eChapters

Introduction

The immense use of chemicals and pharmacological entities has put the public health at brisk of potential health hazards. These substances in modern days are presented in the form of common constituents of food, medicines, beverages and junk foods etc. However, long term consumption of these substances might be responsible for chronic toxicity, but the accidental ingestion of large quantities of these substances may result into adverse acute toxic reactions. Hence an experimenter should have the acumen to develop and perform the acute toxic tests in order to find out the lethal dose 50 i.e. LD50 of the substances.

Acute Toxicity Studies

Acute toxicity studies helps to establish the dose dependent adverse effect including mortalities. The LD50 has been used as a major parameter in screening of pharmacological agents for acute toxic potential. Apart from mortality, other biological effects and the time of onset, duration and degree of recovery on survived animals, are also important in evaluation of acute toxicity. Hence, acute toxicity study solely gives information about LD50, therapeutic index and the degree of safety of a pharmacological agent.

Acute toxicity is defined as adverse effects occur following oral or dermal administration of a single dose of a substance, or multiple doses given within 24 hours, or an inhalation exposure of 4 hours. Oral administration is the most common form of acute systemic toxicity testing.

Abstract

Pulses being nutritionally vital, is an important constituent of diet for a very large number of peoples. A part from being a relatively cheaper source of dietary protein, it is also an important source of calories, certain essential amino acid, minerals and vitamins. Post harvest scenario of pulse shows that 75 per cent of production was milled in pulses with as dehusked split i.e. dal. Traditionally green gram and black gram dal is dehulled manually after soaking in water. Present investigation was carried out to develop the wet type dehuller and study was carried out on water absorption characteristics of green gram and black gram. The result reveals that the dehulling efficiency of abrasion type mechanism was about 95 per cent and 92 per cent in case of shearing type mechanism. It has been observed that rate of water absorption was initially high and then gradually decreases i.e. 0.54 to 0.18 and 0.48 to 0.18 for black and green gram, respectively. 

21.1 Introduction

The egg industry has grown quickly in the last decade as people’s eating habits have become more aware of the importance of good and nutritious diets. Eggs are now widely accepted as a good source of high-quality protein. Increased demand in consumer choice has resulted in a wide variety of egg selection available in the retail market. Eggs of high quality are needed to sustain niche markets for specialty and designer eggs–seeking consumers. Egg quality defines those characteristics of an egg that affect consumer acceptability and preference. The parameters for egg grading include shell cleanliness, strength, texture, and shape; the relative viscosity of the albumen; and the shape and firmness of the yolk.

History and thorough physical examination is crucial procedures in making diagnosis in horses. Mental attitude of a horse may help in understanding whether it has a localized problem or is systemically ill. Horse is better approached by its left side, keeping himself/herself next to the shoulder. Examination of the horse should be started in a non-threatening, easy, and quiet way. Generally physical examination starts at the head to prevent possible injury to unfamiliar examiner. Abnormalities in demeanor and mentation can be assessed by watching the horse’s initial response to the environment. The locomotor and neurologic systems are examined by observing the horse’s movements and gait at walk and trot, both on straight line and in turns, and backing. Horse weight should be taken. General inspection of the horse should be conducted by standing back keeping a safe distance and looking at the horse for it condition, abdominal shape, muscle symmetry,

Genus   : Rubus
Species : idaelus L. (European Red
                Raspberry) strigorus Michx. 
                (Red Raspberry occidentalis L. 
                (Black Raspberry)
Family : Rosaceae
Chromosome No. : 2n = 14 
                            (variable 28, 42, 56, 84)

The blackberries, raspberries, dewberries, boysenberry are collectively referred to as bramble fruits.  Mostly found in the north hemisphere. They belong to the family Rosaceae and genus Rubus. The family has nearly 250 species besides also has large number of apomictic segregates. Usually shrub, the woody species has biennial canes i.e. in the first year remains vegetative and in the second year it fruits and dies. Leaves variable in form from simple leaf to pinnately or palmately compound.  Flowers usually occur in clusters and are variable in colour, white pink to red.  Fruit is an aggregate of number of small drupes.

