampl ification conditions, optimization was carried out to determine the optimal concentrations of template DNA, MgCl₂, Taq DNA polymerase and annealing temperature. In general, either no products or products with inconsistent bands were obtained when the concentration of template DNA was 25 ng or MgCl₂ was 1.0 mM with high annealing temperature of 45°C.

Horse

The scapula of horse is a flat triangular bone placed at the shoulder region applied against the cranial part of the lateral wall of thorax similar to that in ox. The scapular spine is placed a little further caudad from the cranial border so that the proportion of the area of supraspinous to infraspinous fossa is one third to two third of the lateral surface of the scapula. The subscapular fossa is deeper and presents well marked vascular impressions. The nutrient foramen is placed at the distal third of the infraspinous fossa. The tuber scapulae and the glenoid cavity are placed further apart. The glenoid cavity is oval in outline and its rim has a distinct notch on its craniomedial aspect. The coronoid process is better developed and the acromion process is absent.

The humerus in horse is directed obliquely distally and caudally forming an angle of about 55 degrees with the horizontal plane almost equaling to that in ox. The musculospiral groove on the shaft is deeper and more spirally curved. The nutrient foramen is placed on the distal third of the medial surface. The deltoid tuberosity is better developed. The medial and lateral tuberosities of the proximal extremity are more prominent. The intertuberal (bisipital) groove is divided by an intermediate ridge. The summit of the lateral tuberosity does not arch inward. The head is smaller than that in ox. The coronoid and olecranon fossae of the distal extremity are shallower than those in ox.

The radius of the horse is larger and longer of the two bones of the forearm. Close to the medial border of the caudal surface in the lower third of the shaft is a rough elevation for attachment of the superior check ligament (an accessory ligament of the superficial digital flexor). The caudal surface of the shaft towards its lateral border presents a rough triangular area which tapers to a point in the distal third of this surface. This is for attachment of the ulna. The distal interosseous space is absent because the shaft of ulna is shorter and does not reach to the distal part of the shaft of radius. The grooves on the cranial surface of the distal part of the shaft are better marked. The radial tuberosity at the craniomedial aspect of the proximal extremity is larger and more prominent than in ox. The distal articular surfaces are not oblique. It presents two tubercles one on each side, for ligamentous attachment. The tubercle on the lateral aspect is grooved for passage of tendon of lateral digital extensor.

1. UNIT OF CERTIFICATION

The entire area planted under seed production programme by an individual constitutes an unit, provided;

1. All the seed production programme is to produce seed of one category and one variety.
2. Area does not exceed 10 hectares and scattered fields constituting one unit (10 ha) should not be separated by more than 50 metres distance (Table 1).
3. The crop of the entire area is more or less of the same growth stage. The seed crop is grown as sole crop and it is not heavily and uniformly lodged.

2.1 INTRODUCTION

GIS is a tool that can be used in varied disciplines ranging from civil engineering to business. Remote sensing data is often brought into GIS to have a complete data set in many natural resource application areas. Full capability of GIS is not used widely. Many a times people use GIS only for digitising maps and to do simple query on maps. But it can also be used in modeling, simulation and forecasting, only then one may regard GIS technology is utilised to its full potential. Some GIS softwares handle simple problems for location based services while other complex softwares posses advanced modules to do all types of spatial, network and 3D analysis. Integration of softwares used in natural resources study are being interfaced with GIS to create scenarios.

Remote sensing and GIS technology is useful in the following fields:

Shoulder joint

The shoulder joint (Fig. 5-2 A) is an enarthrosis formed between the glenoid cavity of scapula and head of humerus.

Ligaments: The shoulder joint is enclosed by the capsular ligament which is loose enough to accommodate the wide range of movements of the joint and is attached about 1.25 cm away from the periarticular area of the two segments of the joint. The capsular ligament is supported externally by the muscles of the shoulder region.

Movements: The shoulder joint performs all kinds of movements but the extension and flexion are best marked.

13.1. Germplasm

Also known as genetic resources or gene pool or genetic stock.