Green revolution technologies such as greater use of synthetic agrochemicals like fertilizers and pesticides, adoption of nutrient-responsive, high-yielding varieties of crops, greater exploitation of irrigation potentials etc. has boosted the production output in most cases. However, continuous use of these high energy inputs indiscriminately now leads to decline in production and productivity of various crops as well as deterioration of soil health and environments.

22.1 What is Livestock Product Marketing?

Livestock Products Marketing is the change of ownership of livestock derived food products. It links the livestock production and food consumption. There are three aspects of livestock market transactions:

1. Spatial – transactions occur across space

2. Temporal – transactions occur across time

3. Form – transactions occur in a certain form

Primary Dicot Stem

A stem with only primary permanent tissues is called primary stem. It represents a young stem. The different tissues present in dicot stem are arranged in concentric layers. e.g. Sunflower

I) Epidermis

Epidermis is the outermost layer of the stem. It is made up of compactly arranged parenchymatous cell, which look rectangular in transverse section. The cells are transparent and devoid of chloroplasts. The outer walls are convex, thickened and cutinized. On the outer side they possess a layer of cuticle. The internal walls of the epidermal cells are thin. The radial walls are thick towards the outer side and gradually become thin towards the inner side. Pits occur in the radial walls. The epidermis of sunflower bears several unbranched multicellular hair. At place the epidermis contains minute pores called stomata or stoma. Each stomata or stoma has a pair of specialized kidney shaped cells called guard cells. The guard cells have a few chloroplasts. By their swelling, the two guard cells can form a pore between them.

Introduction
The sun is the primary source of energy for all life on Earth. The primary energy source for the majority of ecosystems is limited to this one. Photosynthesis is performed by green plants, certain types of algae, and microorganisms. They utilize solar energy to fuel chemical reactions that combine basic inorganic components to create intricate organic chemicals. Elements undergo a cyclical process as they move through the different compartments of an ecosystem.
Photosynthesis is the process by which solar energy is captured and transformed into food in the form of chemical energy. Food comprises fundamental elements such as carbon, hydrogen, nitrogen, and other necessary elements, along with stored energy. The components present in the food undergo recycling. The flow of energy and nutrients begins with the producers, then moves to the consumers, and ultimately returns to the producers through the activity of decomposers. Energy, in contrast to materials, is not subject to recycling. Food traverses the food web, resulting in energy dissipation. Typically, just a mere 10% of the energy accumulated in a certain trophic
level, such as the producers, is effectively passed on to the subsequent trophic level, such as the herbivores. This is referred to as the energy pyramid. Ultimately, the energy available in the trophic level becomes so depleted that it cannot sustain any higher trophic levels. Consequently, the presence of fourth order consumers in any ecosystem is extremely rare.
The biotic component, often known as the life component of the environment, encompasses a diverse array of plants, animals, and bacteria, which form interconnected communities.

Introduction

India’s population is projected to be around 1,350 million by 2020 AD which requires food grain production of 343 million tonnes. Considering the current food grain production levels, India needs to raise food grain growth by not less than 4 percent. On the other hand the available per capita cultivable land has declined from 0.48 ha to 0.14 ha and by 2050 it may further decline to 0.07 ha i.e. for every 14 persons only one ha land will be available to produce all essential items like food, fiber, fuel, fodder, fruits etc. (Harshal et al., 2006). About 75 percent of 105 million farm families belong to small and marginal farmers with less than 2 ha and 40 percent of the rural population lives below poverty line. We often talk about self-sufficiency in food supply but the fact is that population living below poverty line does not have the purchasing power.

Atmosphere serves as the medium through which pollutants are transported and dispersed. While being transported, the pollutants undergo chemical reactions, which may be lethal or scavenged by chemical or physical process/method. But it all exclusively depends upon the meteorological conditions (Lee et .al, 1994). Historic air pollution episodes reveal that atmospheric conditions influence the pollutant concentrations as well as their toxicity (Goldsmith & Friberg, 1976; Wichmann et.al, 1989 and Pope et.al, 1992). However, the location, duration of release, height as well as the pollutant concentrations are also important, which effect the secondary pollutant transformation under the influence of meteorological conditions, but the atmospheric behavior is independent of their sources (Holzworth, 1974).

Abstract

The Mesozoic sediments contribute around 54% of the oil and 44% of the gas reserves of the world (Bois et al. 1982). Indian Mesozoic basins occupy an area of about 400 x 103 sq. km and are characterized as frontier basins under category II - IV. These basins are mostly overlain by the Deccan Traps of Late Cretaceous age and are least explored. Deccan Syneclise is one of the largest Mesozoic basins in India, covering an area of ~ 273 x 103 sq. km. Geophysical studies have inferred hidden sub-trappean Mesozoic sediments with thickness up to 2.5 km. It is considered that requisite heat generation due to Deccan Trap volcanism soon after the Cretaceous sedimentation may have acted as a catalyst in hydrocarbon generation. Surface geochemical prospecting surveys along with carbon isotopic studies have been used to assess hydrocarbon generation potential of this part of the basin. The area adjoining Nandurbar beneath Narmada-Tapti region of Deccan Syneclise was selected for geochemical studies where geophysical studies have shown considerable sediment thickness. Surface geochemical studies indicate the generation of light gaseous hydrocarbons, C1 and SC2+ in the range of 3 to 1187 ppb and 1 to 1449 ppb, respectively. The carbon isotopic signatures of selected soil gas samples dC13 CH4 in the range of - 24 to -39.4‰ PDB) suggest thermogenic origin

Abstract

The control of micronutrient deficiency is a realizable goal, notwithstanding the magnitude of the task, challenges and constraints that need to be resolved. Since wheat products particularly maida and atta form the major raw material in widely consumed food items like chapati, bread and biscuit. Fortification of flour as followed in many other countries could be an important strategy to help realize this goal. The technology of flour fortification is effective and cost affordable. Effective flour fortification program would require collaboration between scientific community, governmental and non-governmental agencies, and industry and consumer groups.

Genus : Prunus / Amygdalus
Species :  dulcis L. communis, Fritsch.
Family : Rosaceae
Chromosome No. : 2n = 28

Almond tree amongst the deciduous temperate fruits is the first crop to bloom in spring as it responds readily to warm temperature and also for its relatively low chilling requirements. The almond is thought to be one of the oldest nut crop used by man since very long.  However, its specific climatic requirements have greatly restricted its commercial production to limited regions of the world.  The almond blossoms are large attractive pink coloured very sensitive to spring frost, but at the same time the nut require high heat quantum at maturity, cool rainy weather during flowering restricts cross pollination and induces fungal infections. In the mild winter regions almond trees are limited by the fact that distinct chilling requirement for growth and flowering are not met with.

1. Introduction

Ornamentals include plants which have either attractive foliage or flowers. They are used for various purposes like bedding plants in the garden or as cut-flowers and have huge commercial importance in terms of nursery business. New cultivars of ornamentals, which have been developed by various plant improvement techniques (hybridization, mutation breeding and colchiploidy) are always an added attraction to the plant lovers and a highly prized commodity for commercialization.

New cultivars of ornamentals usually inherit characters of both the parents singly or in combination and produce an entirely new trait. The progenies, thus, express a range of phenotypic characters. One has to screen these plants and identify elite plant(s) having desired phenotypic characters.

Second part of this process is characterization of the selected plants. Various characters of these plants are studied as per laid down procedures, which include vegetative, floral, seed and rhizome characters. The recorded observations are then compared with the parental characters to establish their distinctiveness. Subsequently, these plants are subjected to field trials for studying their uniformity and stability of the desired phenotypic characters.

New cultivars of ornamentals have enough potential for commercialization and good source of earning on the part of breeders who may be farmers, nurseryman, and researchers. However, for claiming right over the new cultivars developed, it is now mandatory to get those new varieties registered with appropriate agencies. The registration authorities ask for every morphological details as well as parental history. Therefore, characterization is a must for the newly developed cultivars/varieties.