Sum total of hereditary material i.e. all the allele of various genes, present in a crop species and its wild relatives

Importance of training and pruning

1. To control the habit of vegetative growth and regular bearing.
2. Remove all limbs and dead branches.
3. To improve flowering, fruiting and fruit quality
4. Remove all broken shoots, diseased, or pest infection branches from all trees and shrubs.
5. It is usually best to prune temperate fruits and shrubs during early spring before full leaf.
6. Maintain balance between shoots and roots at planting and nutrients uptake
7. Provide the basic tree form and to aid the development of a strong tree framework by encouraging strong wide crotch angles
8. Stimulate shoot growth near a cut.
9. Permit proper light distribution and penetration to the tree canopy.
10. Increase insect and disease control by allowing for better spray penetration.
11. Facilitate harvesting.
12. Achieve orchard uniformity.
13. Produce the proper amount of well distributed fruiting wood.
14. Encourage a structurally sound tree by removing branches with weak narrow crotch angles or those with diseased, damaged, or unproductive wood. Invigorate and renew the bearing area of older trees.

14.1 Basic Plotting: plot

This functionality on Series and DataFrame is just a simple wrapper around the matplotlib libraries plot() method.

Example

import pandas as pd

import numpy as np

df = pd.DataFrame(np.random.randn(10,4),index=pd.date_range(‘1/1/2000’, periods=10), columns=list(‘ABCD’))

df.plot()

Its output is as follows:

If the index consists of dates, it calls gct().autofmt_xdate() to format the x-axis as shown in the above illustration.

Agriculture economics is the study of how resources are used, distributed, and utilised in order to create agricultural products. A consistent level of farm surplus is one of the sources of technological and commercial expansion, so agricultural economics has an impact on the economics of development.

In general, it may be said that average salaries are low when a sizable portion of a nation’s population depends on agriculture for a living. It is more accurate to argue that a country’s majority of people must rely on agriculture because it is impoverished rather than that a country is poor because the majority of its population works in agriculture.

Features of Agriculture

Agriculture is the principal industry of employment. Nearly 61 percent of the whole population is employed there. It makes up 25% of the country’s income.

Monsoon dependence: The monsoon is crucial to Indian agriculture. The production will increase if the monsoon is good, and the crops will fail if it is below normal. Floods can occasionally ruin our crops. Agriculture depends on the rainfall since irrigation systems are so poor.

Abstract

This chapter explores farm budgeting, a method of evaluating plans for the allocation of agricultural resources at the decision-maker’s level. It involves estimating the income, costs, and profit of different farm activities.

Introduction The ecological stability and food web of intertidal environments are significantly influenced by macroalgae. The majority of marine organisms, including macroalgae, coral reefs, sponges, and the organisms that they are associated with, are well known for producing secondary metabolites and bioactive compounds, which increase their value in a variety of industries, including food, neutraceuticals, pharmaceuticals, and others. Among them, seaweeds play a vital role in promoting health by offering a variety of phytochemicals with antioxidant, antibacterial, anticancer, and antiviral action (Ngo et al. 2012). Worldwide, there have been more than 20,000 macroalgal species identified. In South Asian nations including China, Japan, and Korea, it has been used as sea vegetables. India now has 841 macroalgal species identified,including 434, 191, and 216 species of red algae, brown algae, and green algae, respectively (Oza and Zaidi, 2001).

19.1 Introduction

Quality has been defined as the properties of any given food that have an influence on the acceptance or rejection of this food by the consumer. Egg quality is a general term which refers to several aspects of both internal and external quality. Internal egg quality involves functional, aesthetic and microbiological properties of the egg yolk and albumen. The proportions of components for fresh egg are 32% yolk, 58% albumen and 10% shell. The quality of an egg is at its best when the egg is laid. There is no known method by which this quality can be improved subsequently.

Arrangement of plants and animals into groups according to their close resemblance, naming and describing them is called classification. Classifying the plants into groups is called systematic botany. The main aim of classification is to name the plant to describe them and to bring out their resemblances in order to throw light on their relationship and common ancestor. It is to indicate primitive and advanced plants systematically.

Need for Classification

It is not possible for anyone to study all the organisms. But if they are grouped in some convenient way the study would become easier as the characters of a particular group or a family would apply to all the individuals of that group. Classification allows us to understand diversity better.

Classification of plants

Entire plant kingdom is divided into four main groups.

1. Thallophyta 2. Bryophyta 3. Pteridophyta 4. Spermatophyta or Phenerogams

Types of Chemicals Used in Disease Management
1. Fungicides: Used to control fungal pathogens, fungicides can either prevent infection (protectant fungicides) or cure infections after they
occur (systemic fungicides). Examples include copper-based fungicides like copper oxychloride and sulfur-based compounds.
2. Bactericides: These are designed to control bacterial pathogens. Common examples include streptomycin and copper compounds, which act against bacterial blights and spots. However, bactericides are less widely used than fungicides due to limited availability of effective products.
3. Nematicides: These chemicals target nematodes, which cause root diseases in crops. Carbofuran & oxamyl are commonly used nematicides.
4. Fumigants: These are volatile chemicals applied to soil to kill a wide range of soil-borne pathogens, including fungi, bacteria, and nematodes. Examples include methyl bromide and chloropicrin.
Contact vs. Systemic Chemicals
1. Contact Pesticides: These chemicals remain on the surface of plants and must come into direct contact with pathogens to be effective. They are most effective as preventative measures.
2. Systemic Pesticides: These are absorbed by the plant and move through its tissues, offering protection from within. Systemic fungicides, such as triazoles, are curative, meaning they can halt an infection after it has started.

Impact assessment approaches
Impact assessment approaches can be classified into four broad categories i.e. quantitative, qualitative, participatory and mixed methods.
i. Quantitative impact assessment approaches
• It focuses on assessing the degree and extent of the impacts quantitatively.
• Some degree of quantification may be necessary in all impact assessments, in order to evaluate the success of the Intervention and the extent of any adverse effects.
• Largely depends on micro-economic approaches following econometric models
• Involving baseline studies: The precise identification of baseline conditions, definition of objectives, target setting, rigorous performance evaluation and outcome measurement
• Costly and limited scope: Limited in the types of impacts which can be accurately measured and may pose difficulties for inference of cause and effect.

Genus : Fragaria
Species : ananassa Duch.
Family : Rosaceae 
Chromosome No. : 2n=14, 25, 42, 56

Strawberry is relatively small genus comprising of usually low perennial creeping herbs, wild forms found wide spread in temperate and subtropical parts of the world. Cultivated strawberry is highly polymorphic and wide range of diversity present in the wild germplasm has resulted in the development of present day strawberry. Today it is grown throughout the world and is rated high in the list of liked foods. The breeders have contributed enormously towards the development of cultivars with wide adaptability and through the incorporation of desired traits helped to improve its quality dramatically over its wild forms. Today strawberry is known for its distinct delicate flavour, attractive fruit shape and colour and has made a special niche in the soft fruits culture. The cultivated strawberry Fragaria x ananassa Duch has resulted from the crossing of two local America species F. chiloensis (L) Duch. and F.virgianana, Duch.

ABSTRACT

Though the genetic control of sex determination is being explored in several animal systems, no much information is available on the genetic basis for sex specificities in dioecious or sexually polymorphic plant species. Date palm is a unique member of the family Palmaceae which exhibits dioecy. Here we report the sex-specific variation in the genomic DNA of male and female date palms as revealed from an extensive study conducted with palms representing different date palm growing regions of the Kingdom of Saudi Arabia. The genomic DNA content in females was less than 60 per cent compared to those of the male genotypes. Polymerase chain reactions carried out to amplify different segments of the alcohol dehydrogenase (AdhA) gene from the date palm genomic DNA yielded two DNA fragments each in the female genotypes where as the same primers amplified a single fragment in male plants. Genomic Southern analysis of Eco R1 restriction digests showed RFLP between the male and female genotypes with the male plants showing a single hybridization signal of approximately 14 kb size and the females showing six additional bands of varying molecular weights. The results of our investigations reveal the potential of molecular biology in differentiating the sex in date palm at DNA level. Further research is needed to develop DNA markers that will help to unequivocally identify the sex of dioecious plants at the seedling stage.

3.1. Downy mildew of peas
It is an important disease of pea crop particularly in those areas where cool and wet climate is prevalent during its cultivation. The disease during favourable weather conditions causes considerable yield losses.
Pathogen: Peronospora viciae (formerly P. pisi)
Host range
Vigna faba, Vigna sativa. Pisum arvensis, P.sativum, Lathyrus sativus, Vicia hirsuta, Vicia sativa, Vicia villosa (Dixon, 1981).
Geographical distribution
Reports of the pathogen are from different countries on different crop host.
On Pea- Berkeley in England first discovered the disease in 1846 (Chupp and Sherf, 1960) and since then it has been found in all parts of the world where the crop is grown intensively. The occurrence of the disease has been reported from Argentina, Australia, Canada, Chile, Denmark, France, Germany, Kenya, Netherland, New Zealand, Rhodesia, Spain, Tasmania, USA, Ukrain, Italy, Poland and Australia (Dixon,1981). In India it is prevalent in Indogangetic plains including Uttar Pradesh and Punjab (Bains and Dhaliwal, 1986).