ABSTRACT

Piper colubrinum is a wild relative of the cultivated species of black pepper, Piper nigrum. The enzyme hydroxy methyl glutaryl Co A reductase which catalyses mevalonate synthesis, is the key enzyme in isoprenoid biosynthesis.This is the rate limiting step in the pathway which leads to the formation of sesquiterpene phytoalecins, a group of plant defense metabolites. Piper colubrinum is known to be tolerant to the dreaded disease called foot rot, incited by Phytophthora capsici. The hmgr gene is reported to confer resistance to fungal pathogens. Hence we made an attempt to isolate gene encoding this enzyme. cDNA was synthesised by reverse transcription from total RNA of P.colubrinum using oligo dt primers. Specific degenerate primers designed based on the conserved boxes among various plant species were used for amplifying hmgr gene from the cDNA. The 700 bp amplicon was cloned in pGEMT vector and sequenced with T7 primer. Multiple sequence alignment using clustal W 1.8 revealed homology of the cDNA fragment with hmgr genes in several other plant species. Maximum similarity was observed among P. colubrinum, Solanum xanthocarpum and Nicotiana tabacum. The cDNA library of P. colubrinum constructed in lTriplEx2 when screened with radiolabelled hmgr amplicon, yielded positive signals. Attempts are being made to isolate full length gene from these clones, which could be later used for imparting biotic stress tolerance to cultivated crop species.

6.1. Downy mildew of Sugarcane

Pathogen: Sugarcane downy mildew can be caused by four species:

    1)    Peronosclerospora sacchari
    2)    P. miscanthi
    3)    P. spontanea and
    4)    P. philippinensis

Major Host
Hosts in the Saccharum genus include: S. officinarum, S. robustum, S. spontaneum and S. edule.

Minor Host
Other hosts include Zea mays (maize), teosinte (Euchlaena mexicana), Sorghum halapense (Johnson grass), S. sudanense (Sudan grass), Tripsacum dactyloides (Gama grass), Sorghum bicolor (broom corn). Other studies suggest there are 18 species that are systemically infected in the sub-family Panicoideae; these include species of Andropogon, Bothriochloa, Eulalia, Schizachyrium and Sorghum (Bonde & Peterson 1981)

Geographic Distribution
The disease is restricted to the Pacific, South Asia and South East Asian regions viz Fiji, India, Indonesia, Japan, Papua New Guinea, Philippines, Taiwan and Thailand (Suma & Magarey 2000).

18.1 Library Introduction

This library contains modules for various types of functionalities. Some commonly use module of python standard library are:

(i) math module: which provides mathematical function to support difference types of calaculations.

(ii) cmath modules: which provides mathematical function for complex number.

(iii) random module: which provides function generation random number.

(iv) staticstics module: which provides mathematical satical function.

(v) urllib module: which provides URL handling function so that you can access websites from within your program.

1. NmPy library, provides some advance math functionalities along with tools to create and manipulate numeric arrays.

2. Scipy library, provides algorithmic and mathematical tools for scientific calculations.

Recurrent selection is a method of breeding applicable to cross-pollinated crops, designed to concentrate favourable genes scattered among a number of individuals by selecting in each generation among the progeny produced by mating inter se of the selected individuals (or their selfed progeny) of the previous generation. The concept of recurrent selection grew out of need to increase the number of superior genotypes that could be obtained from breeding stock of corn. This could have been possible by increasing the frequency of superior genes in the gene pool and by increasing the chances for genetic recombination to occur. The original idea behind recurrent selection was proposed first by Hayes and Gerber in 1919 and independently by East and Jones in 1920. Jenkins (1940) was the first to describe the method in detail and Hull (1945) proposed the name ‘recurrent selection’. Each cycle of recurrent selection requires:

23.1 Introduction

Eggs are nutrient dense superfoods, naturally occurring vitamin pills. They contain a complete source of protein with all essential aminoacids and naturally occurring vitamin D. The adverse publicity of high cholesterol content in the egg limit their intake. Nowadays consumers need the products free from drugs/ pesticides and contain special health promoting substances. To reap the growing demand of health conscious consumers and to make available of their requirements, designer eggs are made. Designer eggs are those eggs their composition differ from the standard eggs by changing the feed of the hen.